A sample preparation device

Abstract

The present invention provides a sample preparation device, comprising: a substrate, one side surface of the substrate being a plane, and a sample storage portion being formed on the side surface of the substrate; a scraper and a base, the scraper being detachably mounted on the base, a through cavity being defined between the scraper and the base, the substrate being movably disposed in the cavity, the lower surface of the scraper being a plane and facing the side surface of the substrate; a support portion being provided on the base, the support portion being used to mount a sampling rod, the sampling rod being rotatable on the support portion about a first axis, the first axis being perpendicular to the direction of movement of the substrate, and the first axis being parallel to the side surface of the substrate. When the sample on the sample storage portion passes through the scraper, the scraper can flatten the sample, making the sample uniform, thereby avoiding sample preparation differences caused by different people preparing the sample, and the sample can be applied to a predetermined position, resulting in a uniform distribution of bacterial populations and improving the accuracy of the test results.

Description

Technical Field

[0001] The present invention relates to the field of sample detection, and in particular to a sample preparation device. Background Art

[0002] The female vaginal microbiome is a component of the human microbiome, comprised of the vaginal microbiome, endocrine regulatory system, vaginal anatomy, and local immune system. The vaginal microbiome clinical evaluation system assesses the vaginal microbiome from both a morphological and functional perspective. This includes information on the predominance, diversity, and density of the vaginal microbiome, pathogens (such as Trichomonas and fungi), Lactobacillus grading, white blood cell count, epithelial cells, and functional indicators (such as pH, sialidase, leukocyte esterase, glucuronidase, and acetylglucosaminidase). This vaginal microbiome clinical evaluation system enables accurate diagnosis of various gynecological infections, providing important laboratory diagnostic evidence and guiding effective clinical treatment.

[0003] Morphology is the most important evaluation metric in the vaginal microecological evaluation system. Currently, the common clinical procedure involves scraping secretions from the upper third of the vaginal wall with a dry cotton swab. The smear is then evenly smeared onto a clean glass slide, stained with Gram stain, and observed under an upright optical microscope. Clinical practice requires that the smear have an appropriate thickness of secretions, uniform bacterial spread, no cell stacking, uniform staining, and a clean background.

[0004] However, there are currently no standardized procedures for secretion sampling and smearing slides in clinical gynecological microecological examinations. The operator's proficiency has a great impact on the final slide preparation quality, and different people have different sample preparation results. Generally, after sampling, the sample is smeared on the slide, which can easily lead to uneven secretion smearing thickness, random smearing positions, uneven bacterial distribution, and the problem that Trichomonas in Trichomonas vaginosis is easily crushed, which can easily lead to inaccurate test results. Summary of the Invention

[0005] In view of this, the present invention provides a sample preparation device to solve the problems of existing sampling and sample smearing devices and methods, such as inconvenient sampling and smearing, uneven sample smearing, differences in sample preparation by different personnel, and inaccurate test results.

[0006] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0007] The sample preparation device according to an embodiment of the present invention includes:

[0008] a substrate, wherein one side surface of the substrate is flat, and a sample storage portion is formed on the one side surface of the substrate;

[0009] a scraper and a base, wherein the scraper is detachably mounted on the base, a through cavity is defined between the scraper and the base, the substrate is movably mounted in the cavity, and the lower surface of the scraper is flat and faces one side surface of the substrate;

[0010] The base is provided with a support portion for mounting a sampling rod. The sampling rod can rotate around a first axis on the support portion. The first axis is perpendicular to the moving direction of the substrate and parallel to a side surface of the substrate.

[0011] Wherein, the sample storage portion includes at least one groove.

[0012] Wherein, the sample storage portion includes a plurality of grooves, and the depths of at least two of the plurality of grooves are different.

[0013] Wherein, the sample storage portion includes a plurality of grooves, and the depth of at least one of the grooves changes in a step-like manner or a linear gradient.

