A miniature fluorescence microscopy module

Abstract

The present invention discloses a miniature fluorescence microscopy imaging module, comprising an imaging lens module and an illumination device; the imaging lens module comprises an objective lens and a tube lens symmetrically arranged from the object side to the image side, an image sensor with a high spatial sampling rate arranged on the image side of the tube lens, and an emission filter arranged in front of the objective lens, or between the objective lens and the tube lens, or between the tube lens and the image sensor; the illumination device is separately arranged from the imaging lens module to form uniform illumination of the sample; the excitation light path of the illumination device is separately arranged from the detection light path of the imaging lens module. The present invention adopts an approximately symmetrical structure to design the objective lens and the tube lens, combined with a sensor with a high spatial sampling rate, to achieve high-resolution imaging at low magnification. Such a design can effectively shorten the conjugate distance, solving the problem that the image distance is still very long after the microscope is miniaturized. The three excitation illumination schemes eliminate the need to add excitation filters and dichroic mirrors, and can meet the illumination requirements of different observation samples.

Description

Technical Field

[0001] The present invention relates to the field of optical imaging, and in particular to a miniature fluorescence microscopy imaging module. Background Art

[0002] Fluorescence microscopes are often used in the life sciences. These microscopes typically consist of a light source, excitation filter, dichroic mirror, objective lens, emission filter, tube lens, and detector. Excitation light from the light source passes through the excitation filter, which is then reflected by the dichroic mirror and directed to the objective lens, where it is focused onto the sample surface. The resulting fluorescence signal is collected by the objective lens, passes through the dichroic mirror and filter, and then imaged onto the detector surface by the tube lens. These microscopes typically have a certain magnification, which inevitably results in a short object distance and a long image distance. Sufficient space must be left in the image space to accommodate these optical path requirements. Furthermore, the light source requires a mains power connection, and detectors such as CCDs or CMOS also require either mains power or a computer power supply. Image display and storage also require a computer connection. Furthermore, microscopes must have a focusing mechanism, either manual or automatic, and the mechanical structure is complex, taking up considerable space. Because of these factors, fluorescence microscopes are typically large, complex, and expensive, and lack portability.

[0003] The core components of fluorescence microscopes for imaging are the objective lens and the tube lens. The main factor limiting miniaturization is the system's high magnification, which typically results in a long focal length for the tube lens. Furthermore, the filter set continues to be miniaturized using the same setup as before, without further consideration for miniaturization. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a miniature fluorescence microscopy imaging module in view of the defects of the prior art.

[0005] The technical solution adopted by the present invention to solve the technical problem is: constructing a miniature fluorescence microscopy imaging module, including an imaging lens module and an illumination device;

[0006] The imaging lens module includes an objective lens and a cylindrical lens symmetrically arranged from the object side to the image side, an image sensor with a high spatial sampling rate arranged on the image side of the cylindrical lens, and an emission filter arranged in front of the objective lens, or between the objective lens and the cylindrical lens, or between the cylindrical lens and the image sensor;

[0007] The lighting device is separately provided from the imaging lens module to form uniform illumination for the sample;

[0008] The excitation light path of the illumination device is separately arranged from the detection light path of the imaging lens module; the detection light path is, from the object side to the image side, a sample, an emission filter, an objective lens, a tube lens, and an image sensor; or a sample, an objective lens, a tube lens, an emission filter, and an image sensor; or a sample, an objective lens, a tube lens, an emission filter, and an image sensor.

[0009] Preferably, in the micro fluorescence microscopy imaging module of the present invention, the objective lens and the tube lens constitute a lens group, the distance between the object plane and the image plane of the lens group on the optical axis is TTL, and the distance between the object side surface and the image side surface of the lens group on the optical axis is TD, which satisfies the following relationship: 0.15≤TD / TTL≤0.9;

[0010] The focal length f1 of the objective lens and the focal length f2 of the tube lens satisfy the following relationship: 0.1≤f1 / f2≤10;

[0011] The optical tube length of the lens assembly satisfies the following relationship: 0.2≤TTL / (f1+f2)≤15;

[0012] The distance L from the object principal plane of the objective lens to the object plane Obj Satisfy the following relationship: 0.5f1≤L Obj ≤1.5f1;

[0013] The distance L between the image side principal plane of the tube lens and the image plane Ima Satisfies the following relationship: 0.5f2≤L Ima ≤1.5f2.

