Droplet detection system, droplet control device and droplet detection method

Abstract

The present invention relates to a droplet detection system, a droplet control device and a droplet detection method. The droplet control device comprises: a mounting body, an infusion component, a conflict component and a detection chip, the mounting body is provided with a mounting cavity for placing the detection chip, the mounting body is provided with a liquid inlet hole, and the liquid inlet holes are connected with the mounting cavity, the conflict component is sleeved on the outside of the infusion component, the infusion component is introduced into the mounting cavity through the liquid inlet hole, the conflict component conflicts with the hole wall of the liquid inlet hole, the infusion component is used to transport sample droplets, and the detection chip is used to detect the sample droplets transported by the infusion component. The above-mentioned droplet control device effectively avoids leakage of the droplet control device and improves the detection effect of the droplet control device on the sample.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to a droplet detection system, a droplet control device and a droplet detection method. Background Art

[0002] With the development of biomedical technology, many biomedical applications rely on high-throughput determination of samples combined with reagents. This high-throughput detection demand can be met by droplet detection technology. At present, the traditional droplet detection technology is to clamp the detection chip by a clamp, the infusion tube is connected to the clamp, and the sample to be tested is sent to the detection chip in the form of droplets through the infusion tube for detection. However, when the infusion tube is connected to the clamp, a rubber pad is added between the clamp and the infusion tube, that is, the infusion tube passes through the rubber pad and extends into the inside of the clamp. At this time, the rubber pad will generate elastic force under the interaction between the infusion tube and the clamp, which causes the infusion tube to be easily displaced under the action of the elastic force (for example: the infusion tube directly pops out of the clamp under the action of the elastic force), so that the sample leaks during the process of being introduced into the clamp with the infusion tube, reducing the detection effect of the sample. Summary of the invention

[0003] Based on this, it is necessary to provide a droplet detection system, a droplet control device and a droplet detection method to address the problem of reduced detection effect on samples.

[0004] A droplet control device, the droplet control device comprising: a mounting body, an infusion component, a resistance component and a detection chip, the mounting body is provided with a mounting cavity for placing the detection chip, the mounting body is provided with a liquid inlet hole, and the liquid inlet holes are connected to the mounting cavity, the resistance component is sleeved on the outside of the infusion component, the infusion component is introduced into the mounting cavity through the liquid inlet hole, the resistance component is in resistance with the hole wall of the liquid inlet hole, the infusion component is used to transport sample droplets, and the detection chip is used to detect the sample droplets transported by the infusion component.

[0005] A droplet detection system includes a droplet control device, a first driving member, a second driving member and a sample collection member, wherein the first driving member is connected to the liquid receiving tube, the second driving member is connected to the oil infusion tube, and the sample collection member is connected to the sample infusion tube.

[0006] A droplet detection method, using the droplet detection system, comprises the following steps:

[0007] The first driving member drives the oil in the oil infusion tube to flow toward the detection chip;

[0008] The sample collection tube collects the sample through the sample collection piece, and the sample solution is transported to the position where the sample collection tube and the detection chip are aligned through the sample collection piece;

[0009] The oil in the oil infusion tube and the sample in the sample infusion tube are sucked into the liquid receiving tube by the second driving member, and the sample forms separated droplets in the liquid receiving tube;

[0010] The droplets are detected by a detection chip.

[0011] In one embodiment, the infusion assembly includes a liquid receiving tube, a sample infusion tube and an oil infusion tube. The mounting body is also provided with a liquid receiving hole, the liquid receiving hole is connected with the mounting cavity, the liquid receiving tube extends into the mounting cavity through the liquid receiving hole, the liquid inlet hole includes an oil hole and a sample hole, the sample infusion tube extends into the mounting cavity through the sample hole, the oil infusion tube extends into the mounting cavity through the oil hole, and the liquid receiving tube, the sample infusion tube and the oil infusion tube are all connected in the mounting cavity.

[0012] In one embodiment, the resistance member assembly includes a first resistance member, a second resistance member and a third resistance member, the first resistance member is sleeved on the outside of the liquid receiving tube, and the first resistance member conflicts with the hole wall of the liquid receiving hole, the second resistance member is sleeved on the outside of the sample infusion tube, and the second resistance member conflicts with the hole wall of the sample hole, and the third resistance member is sleeved on the outside of the oil infusion tube, and the third resistance member conflicts with the hole wall of the oil hole.

