Method for improving phellinus igniarius cultivation efficiency through laboratory intelligent cultivation management

Abstract

The invention discloses a method for improving phellinus igniarius cultivation efficiency through laboratory intelligent cultivation management. The method comprises the steps of quality improvement and optimization of a phellinus igniarius mother strain solid plate culture medium, intermittent propagation culture of a phellinus igniarius liquid strain, optimization of the culture medium through a mulberry twig bag material, intelligent spawn running, fruiting cultivation management and the like. According to the method, the culture period of mycelium and sporophore of phellinus igniarius is short, the biotransformation efficiency is high, and the content of bioactive components such as polysaccharide and triterpene is high. According to the method, laboratory intelligent management is remotely controlled through a mobile phone, cultivation conditions are remotely, flexibly and accurately controlled in an all-weather mode, and the hypha bag filling time is shortened to about 40 days. After the fungus bags are opened, the cultivation period of the sporophore of phellinus igniarius is shortened to about 70 days, compared with a cultivation method in the prior art, the cultivation period is shortened by 60 days or above, and the method achieves high biotransformation efficiency and sporophore yield.

Description

technical field

[0001] The invention relates to the technical field of medicinal and edible fungus cultivation, in particular to a method for improving the cultivation efficiency of Phellinus phellidis by intelligent cultivation management in a laboratory. Background technique

[0002] Phellinus mulberry is a precious Chinese herbal medicine growing on mulberry trees. It has been used as a traditional Chinese medicine for more than 2,000 years. "Describes Phellinus as "long-term service, making light of life and prolonging life", so Phellinus has the reputation of "forest gold". At present, many institutions in China have preserved Phellinus species and have carried out a series of research. Phellinus anticancer effect was first announced by the Japanese National Cancer Research Center in 1968. The experiment showed that the water extract of the wild Phellinus fruiting body had an inhibitory effect on mouse sarcoma S180 tumor cells, and the proliferation inhibition rate was...

Examples

Embodiment 2

[0048] Optimization of the quality improvement of the solid plate medium of the Phellinus radix parent species: under sterile conditions, after inoculating the activated Phellinus slant parent species on the solid plate medium of the Phellinus chinensis species, cultured at 26°C for 10 days to optimize the quality. Fast growing, dense and vigorous, solid Phellinus strains with neat edges for use.

[0049] The components used in the solid plate medium are: 230g / L potato, 23g / L glucose, 0.3g / L lignin, 1.2g / LMgSO 4 , 1.5g / LKH 2 PO 4 , 1.5ml / L wood vinegar, 18.0g / L agar, the remainder water, and adjust the pH to 6.0.

[0050] Phellinus liquid strain intermittent expansion culture: liquid expansion medium at a temperature of 121 ° C, autoclaved for 30 minutes, and cooled in a sterile environment. Under aseptic conditions, 10 pieces of Phellinus fungi with a diameter of 0.6cm round cakes were placed in the liquid medium and cultured with intermittent shaking. Under sterile dark c...

Embodiment 3

[0057] Optimization of the quality improvement of the solid plate medium of the Phellinus radix parent species: under sterile conditions, after inoculating the activated Phellinus slant parent species on the solid plate medium of the Phellinus chinensis species, cultured at 26°C for 10 days to optimize the quality. Fast growing, dense and vigorous, solid Phellinus strains with neat edges for use.

[0058] The ingredients used in the solid plate medium are: 200g / L potato, 24g / L glucose, 0.2g / L lignin, 1.1g / LMgSO 4 , 1.4g / LKH 2 PO 4 , 2.0ml / L wood vinegar, 17.0g / L agar, the balance of water, and adjust the pH to 6.0.

[0059] Phellinus liquid strain intermittent expansion culture: liquid expansion medium at a temperature of 121 ° C, autoclaved for 30 minutes, and cooled in a sterile environment. Under aseptic conditions, 10 pieces of Phellinus fungi with a diameter of 0.6cm round cakes were placed in the liquid medium and cultured with intermittent shaking. Under sterile dark...

Embodiment 4

[0067] Optimization of the quality improvement of the solid plate medium of Phellinus chinensis: Under sterile conditions, after inoculating the activated Phellinus slant mother species on the solid plate medium of Phellinus chinensis, cultured at 25°C for 10 days to improve the quality, select Fast growing, dense and vigorous, solid Phellinus strains with neat edges for use.

