Microcurrent nanoneedle wafer device and method of introducing exosomes

Abstract

The present application relates to instrument technology, and solves the problem that the effect is not obvious when directly using a nanometer microneedle chip to introduce exosomes, and provides a micro-current nanometer microneedle chip device and a method for introducing exosomes, and the technical scheme can be summarized as follows: the micro-current nanometer microneedle chip device comprises a nanometer microneedle chip, a first electrode, a second electrode and a power input module, the nanometer microneedle chip has a through hole in the center, the first electrode is fixed in the through hole and is adapted to the shape of the through hole, the second electrode is a ring electrode and is fixed around the periphery of the nanometer microneedle chip, the power input module supplies power to the nanometer microneedle chip and provides a first charge to the first electrode and a second charge to the second electrode, and in use, the nanometer microneedle chip, the first electrode and the second electrode can all be in contact with the skin to be treated. The present application has the beneficial effect that the absorption of exosomes by the skin is greatly increased when introducing the exosomes, and is suitable for nanometer microneedle chips.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of instruments, in particular to the technical field of nanometer microneedle wafer. BACKGROUND

[0002] The nanometer microneedle wafer is a nanometer penetration special tool developed according to the structure of human skin, which is carved from single crystal silicon through nanometer technology process. The surface of the nanometer wafer is a series of microneedle arrays, the microneedles can penetrate the stratum corneum and open the channel to the dermis, so that the required drugs can more easily penetrate into the dermal cells.

[0003] Under the stimulation of a certain intensity of electric pulse, the cell will produce a short-term electroporation effect, so that the semi-permeability of the cell membrane is temporarily lost, and the absorption of the cell to the drug and DNA macromolecules is increased. In recent years, it has been applied in gene transfection, cell fusion, and improvement of drug cancer treatment effect. Compared with traditional drug treatment, the treatment combined with electroporation can improve the drug absorption rate to a certain extent.

[0004] Exosomes are nanoscale vesicles with a diameter of 30-200nm secreted by cells, have a phospholipid bilayer structure, participate in signal transmission between cells, and various physiological and pathological processes of organisms. Due to the special structure and function of exosomes, it has potential application value, on the one hand, it can be used as a biological indicator for diagnosing various diseases; on the other hand, it can also be used as a drug carrier as a treatment method; in addition to this, exosomes are also applied to the medical and beauty industry for skin care.

[0005] When exosomes are used for skin care, the absorption rate of direct application on the skin surface is very low, less than 2%, and it is difficult to be used as a normal means. The current medical community's tools for promoting cosmetic absorption include invasive microneedles, ultrasound and electric fields, physical microwaves, lasers and chemical penetration enhancers. But these methods either penetrate the skin too deeply to form a painful wound and easily cause infection; or have irritation to the skin and damage the health of the skin.

[0006] If the nanometer microneedle wafer is directly used to introduce exosomes, its effect is not as good as the existing technology such as electroporation, so it is necessary to improve the nanometer microneedle wafer to improve its use effect. SUMMARY

[0007] The purpose of the present application is to solve the problem that the effect is not obvious when the nanometer microneedle wafer is directly used to introduce exosomes, and to provide a micro-current nanometer microneedle wafer device and a method for introducing exosomes.

[0008] The application solves the above technical problems, and the technical scheme is that the micro-current nanometer microneedle wafer device comprises a nanometer microneedle wafer, a first electrode, a second electrode and a power input module, the nanometer microneedle wafer has a through hole in the center, the first electrode is fixed in the through hole and has a shape suitable for the through hole, the second electrode is a ring electrode and is fixed around the periphery of the nanometer microneedle wafer, the power input module provides a first charge to the first electrode and a second charge to the second electrode, if the first charge is a positive charge, the second charge is a negative charge, and vice versa, if the first charge is a negative charge, the second charge is a positive charge, and in use, the nanometer microneedle wafer, the first electrode and the second electrode can all be in contact with the skin to be treated.

