A sample addition indicator strip and an incubation plate with the sample addition indicator strip

Abstract

The present invention relates to a sample addition indicator strip, comprising: a bottom strip configured to be adapted to be placed on a carrying device having a plurality of liquid addition grooves; and a plurality of indicator holes provided on the bottom strip, wherein the positions of the plurality of indicator holes correspond to the plurality of liquid addition grooves of the carrying device; wherein, the plurality of indicator holes include an indicating material adapted to indicate whether it contacts the liquid added to the liquid addition groove. This application greatly reduces the phenomena of missed addition and misaddition during the sample addition process, reduces the error probability during the sample addition process, and ensures accurate experimental results. At the same time, it has high operability, can improve the experimental efficiency, save time, and reduce experimental losses.

Description

Technical Field

[0001] The present invention relates to an experimental consumable, and particularly to a sample addition indicator strip and an incubation plate with the sample addition indicator strip. Background Art

[0002] Blotting experiments are usually carried out in an incubation plate. The incubation plates are divided into 30-well / incubation plate, 44-well / incubation plate, 48-well / incubation plate and 50-well / incubation plate according to different specifications. Due to the large number of wells, it is easy to miss or mis-add samples during the sequential sample addition process.

[0003] Fully ensuring the correctness of the sample addition process is the most effective method to ensure accurate test results and reduce experimental losses, and is also the most direct manifestation of being responsible for patients. Currently, laboratories often use a marker pen to mark sample numbers or component codes on the incubation plate to avoid errors. Although this method can achieve a certain effect of avoiding errors, in the case of a huge sample volume, this method has low operability and low efficiency, and it is also inevitable to mis-add or miss samples or components. Summary of the Invention

[0004] In view of the technical problems existing in the prior art, the present invention provides a sample addition indicator strip, including: a bottom strip configured to be placed on a carrying device having a plurality of liquid addition grooves; and a plurality of indicator holes provided on the bottom strip, wherein the positions of the plurality of indicator holes correspond to the plurality of liquid addition grooves of the carrying device; and an indicating material is included in the plurality of indicator holes, which is adapted to indicate whether it contacts with the liquid added to the liquid addition groove.

[0005] The sample addition indicator strip as described above further includes a waterproof layer covering the bottom strip.

[0006] The sample addition indicator strip as described above, wherein the indicating material is anhydrous cobalt chloride.

[0007] The sample addition indicator strip as described above, wherein an anti-pollution material is included above the plurality of indicator holes.

[0008] The sample addition indicator strip as described above, wherein the anti-pollution material is cotton fiber or filter paper fiber.

[0009] The sample addition indicator strip as described above, wherein the side of the bottom strip without indicator holes includes an adhesive.

[0010] According to another aspect of the present invention, a roll of sample addition indicator strips is provided, including any one of the above-mentioned sample addition indicator strips.

[0011] According to another aspect of the present invention, a incubation tray is provided, including: a tray body; a plurality of liquid adding grooves disposed on the tray body; and a sample adding indicator strip as described in any one of the above; wherein, the positions of the plurality of indicating holes of the sample adding indicator strip as described in any one of the above correspond to the plurality of liquid adding grooves.

[0012] According to another aspect of the present invention, a method for using a sample adding indicator strip is provided, including: intercepting a part of the sample adding indicator strip as described in any one of the above; and pasting the intercepted part of the sample adding indicator strip as described in any one of the above on the incubation tray, such that the positions of the plurality of indicating holes of the sample adding indicator strip as described in any one of the above correspond to the plurality of liquid adding grooves of the incubation tray.

[0013] According to another aspect of the present invention, a method for adding liquid using the sample adding indicator strip as described in any one of the above is provided, including: before or after adding liquid into the liquid adding groove, contacting the adding liquid head for adding liquid with the indicating hole of the sample adding indicator strip as described in any one of the above corresponding to the liquid adding groove.

[0014] According to another aspect of the present invention, a method for adding liquid using the incubation tray as described above is provided, including: before or after adding liquid into the liquid adding groove, contacting the adding liquid head for adding liquid with the indicating hole of the sample adding indicator strip as described in any one of the above corresponding to the liquid adding groove.

