Animal operant behavior experimental device and its use method

Abstract

The present invention discloses an animal operant behavior experiment device and a method for using the same. The animal operant behavior experiment device of the present invention comprises: a behavior operation panel, a buckle is provided on the behavior operation panel, a behavior operation device and a signal light are provided on the front of the behavior operation panel; an external stimulation device is provided to give external stimulation to the animal in the animal cage; and an I / O circuit board, the behavior operation device is electrically connected to the input end of the I / O circuit board, and the external stimulation device and the signal light are electrically connected to the output end of the I / O circuit board respectively. The animal operant behavior experiment device of the present invention is provided with a behavior operation device and a signal light on the front of the behavior operation panel, and can give external stimulation to the animal in the animal cage through the external stimulation device. When in use, it is only necessary to fix and lock the device on the original animal cage through the buckle, without transferring the animal from the animal cage to the test device, thereby realizing operant behavior training and testing in an independent animal cage.

Description

Technical Field

[0001] The present invention relates to the technical field of animal experimental equipment, and in particular to an animal operant behavior experimental device and a use method thereof. Background Art

[0002] Operant behavior training and testing are widely used in neurobiological research, and can be used to study learning, memory, reward, anxiety, drug addiction, motivation, etc. At present, there are relatively mature devices and technologies in rodents, and there are standard operant behavior Skinner boxes, but there are no mature devices in larger animals, such as pigs, dogs and non-human primates.

[0003] Existing operant behavior Skinner boxes are widely used in research on small animals such as rodents, and usually require animals to be placed in behavior boxes for training and testing. However, due to the large size of animals such as pigs, dogs and non-human primates, they cannot be tested using behavioral devices currently on the market. Summary of the invention

[0004] One object of the present invention is to provide an animal operant behavior experimental device, which can be used for operant behavior training and testing of large animals.

[0005] Another object of the present invention is to provide a method for using an animal operant behavior experimental device.

[0006] To achieve this object, on the one hand, the present invention adopts the following technical solutions:

[0007] An animal operant behavior experimental device, comprising:

[0008] A behavior operation panel, wherein the behavior operation panel is provided with a buckle capable of fixing the behavior operation panel on the animal breeding cage, and the front of the behavior operation panel is provided with a behavior operation device and a signal light;

[0009] an external stimulation device for giving external stimulation to the animals in the animal cage; and

[0010] An I / O circuit board is fixedly arranged on the back of the behavior operation panel, the behavior operation device is electrically connected to the input end of the I / O circuit board, and the external stimulation device and the signal light are electrically connected to the output end of the I / O circuit board respectively.

[0011] In some embodiments, the behavior operation panel is further provided with a clamping piece, and the clamping piece is provided with a clamping slot that can be tightly matched with the cage bars of the animal breeding cage; and / or,

[0012] The signal light comprises a first signal light and a second signal light, and the light colors of the first signal light and the second signal light are different.

[0013] In some embodiments, the behavioral operation device is connected to the input end of the I / O circuit board via a wire, the external stimulation device and the signal light are respectively connected to the output end of the I / O circuit board via wires, and all the wires are sheathed with metal protective tubes.

[0014] In some embodiments, the animal operant behavior experimental device further comprises a computer, wherein the computer is electrically connected to the I / O circuit board via a wire, and the outer portion of the wire is sheathed with a metal protective tube.

[0015] In some embodiments, the material of the metal protection tube is the same as the material of the animal cage.

[0016] In some embodiments, the behavioral manipulation device is a pressure lever or a nose probe; and / or,

[0017] The external stimulation device includes one or more of a drug injection device, a food delivery device, a liquid supply device, a plantar electrical stimulation device, a jet stimulation device, an optogenetic stimulation device, and an intracranial electrical stimulation device.

[0018] In some embodiments, the I / O circuit board is placed in a first protection box, and the first protection box is fixedly arranged on the back of the behavior operation panel; and / or,

[0019] The external stimulation device is placed in a second protection box, and the material of the second protection box is the same as that of the animal breeding cage.