[0009] Specifically, to provide a shape of the through hole, the through hole is circular or regular polygonal.

[0010] Further, to provide a ring electrode, the outer edge of the ring electrode is circular or regular polygonal, and the inner edge is suitable for the outer edge of the nanometer microneedle wafer.

[0011] Specifically, to provide a parameter range of the nanometer microneedle wafer, the periphery of the nanometer microneedle wafer is rectangular, the length and width are both 3-10 mm, the number of array protrusions is 64-100, the height of the array protrusions is 50-300 μm, and the taper is (40±3°).

[0012] Further, to provide a parameter range of the use of the micro-current nanometer microneedle wafer device, the working frequency of the direct current pulse current between the first electrode and the second electrode is 5-30 HZ, and the output power is 2.5-15 W.

[0013] Specifically, to further improve the effect of the micro-current nanometer microneedle wafer device in use, a vibration generator is further included, the side of the nanometer microneedle wafer with array protrusions is the front side, the back side is fixed on the vibration generator, and the vibration generator can make the nanometer microneedle wafer vibrate in a direction perpendicular to the front side of the nanometer microneedle wafer in use.

[0014] Further, to avoid damage to the nanometer microneedle wafer caused by direct contact between the vibration generator and the nanometer microneedle wafer, a gasket is further included, the gasket is located between the vibration generator and the nanometer microneedle wafer, and the back side of the nanometer microneedle wafer being fixed on the vibration generator means that the nanometer microneedle wafer is fixed on the gasket, and the gasket is fixed on the vibration generator.

[0015] Specifically, to provide a working parameter of the vibration generator, the working frequency of the vibration generator is 5-20 HZ, and the power is 10-30 W.

[0016] The method for introducing exosomes is only used for introducing exosomes for skin care, comprising the following steps:

[0017] The exosome mixture is applied to the skin, and before using the micro-current nanometer microneedle wafer device, the working parameters of the current between the first electrode and the second electrode in the micro-current nanometer microneedle wafer device are set, and the micro-current nanometer microneedle wafer device is used to introduce the exosome mixture applied to the skin into the skin.

[0018] Specifically, when the micro-current nanometer microneedle wafer device also has a vibration generator, in order to improve the effect of the micro-current nanometer microneedle wafer device in use, when the micro-current nanometer microneedle wafer device is used to introduce the exosome mixture applied to the skin into the skin, the working parameters of the vibration generator are also set to a frequency of 5-20 HZ and a power of 10-30 W, and the vibration generator is allowed to work.

[0019] Further, in order to propose a concentration range of the exosome mixture and a use parameter range of the micro-current nanometer microneedle wafer device, the concentration of the exosome mixture is 200 μg / ml; the working frequency of the current between the first electrode and the second electrode is 5-30 HZ, and the output power is 2.5-15 W.

[0020] Specifically, in order to explain in detail how to use the micro-current nanometer microneedle wafer device to introduce the exosome mixture applied to the skin into the skin, the micro-current nanometer microneedle wafer device is placed on the skin applied with the exosome mixture, and the array of the nanometer microneedle wafer, the first electrode and the second electrode in the micro-current nanometer microneedle wafer device are in contact with the skin, and work continuously for at least 5 seconds in the same contact area.

[0021] The beneficial effect of the present application is that in the present application, the micro-current nanometer microneedle wafer device combines electroporation technology and nanometer wafer technology, which can improve the effect of the nanometer microneedle wafer in use, ensure safety, stimulate cells with micro-current and nanometer microneedle wafer, open the channel of the skin, and greatly increase the absorption of the skin to the exosome, so that the exosome can act on the dermis layer, and the damage to the skin is small, the healing is fast, and the skin is not easy to be damaged, which can better repair the skin and reduce the pain trauma. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 is a structure diagram of the micro-current nanometer microneedle wafer device in the embodiment of the present application.

[0023] Figure 2 is a sectional view of the micro-current nanometer microneedle wafer device in the embodiment of the present application.