[0015] This application greatly reduces the phenomena of missed addition and wrong addition during the sample adding process, reduces the error probability during the sample adding process, and ensures the accuracy of the experimental results. At the same time, it has high operability, can improve the experimental efficiency, save time, and reduce experimental losses. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Next, the preferred embodiments of the present invention will be further described in detail with reference to the drawings, wherein:

[0017] Figure 1A is the front view of the sample adding indicator strip according to an embodiment of the present invention;

[0018] Figure 1B is the top view of the sample adding indicator strip according to an embodiment of the present invention;

[0019] Figure 1C is the partial enlarged view of the sample adding indicator strip according to an embodiment of the present invention;

[0020] Figure 2 is the roll of the sample adding indicator strip according to an embodiment of the present invention; and

[0021] Figure 3 is the schematic diagram of the use of the sample adding indicator strip on the incubation tray according to an embodiment of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Apparently, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0023] In the following detailed description, reference may be made to the accompanying drawings that form a part hereof, and in which are shown by way of illustration specific embodiments in which the application may be practiced. In the drawings, like reference numerals describe substantially similar components in different figures. The various specific embodiments of the application have been described in sufficient detail below to enable those of ordinary skill in the art with relevant knowledge and technology to implement the technical solutions of the application. It should be understood that other embodiments may be utilized or structural, logical, or electrical changes may be made to the embodiments of the application. In the application, the same reference numerals represent the same structure.

[0024] The present application provides a sample addition indicator strip, which can be used for a incubation tray to perform experiments such as immunoassay. It has a simple structure, strong operability, can effectively improve the experimental efficiency, and save experimental consumables.

[0025] Figures 1A - 1C Fig. shows a sample addition indicator strip according to an embodiment of the present application; wherein, Figure 1A is a schematic structural view of the sample addition indicator strip, Figure 1B is a schematic structural view of the sample addition indicator strip from another angle, Figure 1C is a partial enlarged view of the sample addition indicator strip. As Figures 1A - 1C shown, the sample addition indicator strip 100 (sometimes also referred to as the indicator strip herein, and the two represent the same meaning) includes: a bottom strip 110, a plurality of indicator holes 121 - 128, and an indicator material 130. The bottom strip 110 is adapted to be placed on a carrying device having a plurality of liquid addition grooves, such as an incubation tray. The plurality of indicator holes 121 - 128 are provided on the bottom strip 110, and their positions correspond one-to-one with the liquid addition grooves on the carrying device. The plurality of indicator holes 121 - 128 include the indicator material 130, which is adapted to indicate whether it is in contact with the liquid added to the liquid addition groove, and further indicate whether the reagent addition in a certain liquid addition groove has been completed.

[0026] As Figure 1A and Figure 1B shown, the bottom strip 110 is in a strip shape. The surface of the bottom strip 110 can withstand a certain degree of water molecule invasion, so that it has a stable shape and will not be deformed due to being contaminated by liquid reagents, and has good durability, so as to extend its storage period and service life to the greatest extent. In some embodiments, the bottom strip 110 has good flexibility and can be placed in a roll (refer to Figure 2) for convenient storage. In some embodiments, the bottom strip 110 is made of a waterproof material, such as waterproof paper. In some embodiments, the bottom strip 110 is attached with a waterproof layer 160. In some embodiments, the waterproof layer 160 is a film, which has good flexibility and waterproofness to prevent the bottom strip 110 and the indicating material 130 from getting damp.

[0027] In some embodiments, the width H of the bottom strip 110 is 3 - 7 mm. For example, the width H of the bottom strip 110 can be about 5 mm to facilitate its matching use with the carrying device.

[0028] As Figure 1A and Figure 1B shown, the indicating holes 121 - 128 are provided on the bottom strip 110 and have a concave structure. In some embodiments, the indicating holes 121 - 128 are formed by the bottom strip 110 sinking downward. In some embodiments, the indicating holes 121 - 128 are formed by extruding the bottom strip 110 with a hard object. In some embodiments, the indicating holes 121 - 128 are formed by etching on the bottom strip 110. In some other embodiments, the indicating holes 121 - 128 can also be horizontal or convex structures. Without limitation, the concave structure is more conducive to storing the indicating material and is a more preferred solution.

[0029] The indicating holes 121 - 128 are arranged along the long side direction of the bottom strip 110, and the centers of the multiple indicating holes 121 - 128 are arranged in a straight line, and their center connection line is parallel to the long side of the bottom strip 110, making the indicating strip 100 both beautiful and capable of being cut according to the size of the equipment used in combination with it. In some embodiments, the distances from the indicating holes 121 - 128 to the two sides of the wide side of the bottom strip 110 are equal.

[0030] In some embodiments, the indicating holes 121 - 128 are dot grooves. The distance L between adjacent indicating holes is equal, about 7 - 12 mm, and its distance is the same as the distance between adjacent liquid adding grooves on the carrying device. In some embodiments, the distance L between adjacent indicating holes is about 9 mm.

