A kind of insecticide composition containing tetrachlorantraniliprole and flupyrazone and application thereof

Abstract

The present invention relates to the field of pesticides and insecticides, and provides a pesticide composition containing tetrachlorantraniliprole and flupyrazone and its application, wherein the pesticide composition comprises tetrachlorantraniliprole and flupyrazone, and the weight ratio of the two active components is 1:80 to 80:1. The composition of the present invention has obvious synergistic effect, the dosage of the agent is greatly reduced compared with the single active ingredient pesticide, and can prevent and control a variety of plant pests, and also has the advantages of reducing pesticide residues and delaying the generation of pest resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides and relates to application of a composition containing tetrachlorantraniliprole and flupyrazone on crop pests. Background Art

[0002] Tetrachlorantraniliprole is a dicarboxamide insecticide containing pyridylpyrazoles. It is the first diamide insecticide product in my country with independent intellectual property rights. It is a nicotinic acid receptor activator. Using chlorantraniliprole as the lead compound, the benzene ring substituents and pyrazole substituents in its structure were modified to find the compound tetrachlorantraniliprole with high insecticidal activity.

[0003] Tetrachlorantraniliprole will affect the growth of larvae and pupae, reduce the weight of the insect body and prolong the development period. It has contact, stomach poison and systemic transmission effects. It has excellent control effects on lepidopteran pests such as the rice stem borer, rice leaf roller, and the diamondback moth, cabbage worm, and beet armyworm on vegetables. It has low toxicity to mammals and has good application prospects and development value. The structural formula is:

[0004]

[0005] Flupyralidone is a neonicotinoid insecticide developed by Bayer, and is also a new type of butenolide compound. It is an insect nicotinic acetylcholine receptor (nAChR) agonist. It acts on the central nervous system of target pests, bonds to receptor proteins, activates receptors to produce biological reactions, induces depolarized ion flow, and puts nerve cells in an excited state. However, unlike acetylcholine, flupyralidone cannot inactivate acetylcholinesterase, resulting in permanent opening of postsynaptic receptors, and ultimately leads to disorders of the insect nervous system and subsequent collapse.

[0006] Flupyralidone belongs to the class of butene carboxylic acid lactone insecticides and is a nicotinic acetylcholine receptor agonist. It is mainly used to control piercing-sucking mouthparts pests, such as aphids, whiteflies, psyllids, leafhoppers, scale insects, beetles, leafminers, mealybugs, soft scales, citrus psyllids, weevils and thrips, etc. It can quickly stop pests from feeding and can effectively control vector pests that spread viruses and bacteria. Due to its penetrating effect, it is also effective against hidden pests.

[0007] Since crops often suffer from multiple insect pests, and most pesticides can only control one type or category of pests, the mixing of two or more pesticides with different mechanisms of action can achieve the effect of controlling different pests; it can also improve the control effect and rapidity of the pesticides, thereby increasing the efficacy and reducing drug resistance; it can also improve efficiency and reduce drug costs.

[0008] The combination of tetrachlorantraniliprole and flupyrazone can increase the target of action and reduce the amount of pesticide used, which plays an important role in delaying pest resistance and reducing environmental pressure. In addition, in the prior art, the technical solution of the composition containing tetrachlorantraniliprole and flupyrazone has not been specifically disclosed. Summary of the invention

[0009] The present invention aims to provide an insecticidal composition containing tetrachlorantraniliprole and flupyrazone, which has a significant synergistic effect, expands the insecticidal spectrum, can reduce the amount of medicine used during use, reduce the pesticide residue on crops, delay pest resistance, and extend the service life of pesticides, thus saving time, labor and money for farmers.

[0010] The technical feature of the composition of the present invention is: an insecticidal composition containing tetrachlorantraniliprole and flupyrazone, wherein the weight ratio of tetrachlorantraniliprole to flupyrazone is 1:80 to 80:1. Preferably, the weight ratio of tetrachlorantraniliprole to flupyrazone is 1:10 to 20:1; preferably, the weight ratio of tetrachlorantraniliprole to flupyrazone is 1:5 to 10:1; preferably, the weight ratio of tetrachlorantraniliprole to flupyrazone is 1:1.

[0011] The weight percentage of the active component in the composition of the present invention is between 0.5% and 95%; preferably, the weight percentage of the active component in the composition is between 1% and 85%.