[0024] Wherein, 1 is the first electrode, 2 is the nanometer microneedle wafer, 3 is the second electrode, 4 is the gasket, and 5 is the vibration generator. DETAILED DESCRIPTION

[0025] The technical solutions of the present application will be described in detail below with reference to the embodiments and drawings.

[0026] The structure diagram of the micro-current nanometer microneedle wafer device of the present application is shown in the figure, which comprises a nanometer microneedle wafer 2, a first electrode 1, a second electrode 3, and a power input module. Figure 1 The nanometer microneedle wafer 2 has a through hole in the center, the first electrode 1 is fixed in the through hole and its shape is adapted to the through hole, the second electrode 3 is a ring electrode and is fixed around the periphery of the nanometer microneedle wafer 2, the power input module provides a first charge to the first electrode 1 and a second charge to the second electrode 3, if the first charge is a positive charge, the second charge is a negative charge, and vice versa, if the first charge is a negative charge, the second charge is a positive charge, and in use, the nanometer microneedle wafer 2, the first electrode 1 and the second electrode 3 can all be in contact with the skin to be treated.

[0027] Since the first electrode 1 has a positive charge and the second electrode 3 has a negative charge, when the micro-current nanometer microneedle wafer device is in contact with the skin, the first electrode 1 and the second electrode 3 are also in contact with the skin, and the positive charge will flow from the first electrode 1 to the second electrode 3 through the skin, during which process the positive charge can open the cell membrane of the cells and promote the absorption of nutrients, achieving the purpose of combining electroporation technology and nanometer wafer technology.

[0028] To provide a through hole, the through hole is preferably circular or regular polygonal, and the through hole can be of various shapes, preferably circular or regular polygonal, which can better utilize the material, reduce material waste, and facilitate use.

[0029] To provide a ring electrode, the outer edge of the ring electrode is preferably circular or regular polygonal, and the inner edge is adapted to the outer edge of the nanometer microneedle wafer, and the outer edge of the ring electrode can also be of various shapes, preferably circular or regular polygonal, which can better utilize the material, reduce material waste, and facilitate use.

[0030] To provide a parameter range of the nanometer microneedle wafer 2, it is preferably that the periphery of the nanometer microneedle wafer 2 is rectangular, the length and width are both 3-10 mm, the number of array protrusions is 64-100, the height of the array protrusions is 50-300 pm, and the taper is (40±3°).

[0031] To provide a parameter range for use of the micro-current nanometer microneedle wafer device, the working frequency of the direct current pulse current between the first electrode 1 and the second electrode 3 is preferably 5-30 HZ, and the output power is preferably 2.5-15 W.

[0032] In order to further improve the effect of the micro-current nanometer microneedle wafer device in use, a vibration generator 5 can also be included, and the side of the nanometer microneedle wafer 2 with the array of protrusions is the front side, and the back side is fixed on the vibration generator 5, and the vibration generator 5 can make the nanometer microneedle wafer vibrate in a direction perpendicular to the front side of the nanometer microneedle wafer when in use.

[0033] In order to avoid damage to the nanometer microneedle wafer 2 caused by direct contact between the vibration generator 5 and the nanometer microneedle wafer 2, a gasket 4 can also be included, which is located between the vibration generator 5 and the nanometer microneedle wafer 2, and the back side of the nanometer microneedle wafer 2 is fixed on the vibration generator 5, which means that the nanometer microneedle wafer 2 is fixed on the gasket 4, and the gasket is fixed on the vibration generator 5.

[0034] In order to provide a range of working parameters of the vibration generator 5, the working frequency of the vibration generator 5 is preferably 5-20HZ, and the power is preferably 10-30W.

[0035] The method for introducing exosomes according to the present application comprises the following steps:

[0036] The exosome mixture is applied to the skin, and before using the micro-current nanometer microneedle wafer device, the working parameters of the current between the first electrode and the second electrode in the micro-current nanometer microneedle wafer device are set, and the exosome mixture applied to the skin is introduced into the skin using the micro-current nanometer microneedle wafer device.