[0031] In some embodiments, the diameter of the indicating holes 121 - 128 is about 1.0 - 3.0 mm. For example, the diameter of the indicating holes 121 - 128 can be about 1.5 mm. The size of the indicating holes 121 - 128 is convenient for the experimenter to touch with the pipette tip of the pipette gun 300 and can accommodate a small amount of liquid reagent. The diameter and depth of the indicating holes 121 - 128 are not limited in this experiment.

[0032] In some embodiments, an indicating material 130 is included in the grooves of the indicating holes 121 - 128. By the change in the properties of the indicating material, the reagent addition situation in the corresponding liquid adding tank is characterized. In some embodiments, the indicating material 130 is anhydrous cobalt chloride. Anhydrous cobalt chloride is blue, hygroscopic, and turns pink or red after absorbing moisture. Its color change is rapid, which can promptly indicate whether a reagent is added to the corresponding liquid adding tank on the carrying device. In some embodiments, the indicating material 130 is added on the surface of the waterproof layer 160 inside the indicating hole to prevent the indicating material 130 from being affected by moisture and denaturing.

[0033] According to an embodiment of the present application, an anti - pollution material 170 can be covered above the indicating holes 121 - 128. In some embodiments, the anti - pollution material is cotton fiber; in other embodiments, the anti - pollution material is filter paper fiber. The anti - pollution material can not only isolate the indicating material 130 from contact with water molecules in the air, preventing the indicating material from absorbing moisture in the air and denaturing during storage, but also ensure that water molecules can quickly penetrate into the indicating holes 121 - 128 during the experiment to contact the indicating material 130.

[0034] According to an embodiment of the present application, the indicating strip 100 further includes an adhesive 140 for adhering the indicating strip to the carrying device. The adhesive 140 is attached to the side of the bottom strip 110 without the indicating holes 121 - 128. In some embodiments, the adhesive 140 is made of a non - trace sticky material, enabling the indicating strip 100 to be firmly adhered to the surface of the carrying device and allowing the indicating strip 100 to be torn off from the carrying device at any time without leaving a trace. In some embodiments, the surface of the adhesive 12 can be covered with a material such as a smooth plastic paper that can be completely separated from the adhesive and has good flexibility to facilitate rolling up and storing the indicating strip including the adhesive.

[0035] Figure 2 is a reagent adding indicating strip roll according to an embodiment of the present invention. As Figure 2 shown, in some embodiments, the indicating strip 100 can be rolled into an indicating strip roll for convenient storage. When in use, only the required length of the indicating strip on the indicating strip roll needs to be intercepted.

[0036] According to an embodiment of the present application, the indicating strip can be adhered to a warming plate, where the warming plate can be a disposable experimental consumable or a reusable experimental consumable. The warming plate includes a plate body 120 and a plurality of liquid adding tanks 221 - 2215. The plurality of indicating holes 121 - 1215 on the indicating strip respectively correspond one - to - one with the plurality of liquid adding tanks 221 - 2215 on the warming plate, and each indicating hole indicates whether a liquid is added to its corresponding liquid adding tank.

[0037] In some embodiments, the indicating strip and the warming plate are integrated, and the warming plate with the indicating strip is a disposable experimental consumable. As Figure 3As shown, the incubation plate with an indicator strip includes a plurality of indicator holes 121 - 1215, a plate body 210, and a plurality of sample addition slots 221 - 2215. The plurality of indicator holes 121 - 1215 respectively correspond one - to - one with the plurality of liquid addition slots 221 - 2215, and each indicator hole indicates whether liquid is added to its corresponding liquid addition slot.

[0038] According to an embodiment of the present application, the method of using the sample addition indicator strip includes:

[0039] 1. Cut a part of the sample addition indicator strip as described above;

[0040] 2. Paste the cut sample addition indicator strip on the incubation plate so that the positions of the plurality of indicator holes of the sample addition indicator strip correspond to the plurality of liquid addition slots of the incubation plate (as Figure 3 shown).

[0041] According to an embodiment of the present application, the method of adding liquid using the sample addition indicator strip described above includes: before or after adding liquid to the liquid addition slot, contact the indicator hole of the sample addition indicator strip corresponding to the liquid addition slot with the liquid addition head for adding liquid.

[0042] According to an embodiment of the present application, the method of adding liquid using the incubation plate described above includes: before or after adding liquid to the liquid addition slot, contact the indicator hole of the sample addition indicator strip corresponding to the liquid addition slot with the liquid addition head for adding liquid.