[0014] Preferably, in the micro fluorescence microscopy module of the present invention, the lighting device includes an excitation light source arranged at the periphery of the objective lens, and a light-uniform light guide plate corresponding to the excitation light source;

[0015] The light emitted by the excitation light source forms uniform illumination for the sample after passing through the uniform light guide plate.

[0016] Preferably, in the micro fluorescence microscopy imaging module described in the present invention, the excitation light sources include multiple ones, which are evenly distributed in a ring shape around the periphery of the objective lens; the uniform light guide plate is an annular light guide plate arranged in the light emitting direction of the excitation light source; and the end face of the annular light guide plate is basically flush with the end face of the objective lens close to the sample.

[0017] Preferably, in the micro fluorescence microscopy module of the present invention, the lighting device includes an excitation light source arranged on one side of the sample; the light emitted by the excitation light source irradiates the target area from the side of the sample.

[0018] Preferably, in the miniature fluorescence microscopy imaging module of the present invention, the miniature fluorescence microscopy imaging module further comprises a support arm provided on one side of the imaging lens module; and the excitation light source is mounted on the support arm.

[0019] Preferably, in the miniature fluorescence microscopy imaging module described in the present invention, the lighting device is arranged below the sample, including a first excitation light source corresponding to the periphery of the objective lens field of view, a first uniform light guide plate corresponding to the first excitation light source, and a baffle plate arranged corresponding to the edge of the objective lens field of view, and the light is irradiated from the bottom side of the sample to the target area.

[0020] Preferably, in the micro fluorescence microscopy module of the present invention, the lighting device further comprises a second excitation light source correspondingly arranged under the field of view of the objective lens, and a second light-homogenizing light guide plate corresponding to the second excitation light source, and the light is irradiated from directly below the sample to the target area;

[0021] The shielding plate isolates the first excitation light source, the second excitation light source, the first light-uniform light guide plate, and the second light-uniform light guide plate respectively.

[0022] Preferably, in the miniature fluorescence microscopy module of the present invention, the miniature fluorescence microscopy module further comprises a support base, which comprises a support arm and a base fixed to the end of the support arm; the support arm fixes the sample from the side.

[0023] Preferably, in the micro fluorescence microscopy imaging module of the present invention, the excitation light source is a laser diode or a light emitting diode;

[0024] The objective lens and the tube lens are respectively composed of at least three lenses.

[0025] Preferably, in the miniature fluorescence microscopy imaging module of the present invention, the miniature fluorescence microscopy imaging module further comprises a driver circuit board with a USB interface for driving the lighting device and the image sensor; and or

[0026] A protective glass is provided in front of the objective lens.

[0027] Preferably, in the miniature fluorescence microscopy module of the present invention, the miniature fluorescence microscopy module further comprises a focusing motor, which moves the objective lens, or the tube lens, or the entire objective lens, emission filter and tube lens to achieve focusing.

