Photochemiluminescence detection device

Abstract

The present invention relates to a photochemiluminescence detection device, and relates to the technical field of chemiluminescence immunoassays. The device is used to address the technical problem in the prior art of false positive detection results due to the hook effect. The photochemiluminescence detection device of the present invention comprises an incubation device and a detection mechanism. The incubation device rotates to move the same test substance multiple times to a detection position, allowing the detection mechanism to perform multiple tests on the test substance, thereby determining whether the immunoassay has a hook risk. This avoids the possibility that the test sample cannot be correctly distinguished due to the hook effect, whether its concentration exceeds the linear range of the detection kit or is within the linear range, thereby avoiding experimental misdiagnosis.

Description

Technical Field

[0001] The present invention relates to the technical field of chemiluminescence immunoassay, in particular to a photoinduced chemiluminescence detection device. Background Art

[0002] Chemiluminescent immunoassay is a non-radioactive immunoassay technology that has developed rapidly in recent years. Its principle is to use chemiluminescent substances to amplify signals and directly measure the immune binding process based on their luminescence intensity. This method has become a key focus in immunological testing. In the double antibody sandwich detection model, when the concentration of the substance to be detected reaches a certain level, the double antibody sandwich complex cannot form, resulting in a low signal value. This phenomenon is called the high-dose hook effect (HD-HOOK effect). Existing chemiluminescent instruments are difficult to avoid the phenomenon of false positive test results due to this hook effect. Summary of the Invention

[0003] The present invention provides a photochemiluminescence detection device, which is used to solve the technical problem in the prior art that false positive detection results due to the HOOK effect cannot be avoided.

[0004] The present invention provides a photoinduced chemiluminescence detection device, comprising:

[0005] An incubation device, which is used to incubate the substance to be tested; the incubation device moves the same substance to be tested multiple times to the detection position through periodic motion;

[0006] The detection mechanism is arranged on one side of the incubation device and is used to perform multiple light excitations on the substance to be detected at the detection position and to detect the chemiluminescence generated by the substance to be detected after each light excitation.

[0007] In one embodiment, the detection mechanism includes an excitation part for emitting excitation light and exciting the object to be detected and a detection part for receiving and detecting a luminescence signal generated by the object to be detected.

[0008] In one embodiment, the excitation unit and the detection unit do not operate simultaneously.

[0009] In one embodiment, the excitation unit includes an exciter capable of emitting red excitation light of 600-700 nm.

[0010] In one embodiment, the detection unit includes a detector, and the detector is a single photon counter, a photomultiplier tube, a silicon photocell, or a photometric integrating sphere.

[0011] In one embodiment, the wavelength of the luminescent signal detectable by the detection unit is 520-620 nm.

[0012] In one embodiment, the detection mechanism is disposed above the incubation device.

[0013] In one embodiment, the excitation portion includes an excitation light path, and the detection portion includes a signal light path. The excitation light path and the signal light path are neither turned on nor turned off at the same time.

[0014] In one embodiment, a first switch for controlling the on or off of the excitation light path is provided on the excitation light path, and a second switch for controlling the on or off of the signal light path is provided on the signal light path, and the first switch and the second switch are inversely linked.

[0015] In one embodiment, the first switch and the second switch are respectively connected to two ends of a driving unit, and the driving unit causes the first switch and the second switch to be linked in opposite directions.

[0016] In one embodiment, the first switch includes a rotating shaft, and a first through hole is provided on the rotating shaft and passes through the rotating shaft in a radial direction. The first through hole is periodically aligned with the excitation light path.

[0017] In one embodiment, the second switch includes a crank mechanism, the crank mechanism is provided with a second through hole, and the second through hole is periodically aligned with the signal light path.

[0018] In one embodiment, the crank mechanism includes a first rotating plate and a second rotating plate hinged to each other, the first rotating plate is connected to the driving portion, and the second through hole is provided at a lower portion of the second rotating plate.

[0019] In one embodiment, the excitation portion further includes a second lens and a semi-transparent and semi-reflective lens, the exciter is arranged above the excitation light path, the second lens is arranged between the exciter and the first switch, and the semi-transparent and semi-reflective lens is arranged below the first switch.

[0020] In one embodiment, the detection unit further includes a first lens and a filter, the detector is arranged on one side of the signal light path, and the first lens and the filter are sequentially arranged between the semi-transparent and semi-reflective lens and the detector.