[0037] When the micro-current nanometer microneedle wafer device also has a vibration generator 5, in order to improve the effect of the micro-current nanometer microneedle wafer device in use, the working parameters of the vibration generator 5 are set to a frequency of 5-20HZ and a power of 10-30W when the exosome mixture applied to the skin is introduced into the skin using the micro-current nanometer microneedle wafer device, and the vibration generator 5 is allowed to work.

[0038] In order to propose a range of concentrations of the exosome mixture and the use parameters of the micro-current nanometer microneedle wafer device, the concentration of the exosome mixture is preferably 200ug / ml, the working frequency of the current between the first electrode 1 and the second electrode 3 is preferably 5-30HZ, and the output power is preferably 2.5-15W.

[0039] To explain in detail how to use the micro-current nanometer microneedle wafer device to introduce the exosome mixture smeared on the skin into the skin, only for introducing exosomes for skin care, the micro-current nanometer microneedle wafer device is preferably used to introduce the exosome mixture smeared on the skin into the skin: the micro-current nanometer microneedle wafer device is placed on the skin smeared with the exosome mixture, and the array of the nanometer microneedle wafer, the first electrode and the second electrode in the micro-current nanometer microneedle wafer device are in contact with the skin, and the same contact area is continuously worked for at least 5 seconds. The principle is: when the first electrode and the second electrode are in contact with the skin, an electric pulse current is generated between the first electrode and the second electrode through the skin, thereby a transient electroporation effect is generated in the skin cells, at the same time, the microneedle can penetrate the stratum corneum and open a channel to the dermis, the two are combined, so that the absorption of the skin to the exosome is greatly increased.

[0040] Embodiment

[0041] To compare and explain the basis for selecting various parameters, specific experiments are as follows:

[0042] I. In vitro transdermal effect comparison experiment:

[0043] 1. Healthy SD rats were sacrificed by cervical dislocation, the abdominal skin and hair were removed, 2 cm x 2 cm skin was cut, the subcutaneous tissue and fat were carefully peeled off with forceps, rinsed in physiological saline for 30 min, the excess water was absorbed with filter paper, wrapped with aluminum foil, and stored at -20°C for standby, and thawed naturally before experiment.

[0044] 2. The treated rat skin of each group was fixed in a horizontal diffusion cell, with the stratum corneum facing the supply chamber and the dermis facing the receiving chamber. The assembled horizontal diffusion cell was placed on a constant temperature magnetic stirrer, 10 ml of 0.9% physiological saline was added to the receiving chamber, the temperature of the two chambers was 37(±0.5)℃, and there should be no bubbles between the skin and the physiological saline liquid surface. The device was divided into blank control group (A group), micro-current nanometer microneedle wafer device experiment group (B group) and perforation experiment group (C group). 0.5 ml of exosome solution with a concentration of 200 μg / ml was smeared on the rat skin in group A, and 0.5 ml of exosome solution with a concentration of 200 μg / ml was smeared on the rat skin in group B, and then the micro-current nanometer microneedle wafer device (current frequency 10HZ, output power 5W, without starting the vibration generator) was used to treat the rat skin, the nanometer microneedle in the micro-current nanometer microneedle wafer device was perpendicular to the skin surface, and the first electrode and the second electrode in the micro-current nanometer microneedle wafer device were in contact with the skin, and the treatment was continued for at least 5 seconds, then removed. In group C, 0.5 ml of exosome solution with a concentration of 200 μg / ml was injected with a 4 number syringe, the needle was inclined at about 20 degrees, and the rat skin dermis was gently penetrated. Among them, the exosome solution is mesenchymal stem cell exosome solution.

[0045] 3. The horizontal diffusion cell was stirred evenly by a constant temperature magnetic stirrer. 2 ml of the receiving solution was taken at 10 s, 30 s, 1 min, 2 min and 3 min, respectively. After each sampling, 2 ml of standby receiving solution was immediately added. The concentration of exosomes in each group of receiving solution was determined, and the average permeation rate was calculated. The results were expressed as x ± s, and one-way ANOVA was performed using SPSS 22.0 statistical software. P < 0.05 was considered to be significantly different.

