A pre-processing device for thromboelastograph

Abstract

The present application discloses a pre-treatment device for a thromboelastograph. The pre-treatment device includes: a sampling device, which is used to connect with a sample system to be injected, so that the sample in the sample system to be injected is transported to the pre-treatment device via the sampling device; a goose-shaped tube, which is in fluid communication with the sampling device; a sample temporary storage chamber, in which a sample inlet and a sample outlet are provided, and the sample inlet is connected to the goose-shaped tube; the sample temporary storage chamber is also provided with one or more vents, and positive pressure or negative pressure is generated in the sample temporary storage chamber through the vents; a one-way valve mechanism, in which one side of the one-way valve mechanism is connected to the sample outlet of the sample temporary storage chamber, and the other side is connected to the detection chamber of the thromboelastograph. The pre-treatment device can replace the manual reagent addition operation of the currently available thromboelastograph, and adopts the positive pressure / negative pressure change method to realize the automated operation process of detecting samples.

Description

Technical Field

[0001] The present application relates to a detection instrument, and in particular to a pre-processing device for a thromboelastograph. Background Art

[0002] Thrombosis and thromboembolism are the direct causes of death, and are also key factors and important links in the occurrence of cardiovascular and cerebrovascular events. In recent years, disease management programs (DMPs) have been highly praised as solutions to chronic diseases such as diabetes, heart disease and asthma. However, due to a series of weaknesses in conventional disease management methods (clinical laboratories / clinical monitoring), faster, simpler and more accurate monitoring equipment is needed to replace them. In this context, the field of point-of-care testing (POCT) has gained huge room for development. In 2015, the global in vitro diagnostic market reached US$60 billion, of which the market share related to coagulation testing reached US$1.8 billion.

[0003] Coagulation analyzers are widely used in the field of POCT coagulation testing, and each indicator has clear clinical and physiological significance. Thromboelastography has also been widely promoted.

[0004] Thrombelastogram (TEG) is a graph drawn by a thromboelastometry instrument, which is an indicator that reflects the dynamic changes of blood coagulation (including the formation rate of fibrin, the dissolution state and the firmness and elasticity of the coagulation). Although the thromboelastometry instrument has a series of advanced, convenient and innovative features in detection methods, detection processes and detection accuracy, it is based on its inherent characteristics under the premise of its own detection principle. The current thromboelastometry instrument still has a series of shortcomings and deficiencies in terms of patient experience, detection efficiency, degree of automation, etc., which greatly limits its application prospects in hospitals, professional clinical testing institutions, and especially in the field of patient home health monitoring. Summary of the invention

[0005] The present invention mainly aims at various problems existing in the currently available thromboelastographs on the market. It adopts an integrated and modular design, fixes the detection reagents in the sample pretreatment device, realizes the automated operation of the detection samples (from the sample injection system to the detection chamber), and realizes the complete detection process of the thromboelastograph on the basis of the existing detection principle unchanged.

[0006] The present application provides a pre-processing device for a thromboelastograph, comprising:

[0007] A sample injection device, used to connect with the system to be injected, so that the sample in the system to be injected is transported to the pre-treatment equipment via the sample injection device;

[0008] a goose-shaped tube, the goose-shaped tube being in fluid communication with the injection device;

[0009] A sample temporary storage chamber, wherein the sample temporary storage chamber is provided with a sample inlet and a sample outlet, wherein the sample inlet is connected to the goose-shaped tube; the sample temporary storage chamber is also provided with one or more vents, through which positive pressure or negative pressure is generated in the sample temporary storage chamber;

[0010] A one-way valve mechanism, one side of which is connected to the sample outlet of the sample temporary storage chamber, and the other side of which is connected to the detection chamber of the thromboelastograph.

[0011] In one embodiment, a U-shaped buffer tube is provided between the injection device and the goose-shaped tube, and its purpose is twofold: to limit the depth of the injection needle entering the sample tube (chip) and to slow down the actuation of the sample at the moment of sample tube insertion and valve opening.

[0012] In one embodiment, the sample temporary storage chamber is connected to a pressure system through the vent.

[0013] In one embodiment, the pressure system includes an air pump, a positive pressure tank and a negative pressure tank, the air pump is connected to the positive pressure tank and the negative pressure tank respectively, the positive pressure tank is connected to the vent through a positive pressure valve, and the negative pressure tank is connected to the vent through a negative pressure valve.