[0013] In one embodiment, the first resistance member includes a first sealing member, a first pressing ring and a first threaded ring, the first sealing member, the first pressing ring and the first threaded ring are all sleeved on the liquid receiving tube, the first sealing member is used to seal the opening of the liquid receiving hole close to the mounting cavity, and the first sealing member is in contact with the detection chip, the first pressing ring is used to press against the hole wall of the liquid receiving hole, and the first threaded ring is threadedly matched with the hole wall of the liquid receiving hole; and / or the second resistance member includes a second sealing member, a second pressing ring and a second threaded ring, the second sealing member, the second pressing ring and the second threaded ring are all sleeved on the sample infusion tube, and the second sealing member is used to The sample hole is sealed near the opening of the mounting cavity, and the second sealing member is in conflict with the detection chip, the second pressing ring is used to press against the hole wall of the sample hole, and the second threaded ring is threadedly matched with the hole wall of the sample hole; and / or the third conflicting member includes a third sealing member, a third pressing ring and a third threaded ring, the third sealing member, the third pressing ring and the third threaded ring are all sleeved on the oil infusion pipe, the third sealing member is used to seal the oil hole near the opening of the mounting cavity, and the third sealing member is in conflict with the detection chip, the third pressing ring is used to press against the hole wall of the oil hole, and the third threaded ring is threadedly matched with the hole wall of the oil hole.

[0014] In one embodiment, the oil delivery pipe includes an oil delivery base pipe, a first oil delivery branch pipe and a second oil delivery branch pipe, the oil delivery hole includes a first branch hole and a second branch hole, the first branch hole and the second branch hole are both connected to the installation cavity, the first oil delivery branch pipe and the second oil delivery branch pipe are both connected to the oil delivery base pipe, the first oil delivery branch pipe extends into the installation cavity through the first branch hole, the second oil delivery branch pipe extends into the installation cavity through the second branch hole, the first oil delivery branch pipe and the second oil delivery branch pipe are both connected to the sample delivery pipe, and the sample delivery pipe is located between the first oil delivery branch pipe and the second oil delivery branch pipe.

[0015] In one embodiment, the droplet control device further includes a positioning member, and a positioning hole is further provided on the mounting body, the positioning hole is communicated with the mounting cavity, and the positioning member is in positioning contact with the detection chip through the positioning hole.

[0016] In one embodiment, the droplet control device also includes a cover plate, a first optical detection port is opened at the bottom of the mounting body, the first optical detection port corresponds to the detection chip, a second optical detection port is opened on the cover plate, the cover plate is detachably mounted on the top of the mounting body, and the second optical detection port corresponds to the detection chip.

[0017] In one embodiment, the droplet control device further includes a fastener, a first fastening hole corresponding to the fastener is formed on the cover plate, a second fastening hole corresponding to the fastener is formed on the mounting body, and the cover plate is detachably matched with the mounting body through the fastener.

[0018] The above-mentioned droplet control device first determines the infusion inner diameter of the infusion component according to the needs of droplet detection, that is, determines the liquid volume when the sample droplet passes through the detection chip. In addition, the detection chip is placed in the installation cavity to ensure that the sample droplet is detected in the installation cavity, thereby ensuring the detection effect of the detection chip on the sample droplet. When the infusion component is introduced into the installation cavity through the liquid inlet hole, the infusion component is mutually conflicted with the hole wall of the liquid inlet hole through the conflict component, that is, the relative movement of the infusion component in the liquid inlet hole is effectively limited. Compared with the traditional connection method between the infusion tube and the clamp (a rubber pad is arranged on the clamp, and the infusion tube is relatively fixed to the clamp by inserting the rubber pad, but a part of the resilience of the rubber pad will cause the infusion tube to separate from the clamp), after the infusion component and the liquid inlet hole are installed in alignment, the above-mentioned droplet control device has the resistance component and the hole wall of the liquid inlet hole in conflict, that is, the resistance force generated by the resistance component can be fully used to overcome the corresponding movement of the infusion component in the liquid inlet hole, thereby effectively avoiding leakage of the droplet control device and improving the detection effect of the droplet control device on the sample.