[0028] By implementing the present invention, the following beneficial effects are achieved:

[0029] The present invention is based on the structure of a traditional infinity-corrected fluorescence microscope, adopts an approximately symmetrical structure to design the objective lens and the tube lens, and combines it with a sensor with a high spatial sampling rate to achieve high-resolution imaging at low magnification. Such a design can effectively shorten the conjugate distance, solving the problem that the image distance is still very long after the microscope is miniaturized. The three excitation illumination schemes eliminate the need to add excitation filters and dichroic mirrors, and can meet the lighting requirements of different observation samples. The three different positions of the emission filter reduce aberrations and ensure the quality of imaging. In addition, for the sake of portability and simple operation, the driving circuit of the entire microscope only requires a simple USB connection for power supply, and the image of the image sensor can also be connected to a computer or mobile phone through the USB for display and storage. In addition, the microscopic imaging module can also be added with a focusing motor. Since the objective lens and the tube lens are small in size and light in weight, a mature focusing motor such as a voice coil motor can be used to drive the lens group to focus. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The present invention will be further described below with reference to the accompanying drawings and embodiments, in which:

[0031] Figure 1 1 is a schematic structural diagram of a first embodiment of a miniature fluorescence microscopy imaging module of the present invention;

[0032] Figure 2 2 is a schematic structural diagram of a second embodiment of a miniature fluorescence microscopy imaging module according to the present invention;

[0033] Figure 3 2 is a schematic structural diagram of a third embodiment of a miniature fluorescence microscopy imaging module according to the present invention;

[0034] Figure 4 2 is a schematic structural diagram of a fourth embodiment of a miniature fluorescence microscopy imaging module according to the present invention. DETAILED DESCRIPTION

[0035] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, specific embodiments of the present invention are now described in detail with reference to the accompanying drawings.

[0036] It should be understood that the directions or positional relationships indicated by “front”, “rear”, “up”, “down”, etc. are based on the directions or positional relationships shown in the accompanying drawings, and are constructed and operated in a specific direction. They are only for the convenience of describing the present technical solution, and do not indicate that the device or element referred to must have a specific direction. Therefore, they cannot be understood as a limitation on the present invention.

[0037] like Figure 1-4 As shown, the present invention constructs a micro fluorescence microscopy imaging module, including an imaging lens module and an illumination device;

[0038] The imaging lens module includes an objective lens 18 and a tubular lens 16, symmetrically arranged from the object side to the image side; an image sensor 28 with a high spatial sampling rate, located on the image side of the tubular lens 16; and an emission filter 17, located in front of the objective lens 18, or between the objective lens 18 and the tubular lens 16, or between the tubular lens 16 and the image sensor 28. The illumination device is separate from the imaging lens module to provide uniform illumination of the sample 10. The excitation light path of the illumination device is separate from the detection light path of the imaging lens module; the detection light path from the object side to the image side is sample 10, emission filter 17, objective lens 18, tubular lens 16, and image sensor 28; or sample 10, objective lens 18, emission filter 17, tubular lens 16, and image sensor 28; or sample 10, objective lens 18, tubular lens 16, emission filter 17, and image sensor 28.

[0039] In some embodiments, the objective lens 18 and the tube lens 16 are each composed of at least three lenses, which can be made of glass or plastic. The lens surface can be spherical or aspherical depending on the aberration correction requirements. The objective lens 18 and the tube lens 16 form a lens assembly. This lens assembly is an infinity-corrected microscope with a nearly symmetrical design to optimize aberrations and achieve a resolution of 1 μm at low magnification. Low magnification effectively reduces the image distance, enabling miniaturization of the microscope and increasing the field of view.

[0040] The distance between the object plane and the image plane of the lens assembly on the optical axis is TTL, and the distance between the object side surface and the image side surface of the lens assembly on the optical axis is TD, which satisfy the following relationship: 0.15≤TD / TTL≤0.9;

[0041] The focal length f1 of the objective lens 18 and the focal length f2 of the tube lens 16 satisfy the following relationship: 0.1≤f1 / f2≤10;

[0042] The optical tube length of the lens group satisfies the following relationship: 0.2≤TTL / (f1+f2)≤15;

[0043] The distance L from the object principal plane of the objective lens 18 to the object plane Obj Satisfy the following relationship: 0.5f1≤L Obj ≤ 1.5f1;

[0044] The distance L from the image side principal plane of the tube lens 16 to the image plane Ima Satisfies the following relationship: 0.5f2≤L Ima ≤ 1.5f2.

