A magnetic separation device and chemiluminescence immunoassay analyzer

Abstract

The present invention belongs to the technical field of chemical analysis devices, and in particular, relates to a magnetic separation device and a chemiluminescent immunoassay analyzer. The magnetic separation device includes: a sleeve; a slide rod sleeved in the sleeve; a push rod that pushes the slide rod to partially extend out of the sleeve; a locking mechanism located in the sleeve to lock the slide rod when the slide rod partially extends out of the sleeve; an elastic reset member provided between the sleeve and the slide rod; and a magnetic bead adsorbent provided at one end of the slide rod. A slide rod that can be extended and retracted in the sleeve is used. When the push rod pushes the slide rod to partially extend out of the sleeve, the locking mechanism locks the slide rod, and a magnetic bead adsorbent is provided at one end of the slide rod. The magnetic bead adsorbent can contact with a container containing an object to be separated, thereby generating magnetic separation with the object to be separated. The structure is more compact, and the operation process is also simpler and more flexible.

Description

Technical Field

[0001] The invention belongs to the technical field of chemical analysis devices, and in particular relates to a magnetic separation device and a chemiluminescence immunoassay analyzer. Background Art

[0002] In the chemiluminescent immunoassay process, in order to wash away the unbound labeled antibodies / antigens in the immune reaction and obtain the effective luminescent signal carried on the magnetic particle complex, it is necessary to separate the magnetic particle complex from the reaction solution by solid-liquid phase separation using magnetic separation technology.

[0003] In the existing chemiluminescent immunoassay process, the carrier (such as a reaction cup) containing the object to be separated and the magnetic separator are usually independent of each other. When performing magnetic separation, the reaction cup and the magnetic separation device need to be brought close together, and then separated after the magnetic separation is completed, resulting in a relatively complex instrument structure, a large size, and inflexible operation.

[0004] Therefore, designing a magnetic separation device and a chemiluminescent immunoassay analyzer has always been one of the key issues studied by those skilled in the art. Summary of the invention

[0005] The embodiment of the present invention provides a magnetic separation device, aiming to solve the technical problems in the prior art that the magnetic separator and the carrier of the object to be separated are independent of each other, the magnetic separation structure is relatively complex, and the operation is not flexible enough.

[0006] An embodiment of the present invention is implemented as follows: a magnetic separation device includes: a sliding sleeve; a sliding rod sleeved in the sliding sleeve; a push rod that pushes the sliding rod to partially extend out of the sliding sleeve; a locking mechanism located in the sliding sleeve and locking the sliding rod when the sliding rod partially extends out of the sliding sleeve; an elastic reset member provided between the sliding sleeve and the sliding rod; and a magnetic bead adsorption member provided at one end of the sliding rod.

[0007] Furthermore, the locking mechanism includes: a slot provided on the slide rod; a clamping piece provided on the slide sleeve and cooperating with the slot; and a rotation driving component that enables the clamping piece to engage in or disengage from the slot under the push of the push rod.

[0008] Furthermore, a first inclined surface is provided on the clamping member, and the rotation drive assembly includes a first toothed portion provided on the sliding rod and a second toothed portion provided on the push rod, the first toothed portion is provided with a second inclined surface matching the first inclined surface, and the second toothed portion is provided with a third inclined surface matching the second inclined surface.

[0009] Furthermore, the magnetic separation device also includes a boss arranged on the sliding sleeve, a first slide groove arranged on the sliding rod and matching the boss, and a second slide groove arranged on the push rod and matching the boss, and the clamping member is arranged at one end of the boss.

[0010] Furthermore, a placement portion is rotatably provided at one end of the sliding rod, and the magnetic bead adsorption component is arranged on the placement portion.

[0011] Furthermore, the number of the magnetic bead adsorption component is one, and a receiving cavity matching the outer contour of the container in which the magnetic beads are placed is provided in the middle thereof.

[0012] Furthermore, the number of the magnetic bead adsorbing parts is two, and the two magnetic bead adsorbing parts are arranged correspondingly to form a clamping part for clamping the container containing the magnetic beads.

[0013] Furthermore, the magnetic bead adsorption component is a magnet.

[0014] Furthermore, the elastic return member is a tension spring, one end of which is connected to the sliding sleeve, and the other end of which is connected to the placement portion.

[0015] The present invention also provides a chemiluminescent immunoassay analyzer, comprising a host device, a container for placing a reaction solution containing magnetic beads, and the magnetic separation device as described above, wherein the host device is provided with a driving mechanism for driving the push rod to move.