[0012] An insecticide composition containing tetrachlorantraniliprole and flupyrazone, the insecticide composition also includes an adjuvant, and the adjuvant includes a carrier and an adjuvant;

[0013] The carrier is one or more of water, solvent or filler;

[0014] Furthermore, the water is deionized water; the solvent is one or more of N,N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate, and acetonitrile; and the filler is one or more of kaolin, diatomaceous earth, bentonite, white carbon black, starch, and light calcium carbonate.

[0015] The auxiliary agent is selected from one or more of antifreeze, dispersant, thickener, stabilizer, disintegrant, wetting agent, aqueous emulsion, defoamer, binder, etc.;

[0016] The insecticide composition of the present invention can be prepared into any dosage form suitable for use in agriculture; the dosage form includes any one of powder, dispersible tablet, granule, soluble powder, soluble granule, soluble tablet, water dispersible granule, wettable powder, microcapsule granule, water dispersible tablet, microcapsule suspension, dispersible liquid, emulsifiable concentrate, emulsifiable concentrate, microemulsion, suspension, suspoemulsion, soluble liquid, and ultra-low volume liquid;

[0017] Furthermore, the formulation is in the form of a suspension, water-dispersible granules, aqueous emulsion, wettable powder, granules or dispersible oil suspension.

[0018] The composition of the present invention is prepared into a suspension, and the components and contents thereof are preferably:

[0019]

[0020] The composition of the present invention is prepared into water dispersible granules, and the components and contents thereof are preferably:

[0021]

[0022] The composition of the present invention is prepared into an aqueous emulsion, and the components and contents thereof are preferably:

[0023]

[0024] The composition of the present invention is prepared into a wettable powder, and the components and contents thereof are preferably:

[0025]

[0026] The composition of the present invention is prepared into granules, and the components and contents thereof are preferably:

[0027]

[0028] The composition of the present invention is prepared into a dispersible oil suspension, and the components and contents thereof are preferably:

[0029]

[0030]

[0031] The present invention also provides the use of the above insecticide composition containing tetrachlorantraniliprole and flupyrazone in preventing and controlling plant pests in agriculture, forestry and gardening.

[0032] Furthermore, the plant pests are mainly chewing mouthparts pests and piercing-sucking mouthparts pests;

[0033] Furthermore, the chewing mouthparts pest is Spodoptera exigua; and the piercing-sucking mouthparts pest is aphid.

[0034] The beet armyworm belongs to the family Noctuidae in the order Lepidoptera. It is a omnivorous pest with a worldwide distribution, intermittent outbreaks, and mainly harms vegetables. The beet armyworm is harmful to vegetables such as green onions, cabbage, Chinese cabbage, celery, cauliflower, carrots, asparagus, water spinach, amaranth, peppers, cowpeas, cauliflower, eggplant, kale, tomatoes, Chinese cabbage, Chinese cabbage, broccoli, spinach, and radishes. The insect is active at night and is good at hiding in secluded places to feed, making it difficult to control. The first-instar larvae gather on the back of the leaves to spin silk webs and have a small appetite. After the third instar, they become scattered and their appetite increases dramatically, causing serious damage to vegetables.

[0035] Both adults and nymphs of aphids suck the sap of host plants, often concentrating on leaves, causing them to shrink and turn yellow, leading to sooty mold; aphids sucking plant sap not only causes plants to lack vitality, but their saliva is also toxic to plants. Aphids are known as the fastest-reproducing insects, and can reproduce 10-30 generations a year, with prominent overlapping generations.

[0036] The present invention also provides the use of the above-mentioned insecticide composition containing tetrachlorantraniliprole and flupyrazone, wherein the plant pests are pests on cruciferous vegetables; further, the cruciferous vegetables are cabbages.

[0037] The present invention also provides an application method of the insecticide composition containing tetrachlorantraniliprole and flupyrazone, wherein the insecticide composition is applied to the pests to be controlled or the medium in which they grow in an effective dose.

[0038] The frequent and extensive use of pesticides has put pests under strong selection pressure. Coupled with the rapid reproduction of pests, their resistance level to conventional pesticides has become increasingly high.