[0020] On the other hand, the present invention adopts the following technical solutions:

[0021] A method for using an animal operant behavior experimental device, the method comprising the following steps:

[0022] Fix the animal operant behavior experimental device to the animal cage;

[0023] The first signal light is on, the operator applies a preset action to the behavioral operation device, and the external stimulation device outputs a food reward. After the reward, the first signal light is off; repeat multiple times;

[0024] The first signal light is on, inducing the animal to perform the preset action on the behavioral operation device, and repeating it multiple times;

[0025] The first signal light is on, and if the animal applies the preset action to the behavioral operation device, the first signal light is off, the second signal light is on, and the external stimulation device outputs a food reward, and the second signal light is off after the reward; repeat multiple times;

[0026] The first signal light is on, and when the animal continuously applies the preset action to the behavioral operation device for a preset number of times, the first signal light is off, the second signal light is on, and the external stimulation device outputs a food reward, and the second signal light is off after the reward; this is repeated multiple times;

[0027] Setting a unit time, and recording the number of times the external stimulation device outputs food rewards within the unit time, until the number of times the external stimulation device outputs food rewards within the unit time becomes stable;

[0028] After use, the animal operant behavior experimental device is removed from the animal cage.

[0029] In some embodiments, after the number of times the external stimulation device outputs food rewards per unit time is stable, the method further includes the following steps:

[0030] The food reward of the external stimulation device is replaced by drug injection, plantar electrical stimulation, air jet stimulation, optogenetic stimulation or intracranial electrical stimulation to conduct animal neural circuit, animal neural mechanism and animal behavior research.

[0031] In some embodiments, the behavioral operation device is a pressure bar, and the preset action is pressing the pressure bar.

[0032] The present invention has at least the following beneficial effects:

[0033] The animal operant behavior experimental device of the present invention fixes an I / O circuit board on the back of a behavior operation panel to prevent it from being damaged by animals, and arranges a behavior operation device and a signal light on the front of the behavior operation panel, and can give external stimulation to animals in animal cages through external stimulation devices. When in use, the device only needs to be fixed and locked on the original animal cage by a ring buckle, and there is no need to transfer the animal from the animal cage to the test device, which simplifies and facilitates the experimental operation, realizes operant behavior training and testing in an independent animal cage, and carries out research on learning, memory, reward, anxiety, drug addiction, motivation, etc.

[0034] The method for using the animal operant behavior experimental device of the present invention greatly accelerates the animals' understanding of operant training, the standardized training method improves the animals' cooperation under pressure training, and the positive reinforcement and gradual process greatly shortens the training time. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 A schematic diagram of the partial structure of an animal operant behavior experimental device provided in an embodiment of the present invention;

[0036] Figure 2 A schematic diagram of the structure of an animal operant behavior experimental device and an animal breeding cage provided in an embodiment of the present invention;

[0037] Figure 3 A flow chart of a method for using the animal operant behavior experimental device provided in an embodiment of the present invention;

[0038] Description of Figure Numbers:

[0039] Behavior operation panel 10 , engaging member 11 , behavior operation device 20 , first signal light 31 , second signal light 32 , external stimulation device 40 , first protection box 50 , wire 60 , computer 70 , animal breeding cage 100 . DETAILED DESCRIPTION

[0040] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. Preferred embodiments of the present invention are provided in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0041] like Figure 1 and Figure 2 As shown, an animal operant behavior experimental device of one embodiment includes a behavior operation panel 10, an external stimulation device 40 and an I / O circuit board (i.e., an input and output circuit board), wherein the behavior operation panel 10 is provided with a buckle that can fix the behavior operation panel 10 on the animal breeding cage 100, and the front of the behavior operation panel 10 is provided with a behavior operation device 20 and a signal light; the external stimulation device 40 is used to give external stimulation to the animal in the animal breeding cage 100; the I / O circuit board is fixedly arranged on the back of the behavior operation panel 10, the behavior operation device 20 is electrically connected to the input end of the I / O circuit board, and the external stimulation device 40 and the signal light are respectively electrically connected to the output end of the I / O circuit board.

