Microbiological observation test card, observation system and observation method

Abstract

The present invention discloses a microorganism observation test card, comprising a transparent main card body and a transparent film, wherein the end face of the main card body is provided with a fluid introduction port, and the upper surface of the main card body is provided with a plurality of fluid channels, a plurality of observation slots and a plurality of sample pools, wherein the fluid channels, the observation slots and the sample pools are connected in sequence, and the depth of the observation slots is smaller than the depth of the sample pools; the film can cover the upper surface of the main card body and seal the fluid channels, the observation slots and the sample pools. Since the depth of the observation slots is smaller than the depth of the sample pools, the observation slots can be filled with fluid and the depth of the fluid in the observation slots is small, which is conducive to reducing the influence of the main card body, the film and the fluid itself with excessive depth on light, and the light can easily pass through the observation slots and the fluid in the observation slots. The present invention also discloses a microorganism observation system and an observation method, wherein the microorganism information acquisition device can be used to conveniently and quickly observe the changes in microorganism samples.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, and in particular to a microorganism observation test card and an observation system. Background Art

[0002] The cultivation and identification of microorganisms are of great significance for the study of microbial species and clinical medicine research. At present, there are test cards suitable for analyzing samples in automated instruments in the field of microbial detection. Automated instruments detect microorganisms by converting the information carried by light into electrical information based on the spectrum of the sample's emitted or reflected light, the intensity of light, or other characteristic readings, such as the turbidity, density, and byproducts of fluid samples. The time for automated instruments to cultivate samples and detect them is long, and results cannot be obtained quickly. However, through microscopic observation, changes in microbial samples in the fluid can be seen in a relatively short period of time, thereby obtaining results, such as whether the microorganisms reproduce, what the reproduction rate is, and whether the morphology has mutated. The microscope uses light that passes through the test card and the fluid to form an image. Obviously, the current test cards are not suitable for directly observing samples using a microscope. Summary of the invention

[0003] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides a microorganism observation test card, which is convenient for directly observing the changes of microorganism samples through a microscope.

[0004] The present invention also provides a microorganism observation system and a microorganism observation method having the microorganism observation test card.

[0005] The microbial observation test card according to the first embodiment of the present invention comprises a transparent main card body and a transparent film, wherein the end face of the main card body is provided with a fluid inlet, and the upper surface of the main card body is provided with a plurality of fluid channels, a plurality of observation slots and a plurality of sample pools, wherein the fluid channels, the observation slots and the sample pools are connected in sequence, and the depth of the observation slots is smaller than the depth of the sample pools; and the film can cover the upper surface of the main card body and seal the fluid channels, the observation slots and the sample pools.

[0006] The microbial observation test card according to the embodiment of the present invention has at least the following beneficial effects: the fluid passes through the fluid inlet, the fluid channel, and the observation slot in sequence to reach the sample pool. Since the depth of the observation slot is smaller than the depth of the sample pool, the observation slot can be filled with the fluid and the depth of the fluid in the observation slot is small, which is beneficial to reducing the influence of the fluid with too large a depth on the light. The light can easily pass through the observation slot and the fluid in the observation slot. The fluid at the observation slot can be directly observed with a microscope, and it can be observed whether there is a microbial sample in the fluid or a slight change in the microbial sample.

[0007] According to some embodiments of the present invention, a positioning protrusion is provided in the observation groove, and an upper surface of the positioning protrusion is lower than an upper surface of the main card body.

[0008] According to some embodiments of the present invention, the surface of the positioning protrusion is flat and smooth.

[0009] According to some embodiments of the present invention, the fluid channel includes at least one main channel and multiple distribution channels, one end of the main channel is connected to the fluid inlet, one end of the distribution channel is connected to the main channel, and the other end is connected to the observation groove.

[0010] According to some embodiments of the present invention, a side of the sample pool away from the observation tank is connected to a bubble collection tank.

[0011] According to some embodiments of the present invention, the sample pools are distributed in a matrix on the upper surface of the main card body.