[0039] The insecticidal composition of the present invention has the following advantages:

[0040] (1) The composition of the present invention exhibits a synergistic effect within a certain ratio range, thereby improving the control effect of the composition on pests;

[0041] (2) The effective ingredients in the composition of the present invention have different control targets, which can expand the insecticide range and effectively reduce production costs;

[0042] (3) The two active components in the composition have different mechanisms of action, which effectively delays the development of insecticide resistance and prolongs the service life of the product;

[0043] (4) The two active components in the insecticide composition are used in combination within an appropriate ratio, thereby improving the control effect on pests. DETAILED DESCRIPTION

[0044] The present invention is further described below with reference to the examples. The percentages in the examples are all percentages by weight, but the present invention is not limited thereto.

[0045] Example 1: 30% tetrachlorantraniliprole·flupyrazone suspension (1:1)

[0046] Formula: 15% tetrachlorantraniliprole, 15% flupyrazone, 2% sodium alkyl sulfate, 3% alkylphenol formaldehyde resin polyoxyethylene ether, 0.4% carboxymethyl cellulose, 5% propylene glycol, 0.2% isothiazolinone, and deionized water to make up the balance;

[0047] Preparation method: According to the formula ratio, the active ingredients tetrachlorantraniliprole, flupyrazone and auxiliary agents are placed in a reaction kettle in sequence, water is added to mix evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenous filtration.

[0048] Example 2: 25% tetrachlorantraniliprole·flupyrazone suspension (3:2)

[0049] Formula: 15% tetrachlorantraniliprole, 10% fluopyrazone, 10% dodecyl polyoxyethylene ether phosphate, 2% sodium alkyl polyoxyethylene ether sulfonate, 0.4% magnesium aluminum silicate, 0.2% carboxyethyl cellulose, 4% sorbitol, 0.1% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0050] Preparation method: Same as Example 1.

[0051] Example 3: 15% tetrachlorantraniliprole·flupyrazone suspension (1:2)

[0052] Formula: 5% tetrachlorantraniliprole, 10% fluopyrazone, 8% dodecyl polyoxyethylene ether phosphate, 2% sodium alkyl polyoxyethylene ether sulfonate, 0.4% xanthan gum, 0.1% carboxyethyl cellulose, 4% sorbitol, 0.1% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0053] Preparation method: Same as Example 1.

[0054] Example 4: 20% tetrachlorantraniliprole·flupyrazone water dispersible granules (1:1)

[0055] Formula: 10% tetrachlorantraniliprole, 10% flupyrazone, 4% lignin sulfonate, 3% sodium dodecylbenzene sulfonate, 5% aluminum chloride, and white carbon black as the remainder.

[0056] Preparation method: According to the formula ratio of the embodiment, the active ingredients tetrachlorantraniliprole and flupyrazone are added to a carrier, and other functional additives are added thereto, mixed, and 10-25% of water is added after air flow crushing; then the crushed powder is sprayed with water, granulated, and dried in a boiling granulator, and then sieved to obtain the product.

[0057] Example 5: 40% tetrachlorantraniliprole·flupyrazone water dispersible granules (1:1)

[0058] Formula: 20% tetrachlorantraniliprole, 20% fluopyrazone, 5% lignin sulfonate, 3% BX powder, 5% aluminum chloride, and kaolin as the balance.

[0059] Preparation method: Same as Example 4.

[0060] Example 6: 20% tetrachlorantraniliprole·flupyrazone water dispersible granules (3:1)

[0061] Formula: 15% tetrachlorantraniliprole, 5% fluopyrazone, 5% naphthalenesulfonic acid formaldehyde condensate sodium salt, 4% BX powder, 8% aluminum chloride, and diatomaceous earth as the balance.

[0062] Preparation method: Same as Example 4.

[0063] Example 7: 25% tetrachlorantraniliprole·flupyrazone aqueous emulsion (2:3)

[0064] Formula: 10% tetrachlorantraniliprole, 15% flupyrazone, 12% phosphate, 8% propylene glycol, and deionized water to make up the balance;

[0065] Preparation method: according to the formula ratio of the embodiment, the active ingredient tetrachlorantraniliprole, flupyrazone and emulsifier are added together to dissolve into a uniform oil phase; deionized water and antifreeze are mixed together to form a uniform water phase; the oil phase is added to the water phase while stirring at a high speed in a reactor, water is slowly added until the phase transition point is reached, a shearing machine is turned on for high-speed shearing, and the remaining water is added, and shearing is performed for 30 minutes to form a water emulsion.