[0014] In one embodiment, the sample temporary storage chamber is further provided with a reagent area for storing reagents near the sample outlet.

[0015] In one embodiment, a reagent inlet is further provided in the sample temporary storage chamber for delivering reagents to the reagent area.

[0016] In one embodiment, the sample inlet is arranged at the upper part of the sample temporary storage chamber, and the sample outlet is arranged at the lower part of the sample temporary storage chamber.

[0017] In one embodiment, the pre-treatment device further includes a sample injection detector, and the sample injection detector is arranged between the one-way valve mechanism and the sample temporary storage chamber.

[0018] In one embodiment, the sampling device is a blood collection tube needle.

[0019] In one embodiment, the detection chamber includes a cup body and a cup cover, and the lower part of the cup body is connected to the one-way valve mechanism.

[0020] The pre-treatment device of the present application can replace the manual reagent addition operation of the currently available thromboelastograph, and adopts the positive / negative pressure change method to realize the automated operation process of detecting samples. The pre-treatment device of the present application also has the following advantages:

[0021] 1) Avoid manual operation errors and improve the consistency of sample operation;

[0022] 2) This equipment is a closed system, which can reduce the risk of infection to operators;

[0023] 3) After the test is completed, simplify the process of handling discarded samples;

[0024] 4) Reduce the amount and cost of consumables (reagents are enclosed in the chip) and reduce the contamination of samples caused by exposure to the environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 A schematic diagram of the pre-treatment equipment of the present application is shown;

[0026] Figure 2 A schematic diagram of the pressure system is shown;

[0027] Figure 3 A schematic structural diagram of a one-way valve mechanism according to an embodiment of the present application is shown;

[0028] Figure 4 Show Figure 3 An illustration of the one-way valve mechanism shown when the lower chamber is under negative pressure;

[0029] Figure 5 Show Figure 3 An illustration of the one-way valve mechanism shown when the lower chamber is under positive pressure;

[0030] Figure 6 The figure shows the situation when the lower cavity in the one-way valve mechanism generates positive pressure, showing the opening of the communication passage between the upper cavity and the lower cavity;

[0031] Figure 7 The figure shows the situation when the lower cavity in the one-way valve mechanism generates negative pressure (or no pressure), showing the closing of the communication passage between the upper cavity and the lower cavity;

[0032] Figure 8 A schematic structural diagram of a one-way valve mechanism according to an embodiment is shown. DETAILED DESCRIPTION

[0033] The present application is further described in detail below through the accompanying drawings and embodiments. Through these descriptions, the characteristics and advantages of the present application will become clearer and more specific.

[0034] The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration." Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments. Although various aspects of the embodiments are shown in the drawings, the drawings are not necessarily drawn to scale unless otherwise noted.

[0035] In addition, the technical features involved in different embodiments of the present application described below can be combined with each other as long as they do not conflict with each other.

[0036] The present application provides a pre-treatment device for a thromboelastograph, which can be connected to the detection chamber of the thromboelastograph, so that the sample to be tested is transported to the detection chamber of the thromboelastograph through the pre-treatment device for detection by the thromboelastograph.

[0037] like Figure 1-2 As shown, the pre-processing equipment provided by this application includes:

[0038] The sample injection device 1 is used to connect with the system to be injected, so that the sample in the system to be injected is transported to the pre-treatment device via the sample injection device 1;

[0039] A goose-shaped tube 2, wherein the goose-shaped tube is in fluid communication with the sample injection device 1;

[0040] A sample temporary storage chamber 3, wherein a sample inlet 31 and a sample outlet 32 ​​are provided in the sample temporary storage chamber, wherein the sample inlet 31 is connected to the goose-shaped tube 2; the sample temporary storage chamber 3 is also provided with one or more vents 33, through which positive pressure or negative pressure is generated in the sample temporary storage chamber 3;

[0041] A one-way valve mechanism 4 , one side of which is connected to the sample outlet 32 ​​of the sample temporary storage chamber, and the other side of which is connected to the detection chamber 8 of the thromboelastography instrument.

