A multifunctional experimental device for simulating soil microbial communities

Abstract

The invention discloses a multifunctional experimental device for simulating soil microbial communities, including an experimental table and supporting legs. A water receiving pool, a faucet, an incubator with an upper opening, an electronic scale and a microbial separation device are sequentially arranged along the length direction on the experimental table. A heating layer is arranged around the side wall of the incubator. At least one sampling hole is sequentially arranged along the height direction on one side of the incubator. The microbial separation device includes columns arranged parallel to each other on both sides. A placement groove is provided between the columns on both sides of the experimental table. A container is provided in the placement groove. A placement plate is inserted between the upper ends of the columns on both sides. A motor is provided in the middle of the upper end of the placement plate. The output shaft of the motor is connected to a stirring shaft extending from the lower end to the bottom of the container. A stirring blade is provided on the stirring shaft. A sampling valve is provided at the lower part of one side of the container. The advantages of the present invention compared with the prior art are: multiple functions, convenient and fast research, and improved speed and process of the experiment.

Description

Technical Field

[0001] The invention relates to the technical field of soil microorganism experiments, in particular to a multifunctional experimental device for simulating soil microorganism communities. Background Art

[0002] Soil microorganisms are a general term for all tiny organisms in the soil that are invisible or unclear to the naked eye. Strictly speaking, they should include bacteria, archaea, fungi, viruses, protozoa and microscopic algae. Their individuals are tiny, generally calculated in micrometers or millimeter. Usually, there are hundreds of millions to tens of billions of them in 1 gram of soil. Their types and numbers vary with the soil-forming environment and the depth of the soil layer. They carry out oxidation, nitrification, ammoniation, nitrogen fixation, sulfidation and other processes in the soil, promoting the decomposition of soil organic matter and the transformation of nutrients.

[0003] To know more about soil microorganisms, we need to conduct experiments on them to understand the specific factors affecting the soil environment on microorganisms. This requires the use of experimental equipment. Currently, the structure of traditional experimental equipment is relatively simple, and only a single experimental treatment can be carried out on it, which makes research more troublesome and affects the speed and progress of the experiment. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the above technical defects and provide a multifunctional experimental device for simulating soil microbial communities, which has diverse functions, is relatively convenient and quick in research, and improves the speed of the experiment.

[0005] In order to solve the above technical problems, the technical solution provided by the present invention is: a multifunctional experimental device for simulating soil microbial communities, comprising a laboratory table and supporting legs located at the four corners of the bottom of the laboratory table, wherein a water receiving tank, an incubator with an opening at the upper end, an electronic scale and a microbial separation device are sequentially arranged on the laboratory table along the length direction, a faucet is rotatably provided between the water receiving tank and the incubator, a heating layer is provided around the side wall of the incubator, and at least one sampling hole is sequentially provided on one side surface of the incubator along the height direction, the microbial separation device comprises columns arranged parallel to each other on both sides, a placement groove is opened on the laboratory table between the columns on both sides, a container is provided in the placement groove, a placement plate is inserted between the upper ends of the columns on both sides, a motor is provided in the middle of the upper end of the placement plate, the output shaft of the motor is connected to a stirring shaft whose lower end extends to the bottom of the container, a stirring blade is provided on the stirring shaft, and a sampling valve is provided at the lower part of one side of the container.

[0006] Compared with the prior art, the advantages of the present invention are as follows: soil samples from the same place are placed in an incubator, the soil samples in the incubator can be watered by turning a faucet to change the humidity of the soil samples, the soil in the incubator can be heated by a heating layer to change its temperature, soil samples of different depths can be taken out through sampling holes at different heights, soil samples of the same mass are weighed by an electronic scale and placed in a container, and the same amount of liquid is added to the container, and a soil suspension is made when a stirring shaft and a stirring blade are driven by a motor to rotate, and a soil suspension sample is collected by a test tube through a sampling valve, and microorganisms and soil are separated, so that the number and type of microorganisms in different humidity, temperature and soil depth can be well studied, the function is diverse, the research is relatively comprehensive and convenient and fast, and the speed of the experiment process is improved.

[0007] Preferably, the water outlet of the faucet is connected to a shower head, and the soil samples in the incubator are watered through the shower head, so that the watering is more uniform, thereby improving the accuracy of the final experiment.

[0008] Preferably, heating wires are evenly laid inside the heating layer, and a heat-insulating layer is provided outside the heating layer to reduce the heat loss rate and save energy.

[0009] Preferably, the incubator is provided with three sampling holes, and are respectively located at the upper, lower and middle parts of the incubator; a water receiving tray is fixedly connected to the lower end of a side surface of the incubator where the sampling holes are provided and is arranged obliquely corresponding to the sampling holes; the higher end of the water receiving tray is located at the lower end of the sampling holes at the lowest position, and the lower end extends to the upper side of the water receiving pool; the laboratory can take samples from the upper, middle and lower parts of the soil for research respectively, thereby increasing the diversity of the research; at the same time, when watering, the water and a small amount of soil flowing out of the sampling holes can fall onto the water receiving tray and flow into the water receiving pool, thereby keeping the laboratory bench clean and hygienic.

