A micro-area Mössbauer spectrometer for ultrafine particle samples and a method for using the same

Abstract

The present invention discloses a micro-area Mössbauer spectrometer for ultrafine particle samples and a method for using the same, which relates to the field of Mössbauer spectroscopy and comprises a base, wherein a vibration sensor is mounted on the upper end of the base, and a bracket is mounted on one side of the base, wherein the bracket is a cylindrical structure, and a sleeve frame is sleeved on one end of the bracket away from the base, wherein the sleeve frame is a cylindrical hollow structure, and openings are provided at the upper and lower ends of the sleeve frame, and a microscope is fixedly connected to one side of the sleeve frame, and a detector is mounted at the lower end of the microscope, and a slide rail is mounted on one side of the detector, and the detector is slidably connected to the slide rail. The micro-area Mössbauer spectrometer for ultrafine particle samples and a method for using the same can drive the adjustment gear to rotate by rotating the adjustment knob, and can drive the latch and the rack to move alternately, and can drive the first fixed rod and the second fixed rod to open outwards, or to close inwards, so as to adjust the angle of the capillary focusing lens.

Description

Technical Field

[0001] The invention discloses a micro-area Mossbauer spectrometer for ultrafine particle samples, in particular to a micro-area Mossbauer spectrometer for ultrafine particle samples and a use method thereof. Background Art

[0002] The Mössbauer spectrometer is a set of instruments made based on the principle of the resonance absorption of gamma rays by recoilless atomic nuclei. It is a uniformly accelerated Mössbauer spectrometer that measures the resonance absorption spectrum. It can also study the intrinsic connection between the microscopic and macroscopic magnetism of magnetic materials under an external magnetic field, increase the research function of spin structure and spin dynamics, and can be used to study the electric or magnetic interaction between the atomic nucleus and its surrounding environment in solid materials such as magnetic materials and high-temperature superconductors. Further study the microscopic magnetism, electronic structure, valence state and distribution of surrounding atoms, as well as the properties of lattice dynamics and lattice relaxation. The Mössbauer spectrometer has the advantage of extremely high energy resolution (the highest energy resolution is 10-13eV), and is one of the powerful means to study the hyperfine interaction and microstructural properties of matter.

[0003] At present, the measurable samples of the Mössbauer spectrometer, whether under high temperature or low temperature conditions, or under backscattering mode test conditions, require relatively large samples, with a sample diameter of not less than 10 mm and a thickness of not less than 0.2 mm depending on the iron content of the sample. If the sample is too thick, it cannot penetrate the sample and there will be self-absorption. If the sample is too thin, the Mössbauer effect will not be obvious, because the excitation ray energy of the Mössbauer spectrometer is 14.4KeV, which is a low-energy gamma ray and has a weak ability to penetrate the sample.

[0004] For many customers who require the measurement of trace samples, or samples in nanogram quantities (fine chemical analysis industry), the existing Mössbauer spectrometer cannot measure such a small amount of samples. For the Mössbauer spectrum test of tiny grains (such as geological samples), it is even more impossible to measure and analyze the Mössbauer spectrum.

[0005] In order to solve the problem of the existing Mössbauer spectroscopy technology requiring a large number of test samples, we have redesigned the measurement optical path and instrument structure of the Mössbauer spectroscopy, which can not only improve the original technical performance, but also measure and analyze nanogram quantities of samples, and can also measure the Mössbauer spectra of micron-level crystal particles in the samples. Combined with optical microscopes and high-energy resolution detectors, as well as X-ray capillary focusing technology, it perfectly solves the new test requirements for samples and test environment conditions faced by the application of Mössbauer spectroscopy in the forefront of modern science. Summary of the invention

