A giant panda feces sampler

Abstract

The present invention provides a giant panda feces sampler, including a sampler housing assembly, a sampling barrel assembly, a sampling linkage device, a sampling push rod assembly and a sampler strut sliding sleeve assembly; the sampling barrel assembly is installed in the sampler housing assembly through the sampling linkage device, a sampling push rod assembly is provided on the top of the sampling barrel assembly, and a sampler strut sliding sleeve assembly is provided in the bottom of the sampling barrel assembly. The present invention has strong pertinence, novel structure, mechanical linkage, and simple operation, and can achieve dirt layer separation and aseptic collection. The sampling nozzle can be switched on and off, and before formally collecting the sample, the pollutants attached to the feces surface can be peeled off through the sampling nozzle before the sample is collected, so as to ensure to the greatest extent that the collected sample is derived from the intestinal tract of the giant panda, and ensure the authenticity of the research results.

Description

Technical Field

[0001] The invention belongs to the technical field of intestinal microorganisms, and particularly relates to a giant panda feces sampler. Background Art

[0002] The giant panda is a rare and endangered animal unique to my country and a flagship animal for wildlife protection in the world. Its number is very small and it mainly lives in the mountainous area at the junction of Sichuan, Shaanxi and Gansu provinces in my country. It belongs to the class of mammals and the order of carnivora. In order to adapt to the living environment, the giant panda has specialized in eating bamboo, but the giant panda's intestines still retain the digestive system of typical carnivorous mammals. In the long-term evolution process, its intestinal flora has formed a system of mutual dependence and mutual restraint between different species in the flora, between the flora and the host, and between the flora, the host and the surrounding environment through individual adaptation and natural selection. Therefore, studying the intestinal microbial flora and structure of giant pandas is of great significance to understanding the nutrition, digestion and species evolution of giant pandas.

[0003] At present, most of the research on intestinal microorganisms is carried out on feces. The collection of fecal samples is a convenient and non-invasive method with the characteristics of non-invasive and convenient sampling. Fecal flora is often used to represent intestinal flora in research. Therefore, collecting feces and studying its microbial flora provides an excellent sample and valuable source for understanding the structure of intestinal microbial flora of rare national treasures such as giant pandas, exploring related strain resources, and developing microbial products. However, after feces are discharged from the giant panda, they are easily affected by the environment, such as feces, weeds, soil and other existing microorganisms in the environment. In order to ensure the rigor and scientificity of the experiment, when collecting fecal samples, it is necessary not only to ensure sterile collection, but also to prevent interference from external environmental microorganisms, so as to minimize the impact and interference of external environmental microorganisms on the final research results.

[0004] However, due to differences in research purposes, current giant panda feces collection devices almost do not take into account the problem of microbial contamination in the external environment, and have defects such as rough collection methods and poor reliability of collected samples. It is very necessary to design a giant panda feces sampler for the study of giant panda intestinal microbial flora that can meet the research needs of dirt layer separation and sterile collection, while also being simple and convenient to operate. Summary of the invention

[0005] In view of the defects of the prior art, the present invention provides a giant panda feces sampler, which can effectively solve the problems existing in the above-mentioned prior art.

[0006] In order to achieve the above invention object, the technical solution adopted by the present invention is as follows:

[0007] A giant panda feces sampler comprises a sampler housing component, a sampling tube component, a sampling linkage device, a sampling push rod component and a sampler support rod sliding sleeve component;

[0008] The sampling tube assembly is installed in the sampler housing assembly through a sampling linkage device. A sampling push rod assembly is arranged on the top of the sampling tube assembly, and a sampler support rod sliding sleeve assembly is arranged in the bottom of the sampling tube assembly.

[0009] Specifically, the sampler housing assembly includes a shell and a sample spreader;

[0010] The housing is a stainless steel rectangular tube, and the housing includes an outer shell, an outer shell end cover wheel disc and an end cover are installed on the upper end of the housing and fixed by the end cover fixing screws, a circular hole is provided in the center of the outer shell end cover wheel disc and the end cover, a first buckle claw seat and a second buckle claw seat are symmetrically installed on the outer side of the middle part of the housing, and a first buckle release button and a second release button are symmetrically installed at the bottom of the first buckle claw seat and the second buckle claw seat, and the upper ends of the first hinge and the second hinge are symmetrically fixed to the two sides of the lower end of the housing by the first hinge fixing screw and the second hinge fixing screw;

[0011] The lower end of the sample spreader is a first sampling nozzle and a second sampling nozzle, and the first sampling nozzle and the second sampling nozzle are connected to the housing through a first hinge and a second hinge respectively;

[0012] The sampling tube assembly comprises: a sampling tube handle, a sampling tube, a sampling tube positioning sleeve, and a bushing; the sampling tube handle is connected to the sampling tube; the sampling tube positioning sleeve is fixed on the sampling tube; and the lower end of the sampling tube is a sample collection port.

