Traditional Chinese medicine extract composition for treating intestinal cancer, preparation method and application of traditional Chinese medicine extract composition in preparation of drugs

Abstract

The invention relates to a traditional Chinese medicine extract composition for treating an intestinal cancer, a preparation method and application of the traditional Chinese medicine extract composition in preparation of drugs. Raw pharmaceutical material extracts serve as various ingredients, wherein a weight ratio of a radix sophorae flavescentis extract to a gentian root extract is (1 to 15):(1 to 15). The radix sophorae flavescentis extract is an aqueous extract; and the gentian root extract is an aqueous extract. Distinguished from the traditional viewpoint that traditional Chinese medicine compounded co-decoction is superior to separate extracting of all medicinal materials, the invention proposes an optimal combination method for all medicinal material extracts, accurate control on the composition is achieved, pharmacological effects are improved remarkably, and the method has a positive guiding action on treatment methods for the intestinal cancer by the traditional Chinese medicine extract composition.

Description

Technical field

[0001] The present invention relates to a composition of a Chinese medicine extract for the treatment of intestinal cancer, a method of preparation of a composition for intestinal cancer; and a composition for the treatment of intestinal cancer in the preparation of intestinal cancer drugs. Background technique

[0002] Intestinal cancer refers to cancer that occurs in the intestine, mainly colon cancer and rectal cancer, one of the most common malignant tumors worldwide. Due to the change of people's living environment and habits, the incidence of intestinal cancer in my country has increased year by year. Intestinal cancer can spread to other tissues and organs by lymph, blood circulation and direct spread, early non-obvious symptoms, etc. When discovery, patients often missed the time of surgery. Due to the complex mechanism of colorectal cancer, more induced factors, chemicals are often difficult to achieve satisfactory therapeutic effects, while these drugs h...

Examples

Embodiment 1

[0020] Example 1 Preparation of Traditional Chinese Medicine Extract

[0021] 1, drug feedstock

[0022] Squirting, gallbladder.

[0023] 2, preparation method for extractance

[0024] Squirting extract: the bitter ginseng medicinal material is pulverized into 12-tit-coated powder, soaking for 1 hour, water extraction 3 times, extracting temperature 80 ~ 90 ° C, 1 hour, 10 times water, and the stirring speed is 20 rpm during extraction. Combined extract, filtration, 3000 r / min centrifuge for 10 minutes, take the supernatant, concentrated to 1 g of halogen, spare;

[0025] Dragon extract: the long gallbladder material is pulverized into 12th glue powder, soaking for 1 hour, water extraction 3 times, extracting temperature 80 ~ 90 ° C, 1 hour, 10 times water, and the stirring speed is 20 rpm during extraction. Combined extract, filtration, 3000 r / min centrifuged for 10 minutes, take the supernatant, concentrated to 1 g of halody, spare.

Embodiment 2

[0026] Example 2 Extract Combination Pharmaceutical on the synergistic effect of inhibiting intestinal cancer cells

[0027] 1, experimental materials

[0028] (1) Drugs: bitter ginseng extract, galloyer extract.

[0029] (2) Intestinal cancer cells: human intestinal cancer cells HCT116 cells, HT29 cells.

[0030] (3) MTT kit.

[0031] 2, experiment group

[0032] (1) The concentration gradient of the bitter ginseng extract.

[0033] (2) The galloyer extract concentration gradient.

[0034] (3) Comprehensive combination of bitter ginsets and galloyer extracts.

[0035] 3, MTT method to detect the sensitivity of tumor cells to different Chinese medicine extracts

[0036] (1) HCT116 cells in human intestinal cancer cells, HT29 cells are inoculated with 5000 to 9000 cells per well to 96-well plates, and after the cells are attached, the bitter ginseng extracts of different pharmaceutical concentrations are added, and the gallbladder extracts are added. Or combined PBS solutions. Afte...

Embodiment 3

[0038] Example 3 Effect of Different Combined Components of Traditional Chinese Medicine Extract on Tumor Cell Inhibition

[0039] 1, experimental materials

[0040] (1) Drugs: bitter ginseng extract, galloyer extract.

[0041] (2) Intestinal cancer cells: human intestinal cancer cells HCT116 cells, HT29 cells.

[0042] (3) MTT kit.

[0043] 2, experiment group

[0044] Phase of pseudo-extracts and galloyer extracts.