[0068] The ingredients used in the solid plate medium are: 220g / L potato, 20g / L glucose, 0.4g / L lignin, 1.3g / LMgSO 4 , 1.6g / LKH 2 PO 4 , 3.0ml / L wood vinegar, 16.0g / L agar, the balance of water, and adjust the pH to 6.0.

[0069] Phellinus liquid strain intermittent expansion culture: liquid expansion medium at a temperature of 121 ° C, autoclaved for 30 minutes, and cooled in a sterile environment. Under aseptic conditions, 10 pieces of Phellinus fungi with a diameter of 0.6cm round cakes were placed in the liquid medium and cultured with intermittent shaking. Under sterile dark conditions, the temp...

technical field

[0001] The invention relates to the technical field of medicinal and edible fungus cultivation, in particular to a method for improving the cultivation efficiency of Phellinus phellidis by intelligent cultivation management in a laboratory. Background technique

[0002] Phellinus mulberry is a precious Chinese herbal medicine growing on mulberry trees. It has been used as a traditional Chinese medicine for more than 2,000 years. "Describes Phellinus as "long-term service, making light of life and prolonging life", so Phellinus has the reputation of "forest gold". At present, many institutions in China have preserved Phellinus species and have carried out a series of research. Phellinus anticancer effect was first announced by the Japanese National Cancer Research Center in 1968. The experiment showed that the water extract of the wild Phellinus fruiting body had an inhibitory effect on mouse sarcoma S180 tumor cells, and the proliferation inhibition rate was...

Examples

Embodiment 2

[0048] Optimization of the quality improvement of the solid plate medium of the Phellinus radix parent species: under sterile conditions, after inoculating the activated Phellinus slant parent species on the solid plate medium of the Phellinus chinensis species, cultured at 26°C for 10 days to optimize the quality. Fast growing, dense and vigorous, solid Phellinus strains with neat edges for use.

[0049] The components used in the solid plate medium are: 230g / L potato, 23g / L glucose, 0.3g / L lignin, 1.2g / LMgSO 4 , 1.5g / LKH 2 PO 4 , 1.5ml / L wood vinegar, 18.0g / L agar, the remainder water, and adjust the pH to 6.0.

[0050] Phellinus liquid strain intermittent expansion culture: liquid expansion medium at a temperature of 121 ° C, autoclaved for 30 minutes, and cooled in a sterile environment. Under aseptic conditions, 10 pieces of Phellinus fungi with a diameter of 0.6cm round cakes were placed in the liquid medium and cultured with intermittent shaking. Under sterile dark c...

Embodiment 3

[0057] Optimization of the quality improvement of the solid plate medium of the Phellinus radix parent species: under sterile conditions, after inoculating the activated Phellinus slant parent species on the solid plate medium of the Phellinus chinensis species, cultured at 26°C for 10 days to optimize the quality. Fast growing, dense and vigorous, solid Phellinus strains with neat edges for use.

[0058] The ingredients used in the solid plate medium are: 200g / L potato, 24g / L glucose, 0.2g / L lignin, 1.1g / LMgSO 4 , 1.4g / LKH 2 PO 4 , 2.0ml / L wood vinegar, 17.0g / L agar, the balance of water, and adjust the pH to 6.0.

[0059] Phellinus liquid strain intermittent expansion culture: liquid expansion medium at a temperature of 121 ° C, autoclaved for 30 minutes, and cooled in a sterile environment. Under aseptic conditions, 10 pieces of Phellinus fungi with a diameter of 0.6cm round cakes were placed in the liquid medium and cultured with intermittent shaking. Under sterile dark...

Embodiment 4

[0067] Optimization of the quality improvement of the solid plate medium of Phellinus chinensis: Under sterile conditions, after inoculating the activated Phellinus slant mother species on the solid plate medium of Phellinus chinensis, cultured at 25°C for 10 days to improve the quality, select Fast growing, dense and vigorous, solid Phellinus strains with neat edges for use.

[0068] The ingredients used in the solid plate medium are: 220g / L potato, 20g / L glucose, 0.4g / L lignin, 1.3g / LMgSO 4 , 1.6g / LKH 2 PO 4 , 3.0ml / L wood vinegar, 16.0g / L agar, the balance of water, and adjust the pH to 6.0.

[0069] Phellinus liquid strain intermittent expansion culture: liquid expansion medium at a temperature of 121 ° C, autoclaved for 30 minutes, and cooled in a sterile environment. Under aseptic conditions, 10 pieces of Phellinus fungi with a diameter of 0.6cm round cakes were placed in the liquid medium and cultured with intermittent shaking. Under sterile dark conditions, the temp...

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