[0006] The object of the present invention is to provide a micro-area Mössbauer spectrometer for ultrafine particle samples and a method for using the same, so as to solve the problems raised in the above background technology.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A micro-area Mössbauer spectrometer for ultrafine particle samples and a method for using the same, comprising a base, a vibration sensor being mounted on the upper end of the base, and a bracket being mounted on one side of the base, the bracket being a cylindrical structure, and a sleeve frame being sleeved on one end of the bracket away from the base, the sleeve frame being a cylindrical hollow structure with openings at both ends, a microscope being fixedly connected to one side of the sleeve frame, a detector being mounted just below the microscope, a slide rail being mounted on one side of the detector, and the detector being slidably connected to the slide rail, one end of the slide rail being slidably connected to the detector, and the other end of the slide rail being fixedly connected to the bracket, a carrier being mounted on the lower end of the detector, a glass slide being clamped inside the carrier, and one side of the carrier being fixedly connected to the bracket, a through hole being opened in the middle of the carrier, and a capillary focusing lens being mounted just below the through hole of the carrier, the capillary focusing lens being of irregular shape, and an adjustment component being mounted in the middle of the capillary focusing lens.

[0009] As a further solution of the present invention: the adjustment assembly includes a fixed frame, the inner wall of the fixed frame is rotatably connected to the first fixed rod and the second fixed rod respectively through a rotating shaft, the first fixed rod and the second fixed rod are divided into two groups of identical symmetrical long rods, the two groups of the first fixed rods are rotatably connected through a rotating shaft and a connecting rod, the two groups of the second fixed rods are rotatably connected through a rotating shaft and a connecting rod, and the first fixed rod and the second fixed rod are rotatably connected through a connecting sleeve.

[0010] As a further solution of the present invention: the internal movable connection of the fixing frame is provided with a latch tooth and a rack, the end of the latch tooth away from the fixing frame is fixedly connected to the outer surface of the first fixing rod, the end of the rack away from the fixing frame is fixedly connected to the outer surface of the second fixing rod, and the latch tooth and the rack are both semi-arc-shaped.

[0011] As a further solution of the present invention: a gear is rotatably connected to the interior of the fixing frame via a rotating shaft, and the gear is meshed with the latch teeth and the rack.

[0012] As a further solution of the present invention: the front end of the adjusting gear is connected to an adjusting knob via a threaded rod, and the adjusting knob is arranged on the front end of the fixing frame, and the adjusting knob is rotatably connected to the fixing frame.

[0013] As a further solution of the present invention: the vibration sensor is composed of four groups of driving modules, and a Mössbauer source is installed in the middle position of the vibration sensor, a capillary focusing lens is sleeved on the outside of the Mössbauer source, one end of the capillary focusing lens is placed inside the vibration sensor, and the other end of the capillary focusing lens is placed inside the carrier.

[0014] As a further solution of the present invention: a glass cover is installed at the lower end of the base, the base and the bracket are both installed at the bottom end of the inner wall of the glass cover, a groove is opened at the bottom end of the inner wall of the glass cover, and the base is placed in the groove, the base is connected to the bottom end of the groove through a spring, one end of the spring is fixedly connected to the bottom end of the groove, and the other end of the spring is fixedly connected to the bottom end of the base.

[0015] As a further solution of the present invention: a camera module is installed on the upper end of the microscope, and a housing of the camera module is fixedly connected to the upper end surface of the microscope.

[0016] As a further solution of the present invention: comprising the following production steps:

[0017] Step 1: Use a blank glass slide to apply fluorescent liquid on one side;

[0018] Step 2: Use the "+" on the microscope to center the image and make sure the focus is at the center of the microscope focal plane;

[0019] Step 3: Raise the microscope by 5 cm and move the Si-Pin crystal detector above the microscope focal plane;

[0020] Step 4: Start measuring and collecting Musspectr spectral data.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. The present invention: by rotating the adjusting knob to drive the adjusting gear to rotate, the latch teeth and the rack can be driven to move alternately, and the first fixing rod and the second fixing rod can be driven to open outward or close inward to adjust the angle of the capillary focusing lens. A groove is provided between the bottom end of the base and the bottom end of the inner wall of the glass cover, and a spring is installed inside the groove, and the spring rebound is used to increase the buffer.

[0023] 2. The present invention can measure the Mössbauer spectrum of samples in the nanogram order in a temperature environment of 1.5K to 300K.

[0024] 3. The present invention can measure the Mössbauer spectrum of a crystal particle sample with a diameter of 2 to 5 um in a temperature environment of 1.5K to 300K.

