An integrated mechanism for pre-processing steps of chemiluminescence instrument magnetic bead detection

Abstract

The application discloses a chemical luminescence instrument magnetic bead detection pretreatment step integrated mechanism, which comprises a mechanism mounting plate, a crank is rotationally connected to the upper surface of an incubation shaking module mounting plate, a driving motor is fixedly installed on the lower surface of the incubation shaking module mounting plate, a belt is rotationally connected to the output end of the driving motor, the outer surface wall of the crank is slidably connected with the outer surface wall of the belt, an incubation box is rotationally connected to the upper surface of the crank, and a sliding block is fixedly installed on the lower surface of the incubation box. In the application, after the sample to be detected is put into the incubation box, the incubation box starts heating and keeps a constant temperature, the crank starts rotating and drives the incubation box to reciprocatingly shake under the cooperation of the sliding block, the sample to be detected in the detection cup is shaken uniformly while still keeping a constant temperature, the incubation and shaking steps are prevented from interfering with each other, and the use effect of the chemical luminescence instrument is improved.

Description

Technical Field

[0001] The present invention relates to the field of medical instruments, and in particular to an integrated mechanism for pre-processing steps of magnetic bead detection using a chemiluminescence instrument. Background Art

[0002] Chemiluminescence is a method of combining antigens, antibodies, and samples, and then capturing the resulting reactants with magnetic beads. A luminescence promoter is then added to increase the speed and intensity of the reactants' self-luminescence. The number of photons is measured with a luminescence signal meter, and diagnosis is based on this.

[0003] At present, the existing chemiluminescence analyzers still have shortcomings. First, most chemiluminescence analyzers have two steps of incubation and mixing, which causes the sample to precipitate again during incubation after shaking or causes the temperature of the sample to drop during shaking, reducing the use effect of the chemiluminescence analyzer; second, most chemiluminescence analyzers also have separate steps for adding detergents or absorbing waste liquids, which makes the washing process time-consuming and reduces the detection efficiency of the chemiluminescence analyzer. Summary of the Invention

[0004] The purpose of the present invention is to solve the above-mentioned problems of chemiluminescence instrument and propose an integrated mechanism for magnetic bead detection pretreatment steps of chemiluminescence instrument.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A chemiluminescence instrument magnetic bead detection pretreatment step integrated mechanism, including a mechanism mounting plate, an incubation and shaking module mounting plate fixedly mounted on the vertical end of the mechanism mounting plate, the upper surface of the incubation and shaking module mounting plate rotatably connected to a crank, the lower surface of the incubation and shaking module mounting plate fixedly mounted to a drive motor, the output end of the drive motor rotatably connected to a belt, the outer wall of the crank is slidably connected to the outer wall of the belt, the upper surface of the crank is rotatably connected to an incubation box, the inner wall of the incubation box is slidably connected to multiple groups of detection cups, and a slider is fixedly mounted on the lower surface of the incubation box, the lower surface of the slider is slidably connected to the upper surface of the incubation and shaking module mounting plate.

[0007] As a further description of the above technical solution:

[0008] The vertical end of the mechanism mounting plate is fixedly installed with an integrated grabbing and injection mechanism, and the integrated grabbing and injection mechanism includes two groups of guide rail seats, and the two groups of guide rail seats are respectively rotatably connected to the first guide rail and the second guide rail through the transmission wheel, and the outer wall of the second guide rail is fixedly installed with a robotic arm driving motor through a hook, and the output end of the robotic arm driving motor is rotatably connected to the driving guide rail, and the outer wall of the driving guide rail is fixedly installed with a connecting rod, and the outer wall of the connecting rod is fixedly installed with a mechanical claw, and the lower surface of the robotic arm driving motor is fixedly installed with an injection mechanism.

[0009] As a further description of the above technical solution:

[0010] A magnetic washing box is fixedly installed on the vertical end of the mechanism mounting plate, a detection cup is slidably connected to the inner surface wall of the magnetic washing box, and multiple groups of magnets are fixedly installed inside the magnetic washing box.

