Biotechnology breeding method of regenerated functional rice

Abstract

The invention discloses a biotechnology breeding method of regenerated functional rice. The method comprises the following steps of S1, selecting barnyard grass as a donor; S2, selecting Xiangwanxian No.5 as a parent body; S3, extracting DNA of the donor; S4, introducing guiding the DNA of the donor into the Xiangwanxian No.5; S5, collecting distant hybridization seeds of the Xiangwanxian No.5 in S4. By means of the biotechnology breeding method, the yield of the rice can be greatly increased, the DNA of the barnyard grass is introduced into the Xiangwanxian No.5, and the purpose that rice is harvested twice after being planted once can be achieved. The barnyard grass belongs to field weeds, has strong growth capability, and does not grow tall but grows dense. The first yield of the rice seeds cultivated by means of the method reaches 550-600 kilograms, and the yield of the regenerated rice reaches 300-400 kilograms.

Description

technical field

[0001] The invention relates to a biotechnology breeding method for regenerative functional rice, belonging to the technical field of rice breeding methods. Background technique

[0002] About 60% of my country's population takes rice as the staple food, so increasing rice production is a major issue for my country's sustainable agricultural development and ensuring food security. As the process of industrialization and urbanization is engulfing rice fields, to meet the challenges of population growth and ensure the steady development of the national economy, the fundamental way out is to increase the yield potential per unit area. Grain is a special strategic commodity, which is related to national economy and people's livelihood, political stability and social stability. Once there is a shortage of food and the food problem cannot be solved, economic development and social stability will lose their solid foundation. At present and for a long period of tim...

Examples

Embodiment 1

[0031] Embodiment 1 of the present invention: a biotechnology breeding method for regenerative functional rice, comprising the following steps: S1: selecting barnyardgrass as the donor; S2: selecting Xiangwanxian No. 5 as the parent; S3: extracting the DNA of the donor ; S4: Introduce the DNA of the donor to select Xiangwanxian 5; S5: Collect the distant hybrid seeds of Xiangwanxian 5 in step S4; Step S6: Select and breed the distant hybrid seeds collected in step S5 for seven generations Obtain seeds of new lines with stable growth.

[0032] The method for extracting DNA comprises the following steps:

[0033] S31: Weigh 0.2 g of the first to fourth young leaves of the donor seedling, remove the DNA contamination on the surface, put them in a mortar and grind them together with liquid nitrogen to form a fine powder;

[0034] S32: Transfer the fine powder of the donor into a 2ml centrifuge tube, immediately add 1000 μl of CTAB extract preheated to 65°C, incubate at 65°C for 3...

technical field

[0001] The invention relates to a biotechnology breeding method for regenerative functional rice, belonging to the technical field of rice breeding methods. Background technique

[0002] About 60% of my country's population takes rice as the staple food, so increasing rice production is a major issue for my country's sustainable agricultural development and ensuring food security. As the process of industrialization and urbanization is engulfing rice fields, to meet the challenges of population growth and ensure the steady development of the national economy, the fundamental way out is to increase the yield potential per unit area. Grain is a special strategic commodity, which is related to national economy and people's livelihood, political stability and social stability. Once there is a shortage of food and the food problem cannot be solved, economic development and social stability will lose their solid foundation. At present and for a long period of tim...

Examples

Embodiment 1

[0031] Embodiment 1 of the present invention: a biotechnology breeding method for regenerative functional rice, comprising the following steps: S1: selecting barnyardgrass as the donor; S2: selecting Xiangwanxian No. 5 as the parent; S3: extracting the DNA of the donor ; S4: Introduce the DNA of the donor to select Xiangwanxian 5; S5: Collect the distant hybrid seeds of Xiangwanxian 5 in step S4; Step S6: Select and breed the distant hybrid seeds collected in step S5 for seven generations Obtain seeds of new lines with stable growth.

[0032] The method for extracting DNA comprises the following steps:

[0033] S31: Weigh 0.2 g of the first to fourth young leaves of the donor seedling, remove the DNA contamination on the surface, put them in a mortar and grind them together with liquid nitrogen to form a fine powder;

[0034] S32: Transfer the fine powder of the donor into a 2ml centrifuge tube, immediately add 1000 μl of CTAB extract preheated to 65°C, incubate at 65°C for 3...