[0046] 4. The experimental results showed that the permeation rate of exosomes after treatment by the micro-current nanometer microneedle chip device and after puncture injection treatment was significantly higher than that of the blank control group without treatment. There was no significant difference in the permeation rate between the micro-current nanometer microneedle chip device treatment and the puncture injection treatment, as shown in Table 1.

[0047] Table 1 Average permeation rate of each group

[0048] Packet Average transmittance (x 10 -3 μg / cm 2 ·s)]]> A 1.76±0.21 B 8.47±0.26 C 8.53±0.18

[0049] It can be seen that the micro-current nanometer microneedle chip device or puncture injection can effectively improve the introduction efficiency of exosomes.

[0050] II. Comparison of skin wound effects

[0051] 1. After the completion of Experiment 1, the mouse skin after transdermal penetration for 3 min was taken out, fixed with 10% formaldehyde solution, and embedded with paraffin. The deparaffinized sections were stained with hematoxylin staining solution for 10 min, washed with water, placed in 2% hydrochloric acid alcohol for 30 s, continued to wash with tap water for 15 minutes, stained with 1% eosin for 2 min, repeatedly washed with tap water, and finally dehydrated with low concentration gradient alcohol, dried in a drying machine at 37°C, and finally obtained neutral gum mounting. The skin structure was observed under a microscope with different magnifications.

[0052] 2. The experimental results showed that the mouse skin structure in group A had no obvious changes, the dermis layer in group C had large needle holes, and the subcutaneous tissue damage was more serious, and the dermis layer in group B had small needle holes, and the subcutaneous tissue damage was smaller. The experimental results showed that the micro-current nanometer microneedle chip had less damage to the skin under the condition that the permeation rate of the micro-current nanometer microneedle chip treatment and the puncture injection treatment had no significant difference. It can be seen that the micro-current nanometer microneedle chip device method has less damage to the skin.

[0053] III. Comparison of transdermal effects under different intensity currents

[0054] 1. The treated rat skin was divided into five groups and placed in a horizontal diffusion cell with the stratum corneum facing the supply chamber and the dermis facing the receiving chamber. The assembled horizontal diffusion cell was placed on a constant temperature magnetic stirrer. The receiving chamber was added with 10 ml of 0.9% physiological saline, the temperature of the two chambers was 37(±0.5)℃, there should be no bubbles between the liquid surface and the skin, and the stirrer was stirred at a constant speed. The mouse skin was smeared with 0.5 ml of exosome solution with a concentration of 200 μg / ml, the parameters of the microcurrent nanometer microneedle chip device were set as follows: frequency 10 Hz, output power 0 W, 2.5 W, 5 W, 10 W and 15 W, respectively, and the vibration generator was not turned on. The mouse skin was treated, and the nanometer microneedle in the microcurrent nanometer microneedle chip device was perpendicular to the skin surface, and the first electrode and the second electrode in the microcurrent nanometer microneedle chip device were in contact with the skin. Each part of the skin was vertically and continuously treated for 5 s and then removed. Among them, the exosome solution was mesenchymal stem cell exosome solution.

[0055] 2. The constant temperature magnetic stirrer stirred the horizontal diffusion cell at a constant speed, and 2 ml of receiving liquid was taken at 10 s, 30 s, 1 min, 2 min and 3 min, respectively. After each sampling, 2 ml of standby receiving liquid was immediately added, and the exosome concentration in each group of receiving liquid was determined, and the average permeability was calculated. The results were expressed as x±s, and single factor analysis of variance was performed using SPSS22.0 statistical software, and P<0.05 was considered to be significantly different.

[0056] 3. The experimental results showed that when the output power was 5 W, the absorption effect of exosomes was the best, but when the output power was 2.5 W to 15 W, the absorption effect of exosomes was significantly improved compared with 0 W, as shown in Table 2.