[0042] In the pre-treatment device of the present application, the sampling device 1 can be various sampling devices in the art, such as pipelines, sampling needles, etc. However, the pre-treatment device of the present application is used for a thromboelastograph, and the sample to be processed is usually a blood sample. In one embodiment, the system to be sampled is a blood collection tube, and the sampling device 1 is a blood collection tube insertion needle, which is used to directly connect to the blood collection tube (the system to be sampled), and the blood sample in the blood collection tube can be directly transported to the final thromboelastograph through the pre-treatment device of the present application for corresponding detection.

[0043] The pre-treatment device of the present application includes a goose-shaped tube 2, which is a microfluidic pipeline, and its function is to prevent the sample in the sample chamber from flowing back when the pipeline is under positive pressure. Although it is theoretically possible to directly connect the sample temporary storage bin and the injection needle (for example, the injection needle is placed directly above the temporary storage bin), since an air path system must be set up at this position (the space on the chip is too small), and it also needs to be coordinated with the internal structure of the original detection equipment, the current layout is adopted.

[0044] In one embodiment, a U-shaped buffer tube 5 is provided between the injection device 1 and the goose-shaped tube 2, which has two purposes: to limit the depth of the injection needle entering the sample tube, and to slow down the actuation of the sample at the moment of sample tube insertion and valve opening.

[0045] The pre-treatment device of the present application includes a sample temporary storage chamber 3, wherein the sample temporary storage chamber 3 is provided with a sample inlet 31 and a sample outlet 32, wherein the sample inlet 31 is connected to the goose-shaped tube 2; the sample temporary storage chamber 3 is also provided with one or more vents 33, through which positive pressure or negative pressure is generated in the sample temporary storage chamber 3. The sample inlet 31 is connected to the goose-shaped tube 2, and is used to input the sample into the sample temporary storage chamber 3 through the sample inlet 31; and the purpose of providing the sample outlet 32 ​​is to discharge the sample in the sample temporary storage chamber 3 from the sample temporary storage chamber 3.

[0046] The sample temporary storage chamber is actually a chamber for temporarily storing samples. The "reagent" can be placed in the chamber in advance, and the sample will be mixed and dissolved with the reagent when it enters the chamber. The purpose of this chamber is mainly twofold: 1) to ensure sufficient sample injection volume, so that enough sample volume can enter the final detection chamber; 2) in this chamber, the sample and the reagent are mixed (the time required for this mixing is not strict, for example, it can be greater than 1 minute and less than 5 minutes). The size and shape of the sample temporary storage chamber can be selected according to specific needs.

[0047] In the present application, the way of inputting and outputting the sample into and out of the sample temporary storage chamber 3 can be realized by the pressure change in the sample temporary storage chamber 3. The sample temporary storage chamber 3 is also provided with one or more vents 33, through which positive pressure or negative pressure is generated in the sample temporary storage chamber 3. When negative pressure is generated in the sample temporary storage chamber 3, the sample is input into the sample temporary storage chamber 3 through the sample inlet 31; when positive pressure is generated in the sample temporary storage chamber 3, the sample is output from the sample temporary storage chamber 3 through the sample outlet 32. For this reason, the sample inlet 31 is arranged at the upper part of the sample temporary storage chamber 3, and the sample outlet 32 ​​is arranged at the lower part of the sample outlet 32. In addition, the sample amount in the sample temporary storage chamber 3 is controlled not to exceed the sample inlet 31, so that when positive pressure is generated in the sample temporary storage chamber 3, the sample can only be output from the sample temporary storage chamber 3 through the sample outlet 32, and will not be output from the sample inlet 31.