[0021] In one embodiment, the excitation light path and the signal light path are both provided on the housing, and the axis of the excitation light path is perpendicular to the axis of the signal light path.

[0022] In one embodiment, the shell includes a lower base arranged on the incubation device, an upper base fixed on the lower base, and a baffle arranged on the side of the lower base, the excitation light path passes through the upper base and the lower base, and the signal light path passes through the side wall of the lower base and the baffle.

[0023] In one embodiment, an exciter seat for fixing the exciter and a lens seat for fixing the second lens are provided on the upper base.

[0024] In one embodiment, the incubation device includes a reagent chamber for accommodating a reaction cup and a rotating component for driving the reagent chamber to rotate, and the detection mechanism is fixed at a detection position on the reagent chamber.

[0025] In one embodiment, a fixing device is provided inside the reagent chamber, the reaction cup is provided on the fixing device, and the fixing device rotates with the reagent chamber.

[0026] In one embodiment, the fixing device is disc-shaped, and fixing grooves are arranged at equal intervals on the periphery of the fixing device, and the reaction cup is arranged in the fixing grooves.

[0027] In one embodiment, the rotating component includes a support body, and a rotating shaft and a motor respectively provided on the support body, the motor is connected to the rotating shaft via a synchronous belt, and the rotating shaft is connected to the rotating connection portion at the bottom of the reagent chamber.

[0028] In one embodiment, a mounting hole is provided on the support body, and a support column connected to the bottom of the reagent chamber is provided in the mounting hole.

[0029] In one embodiment, the rotating component is provided with a positioning device for reading the position of the reagent chamber.

[0030] In one embodiment, the positioning device includes a sensor fixed on the support body and a sensor light shielding plate arranged at the bottom of the rotating shaft, wherein the sensor light shielding plate is flush with the height of the sensor and rotates with the rotating shaft.

[0031] Compared with the prior art, the advantages of the present invention are that the incubation device moves the same test substance to the detection position multiple times by rotating, so that the detection mechanism can perform multiple tests on the test substance, and then determine whether there is a HOOK risk in the immunoassay, thereby avoiding the HOOK effect caused by the inability to correctly distinguish the test sample due to its concentration exceeding the linear range of the detection kit or the concentration itself being this value, thereby avoiding experimental misdiagnosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Hereinafter, the present invention will be described in more detail based on embodiments with reference to the accompanying drawings.

[0033] Figure 1 3D diagram of the photochemiluminescence detection device according to an embodiment of the present invention;

[0034] Figure 2 2 is a top view of a photochemiluminescence detection device according to an embodiment of the present invention;

[0035] Figure 3 yes Figure 1 The three-dimensional structural diagram of the detection mechanism shown;

[0036] Figure 4 yes Figure 1 The front view of the detection mechanism shown;

[0037] Figure 5 yes Figure 4 A cross-sectional view at AA (hatching not shown) when the excitation light path in the detection mechanism is open;

[0038] Figure 6 yes Figure 4 A cross-sectional view at AA (hatching not shown) of the detection mechanism shown when the excitation light path is closed;

[0039] Figure 7 yes Figure 4 A cross-sectional view at BB (hatching not shown) when the signal light path in the detection mechanism is turned on;

[0040] Figure 8 yes Figure 4 The cross-sectional view at BB (the section line is not shown in the figure) is shown when the signal light path in the detection mechanism is closed.

[0041] In the drawings, like components are designated by like reference numerals, but the drawings are not necessarily drawn to scale.

[0042] Reference numerals:

[0043] 1-Incubation device; 2-Detection mechanism; 3-Excitation unit;

[0044] 4-Detection unit; 5-Housing; 6-Reagent compartment;

[0045] 7-rotating component; 8-positioning device; 31-excitation light path;

[0046] 32-first switch; 33-activator; 34-second lens;

[0047] 35-transmissive and semi-reflective lens; 41-signal light path; 42-second switch;

[0048] 43-detector; 44-first lens; 45-filter;

[0049] 51- driving part; 52- lower base; 53- upper base;

[0050] 54- baffle; 61- fixing device; 62- reaction cup;

[0051] 63-fixing groove; 71-support body; 72-rotating axis;

[0052] 73-motor; 74-synchronous belt; 75-support column;

[0053] 81-sensor; 82-sensor light shield; 321-rotating shaft;

[0054] 322 - first through hole; 421 - crank mechanism; 422 - second through hole;

[0055] 423 - first rotating plate; 424 - second rotating plate; 531 - exciter seat;

[0056] 532-lens seat; 711-mounting hole. DETAILED DESCRIPTION

[0057] The present invention will be further described below with reference to the accompanying drawings.