[0019] When the above-mentioned microfluidic detection system is in use, the sample collection tube collects the sample solution through the sample collection member. At the same time, the flow capacity of the oil and the sample solution in the droplet control device is ensured by the first driving member and the second driving member. Compared with the traditional connection method of the infusion tube and the clamp (a rubber pad is arranged on the clamp, and the infusion tube is relatively fixed with the clamp by inserting the rubber pad, but a part of the resilience of the rubber pad will cause the infusion tube and the clamp to separate), after the infusion component and the liquid inlet hole of the above-mentioned droplet control device are installed in alignment, the resistance component and the hole wall of the liquid inlet hole are in conflict, that is, the resistance force generated by the resistance component can be fully used to overcome the corresponding movement of the infusion component in the liquid inlet hole and the liquid receiving hole, thereby effectively avoiding leakage of the droplet control device and improving the detection effect of the droplet detection system on the sample.

[0020] When the above-mentioned droplet detection method is used, the first driving member, the second driving member and the sample collection member cooperate to provide power for the flow of oil and sample solution, that is, to ensure the droplet separation effect when the sample solution also passes through the detection chip. At the same time, the resistance component of the droplet control device is in conflict with the hole wall of the liquid inlet hole, that is, the resistance force generated by the resistance component can be fully used to overcome the corresponding movement of the infusion component in the liquid inlet hole and the liquid receiving hole, thereby effectively avoiding leakage of the droplet control device and improving the detection effect of the droplet detection method on the sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a schematic structural diagram of a droplet control device according to an embodiment;

[0022] Figure 2 is a cross-sectional schematic diagram of a droplet control device according to an embodiment;

[0023] Figure 3 is a cross-sectional schematic diagram of a droplet control device according to another embodiment;

[0024] Figure 4 Flow chart of the droplet detection method.

[0025] 100. Installation body, 101. First optical inspection port, 110. Installation cavity, 111. Liquid inlet hole, 112. Liquid receiving hole, 113. Oil hole, 114. Sample hole, 120. Positioning member, 130. Cover plate, 131. Second optical inspection port, 140. Fastener, 200. Infusion assembly, 210. Liquid receiving tube, 220. Sample infusion tube, 230. Oil infusion tube, 231. First oil delivery branch pipe, 232. Second oil delivery branch pipe, 300. Resistance assembly, 310. First resistance member, 311. First sealing member, 312. First pressure ring, 313. First threaded ring, 400. Inspection chip. DETAILED DESCRIPTION

[0026] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0027] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0028] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0029] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0031] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only implementation method.

[0032] like Figures 1 to 3 As shown, in one embodiment, a droplet control device is provided, the droplet control device comprising: a mounting body 100, an infusion component 200, a resistance component 300 and a detection chip 400, the mounting body 100 is provided with a mounting cavity 110 for placing the detection chip 400, the mounting body 100 is provided with a liquid inlet hole 111, and the liquid inlet hole 111 is communicated with the mounting cavity 110, the resistance component 300 is sleeved on the outside of the infusion component 200, the infusion component 200 is introduced into the mounting cavity 110 through the liquid inlet hole 111, the resistance component 300 is in resistance with the hole wall of the liquid inlet hole 111, the infusion component 200 is used to transport sample droplets, and the detection chip 400 is used to detect the sample droplets transported by the infusion component 200.