[0057] Table 2 Average permeability under different output power

[0058] Output power Average transmittance (x 10 -3 μg / cm 2 ·s)]]> 0W 5.16±0.31 2.5W 6.83±0.17 5W 8.54±0.21 10W 8.23±0.34 15W 7.14±0.27

[0059] Four, comparison experiment of transdermal exosomes with different concentrations

[0060] 1. The treated rat skin was divided into five groups and placed in a horizontal diffusion cell with the stratum corneum facing the supply chamber and the dermis facing the receiving chamber. The assembled horizontal diffusion cell was placed on a constant temperature magnetic stirrer. The receiving chamber was added with 10 ml of 0.9% physiological saline, the temperature of the two chambers was 37(±0.5)℃, there should be no bubbles between the liquid surface and the skin, and the stirrer was stirred at a constant speed. On the five groups of rat skin, 0.5 ml of exosome solution with concentration gradient of 50 μg / ml, 100 μg / ml, 200 μg / ml, 300 μg / ml and 400 μg / ml was applied respectively, and the micro-current nanometer microneedle chip device (frequency 10HZ, output power 5W, vibration generator not turned on) was used to treat the rat skin. The nanometer microneedle in the micro-current nanometer microneedle chip device was perpendicular to the skin surface, and the first electrode and the second electrode in the micro-current nanometer microneedle chip device were in contact with the skin. After 5s of vertical continuous action on each part of the skin, it was removed. Among them, the exosome solution was mesenchymal stem cell exosome solution.

[0061] 2. The constant temperature magnetic stirrer stirred the horizontal diffusion cell at a constant speed, and 2 ml of receiving liquid was taken at 10s, 30s, 1 min, 2 min and 3 min respectively. After each sampling, 2 ml of standby receiving liquid was immediately added, and the exosome concentration in each group of receiving liquid was determined to calculate the average permeability. The results were expressed as x±s, and single factor analysis of variance was performed by SPSS22.0 statistical software, P<0.05 was significantly different.

[0062] 3. The experimental results showed that the higher the exosome concentration, the higher the skin absorption efficiency, but when the exosome concentration exceeded 200 μg / ml, the skin absorption efficiency did not increase significantly, and it had basically reached saturation state. As shown in Table 3.

[0063] Table 3 Average permeability when using different concentrations of exosome mixture

[0064] Exosome concentration (pg / ml) Average transmittance (x 10 -3 μg / cm 2 ·s)]]> 50 2.13±0.24 100 4.56±0.36 200 8.52±0.42 300 8.72±0.26 400 8.80±0.31

[0065] Five, comparative experiment of using different frequency vibration generator

[0066] 1. The treated mouse skin in each group was placed in a horizontal diffusion cell with the stratum corneum facing the supply chamber and the dermis facing the receiving chamber. The assembled horizontal diffusion cell was placed on a constant temperature magnetic stirrer, and 10 ml of 0.9% physiological saline was added to the receiving chamber. The temperature of the two chambers was 37(±0.5)℃, and there should be no bubbles between the skin and the liquid surface. 0.5 ml of exosome solution with a concentration of 200 μg / ml was applied to the mouse skin in five groups, and a micro-current nanometer microneedle chip device (frequency 10 Hz, output power 5 W) was used to treat the mouse skin. The vibration generator had frequencies of 0 Hz, 5 Hz, 10 Hz, and 15 Hz, respectively. The nanometer microneedle in the micro-current nanometer microneedle chip device was perpendicular to the skin surface, and the first and second electrodes in the micro-current nanometer microneedle chip device were in contact with the skin. Each part of the skin was treated vertically for 5 s, and then removed. The exosome solution was a mesenchymal stem cell exosome solution.