[0058] like Figure 1 and 2 As shown, the present invention provides a photoinduced chemiluminescence detection device, which includes an incubation device 1 and a detection mechanism 2, wherein the incubation device 1 is used to incubate the substance to be detected and move the same substance to be detected to the detection position multiple times through periodic movement; the detection mechanism 2 is arranged on one side of the incubation device 1, and is used to perform multiple photoexcitations on the substance to be detected at the detection position, and detect the chemiluminescence generated by the substance to be detected after each photoexcitation.

[0059] Specifically, the incubation device 1 moves the same substance to be tested to the detection position multiple times by rotating, so that the detection mechanism 2 performs multiple tests on the substance to be tested, and then determines whether there is a HOOK risk in the immunoassay.

[0060] Preferably, the detection mechanism 2 is arranged above the incubation device 1, so as to facilitate the emission of excitation light to the substance to be detected.

[0061] Optionally, the detection mechanism 2 is arranged on the side or bottom of the incubation device 1.

[0062] In some embodiments, the aforementioned detection position refers to the position where the detection mechanism is located (ie, the position where the excitation light is generated).

[0063] Of course, the above-mentioned detection position may also be the position on the incubation device 1 where the substance to be detected is located.

[0064] It should also be noted that the periodic motion described in the present invention includes rotation, linear reciprocating motion or swinging.

[0065] The detection mechanism 2 of the present invention will be described in detail below.

[0066] like Figure 3-8 As shown, the detection mechanism 2 includes an excitation part 3 for emitting excitation light and exciting the object to be detected and a detection part 4 for receiving and detecting the luminescence signal generated by the object to be detected. The excitation part 3 and the detection part 4 do not work at the same time.

[0067] In one embodiment, the excitation unit 3 includes an exciter 33 , and the exciter 33 can emit red excitation light of 600-700 nm.

[0068] Among them, the exciter 33 is arranged above the substance to be tested, and in the excitation part 3, except for the exciter 33 which does not move periodically with the incubation device 1, the other components of the excitation part 3 can move periodically with the incubation device 1, and the present invention is not limited to this.

[0069] In one embodiment, the detection unit 4 includes a detector 43 , wherein the detector 43 is a single photon counter, a photomultiplier tube, a silicon photocell, or a photometric integrating sphere.

[0070] The wavelength of the luminescence signal that can be detected by the detection unit 4 is 520 to 620 nm.

[0071] Similarly, in the detection part 4, except for the detector 43 which does not perform periodic movement with the incubation device 1, the other components of the detection part 4 can perform periodic movement with the incubation device 1, and the present invention is not limited to this.

[0072] Furthermore, if Figure 3 As shown, the excitation part 3 includes an excitation light path 31 , and the detection part 4 includes a signal light path 41 . The excitation light path 31 and the signal light path 41 are neither turned on nor turned off at the same time.

[0073] The excitation light path 31 is provided with a first switch 32 for controlling the on / off switching of the excitation light path 31, and the signal light path 41 is provided with a second switch 42 for controlling the on / off switching of the signal light path 41. The first switch 32 and the second switch 42 are inversely linked. This simultaneously drives the excitation light path 31 and the signal light path 41 on and off. Specifically, when the excitation light path 31 is on, the signal light path 41 is off; when the excitation light path 31 is off, the signal light path 41 is on.

[0074] Specifically, when the excitation light is required to excite the object to be tested, the driving part 51 rotates, and the driving part 51 drives the first switch 32 to rotate, and the excitation light path 31 is turned on (eg Figure 5 As shown), the driving unit 51 drives the second switch 42 to rotate, and the signal light path 41 is in the closed state (as shown). Figure 8 shown).

[0075] Similarly, when receiving and detecting the luminous signal generated by the object to be tested, the driving part 51 rotates again, and the driving part 51 drives the first switch 32 to rotate, and the first switch 32 blocks the excitation light path 31 (such as Figure 6 As shown), the driving unit 51 drives the second switch 42 to rotate, and the signal light path 41 is in the open state (as shown). Figure 7 As shown in FIG. 4 , the driving unit 51 controls the opening and closing of the excitation light path 31 and the signal light path 41 simultaneously.