[0013] The sampling linkage device comprises: a sampling nozzle positioning locking plate, a bushing, and a spring compression sleeve;

[0014] The bushing is sleeved outside the sampling tube, the sampling nozzle positioning locking plate is located in the upper middle part of the bushing, and the lower end of the bushing is connected to the spring compression sleeve;

[0015] When the sampler is working, the sampling nozzle positioning locking plate is locked by the first locking claw and the second locking claw;

[0016] The first release button and the second release button are located on the shell. After sampling is completed, the first release button and the second release button are pressed, and the sampling nozzle positioning locking plate is disengaged from the first catch pin and the second catch pin under the linkage action of the first catch pin and the second catch spring and the second catch spring.

[0017] The sampling push rod assembly includes: a push rod handle, a push rod, a push rod positioning sleeve, and a sampling port sample pushing slider; the push rod handle is connected to the push rod; the push rod positioning sleeve is fixed on the push rod; a push rod spring compression sleeve is provided outside the push rod and the push rod return spring; and the sampling port sample pushing slider is fixed to the lower end of the push rod.

[0018] The sampler support rod sliding sleeve assembly comprises: a sampling nozzle closing spring stop sleeve, a sampling nozzle closing spring, a supporting connecting rod sliding sleeve seat, an O-shaped shaft pin groove, and a supporting connecting rod sliding sleeve;

[0019] The sampling nozzle closing spring stop sleeve is connected to the supporting connecting rod sliding sleeve and is fixed to the housing through the first supporting connecting rod sliding sleeve limit screw and the second supporting connecting rod sliding sleeve limit screw. The supporting connecting rod sliding sleeve seat is located below the sampler support rod sliding sleeve and is fixed to the lower end of the housing; the sampling nozzle closing spring sleeve is sleeved on the supporting connecting rod sliding sleeve;

[0020] The O-shaped shaft pin groove is located at the lower end of the sampler support rod sliding sleeve, and is connected to the first support nozzle connecting rod and the second support nozzle connecting rod respectively through the O-shaped shaft pin groove; the first support nozzle connecting rod is hinged on the inner wall of the first sampling nozzle; the second support nozzle connecting rod is hinged on the inner wall of the second sampling nozzle.

[0021] The present invention provides a giant panda feces sampler, which has strong pertinence, novel structure, mechanical linkage, simple operation, and can achieve separation of dirt layers and aseptic collection. The present invention mainly designs a sampler for the study of giant panda intestinal microbial flora. All components of the present invention are made of metal and can withstand high temperature and high pressure sterilization. The samples collected by the sterilized sampler can meet the needs of aseptic collection of samples. In addition, the present invention is designed with a sampling nozzle that can be switched on and off. Before formally collecting the sample, the pollutants attached to the surface of the feces can be peeled off through the sampling nozzle before the sample is collected, so as to ensure to the greatest extent that the collected samples are derived from the giant panda intestine and ensure the authenticity of the research results. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1A is a schematic diagram of the structure of the sampler in a closed state according to an embodiment of the present invention;

[0023] Figure 1B It is a structural schematic diagram of the working state of the sampler according to an embodiment of the present invention;

[0024] Figure 2 is a schematic structural diagram of a housing according to an embodiment of the present invention;

[0025] Figure 3 It is a schematic diagram of the structure of a sampling tube according to an embodiment of the present invention;

[0026] Figure 4A It is a structural schematic diagram of the sampling linkage device in a resting state according to an embodiment of the present invention;

[0027] Figure 4B It is a structural schematic diagram of the working state of the sampling linkage device according to an embodiment of the present invention;

[0028] Figure 5 is a schematic structural diagram of a sampling push rod according to an embodiment of the present invention;

[0029] Figure 6 It is a schematic structural diagram of a sampler support rod sliding sleeve according to an embodiment of the present invention. DETAILED DESCRIPTION

[0030] The specific technical solution of the present invention is explained in conjunction with embodiments.

[0031] like Figure 1A , 1B and Figure 2 As shown, a giant panda feces sampler includes a sampler housing assembly, a sampling tube assembly, a sampling linkage device, a sampling push rod assembly and a sampler support rod sliding sleeve assembly;

[0032] The sampler housing assembly includes a shell A and a sample spreader B.