[0025] 4. The present invention: The energy measurement resolution of the Mössbauer spectrum of the sample can reach 200 eV, or even lower. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram of the present invention;

[0027] Figure 2 It is a schematic diagram of the top view of the structure of the adjustment component in the present invention;

[0028] Figure 3 It is a side view structural schematic diagram of the adjustment knob in the present invention;

[0029] Figure 4 A top view of the structure of the gear in the present invention;

[0030] Figure 5 It is a schematic diagram of the three-dimensional structure of the base in the present invention.

[0031] In the figure: 1. camera module; 2. microscope; 3. detector; 4. stage; 5. capillary focusing lens; 6. vibration sensor; 7. drive module; 8. glass cover; 9. groove; 10. sleeve; 11. slide rail; 12. bracket; 13. slide; 14. Mössbauer source; 15. base; 16. adjustment knob; 17. fixing frame; 18. rack; 19. adjustment assembly; 20. latch; 21. first fixing rod; 22. second fixing rod; 23. connecting rod; 24. rotating shaft; 25. connecting sleeve; 26. threaded rod; 27. gear; 28. spring. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] See also Figures 1 to 5In the embodiment of the present invention, a micro-area Mössbauer spectrometer for ultrafine particle samples and a method for using the same include a base 15, a vibration sensor 6 is installed on the upper end of the base 15, and a bracket 12 is installed on one side of the base 15, the bracket 12 is a cylindrical structure, and a sleeve 10 is sleeved on the end of the bracket 12 away from the base 15, the sleeve 10 is a cylindrical hollow structure with openings at both ends, a microscope 2 is fixedly connected to one side of the sleeve 10, a detector 3 is installed at the lower end of the microscope 2, and a sliding The detector 3 is slidably connected to the slide rail 11, one end of the slide rail 11 is slidably connected to the detector 3, and the other end of the slide rail 11 is fixedly connected to the bracket 12, a carrier 4 is installed at the lower end of the detector 3, a slide glass 13 is clamped inside the carrier 4, and one side of the carrier 4 is fixedly connected to the bracket 12, a through hole is opened in the middle of the carrier 4, and a capillary focusing lens 5 is installed at the lower end of the through hole of the carrier 4, the capillary focusing lens 5 is irregular in shape, and an adjustment component 19 is installed in the middle of the capillary focusing lens 5.

[0034] The adjustment assembly 19 includes a fixed frame 17, the inner wall of the fixed frame 17 is rotatably connected to the first fixed rod 21 and the second fixed rod 22 respectively through a rotating shaft 24, the first fixed rod 21 and the second fixed rod 22 are divided into two groups of identical symmetrical long rods, the two groups of the first fixed rods 21 are rotatably connected through a rotating shaft 24 and a connecting rod 23, the two groups of the second fixed rods 22 are rotatably connected through a rotating shaft 24 and a connecting rod 23, and the first fixed rod 21 and the second fixed rod 22 are rotatably connected through a connecting sleeve 25.

[0035] The fixing frame 17 is internally movably connected with a latch tooth 20 and a rack 18. The end of the latch tooth 20 away from the fixing frame 17 is fixedly connected to the outer surface of the first fixing rod 21, and the end of the rack 18 away from the fixing frame 17 is fixedly connected to the outer surface of the second fixing rod 22. The latch tooth 20 and the rack 18 are both semi-arc-shaped.

[0036] The interior of the fixing frame 17 is rotatably connected to a gear 27 via a rotating shaft 24 , and the gear 27 is meshed with the latching teeth 20 and the rack 18 .

[0037] The front end of the adjusting gear 27 is connected to the adjusting knob 16 via a threaded rod 26 , and the adjusting knob 16 is disposed at the front end of the fixing frame 17 , and the adjusting knob 16 is rotatably connected to the fixing frame 17 .

[0038] The vibration sensor 6 is composed of four groups of driving modules 7, and a Mössbauer source 14 is installed in the middle position of the vibration sensor 6. A capillary focusing lens 5 is sleeved on the outer side of the Mössbauer source 14. One end of the capillary focusing lens 5 is placed inside the vibration sensor 6, and the other end of the capillary focusing lens 5 is placed inside the carrier 4.