[0076] like Figure 3 As shown, the first switch 32 and the second switch 42 are respectively connected to the two ends of the driving part 51, and the driving part 51 causes the first switch 32 and the second switch 42 to be linked in opposite directions.

[0077] The driving unit 51 is a rotating electromagnet or a motor. Output shafts are respectively provided at both ends of the driving unit 51 , one end of which is connected to the first switch 32 , and the other end of which is connected to the second switch 42 .

[0078] The first switch 32 includes a rotating shaft 321 . A first through hole 322 is provided on the rotating shaft 321 and passes through the rotating shaft 321 in a radial direction. The first through hole 322 is periodically aligned with the excitation light path 31 .

[0079] Specifically, if Figure 5 As shown, the driving part 51 drives the rotating shaft 321 to rotate. When the rotating shaft 321 rotates until the first through hole 322 thereon is aligned with the excitation light path 31, the excitation light path 31 is opened, and the excitation light emitted by the excitation part 3 can be irradiated on the substance to be tested; Figure 6 As described above, when the rotating shaft 321 rotates until the first through hole 322 thereon is misaligned with the excitation light path 31 , the excitation light path 31 is closed, and the excitation light emitted by the excitation unit 3 cannot irradiate the substance to be measured.

[0080] The second switch 42 includes a crank mechanism 421 . The crank mechanism 421 is provided with second through holes 422 . The second through holes 422 are periodically aligned with the signal light path 41 .

[0081] The crank mechanism 421 includes a first rotating plate 423 and a second rotating plate 424 which are hinged to each other. The first rotating plate 423 is connected to the driving portion 51 . The second through hole 422 is provided at the lower portion of the second rotating plate 424 .

[0082] Specifically, if Figure 8 As shown, when the driving portion 51 drives the second rotating plate 424 to rotate counterclockwise around its rotation center, the second rotating plate 424 drives the first rotating plate 423 to rotate clockwise around its rotation center, so that the second through hole 422 is aligned with the signal light path 41, and the signal light path 41 is conducted, and the light signal generated by the object to be tested enters the detection portion 4 for detection; Figure 7 As shown, the driving part 51 drives the second rotating plate 424 to rotate clockwise, so that the first rotating plate 423 rotates counterclockwise around its rotation center, and the second through hole 422 is misaligned with the signal light path 41, and the signal light path 41 is closed.

[0083] When the driving unit 51 drives the rotating shaft 321 to rotate clockwise, the first through hole 322 is aligned with the excitation light path 31, and at the same time the driving unit 51 causes the first rotating plate 423 to rotate counterclockwise, and the second through hole 422 is misaligned with the signal light path 41; similarly, when the driving unit 51 drives the rotating shaft 321 to rotate counterclockwise, the first through hole 322 is misaligned with the excitation light path 31, and at the same time the driving unit 51 causes the first rotating plate 423 to rotate clockwise, and the second through hole 422 is aligned with the signal light path 41, thereby ensuring that the process of detecting the luminous signal generated by the object to be detected and the process of the excitation light exciting the object to be detected do not interfere with each other, thereby improving the accuracy of the detection information.

[0084] like Figure 5 As shown, the excitation part 3 also includes a second lens 34 and a semi-transparent and semi-reflective lens 35. The exciter 33 is arranged above the excitation light path 31, the second lens 34 is arranged between the exciter 33 and the first switch 32, and the semi-transparent and semi-reflective lens 35 is arranged below the first switch 32.

[0085] Among them, the excitation light emitted by the exciter 33 excites the object to be tested multiple times, causing the object to generate multiple luminescence signals; the second lens 34 is used to focus the excitation light; the semi-transparent and semi-reflective lens 35 can not only pass the excitation light of the target wavelength and cut off the excitation light of non-target wavelengths, but also reflect the luminescence signal of the target wavelength generated by the object to be tested.

[0086] The detection unit 4 further includes a first lens 44 and a filter 45 . The detector 43 is disposed on one side of the signal light path 41 . The first lens 44 and the filter 45 are sequentially disposed between the semi-transparent and semi-reflective lens 35 and the detector 43 .

[0087] The detector 43 detects the luminescence signal generated by the object to be detected multiple times and records the corresponding detection results.

