A parathyroid hormone secretion monitoring and regulation system

Abstract

An embodiment of the present invention discloses a parathyroid hormone secretion monitoring and regulation system, comprising: a calcium ion measurement subsystem for real-time measuring the concentration value of calcium ions in a solution to be measured and uploading the concentration value to an upper computer subsystem; a light regulation subsystem for receiving a control instruction from the upper computer subsystem to achieve regulation of light flux, so that the regulated light irradiates the solution to be measured; and an upper computer subsystem for configuring the calcium ion measurement subsystem and generating a control instruction according to the calcium ion concentration value uploaded by the calcium ion measurement subsystem, and sending the control instruction to the light regulation subsystem to achieve parathyroid PTH secretion monitoring and regulation. The embodiment of the present invention makes up for the technical blank in the prior art of improving the calcium ion sensing ability of patient chief cells and regulating PTH secretion; it can realize real-time monitoring of the calcium ion concentration in a solution to be measured, and regulate PTH secretion according to the calcium ion concentration to achieve the metabolic balance of PTH.

Description

Technical Field

[0001] The embodiments of the present invention relate to the field of optoelectronic technologies, and particularly to a parathyroid hormone secretion monitoring and regulation system. Background Art

[0002] Parathyroid hormone (PTH) is an important calcium and phosphorus homeostasis regulator secreted by the chief cells of the parathyroid gland. It plays an important role in maintaining the balance of blood calcium and phosphorus and bone metabolism in the body, and can regulate its own expression and secretion according to changes in blood calcium concentration. In patients with hyperparathyroidism, the serum PTH level is chronically higher than that of normal people. According to the mechanism of calcium ion reverse regulation of PTH secretion, it indicates that the mechanism of chief cells in hyperparathyroidism patients to sense calcium ions is disordered. If we can help the chief cells of hyperparathyroidism patients regain the calcium response ability, then we can help the chief cells restore the normal PTH secretion function and even cure hyperparathyroidism.

[0003] At present, the main clinical treatments for hyperparathyroidism include parathyroidectomy and drug therapy. Parathyroidectomy combined with forearm parathyroid transplantation can quickly reduce the body's PTH level. However, due to the important role of PTH in regulating the body's calcium and phosphorus balance, symptoms of calcium and phosphorus disorders often occur after surgery. Commonly used drugs for the clinical treatment of hyperparathyroidism include active vitamin D, calcitriol, and calcium mimetics. The drug treatment effect is not significant and is often accompanied by various side effects. Among them, the calcium mimetic has a better treatment effect, but it is ineffective for about 30% of patients and often causes side effects such as gastrointestinal symptoms.

[0004] So far, there has been no report on a system for regulating PTH secretion by improving the calcium ion sensing ability of patients' chief cells. Therefore, developing a system that can combine online calcium ion measurement and PTH secretion regulation function has broad market value and application prospects. Summary of the Invention

[0005] The embodiments of the present invention provide a parathyroid hormone secretion monitoring and regulation system to combine the calcium ion measurement function with the PTH secretion regulation function, improve the automation level of calcium ion measurement, and help chief cells regain the ability to accurately sense external calcium ion changes and regulate their own PTH secretion.

[0006] The embodiments of the present invention provide a parathyroid hormone secretion monitoring and regulation system, which includes:

[0007] A calcium ion measurement subsystem, a light regulation subsystem, and an upper computer subsystem;

[0008] Among them, the calcium ion measurement subsystem is used to measure the concentration value of calcium ions in the solution to be measured in real time and upload the concentration value to the upper computer subsystem;

[0009] The light regulation subsystem is used to receive the control instruction of the upper computer subsystem, realize the regulation of the light flux, and make the regulated light irradiate the solution to be measured;

[0010] The upper computer subsystem is used to configure the calcium ion measurement subsystem, generate the control instruction according to the calcium ion concentration value uploaded by the calcium ion measurement subsystem, and send the control instruction to the light regulation subsystem to realize the monitoring and regulation of parathyroid hormone secretion.