[0033] The above-mentioned droplet control device first determines the infusion inner diameter of the infusion component 200 according to the needs of droplet detection, that is, determines the liquid volume when the sample droplet passes through the detection chip 400 for detection. In addition, the detection chip 400 is placed in the installation cavity 110 to ensure that the sample droplet is detected in the installation cavity 110, thereby ensuring the detection effect of the detection chip 400 on the sample droplet. After the infusion component 200 is introduced into the installation cavity 110 through the liquid inlet hole 111, the infusion component 200 achieves mutual interference with the hole wall of the liquid inlet hole 111 through the interference component 300, that is, the relative movement of the infusion component 200 in the liquid inlet hole 111 is effectively restricted. Compared with the traditional connection method between the infusion tube and the clamp (a rubber pad is arranged on the clamp, and the infusion tube is relatively fixed to the clamp by inserting the rubber pad, but a part of the resilience of the rubber pad will cause the infusion tube to separate from the clamp), after the infusion component 200 and the liquid inlet hole 111 are installed in alignment, the above-mentioned droplet control device has the resistance component 300 in contact with the hole wall of the liquid inlet hole 111, that is, the resistance force generated by the resistance component 300 can be fully used to overcome the corresponding movement of the infusion component 200 in the liquid inlet hole 111, thereby effectively avoiding leakage of the droplet control device and improving the detection effect of the droplet control device on the sample.

[0034] like Figures 1 to 3As shown, in one embodiment, the infusion assembly 200 includes a liquid receiving tube 210, a sample liquid infusion tube 220 and an oil liquid infusion tube 230, and the installation body 100 is further provided with a liquid receiving hole 112, the liquid receiving hole 112 is connected to the installation cavity 110, the liquid receiving tube 210 extends into the installation cavity 110 through the liquid receiving hole 112, the liquid inlet hole 111 includes an oil hole 113 and a sample hole 114, the sample liquid infusion tube 220 extends into the installation cavity 110 through the sample hole 114, the oil liquid infusion tube 230 extends into the installation cavity 110 through the oil hole 113, and the liquid receiving tube 210, the sample liquid infusion tube 220 and the oil liquid infusion tube 230 are all connected in the installation cavity 110. Specifically, the installation body 100 is a box body or a box body. The liquid receiving hole 112 is located at the first side of the mounting body 100, the oil hole 113 and the sample hole 114 are located at the second side of the mounting body 100, and the first side and the second side are corresponding sides on the mounting body 100. The liquid receiving tube 210 is extended into the mounting cavity 110 through the liquid receiving hole 112, the sample infusion tube 220 is extended into the mounting cavity 110 through the sample hole 114, and the oil infusion tube 230 is extended into the mounting cavity 110 through the oil hole 113. At this time, the sample infusion tube 220 and the oil infusion tube 230 are both connected to the end of the liquid receiving tube 210. By utilizing the incompatibility between the sample solution and the oil, after the sample solution and the oil are mixed, a plurality of mutually separated microdroplets are formed in the liquid receiving tube 210. The detection chip 400 detects each microdroplet, thereby ensuring the detection effect of the microfluidic control device on the sample solution.

[0035] like Figures 1 to 3As shown, in one embodiment, the resistance member assembly includes a first resistance member 310, a second resistance member and a third resistance member, wherein the first resistance member 310 is sleeved on the outside of the liquid receiving tube 210, and the first resistance member 310 conflicts with the hole wall of the liquid receiving hole 112, the second resistance member is sleeved on the outside of the sample infusion tube 220, and the second resistance member conflicts with the hole wall of the sample hole 114, and the third resistance member is sleeved on the outside of the oil infusion tube 230, and the third resistance member conflicts with the hole wall of the oil hole 113. Specifically, the first resistance member 310 conflicts with the liquid receiving hole 112, thereby effectively limiting the displacement of the liquid receiving tube 210 on the installation body 100. The second resistance member conflicts with the hole wall of the sample hole 114, thereby effectively limiting the displacement of the sample infusion tube 220 on the installation body 100. The third abutment member abuts against the wall of the oil hole 113, thereby effectively limiting the displacement of the oil infusion tube 230 on the mounting body 100. Compared with the traditional method (for example, when fixing the sample infusion tube 220 and the oil infusion tube 230, a rubber pad is installed on the mounting body 100, and the sample infusion tube 220 and the oil infusion tube 230 are both inserted into the same rubber pad for elastic abutment), this assembly method of the above-mentioned microfluidic control device can more effectively ensure the assembly effect of each pipe (including the liquid receiving tube 210, the sample infusion tube 220 and the oil infusion tube 230) on the mounting body 100, and it is also convenient for the installation and adjustment of each pipe.