[0039] A glass cover 8 is installed at the lower end of the base 15, and the base 15 and the bracket 12 are both installed at the bottom end of the inner wall of the glass cover 8. A groove 9 is opened at the bottom end of the inner wall of the glass cover 8, and the base 15 is placed in the groove 9. The base 15 is connected to the bottom end of the groove 9 through a spring 28, one end of the spring 28 is fixedly connected to the bottom end of the groove 9, and the other end of the spring 28 is fixedly connected to the bottom end of the base 15.

[0040] A camera module 1 is installed on the upper end of the microscope 2 , and a housing of the camera module 1 is fixedly connected to the upper end surface of the microscope 2 .

[0041] The steps for using this device are:

[0042] Step 1: Use a blank glass slide 13 to apply fluorescent luminescent liquid on one side;

[0043] Step 2: Use the microscope 2 "+" to center and make sure the focus is at the center of the microscope focal plane;

[0044] Step 3: Raise the microscope 2 by 5 cm and move the Si-Pin crystal detector 3 above the microscope focal plane;

[0045] Step 4: Start measuring and collecting Musspectr spectral data.

[0046] The preferred embodiments of the present invention are:

[0047] A micro-area Mössbauer spectrometer for ultrafine particle samples and a method for using the same, comprising a base 15, wherein a vibration sensor 6 is mounted on the upper end of the base 15, and a bracket 12 is mounted on one side of the base 15, wherein the bracket 12 is a cylindrical structure, and a sleeve 10 is sleeved on one end of the bracket 12 away from the base 15, wherein the sleeve 10 is a cylindrical hollow structure with openings at both ends, wherein a microscope 2 is fixedly connected to one side of the sleeve 10, wherein a detector 3 is mounted at the lower end of the microscope 2, and a slide rail 11 is mounted on one side of the detector 3, and The detector 3 is slidably connected to the slide rail 11, one end of the slide rail 11 is slidably connected to the detector 3, and the other end of the slide rail 11 is fixedly connected to the bracket 12, a carrier 4 is installed at the lower end of the detector 3, a glass slide 13 is clamped inside the carrier 4, and one side of the carrier 4 is fixedly connected to the bracket 12, a through hole is opened in the middle position of the carrier 4, and a capillary focusing lens 5 is installed at the lower end of the through hole position of the carrier 4, the capillary focusing lens 5 is irregular in shape, and an adjustment component 19 is installed in the middle position of the capillary focusing lens 5.

[0048] Detector 3 is a Si-Pin crystal detector with a resolution of 150kev and an operating temperature of -40°C to +75°C.

[0049] The adjustment assembly 19 includes a fixed frame 17, the inner wall of the fixed frame 17 is rotatably connected to the first fixed rod 21 and the second fixed rod 22 respectively through a rotating shaft 24, the first fixed rod 21 and the second fixed rod 22 are divided into two groups of identical symmetrical long rods, the two groups of the first fixed rods 21 are rotatably connected through a rotating shaft 24 and a connecting rod 23, the two groups of the second fixed rods 22 are rotatably connected through a rotating shaft 24 and a connecting rod 23, and the first fixed rod 21 and the second fixed rod 22 are rotatably connected through a connecting sleeve 25.

[0050] The fixing frame 17 is internally movably connected with a latch tooth 20 and a rack 18. The end of the latch tooth 20 away from the fixing frame 17 is fixedly connected to the outer surface of the first fixing rod 21, and the end of the rack 18 away from the fixing frame 17 is fixedly connected to the outer surface of the second fixing rod 22. The latch tooth 20 and the rack 18 are both semi-arc-shaped.

[0051] The interior of the fixing frame 17 is rotatably connected to a gear 27 via a rotating shaft 24 , and the gear 27 is meshed with the latching teeth 20 and the rack 18 .

[0052] The front end of the adjusting gear 27 is connected to the adjusting knob 16 via a threaded rod 26 , and the adjusting knob 16 is disposed at the front end of the fixing frame 17 , and the adjusting knob 16 is rotatably connected to the fixing frame 17 .

