Culture medium and culture method for tissue culture of blue tall cluster plantain lily

Abstract

The invention belongs to the technical field of plant tissue culture and particularly relates to a culture medium and a culture method for tissue culture of blue tall cluster plantain lily. The tissueculture method comprises the following steps of acquisition of sterile materials, induction of buds, proliferation culture, rooting culture and seedling hardening, wherein during proliferation culture, high-concentration hormones and low-concentration hormones are adopted for alternate proliferation, and high-proliferation proportion propagation is adopted, so that the proliferation ratio can beincreased, vitrification caused by accumulation of the high-concentration hormones in plant bodies is eliminated, and the proliferation rate of the blue tall cluster plantain lily is increased; and the tissue culture method is easy to operate, so that the production cost can be greatly lowered, and the problems of serious vitrification of seedlings, large callus tissues, difficulty in cutting dueto hard callus tissues and the like caused by adopting single high-concentration hormone in an existing tissue culture method are solved.

Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium and a culture method for tissue culture of hosta japonica.

[0002] technical background

[0003] Big-leaf blue hosta is a perennial herb with a plant height of 120cm. The leaves are gathered at the base, long and narrow, with fine teeth on the sides, and the panicles are large, feathery, silvery white. It is a famous ornamental grass abroad, used for landscaping or planting on the shore . Positive, likes a sunny, warm and humid environment, and is also cold-tolerant; strong adaptability, regardless of the soil, resistant to water and humidity, but also resistant to drought, and now there is a great demand in the market. At present, there are many domestic reports on the tissue culture research of large-leaf hosta , but there is an unstable multiplication ratio, difficult operation of long leaves during multiplication and transfer, diffic...

Examples

Embodiment 1

[0020] A kind of culture method of tissue culture of big leaf blue hosta, comprises the following steps:

[0021] (1) Obtaining sterile materials: Select the newly grown shoots as explants, remove the leaves wrapped outside the explants, soak in detergent water for 1 minute to wash away surface impurities, rinse with running water, and leave in an ultra- Cut off the wound with a scalpel on a clean workbench, disinfect with the prepared 0.08% mercuric chloride solution plus a drop of Tween for 10 minutes, rinse with sterile water for 4 times, and inoculate a small section of the sterilized explant into the Cultured on the prepared medium for 9 days;

[0022] (2) Bud induction: 15 days after the explants were inoculated on the axillary bud induction medium MS+NAA0.3mg / L+6-BA 2.5mg / L, the axillary buds began to expand and green protrusions appeared, and after 25 days, the buds were divided Tissues were cultured for another 25 days, and the small adventitious buds grew to 2-3 cm....

Embodiment 2

[0031] A kind of culture method of tissue culture of big leaf blue hosta, comprises the following steps:

[0032] (1) Obtaining sterile materials: Select the newly grown shoots as explants, remove the leaves wrapped outside the explants, soak in detergent water for 1 minute to wash away surface impurities, rinse with running water, and leave in an ultra- Cut off the wound with a scalpel on a clean workbench, disinfect with the prepared 0.09% mercuric chloride solution plus a drop of Tween for 9 minutes, rinse with sterile water for 5 times, and inoculate a small section of the sterilized explant into the Cultured on the prepared medium for 8 days;

[0033] (2) Bud induction: 13 days after the explants were inoculated on the axillary bud induction medium MS+NAA0.3mg / L+6-BA 2.75 mg / L, the axillary buds began to expand and green protrusions appeared, and after 23 days, the buds were divided Tissues were cultured for another 23 days, and the small adventitious buds grew to 2-3 cm...