[0011] Optionally, the calcium ion measurement subsystem includes:

[0012] A calcium ion measurement electrode for detecting the calcium ion concentration in the solution to be measured and converting the calcium ion concentration into an electrical signal;

[0013] A signal amplifier for amplifying the electrical signal by a preset multiple;

[0014] A signal conversion circuit for converting the amplified electrical signal into a digital signal;

[0015] A numerical calculation module for converting the digital signal into a calcium ion concentration value;

[0016] A communication interface for establishing a connection with the upper computer subsystem to realize data interaction.

[0017] Optionally, the numerical calculation module is a single-chip microcomputer.

[0018] Optionally, the light regulation subsystem includes:

[0019] A communication interface for establishing a connection with the upper computer subsystem to receive the control instruction;

[0020] A light source and a light source drive circuit for making the light source work according to the control instruction.

[0021] Optionally, the light source is an LED lamp panel.

[0022] Optionally, the upper computer subsystem generates the control instruction according to the calcium ion concentration value uploaded by the calcium ion measurement subsystem, including:

[0023] When the calcium ion concentration value is higher than the first threshold, determine the light illumination strategy according to the difference between the calcium ion concentration value and the first threshold, and generate the control instruction with the light illumination strategy, where the light illumination strategy includes at least one parameter of light intensity, driving light source delay time, and light illumination frequency;

[0024] When the calcium ion concentration value is lower than the second threshold, a control instruction is generated to turn off the light source, where the second threshold is less than or equal to the first threshold.

[0025] Optionally, the configuration of the calcium ion measurement subsystem by the host subsystem includes setting the sampling frequency of the calcium ion measurement subsystem and / or calibrating the calcium ion measurement subsystem.

[0026] Optionally, the system further includes a display module for displaying the calcium ion concentration value and the light source operating parameters.

[0027] Optionally, the host subsystem communicates with the calcium ion measurement subsystem and the light regulation subsystem respectively through a wireless communication module.

[0028] Optionally, a vector for regulating parathyroid hormone secretion is expressed in the solution to be measured.

[0029] In the embodiment of the present invention, a parathyroid PTH secretion monitoring and regulation system is obtained by combining a calcium ion measurement subsystem and a light regulation subsystem, so that the host subsystem controls the working state of the light regulation subsystem according to the calcium ion concentration of the solution to be measured, thereby detecting and regulating the secretion of parathyroid PTH, filling the technical gap in the prior art of improving the calcium ion sensing ability of patient chief cells and regulating PTH secretion; it can realize real-time monitoring of the calcium ion concentration in the solution to be measured, and regulate PTH secretion according to the calcium ion concentration to achieve the metabolic balance of PTH. Description of the Drawings

[0030] Figure 1 is a schematic structural diagram of the parathyroid hormone secretion monitoring and regulation system in the embodiment of the present invention;

[0031] Figure 2 is a schematic structural diagram of the calcium ion measurement subsystem in the embodiment of the present invention;

[0032] Figure 3 is a schematic structural diagram of the light regulation subsystem in the embodiment of the present invention;

[0033] Figure 4 is a schematic functional module structure diagram of the host subsystem in the embodiment of the present invention.

[0034] Figure 5 is the parathyroid hormone secretion monitoring and regulation system with the light source turned off in the embodiment of the present invention;

[0035] Figure 6 is the parathyroid hormone secretion monitoring and regulation system with the light source turned on in the embodiment of the present invention. Detailed Embodiments

[0036] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It can be understood that the specific embodiments described herein are only used to explain the present invention, rather than limiting the present invention. In addition, it should be noted that, for the sake of convenience of description, only the parts related to the present invention rather than all the structures are shown in the drawings.

[0037] Embodiment

[0038] Figure 1 FIG. is a schematic structural diagram of a parathyroid hormone secretion monitoring and regulation system provided by an embodiment of the present invention. This embodiment is applicable to the situation of monitoring the calcium ion concentration in a solution to be measured and regulating the light regulation system according to the calcium ion concentration to adjust the PTH concentration in the solution to be measured.

[0039] As Figure 1 shown, the structure of the parathyroid hormone secretion monitoring and regulation system specifically includes:

[0040] A calcium ion measurement subsystem, a light regulation subsystem, and an upper computer subsystem.