[0053] The vibration sensor 6 is composed of four groups of driving modules 7, and a Mössbauer source 14 is installed in the middle of the vibration sensor 6. A capillary focusing lens 5 is sleeved on the outer side of the Mössbauer source 14. One end of the capillary focusing lens 5 is placed inside the vibration sensor 6, and the other end of the capillary focusing lens 5 is placed inside the carrier 4. When working, the adjustment knob 16 is rotated to drive the adjustment gear 27 to rotate, which can drive the latch 20 and the rack 18 to move alternately, and can drive the first fixed rod 21 and the second fixed rod 22 to open outward or close inward to adjust the angle of the capillary focusing lens 5. Thus, the capillary focusing lens 5 focuses the Mössbauer source 14, and the capillary focusing lens 5 collects a large solid angle of X-rays from the Mössbauer source 14 and focuses the rays. After focusing, the focal diameter is: 2-10um, preferably 6um, preferably 8um; incident ray capture angle: 0.05-0.2rad; front and rear focal distance: 175mm; focusing device outer diameter: 15-20mm; intensity gain: 500-2000. Through the above settings, more accurate measurement values ​​can be obtained to achieve the measurement of ultrafine particle samples.

[0054] A glass cover 8 is installed at the lower end of the base 15. The base 15 and the bracket 12 are both installed at the bottom of the inner wall of the glass cover 8. A groove 9 is opened at the bottom of the inner wall of the glass cover 8, and the base 15 is placed in the groove 9. The base 15 is connected to the bottom of the groove 9 through a spring 28. One end of the spring 28 is fixedly connected to the bottom of the groove 9, and the other end of the spring 28 is fixedly connected to the bottom of the base 15. During operation, the vibration sensor 6 and the drive module 7 increase the buffering through the rebound of the spring 28. The preferred vibration sensor 6 has a speed range of 0-±300mm / s, a resonance frequency of ~25Hz, an accuracy of ±0.05% (sine wave mode), and a linearity of better than 0.15% at a speed of up to ±100mm / s, thereby achieving a more preferred measurement accuracy.

[0055] No shielding lead sheet is arranged in the glass cover 8 or shielding lead sheet is arranged, the shielding lead sheet contains 99.5% lead, the thickness of the shielding lead sheet is 5mm, and the shielding lead sheet is about 2 square meters.

[0056] The camera module 1 is installed on the upper end of the microscope 2, and the housing of the camera module 1 is fixedly connected to the upper end surface of the microscope 2. The camera module 1 can be a digital camera or computer imaging software, and is set to a resolution of at least 8 million pixels when working.

[0057] The preferred steps are:

[0058] Step 1: Set the working environment temperature: 5-35℃; working relative humidity: 20-90%; system power supply voltage requirements: meet domestic power grid standards, 220VAC±10% / single-phase 5KW; use a shock-absorbing platform desktop layout.

[0059] Step 2: Use a blank glass slide 13 to apply fluorescent luminescent liquid on one side;

[0060] Step 3: Use the microscope 2 "+" to center and make sure the focus is at the center of the microscope focal plane;

[0061] Step 4: Raise the microscope 2 by 5 cm and move the Si-Pin crystal detector 3 above the microscope focal plane;

[0062] Step 5: Start measuring and collecting Musspectr spectral data.