[0010] Preferably, the electronic scale is located between the incubator and the microorganism separation device, and the sample taken out from the sampling hole of the incubator can be directly placed on the electronic scale for weighing, which is more convenient and has a reasonable layout.

[0011] Preferably, slots are provided at the upper ends of the columns on both sides, and the lower ends of both ends of the placement plate are fixedly connected with rods inserted into the slots, and there is a clearance fit between the rods and the slots. The connecting plate is inserted into the slots through the rods and is detachably connected to the columns on both sides. When the container needs to be cleaned, the connecting plate and the motor can be removed, the stirring shaft with the stirring blades can be taken out of the container, and then the container can be taken out of the placement slot, which is convenient for cleaning and replacement of the container.

[0012] Preferably, both ends of the bottom surface of the laboratory table are respectively provided with a side-opening cabinet and a drawer cabinet fixedly connected to two adjacent supporting legs on the corresponding sides. The lower part of the water collection pool is located in the side-opening cabinet. The drawer cabinet can store some experimental tools, which are convenient for laboratory use and save time. At the same time, the lower part of the water collection pool and the connecting pipe, as well as the connecting pipe of the faucet are all located in the side-opening cabinet, and the appearance is more beautiful. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 The present invention is a schematic structural diagram of a multifunctional experimental device for simulating soil microbial communities.

[0014] As shown in the figure: 1. Laboratory table, 2. Support legs, 3. Water collection tank, 4. Incubator, 5. Faucet, 6. Shower head, 7. Sampling hole, 8. Heating layer, 9. Insulation layer, 10. Water collection tray, 11. Electronic scale, 12. Column, 13. Placement plate, 14. Motor, 15. Stirring shaft, 16. Stirring blade, 17. Container, 18. Sampling valve, 19. Placement slot, 20. Rod, 21. Side-opening cabinet, 22. Drawer cabinet. DETAILED DESCRIPTION

[0015] The present invention is further described in detail below in conjunction with the accompanying drawings.

[0016] A multifunctional experimental device for simulating soil microbial communities, comprising an experimental table 1 and supporting legs 2 located at the four corners of the bottom surface of the experimental table 1, wherein the experimental table 1 is provided with a water receiving pool 3, an incubator 4 with an upper opening, an electronic scale 11 and a microbial separation device in sequence along the length direction, wherein a faucet 5 is rotatably provided between the water receiving pool 3 and the incubator 4 on the experimental table 1, wherein the rotatable faucet 5 is an existing rotatable faucet, and at the same time, a drainage pipe at the lower end of the water receiving pool 3 is connected to a drainage channel reserved in advance, a heating layer 8 is provided around the side wall of the incubator 4, and at least one sampling hole 7 is sequentially provided on one side surface of the incubator 4 in the height direction, and the microbial separation device comprises two sides parallel to each other. The experimental table 1 has columns 12 arranged in rows, and a placement groove 19 is opened between the columns 12 on both sides. A container 17 is arranged in the placement groove 19, and the container 17 is a transparent glass container with scale for experiment. A placement plate 13 is inserted between the upper ends of the columns 12 on both sides, and a motor 14 is arranged in the middle of the upper end of the placement plate 13. The motor 14 is fixed to the middle of the placement plate 13 by screws, and the power input end of the motor 14 is connected to the external socket through a plug for power supply. The output shaft of the motor 14 is connected to a stirring shaft 15 whose lower end extends to the bottom of the container 17 through a coupling, and the stirring shaft 15 is provided with a stirring blade 16. A sampling valve 18 is provided at the lower part of one side of the container 17.

[0017] The water outlet of the faucet 5 is connected to a shower head 6 , and a water inlet pipe is provided at the lower end of the faucet 5 and is connected to a water supply pipe reserved in advance, and the water inlet pipe is located inside the side-opening cabinet 21 .

[0018] The interior of the heating layer 8 is evenly laid with heating wires, and the exterior of the heating layer 8 is provided with a heat-insulating layer 9. The heating layer 8 is actually a cavity formed between the side walls of the heating layer 8 and the side walls of the incubator 4. The heating wires are fixed to the inner wall of the cavity by a small pipe clamp, and the power supply end of the heating wire is connected to the external seat through a plug for power supply. At the same time, a temperature controller is installed on the connection circuit of the heating wire, so that the heating layer 8 can maintain a constant temperature. In addition, a layer of heat-insulating layer 9 is glued to the outer wall of the heating layer 8, and the sampling hole 7 passes through the heating layer 8, the heat-insulating layer 9 and the outer wall of the incubator 4. The aperture of the sampling hole 7 should not be too large to avoid a large amount of soil overflowing from the sampling hole. The incubator 4 is composed of stainless steel thin walls and is a vertically placed rectangular structure.

[0019] The incubator 4 is provided with three sampling holes 7, which are respectively located at the upper, lower and middle parts of the incubator 4. The incubator 4 is fixed with a water receiving tray 10 at the lower end of a side surface provided with the sampling holes 7 and arranged obliquely corresponding to the sampling holes 7. The higher end of the water receiving tray 10 passes through the insulation layer 9 and is welded to the outer wall of the heating layer 8 below the sampling holes 7 at the lowest position, and the lower end extends to the upper side of the water receiving pool 3.