[0048] In one embodiment, the sample storage chamber 3 is connected to the pressure system through the vent 33. Figure 2The pressure system is shown, and the pressure system includes an air pump 63, a positive pressure tank 62 and a negative pressure tank 65. The air pump 63 is connected to the positive pressure tank 62 and the negative pressure tank 65 respectively. The positive pressure tank 62 is connected to the vent 33 through a positive pressure valve 61, and the negative pressure tank 65 is connected to the vent 33 through a negative pressure valve 64. The positive and negative pressures are generated by the positive pressure tank 62 and the negative pressure tank 65 in cooperation with the air pump 63: the air pump 63 pumps air from the "negative pressure tank 65" to the "positive pressure tank 62", which is equivalent to continuously pumping air into the positive pressure tank 62. When the air pressure in the positive pressure tank 62 is sufficient, the positive pressure valve 61 opens, and the gas is discharged into the sample temporary storage chamber 3, thereby forming a positive pressure in the sample temporary storage chamber 3; when the air pressure in the negative pressure tank 65 is sufficient, the negative pressure valve 64 opens, and the gas is sucked from the sample temporary storage chamber 3, thereby forming a negative pressure in the sample temporary storage chamber 3. In one embodiment, the pressure system can be directly connected to the vent 33, so two vents 33 can be set in the sample temporary storage chamber 3, one vent is connected to the positive pressure valve 61, and the other vent is connected to the negative pressure valve 64. In a preferred embodiment, the sample temporary storage chamber 3 is provided with one vent 33, and a positive pressure interface 331 and a negative pressure interface 332 are provided on the pipeline connected to the vent 33; Figure 2 As shown, the positive pressure valve 61 is connected to the positive pressure interface 331, and the negative pressure valve 64 is connected to the negative pressure interface 332; this method only requires one vent to be provided on the sample temporary storage chamber 3, and the production of the sample temporary storage chamber 3 is simpler, so it is preferred.

[0049] In one embodiment, the sample temporary storage chamber 3 is also provided with a reagent area 34 for storing reagents near the sample outlet. In one embodiment, the reagent area 34 is equipped with reagents for thromboelastography measurement, including blood coagulation activators such as kaolin, and other reagents such as heparinase, batroxobin, factor XIII, etc. The kaolin is fixed by a liquid drop-freeze drying method (in fact, it can also be not freeze-dried, and directly dripped on the reagent area 34, and the reagent area 34 is located in the lower left area of ​​the sample temporary storage chamber 3). When the blood enters the reagent area through the goose-shaped tube 2, it is mixed with the kaolin reagent under the action of power. This process is relatively easy (for example, the amount of kaolin is only 10ul, and the blood is about 400ul, and this dissolution process is relatively fast). In one embodiment, a reagent inlet (not shown) can also be provided in the sample temporary storage chamber 3 for transporting reagents to the reagent area.

[0050] The pre-treatment device further includes a one-way valve mechanism 4, one side of which is connected to the sample outlet of the sample temporary storage chamber. When the sample temporary storage chamber 3 maintains a negative pressure, the one-way valve mechanism 4 is closed, so that the sample in the one-way valve mechanism 4 does not flow back into the sample temporary storage chamber 3; when the sample temporary storage chamber 3 maintains a positive pressure, the one-way valve mechanism 4 is opened, so that the sample in the sample temporary storage chamber 3 passes through the one-way valve mechanism 4 under the action of pressure and then provides the sample to the detection chamber 8.

[0051] The pre-treatment device further comprises a sample injection detector 7, which is arranged between the one-way valve mechanism 4 and the sample temporary storage chamber 3. The function of the sample injection detector is to monitor the volume of the sample that finally flows into the detection cup, and to judge whether the required volume of blood has completely flowed through the position through the optical sensor at the position (flowing through the position indicates that it has entered the detection chamber).

[0052] The sample injection detector 7 can have a built-in light bead element to illuminate a specific position in the pipeline between the one-way valve mechanism 4 and the sample temporary storage chamber 3 to detect whether the sample before this position has completely entered the one-way valve mechanism 4 to ensure that the sample entering the one-way valve mechanism 4 reaches the set value.

[0053] As mentioned above, the one-way valve mechanism 4 is connected to the detection chamber 8 of the thromboelastograph, so that the sample from the sample storage chamber can be collected in the detection chamber 8. The detection chamber 8 is taken out, and then the thromboelastograph is used to perform the corresponding test. The final detection process is the same as the conventional thromboelastograph detection process, which will not be repeated here. In one embodiment, the detection chamber 8 is a detection cup, including a cup body and a cup cover, and the lower part of the cup body is connected to the one-way valve mechanism 4, so that the sample from the sample storage chamber 3 is finally transported to the detection chamber 8.

[0054] In one embodiment, when the pre-treatment device of the present application is used to process a blood sample, calcium chloride is also provided in the pre-treatment device. The calcium chloride, like kaolin, is a liquid / dry powder reagent that needs to be built into the sample pre-treatment device (it is an indispensable reagent component for activating blood sample coagulation and cannot be replaced by other reagents). When the blood enters the sample pre-treatment device from the injection needle, it needs to be mixed with kaolin first and then with calcium chloride in order before the blood begins to coagulate. In one embodiment, calcium chloride can be pre-existing in one or more of the following in the form of a solution or dry powder reagent: a pipeline connecting the sample outlet 32 ​​and the one-way valve mechanism 4, a one-way valve mechanism 4, and a detection chamber 8; thus, it is possible to make it no longer necessary to inject calcium chloride reagent during the injection process. In one embodiment, a calcium chloride injection port (not shown) is provided in the pipeline connecting the sample outlet 32 ​​and the one-way valve mechanism 4, so that the calcium chloride reagent can be injected during the injection process.