[0012] According to some embodiments of the present invention, the number of the sample pools is 60-140.

[0013] According to some embodiments of the present invention, the cross-section of the sample pool is an oblong.

[0014] The microbial observation system according to the second embodiment of the present invention comprises a microbial information collection device and the above-mentioned microbial observation test card, wherein the microbial information collection device comprises a microscope. The test card is used to load the sample, and the changes of the microbial sample in the fluid can be known conveniently and quickly through the cooperation of the microbial information collection device and the test card.

[0015] According to the microbial observation method of the third aspect of the present invention, the above-mentioned microbial observation system is used, including the following steps: preparing a fluid containing or suspected of containing a microbial sample; filling the fluid into a test card: covering the upper surface of the test card with a film, and then connecting a catheter to the fluid inlet, placing the test card, the catheter and the fluid in a negative pressure device, and the negative pressure device sequentially fills the fluid into the catheter, the fluid channel, the observation slot and the sample pool, and fills the observation slot and the sample pool with fluid; collecting microbial image information: placing the test card carrying the fluid in an incubation device, incubating the fluid, and then placing the test card in a microbial information collection device and collecting the morphological information of the microorganism, so as to analyze whether there are microorganisms and the reproduction and growth of microorganisms. The coordinated use of the observation system and the observation method can quickly and conveniently obtain information such as whether there are microorganisms in the fluid and the reproduction and growth of microorganisms.

[0016] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0018] Figure 1 A schematic diagram of a microbial observation test card according to an embodiment of the present invention;

[0019] Figure 2 for Figure 1 A schematic cross-sectional view of a microbial observation test card is shown. DETAILED DESCRIPTION

[0020] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0021] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., and orientations or positional relationships indicated are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0022] In the description of the present invention, “several” means one or more, “more” means more than two, “greater than”, “less than”, “exceed”, etc. are understood as not including the number itself, and “above”, “below”, “within”, etc. are understood as including the number itself.

[0023] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0024] Reference Figure 1 to Figure 2 A microorganism observation test card includes a transparent main card body 100 and a transparent film. The end face of the main card body 100 is provided with a fluid inlet 200. The upper surface of the main card body 100 is provided with multiple fluid channels 300, multiple observation grooves 400 and multiple sample pools 500. The fluid channels 300, the observation grooves 400 and the sample pools 500 are connected in sequence. The depth of the observation grooves 400 is smaller than the depth of the sample pools 500. At the same time, the bottom of the observation grooves 400 is lower than the upper surface of the main card body 100; the film can cover the upper surface of the main card body 100 and seal the fluid channels 300, the observation grooves 400 and the sample pools 500.

[0025] When using the test card, a film is used to cover the upper surface of the main card body 100. The film seals the fluid channel 300, the observation slot 400 and the sample pool 500 so that the fluid inlet 200, the fluid channel 300, the observation slot 400 and the sample pool 500 form a connected network. The fluid enters the test card through the fluid inlet 200, and then fills the fluid channel 300, the observation slot 400 and the sample pool 500 in sequence. Since the depth of the observation slot 400 is smaller than that of the sample pool 500, the observation slot 400 can be filled with fluid and the depth of the fluid in the observation slot 400 is small, which is conducive to reducing the influence of the fluid with too large depth on the light. The light can easily pass through the observation slot 400 and the fluid in the observation slot 400. The fluid at the observation slot 400 can be observed with a microscope. Whether there is a microbial sample in the fluid or a slight change in the microbial sample can be observed.

[0026] See also Figure 2 , a positioning protrusion 410 is provided in the observation groove 400, and the upper surface of the positioning protrusion 410 is lower than the upper surface of the main card body 100. When observing with a microscope, the positioning protrusion 410 can be placed directly corresponding to the bottom of the objective lens to increase the speed of adjusting the position of the observed sample on the stage, and the smaller the height difference between the upper surface of the positioning protrusion 410 and the upper surface of the main card body 100, the smaller the depth of the fluid at the observation groove 400, and the more conducive it is for light to pass through the fluid. In some embodiments, the width of the observation groove 400 is equal to the width of the sample pool 500, and the contours of the observation groove 400 and the sample pool 500 form an oblong on the upper surface of the main card body 100, and the width of the positioning protrusion 410 is less than the width of the observation groove 400.