[0088] The luminous signal generated by the object under test, reflected by the transflective lens 35, passes through the first lens 44 and enters the detection unit 4. After being reflected by the transflective lens 35, the luminous signal generated by the object under test passes through the first lens 44 and the filter 45, and then enters the detection unit 4. The filter 45 can extract the signal of the desired wavelength from the luminous signal generated by the object under test and block stray light signals outside of this wavelength.

[0089] The excitation light path 31 and the signal light path 41 are both provided on the housing 5, and the axis of the excitation light path 31 is perpendicular to the axis of the signal light path 41. Figure 3 As shown, the axis L1 of the excitation light path 31 is along the Z-axis direction, and the axis L2 of the signal light path 41 is along the X-axis direction.

[0090] The shell 5 includes a lower base 52 arranged on the incubation device 1, an upper base 53 fixed on the lower base 52, and a baffle 54 arranged on the side of the lower base 52. The excitation light path 31 passes through the upper base 53 and the lower base 52, and the signal light path 41 passes through the side wall of the lower base 52 and the baffle 54.

[0091] Among them, the excitation light path 31 includes a first excitation light path (for example, a through hole) passing through the upper base 53 and a second excitation light path (for example, a through hole) passing through the lower base 52, and the axes of the first excitation light path and the second excitation light path coincide with each other; the signal light path 41 includes a first signal light path (for example, a through hole) passing through the side wall of the lower base 52 and a second signal light path (for example, a through hole) passing through the baffle 54, and the axes of the first signal light path and the second signal light path coincide with each other.

[0092] like Figure 4 As shown, the baffle 54 is disposed on one side of the lower base 52 , the second switch 42 is disposed between the baffle and the lower base 52 , and the detector 43 and the second switch 42 are disposed on the outer side and the inner side of the baffle 54 , respectively.

[0093] The end of the rotating shaft 321, which is provided with a first through-hole 322, passes through the upper base 53. The first through-hole 322 is disposed within the upper base 53, and the end of the rotating shaft 321 is rotatably connected to a sidewall of the upper base 53. When the excitation light needs to be controlled to excite the object under test, the driving unit 51 rotates, and the output shaft at its first end drives the rotating shaft 321 to rotate, so that the first through-hole 322 is aligned with the excitation light path 31. The excitation light emitted by the exciter 33 passes through the excitation light path 31 to excite the object under test (at this time, the signal light path 41 is closed).

[0094] In addition, an exciter seat 531 for fixing the exciter 33 and a lens seat 532 for fixing the second lens 34 are provided on the upper base 53 .

[0095] Preferably, when the analyte is a solution after a chemiluminescent immunoreaction, the excitation light emitted by the exciter 33 is used to excite the analyte twice to generate two chemiluminescent signals, and the detector 43 records the two chemiluminescent readings. After completing the two readings, the two readings are processed separately. When the increase in the second reading and the first reading is greater than the maximum value of the standard curve, it can be determined whether the immunoassay has a Hook risk. Based on the two chemiluminescent readings, and the difference increase between the second and first readings is recorded as A, a standard curve is made based on the first reading and the increase A of the two readings of a series of known standard substances containing the target antigen (or antibody) to be tested; the first reading and the increase A of the two readings of the analyte containing the target antigen (or antibody) to be tested are compared with the standard curve, so that the concentration of the analyte can be determined.

[0096] In addition, since the first switch 32 controlling the excitation light path 31 and the second switch 42 controlling the signal light path 41 are linked in opposite directions, it is possible to ensure that the excitation light excitation of the reactants in the reaction cup and the reactant luminescence signal detection process do not interfere with each other, thereby improving the accuracy of the reactant luminescence signal detection information and shortening the detection cycle. At the same time, the present invention can prevent the problems of time difference and jump hole detection from occurring during the detection process.

[0097] The incubation device 1 of the present invention will be described in detail below.

[0098] like Figure 1 and 2 As shown, the incubation device 1 includes a reagent chamber 6 for accommodating a reaction cup 62 and a rotating component 7 for driving the reagent chamber 6 to rotate. The detection mechanism 2 is fixed at a detection position on the reagent chamber 6.

[0099] A fixing device 61 is provided inside the reagent chamber 6 , and a reaction cup 62 is provided on the fixing device 61 . The fixing device 61 rotates along with the reagent chamber 6 .