[0042] The above-mentioned animal operant behavior experimental device, by fixing the I / O circuit board on the back of the behavior operation panel 10 to avoid being damaged by the animal, sets the behavioral operation device 20 and the signal light on the front of the behavior operation panel 10, and can give external stimulation to the animal in the animal cage 100 through the external stimulation device 40. When in use, it is only necessary to fix (connect) the device to the original animal cage 100 through a ring buckle, and there is no need to transfer the animal from the animal cage 100 to the test device, which simplifies and facilitates the experimental operation, and realizes operant behavior training and testing in an independent animal cage 100, so as to carry out research on learning, memory, reward, anxiety, drug addiction and motivation.

[0043] It is understandable that the buckle provided on the behavior operation panel 10 can very conveniently lock the behavior operation panel 10 on the animal breeding cage 100, and can effectively prevent it from being disassembled by some smarter animals. Since the animal manipulation behavior experiment device of the present invention can be directly installed on the animal breeding cage 100, the animal manipulation behavior experiment device can not only realize the behavior training during the experimental period, but also realize the behavior monitoring of animals 24 hours a day.

[0044] Furthermore, the behavior operation panel 10 is also provided with a snap-fitting piece 11, which is provided with a slot adapted to the cage bars of the animal breeding cage 100, and the cage bars of the animal breeding cage 100 can be snapped into the slot and tightly matched with the slot. Among them, "cage bars" refer to the strips constituting the animal breeding cage 100. By designing the snap-fitting piece 11 with the above structure, the connection strength between the behavior operation panel 10 and the animal breeding cage 100 can be further strengthened, and the difficulty of removing the behavior operation panel 10 from the animal breeding cage 100 can be increased.

[0045] Specifically, in Figure 1 In the illustrated embodiment, the behavior operation panel 10 is rectangular, and a locking piece 11 is respectively provided on two adjacent sides of the behavior operation panel 10. When it is necessary to lock the operation panel 10 on the animal breeding cage 100, the cage bars of the animal breeding cage 100 are firstly clamped in the clamping grooves of the two locking pieces 11, and then the buckle is locked on the animal breeding cage 100.

[0046] Optionally, in some embodiments, the I / O circuit board is placed in a first protection box 50, and the first protection box 50 is fixedly arranged on the back of the behavior operation panel 10. By placing the I / O circuit board in the first protection box 50, the animal is further prevented from scratching and damaging the I / O circuit board.

[0047] In some embodiments, the signal light includes a first signal light 31 and a second signal light 32, and the light colors of the first signal light 31 and the second signal light 32 are different. Signal lights of different light colors can give different signals to animals, and train animals to perform corresponding operation behaviors according to the color of the signal light. Optionally, in this embodiment, the first signal light 31 can be a green light, and the second signal light 32 can be a red light. Of course, in other embodiments of the present invention, the first signal light 31 and the second signal light 32 can also be lights of other light colors, not limited to the above-mentioned green light and red light.

[0048] In some embodiments, the behavioral operation device 20 is connected to the input end of the I / O circuit board through a wire 60, and the external stimulation device 40 and the signal light are respectively connected to the output end of the I / O circuit board through wires 60, and a metal protective tube is provided on the outside of all wires 60. The life of captive animals (such as monkeys) is dull and boring, and the animal manipulation behavior experiment device greatly improves the various sensory stimulations of the animals. Animals may produce a variety of behavioral patterns out of boredom, such as disassembly, biting, shaking, etc., which leads to the destruction of the device. Therefore, the present invention can prevent animals from biting the wires 60 by providing metal protective tubes on the outside of all wires 60, thereby ensuring the integrity of the device circuit.

[0049] In some embodiments, the animal operant behavior experimental device further comprises a computer 70, which is electrically connected to the I / O circuit board via a wire 60, and a metal protective tube is provided on the outside of the wire 60. The user can use the computer 70 to set device parameters and programs, and to record and analyze experiments through the computer 70.

[0050] In some embodiments, the material of the metal protection tube is the same as that of the animal cage 100. By using the metal protection tube of the same material as that of the animal cage 100, the device line (wire) can be hidden, reducing the attention of the animal and preventing the animal from biting the wire 60. Optionally, the animal cage 100 is usually made of steel, so the metal protection tube can be made of the same steel pipe as that of the animal cage 100.