[0011] As a further description of the above technical solution:

[0012] An extraction device driving motor is fixedly installed on the outer wall of the first guide rail through a hook, and the output end of the extraction device driving motor is rotatably connected to the extraction device guide rail, and an extraction device slide rail is fixedly installed on the lower surface of the extraction device driving motor, and the other end of the extraction device guide rail is rotatably connected to a rotating wheel, and an extraction mechanism is fixedly installed on the outer wall of the extraction device guide rail through a hook, and the vertical end of the extraction mechanism is slidably connected to the outer wall of the extraction device slide rail.

[0013] As a further description of the above technical solution:

[0014] A spring is fixedly installed on the vertical end of the mechanical claw.

[0015] As a further description of the above technical solution:

[0016] The outer wall of the integrated grabbing and injecting mechanism is slidably connected to the outer wall of the cross rail.

[0017] As a further description of the above technical solution:

[0018] Magnetic beads are movably connected inside the detection cup.

[0019] As a further description of the above technical solution:

[0020] An anti-slip groove is provided on one side of the vertical end of the mechanical claw close to the detection cup.

[0021] As a further description of the above technical solution:

[0022] The outer wall of the rotating wheel is provided with a plurality of anti-skid grooves.

[0023] As a further description of the above technical solution:

[0024] The multiple groups of detection cups are distributed in a matrix inside the incubator.

[0025] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0026] 1. In the present invention, after the sample to be tested is placed in the incubator, the incubator starts to heat up and maintain a constant temperature. The crank starts to rotate, and the connecting rod on the crank makes a circular motion. With the cooperation of the slider, the incubator is driven to oscillate back and forth, and the sample to be tested inside the test cup is shaken while still maintaining a constant temperature. The disadvantage of mutual interference between the incubation and shaking steps is avoided, and the use effect of the chemiluminescence instrument is improved.

[0027] 2. In the present invention, the mechanical claw grabs the test cup and transfers it from the incubator to the magnetic washing box. After washing, it is transported back to the incubation and shaking module from the magnetic washing box. At the same time, the upper injection mechanism can inject clean detergent into the inside of the test cup and put it back into the incubator. The two steps are completed in one process, which improves work efficiency.

[0028] 3. In the present invention, after the test cup is placed in the magnetic washing box, the magnetic beads inside the test cup are attracted by the strong magnet inside the magnetic washing box, and the magnetic beads are adsorbed on the inner wall of the test cup, and a part of the sample to be tested is retained in the gap between the magnetic beads and the inner wall to prevent it from being extracted along with the waste liquid by the extraction device.

[0029] 4. In the present invention, the extraction mechanism is driven by the first guide rail to move to the upper part of the magnetic washing box, and then the extraction device guide rail rotates to drive the extraction mechanism to descend, and extract the washing waste liquid inside the detection cup. Then, the motor reverses to drive the extraction mechanism to reset and continue to extract the next sample. The waste liquid in the sample can be quickly washed clean with the adsorption of magnetic beads, thereby improving the efficiency of extracting waste liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a structural schematic diagram of the present invention;

[0031] Figure 2 It is a schematic diagram of the explosion structure in the present invention;

[0032] Figure 3 This is a structural schematic diagram from another angle in the present invention;

[0033] Figure 4 It is a schematic diagram of the enlarged structure of point A in the present invention.

[0034] Legend:

[0035] 1. Mechanism mounting plate; 2. First guide rail; 3. Incubator; 4. Crank; 5. Slider; 6. Mechanical claw; 7. Connecting rod; 8. Drive rail; 9. Injection mechanism; 10. Magnetic washing box; 11. Detection cup; 12. Extraction device slide rail; 13. Second guide rail; 14. Extraction device guide rail; 15. Extraction mechanism; 16. Cross rail; 17. Incubation and shaking module mounting plate; 18. Rail base. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0037] Example 1:

[0038] See also Figure 1-4 , a chemiluminescence instrument magnetic bead detection pretreatment step integrated mechanism, including a mechanism mounting plate 1, the mechanism mounting plate 1 vertical end fixedly mounted with an incubation and shaking module mounting plate 17, the upper surface of the incubation and shaking module mounting plate 17 is rotatably connected with a crank 4, the lower surface of the incubation and shaking module mounting plate 17 is fixedly mounted with a driving motor, the output end of the driving motor is rotatably connected with a belt, the outer wall of the crank 4 is slidably connected to the outer wall of the belt, the upper surface of the crank 4 is rotatably connected to an incubation box 3, the inner surface wall of the incubation box 3 is slidably connected to multiple groups of detection cups 11, and a slider 5 is fixedly mounted on the lower surface of the incubation box 3, the lower surface of the slider 5 is slidably connected to the upper surface of the incubation and shaking module mounting plate 17, and the multiple groups of detection cups 11 are distributed in a matrix inside the incubation box 3, thereby increasing the number of samples detected.