[0041] Among them, the calcium ion measurement subsystem is used to measure the concentration value of calcium ions in the solution to be measured in real time and upload the concentration value to the upper computer subsystem. Specifically, the calcium ion measurement subsystem includes: a calcium ion measurement electrode for detecting the calcium ion concentration in the solution to be measured and converting the calcium ion concentration into an electrical signal; a signal amplifier for amplifying the electrical signal by a preset multiple; a signal conversion circuit for converting the amplified electrical signal into a digital signal; a numerical calculation module for converting the digital signal into a calcium ion concentration value. The numerical calculation module can use a single-chip microcomputer to convert the calcium ion concentration value; a communication interface for establishing a connection with the upper computer subsystem to realize data interaction. In this embodiment, the type of the communication interface is not limited, and a relatively conventional RS485 communication interface can be selected. Further, the calcium ion measurement subsystem can also configure working parameters (such as sampling frequency) or complete calibration and initialization and other work under the control of the upper computer subsystem.

[0042] The reason for choosing the calcium ion concentration as the measurement target parameter of the test solution is determined according to the characteristics of the test solution. The test solution is usually the body fluid of a human or an animal, or a specially prepared test solution, which contains PTH and expresses a carrier that regulates the secretion of PTH by the parathyroid gland. PTH is an important calcium and phosphorus homeostasis regulator secreted by the chief cells of the parathyroid gland and plays an important role in maintaining the balance of blood calcium and phosphorus and bone metabolism in the body. The single chain of the PTH protein consists of 84 amino acids and can regulate its own expression and secretion according to the change of blood calcium concentration. Under normal circumstances, CaSR (Calcium Sensing Receptor, CaSR) on the chief cells of the parathyroid gland senses the change of calcium ions in the body environment; when the calcium ion concentration in the blood increases, a large number of calcium ions bind to CaSR on the surface of the chief cells, which can inhibit the cAMP (Cyclic Adenosine monophosphate) pathway and reduce the synthesis of PTH; on the other hand, after calcium ions bind to CaSR, it can activate the PLC (Phospholipases C), MAPK (mitogen-activated protein kinases) and PLA2 (Phospholipases A2) pathways and inhibit the release of PTH; jointly achieving the purpose of reducing blood calcium. Conversely, when the blood calcium concentration decreases, CaSR is not bound by enough calcium ions, thus activating the cAMP pathway and inhibiting the PLC, MAPK and PLA2 pathways to promote the synthesis and release of PTH; promoting osteoclastogenesis and accelerating the increase of blood calcium.

[0043] The PTH level in the serum of patients with hyperparathyroidism is higher than that of normal people for a long time. According to the mechanism of calcium ion reverse regulation of PTH secretion, it shows that the mechanism of chief cells sensing calcium ions in patients with hyperparathyroidism is disordered; some studies have shown that the CaSR expressed by the chief cells of patients with hyperparathyroidism is significantly lower than that of the normal control; suggesting that insufficient CaSR expression may be the main reason for the excessive secretion of PTH by chief cells. That is, there is not enough CaSR to accurately sense the change of blood calcium and then guide the PTH secretion of chief cells; thus triggering excessive secretion of PTH and then leading to the occurrence of hyperparathyroidism. If we can help the chief cells of patients with hyperparathyroidism regain the calcium response ability, then we can help the chief cells restore the normal PTH secretion function and even cure hyperparathyroidism. Then, a calcium ion measuring electrode can sensitively sense the change of calcium ion concentration. The calcium ion measuring electrode is an electrochemical sensor, and its structure has a sensitive membrane that has a linear response to calcium ions, which converts the ion activity into a potential signal. Within a certain range, its potential has a linear relationship with the logarithm of the activity of specific ions in the solution.