[0055] The structure of the one-way valve mechanism 4 that can be used in the present application is described below. Figure 3 A schematic structural diagram of a one-way valve mechanism according to an embodiment of the present application is shown; Figure 4 Show Figure 3 An illustration of the one-way valve mechanism shown when the lower chamber is under negative pressure; Figure 5 Show Figure 3 An illustration of the one-way valve mechanism shown when the lower chamber is under positive pressure; Figure 6 The figure shows the situation when the lower cavity in the one-way valve mechanism generates positive pressure, showing the opening of the communication passage between the upper cavity and the lower cavity; Figure 7 The figure shows the situation when the lower cavity in the one-way valve mechanism generates negative pressure (or no pressure), showing the closing of the communication passage between the upper cavity and the lower cavity; Figure 8 A schematic structural diagram of a one-way valve mechanism according to an embodiment is shown.

[0056] like Figure 3-8 As shown, the one-way valve mechanism 4 that can be used in the present application is a microfluidic one-way valve, which includes a one-way valve cavity 10, characterized in that the microfluidic one-way valve also includes:

[0057] A valve plate structure 101 is disposed inside the one-way valve cavity 10, and the valve plate structure 101 divides the interior of the one-way valve cavity 10 into an upper cavity 102 and a lower cavity 103; the valve plate structure 101 is configured to open a communication channel between the upper cavity 102 and the lower cavity 103 in one direction,

[0058] an inlet conduit 104 in fluid communication with the lower cavity 103 of the one-way valve cavity; and

[0059] An outlet pipe 105 is in fluid communication with the upper cavity 102 of the one-way valve cavity.

[0060] like Figure 3-7 As shown, in one embodiment, the lower cavity 103 of the one-way valve cavity is configured to be positive pressure, and the valve plate structure 101 opens the communication channel between the upper cavity 102 and the lower cavity 103. The positive pressure can be generated by a pressure system (not shown) that is in fluid communication with the inlet pipe 104. The pressure system may include an air pump, etc. The pressure system such as the air pump generates positive pressure to the system connected to the inlet pipe 104, and then a positive pressure can be generated in the lower cavity 103 of the one-way valve cavity. Under the positive pressure, the valve plate structure 101 can open the communication channel between the upper cavity 102 and the lower cavity 103, so that the upper cavity 102 is connected to the lower cavity 103, so that the fluid (gas or liquid) in the lower cavity 103 flows into the upper cavity 102 and flows out of the one-way valve through the outlet pipe 105.

[0061] In one embodiment, the lower cavity 103 of the one-way valve cavity is configured to be negative pressure, and the valve plate structure 101 closes the communication channel between the upper cavity 102 and the lower cavity 103. The negative pressure can also be generated by a pressure system (not shown) that is in fluid communication with the inlet pipe 104. The pressure system may include an air pump, etc. The pressure system such as the air pump generates negative pressure to the system connected to the inlet pipe 104, and then negative pressure can be generated in the lower cavity 103 of the one-way valve cavity. Under the negative pressure, the valve plate structure 101 closes the communication channel between the upper cavity 102 and the lower cavity 103, so that the upper cavity 102 and the lower cavity 103 are not connected, so that the fluid (gas or liquid) in the lower cavity 103 cannot flow into the upper cavity 102, and the fluid (gas or liquid) in the upper cavity 102 cannot flow back into the lower cavity 103.