[0033] The shell A is a stainless steel rectangular tube, and includes a shell 18. The shell end cover wheel 11 and the end cover 13 are installed on the upper end of the shell 18 and fixed by the end cover fixing screws 12. The shell end cover wheel 11 and the end cover 13 are provided with circular holes in the centers. The first claw seat 21 and the second claw seat 50 are symmetrically installed on the outer side of the middle part of the shell A. The first claw seat 21 and the second claw seat 50 are respectively fixed by the first claw seat fixing screws 20 and the second claw seat fixing screws 25. The first claw seat 21 and the second claw seat 50 are also symmetrically installed with the first release button 51 and the second release button 24 at the bottom. The first hinge 35 and the second hinge 57 are symmetrically installed on both sides of the lower end of the shell A. The upper ends of the first hinge 35 and the second hinge 57 are symmetrically fixed to both sides of the lower end of the shell A by the first hinge fixing screws 34 and the second hinge fixing screws 56 respectively.

[0034] The sample opener B is made of metal material, and the lower end of the sample opener B is a first sampling nozzle 44 and a second sampling nozzle 64. The first supporting connecting rod seat rivet 43 and the second supporting connecting rod seat rivet 63 are symmetrically installed on both sides of the middle of the sample opener B. The lower ends of the first hinge 35 and the second hinge 57 are fixed to the upper end of the sample opener B by the third hinge fixing screw 37 and the fourth hinge fixing screw 58 respectively. The sample opener B is connected to the shell A as a whole.

[0035] like Figure 3 As shown, the sampling cylinder assembly includes: a sampling cylinder handle 5, a sampling cylinder 10, a sampling cylinder positioning sleeve 15, and a bushing 17; the sampling cylinder handle 5 is connected to the sampling cylinder 10 through the sampling cylinder handle fixing screw 7; the sampling cylinder positioning sleeve 15 is fixed to the sampling cylinder 10 through the first sampling cylinder positioning sleeve fixing screw 14 and the second sampling cylinder positioning sleeve fixing screw 45; the lower end of the sampling cylinder 10 is a sample collection port 59.

[0036] like Figure 4A and Figure 4BAs shown, the sampling linkage device includes: a sampling nozzle positioning locking plate 16, a bushing 17, a spring compression sleeve 26, a first buckle claw 19 and a second buckle claw 46, a fourth buckle claw seat fixing screw 52 and a third buckle claw seat fixing screw 47, a first buckle grasping shaft pin 22 and a second buckle grasping shaft pin 48, a first buckle claw spring 23 and a second buckle claw spring 49, a first release button 24 and a second release button 51;

[0037] The bushing 17 is sleeved on the outside of the sampling tube 10 , the sampling nozzle positioning locking plate 16 is located in the upper middle part of the bushing 17 , and the lower end of the bushing 17 is connected to the spring compression sleeve 26 .

[0038] When the sampler is working, the sampling nozzle positioning locking plate 16 is locked by the first locking claw 19 and the second locking claw 46 .

[0039] The first release button 24 and the second release button 51 are located on the shell A. After sampling is completed, the first release button 24 and the second release button 51 are pressed, and the sampling nozzle positioning locking plate 16 is disengaged from the first catch pin 22 and the second catch pin 48 and the first catch spring 23 and the second catch spring 49 under the linkage action.

[0040] like Figure 5 As shown, the sampling push rod assembly includes: a push rod handle 1, a push rod 4, a push rod positioning sleeve 8, and a sampling port sample pushing slider 32;

[0041] The push rod handle 1 is connected to the push rod 4 through the handle fixing screw 2;

[0042] The push rod positioning sleeve 8 is fixed on the push rod 4 by the push rod positioning sleeve fixing screw 9; the push rod 4 and the push rod return spring 6 are provided with a push rod spring compression sleeve 3;

[0043] The sampling port sample pushing slider 32 is fixed to the lower end of the push rod 4 by the sample pushing slider fixing screw 33.

[0044] like Figure 6 As shown, the sampler support rod sliding sleeve assembly includes: a sampling nozzle closing spring stop sleeve 28, a sampling nozzle closing spring 31, a supporting connecting rod sliding sleeve seat 36, an O-shaped shaft pin groove 39, and a supporting connecting rod sliding sleeve 55;

[0045] The sampling nozzle closing spring stop sleeve 28 is connected to the supporting connecting rod sliding sleeve 55, and is fixed to the shell A by the first supporting connecting rod sliding sleeve limiting screw 29 and the second supporting connecting rod sliding sleeve limiting screw 53. The first supporting connecting rod sliding sleeve limiting screw 29 and the second supporting connecting rod sliding sleeve limiting screw 53 are respectively located in the first limiting sliding groove 30 and the second limiting sliding groove 54 of the shell A.