[0036] like Figures 1 to 3As shown, in one embodiment, the first resistance member 310 includes a first sealing member 311, a first pressing ring 312 and a first threaded ring 313, and the first sealing member 311, the first pressing ring 312 and the first threaded ring 313 are all sleeved on the liquid receiving tube 210, the first sealing member 311 is used to seal the opening of the liquid receiving hole 112 close to the installation cavity 110, and the first sealing member 311 is in contact with the detection chip 400, the first pressing ring 312 is used to press against the hole wall of the liquid receiving hole 112, and the first threaded ring 313 is threadedly matched with the hole wall of the liquid receiving hole 112. And / or the second resistance member includes a second sealing member, a second pressing ring and a second threaded ring, the second sealing member, the second pressing ring and the second threaded ring are all sleeved on the sample infusion tube 220, the second sealing member is used to seal the opening of the sample hole 114 close to the installation cavity 110, and the second sealing member is in resistance against the detection chip 400, the second pressing ring is used to press against the hole wall of the sample hole 114, and the second threaded ring is threadedly matched with the hole wall of the sample hole 114. And / or the third resistance member includes a third sealing member, a third pressing ring and a third threaded ring, the third sealing member, the third pressing ring and the third threaded ring are all sleeved on the oil infusion pipe 230, the third sealing member is used to seal the opening of the oil hole 113 close to the installation cavity 110, and the third sealing member is in contact with the detection chip 400, the third pressing ring is used to press against the hole wall of the oil hole 113, and the third threaded ring is threadedly matched with the hole wall of the oil hole 113.

[0037] Specifically, the first sealing member 311 is an elastic block or an elastic ring. When the liquid receiving tube 210 is installed on the installation body 100, the first sealing member 311 blocks the liquid receiving hole 112 close to the opening of the installation cavity 110, and the first sealing member 311 conflicts with the detection chip 400. That is, on the one hand, it can prevent the liquid inside the installation cavity 110 from leaking through the liquid receiving hole 112, and on the other hand, the first sealing member 311 also plays an auxiliary positioning role for the detection chip 400. Further, the first pressing ring 312 conflicts with the inner hole wall of the liquid receiving hole 112, thereby further ensuring the conflict fixing ability of the first conflicting member 310 and the liquid receiving hole 112. Further, the first threaded ring 313 can be directly threadedly matched with the hole wall of the liquid receiving hole 112, so that the first threaded ring 313 can directly block the liquid receiving hole 112 away from the opening of the installation cavity 110 after threading with the hole wall of the liquid receiving hole 112. The first resisting member 310 is simultaneously installed and matched with the liquid receiving hole 112 through the first sealing member 311 , the first pressing ring 312 and the first threaded ring 313 , which effectively ensures the installation and fixing effect of the liquid receiving tube 210 on the installation body 100 .

[0038] The second sealing member is an elastic block or an elastic ring. When the sample infusion tube 220 is installed on the mounting body 100, the second sealing member blocks the sample hole 114 close to the orifice of the mounting cavity 110, and the second sealing member conflicts with the detection chip 400. That is, on the one hand, it can prevent the liquid inside the mounting cavity 110 from leaking through the sample hole 114, and on the other hand, the second sealing member also plays an auxiliary positioning role for the detection chip 400. Further, the second pressing ring conflicts with the inner hole wall of the sample hole 114, thereby further ensuring the conflict fixing ability of the second conflicting member and the sample hole 114. Furthermore, the second threaded ring can be directly threadedly matched with the hole wall of the sample hole 114, so that the second threaded ring can directly block the orifice of the sample hole 114 away from the mounting cavity 110 after threading with the hole wall of the sample hole 114. The second abutment member is simultaneously installed and matched with the sample hole 114 through the second sealing member, the second pressing ring and the second threaded ring, thereby effectively ensuring the installation and fixing effect of the sample infusion tube 220 on the installation body 100 .