[0016] The beneficial effect achieved by the present invention is that a sliding rod that can be extended and retracted in the sliding sleeve is adopted. When the push rod pushes the sliding rod to partially extend out of the sliding sleeve, the locking mechanism locks the sliding rod, and a magnetic bead adsorption component is provided at one end of the sliding rod. The magnetic bead adsorption component contacts the container containing the object to be separated, thereby producing magnetic separation with the object to be separated; the push rod pushes the sliding rod again, and the sliding rod drives the magnetic bead adsorption component to reset, and the magnetic bead adsorption component is separated from the container. The entire magnetic separation device can be installed integrally with the supporting component, and then installed on the equipment used for chemical analysis. During the magnetic separation process, the two states of adsorption and separation can be realized by pushing the push rod with external force. The structure is more compact and the operation process is simpler and more flexible. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A schematic diagram of a magnetic separation device according to an embodiment of the present invention;

[0018] Figure 2 An exploded schematic diagram of a magnetic separation device according to an embodiment of the present invention;

[0019] Figure 3 A schematic diagram of a magnetic separation device according to an embodiment of the present invention with a sliding rod in a locked state;

[0020] Figure 4 for Figure 3The enlarged view of point A in the middle;

[0021] Figure 5 A schematic diagram of a sliding rod in an initial state according to an embodiment of the present invention;

[0022] Figure 6 A schematic diagram of a slide bar in an ascending state according to an embodiment of the present invention;

[0023] Figure 7 A schematic structural diagram of a magnetic separation device, a container, and a driving mechanism of a chemiluminescent immunoassay analyzer according to an embodiment of the present invention. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0025] See also Figure 1 and Figure 2 , is a preferred embodiment of a magnetic separation device provided by the present invention, the magnetic separation device comprises:

[0026] A sliding sleeve 1, wherein a cavity is provided inside the sliding sleeve 1; a sliding rod 2 is sleeved inside the sliding sleeve 1, wherein the sliding rod 2 is located in the internal cavity of the sliding sleeve 1 and can slide freely axially in the internal cavity of the sliding sleeve 1; a push rod 5 that pushes the sliding rod 2 to partially extend out of the sliding sleeve 1, but the sliding rod 2 will not completely detach from the sliding sleeve 1 under the action of the push rod 5; a locking mechanism located inside the sliding sleeve 1 and locking the sliding rod 2 when the sliding rod 2 partially extends out of the sliding sleeve 1; an elastic reset member 4 provided between the sliding sleeve 1 and the sliding rod 2; and a magnetic bead adsorption member 3 provided at one end of the sliding rod 2.

[0027] By adopting a slide rod 2 that can be extended and retracted in the slide sleeve 1, when the push rod 5 pushes the slide rod 2 to partially extend out of the slide sleeve 1, the locking mechanism locks the slide rod 2. A magnetic bead adsorption component 3 is provided at one end of the slide rod 2. Since the slide rod 2 is in a locked state, the magnetic bead adsorption component 3 is fixed together with a container containing the object to be separated to form a relatively fixed structure. The magnetic bead adsorption component 3 can contact the container containing the object to be separated, thereby generating magnetic separation with the object to be separated. In addition, an elastic reset component 4 is provided between the slide sleeve 1 and the slide rod 2. During the extension of the slide rod 2, the elastic reset component 4 gradually accumulates force. When the slide rod 2 is out of the locked state, the elastic reset component 4 uses the accumulated force to reset the slide rod 2 and retract it to the initial position in the slide sleeve 1.

[0028] See also Figure 2-6 Specifically, the locking mechanism includes:

[0029] A slot 21 is provided on the slide bar 2 and can be a groove; a clamping member 12 is provided on the sleeve 1 and matches the slot 21; and a rotation driving assembly is provided to enable the clamping member 12 to be clamped into or out of the slot 21 under the push of the push rod 5.

[0030] The push rod 5 gradually pushes the slide bar 2 to slide in the sleeve 1. As the slide bar 2 gradually extends out of the sleeve 1, the rotary drive assembly gradually drives the slide bar 2 to rotate axially. When the slide bar 2 rotates and rises to a certain extent, the clamp 12 is inserted into the slot 21. At this time, the push rod 5 no longer gives the slide bar 2 a thrust, and the slide bar 2 has a tendency to reset. However, since the clamp 12 is inserted into the slot 21, the slide bar 2 is blocked from resetting, the slide bar 2 is locked, and the magnetic bead adsorption component 3 interacts with the object to be separated. Subsequently, the push rod 5 continues to give the slide bar 2 a thrust, and the rotary drive assembly drives the slide bar 2 to rotate again. The clamp 12 is disengaged from the slot 21, and under the action of the elastic reset member 4, the slide bar 2 is reset.