[0045] In some embodiments, the emission filter 17 can be an absorption filter or an interference filter. When it is arranged in front of the objective lens 18, it can shorten the distance between the objective lens 18 and the tube lens 16, which helps to connect the pupils and better correct aberrations; when it is arranged between the objective lens 18 and the tube lens 16, since the light incident on the filter is parallel light, aberrations can be avoided; when it is arranged between the tube lens 16 and the image sensor 28, when working in the afocal mode, the incident angle of the light is small, which is conducive to better realizing the function of filtering out light.

[0046] In some embodiments, the micro fluorescence microscopy module further includes a driver circuit board 11 with a USB interface for driving the lighting device, image sensor 28, and focus motor 27. The USB interface can be connected to a computer, mobile phone, or other device to provide power and display images.

[0047] In some embodiments, the lighting device includes an excitation light source 19 and a light-homogenizing light guide plate 29 corresponding to the excitation light source 19. The excitation light source 19 can be a laser diode or a light-emitting diode. The light-homogenizing light guide plate 29 can be made of materials such as PC (polycarbonate), PMMA (acrylic), and glass. The light homogenization effect is achieved by treating the surface of the material into a frosted surface.

[0048] In some embodiments, the illumination device has the following illumination modes: 1) dark field illumination with annular light reflected above the sample, where the annular light is distributed around the periphery of the objective lens and is flush with the lower surface of the objective lens;

[0049] 2) The sample is illuminated from the side, with the light from the light source at a 0° angle being incident on the center of the sample field of view within a 10° angle relative to the horizontal plane. Preferably, the light from the light source at a 0° angle is incident on the center of the sample field of view along the horizontal direction;

[0050] 3) Annular transmissive darkfield illumination below the sample: a ring of light is distributed below the sample and around the periphery of the field of view. These three illumination methods achieve uniform illumination of the sample surface while preventing the excitation light from directly entering the lens and generating stray light, which can affect imaging.

[0051] In addition, the three illumination modes eliminate the need for dichroic mirrors and excitation filters, which can effectively reduce space and increase the compactness of the structure.

[0052] In some embodiments, a protective glass 15 is provided in front of the objective lens 18 to effectively protect the entire imaging module from external pollution such as dust and water vapor.

[0053] By implementing the present invention, the following beneficial effects are achieved:

[0054] 1) No additional excitation filters or dichroic mirrors are required. Fluorescent materials typically have a single spectral absorption spectrum, while laser diodes and light-emitting diodes have very narrow spectra. Therefore, a light source with a wavelength appropriate to the fluorescent material can be selected, eliminating the need for additional excitation filters. Furthermore, because the excitation and detection light paths are separate, a dichroic mirror is not required to separate the excitation light from the fluorescence. This setup effectively reduces the overall size of the fluorescence microscope and makes the setup simpler.

[0055] 2) The structure is miniature, compact, and simple, making it easy to assemble and adjust. The microscope's nearly symmetrical structure, combined with a high-spatial sampling rate image sensor, enables high-resolution imaging with a large field of view at low magnification. This solves the problem of long image distance, facilitates shortening the conjugate distance, and enables miniaturization of the imaging module. Furthermore, the aforementioned elimination of the need for excitation filters and dichroic mirrors simplifies the structure while further reducing the space occupied, resulting in a more compact design.

[0056] 3) Easy to operate and portable. The fluorescence microscopy module operates simply via USB power, eliminating the need for mains power. Furthermore, it can be directly connected to a mobile phone to display and store images, making it easy to carry outdoors.

[0057] In some embodiments, the micro-fluorescence microscopy module may further include a focus motor 27. Optionally, the focus motor 27 may be a voice coil motor, a stepper motor, an ultrasonic motor, a memory alloy motor, or the like. The focus motor 27 can move the objective lens 18 to achieve focus without affecting the system magnification. If the effect on the system magnification is not a concern, the focus motor 27 can also drive the tube lens 16, or the entire assembly consisting of the objective lens 18, emission filter 17, and tube lens 16, to achieve focus.