[0039] After the sample to be tested is placed in the incubator 3, the incubator 3 starts to heat and maintain a constant temperature. The crank 4 starts to rotate, and the connecting rod on the crank 4 makes a circular motion. With the cooperation of the slider 5, the incubator 3 is driven to oscillate back and forth, shaking the sample to be tested inside the test cup 11 while still maintaining a constant temperature, avoiding the disadvantage of the incubation and shaking steps interfering with each other, and improving the use effect of the chemiluminescence instrument.

[0040] The difference from the first embodiment is

[0041] Example 2:

[0042] See also Figure 2 The vertical end of the mechanism mounting plate 1 is fixedly installed with a grabbing and injecting integrated mechanism, and the grabbing and injecting integrated mechanism includes two sets of guide rail seats 18, and the two sets of guide rail seats 18 are respectively connected to the first guide rail 2 and the second guide rail 13 through the transmission wheel. The outer wall of the second guide rail 13 is fixedly installed with a robotic arm driving motor through a hook, and the output end of the robotic arm driving motor is rotatably connected to the driving guide rail 8. The outer wall of the driving guide rail 8 is fixedly installed with a connecting rod 7, and the outer wall of the connecting rod 7 is fixedly installed with a mechanical claw 6. The lower surface of the robotic arm driving motor is fixedly installed with an injection mechanism 9, and the vertical end of the mechanical claw 6 is fixedly installed with a spring, and the vertical end of the mechanism mounting plate 1 is fixedly installed with a cross rail 16. The outer wall of the grabbing and injecting integrated mechanism is slidably connected to the outer wall of the cross rail 16 to improve the stability of the movement. The vertical end of the robotic claw 6 is close to the side of the detection cup 11 with an anti-slip groove to prevent the detection cup 11 from falling.

[0043] The mechanical claw 6 grabs the test cup 11 and transfers it from the incubator 3 to the magnetic washing box 10. After washing, it is transported back to the incubation and shaking module from the magnetic washing box 10. At the same time, the upper injection mechanism 9 can inject clean detergent into the inside of the test cup 11 and put it back into the incubator 3. The two steps are completed in one process, which improves work efficiency.

[0044] The difference from the first embodiment is

[0045] Example 3:

[0046] See also Figure 2 A magnetic washing box 10 is fixedly installed at the vertical end of the mechanism mounting plate 1, and a detection cup 11 is slidably connected to the inner wall of the magnetic washing box 10. Multiple groups of magnets are fixedly installed inside the magnetic washing box 10, and magnetic beads are movably connected inside the detection cup 11 to adsorb the sample to be tested inside the detection cup 11.

[0047] After the test cup 11 is placed in the magnetic washing box 10, the magnetic beads inside the test cup 11 are attracted by the strong magnet inside the magnetic washing box 10, and the magnetic beads are adsorbed on the inner wall of the test cup 11, and part of the sample to be tested is retained in the gap between the magnetic beads and the inner wall to prevent it from being extracted along with the waste liquid by the extraction device.

[0048] The difference from the first embodiment is

[0049] Example 4:

[0050] See also Figure 2 The outer wall of the first guide rail 2 is fixedly installed with an extraction device driving motor through a hook, and the output end of the extraction device driving motor is rotatably connected to the extraction device guide rail 14, and the lower surface of the extraction device driving motor is fixedly installed with an extraction device slide rail 12, and the other end of the extraction device guide rail 14 is rotatably connected to the rotating wheel, and the outer wall of the extraction device guide rail 14 is fixedly installed with an extraction mechanism 15 through a hook, and the vertical end of the extraction mechanism 15 is slidably connected to the outer wall of the extraction device slide rail 12, and the outer wall of the rotating wheel is provided with multiple sets of anti-slip grooves to prevent the extraction device guide rail 14 from slipping.