[0020] The electronic scale 11 is located between the incubator 4 and the microorganism separation device.

[0021] Slots are provided at the upper ends of the upright posts 12 on both sides, and plug rods 20 inserted into the slots are fixedly connected to the lower ends of both ends of the placement plate 13, and there is a clearance fit between the plug rods 20 and the slots.

[0022] The two ends of the bottom surface of the experimental table 1 are respectively provided with a side-opening cabinet 21 and a drawer cabinet 22 fixedly connected to the two adjacent supporting legs 2 on the corresponding sides. The lower part of the water receiving pool 3 is located in the side-opening cabinet 21, and the side-opening cabinet 21 is the cabinet with side-opening doors that we often see.

[0023] When the present invention is implemented, soil samples from the same place are placed in an incubator. The soil samples in the incubator can be evenly watered through a shower nozzle by turning a faucet and opening a water valve to change the humidity of the soil samples. The water receiving tray can receive the water and the missing soil flowing out of the three sampling holes, and flow into the water receiving pool and be discharged through the drainage pipe at the lower end to keep the experimental table clean. The heating wire in the heating layer can also be energized and the required heating temperature can be adjusted through a set thermostat to heat the soil in the incubator to change its temperature. The soil samples can also be taken out through the three sampling holes at different heights. Samples of different depths in the upper, middle and lower parts are taken, and the same mass of soil samples are weighed by electronic scales, placed in containers, and the same amount of liquid is added to the containers, and soil suspension is made when the motor drives the stirring shaft and the stirring blades to rotate, and the soil suspension samples are collected by test tubes through sampling valves, and then placed on culture dishes for dilution, so that the colonies developed in the culture dishes can be well dispersed, and these separated microbial cells grow into dispersed colonies on the nutrient agar plate, and the number of microorganisms in the unit weight of soil is converted according to the number of colonies, and the types of microorganisms can be observed under a microscope. It can well study the number and types of microorganisms in different humidity, temperature and soil depths, with diverse functions, more comprehensive research, convenient and fast, and improve the speed of the experiment.

[0024] The present invention and its embodiments are described above, and such description is not restrictive. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it, without departing from the purpose of the invention, they can design a structure and embodiment similar to the technical solution without creativity, which should belong to the protection scope of the present invention.

Claims

1. A multifunctional experimental device for simulating soil microbial communities, comprising an experimental platform (1) and supporting legs (2) located at four corners of the bottom surface of the experimental platform (1), characterized in that: The experimental table (1) is provided with a water receiving pool (3), an incubator (4) with an opening at the top, an electronic scale (11) and a microorganism separation device in sequence along the length direction. A faucet (5) is rotatably provided on the experimental table (1) between the water receiving pool (3) and the incubator (4). A heating layer (8) is provided around the side wall of the incubator (4). Heating wires are evenly laid inside the heating layer (8). An insulating layer (9) is provided outside the heating layer (8). At least one sampling hole (7) is provided in sequence along the height direction on one side surface of the incubator (4). The microorganism separation device includes columns (12) arranged parallel to each other on both sides. A placement groove (19) is provided between the columns (12) on both sides of the experimental table (1). A container (17) is provided in the placement groove (19). A placement plate (13) is inserted between the upper ends of the columns (12) on both sides. The ends of the placing plate (13) are provided with slots, the lower ends of both ends of the placing plate (13) are fixedly connected with plug rods (20) inserted into the slots, and there is a clearance fit between the plug rods (20) and the slots. A motor (14) is provided in the middle of the upper end of the placing plate (13), and the output shaft of the motor (14) is connected with a stirring shaft (15) whose lower end extends to the bottom of the container (17), and the stirring shaft (15) is provided with a stirring blade (16). A sampling valve (18) is provided at the lower part of one side of the container (17). The incubator (4) is provided with three sampling holes (7), which are respectively located at the upper and lower parts and the middle part of the incubator (4). The incubator (4) is fixedly connected with a water receiving tray (10) arranged obliquely corresponding to the sampling holes (7) at the lower end of a side surface provided with the sampling holes (7), and the higher end of the water receiving tray (10) is located at the lower end of the sampling hole (7) at the lowest position, and the lower end extends to the upper side of the water receiving pool (3).

2. The multifunctional experimental device for simulating soil microbial communities according to claim 1, characterized in that: The water outlet end of the faucet (5) is connected to a shower head (6).

3. The multifunctional experimental device for simulating soil microbial communities according to claim 1, characterized in that: The electronic scale (11) is located between the incubator (4) and the microorganism separation device.

4. The multifunctional experimental device for simulating soil microbial communities according to claim 1, characterized in that: The two ends of the bottom surface of the experimental table (1) are respectively provided with a side-opening cabinet (21) and a drawer cabinet (22) fixedly connected to two adjacent supporting legs (2) on the corresponding sides, and the lower part of the water receiving pool (3) is located in the side-opening cabinet (21).

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