[0031] Specifically, refer to Figure 2-6 , the clamping member 12 is provided with a first inclined surface 121, the rotation driving assembly includes a first toothed portion 22 provided on the slide bar 2 and a second toothed portion 51 provided on the push rod 5, the clamping groove 21 can be a groove between adjacent first toothed portions 22, the first toothed portion 22 is provided with a second inclined surface 221 matching the first inclined surface 121, and the second toothed portion 51 is provided with a third inclined surface 511 matching the second inclined surface 221. The matching referred to above can be that the first inclined surface 121 and the second inclined surface 221 have the same inclination angle, and the two act on each other, and can be that the second inclined surface 221 and the third inclined surface 511 have the same inclination angle, and the two act on each other.

[0032] The push rod 5 pushes the slide bar 2 to rise. When the slide bar 2 extends out of the sleeve 1 to a certain extent, the push rod 5 stops giving thrust to the slide bar 2. The third inclined surface 511 is tangent to the second inclined surface 221. The second inclined surface 221 will slide down along the third inclined surface 511 with the reset trend, rotate axially and enter above the first inclined surface 121. At this time, the clamp 12 enters the clamping groove 21 to achieve the locking of the slide bar 2.

[0033] The push rod 5 pushes the slide bar 2 up again, and when the slide bar 2 gradually extends out of the sleeve 1, the second inclined surface 221 follows the rise of the first inclined surface 121. The second inclined surface 221 leaves the first inclined surface 121, that is, the clamping member 12 is separated from the clamping slot 21, and the elastic reset member 4 is elastically reset, driving the slide bar 2 to axially rotate and reset, and quickly retract to the initial position inside the sleeve 1.

[0034] Further, see Figure 2 and Figure 4The magnetic separation device also includes a boss 11 provided on the sleeve 1, a first chute 23 provided on the slide bar 2 and matching with the boss 11, and a second chute 52 provided on the push rod 5 and matching with the boss 11. The matching mentioned above may be that the shape of the boss 11 is consistent with the shape of the cavity on the first chute 23, or that the shape of the boss 11 is consistent with the shape of the cavity on the second chute 52. The clamp 12 is provided at one end of the boss 11, and the clamp 12 and the boss 11 are integrally provided. The boss 11 is provided on the inner wall of the sleeve 1 and extends along the axis direction of the sleeve 1, the first chute 23 is provided on the slide bar 2 and extends along the axis direction of the slide bar 2, and the second chute 52 is provided on the push rod 5 and extends along the axis direction of the push rod 5.

[0035] When the push rod 5 does not push the slide bar 2, the slide bar 2 is at the initial position, and the boss 11 is located in the first slide groove 23 and the second slide groove 52. As the slide bar 2 rises, the boss 11 slides on the first slide groove 23 and the second slide groove 52, and the first slide groove 23 and the second slide groove 52 guide the boss 11. After the second inclined surface 221 enters the first inclined surface 121, the boss 11 is separated from the first slide groove 23 and the second slide groove 52, and the clamping member 12 at one end of the boss 11 smoothly enters the clamping groove 21. After the second inclined surface 221 leaves the first inclined surface 121, the boss 11 enters the first slide groove 23 and the second slide groove 52, and the slide bar 2 is reset.

[0036] Optionally, three bosses 11 are evenly arranged inside the sliding sleeve 1, and the adjacent bosses 11 are spaced 120 degrees apart; three first slide grooves 23 are evenly arranged on the sliding rod 2, and the adjacent first slide grooves 23 are spaced 120 degrees apart; six first toothed portions 22 are arranged on the sliding rod 2, and one of the adjacent first toothed portions 22 is connected to the first slide groove 23; three second slide grooves 52 are evenly arranged on the push rod 5, and the adjacent second slide grooves 52 are spaced 120 degrees apart; six second toothed portions 51 are arranged on the push rod 5, and one of the adjacent second toothed portions 51 is connected to the second slide groove 52. In this way, it can be ensured that during the rotation of the sliding rod 2, the clamping member 12 can be inserted into or separated from the clamping slot 21, and when the sliding rod 2 rotates and rises for the first time, the clamping member 12 will be inserted into the clamping slot 21, and when the sliding rod 2 rotates and rises for the second time, the clamping member 12 will be separated from the clamping slot 21.

[0037] Of course, the number of bosses 11, first slide grooves 23, and second slide grooves 52 is not limited to the above, but the numbers of the three are consistent with each other. The first tooth-shaped portions 22 and the second tooth-shaped portions 51 are also not limited to the above, but the numbers of the two are consistent with each other. Moreover, the number of bosses 11 is half of the number of first tooth-shaped portions 22.