[0044] Further, the light regulation subsystem is used to receive control instructions from the upper-level subsystem, regulate the light flux, and make the regulated light irradiate the solution to be measured. Specifically, the light regulation subsystem includes: a communication interface for establishing a connection with the upper-level subsystem and receiving control instructions; a light source and a light source drive circuit for making the light source work according to the control instructions. Specifically, the light source is an LED lamp panel, and the wavelength of the LED is selected from the blue light band, and the wavelength of the blue light is 450-500 nm, for example, it can be 450 nm, 460 nm, 470 nm, 480 nm, 490 nm or 500 nm, preferably 460-480 nm. This is because there is a carrier that regulates the secretion of parathyroid PTH. This carrier includes a photosensitive gene and / or a PTH promoter that specifically recognizes parathyroid chief cells. The photosensitive gene is a gene that can express a cell membrane channel protein sensitive to blue light. The photosensitive protein can open the channel under irradiation at a specific wavelength (such as 470 nm), allowing cations (sodium ions, calcium ions, etc.) to enter the cytoplasm, resulting in an increase in the cell membrane potential, thereby realizing the regulation of cell activity. Then, through optogenetic regulation technology, using a specific promoter, the PTH promoter that can specifically recognize parathyroid chief cells can be used to recognize parathyroid chief cells, and the PTH secretion of parathyroid chief cells can be regulated by irradiating with blue light of a specific wavelength and frequency, so as to achieve the purpose of highly spatiotemporally precisely and specifically regulating parathyroid chief cells.

[0045] The control instructions issued by the upper-level subsystem may include parameters such as the flashing frequency of the light source, the start time and duration of the light source. For example, the delay time can be set, and the light source is turned on to irradiate the solution to be measured after the preset delay time ends. The light source can also work in a working mode of irradiating for 1 second and turning off for 1 second. Preferably, the frequency of the blue light is 15-25 Hz, for example, it can be 15 Hz, 16 Hz, 17 Hz, 18 Hz, 19 Hz, 20 Hz, 21 Hz, 22 Hz, 23 Hz, 24 Hz or 25 Hz, preferably 18-22 Hz. Preferably, the average light intensity of the blue light is 8-12 mW / mm2, for example, it can be 8 mW / mm2, 9 mW / mm.

[0046] Further, the upper computer subsystem is used to configure the calcium ion measurement subsystem, generate the control instruction according to the calcium ion concentration value uploaded by the calcium ion measurement subsystem, and send the control instruction to the light regulation subsystem to realize the monitoring and regulation of parathyroid PTH secretion. Specifically, the upper computer subsystem generates a control instruction according to the calcium ion concentration value uploaded by the calcium ion measurement subsystem, including: when the calcium ion concentration value is higher than the first threshold, determining the light illumination strategy according to the difference between the calcium ion concentration value and the first threshold, and generating the control instruction with the light illumination strategy, wherein the light illumination strategy includes at least one parameter of light intensity, driving light source delay time, and light illumination frequency; for example, the larger the difference, the higher the calcium ion concentration, and the stronger the intensity and / or duration of the light illumination are required. When the calcium ion concentration value is lower than the second threshold, a control instruction is generated to turn off the light source, wherein the second threshold is less than or equal to the first threshold. The first threshold is an upper limit threshold, and the second threshold is a lower limit threshold.

[0047] The upper computer subsystem can also configure the calcium ion measurement subsystem, including setting the sampling frequency of the calcium ion measurement subsystem and / or calibrating the calcium ion measurement subsystem.

[0048] Preferably, the parathyroid hormone secretion monitoring and regulation system further includes: a display module for displaying the calcium ion concentration value and the light source working parameters.

[0049] Preferably, the upper computer subsystem communicates with the calcium ion measurement subsystem and the light regulation subsystem respectively through a wireless communication module.

[0050] In one implementation, reference can be made to Figure 5 and Figure 6 the parathyroid hormone secretion monitoring and regulation system shown, wherein, Figure 5 when the calcium ion concentration is lower than the lower limit threshold (such as lower than 50 mg / L), it is the system when the blue light source is not turned on, and at this time, PTH secretion is not inhibited; Figure 6 when the calcium ion concentration is higher than the upper limit threshold (such as higher than 50 mg / L), it is the system when the blue light source is turned on, and at this time, PTH secretion is inhibited. When the calcium ion measurement subsystem detects in real time that the calcium ion concentration drops, the working parameters of the blue light source, such as light intensity, can also be adjusted until the calcium ion concentration drops below 50 mg / L, and the blue light source is turned off to stop inhibiting PTH secretion. In Figure 5 and Figure 6 only the experimental brackets in the laboratory environment are shown, which are used to fix or place the calcium ion detection electrode and the solution to be measured, as well as the display module.