[0067] 2. The constant temperature magnetic stirrer stirred the horizontal diffusion cell at a constant speed. 2 ml of receiving liquid was taken at 10 s, 30 s, 1 min, 2 min, and 3 min, respectively. After each sampling, 2 ml of standby receiving liquid was added immediately. The exosome concentration in each group of receiving liquid was determined, and the average permeability was calculated. The results were expressed as x±s, and one-way ANOVA was used for statistical analysis using SPSS 22.0 statistical software. P<0.05 was considered statistically significant.

[0068] 3. The experimental results showed that when the vibration generator frequency was 10 Hz, the average permeability was in the best state, and when the frequency exceeded 10 Hz, the average permeability did not increase significantly, as shown in Table 5. At the same time, if the frequency of the vibration generator was too high, it might damage the cell epidermis.

[0069] Table 5 Average permeability with or without vibration generator

[0070] Vibration generator generation frequency Average transmittance (x 10 -3 μg / cm 2 ·s)]]> 0 HZ 8.50±0.37 5 HZ 8.68±0.25 10 HZ 9.36±0.13 15 HZ 9.41±0.24

Claims

1. A micro-current nanoneedle chip device, comprising a nanoneedle chip, a first electrode, a second electrode and a power input module, the nanoneedle chip has a through hole in the center, the first electrode is fixed in the through hole and its shape is adapted to the through hole, the second electrode is a ring electrode and is fixed around the periphery of the nanoneedle chip, the power input module provides a first charge to the first electrode and a second charge to the second electrode, if the first charge is a positive charge, the second charge is a negative charge, and vice versa, in use, the nanoneedle chip, the first electrode and the second electrode can all contact the skin to be treated. The periphery of the nanoneedle chip is rectangular, with a length and width of 3-10 mm, the number of array protrusions is 64-100, the height of the array protrusions is 50-300 μm, and the taper is (40±3°). The working frequency of the direct current pulse current between the first electrode and the second electrode is 5-30 Hz, and the output power is 2.5-15 W.

2. The microcurrent nanoneedle wafer device of claim 1, wherein, The through hole is circular or a regular polygon.

3. The microcurrent nanoneedle wafer device of claim 2, wherein, The outer edge of the ring electrode is circular or a regular polygon, and the inner edge is adapted to the outer edge of the nanoneedle chip.

4. The microcurrent nanoneedle wafer device of any one of claims 1-3, wherein, It also includes a vibration generator, the side of the nanoneedle chip with array protrusions is the front side, and the back side is fixed on the vibration generator, the vibration generator can make the nanoneedle chip vibrate in a direction perpendicular to the front side of the nanoneedle chip when in use.

5. The microcurrent nanoneedle wafer device of claim 4, wherein, It also includes a gasket between the vibration generator and the nanoneedle chip, and the back side of the nanoneedle chip is fixed on the vibration generator, which means that the nanoneedle chip is fixed on the gasket, and the gasket is fixed on the vibration generator.

6. The microcurrent nanoneedle wafer device of claim 4, wherein, The working frequency of the vibration generator is 5-20 Hz, and the power is 10-30 W.

7. A method of introducing an exosome, characterized by, It is only used for introducing exosomes for skin care, comprising the following steps: The exosome mixture is applied to the skin, and the working parameters of the current between the first electrode and the second electrode in the micro-current nanoneedle chip device as claimed in any one of claims 1-6 are set before using the micro-current nanoneedle chip device, and the exosome mixture applied to the skin is introduced into the skin by using the micro-current nanoneedle chip device.

8. The method of introducing exosomes of claim 7, wherein, The concentration of the exosome mixture is 200 μg / ml, the working frequency of the current between the first electrode and the second electrode is 5-30 Hz, and the output power is 2.5-15 W.

9. The method of introducing exosomes according to any one of claims 7-8, wherein, The exosome mixture applied to the skin is introduced into the skin by using the micro-current nanoneedle chip device, which means that the micro-current nanoneedle chip device is placed on the skin with the exosome mixture, and the array protrusions of the nanoneedle chip in the micro-current nanoneedle chip device, the first electrode and the second electrode contact the skin, and work continuously for at least 5 seconds in the same contact area.

Images