[0066] Example 8: 20% tetrachlorantraniliprole·flupyrazone aqueous emulsion (1:3)

[0067] Formula: 5% tetrachlorantraniliprole, 15% flupyrazone, 3% agricultural milk 1601#, 7.5% ethylene glycol, and deionized water to make up the balance;

[0068] Preparation method: Same as Example 7.

[0069] Example 9: 15% tetrachlorantraniliprole·flupyrazone aqueous emulsion (2:1)

[0070] Formula: 10% tetrachlorantraniliprole, 5% flupyrazone, 6% phosphate, 7% ethylene glycol, and deionized water to make up the balance;

[0071] Preparation method: Same as Example 7.

[0072] Example 10: 0.9% tetrachlorvinamide·flupyrazone granules (5:4)

[0073] Formula: 0.5% tetrachlorantraniliprole, 0.4% flupyrazone, 4% pull-opening powder BX, 3% wetting and penetrating agent F, 13% lignin sulfonate, and light calcium carbonate as the balance;

[0074] Preparation method: The active ingredient tetrachlorantraniliprole is weighed and mixed with flupyrazone, a binder and a carrier in a required proportion, the mixture is crushed and mixed with the carrier, the binder is added, stirred and granulated, and then packaged to obtain the product.

[0075] Example 11: 1% tetrachlorantraniliprole·flupyrazone granules (1:1)

[0076] Formula: 0.5% tetrachlorantraniliprole, 0.5% flupyrazone, 10% pull open powder BX, 12% wetting and penetrating agent F, 2% polycarboxylate, bentonite to make up the rest;

[0077] Preparation method: Same as Example 10.

[0078] Example 12: 3% tetrachlorantraniliprole·flupyrazone granules (1:1)

[0079] Formula: 1.5% tetrachlorantraniliprole, 1.5% flupyrazone, 13% sodium dodecylbenzene sulfonate, 1% saponin powder, 13% lignin sulfonate, kaolin as the balance;

[0080] Preparation method: Same as Example 10.

[0081] Example 13: 36% tetrachlorantraniliprole·flupyrazone wettable powder (1:5)

[0082] Formula: 6% tetrachlorantraniliprole, 30% flupyrazone, 7% polycarboxylate, 4% sodium dodecylbenzene sulfonate, kaolin as the balance;

[0083] Preparation method: According to the formula ratio of the embodiment, the active ingredients tetrachlorantraniliprole and flupyrazone are added to the carrier, and other functional adjuvants are added thereto, mixed, and then air flow pulverized and mixed again to obtain a wettable powder.

[0084] Example 14: 22% tetrachlorantraniliprole·flupyrazone wettable powder (10:1)

[0085] Formula: 20% tetrachlorantraniliprole, 2% flupyrazone, 1% fatty amine polyoxyethylene ether, 3% sodium dodecylbenzene sulfonate, kaolin as the balance;

[0086] Preparation method: Same as Example 13.

[0087] Example 15: 30% tetrachlorantraniliprole·flupyrazone wettable powder (1:2)

[0088] Formula: 10% tetrachlorantraniliprole, 20% flupyrazone, 5% alkylphenol polyoxyethylene ether, 3% wetting and penetrating agent F, diatomaceous earth as a supplement;

[0089] Preparation method: Same as Example 13.

[0090] Example 16: 20% tetrachlorantraniliprole·flupyrazone dispersible oil suspension (1:3)

[0091] Formula: 5% tetrachlorantraniliprole, 15% flupyrazone, 2% Nongru 700#, 5% Nongru S-80, 10% methyl oleate, 3% white carbon black, 1% Nongru T-20, and soybean oil as the balance;

[0092] Preparation method: According to the formula ratio, the active ingredient tetrachlorantraniliprole, flupyrazone and other functional additives are placed in a reaction kettle in sequence, oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand grinding, and finally homogenized filtration.