[0014] Wherein, a hydrophilic layer is formed on the surface of the sample storage part.

[0015] Wherein, a hydrophobic layer is formed on a region of one side surface of the substrate except the sample storage portion.

[0016] Wherein, the sample storage part is transparent.

[0017] Wherein, an area on one side surface of the substrate except the sample storage portion is non-transparent.

[0018] Wherein, the lower surface of the scraper is parallel to one side surface of the substrate.

[0019] Wherein, the base is provided with a guide groove extending along the length direction of the base, the substrate is movably arranged in the guide groove along the length direction of the base, the scraper is located at one end of the guide groove, and one end of the scraper is connected to one side edge of the guide groove, and the other end of the scraper is connected to the other side edge of the guide groove.

[0020] Among them, also include:

[0021] A sampling rod is detachably arranged on the supporting portion.

[0022] Wherein, the sampling rod comprises:

[0023] a support shaft, both ends of which are rotatably disposed on the support portion;

[0024] At least one smearing portion is columnar and disposed on the support shaft, and an axis of the smearing portion is collinear with an axis of the support shaft.

[0025] There are multiple smearing parts, and the multiple smearing parts are spaced apart along the axial direction of the support shaft.

[0026] Wherein, the position of the smearing portion on the supporting shaft is adjustable.

[0027] Wherein, the applying portion is elastic.

[0028] Wherein, the support shaft is movable on the support portion along the axial direction of the support shaft.

[0029] Wherein, the support portion includes two support grooves, and both ends of the support shaft are rotatably placed in the corresponding support grooves.

[0030] The beneficial effects of the above technical solution of the present invention are as follows:

[0031] According to an embodiment of the present invention, a sample preparation device comprises a substrate having a planar side surface and a sample reservoir formed thereon; a scraper blade is detachably mounted on the base, a cavity being defined between the scraper blade and the base, and the base being movably positioned within the cavity; the lower surface of the scraper blade is planar and faces the side surface of the substrate; and a support portion is provided on the base for mounting a sampling rod. The sampling rod is rotatable on the support portion about a first axis perpendicular to the direction of movement of the substrate and parallel to the side surface of the substrate. A sample can be applied to the sample reservoir. During sample preparation, a sample can be first taken with the sampling rod, which is then mounted on the support portion. The sample on the sampling rod contacts the side surface of the substrate and is positioned corresponding to the sample reservoir. The substrate is then moved outward, and the substrate drives the sampling rod to rotate, causing the sample on the sampling rod to be applied to the sample reservoir. When the sample on the sample reservoir passes through the scraper blade, the scraper blade can flatten the sample, making the sample uniform, thereby avoiding sample preparation differences caused by different sample preparations. The sample can be applied to a predetermined location, resulting in a uniform bacterial distribution and improving the accuracy of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 A schematic structural diagram of a sample preparation device according to an embodiment of the present invention;

[0033] Figure 2 Schematic diagram of the structure of a substrate in a sample preparation device according to an embodiment of the present invention.

[0034] Reference numerals

[0035] Substrate 10; groove 11;

[0036] scraper 20;

[0037] Base 30; guide groove 31; support groove 32;

[0038] Sampling rod 40; support shaft 41; application portion 42. DETAILED DESCRIPTION

[0039] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field are within the scope of protection of the present invention.

[0040] The sample preparation device according to an embodiment of the present invention will be described in detail below with reference to the accompanying drawings.

[0041] like Figure 1 and Figure 2 As shown, the sample preparation device according to an embodiment of the present invention includes a substrate 10 , a scraper 20 and a base 30 .

[0042] Specifically, one side surface of the substrate 10 is a plane, and a sample storage portion is formed on one side surface of the substrate 10; the scraper 20 is detachably arranged on the base 30, and a through cavity is defined between the scraper 20 and the base 30, and the substrate 10 is movably arranged in the cavity, and the lower surface of the scraper 20 is a plane and faces one side surface of the substrate 10; a support portion is provided on the base 30, and the support portion is used to install a sampling rod, and the sampling rod 40 can rotate around a first axis on the support portion, and the first axis is perpendicular to the direction of movement of the substrate 10, and the first axis is parallel to one side surface of the substrate 10.