[0051] In some embodiments, the behavioral manipulation device 20 is a pressure bar or a nose probe. It is understandable that the behavioral manipulation device 20 can be selected according to the species of the animal to be trained and tested. For example, for some animals that like to use their noses to test objects (such as pigs, dogs, mice, etc.), a nose probe can be set as the behavioral manipulation device 20, while for some animals that like to use their limbs to test objects (such as monkeys, etc.), a pressure bar can be set as the behavioral manipulation device 20.

[0052] Optionally, the external stimulation device 40 includes one or more of a drug injection device, a food delivery device, a liquid supply device, a plantar electrical stimulation device, a jet stimulation device, an optogenetic stimulation device, and an intracranial electrical stimulation device. Specifically, the behavior operation panel 10 can be arbitrarily combined with a variety of external stimulation devices 40 to study various animal operating behaviors. Of course, the external stimulation device 40 is not limited to the above-mentioned several types, and other external stimulation devices 40 can also be selected in other embodiments, which can be specifically selected according to the test items and research directions.

[0053] Specifically, the drug injection device is a drug injection pump; the food delivery device is a granular food feeder; the liquid supply device includes a water outlet pipe and a liquid storage device controlled by a solenoid valve, and the liquid can be drinking water or beverages such as juice; the plantar electrical stimulation includes a metal bar at the bottom of the animal cage 100 and an electrical stimulator connected to the metal bar; the jet stimulation device is a compressed gas release device controlled by a solenoid valve; the optogenetic stimulation device is an external laser and its optical fiber; the intracranial electrical stimulation device is an external electrical stimulator and its wires and electrodes.

[0054] Optionally, some openings may be provided on the behavior operation panel 10 so as to realize the combination of the behavior operation panel 10 with external stimulation devices 40 such as a liquid supply device, a jet stimulation device, and a food delivery device.

[0055] In some embodiments of the present invention, the external stimulation device 40 is placed in a second protection box, and the material of the second protection box is the same as that of the animal cage 100. By placing the external stimulation device 40 in the second protection box, the animal is prevented from destroying the external stimulation device 40. By using a second protection box made of the same material as the animal cage 100, the second protection box can be hidden, reducing the animal's attention and preventing the animal from biting or moving the second protection box. Furthermore, the second protection box can be placed at a height higher than the length of the animal's arm to prevent the animal from disassembling it. It should be noted that when designing the second protection box, hardware products such as screws should be avoided as much as possible to prevent animals from screwing and eating the screws.

[0056] like Figure 3 As shown, the method for using the above-mentioned animal operant behavior experimental device includes the following steps:

[0057] S110 , fixing the animal operant behavior experiment device to the animal breeding cage 100 . Specifically, the behavior operation panel 10 is fixed to the animal breeding cage 100 through the buckles on the behavior operation panel 10 , and the external stimulation device 40 is fixed to the top of the breeding cage 100 .

[0058] S120, the first signal light 31 is on, the operator applies a preset action to the behavior operation device 20, the external stimulation device 40 outputs a food reward, and the first signal light 31 is off after the reward; repeat multiple times. In some embodiments, the behavior operation device 20 is a lever, and the preset action is pressing the lever.

[0059] S130, the first signal light 31 is on, inducing the animal to perform a preset action on the behavioral operation device 20, and repeating this multiple times. The purpose of this step is to make the animal know how to perform a preset action on the behavioral operation device 20, and the animal can be induced by food rewards during the specific operation.

[0060] S140, the first signal light 31 is on, if the animal applies a preset action to the behavioral operation device 20, the first signal light 31 is off, the second signal light 32 is on, and the external stimulation device 40 outputs a food reward, and the second signal light 32 is off after the reward; repeat multiple times. Specifically, step S140 is repeated multiple times until the animal actively uses the behavioral operation device 20 to perform the preset action when the first signal light 31 is on.

[0061] S150, the first signal light 31 is on. When the animal continuously applies the preset action to the behavioral operation device 20 for a preset number of times, the first signal light 31 is off, the second signal light 32 is on, and the external stimulation device 40 outputs a food reward. After the reward, the second signal light 32 is off; repeat multiple times, wherein the preset number of times is ≥2.