[0039] The third sealing member is an elastic block or an elastic ring. When the oil infusion tube 230 is installed on the installation body 100, the third sealing member blocks the oil hole 113 close to the orifice of the installation cavity 110, and the third sealing member conflicts with the detection chip 400. That is, on the one hand, it can prevent the liquid inside the installation cavity 110 from leaking through the oil hole 113, and on the other hand, the third sealing member also plays an auxiliary positioning role for the detection chip 400. Further, the third pressing ring conflicts with the inner hole wall of the oil hole 113, thereby further ensuring the conflict fixing ability of the third conflicting member and the oil hole 113. Furthermore, the third threaded ring can be directly threadedly matched with the hole wall of the oil hole 113, so that the third threaded ring can directly block the oil hole 113 away from the orifice of the installation cavity 110 after threading with the hole wall of the oil hole 113. The third abutment member is simultaneously installed and matched with the oil hole 113 through the third sealing member, the third pressing ring and the third threaded ring, which effectively ensures the installation and fixing effect of the oil infusion tube 230 on the installation body 100.

[0040] like Figures 1 to 3As shown, in one embodiment, the oil delivery pipe 230 includes an oil delivery base pipe, a first oil delivery branch pipe 231 and a second oil delivery branch pipe 232, the oil delivery hole includes a first branch hole and a second branch hole, the first branch hole and the second branch hole are both connected to the installation cavity 110, the first oil delivery branch pipe 231 and the second oil delivery branch pipe 232 are both connected to the oil delivery base pipe, the first oil delivery branch pipe 231 extends into the installation cavity 110 through the first branch hole, the second oil delivery branch pipe 232 extends into the installation cavity 110 through the second branch hole, the first oil delivery branch pipe 231 and the second oil delivery branch pipe 232 are both connected to the sample delivery pipe 220, and the sample delivery pipe 220 is located between the first oil delivery branch pipe 231 and the second oil delivery branch pipe 232. Specifically, the oil in the oil barrel can be introduced into the oil base pipe by suction with a syringe or negative pressure extraction, and then the oil in the oil base pipe is diverted through the first oil branch pipe 231 and the second oil branch pipe 232. At this time, the sample solution in the sample infusion pipe 220 will be separated into droplets under the simultaneous action of the first oil branch pipe 231 and the second oil branch pipe 232 (outputting the oil at the same time). The above implementation method can improve the droplet separation effect of the sample solution.

[0041] like Figures 1 to 3 As shown, in one embodiment, the droplet control device further includes a positioning member 120. A positioning hole is also provided on the mounting body 100, and the positioning hole is connected to the mounting cavity 110, and the positioning member 120 is positioned and interfered with the detection chip 400 through the positioning hole. Specifically, the positioning member 120 is a positioning rod or a positioning bolt. In the above embodiment, it is mentioned that the first sealing ring, the second sealing ring and the third sealing ring and the three components will interfere with the detection chip 400. That is, the interference fixation of the corresponding two sides of the detection chip 400 is achieved, and at this time, the detection chip 400 is fixed with the help of the positioning member 120, thereby further improving the fixing effect of the detection chip 400 in the mounting cavity 110. In addition, by controlling the depth of the positioning member 120 inserted into the mounting cavity 110, the positioning member 120 can drive the detection chip 400 to move accordingly, that is, the position adjustment of the detection chip 400 is achieved.

[0042] like Figures 1 to 3As shown, in one embodiment, the droplet control device further includes a cover plate 130. A first optical detection port 101 is provided at the bottom of the mounting body 100, and the first optical detection port 101 corresponds to the detection chip 400. A second optical detection port 131 is provided on the cover plate 130, and the cover plate 130 is detachably mounted on the top of the mounting body 100, and the second optical detection port 131 corresponds to the detection chip 400. Specifically, the first optical detection port 101 facilitates the optical system to detect sample droplets passing through the detection chip 400 on the one hand, and facilitates the staff to adjust the position of the detection chip 400 according to the refraction or reflection of the light on the detection chip 400 (the light emitted by the optical system). The second optical detection port 131 facilitates the observation of the state of the optical system and the adjustment of the relative position of the detection chip 400 and the optical system.

[0043] like Figures 1 to 3 As shown, in one embodiment, the droplet control device further includes a fastener 140. The cover plate 130 is provided with a first fastening hole corresponding to the fastener 140, and the mounting body 100 is provided with a second fastening hole corresponding to the fastener 140, and the cover plate 130 is detachably matched with the mounting body 100 through the fastener 140. Specifically, the fastener 140 is a fastening bolt or a fastening screw, and the fastener 140 can realize the alignment and fixation of the cover plate 130 and the mounting body 100.