[0043] That is, the sample preparation device according to an embodiment of the present invention is mainly composed of a substrate 10, a scraper 20, and a base 30, wherein one side surface of the substrate 10 is flat, and the substrate 10 can be a glass plate or a plastic plate, or a rectangular thin sheet. A sample storage portion can be formed on one side surface of the substrate 10, and the sample storage portion can be used for coating samples. The sample storage portion can be flat or grooved. The scraper 20 is detachably mounted on the base 30, and the scraper 20 is movably mounted on the base 30. A through cavity can be defined between the scraper 20 and the base 30, and the substrate 10 is movably mounted in the cavity. The lower surface of the scraper 20 can be flat, and the lower surface of the scraper 20 faces one side surface of the substrate 10. A support portion can be provided on the base 30, and the support portion can be used to mount a sampling rod 40. The sampling rod 40 can rotate around a first axis on the support portion. The first axis can be perpendicular to the direction of movement of the substrate 10 and parallel to one side surface of the substrate 10.

[0044] During the sample preparation process, the sample can be coated on the sample storage part. The sampling rod 40 can be used to take the sample first, and then the sampling rod 40 can be installed on the support part. The sample on the sampling rod 40 contacts the surface of one side of the substrate 10. The sample on the sampling rod 40 can correspond to the position of the sample storage part, and then the substrate 10 is moved outward. During the movement, the substrate 10 drives the sampling rod 40 to rotate, so that the sample on the sampling rod 40 is coated on the sample storage part. The substrate 10 continues to move. When the sample on the sample storage part passes through the scraper 20, the scraper 20 can scrape the sample flat. The substrate 10 can be directly pulled out of the cavity to complete the sample preparation. It is simple and convenient, makes the sample uniform, avoids the sample preparation differences caused by different people preparing the sample, can be smeared at a predetermined position, the bacterial population is evenly distributed, and the accuracy of the test results is improved.

[0045] In some embodiments of the present invention, the sample storage portion may include at least one groove 11, and the sample may be applied to the groove 11. The bottom wall of the groove 11 may be transparent to facilitate light to pass through the groove 11 for sample detection. The depth of the groove 11 may be selected according to the thickness of the sample. For example, there may be three grooves 11, and the bottom wall of the groove 11 is a horizontal surface parallel to one side surface of the substrate 10, which facilitates the flattening of the sample, reduces the squeezing of the sample, and makes the thickness of the sample uniform. The sample can be confined to the groove 11 by the groove 11, which facilitates the sample to be placed in a predetermined area and prevents the flow of some thin samples.

[0046] The groove 11 may extend along the moving direction of the substrate 10 , and a plurality of grooves 11 may be spaced apart and evenly distributed along the length direction of the substrate 10 .

[0047] Optionally, the sample storage portion may include multiple grooves 11, and the depths of at least two grooves 11 among the multiple grooves 11 are different. Samples of different thicknesses can be prepared through grooves 11 of different depths. Samples of different thicknesses can be prepared simultaneously through a substrate 10, which facilitates sampling and sample preparation for detection.

[0048] Furthermore, the sample storage portion may include a plurality of grooves 11, and the depth of at least one groove 11 may vary in a stepped or linear manner, so that changes in samples of different thicknesses can be detected in one groove 11. Alternatively, the depth of the groove 11 may vary uniformly, that is, the bottom wall of the groove 11 may also be inclined.