[0093] Example 17: 25% tetrachlorantraniliprole·flupyrazone dispersible oil suspension (2:3)

[0094] Formula: 10% tetrachlorantraniliprole, 15% flupyralidone, 1% Nongru S-80, 2% Nongru 700#, 5% Nongru 0203B, 10% methyl oleate, 3% white carbon black, 1% Nongru T-20, and soybean oil as the balance;

[0095] Preparation method: Same as Example 16.

[0096] Example 18: 40% tetrachlorantraniliprole·flupyrazone dispersible oil suspension (1:1)

[0097] Formula: 20% tetrachlorantraniliprole, 20% flupyrazone, 6% Nongru 0201B, 2% Nongru 700#, 5% Nongru S-80, 10% methyl oleate, 3% white carbon black, 1% Nongru T-20, and soybean oil as the balance;

[0098] Preparation method: Same as Example 16.

[0099] Indoor biological activity assay

[0100] The embodiment of the present invention adopts a method combining indoor toxicity determination and field test. First, through indoor toxicity determination, the co-toxicity coefficient (CTC) of two agents after compounding in a certain ratio is determined. CTC < 80 is antagonistic, CTC > 120 is synergistic, and between 80 and 120 is additive. Field test is carried out on this basis.

[0101] Example 19: The combined toxicity test of tetrachlorantraniliprole and flupyrazone against cabbage beet armyworm was carried out in accordance with NY / T1154.14-2008 "Guidelines for Indoor Organic Testing of Pesticides Part 14: Leaf Dipping Method".

[0102] Test pests: beet armyworm Spodoptera exigua (Hiibner, 1808), third-instar larvae raised uniformly indoors.

[0103] Experimental agents: 95% tetrachlorfenapyr technical and 96% flupyralidone technical, both provided by the R&D center of Hailier Pharmaceutical Group Co., Ltd.

[0104] Drug preparation: Dissolve the original drug in DMF to prepare high-concentration mother liquors, and then dilute the mother liquors with a 0.1% Tween 80 aqueous solution. Set 5 different concentrations for each treatment and keep them for later use.

[0105] Experimental repetition: 80 heads were treated at each concentration, divided into 4 repetitions, and a treatment without drug (including all organic solvents and emulsifiers) was set as a blank control.

[0106] Chemical treatment: Take fresh cabbage leaf discs, immerse them in different concentrations of chemical solution for 10 seconds, take them out, absorb the excess solution, dry them naturally, and then place them in a 12 cm diameter culture dish for culture. Pick 20 3rd-instar beet armyworm larvae in each culture dish, and repeat each treatment 4 times.

[0107] Feeding and observation: The treated test insects were placed in a temperature of 25°C, a humidity of 60% to 70%, and a photoperiod of L:D = 16h:8h for feeding and observation. 48 hours after treatment, the number of dead insects on each leaf was checked.

[0108] Data statistics and analysis:

[0109] According to the survey data, the adjusted mortality rate of each treatment was calculated in percentage (%). The calculation formula is as follows:

[0110]

[0111]

[0112] LC 50 The drug with a relatively small value is the standard drug, and its toxicity index TI A is 100.

[0113] Active ingredient B

[0114] Toxicity index of mixture

[0115] The theoretical toxicity of a mixture is TTI = TIA ×P A +TI B ×P B

[0116] Co-toxicity coefficient

[0117] Where: P A , P B are the proportions of active ingredients A and B in the composition, respectively.

[0118] Among them, A is tetrachlorfenapyr and B is flupyrazone.

[0119] If the blank control mortality rate is less than 5%, no correction is required; if the blank control mortality rate is 5% to 10%, a correction mortality rate is required; if the blank control mortality rate is above 10%, the experiment needs to be repeated.

[0120] Test results and discussion:

[0121] Table 1 The results of the toxicity test of tetrachlorantraniliprole and flupyrazone against Spodoptera exigua

[0122]

[0123]

[0124] As can be seen from Table 1, the mixture of tetrachlorantraniliprole and flupyrazone has a good combined effect on beet armyworm. When the weight ratio of tetrachlorantraniliprole to flupyrazone is 1:80-80:1, the co-toxicity coefficient is greater than 80, showing an additive or synergistic effect; among them, when the weight ratio of tetrachlorantraniliprole to flupyrazone is 1:10-20:1, the co-toxicity coefficient is greater than 120, when the weight ratio of tetrachlorantraniliprole to flupyrazone is 1:5-5:1, the co-toxicity coefficient is greater than 140, and when the weight ratio of tetrachlorantraniliprole to flupyrazone is 1:1, the co-toxicity coefficient is 191.06, and the synergistic effect is most obvious. This shows that the mixture of tetrachlorantraniliprole and flupyrazone to control beet armyworm is reasonable and feasible.