[0045] 3, MTT method to detect the sensitivity of tumor cells to different Chinese medicine extracts

[0046] (1) HCT116 cells in human intestinal cancer cells, HT29 cells are inoculated with 5,000 to 9000 cells per well to 96-well plates, and after the cells are attached to the wall, they are added to the piratory substances and gallbladder extracts of different pharmaceutical concentrations, respectively. Combined PBS solution. After 24 h, 10 μl of the concentration of 5 mg / ml of MTT was added to a MTT recharge for 4 h, and then 100 μl of DMSO was added per well...

Technical field

[0001] The present invention relates to a composition of a Chinese medicine extract for the treatment of intestinal cancer, a method of preparation of a composition for intestinal cancer; and a composition for the treatment of intestinal cancer in the preparation of intestinal cancer drugs. Background technique

[0002] Intestinal cancer refers to cancer that occurs in the intestine, mainly colon cancer and rectal cancer, one of the most common malignant tumors worldwide. Due to the change of people's living environment and habits, the incidence of intestinal cancer in my country has increased year by year. Intestinal cancer can spread to other tissues and organs by lymph, blood circulation and direct spread, early non-obvious symptoms, etc. When discovery, patients often missed the time of surgery. Due to the complex mechanism of colorectal cancer, more induced factors, chemicals are often difficult to achieve satisfactory therapeutic effects, while these drugs h...

Examples

Embodiment 1

[0020] Example 1 Preparation of Traditional Chinese Medicine Extract

[0021] 1, drug feedstock

[0022] Squirting, gallbladder.

[0023] 2, preparation method for extractance

[0024] Squirting extract: the bitter ginseng medicinal material is pulverized into 12-tit-coated powder, soaking for 1 hour, water extraction 3 times, extracting temperature 80 ~ 90 ° C, 1 hour, 10 times water, and the stirring speed is 20 rpm during extraction. Combined extract, filtration, 3000 r / min centrifuge for 10 minutes, take the supernatant, concentrated to 1 g of halogen, spare;

[0025] Dragon extract: the long gallbladder material is pulverized into 12th glue powder, soaking for 1 hour, water extraction 3 times, extracting temperature 80 ~ 90 ° C, 1 hour, 10 times water, and the stirring speed is 20 rpm during extraction. Combined extract, filtration, 3000 r / min centrifuged for 10 minutes, take the supernatant, concentrated to 1 g of halody, spare.

Embodiment 2

[0026] Example 2 Extract Combination Pharmaceutical on the synergistic effect of inhibiting intestinal cancer cells

[0027] 1, experimental materials

[0028] (1) Drugs: bitter ginseng extract, galloyer extract.

[0029] (2) Intestinal cancer cells: human intestinal cancer cells HCT116 cells, HT29 cells.

[0030] (3) MTT kit.

[0031] 2, experiment group

[0032] (1) The concentration gradient of the bitter ginseng extract.

[0033] (2) The galloyer extract concentration gradient.

[0034] (3) Comprehensive combination of bitter ginsets and galloyer extracts.

[0035] 3, MTT method to detect the sensitivity of tumor cells to different Chinese medicine extracts

[0036] (1) HCT116 cells in human intestinal cancer cells, HT29 cells are inoculated with 5000 to 9000 cells per well to 96-well plates, and after the cells are attached, the bitter ginseng extracts of different pharmaceutical concentrations are added, and the gallbladder extracts are added. Or combined PBS solutions. Afte...

Embodiment 3

[0038] Example 3 Effect of Different Combined Components of Traditional Chinese Medicine Extract on Tumor Cell Inhibition

[0039] 1, experimental materials

[0040] (1) Drugs: bitter ginseng extract, galloyer extract.

[0041] (2) Intestinal cancer cells: human intestinal cancer cells HCT116 cells, HT29 cells.

[0042] (3) MTT kit.

[0043] 2, experiment group

[0044] Phase of pseudo-extracts and galloyer extracts.

[0045] 3, MTT method to detect the sensitivity of tumor cells to different Chinese medicine extracts

[0046] (1) HCT116 cells in human intestinal cancer cells, HT29 cells are inoculated with 5,000 to 9000 cells per well to 96-well plates, and after the cells are attached to the wall, they are added to the piratory substances and gallbladder extracts of different pharmaceutical concentrations, respectively. Combined PBS solution. After 24 h, 10 μl of the concentration of 5 mg / ml of MTT was added to a MTT recharge for 4 h, and then 100 μl of DMSO was added per well...

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