[0043] As Figure 3 shown, in some embodiments, when using the indicator hole to indicate the addition situation of the liquid reagent in its corresponding liquid addition slot, the following steps can be referred to:

[0044] 1. Cut an appropriate length of the indicator strip. Among them, the number of indicator holes in the cut indicator strip should be the same as the number of liquid addition slots on the device to be indicated.

[0045] 2. Paste the indicator strip on the incubation plate. Before pasting firmly, adjust the position of the indicator strip so that each indicator hole corresponds to each sample addition slot.

[0046] 3. Add the reagent to the liquid addition slot and blow - suck and mix the reagent according to the experimental requirements.

[0047] 4. Without changing the liquid addition head of the pipette, directly contact the top of the liquid addition head of the pipette adding the reagent with its corresponding indicator hole.

[0048] 5. The property of the indicator hole changes, for example, the color of the indicator hole changes from blue to red, indicating that the sample addition action for this liquid addition slot has been completed.

[0049] Among them, in some embodiments, steps 3 and 4 can be interchanged, first use the liquid addition head of the pipette with the reagent to touch the indicator hole, and then add the reagent therein to the corresponding liquid addition slot.

[0050] According to an embodiment of the present application, as Figure 2 shown, every time a liquid reagent is added to a liquid addition tank, immediately use the liquid addition pipette tip to touch the corresponding indication hole. For example, reagents have been respectively added to the liquid addition tanks 221-224 and the indication holes 121-124, and the indication holes 121-124 have changed. After adding a reagent to the liquid addition tank 225, immediately use the pipette tip of the pipette 300 for adding the reagent to touch the corresponding indication hole 125. When the indication hole 125 changes from blue to pink, it indicates that the liquid addition to the liquid addition tank 225 is completed, and the liquid addition tanks 226-2215 are to be added with liquid.

[0051] This method can ensure that the sample reagent is added to each liquid addition slot and there will be no repeated addition. Even if there is a short pause, the continuous liquid addition position can be easily identified without the support of additional consumables, saving costs.

[0052] The above embodiments are only for illustrating the present invention and are not intended to limit the present invention. Those of ordinary skill in the relevant technical field can still make various changes and modifications without departing from the scope of the present invention. Therefore, all equivalent technical solutions should also fall within the scope of the disclosure of the present invention.

Claims

1. A method for using a sample addition indicator strip, the sample addition indicator strip comprising: A bottom strip configured to be placed on a carrier device having a plurality of liquid addition grooves; And A plurality of indicator holes provided on the bottom strip, wherein the positions of the plurality of indicator holes correspond to the plurality of liquid addition grooves of the carrier device; wherein, among the plurality of indicator holes, there is an indicator material suitable for indicating whether it contacts the liquid added to the liquid addition groove, above the plurality of indicator holes there is an anti-pollution material which is filter paper fiber, and the side of the bottom strip without indicator holes has an adhesive; the bottom strip has good flexibility and can be placed in a roll; the indicator holes have a concave structure; the indicator holes are arranged along the long side direction of the bottom strip, and the centers of the plurality of indicator holes are arranged in a straight line; the method includes: Cutting a part of the sample addition indicator strip; and Pasting the cut part of the sample addition indicator strip on a incubation plate such that the positions of the plurality of indicator holes of the sample addition indicator strip correspond to the plurality of liquid addition grooves of the incubation plate; Before or after adding liquid to the liquid addition groove, contacting the liquid addition head for adding liquid with the indicator hole of the sample addition indicator strip corresponding to the liquid addition groove.

2. The method according to claim 1, wherein the sample addition indicator strip further comprises a waterproof layer covering the bottom strip.

3. The method according to claim 1, wherein the indicator material is anhydrous cobalt chloride.

4. An incubation plate, comprising: A plate body; A plurality of liquid addition grooves provided on the plate body; And A sample addition indicator strip in the method for using a sample addition indicator strip according to any one of claims 1-3; Wherein, the positions of the plurality of indicator holes of the sample addition indicator strip in the method for using a sample addition indicator strip according to any one of claims 1-3 correspond to the plurality of liquid addition grooves.

5. A method for adding liquid using the incubation tray described in claim 4, comprising: Before or after adding liquid to the liquid addition groove, contacting the liquid addition head for adding liquid with the indicator hole of the sample addition indicator strip in the method for using a sample addition indicator strip according to any one of claims 1-3 corresponding to the liquid addition groove.

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