[0062] Specifically, step S150 is repeated multiple times until the animal uses the behavioral operation device 20 to perform a preset action for a preset number of times when the first signal light 31 is on. Optionally, the preset number of times can be set according to specific needs, and the present invention is not limited to this.

[0063] S160, set a unit time, and record the number of times the external stimulation device 40 outputs food rewards in the unit time, until the number of times the external stimulation device 40 outputs food rewards in the unit time is stable. When the number of times the external stimulation device 40 outputs food rewards in the unit time is stable, it means that the animal has successfully established the preset operation behavior, so the food reward of the external stimulation device 40 can be replaced with other stimuli in the future, and the animal's animal neural circuit, animal neural mechanism and animal behavior research can be carried out on the animal.

[0064] S170, after use, the animal operant behavior experimental device is removed from the animal breeding cage 100.

[0065] It is understandable that in some other operant behavior experiments (such as drug addiction experiments), step S170 may be omitted, that is, the animal operant behavior experiment device is not removed from the animal cage 100 to achieve 24-hour behavioral monitoring of the animal.

[0066] In some embodiments, after the number of times the external stimulation device 40 outputs food rewards per unit time is stable, the following steps are also included: S180, replacing the food rewards of the external stimulation device 40 with drug injection, plantar electrical stimulation, air jet stimulation, optogenetic stimulation or intracranial electrical stimulation, and conducting animal neural circuits, animal neural mechanisms and animal behavior research. Since food rewards can better stimulate the learning desire of animals, the method of using food rewards in the initial test can better help animals learn a certain operation behavior, which is convenient for later changing to other stimulations to carry out animal neural circuits, animal neural mechanisms and animal behavior research.

[0067] The following uses the example of a macaque as an animal and the behavioral manipulation device 20 as a pressure lever to describe the method of using the above-mentioned animal manipulation behavior experimental device in detail:

[0068] A. Fix the animal operant behavior experimental device to the macaque's cage;

[0069] B. The first signal light 31 is on, the operator presses the lever, the external stimulation device 40 outputs food reward, and the first signal light 31 is off after the reward; repeat multiple times;

[0070] C. The first signal light 31 is on, and the operator presses the pressure bar from the front and back to induce the macaque to press the pressure bar, and repeats this process several times so that the macaque learns how to press the pressure bar;

[0071] D. The first signal light 31 is on. If the macaque presses the lever, the first signal light 31 goes out, the second signal light 32 turns on, and the external stimulation device 40 outputs a food reward. After the reward, the second signal light 32 goes out. Repeat this process several times until the macaque actively presses the lever when it sees the first signal light on.

[0072] E. The first signal light 31 is on. When the number of times the animal continuously presses the lever reaches a preset number (e.g., five times), the first signal light 31 is off, the second signal light 32 is on, and the external stimulation device 40 outputs a food reward. After the reward, the second signal light 32 is off. This is repeated many times until the macaque actively presses the lever to a preset number of times when it sees the first signal light on.

[0073] F. Set 10 minutes as a unit time, and record the number of times the external stimulation device 40 outputs food rewards within 10 minutes, record 3 times a day, and record for 3 consecutive days until the number of times the external stimulation device 40 outputs food rewards within 10 minutes is stable;

[0074] G. After use, remove the animal operant behavior experimental device from the macaque's cage.

[0075] In summary, the method of using the animal operant behavior experimental device of the present invention greatly accelerates the animals' understanding of operant training, the standardized training method improves the animals' cooperation under pressure training, and the positive reinforcement and gradual process greatly shortens the training time.