[0058] When the focusing motor 27 drives the objective lens 18 or the tube lens 16 to achieve focusing, the position of the object plane of the micro-fluorescence microscopy imaging module is changed. Among them, the object plane closest to the micro-fluorescence microscopy imaging module is the near-focus object plane, and the object plane farthest from the micro-fluorescence microscopy imaging module is the far-focus object plane, and the direction away from the imaging lens module is the positive direction. The objective lens 18 and the tube lens 16 form a lens group, and the object side of the lens group includes a limiting surface. When there is a protective glass 15 on the object side of the lens group, the object surface of the protective glass 15 is the limiting surface; when there is no protective glass 15 on the object side of the lens group, the end face of the imaging lens module housing or other mechanical structures that cooperate with the imaging lens module is the limiting surface. The near-focus object plane is within the range of ± 50μm of the limiting surface, and the distance between the far-focus object plane and the limiting surface is ≥220μm.

[0059] The stroke of the focus motor 27 is ≥300 μm and ≤600 μm. When the focus motor 27 moves the objective lens 18, the focusing distance is equal to the stroke of the focus motor 27. Within the focusing range, the minimum distance between the objective lens 18 and the tube lens 16 is ≥50 μm. When the protective glass 15 is present, the minimum distance between the objective lens 18 and the protective glass 15 is ≥50 μm within the focusing range.

[0060] Adopting the above parameters has the following beneficial effects:

[0061] 1) The near-focus object plane can cover the close-range area of ​​the protective glass 15, allowing for imaging of close objects. At the same time, the far-focus object plane can exceed the cover glass commonly used in microscopes, meeting the needs of biomedical imaging.

[0062] 2) The travel of the focus motor 27 can effectively cover the dimensional tolerances of the components within the module due to processing and installation, improving the manufacturability of mass production;

[0063] 3) Minimum clearances are maintained between the objective lens 18 and the protective glass 15 and the tube lens 16, thereby improving the reliability of the imaging lens module and preventing damage to the internal components of the module due to collision when the focus motor 27 exceeds the rated stroke;

[0064] 4) While achieving the above beneficial effects, the module is miniaturized.

[0065] In addition, when the focusing motor 27 moves the objective lens 18, the position of the tube lens 16 is fixed. At this time, the near-focus object plane is the object plane focused on by the lens group when the distance between the objective lens 18 and the tube lens 16 is the smallest;

[0066] When the focusing motor 27 drives the tube lens 16 to move, the position of the objective lens 18 is fixed. At this time, the near-focus object plane is the object plane focused by the lens group when the distance between the objective lens 18 and the tube lens 16 is the smallest.

[0067] The specific embodiments of the present invention are further described in detail below in conjunction with the drawings and examples. The following examples are used to illustrate the present invention but are not used to limit the scope of the present invention.

[0068] In the first embodiment, as Figure 1As shown, the detection optical path from the object side to the image side is, in order, the sample 10, protective glass 15, objective lens 18, tube lens 16, emission filter 17, and image sensor 28. The objective lens 18 is fixed in the voice coil motor 27, and the tube lens 16 is fixed in the tube lens fixture 12. The focal length f1 of the objective lens 18 is 2.6 mm, and the focal length f2 of the tube lens 16 is 5.1 mm. The distance TTL from the object plane to the image plane of the lens assembly on the optical axis is 10.2 mm, and the distance TD from the object side surface to the image side surface on the optical axis is 7.2 mm. The emission filter 17 is fixed in the housing 26, and the image sensor 28 is directly integrated on the driver circuit board 11.