[0038] Further, see Figure 2One end of the slide bar 2 is rotatably provided with a placement portion 6, and the magnetic bead adsorption member 3 is arranged on the placement portion 6. The placement portion 6 rotatably connected to the slide bar 2 is provided, so that when the slide bar 2 is pushed and rotated by the push rod 5 during the extension process, the magnetic bead adsorption member 3 arranged on the placement portion 6 remains stationary. In this embodiment, the magnetic adsorption member is a magnet.

[0039] Specifically, see Figure 2 The slide bar 2 reset member is a tension spring, one end of which is connected to the sliding sleeve 1 and the other end is connected to the placement portion 6. The slide bar 2 reset member is connected to the placement portion 6, so that the slide bar reset member 4 is not twisted by the slide bar 2 when the slide bar 2 rotates.

[0040] In this embodiment, the number of the magnetic bead adsorbent 3 can be one, and a receiving cavity matching the outer contour of the container 7 containing the magnetic beads is provided in the middle thereof. This ensures that the magnetic bead adsorbent 3 can be in more comprehensive contact with the container 7, and better separate the magnetic beads in the container 7 from the reaction solution.

[0041] In addition, see Figure 2 , the magnetic bead adsorbing part 3 can also be two pieces, and the two magnetic bead adsorbing parts 3 are arranged correspondingly to form a clamping part for clamping the container 7 containing the magnetic beads. In this embodiment, the placement part 6 can be made of two spring sheets or elastic plastic materials, so that the magnet can be in close contact with the outer wall of the container 7, and can better adsorb the magnetic beads in the container 7, so that the magnetic beads and the reaction liquid can be better separated. Among them, the two placement parts 6 can be separated or integrally arranged on the sliding rod 2 to form a clamping part for clamping the reaction cup, and the magnetic bead adsorbing part 3 is arranged on the side where the two placement parts 6 contact the container 7.

[0042] See also Figure 7 The present invention also provides a chemiluminescent immunoassay analyzer, including a host device, a container 7 for placing a magnetic bead reaction solution, and the above-mentioned magnetic separation device. The host device is provided with a driving mechanism 8 for driving the slide bar 2 to partially extend out of the slide sleeve 1. Specifically, the driving mechanism 8 drives the push rod 5 to move, and the push rod 5 pushes the slide bar 2 to partially extend out of the slide sleeve 1. By using the driving mechanism 8 to drive the slide bar 2 of the magnetic separation device to extend and retract, automated production is achieved.

[0043] In this embodiment, the driving mechanism 8 can be a motor or a cylinder.

[0044] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A magnetic separation device, characterized in that: include: Sliding sleeve; A sliding rod sleeved in the sliding sleeve; A push rod that pushes the slide rod portion out of the slide sleeve; A locking mechanism is located in the sliding sleeve and locks the sliding rod when the sliding rod partially extends out of the sliding sleeve, and a placement portion is rotatably provided at one end of the sliding rod; an elastic return member disposed between the sliding sleeve and the sliding rod; and A magnetic bead adsorption component provided at one end of the sliding rod, the magnetic bead adsorption component is provided at the placement portion, and the magnetic bead adsorption component is a magnet; The number of the magnetic bead adsorbent is one, and the center thereof is provided with a receiving cavity matching the outer contour of the container containing the magnetic beads, or the number of the magnetic bead adsorbent is two, and the two magnetic bead adsorbents are arranged correspondingly to form a clamping portion for clamping the container containing the magnetic beads.

2. The magnetic separation device according to claim 1, characterized in that: The locking mechanism comprises: A slot provided on the slide bar; A clamping member provided on the sliding sleeve and matched with the clamping slot; A rotation drive assembly is provided to enable the card member to be inserted into or separated from the card slot under the push of the push rod.

3. The magnetic separation device according to claim 2, characterized in that: The clamp is provided with a first inclined surface, and the rotation drive assembly includes a first toothed portion provided on the slide rod and a second toothed portion provided on the push rod, the first toothed portion is provided with a second inclined surface matching the first inclined surface, and the second toothed portion is provided with a third inclined surface matching the second inclined surface.

4. The magnetic separation device according to claim 3, characterized in that: The magnetic separation device also includes a boss arranged on the sliding sleeve, a first slide groove arranged on the sliding rod and matching with the boss, and a second slide groove arranged on the push rod and matching with the boss, and the clamping member is arranged at one end of the boss.

5. The magnetic separation device according to claim 1, characterized in that: The elastic return member is a tension spring, one end of which is connected to the sliding sleeve, and the other end of which is connected to the placement portion.

6. A chemiluminescent immunoassay analyzer, characterized in that: It comprises a host device, a container for placing a reaction solution containing magnetic beads, and the magnetic separation device as described in any one of claims 1 to 5, wherein the host device is provided with a driving mechanism for driving the push rod to move.

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