[0125] Example 20: Determination of the combined toxicity of tetrachlorantraniliprole and flupyrazone to cabbage aphids

[0126] The test was conducted in accordance with NY / T1154.6-2013 “Guidelines for Indoor Organic Testing of Pesticides Part 6: Insect Immersion Method”.

[0127] Test pests: Cabbage aphid Brevicoryne brassicae (Linnaeus), which were raised for multiple generations in Xichenghui greenhouse in Chengyang District, Qingdao City, Shandong Province, and sensitive test insects of the same age were selected.

[0128] Experimental agents: 95% tetrachlorfenapyr technical and 96% flupyralidone technical, both provided by the R&D center of Hailier Pharmaceutical Group Co., Ltd.

[0129] Preparation of drugs: Dissolve the original drugs with acetone to prepare high-concentration mother solutions, and then dilute the mother solutions with 0.1% Tween 80 aqueous solution. Set 5 different concentrations for each treatment and keep them for later use.

[0130] Chemical treatment: Cut off the cabbage leaves with uniformly developed second-instar nymphs, immerse them in different concentrations of chemical solutions for 10 seconds, take them out, absorb the excess chemical solution with filter paper, and transfer the test insects to normal conditions for breeding.

[0131] Test repetition: 20 insects were immersed in each repetition, and the number of repetitions was 4 times. The treatment with the corresponding organic solvent without the drug was set as the control. 48 hours after the treatment, the death of the test insects was investigated, and the total number of insects and the number of dead insects were recorded.

[0132] Feeding and observation: The treated test insects were placed in a temperature of 25°C, a humidity of 60% to 70%, and a photoperiod of L:D = 16h:8h for feeding and observation. 48h after treatment, the number of dead insects in each treatment was recorded.

[0133] Data statistics and analysis:

[0134] According to the survey data, the adjusted mortality rate of each treatment was calculated in percentage (%). The calculation formula is as follows:

[0135]

[0136]

[0137] LC 50 The drug with a relatively small value is the standard drug, and its toxicity index TI B is 100.

[0138] Active ingredient A

[0139] Toxicity index of mixture

[0140] The theoretical toxicity of a mixture is TTI = TI A ×P A +TI B ×P B

[0141] Co-toxicity coefficient

[0142] Where: P A , P B are the proportions of active ingredients A and B in the composition, respectively.

[0143] Among them, A is tetrachlorfenapyr and B is flupyrazone.

[0144] If the blank control mortality rate is less than 5%, no correction is required; if the blank control mortality rate is 5%-10%, correction mortality rate is required; if the blank control mortality rate is above 10%, the experiment needs to be repeated.

[0145] Experimental results and discussion:

[0146] Table 2 The results of toxicity test of tetrachlorantraniliprole and flupyrazone on cabbage aphid

[0147]

[0148] As can be seen from Table 2, when tetrachlorantraniliprole and flupyrazone are mixed in the range of 1:20-20:1, the co-toxicity coefficient (CTC) is greater than 80, showing an additive or synergistic effect on cabbage aphids; when tetrachlorantraniliprole and flupyrazone are mixed in the range of 1:5-10:1, the co-toxicity coefficient (CTC) is above 140; when tetrachlorantraniliprole and flupyrazone are mixed in the range of 1:3-5:1, the synergistic effect is more significant, and the co-toxicity coefficient (CTC) is above 160; when tetrachlorantraniliprole and flupyrazone are mixed in the range of 1:1, the synergistic effect is most significant, LC 50 The concentration of tetrachlorantraniliprole and flupyrazone was 0.3313 mg / L, and the co-toxicity coefficient was 196.49, indicating that the mixing of tetrachlorantraniliprole and flupyrazone is reasonable and feasible.

[0149] Field efficacy examples

[0150] Example 21: Field test of the efficacy of a mixture of tetrachlorantraniliprole and flupyrazone against cabbage beet armyworm

[0151] Test pest: beet armyworm Spodoptera exigua (Hübner, 1808).