[0069] The lighting device includes an excitation light source 19 arranged on the periphery of the objective lens 18, and a uniform light guide plate 29 corresponding to the excitation light source 19. The light emitted by the excitation light source 19 forms uniform illumination of the sample 10 after passing through the uniform light guide plate 29. In this embodiment, the excitation light source 19 includes a plurality of excitation light sources 19, which are evenly distributed in a ring shape on the periphery of the objective lens 18. The uniform light guide plate 29 is an annular light guide plate arranged in the light emitting direction of the excitation light source 19, and the end face of the annular light guide plate is basically flush with the end face of the objective lens 18 close to the sample 10, forming an annular light reflection dark field illumination above the sample. Preferably, the excitation light source 19 is an LED lamp, and the LED lamp and the uniform light guide plate 29 are fixed on the housing 26. The LED lamp is at least two LED light sources distributed at equal angles along the circumferential direction. The specific number of light sources can be set according to parameters such as the illumination uniformity and illumination value of the sample surface.

[0070] The driver circuit board 11 has a USB port that can be connected directly to a computer USB port or to a mobile phone via an OTG cable. When the circuit board is powered on, the fluorescence microscope is in operation. The LED lamp 19 emits excitation light, which passes through the uniform light guide plate 29 and the protective glass 15 and then illuminates the sample 10, forming a uniform illumination. The fluorescent substance is excited to produce a fluorescent signal, which is collected by the objective lens 18 after passing through the protective glass 15. It is then focused by the tube lens 16 and filtered by the emission filter 17 before being imaged on the image sensor 28. The signal from the image sensor 28 is processed by the driver circuit board 11 and displayed on a computer or mobile phone via a USB data cable, allowing the operator to view the fluorescent image in real time and save it as needed.

[0071] The voice coil motor 27 is located around the objective lens 18 and is fixed to the housing 26. The driver circuit board 11 drives the voice coil motor 27. The energized coil inside the voice coil motor 27 generates an Ampere force under the action of the magnetic field, which enables the objective lens 18 to move axially, realizing the automatic focusing function.

[0072] In the second embodiment, as Figure 2As shown, compared with the first embodiment, the positions of the illumination device, emission filter 17, and focus adjustment target are different. The illumination device includes an excitation light source 19 positioned to one side of the sample 10. Light emitted by the excitation light source 19 illuminates the target area from the side of the sample 10. The micro-fluorescence microscopy module also includes a support arm 24 positioned to one side of the imaging lens module, on which the excitation light source 19 is mounted.

[0073] In this embodiment, the micro fluorescence microscopy module also includes a support base, which includes a support arm 24 and a base 23 fixed to the end of the support arm 24. The excitation light source 19 is a light-emitting diode fixed to the support arm 24. The light it emits illuminates the target area from the side of the sample. The structure is simple and can achieve a uniform lighting effect. The number of excitation light sources 19 can be distributed around the sample according to actual lighting needs. The support arm 24 is connected to the driver circuit board 11 to provide circuit support for the excitation light source 19. The base 23 provides support for the entire fluorescence microscopy module. The emission filter 17 is arranged between the objective lens 18 and the tube lens 16 and is fixed to the housing 26. The emission filter 17 will filter out other unnecessary wavelengths of light passing through the objective lens 18 to avoid aberrations. The voice coil motor 27 is fixed to the housing 26 around the tube lens 16, driving the tube lens 16 to move axially to achieve automatic focusing.

[0074] In the third embodiment, Figure 3 As shown, compared with the first embodiment, the positions of the illumination device and emission filter 17 and the setting of the focus target are different. The illumination device is arranged below the sample 10 and includes at least two first excitation light sources 19 arranged corresponding to the periphery of the field of view of the objective lens 18, a first light-uniforming light guide plate 29 corresponding to the first excitation light sources 19, and a shielding plate 30 arranged corresponding to the edge of the field of view of the objective lens 18. Light is irradiated from the lower side of the sample 10 to the target area.

[0075] The illumination device also includes at least one second excitation light source 19A disposed under the field of view of the objective lens 18, and a second uniform light guide plate 29A corresponding to the second excitation light source 19A. Light is irradiated from directly below the sample 10 to the target area. A shielding plate 30 isolates the first and second excitation light sources 19, 19A, and the first and second uniform light guide plates 29, 29A, respectively.