[0051] Driven by the first guide rail 2, the extraction mechanism 15 moves to the upper part of the magnetic washing box 10, and then the extraction device guide rail 14 rotates, driving the extraction mechanism 15 to descend, and extracting the washing waste liquid inside the detection cup 11. Then, the motor reverses, driving the extraction mechanism 15 to reset, and continue to extract the next sample. With the adsorption of magnetic beads, the waste liquid in the sample can be quickly washed clean, thereby improving the efficiency of extracting waste liquid.

[0052] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A chemiluminescence instrument magnetic bead detection pretreatment step integrated mechanism, comprising a mechanism mounting plate (1), characterized in that: The vertical end of the mechanism mounting plate (1) is fixedly mounted with an incubation and shaking module mounting plate (17), the upper surface of the incubation and shaking module mounting plate (17) is rotatably connected to a crank (4), the lower surface of the incubation and shaking module mounting plate (17) is fixedly mounted with a driving motor, the output end of the driving motor is rotatably connected to a belt, the outer wall of the crank (4) is slidably connected to the outer wall of the belt, the upper surface of the crank (4) is rotatably connected to an incubation box (3), the inner wall of the incubation box (3) is slidably connected to multiple groups of detection cups (11), and a slider (5) is fixedly mounted on the lower surface of the incubation box (3), the lower surface of the slider (5) is slidably connected to the upper surface of the incubation and shaking module mounting plate (17); The vertical end of the mechanism mounting plate (1) is fixedly mounted with a grabbing and injecting integrated mechanism, and the grabbing and injecting integrated mechanism comprises two groups of guide rail seats (18), and the two groups of guide rail seats (18) are respectively rotatably connected to the first guide rail (2) and the second guide rail (13) through transmission wheels, and the outer wall of the second guide rail (13) is fixedly mounted with a manipulator driving motor through a hook, and the output end of the manipulator driving motor is rotatably connected to the driving guide rail (8), and the outer wall of the driving guide rail (8) is fixedly mounted with a connecting rod (7), and the outer wall of the connecting rod (7) is fixedly mounted with a mechanical claw (6), and the lower surface of the manipulator driving motor is fixedly mounted with an injection mechanism (9); A magnetic washing box (10) is fixedly mounted on the vertical end of the mechanism mounting plate (1), a detection cup (11) is slidably connected to the inner surface wall of the magnetic washing box (10), and multiple groups of magnets are fixedly mounted inside the magnetic washing box (10).

2. The integrated mechanism for chemiluminescence magnetic bead detection pretreatment step according to claim 1, characterized in that: An extraction device driving motor is fixedly mounted on the outer wall of the first guide rail (2) via a hook, the output end of the extraction device driving motor is rotatably connected to the extraction device guide rail (14), and an extraction device slide rail (12) is fixedly mounted on the lower surface of the extraction device driving motor, the other end of the extraction device guide rail (14) is rotatably connected to a rotating wheel, an extraction mechanism (15) is fixedly mounted on the outer wall of the extraction device guide rail (14) via a hook, and the vertical end of the extraction mechanism (15) is slidably connected to the outer wall of the extraction device slide rail (12).

3. The integrated mechanism for chemiluminescence magnetic bead detection pretreatment step according to claim 2, characterized in that: A spring is fixedly mounted on the vertical end of the mechanical claw (6).

4. The integrated mechanism for chemiluminescence magnetic bead detection pretreatment step according to claim 3, characterized in that: A transverse rail (16) is fixedly mounted on the vertical end of the mechanism mounting plate (1), and the outer surface wall of the integrated grabbing and injection mechanism is slidably connected to the outer surface wall of the transverse rail (16).

5. The integrated mechanism for chemiluminescence magnetic bead detection pretreatment step according to claim 4, characterized in that: Magnetic beads are movably connected inside the detection cup (11).

6. The integrated mechanism for chemiluminescence magnetic bead detection pretreatment step according to claim 5, characterized in that: An anti-slip groove is provided on one side of the vertical end of the mechanical claw (6) close to the detection cup (11).

7. The integrated mechanism for chemiluminescence magnetic bead detection pretreatment step according to claim 6, characterized in that: The outer wall of the rotating wheel is provided with a plurality of anti-skid grooves.

8. The integrated mechanism for chemiluminescence instrument magnetic bead detection pretreatment step according to claim 1, characterized in that: Multiple groups of the detection cups (11) are distributed in a matrix inside the incubator (3).

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