[0152] Experimental crops: Cruciferous vegetables / cabbage, variety name: “Zhonggan No. 11”.

[0153] Test location: Nancun Town, Pingdu, Qingdao

[0154] Test time: June 20, 2019

[0155] Experimental environment: The soil in the experimental field is uniform, with medium to high fertility, flat terrain, good tillage, and the degree of damage to the cabbage is consistent.

[0156] Test agents: 20% tetrachlorantraniliprole·flupyrazone suspension (1:1), 20% tetrachlorantraniliprole·flupyrazone water dispersible granules (3:1), 10% tetrachlorantraniliprole suspension, 17% flupyrazone soluble. The test set up 9 treatments, each with 4 replicates, a total of 36 plots, all of which were provided by Hailier R&D Center.

[0157] Test basis: GB—T17980.13-2000 "Guidelines for field efficacy tests of pesticides Part 13: Insecticides for controlling lepidopteran larvae of cruciferous vegetables".

[0158] Test method: The agent is mixed with water (900kg / hm 2 ) After diluting to form a uniform solution, use a backpack sprayer to evenly spray the cabbage plants from top to bottom, applying the medicine once.

[0159] Survey method: Sampling was carried out at 5 points in the plot, with 8 plants at each point, and a total of 40 cabbage plants were surveyed in each plot. The insect population base was investigated before spraying, and the number of cabbage and beet plants surviving at night was investigated 3d, 7d, and 14d after spraying.

[0160] Calculation formula and data analysis: Statistics of insect population reduction rate and average control effect of pesticide treatment.

[0161] The formula for calculating the control effect is as follows:

[0162]

[0163] Where:

[0164] PT: control effect, %;

[0165] PT0: number of live insects in the treatment area before application of pesticides, in units of heads;

[0166] PT1: number of live insects in the treatment area after application of pesticides, in units of heads;

[0167] CK0: number of live insects in the blank control area before application, in units of heads;

[0168] CK1: The number of live insects in the blank control area after application of pesticides, in heads.

[0169] The calculation results were retained to two decimal places, and data were statistically analyzed using Excel software and the DPS data processing system was used for difference significance analysis.

[0170] Table 3 Field efficacy of tetrachlorantraniliprole and chlorfenapyr mixed to control cabbage beet armyworm

[0171]

[0172] Note: The above insect population and control efficacy are the average values ​​of 4 repetitions, and the values ​​are rounded to two decimal places.

[0173] The test results are shown in Table 3. 20% tetrachlorantraniliprole·flupyrazone suspension (1:1) and 20% tetrachlorantraniliprole·flupyrazone water dispersible granules (3:1) have good control effects on cabbage armyworm. The minimum dosage of the two compound pesticides 20% tetrachlorantraniliprole·flupyrazone suspension (1:1) and 20% tetrachlorantraniliprole·flupyrazone water dispersible granules (3:1) was 40 g ai / hm3 3 days after the application. 2 、35g ai / hm 2 The control effects were 60.09% and 64.85% respectively, which were better than the two control agents, 10% tetrachlorantraniliprole suspension and 17% flupyrazone soluble solution. The control effects of the two mixed agents, 20% tetrachlorantraniliprole·chlorfenapyr suspension (1:1) and 20% tetrachlorantraniliprole·flupyrazone water dispersible granules (3:1), were above 80.26% 14 days after application, showing good persistence. From the above data, it can be seen that the mixture of tetrachlorantraniliprole and flupyrazone has a high control effect on beet armyworm.

[0174] Example 22: Field test of the efficacy of a mixture of tetrachlorantraniliprole and flupyrazone against cabbage aphids

[0175] Test crops and objects: Cabbage aphid Brevicoryne brassicae (Linnaeus).

[0176] Experimental environment: The degree of damage to cabbage was consistent, the cultivation conditions of all experimental plots were uniform, and the same fertilizer and water management level was adopted.

[0177] Experimental agents: 20% tetrachlorantraniliprole·flupyrazone suspension (1:1), 20% tetrachlorantraniliprole·flupyrazone water dispersible granules (3:1), 10% tetrachlorantraniliprole suspension, 17% flupyrazone soluble agent. The above agents are provided by Hailier R&D Center.