[0046] The supporting connecting rod sliding sleeve 36 is located below the sampler support rod sliding sleeve 55 and is fixed to the lower end of the housing A by a first hinge fixing screw 34 and a second hinge fixing screw 56 .

[0047] The sampling nozzle closing spring 31 is sleeved on the supporting connecting rod sliding sleeve 55 .

[0048] The O-shaped axle pin groove 39 is located at the lower end of the sampler support rod sliding sleeve 55, and is respectively connected to the first support nozzle connecting rod 40 and the second support nozzle connecting rod 60 through the first support connecting rod O-shaped axle pin 38 and the second support connecting rod O-shaped axle pin 65 located on the O-shaped axle pin groove 39; the first support nozzle connecting rod 40 is hinged to the inner wall of the first sampling nozzle 44 through the first support connecting rod axle pin 41 and the first connecting rod seat 42; the second support nozzle connecting rod 60 is hinged to the inner wall of the second sampling nozzle 64 through the second support connecting rod seat 61 and the support connecting rod axle pin 62.

[0049] Working principle: When sampling panda feces, first insert the first sampling nozzle 44 and the second sampling nozzle 64 of the sampler under the surface of the feces, hold the housing A with the left hand, and push the sampling tube handle 5 with the right hand at a constant speed. Under the push of the sampling tube handle 5, the positioning sleeve 15, the sampling nozzle positioning locking plate 16, the bushing 17, the spring compression sleeve 26 and the sampling nozzle opening spring 27 on the sampling tube are pushed in sequence. First, after the sampling nozzle positioning locking disk 16 is pushed into the first claw 19 and the second claw 46 and locked, the sampling nozzle opening spring 27 is compressed under the action of the spring compression sleeve 26. The elastic force generated after compression pushes the supporting connecting rod sliding sleeve 55 to move forward, and the sampling nozzle closing spring stop sleeve 28 fixed on the supporting connecting rod sliding sleeve 55 moves accordingly. The first supporting nozzle connecting rod 40 and the second supporting nozzle connecting rod 60 installed at the end of the supporting connecting rod sliding sleeve 55 are also pushed respectively, and the first sampling nozzle 44 and the second sampling nozzle 64 are gradually opened. At this time, the contaminated part of the surface of the feces is peeled off by the first sampling nozzle 44 and the second sampling nozzle 64.

[0050] In order to prevent the first sampling nozzle 44 and the second sampling nozzle 64 of the sampler from being damaged and affecting the normal operation of the sampler, the present invention specifically designs the first support link sliding sleeve limit screw 29 and the second support link sliding sleeve limit screw 53 on the sampling nozzle closing spring stop sleeve 28. When the support link sliding sleeve 55 is pushed, the first support link sliding sleeve limit screw 29 and the second support link sliding sleeve limit screw 53 located on the sampling nozzle closing spring stop sleeve 28 are immediately pushed to the end of the first limit slide groove 30 and the second limit slide groove 54. Since the support link sliding sleeve 55 cannot be pushed forward any further, the opening width of the first sampling nozzle 44 and the second sampling nozzle 64 is controlled within a set range to ensure that the first sampling nozzle 44 and the second sampling nozzle 64 will not be damaged.

[0051] When the sampling nozzle positioning locking plate 16 is locked, the first sampling nozzle 44 and the second sampling nozzle 64 are fully opened to push away the feces on the sample surface, and sample collection can begin. According to the state of the sample on site, the sampling cylinder 10 is pulled forward and backward, or the sampling cylinder 10 can be rotated left and right to collect the panda feces sample. After the collection is completed, the sterile centrifuge tube for collecting the sample is placed at the collection port 59 at the lower end of the sampling cylinder 10, and the push rod handle 1 is pushed to push the sample collected in the sampling cylinder 10 into the centrifuge tube, and the centrifuge tube is covered to complete the sample collection. Immediately release the push rod handle 1, and the push rod handle 1 returns to its original position under the action of the push rod return spring 6.

[0052] When the supporting link sliding sleeve 55 is pushed forward, the sampling nozzle closing spring 31 begins to store energy after being compressed by the supporting link sliding sleeve 55. The stored energy is used when the first sampling nozzle 44 and the second sampling nozzle 64 are closed. When the sampling is completed, the first release button 24 and the second release button 51 are pressed at the same time, the first catch 19 and the second catch 46 are released from the sampling nozzle positioning locking plate 16, and the sampling nozzle opening spring 27 and the sampling nozzle closing spring 31 are immediately released, and the first supporting nozzle connecting rod 40 and the second supporting nozzle connecting rod 60 at the end of the supporting link sliding sleeve 55 are retracted, and the first sampling nozzle 44 and the second sampling nozzle 64 are immediately closed, and the sampler returns to the state of being closed. Figure 1A status.