[0063] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0064] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A micro-area Mössbauer spectrometer for ultrafine particle samples, comprising a base (15), characterized in that: A vibration sensor (6) is installed at the upper end of the base (15), and a bracket (12) is installed at one side of the base (15); the bracket (12) is a cylindrical structure, and a sleeve frame (10) is sleeved at one end of the bracket (12) away from the base (15); the sleeve frame (10) is a cylindrical hollow structure, and openings are provided at the upper and lower ends of the sleeve frame (10); a microscope (2) is fixedly connected to one side of the sleeve frame (10); a detector (3) is installed at the lower end of the microscope (2); a slide rail (11) is installed at one side of the detector (3), and the detector (3) slides with the slide rail (11). The detector (3) is connected to the slide rail (11), one end of the slide rail (11) is slidably connected to the detector (3), and the other end of the slide rail (11) is fixedly connected to the bracket (12), a carrier (4) is installed at the lower end of the detector (3), a glass slide (13) is clamped inside the carrier (4), and one side of the carrier (4) is fixedly connected to the bracket (12), a through hole is opened in the middle of the carrier (4), and a capillary focusing lens (5) is installed at the lower end of the through hole of the carrier (4), the capillary focusing lens (5) is irregular in shape, and an adjustment component (19) is installed in the middle of the capillary focusing lens (5); The vibration sensor (6) is composed of four groups of driving modules (7), and a Mössbauer source (14) is installed in the middle of the vibration sensor (6). A capillary focusing lens (5) is sleeved on the outer side of the Mössbauer source (14), one end of the capillary focusing lens (5) is placed inside the vibration sensor (6), and the other end of the capillary focusing lens (5) is placed inside the carrier (4).

2. The micro-area Mössbauer spectrometer for ultrafine particle samples according to claim 1, characterized in that: The adjustment assembly (19) comprises a fixing frame (17), the inner wall of the fixing frame (17) being rotatably connected to a first fixing rod (21) and a second fixing rod (22) respectively via a rotating shaft (24), the first fixing rod (21) and the second fixing rod (22) being divided into two groups of identical symmetrical long rods, the two groups of the first fixing rods (21) being rotatably connected to each other via a rotating shaft (24) and a connecting rod (23), the two groups of the second fixing rods (22) being rotatably connected to each other via a rotating shaft (24) and a connecting rod (23), and the first fixing rod (21) and the second fixing rod (22) being rotatably connected to each other via a connecting sleeve (25).

3. The micro-area Mössbauer spectrometer for ultrafine particle samples according to claim 2, characterized in that: The fixing frame (17) is internally movably connected with a latch tooth (20) and a rack (18); one end of the latch tooth (20) away from the fixing frame (17) is fixedly connected to the outer surface of a first fixing rod (21); one end of the rack (18) away from the fixing frame (17) is fixedly connected to the outer surface of a second fixing rod (22); and the latch tooth (20) and the rack (18) are both semi-arc shaped.

4. The micro-area Mössbauer spectrometer for ultrafine particle samples according to claim 3, characterized in that: The interior of the fixing frame (17) is rotatably connected to a gear (27) via a rotating shaft (24), and the gear (27) is meshed with the latching teeth (20) and the rack (18).

5. The micro-area Mössbauer spectrometer for ultrafine particle samples according to claim 4, characterized in that: The front end of the adjusting gear (27) is connected to an adjusting knob (16) via a threaded rod (26), and the adjusting knob (16) is arranged at the front end of a fixing frame (17), and the adjusting knob (16) is rotatably connected to the fixing frame (17).

6. The micro-area Mössbauer spectrometer for ultrafine particle samples according to claim 1, characterized in that: A glass cover (8) is installed at the lower end of the base (15); the base (15) and the bracket (12) are both installed at the bottom end of the inner wall of the glass cover (8); a groove (9) is provided at the bottom end of the inner wall of the glass cover (8); the base (15) is placed in the groove (9); the base (15) and the bottom end of the groove (9) are connected via a spring (28); one end of the spring (28) is fixedly connected to the bottom end of the groove (9), and the other end of the spring (28) is fixedly connected to the bottom end of the base (15).

7. The micro-area Mössbauer spectrometer for ultrafine particle samples according to claim 1, characterized in that: A camera module (1) is installed on the upper end of the microscope (2), and the outer shell of the camera module (1) is fixedly connected to the upper end surface of the microscope (2).

8. A method for using a micro-area Mössbauer spectrometer for ultrafine particle samples according to any one of claims 1 to 7, characterized in that: The following steps are involved: Step 1: Use a blank glass slide (13) to apply fluorescent luminescent liquid on one side; Step 2: Use the "+" centering function on the microscope (2) to ensure that the focus is at the center of the microscope focal plane; Step 3: Raise the microscope (2) by 5 cm and move the Si-Pin crystal detector (3) above the microscope focal plane; Step 4: Start measuring and collecting Musspectr spectral data.

Images