Embodiment 3

[0042] A kind of culture method of tissue culture of big leaf blue hosta, comprises the following steps:

[0043] (1) Obtaining sterile materials: Select the newly grown shoots as explants, remove the leaves wrapped outside the explants, soak in detergent water for 1 minute to wash away surface impurities, rinse with running water, and leave in an ultra- Cut off the wound with a scalpel on a clean workbench, disinfect with the prepared 0.1% mercuric chloride solution plus a drop of Tween for 8 minutes, rinse with sterile water for 5 times, and inoculate small sections of the sterilized explants into the Cultured on the prepared medium for 8 days;

[0044] (2) Bud induction: 11 days after the explants were inoculated on the axillary bud induction medium MS+NAA0.3mg / L+6-BA 3.0mg / L, the axillary buds began to expand and green protrusions appeared, and after 20 days, the buds were divided Tissues, cultured for another 20 days, the small adventitious buds grow to 2~3cm, cut off th...

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium and a culture method for tissue culture of hosta japonica.

[0002] technical background

[0003] Big-leaf blue hosta is a perennial herb with a plant height of 120cm. The leaves are gathered at the base, long and narrow, with fine teeth on the sides, and the panicles are large, feathery, silvery white. It is a famous ornamental grass abroad, used for landscaping or planting on the shore . Positive, likes a sunny, warm and humid environment, and is also cold-tolerant; strong adaptability, regardless of the soil, resistant to water and humidity, but also resistant to drought, and now there is a great demand in the market. At present, there are many domestic reports on the tissue culture research of large-leaf hosta , but there is an unstable multiplication ratio, difficult operation of long leaves during multiplication and transfer, diffic...

Examples

Embodiment 1

[0020] A kind of culture method of tissue culture of big leaf blue hosta, comprises the following steps:

[0021] (1) Obtaining sterile materials: Select the newly grown shoots as explants, remove the leaves wrapped outside the explants, soak in detergent water for 1 minute to wash away surface impurities, rinse with running water, and leave in an ultra- Cut off the wound with a scalpel on a clean workbench, disinfect with the prepared 0.08% mercuric chloride solution plus a drop of Tween for 10 minutes, rinse with sterile water for 4 times, and inoculate a small section of the sterilized explant into the Cultured on the prepared medium for 9 days;

[0022] (2) Bud induction: 15 days after the explants were inoculated on the axillary bud induction medium MS+NAA0.3mg / L+6-BA 2.5mg / L, the axillary buds began to expand and green protrusions appeared, and after 25 days, the buds were divided Tissues were cultured for another 25 days, and the small adventitious buds grew to 2-3 cm....

Embodiment 2

[0031] A kind of culture method of tissue culture of big leaf blue hosta, comprises the following steps:

[0032] (1) Obtaining sterile materials: Select the newly grown shoots as explants, remove the leaves wrapped outside the explants, soak in detergent water for 1 minute to wash away surface impurities, rinse with running water, and leave in an ultra- Cut off the wound with a scalpel on a clean workbench, disinfect with the prepared 0.09% mercuric chloride solution plus a drop of Tween for 9 minutes, rinse with sterile water for 5 times, and inoculate a small section of the sterilized explant into the Cultured on the prepared medium for 8 days;

[0033] (2) Bud induction: 13 days after the explants were inoculated on the axillary bud induction medium MS+NAA0.3mg / L+6-BA 2.75 mg / L, the axillary buds began to expand and green protrusions appeared, and after 23 days, the buds were divided Tissues were cultured for another 23 days, and the small adventitious buds grew to 2-3 cm...

Embodiment 3

[0042] A kind of culture method of tissue culture of big leaf blue hosta, comprises the following steps:

[0043] (1) Obtaining sterile materials: Select the newly grown shoots as explants, remove the leaves wrapped outside the explants, soak in detergent water for 1 minute to wash away surface impurities, rinse with running water, and leave in an ultra- Cut off the wound with a scalpel on a clean workbench, disinfect with the prepared 0.1% mercuric chloride solution plus a drop of Tween for 8 minutes, rinse with sterile water for 5 times, and inoculate small sections of the sterilized explants into the Cultured on the prepared medium for 8 days;

[0044] (2) Bud induction: 11 days after the explants were inoculated on the axillary bud induction medium MS+NAA0.3mg / L+6-BA 3.0mg / L, the axillary buds began to expand and green protrusions appeared, and after 20 days, the buds were divided Tissues, cultured for another 20 days, the small adventitious buds grow to 2~3cm, cut off th...