[0062] In one embodiment, the valve plate structure 101 includes a fixing portion 1011 disposed on the inner wall of the one-way valve cavity 10, and a valve plate 1012 connected to the fixing portion 1011, and the valve plate 1012 closes or opens the communication channel between the upper cavity 102 and the lower cavity 103. The valve plate 1012 is connected to the fixing portion 1011 and can rotate around the fixing portion 1011 in the one-way valve cavity 10. At the same time, the valve plate 1012 also contacts the inner wall of the one-way valve cavity 10, and the portion in contact with the inner wall of the one-way valve cavity is adapted to the shape of the inner wall of the one-way valve cavity. Thus, the valve plate 1012 can form contact with the inner wall of the one-way valve cavity under the negative pressure in the lower cavity 103, thereby closing the communication channel between the upper cavity 102 and the lower cavity 103, and the fluid (gas or liquid) in the lower cavity 103 cannot flow into the upper cavity 102. At the same time, the valve plate 1012 can be spaced apart from the inner wall of the one-way valve cavity under the action of the positive pressure in the lower cavity 103, thereby opening a connecting channel between the upper cavity 102 and the lower cavity 103, so that the fluid (gas or liquid) in the lower cavity 103 can flow into the upper cavity 102 through the gap between the valve plate 1012 and the inner wall of the one-way valve cavity.

[0063] In one embodiment, a valve plate baffle is provided on the inner wall of the one-way valve cavity 10, and the valve plate baffle can support the valve plate 1012. At the same time, the upper surface end of the valve plate baffle can match the lower surface end of the valve plate 1012, so that the upper surface of the valve plate baffle and the lower surface of the valve plate can be connected together, so that the upper cavity 102 and the lower cavity 103 are separated by the valve plate structure, thereby closing the communication channel between the two. Moreover, the valve plate baffle and the valve plate can be easily separated, so that the communication channel between the upper cavity 102 and the lower cavity 103 can be opened again. With such a setting, the valve plate can close the communication channel between the upper cavity 102 and the lower cavity 103 under the negative pressure in the lower cavity 103, and the fluid (gas or liquid) in the lower cavity 103 cannot flow into the upper cavity 102. At the same time, the valve plate can also open the communication channel between the upper cavity 102 and the lower cavity 103 under the positive pressure in the lower cavity 103 , so that the fluid (gas or liquid) in the lower cavity 103 flows into the upper cavity 102 .

[0064] like Figure 8 As shown, when the sample flows through the valve sheet 1012 from bottom to top, the valve sheet 1012 can be pushed up, and the valve sheet 1012 will bend upward to form a gap, and the sample can flow through the valve sheet and continue to move upward. If the sample flows through the valve sheet 1012 from top to bottom, the valve sheet 1012 will cover the channel opening of the lower cavity 103 and form a seal at this position through the deformation of the valve sheet 1012, thereby preventing the upper sample from moving downward. In this embodiment, there is no specific restriction on the shape of the valve sheet, the upper cavity and the lower cavity, and the valve sheet is a soft material and is an inert material for the sample. However, it is required that the area of ​​the valve sheet 1012 is larger than the cross-sectional area of ​​the lower cavity 103, and the cross-sectional area of ​​the lower end of the upper cavity 102 is larger than the cross-sectional area of ​​the valve sheet 1012, and the cross-sectional area of ​​the valve sheet 1012 is larger than the cross-sectional area of ​​the upper end of the lower cavity 103.

[0065] In one embodiment, the one-way valve mechanism of the present application can easily separate the cavity inside the one-way valve, and can open or close the communication channel between the upper cavity and the lower cavity under pressure. Figure 4-Figure 7 As shown, by the movement of the valve plate structure in the one-way valve cavity, the valve plate structure can easily open / close the communication channel between the upper cavity and the lower cavity. Figure 6 The figure shows the situation when the lower cavity 103 in the one-way valve generates positive pressure, showing that the communication channel between the upper cavity and the lower cavity is opened, thereby achieving the flow of fluid from the lower cavity into the upper cavity; Figure 7The figure shows the situation when the lower cavity 103 in the one-way valve generates negative pressure (or no pressure), showing that the communication channel between the upper cavity and the lower cavity is closed, so that the fluid cannot flow between the upper and lower cavities. The one-way valve mechanism of the present application has a simple structure and is easy to control, and can be easily introduced into the pre-processing device of the thromboelastography instrument.

[0066] In the present application, for the one-way valve mechanism as described above, the inlet pipe 104 of the lower chamber 103 is connected to the sample outlet 32 ​​of the sample storage chamber 3 through a pipeline, and the outlet pipe 105 of the upper chamber 102 is connected to the detection chamber 8 through a pipeline, thereby allowing the sample flowing out of the sample outlet 32 ​​of the sample storage chamber 3 to be injected into the detection chamber 8 through the one-way valve mechanism.