[0100] The fixing device 61 is configured in a disk shape. Fixing grooves 63 are provided at equal intervals on the periphery of the fixing device 61 , and the cuvettes 62 are disposed in the fixing grooves 63 .

[0101] Furthermore, in order to keep the reaction cup 62 stable during the rotation following the reagent chamber 6 , a limiting block may be provided in each fixing groove 63 .

[0102] Optionally, the limiting block is an elastic protrusion provided on the inner side wall of the fixing groove 63 . When the reaction cup 62 is inserted into the corresponding fixing groove 63 , the elastic protrusion is compressed, thereby fixing the position of the reaction cup 62 .

[0103] like Figure 1As shown, an opening is provided on the side wall of the reagent chamber 6 , through which the reaction cup 62 can be placed into the fixing groove 63 .

[0104] The rotating component 7 includes a support body 71 and a rotating shaft 72 and a motor 73 respectively provided on the support body 71 . The motor 73 is connected to the rotating shaft 72 via a synchronous belt 74 . The rotating shaft 72 is connected to the rotating connection portion at the bottom of the reagent chamber 6 .

[0105] Specifically, the support body 71 is flat, and a timing belt 74 and a large and small timing pulley connected to the timing belt 74 are provided on the upper portion of the support body 71. The large timing pulley is connected to the rotating shaft 72, and the small timing pulley is driven by the motor 73. Of course, the rotating shaft 72 and the motor 73 can also be connected via a gear mechanism or a chain mechanism, which will not be described in detail here.

[0106] The support body 71 is provided with four mounting holes 711, which are respectively provided at the four corners of the support body 71. A support column 75 connected to the bottom of the reagent chamber 6 is provided in the mounting hole 711.

[0107] The rotating component 7 is provided with a positioning device 8 for reading the position of the reagent chamber 6 .

[0108] The positioning device 8 includes a sensor 81 fixed on the support body 71 and a sensor light shield 82 arranged at the bottom of the rotating shaft 72. The sensor light shield 82 is flush with the height of the sensor 81 and rotates with the rotating shaft 72.

[0109] The sensor 81 and the sensor light barrier 82 can determine the initial position of the turntable, and the required test container can be positioned to the current position via this initial position.

[0110] Specifically, the sensor 81 includes a zero position sensor and a position sensor arranged below the zero position sensor, and the sensor light shield 82 includes a zero position shield and a code disk, wherein the zero position shield and the code disk are respectively fixed at the upper end and the lower end of the large synchronization wheel. The zero position shield intermittently passes through the zero position sensor when the rotating shaft 72 rotates, and can be used to calibrate the zero position of the rotation; a plurality of grooves are arranged at equal angles on the code disk, each groove corresponds to a test container, and when the code disk rotates with the rotating shaft, different grooves pass through the position sensor respectively, and the position information of the corresponding test container can be obtained.

[0111] While the present invention has been described with reference to preferred embodiments, various modifications may be made and equivalent components may be substituted without departing from the scope of the present invention. In particular, the various technical features described in the various embodiments may be combined in any manner, provided no structural conflicts exist. The present invention is not limited to the specific embodiments disclosed herein, but encompasses all technical solutions within the scope of the claims.

Claims

1. A photochemiluminescence detection device, characterized in that: include: an incubation device, which is used to incubate the substance to be tested; The incubation device moves the same substance to be tested multiple times to the detection position through periodic motion; the periodic motion includes rotation, linear reciprocating motion or swinging; A detection mechanism is arranged on one side of the incubation device, and is used to perform multiple optical excitations on the substance to be tested in the detection position, and to detect the chemiluminescence generated by the substance to be tested after each optical excitation; the detection mechanism includes an excitation part and a detection part, the excitation part includes an excitation light path, and the detection part includes a signal light path, the excitation light path and the signal light path are not turned on or off at the same time; a first switch for controlling the conduction or closing of the excitation light path is provided on the excitation light path, and a second switch for controlling the conduction or closing of the signal light path is provided on the signal light path, and the first switch and the second switch are linked in reverse; when the excitation light path is turned on, the signal light path is closed; when the excitation light path is closed, the signal light path is turned on.

2. The photochemiluminescence detection device according to claim 1, characterized in that The excitation unit is used to emit excitation light and excite the object to be detected, and the detection unit is used to receive and detect the luminescence signal generated by the object to be detected.

3. The photochemiluminescence detection device according to claim 2, characterized in that: The excitation unit and the detection unit do not operate simultaneously.