[0076] It should be noted that when a part is referred to as being "fixed to" another part, it may be directly on the other part or there may be a central part. When a part is considered to be "connected" to another part, it may be directly connected to the other part or there may be a central part at the same time. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by technicians in the technical field of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0077] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0078] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for using an animal manipulative behavior experimental device, characterized in that: The device comprises: A behavior operation panel (10), wherein the behavior operation panel (10) is provided with a buckle capable of fixing the behavior operation panel (10) on the animal breeding cage (100), and a behavior operation device (20) and a signal light are provided on the front of the behavior operation panel (10); an external stimulation device (40) for giving external stimulation to the animal in the animal cage (100); and An I / O circuit board, the I / O circuit board being arranged on the back of the behavior operation panel (10), the behavior operation device (20) being electrically connected to an input end of the I / O circuit board, and the external stimulation device (40) and the signal light being electrically connected to output ends of the I / O circuit board respectively; The behavior operation panel (10) is further provided with a clamping piece (11), and the clamping piece (11) is provided with a clamping groove capable of clamping with the cage bars of the animal breeding cage (100); one clamping piece (11) is respectively provided on two adjacent sides of the behavior operation panel (10), and when it is necessary to lock the behavior operation panel (10) on the animal breeding cage (100), the cage bars of the animal breeding cage (100) are first clamped in the clamping grooves of the two clamping pieces (11), and then the buckle is locked on the animal breeding cage (100); The signal light comprises a first signal light (31) and a second signal light (32), and the light colors of the first signal light (31) and the second signal light (32) are different; The method comprises the following steps: Fixing the animal operant behavior experimental device to the animal cage (100); The first signal light (31) lights up, the operator applies a preset action to the behavioral operation device (20), and the external stimulation device (40) outputs a food reward, and after the reward, the first signal light (31) turns off; this is repeated multiple times; The first signal light (31) is on, inducing the animal to perform the preset action on the behavioral operation device (20), and repeating the process multiple times; The first signal light (31) is on, and if the animal applies the preset action to the behavioral operation device (20), the first signal light (31) is off, the second signal light (32) is on, and the external stimulation device (40) outputs a food reward, and the second signal light (32) is off after the reward; repeat multiple times; The first signal light (31) is on, and when the animal continuously applies the preset action to the behavioral operation device (20) for a preset number of times, the first signal light (31) is off, the second signal light (32) is on, and the external stimulation device (40) outputs a food reward, and the second signal light (32) is off after the reward; this is repeated multiple times; Setting a unit time, and recording the number of times the external stimulation device (40) outputs food rewards within the unit time, until the number of times the external stimulation device (40) outputs food rewards within the unit time becomes stable; After use, the animal operant behavior experimental device is removed from the animal breeding cage (100).

2. The method for using the animal manipulative behavior experimental device according to claim 1, characterized in that: The behavioral operation device (20) is connected to the input end of the I / O circuit board via a wire (60), and the external stimulation device (40) and the signal light are respectively connected to the output end of the I / O circuit board via wires (60), and the outside of all the wires (60) is provided with a metal protective tube.

3. The method for using the animal manipulative behavior experimental device according to claim 1, characterized in that: The animal operant behavior experimental device also includes a computer (70), wherein the computer (70) is electrically connected to the I / O circuit board via a wire (60), and the wire (60) is sheathed with a metal protective tube.

4. The method for using the animal manipulative behavior experimental device according to claim 2 or 3, characterized in that: The material of the metal protection tube is the same as that of the animal breeding cage (100).

5. The method for using the animal manipulative behavior experimental device according to claim 1, characterized in that: The behavioral manipulation device (20) is a pressure bar or a nose probe; and / or, The external stimulation device (40) comprises one or more of a drug injection device, a food delivery device, a liquid supply device, a plantar electrical stimulation device, a jet stimulation device, an optogenetic stimulation device and an intracranial electrical stimulation device.

6. The method for using the animal manipulative behavior experimental device according to claim 1, characterized in that: The I / O circuit board is placed in a first protection box (50), and the first protection box (50) is fixedly arranged on the back of the behavior operation panel (10); and / or, The external stimulation device (40) is placed in a second protection box, and the material of the second protection box is the same as that of the animal breeding cage (100).

7. The method for using the animal manipulative behavior experimental device according to claim 1, characterized in that: After the number of times the external stimulation device (40) outputs food rewards per unit time becomes stable, the following steps are also included: The food reward of the external stimulation device (40) is replaced by drug injection, plantar electrical stimulation, air jet stimulation, optogenetic stimulation or intracranial electrical stimulation to conduct animal neural circuit, animal neural mechanism and animal behavior research.

8. The method for using the animal manipulative behavior experimental device according to claim 1, characterized in that: The behavioral operation device (20) is a pressure rod, and the preset action is pressing the pressure rod.

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