[0044] In one embodiment, a droplet detection system includes the droplet control device described in any one of the above embodiments, and also includes a first driving member, a second driving member and a sample collection member, wherein the first driving member is connected to the liquid receiving tube 210, the second driving member is connected to the oil infusion tube 230, and the sample collection member is connected to the sample infusion tube 220.

[0045] When the microfluidic detection system is in use, the sample collection tube collects the sample solution through the sample collection member. At the same time, the flow capacity of the oil and the sample solution in the droplet control device is ensured by the first driving member and the second driving member. Compared with the traditional connection method of the infusion tube and the clamp (a rubber pad is arranged on the clamp, and the infusion tube is relatively fixed with the clamp by inserting the rubber pad, but a part of the resilience of the rubber pad will cause the infusion tube to separate from the clamp), after the infusion component 200 and the liquid inlet hole 111 are installed in the above droplet control device, the conflict component 300 conflicts with the hole wall of the liquid inlet hole 111, that is, the conflicting force generated by the conflict component 300 can be fully used to overcome the corresponding movement of the infusion component 200 in the liquid inlet hole 111 and the liquid receiving hole 112, thereby effectively avoiding leakage of the droplet control device and improving the detection effect of the droplet detection system on the sample.

[0046] In one embodiment, the first driving member and the second driving member are syringes or negative pressure pumps. The sample collecting member is a pipette or a liquid collection needle.

[0047] like Figure 4 As shown, in one embodiment, a droplet detection method, using the droplet detection system described in the above embodiment, includes the following steps:

[0048] S100, driving the oil in the oil infusion tube 230 to flow toward the detection chip 400 through the first driving member;

[0049] S200, collecting the sample by the sample infusion tube 220 through the sample collection piece, and delivering the sample solution to the position where the sample infusion tube 220 and the detection chip 400 are aligned through the sample collection piece;

[0050] S300, the oil in the oil infusion tube 230 and the sample in the sample infusion tube 220 are sucked into the liquid receiving tube 210 through the second driving member, and the sample forms separated droplets in the liquid receiving tube 210;

[0051] S400 , detecting the droplet through the detection chip 400 .

[0052] When the above-mentioned droplet detection method is used, the first driving member, the second driving member and the sample collection member cooperate to provide power for the flow of oil and sample solution, that is, to ensure the droplet separation effect when the sample solution also passes through the detection chip 400. At the same time, the resistance component 300 of the droplet control device is in conflict with the hole wall of the liquid inlet hole 111, that is, the resistance force generated by the resistance component 300 can be fully used to overcome the corresponding movement of the infusion component 200 in the liquid inlet hole 111 and the liquid receiving hole 112, thereby effectively avoiding leakage of the droplet control device and improving the detection effect of the droplet detection method on the sample.

[0053] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0054] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A droplet control device, characterized in that: The droplet control device comprises: a mounting body, an infusion component, a conflict component, a detection chip and a positioning member, the mounting body is provided with a mounting cavity for placing the detection chip, the mounting body is provided with a liquid inlet hole and a positioning hole, and the liquid inlet hole and the positioning hole are both connected with the mounting cavity, the conflict component is sleeved on the outside of the infusion component, the infusion component is introduced into the mounting cavity through the liquid inlet hole, the conflict component conflicts with the hole wall of the liquid inlet hole, the positioning member conflicts with the detection chip through the positioning hole, the infusion component is used to transport sample droplets, and the detection chip is used to detect the sample droplets transported by the infusion component; Wherein, the infusion component includes a sample infusion tube, the liquid inlet hole includes a sample hole, and the sample infusion tube extends into the installation cavity through the sample hole; the interference component includes a second interference member, the second interference member is sleeved on the outside of the sample infusion tube, and the second interference member is in conflict with the hole wall of the sample hole; the second interference member includes a second sealing member, a second pressure ring and a second threaded ring, the second sealing member, the second pressure ring and the second threaded ring are all sleeved on the sample infusion tube, the second sealing member is used to seal the hole opening of the sample hole close to the installation cavity, and the second sealing member is in conflict with the detection chip, the second pressure ring is used to press against the hole wall of the sample hole, and the second threaded ring is threadedly matched with the hole wall of the sample hole.