[0027] In some embodiments of the present invention, the surface of the positioning protrusion 410 is flat and smooth. The positioning protrusion 410 is columnar, and the diameter of the positioning protrusion 410 is smaller than the width of the observation slot 400. Further, the diameter of the positioning protrusion 410 can be half the width of the observation slot 400. When the observation slot 400 is opened, the positioning protrusion 410 can be opened at the same time. Of course, the positioning protrusion 410 can also be in other shapes, such as a cube.

[0028] In some embodiments of the present invention, the fluid channel 300 includes at least one main channel 310 and a plurality of distribution channels 320, one end of the main channel 310 is connected to the fluid inlet 200, one end of the distribution channel 320 is connected to the main channel 310, and the other end is connected to the observation slot 400. The fluid entering the fluid inlet 200 enters the main channel 310 and is divided into multiple branches, each branch enters a distribution channel 320, and then enters the observation slot 400 and the sample pool 500. The width of the main channel 310 is larger than the width of the distribution channel 320, so that the main channel 310 has a greater ability to transport fluid than the distribution channel 320, thereby meeting the needs of multiple distribution channels 320 and improving the speed of filling the test card with fluid. In some embodiments, the sample pool 500 is divided into two large groups, front and back, and one main channel 310 is provided with two, and the two main channels 310 correspond to two groups of sample pools 500, respectively, to further improve the speed of transporting fluid into the sample pool 500.

[0029] In some embodiments of the present invention, the side of the sample pool 500 away from the observation slot 400 is connected to a bubble collecting slot 600. When filling the fluid, the fluid inlet 200 is connected to the conduit to extract the air in the test card, and then the free end of the conduit is immersed in the fluid test tube. Finally, the fluid in the test tube is filled into the test card under the action of external air pressure. During this process, the residual gas in the fluid channel 300, the observation slot 400 and the sample pool 500 will be collected in the bubble collecting slot 600 to avoid bubbles in the observation slot 400. In some embodiments, the bubble collecting slot 600 is ovoid in shape.

[0030] In some embodiments of the present invention, the sample pools 500 are arranged in a matrix on the upper surface of the main card body 100. The orderly arrangement of the sample pools 500 is conducive to marking or distinguishing each sample pool 500 and is aesthetically pleasing. The number of sample pools 500 is 60-140. A large amount of sample data can be obtained by cultivating or observing samples at one time, without using multiple test cards, thus reducing the workload of personnel. Figure 1 In this embodiment, the number of sample pools 500 is eight rows by twelve columns.

[0031] In some embodiments of the present invention, the main card body 100 is a cube with a certain shape and specifications, and is easy to take. A transition surface is provided between two adjacent end surfaces of the main card body 100 to facilitate identification of the upper and lower surfaces of the main card body 100. The main card body 100 and the film are both made of transparent materials and have good light transmittance.

[0032] In some embodiments of the present invention, the cross-section of the sample pool 500 is an oblong, and the length direction of the oblong is consistent with the arrangement direction of each row of sample pools 500, so that the arrangement of the sample pools 500 is more orderly and compact, and the space of the test card can be fully utilized.

[0033] The present invention also provides a microorganism observation system, which includes a microorganism information collection device and a microorganism observation test card in any of the above embodiments, and the microorganism information collection device includes a microscope. The fluid sample is filled in the test card, the test card is placed on the microscope stage, and the microscope is adjusted, and people can observe the changes in the sample through the microscope. The microorganism information collection device also includes an image analysis system, which analyzes the collected fluid image information to determine whether there are microorganisms in the fluid, whether the microorganisms reproduce, what the reproduction speed is, whether the morphology has changed, etc.