[0051] The technical solution of this embodiment obtains a parathyroid hormone secretion monitoring and regulation system by combining a calcium ion measurement subsystem and a light regulation subsystem, enabling the upper computer subsystem to control the working state of the light regulation subsystem in real time according to the calcium ion concentration of the solution to be measured, thereby detecting and regulating the secretion of parathyroid PTH, filling the technical gap in improving the calcium ion sensing ability of patient chief cells and regulating PTH secretion in the prior art; it can realize real-time monitoring of the calcium ion concentration in the solution to be measured, regulate PTH secretion according to the calcium ion concentration, and achieve the metabolic balance of PTH.

[0052] Note that the above is only the preferred embodiment of the present invention and the technical principles applied. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein. Various obvious changes, re-adjustments, and substitutions can be made by those skilled in the art without departing from the protection scope of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments. Without departing from the concept of the present invention, more other equivalent embodiments can be included, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. A parathyroid hormone secretion monitoring and regulation system, characterized in that, Comprising: a calcium ion measurement subsystem, a light regulation subsystem, and a host computer subsystem; wherein, the calcium ion measurement subsystem is used to measure the concentration value of calcium ions in the solution to be measured in real time and upload the concentration value to the host computer subsystem; wherein, there is a carrier for regulating the secretion of parathyroid hormone in the solution to be measured, and the carrier includes a photosensitive gene and a PTH promoter that specifically recognizes parathyroid chief cells; the light regulation subsystem is used to receive the control instruction of the host computer subsystem, realize the regulation of the light flux, and make the regulated light irradiate the solution to be measured; wherein, the control instruction includes the flashing frequency of the light source, the start time and duration of the light source, the illumination frequency, and the light intensity; the host computer subsystem is used to configure the calcium ion measurement subsystem, generate the control instruction according to the calcium ion concentration value uploaded by the calcium ion measurement subsystem, and send the control instruction to the light regulation subsystem to realize the monitoring and regulation of parathyroid hormone secretion; wherein, the host computer subsystem generates the control instruction according to the calcium ion concentration value uploaded by the calcium ion measurement subsystem, including: when the calcium ion concentration value is higher than the first threshold, determine the illumination strategy according to the difference between the calcium ion concentration value and the first threshold, and generate the control instruction with the illumination strategy, wherein the illumination strategy includes at least one parameter of light intensity, driving light source delay time, and illumination frequency; when the calcium ion concentration value is lower than the second threshold, generate the control instruction to turn off the light source, wherein the second threshold is less than or equal to the first threshold; wherein, the light regulation subsystem includes: a communication interface, used to establish a connection with the host computer subsystem and receive the control instruction; a light source and a light source drive circuit, used to make the light source work according to the control instruction.

2. The system according to claim 1, characterized in that, The calcium ion measurement subsystem includes: a calcium ion measurement electrode, used to detect the calcium ion concentration in the solution to be measured and convert the calcium ion concentration into an electrical signal; a signal amplifier, used to amplify the electrical signal by a preset multiple; a signal conversion circuit, used to convert the amplified electrical signal into a digital signal; a numerical calculation module, used to convert the digital signal into a calcium ion concentration value; a communication interface, used to establish a connection with the host computer subsystem to realize data interaction.

3. The system according to claim 2, characterized in that, The numerical calculation module is a single-chip microcomputer.

4. The system according to claim 1, characterized in that, The light source is an LED lamp panel.

5. The system according to any one of claims 1-4, characterized in that, The host computer subsystem configuring the calcium ion measurement subsystem includes setting the sampling frequency of the calcium ion measurement subsystem and / or calibrating the calcium ion measurement subsystem.

6. The system according to any one of claims 1-4, characterized in that, The system further includes: a display module, used to display the calcium ion concentration value and the light source working parameters.

7. The system according to any one of claims 1-4, characterized in that, The host computer subsystem communicates with the calcium ion measurement subsystem and the light regulation subsystem respectively through a wireless communication module.

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