4. The photochemiluminescence detection device according to claim 2 or 3, characterized in that: The excitation unit includes an exciter, and the exciter can emit red excitation light of 600-700 nm.

5. The photochemiluminescence detection device according to claim 4, characterized in that: The detection unit includes a detector, which is a single photon counter, a photomultiplier tube, a silicon photocell or a photometric integrating sphere.

6. The photochemiluminescence detection device according to claim 2 or 3, characterized in that: The wavelength of the luminescent signal that can be detected by the detection unit is 520 to 620 nm.

7. The photochemiluminescence detection device according to any one of claims 1 to 3, characterized in that: The detection mechanism is arranged above the incubation device.

8. The photochemiluminescence detection device according to claim 1, characterized in that: The first switch and the second switch are respectively connected to two ends of the driving unit, and the driving unit causes the first switch and the second switch to be linked in opposite directions.

9. The photochemiluminescence detection device according to claim 8, characterized in that: The first switch includes a rotating shaft, and a first through hole is provided on the rotating shaft and passes through the rotating shaft in a radial direction. The first through hole is periodically aligned with the excitation light path.

10. The photochemiluminescence detection device according to claim 8, characterized in that: The second switch includes a crank mechanism, the crank mechanism is provided with a second through hole, and the second through hole is periodically aligned with the signal light path.

11. The photochemiluminescence detection device according to claim 10, characterized in that: The crank mechanism includes a first rotating plate and a second rotating plate hinged to each other, the first rotating plate is connected to the driving part, and the second through hole is provided at the lower part of the second rotating plate.

12. The photochemiluminescence detection device according to claim 5, characterized in that: The excitation portion further includes a second lens and a semi-transparent and semi-reflective lens. The exciter is arranged above the excitation light path, the second lens is arranged between the exciter and the first switch, and the semi-transparent and semi-reflective lens is arranged below the first switch.

13. The photochemiluminescence detection device according to claim 12, characterized in that: The detection unit further includes a first lens and a filter. The detector is arranged on one side of the signal light path. The first lens and the filter are sequentially arranged between the semi-transparent and semi-reflective lens and the detector.

14. The photochemiluminescence detection device according to claim 12, characterized in that: The excitation light path and the signal light path are both arranged on the housing, and the axis of the excitation light path is perpendicular to the axis of the signal light path.

15. The photochemiluminescence detection device according to claim 14, characterized in that: The shell includes a lower base arranged on the incubation device, an upper base fixed on the lower base, and a baffle arranged on the side of the lower base. The excitation light path passes through the upper base and the lower base, and the signal light path passes through the side wall of the lower base and the baffle.

16. The photochemiluminescence detection device according to claim 15, characterized in that: The upper base is provided with an exciter seat for fixing the exciter and a lens seat for fixing the second lens.

17. The photochemiluminescence detection device according to any one of claims 1 to 3, characterized in that: The incubation device includes a reagent chamber for accommodating a reaction cup and a rotating component for driving the reagent chamber to rotate, and the detection mechanism is fixed at a detection position on the reagent chamber.

18. The photochemiluminescence detection device according to claim 17, characterized in that: A fixing device is provided inside the reagent chamber, the reaction cup is provided on the fixing device, and the fixing device rotates with the reagent chamber.

19. The photochemiluminescence detection device according to claim 18, characterized in that: The fixing device is disc-shaped, and fixing grooves are arranged at equal intervals on the periphery of the fixing device. The reaction cups are arranged in the fixing grooves.

20. The photochemiluminescence detection device according to claim 18, characterized in that: The rotating component includes a support body, and a rotating shaft and a motor respectively arranged on the support body. The motor is connected to the rotating shaft through a synchronous belt, and the rotating shaft is connected to the rotating connection part at the bottom of the reagent chamber.

21. The photochemiluminescence detection device according to claim 20, characterized in that: The support body is provided with a mounting hole, and a support column connected to the bottom of the reagent compartment is provided in the mounting hole.

22. The photochemiluminescence detection device according to claim 21, characterized in that: The rotating component is provided with a positioning device for reading the position of the reagent chamber.

23. The photochemiluminescence detection device according to claim 22, characterized in that: The positioning device includes a sensor fixed on the support body and a sensor light shielding plate arranged at the bottom of the rotating shaft. The sensor light shielding plate is flush with the height of the sensor and rotates with the rotating shaft.

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