2. The droplet control device according to claim 1, characterized in that: The infusion assembly also includes a liquid receiving tube and an oil infusion tube. The mounting body is also provided with a liquid receiving hole, the liquid receiving hole is connected with the mounting cavity, the liquid receiving tube extends into the mounting cavity through the liquid receiving hole, the liquid inlet hole also includes an oil hole, the oil infusion tube extends into the mounting cavity through the oil hole, and the liquid receiving tube, the sample infusion tube and the oil infusion tube are all connected in the mounting cavity.

3. The droplet control device according to claim 2, characterized in that: The resistance member assembly also includes a first resistance member and a third resistance member. The first resistance member is sleeved on the outside of the liquid receiving tube and conflicts with the hole wall of the liquid receiving hole. The third resistance member is sleeved on the outside of the oil infusion tube and conflicts with the hole wall of the oil hole.

4. The droplet control device according to claim 3, characterized in that: The first abutting member includes a first sealing member, a first pressing ring and a first threaded ring. The first sealing member, the first pressing ring and the first threaded ring are all sleeved on the liquid receiving tube. The first sealing member is used to block the opening of the liquid receiving hole close to the installation cavity, and the first sealing member is in conflict with the detection chip. The first pressing ring is used to press against the hole wall of the liquid receiving hole, and the first threaded ring is threadedly matched with the hole wall of the liquid receiving hole. And / or, the third resistance member includes a third sealing member, a third pressure ring and a third threaded ring, and the third sealing member, the third pressure ring and the third threaded ring are all sleeved on the oil infusion pipe, the third sealing member is used to seal the opening of the oil hole close to the installation cavity, and the third sealing member is in conflict with the detection chip, the third pressure ring is used to press against the hole wall of the oil hole, and the third threaded ring is threadedly matched with the hole wall of the oil hole.

5. The droplet control device according to claim 3, characterized in that: The oil delivery pipe includes an oil delivery base pipe, a first oil delivery branch pipe and a second oil delivery branch pipe, the oil hole includes a first branch hole and a second branch hole, the first branch hole and the second branch hole are both connected to the installation cavity, the first oil delivery branch pipe and the second oil delivery branch pipe are both connected to the oil delivery base pipe, the first oil delivery branch pipe extends into the installation cavity through the first branch hole, the second oil delivery branch pipe extends into the installation cavity through the second branch hole, the first oil delivery branch pipe and the second oil delivery branch pipe are both connected to the sample delivery pipe, and the sample delivery pipe is located between the first oil delivery branch pipe and the second oil delivery branch pipe.

6. The droplet control device according to claim 2, characterized in that: It also includes a cover plate, a first optical detection port is opened at the bottom of the mounting body, the first optical detection port corresponds to the detection chip, a second optical detection port is opened on the cover plate, the cover plate is detachably installed on the top of the mounting body, and the second optical detection port corresponds to the detection chip.

7. The droplet control device according to claim 6, characterized in that: It also includes a fastener, the cover plate is provided with a first fastening hole corresponding to the fastener, the mounting body is provided with a second fastening hole corresponding to the fastener, and the cover plate is detachably matched with the mounting body through the fastener.

8. A droplet detection system, comprising the droplet control device according to any one of claims 2 to 6, and further comprising a first driving member, a second driving member and a sample collection member, wherein the first driving member is connected to the liquid receiving tube, the second driving member is connected to the oil infusion tube, and the sample collection member is connected to the sample infusion tube.

9. A droplet detection method, using the droplet detection system of claim 8, characterized in that: The steps include: The first driving member drives the oil in the oil infusion tube to flow toward the detection chip; The sample collection piece enables the sample infusion tube to collect the sample, and the sample solution is transported to the position where the sample infusion tube and the detection chip are aligned through the sample collection piece; The oil in the oil infusion tube and the sample in the sample infusion tube are sucked into the liquid receiving tube by the second driving member, and the sample forms separated droplets in the liquid receiving tube; The droplets are detected by a detection chip.

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