Claims

1. A giant panda feces sampler, characterized in that: It includes a sampler housing assembly, a sampling tube assembly, a sampling linkage device, a sampling push rod assembly and a sampler support rod sliding sleeve assembly; The sampling tube assembly is installed in the sampler housing assembly through a sampling linkage device, a sampling push rod assembly is provided on the top of the sampling tube assembly, and a sampler support rod sliding sleeve assembly is provided in the bottom of the sampling tube assembly; The sampler housing assembly comprises a housing A and a sample spreader B; The housing A is a stainless steel rectangular tube, and the housing A includes a shell (18). The upper end of the shell (18) is mounted with a shell end cover wheel (11) and an end cover (13) and fixed by an end cover fixing screw (12). The center of the shell end cover wheel (11) and the end cover (13) is provided with a circular hole. The first claw seat (21) and the second claw seat (50) are symmetrically mounted on the outer side of the middle part of the housing A. The bottom of the first claw seat (21) and the second claw seat (50) are also symmetrically mounted with a first release button (24) and a second release button (51). The upper ends of the first hinge (35) and the second hinge (57) are symmetrically fixed to the two sides of the lower end of the housing A by a first hinge fixing screw (34) and a second hinge fixing screw (56). The sample spreader B is made of metal material, and the lower end of the sample spreader B is a first sampling nozzle (44) and a second sampling nozzle (64), and the first sampling nozzle (44) and the second sampling nozzle (64) are connected to the housing A via a first hinge (35) and a second hinge (57) respectively; The sampling barrel assembly comprises: a sampling barrel handle (5), a sampling barrel (10), a sampling barrel positioning sleeve (15), and a bushing (17); the sampling barrel handle (5) is connected to the sampling barrel (10); the sampling barrel positioning sleeve (15) is fixed on the sampling barrel (10); the lower end of the sampling barrel (10) is a sample collection port (59); The sampling linkage device comprises: a sampling nozzle positioning locking plate (16), a bushing (17), and a spring compression sleeve (26); The bushing (17) is sleeved on the outside of the sampling tube (10), the sampling nozzle positioning locking plate (16) is located in the upper middle part of the bushing (17), and the lower end of the bushing (17) is connected to the spring compression sleeve (26); When the sampler is working, the sampling nozzle positioning locking plate (16) is locked by the first locking claw (19) and the second locking claw (46); The first release button (24) and the second release button (51) are located on the housing A. After sampling is completed, the first release button (24) and the second release button (51) are pressed, and the sampling nozzle positioning locking plate (16) is released from the first catch (19) and the second catch (46) under the linkage action of the first catch shaft pin (22) and the second catch shaft pin (48) and the first catch spring (23) and the second catch spring (49); The sampling push rod assembly comprises: a push rod handle (1), a push rod (4), a push rod positioning sleeve (8), and a sampling port sample pushing slider (32); the push rod handle (1) is connected to the push rod (4); the push rod positioning sleeve (8) is fixed to the push rod (4); a push rod spring compression sleeve (3) is provided outside the push rod (4) and the push rod return spring (6); the sampling port sample pushing slider (32) is fixed to the lower end of the push rod (4); The sampler support rod sliding sleeve assembly comprises: a sampling nozzle closing spring stop sleeve (28), a sampling nozzle closing spring (31), a supporting connecting rod sliding sleeve seat (36), an O-shaped shaft pin groove (39), and a supporting connecting rod sliding sleeve (55); The sampling nozzle closing spring stop sleeve (28) is connected to the supporting connecting rod sliding sleeve (55) and is fixed to the housing A through the first supporting connecting rod sliding sleeve limit screw (29) and the second supporting connecting rod sliding sleeve limit screw (53); the supporting connecting rod sliding sleeve seat (36) is located below the supporting connecting rod sliding sleeve (55) and is fixed to the lower end of the housing A; the sampling nozzle closing spring (31) is sleeved on the supporting connecting rod sliding sleeve (55); The O-shaped shaft pin groove (39) is located at the lowermost end of the support connecting rod sliding sleeve (55), and is respectively connected to the first support nozzle connecting rod (40) and the second support nozzle connecting rod (60) through the O-shaped shaft pin groove (39); the first support nozzle connecting rod (40) is hinged on the inner wall of the first sampling nozzle (44); and the second support nozzle connecting rod (60) is hinged on the inner wall of the second sampling nozzle (64).

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