[0067] The following describes the use of the pre-processing equipment:

[0068] The sample to be tested is connected to the sample injection device 1, and the negative pressure valve 64 is opened to generate negative pressure in the sample temporary storage chamber 3, thereby injecting the sample into the sample temporary storage chamber 3 through the sample injection device 1, the U-shaped buffer tube 5 and the goose-shaped tube 2. At this time, the one-way valve mechanism 4 is closed;

[0069] After the set amount of sample is injected, the positive pressure valve 61 is opened and the negative pressure valve 64 is closed at the same time, generating a positive pressure in the sample temporary storage chamber 3. At this time, the one-way valve mechanism 4 is opened, thereby injecting the sample in the sample temporary storage chamber 3 into the detection chamber 8 via the one-way valve mechanism 4; at the same time, the injection detector 7 can monitor the amount of sample injected into the one-way valve mechanism 4 and then injected into the detection chamber 8.

[0070] In the description of the present application, it should be noted that the terms "upper", "lower", "inside", "outside", "front", "back", "left", "right", etc. indicate directions or positional relationships based on the directions or positional relationships in the working state of the present application. They are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they should not be understood as limitations on the present application.

[0071] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "install", "connect" and "connect" should be understood in a broad sense. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0072] The present application has been described above in conjunction with preferred embodiments, but these embodiments are only exemplary and serve only as an illustration. On this basis, various replacements and improvements may be made to the present application, all of which fall within the scope of protection of the present application.

Claims

1. A pre-processing device for a thromboelastograph, characterized in that: include: A sample injection device, used to connect with the system to be injected, so that the sample in the system to be injected is transported to the pre-treatment equipment via the sample injection device; A goose-shaped tube, the goose-shaped tube is in fluid communication with the injection device, the goose-shaped tube is a microfluidic pipeline, and a U-shaped buffer tube is provided between the injection device and the goose-shaped tube; A sample temporary storage chamber, wherein the sample temporary storage chamber is provided with a sample inlet and a sample outlet, wherein the sample inlet is connected to the goose-shaped tube; the sample temporary storage chamber is also provided with one or more vents, through which positive pressure or negative pressure is generated in the sample temporary storage chamber; A one-way valve mechanism, one side of which is connected to the sample outlet of the sample temporary storage chamber, and the other side of which is connected to the detection chamber of the thromboelastograph, The pressure system includes an air pump, a positive pressure tank and a negative pressure tank, the air pump is connected to the positive pressure tank and the negative pressure tank respectively, the positive pressure tank is connected to the vent through a positive pressure valve, and the negative pressure tank is connected to the vent through a negative pressure valve; The one-way valve mechanism comprises a one-way valve cavity and a valve plate structure arranged inside the one-way valve cavity, wherein the valve plate structure comprises a fixing portion arranged on the inner wall of the one-way valve cavity, a valve plate connected to the fixing portion, and a valve plate baffle arranged on the inner wall of the one-way valve cavity, wherein the valve plate baffle supports the valve plate; The valve plate is made of soft material and will bend upward to form a gap; The upper surface end of the valve plate baffle matches the lower surface end of the valve plate.

2. The pre-processing device according to claim 1, characterized in that: The sample temporary storage chamber is communicated with the pressure system through the vent.

3. The pre-processing device according to claim 1, characterized in that: The sample temporary storage cavity is also provided with a reagent area for storing reagents near the sample outlet.

4. The pre-processing device according to claim 3, characterized in that: The sample temporary storage cavity is also provided with a reagent inlet for conveying reagents to the reagent area.

5. The pre-processing equipment according to claim 1, characterized in that: A sample inlet is arranged at the upper part of the sample temporary storage cavity, and the sample outlet is arranged at the lower part of the sample temporary storage cavity.

6. The pre-processing equipment according to claim 1, characterized in that: The pre-treatment device further comprises a sample injection detector, which is arranged between the one-way valve mechanism and the sample temporary storage chamber.

7. The pre-processing equipment according to claim 1, characterized in that: The sample injection device is a blood collection tube insertion needle.

8. The pre-processing equipment according to claim 1, characterized in that: The detection chamber is a detection cup, comprising a cup body and a cup cover, and the lower part of the cup body is connected to the one-way valve mechanism.

Images