[0076] In this embodiment, the excitation light sources include multiple ones, which are evenly distributed in a ring shape outside the field of view of the objective lens 18, and the uniform light guide plate is an annular light guide plate arranged in the light emitting direction of the excitation light source.

[0077] In this embodiment, the micro fluorescence microscopy module further includes a support base, which includes a support arm 24 and a base 23 fixed to the end of the support arm 24. The support arm 24 fixes the sample 10 from the side.

[0078] In this embodiment, both the first excitation light source 19 and the second excitation light source 19A are LEDs, fixed to the base 23 and positioned outside the objective lens' field of view. The light they emit passes through the first uniform light guide plate 29 and illuminates the target area from below the sample, creating transmitted darkfield illumination. This illumination method is suitable for uniform illumination of transparent samples and also prevents direct illumination light from entering the objective lens. The shielding plate 30 prevents light from the LED 19 from directly incident on the sample surface from the inside, while also isolating the first uniform light guide plate 29 from the second uniform light guide plate 29A. Furthermore, an LED 19A is positioned directly below the sample, independently controllable from the LED 19. When the LED 29A is turned on, light from below the sample passes through the second uniform light guide plate 29A and directly illuminates the sample, achieving fluorescence excitation. These two illumination methods can be selected for different samples or specific needs. The support arm 24 is connected to the driver circuit board 11, providing circuit support for the ring-shaped light sources, namely the first excitation light source 19 and the second excitation light source 19A. The emission filter 17 is positioned in front of the objective lens 18, shortening the distance between the objective lens 18 and the tube lens 16. This facilitates pupil alignment and improves aberration correction. The voice coil motor 27 is positioned differently. Located around the periphery of the mounting brackets for the objective lens 18, tube lens 16, and emission filter 17, it drives the entire lens assembly, enabling automatic focusing.

[0079] In the fourth embodiment, Figure 4 Compared to the second embodiment, the voice coil motor 27 is eliminated, and the objective lens 18 is fixed to the objective lens holder 13, making the entire microscope structure simpler and more miniaturized. The protective glass 15 is omitted, reducing stray light from entering the lens. The light source 22 is a laser diode with a small divergence angle, effectively concentrating the light incident on the sample surface.

[0080] The present invention is described by way of specific embodiments. It should be understood by those skilled in the art that various modifications and equivalent substitutions may be made to the present invention without departing from the scope of the present invention. In addition, various modifications may be made to the present invention for specific circumstances or specific conditions without departing from the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed, but is intended to include all embodiments falling within the scope of the claims of the present invention.

Claims

1. A miniature fluorescence microscopy imaging module, characterized in that: Includes an imaging lens module and an illumination device, excluding excitation filters and dichroic mirrors; The imaging lens module comprises an objective lens (18) and a tube lens (16) symmetrically arranged in sequence from the object side to the image side, an image sensor (28) with a high spatial sampling rate arranged on the image side of the tube lens (16), and an emission filter (17) arranged in front of the objective lens (18), or between the objective lens (18) and the tube lens (16), or between the tube lens (16) and the image sensor (28); The lighting device is arranged separately from the imaging lens module to form uniform illumination for the sample (10); The lighting device is arranged above the sample (10), and includes a plurality of excitation light sources (19) arranged on the periphery of the objective lens (18) and evenly distributed in a ring shape, and a uniform light guide plate (29) corresponding to the excitation light source (19); light emitted by the excitation light source (19) passes through the uniform light guide plate (29) to form uniform illumination for the sample (10); Alternatively, the lighting device includes an excitation light source (19) disposed on one side of the sample (10); light emitted by the excitation light source (19) is irradiated from the side of the sample (10) to the target area; Alternatively, the lighting device is arranged below the sample (10), and includes a plurality of first excitation light sources (19) correspondingly arranged below the periphery of the field of view of the objective lens (18) and uniformly distributed in a ring shape, a first uniform light guide plate (29) corresponding to the first excitation light source (19), and a shielding plate (30) arranged corresponding to the edge of the field of view of the objective lens (18), and light is irradiated from the lower side of the sample (10) to the target area; The excitation light path of the illumination device is separately arranged from the detection light path of the imaging lens module; the detection light path is, from the object side to the image side, a sample (10), an emission filter (17), an objective lens (18), a tube lens (16), and an image sensor (28); or a sample (10), an objective lens (18), an emission filter (17), a tube lens (16), and an image sensor (28); or a sample (10), an objective lens (18), a tube lens (16), an emission filter (17), and an image sensor (28).