[0178] Test basis: GB—T17980.13-2000 "Guidelines for Field Efficacy Tests of Pesticides Part 27: Insecticides for Control of Aphids on Cruciferous Vegetables".

[0179] Test method: Start applying the pesticide when the number of cabbage aphids is not less than 20, and apply the pesticide once.

[0180] Survey method: Investigate the insect population base before applying the pesticide, and investigate the number of surviving aphids 1 day, 3 days, and 7 days after applying the pesticide.

[0181] Calculation formula and data analysis:

[0182] The formula for calculating the control effect is as follows:

[0183]

[0184] Where:

[0185] PT: control effect, %;

[0186] PT0: number of live insects in the treatment area before application of pesticides, in units of heads;

[0187] PT1: number of live insects in the treatment area after application of pesticides, in units of heads;

[0188] CK0: number of live insects in the blank control area before application, in units of heads;

[0189] CK1: The number of live insects in the blank control area after application of pesticides, in heads.

[0190] The calculation result shall be rounded to two decimal places.

[0191] Table 2 Field efficacy of tetrachlorantraniliprole and flupyrazone mixed to control cabbage aphids

[0192]

[0193]

[0194] Note: The above data are the average of 4 repetitions, and the values ​​are rounded to two decimal places.

[0195] The test results show that the control effects of 20% tetrachlorantraniliprole·flupyrazone suspension (1:1) and 20% tetrachlorantraniliprole·flupyrazone water dispersible granules (3:1) on cabbage aphids are higher than those of a single agent 1 day, 3 days and 7 days after application, and the duration of efficacy is significantly better than that of a single agent; the control effect of 20% tetrachlorantraniliprole·flupyrazone suspension (1:1) on cabbage aphids is slightly better than that of 20% tetrachlorantraniliprole·flupyrazone water dispersible granules (3:1). 20% tetrachlorantraniliprole·flupyrazone suspension (1:1) is slightly better than that of 20% tetrachlorantraniliprole·flupyrazone water dispersible granules (3:1) 7 days after application, and the dosage is 60g ai / hm 2 、50ga.i / hm 2 、40g ai / hm 2 The protective effects were 92.78%, 87.28% and 86.63% respectively.

[0196] Through indoor toxicity determination and field tests, the pesticide composition compounded with tetrachlorantraniliprole and flupyrazone of the present invention showed good control effect on beet armyworm of Lepidoptera and aphids of Homoptera. The pesticide composition or its preparation obtained by compounding of the present invention has significant control effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging the lasting effect. In addition, no phytotoxicity of the compound agent to crops was found in the test, indicating that when the insecticide synergistic synergy of the obtained pesticide composition or preparation is improved, the production cost and use cost can be reduced, and it is safe for crops.

Claims

1. An insecticidal composition containing tetrachlorantraniliprole and flupyrazone, characterized in that: The insecticide composition comprises an active component A and an active component B, wherein the active component A is tetrachlorantraniliprole, the active component B is flupyrazone, and the weight ratio of the tetrachlorantraniliprole to flupyrazone is 1:5 to 10:

1.

2. The insecticidal composition according to claim 1, characterized in that: The weight ratio of tetrachlorantraniliprole to flupyrazone is 1:5, 1:3, 1:1, 3:1, 5:1 and 10:

1.

3. The insecticidal composition according to claim 1, characterized in that: Based on the total mass of the insecticide composition being 100wt%, the sum of the contents of tetrachlorantraniliprole and flupyrazone in the insecticide composition is 1% to 85%.

4. The insecticidal composition according to claim 1, characterized in that: The insecticide composition further comprises an adjuvant, and the adjuvant comprises a carrier and an adjuvant.

5. The insecticidal composition according to claim 1, characterized in that: The insecticide composition can be prepared into any dosage form suitable for use in agriculture.

6. The insecticidal composition according to claim 5, characterized in that: The dosage form is any one of a suspension, a water-dispersible granule, an aqueous emulsion, a wettable powder, a granule or a dispersible oil suspension.

7. Use of the insecticide composition according to claim 1 in controlling plant pests in agriculture, forestry and horticulture, wherein the plant pests are beet armyworm and aphids on cruciferous vegetables cabbage.

8. The use according to claim 7, characterized in that: The insecticidal composition is applied to the pests to be controlled or the medium where they grow in an effective dose.

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