Method for constructing cardiac pressure overload/unloading mouse model

Abstract

The invention relates to a method for constructing a cardiac pressure overload / unloading mouse model. The invention proposes a new stable overload / unloading animal model construction method by carrying out animal experimental research. The conventional practice of constructing a pressure overload model is to directly knot the aortic arch when it is narrowed, which has poor stability. The improvement of the present invention is characterized in that two small PE10 catheters are used, and the two ends of a 7-0 surgical line firstly pass through a PE10 catheter simultaneously, and then one of theends separately passes through another PE10 catheter, and finally the two ends are subjected to knotting. The improved method solves the problem of difficulty finding the knotting position and difficultly unraveling when the aortic arch is reinstated, significantly reduces the phenomenon of large bleeding during the operation, improves the operability and reliability of the experiment, reduces the difficulty of model construction, and improves the stability and uniformity of the model. The method provides animal models that are more stable and more in line with clinical features for the studyof related diseases.

Description

technical field

[0001] The invention relates to a method for constructing a cardiac pressure overload / unload mouse model, which provides a reliable animal model for studying reverse cardiac remodeling. Background technique

[0002] Aortic arch stenosis accounts for a large proportion of adult heart diseases and is one of the main causes of heart failure. Chronic aortic stenosis is a common clinical disease that leads to left ventricular overload. Relief of outflow tract obstruction through aortic valve replacement is Typical unloading process. When the aortic valve is stenotic, the ejection resistance of the left ventricle increases, resulting in excessive left ventricular pressure load. The body's compensatory mechanism promotes myocardial hypertrophy to enhance the contractility of the ventricular muscle, increasing the pressure gradient across the aortic valve during systole, and maintaining normal cardiac output. This kind of compensation promotes the increase of wall ...

Examples

Embodiment Construction

[0019] Below by way of specific implementation, the above-mentioned content of the present invention is described in further detail again; But this should not be interpreted as the scope of the above-mentioned theme of the present invention being limited to following examples; All technologies realized based on the idea of ​​the present invention all belong to the present invention range. The concrete technical scheme that the present invention provides is as follows:

[0020] see figure 1 , Cardiac pressure overload / unload mouse model construction:

[0021] The specific operation steps are as follows

[0022] 1) Clean up the surgical area and prepare surgical instruments;

[0023] 2) The surgical area is wiped and disinfected with 75% alcohol;

[0024] 3) Make sure that the heat blanket is at the correct temperature (37°C), and make sure that the small animal ventilator is breathing smoothly. It is important to maintain normal body temperature and respiration in small an...

technical field

[0001] The invention relates to a method for constructing a cardiac pressure overload / unload mouse model, which provides a reliable animal model for studying reverse cardiac remodeling. Background technique

[0002] Aortic arch stenosis accounts for a large proportion of adult heart diseases and is one of the main causes of heart failure. Chronic aortic stenosis is a common clinical disease that leads to left ventricular overload. Relief of outflow tract obstruction through aortic valve replacement is Typical unloading process. When the aortic valve is stenotic, the ejection resistance of the left ventricle increases, resulting in excessive left ventricular pressure load. The body's compensatory mechanism promotes myocardial hypertrophy to enhance the contractility of the ventricular muscle, increasing the pressure gradient across the aortic valve during systole, and maintaining normal cardiac output. This kind of compensation promotes the increase of wall ...

Examples

Embodiment Construction

[0019] Below by way of specific implementation, the above-mentioned content of the present invention is described in further detail again; But this should not be interpreted as the scope of the above-mentioned theme of the present invention being limited to following examples; All technologies realized based on the idea of ​​the present invention all belong to the present invention range. The concrete technical scheme that the present invention provides is as follows:

[0020] see figure 1 , Cardiac pressure overload / unload mouse model construction:

[0021] The specific operation steps are as follows

[0022] 1) Clean up the surgical area and prepare surgical instruments;

[0023] 2) The surgical area is wiped and disinfected with 75% alcohol;

[0024] 3) Make sure that the heat blanket is at the correct temperature (37°C), and make sure that the small animal ventilator is breathing smoothly. It is important to maintain normal body temperature and respiration in small an...

Images