2. The micro fluorescence microscopy module according to claim 1, characterized in that: The objective lens (18) and the tube lens (16) form a lens group, the distance from the object plane to the image plane of the lens group on the optical axis is TTL, and the distance from the object side surface to the image side surface of the lens group on the optical axis is TD, which satisfies the following relationship: 0.15≤TD / TTL≤0.9; The focal length f1 of the objective lens (18) and the focal length f2 of the tube lens (16) satisfy the following relationship: 0.1≤f1 / f2≤10; The optical tube length of the lens assembly satisfies the following relationship: 0.2≤TTL / (f1+f2)≤15; The distance L from the object principal plane of the objective lens (18) to the object plane Obj Satisfy the following relationship: 0.5f1≤L Obj ≤1.5f1; The distance L between the image side principal plane of the tube lens (16) and the image plane Ima Satisfies the following relationship: 0.5f2≤L Ima ≤1.5f2.

3. The micro fluorescence microscopy module according to claim 1, characterized in that: When the lighting device is arranged above the sample (10), the uniform light guide plate (29) is an annular light guide plate arranged in the light emitting direction of the excitation light source (19); and the end face of the annular light guide plate is substantially flush with the end face of the objective lens (18) close to the sample (10).

4. The micro fluorescence microscopy module according to claim 1, characterized in that: When the lighting device includes an excitation light source (19) arranged on one side of the sample (10), the micro fluorescence microscopy imaging module further includes a support arm (24) arranged on one side of the imaging lens module; the excitation light source (19) arranged on one side of the sample (10) is mounted on the support arm (24).

5. The micro fluorescence microscopy module according to claim 1, characterized in that: When the lighting device is arranged below the sample (10), the lighting device arranged below the sample (10) further includes a second excitation light source (19A) correspondingly arranged under the field of view of the objective lens (18), and a second uniform light guide plate (29A) corresponding to the second excitation light source (19A), and light is irradiated from directly below the sample (10) to the target area; The shielding plate (30) isolates the first excitation light source (19) and the second excitation light source (19A), the first uniform light guide plate (29) and the second uniform light guide plate (29A) respectively.

6. The micro fluorescence microscopy module according to claim 5, characterized in that: The micro fluorescence microscopy module further comprises a support base, which comprises a support arm (24) and a base (23) fixed to the end of the support arm (24); the support arm (24) fixes the sample (10) from the side thereof.

7. The micro fluorescence microscopy module according to any one of claims 3 to 6, characterized in that: The excitation light source (19) is a laser diode or a light emitting diode; The objective lens (18) and the tube lens (16) are respectively composed of at least three lenses.

8. The miniature fluorescence microscopy imaging module according to any one of claims 1 to 6, characterized in that: The micro fluorescence microscopy module further includes a driver circuit board (11) with a USB interface for driving the lighting device and the image sensor (28); and or A protective glass (15) is provided in front of the objective lens (18).

9. The micro fluorescence microscopy imaging module according to claim 1, characterized in that: The micro fluorescence microscopy module further includes a focusing motor (27) that moves the objective lens (18), or the tube lens (16), or the entire objective lens (18), the emission filter (17) and the tube lens (16) to achieve focusing.

Images