[0034] The present invention also provides a microorganism observation method, which uses the above-mentioned microorganism observation system, including the following steps: preparing a fluid containing or suspected of containing a microorganism sample; filling the fluid into a test card: covering the upper surface of the test card with a film, then connecting a catheter to the fluid inlet 200, placing the test card, the catheter and the fluid in a negative pressure device, and the negative pressure device sequentially fills the fluid into the catheter, the fluid channel 300, the observation slot 400 and the sample pool 500, and fills the observation slot 400 and the sample pool 500 with fluid; collecting microorganism image information: placing the test card carrying the fluid in an incubation device, incubating the fluid, and then placing the test card in a microorganism information collection device and collecting microorganism morphology information, so as to analyze whether there are microorganisms and the growth of microorganisms. The coordinated use of the observation system and the observation method can quickly and conveniently obtain information such as whether there are microorganisms in the fluid and the reproduction and growth of microorganisms.

[0035] In some embodiments, the fluid is filled into the test card by the following operating steps: a film is covered on the upper surface of the test card, a catheter is connected to the fluid inlet 200, the test card, the catheter and the fluid are placed in the negative pressure chamber of the negative pressure device, and the free end of the catheter is extended into a beaker or test tube containing the fluid, the negative pressure chamber is evacuated, and the air in the fluid inlet 200, the fluid channel 300, the observation slot 400 and the sample pool 500 is evacuated, so that a negative pressure is formed inside the test card, and then the negative pressure chamber returns to the atmospheric pressure state, and the fluid can be squeezed into the test card by the atmospheric pressure.

[0036] After the microbial information collection device collects the image information of the fluid, it can be directly presented to people for direct observation, and the image analysis system can also be used to analyze and determine the growth and reproduction of the microorganisms and their morphology.

[0037] The embodiments of the present invention are described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Various changes can be made within the knowledge scope of ordinary technicians in the technical field without departing from the purpose of the present invention.

Claims

1. A microbial observation test card, characterized in that: include: A transparent main card body, wherein the end face of the main card body is provided with a fluid introduction port, the upper surface of the main card body is provided with a plurality of fluid channels, a plurality of observation slots and a plurality of sample pools, the fluid channels, the observation slots and the sample pools are connected in sequence, the depth of the observation slots is smaller than the depth of the sample pools, a positioning protrusion is provided in the observation slots, the upper surface of the positioning protrusions is lower than the upper surface of the main card body, the surface of the positioning protrusions is flat and smooth, the side of the sample pools away from the observation slots is connected with a bubble collection slot, and the cross section of the sample pools is an oblong; A transparent film can cover the upper surface of the main card body and seal the fluid channel, the observation slot and the sample pool.

2. The microbial observation test card according to claim 1, characterized in that: The fluid channel includes at least one main channel and a plurality of distribution channels, one end of the main channel is connected to the fluid inlet, one end of the distribution channel is connected to the main channel, and the other end is connected to the observation groove.

3. The microbial observation test card according to claim 1, characterized in that: The sample pools are distributed in a matrix on the upper surface of the main card body.

4. The microbial observation test card according to claim 1 or 3, characterized in that: The number of the sample pools is 60-140.

5. A microbial observation system, characterized in that: It comprises a microorganism information collection device and the microorganism observation test card according to any one of claims 1 to 4, wherein the microorganism information collection device comprises a microscope.

6. A method for observing microorganisms, characterized in that: Using the microorganism observation system according to claim 5, The following steps are included: Prepare fluid samples containing or suspected of containing microorganisms; Filling the test card with fluid: Covering the upper surface of the test card with a film, then connecting a catheter to the fluid inlet, placing the test card, the catheter and the fluid in a negative pressure device, and the negative pressure device sequentially fills the fluid into the catheter, the fluid channel, the observation slot and the sample pool, and fills the observation slot and the sample pool with the fluid; Collecting microbial image information: placing the test card carrying the fluid in an incubation device to incubate the fluid, then placing the test card in a microbial information collection device and collecting microbial morphological information to analyze whether there are microorganisms and the reproduction and growth of microorganisms.

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