[0049] In some embodiments of the present invention, a hydrophilic layer may be formed on the surface of the sample storage portion. Optionally, a hydrophobic layer may be formed on the area outside the sample storage portion on one side surface of the substrate 10. That is, a hydrophilic layer may be formed on the surface of the sample storage portion on one side surface of the substrate 10 to facilitate contact between the sample and the surface, avoid gaps or air between the sample and the surface, avoid the influence of gaps or air on the test results, and improve the accuracy of the test results. A hydrophobic layer may be formed on the area outside the sample storage portion on the one side surface of the substrate 10. The hydrophobic layer can prevent the sample from remaining in the area outside the sample storage portion, making it easier to clean.

[0050] In other embodiments of the present invention, the sample reservoir can be transparent. Preferably, the area on one side of the substrate 10, excluding the sample reservoir, is non-transparent. In other words, the sample reservoir can be transparent, while the area on one side of the substrate 10, excluding the sample reservoir, can be opaque. This facilitates detection of the sample in the sample reservoir, prevents the area outside the sample reservoir from being affected by light transmission, improves light contrast, and enhances the accuracy of detection results.

[0051] According to some embodiments, the lower surface of the scraper 20 is parallel to one side surface of the substrate 10 , so as to facilitate scraping the sample flat and make the sample uniform.

[0052] In an embodiment of the present invention, the base 30 may be provided with a guide groove 31 extending along the length of the base 30. The substrate 10 is movably disposed in the guide groove 31 along the length of the base 30. The scraper 20 is located at one end of the guide groove 31, with one end of the scraper 20 connected to one side edge of the guide groove 31 and the other end of the scraper 20 connected to the other side edge of the guide groove 31. The sample on the sampling rod 40 contacts one surface of the substrate 10. The substrate 10 is moved outward, and the sampling rod 40 is rotated during the movement, so that the sample on the sampling rod 40 is applied to the sample reservoir. When the sample on the sample reservoir passes through the scraper 20, the scraper 20 can flatten the sample. The substrate 10 can then be directly withdrawn from the guide groove 31 to complete the sample preparation. This ensures a uniform sample and avoids sample preparation differences caused by different people preparing the sample.

[0053] In some embodiments, the sampling device may further include a sampling rod 40, which is detachably mounted on the support portion. When sampling is required, the sampling rod 40 may be removed for sampling. After sampling is completed, the sampling rod 40 may be mounted on the support portion for subsequent completion of the sample preparation. The sampling rod 40 is replaceable, easy to use, highly efficient in smearing, and effective.

[0054] In an embodiment of the present invention, the sampling rod 40 may include a support shaft 41 and at least one application portion 42, wherein both ends of the support shaft 41 can be rotatably disposed on the support portion, the application portion 42 may be cylindrical, and the application portion 42 is disposed on the support shaft 41, and the axis of the application portion 42 is collinear with the axis of the support shaft 41 to facilitate uniform application.

[0055] Optionally, there may be multiple smearing parts 42, and the multiple smearing parts 42 may be spaced apart along the axial direction of the support shaft 41, so that multiple samples can be smeared on the substrate 10 at the same time, ensuring that the multiple samples are smeared under the same conditions, avoiding the influence caused by different smearing conditions.

[0056] Furthermore, the position of the smearing portion 42 on the supporting shaft 41 is adjustable, and the smearing portion 42 can be adjusted according to the required position of the sample during sample preparation, so that the smearing portion 42 can smear the sample at the required position.

[0057] In some embodiments, the smear portion 42 may be elastic, which can buffer the force between the smear portion 42 and the substrate 10 and prevent the trichomonas from being easily crushed.

[0058] In other embodiments, the support shaft 41 is movable on the support portion along the axial direction of the support shaft 41 , and the position of the smearing portion 42 can be adjusted so that the smearing portion 42 can smear the sample at a desired position.

[0059] In an embodiment of the present invention, the support portion may include two support grooves 32, with a support groove 32 provided on each side edge of the guide groove 31, and both ends of the support shaft 41 are rotatably placed in the corresponding support grooves 32 to facilitate stable rotation of the support shaft 41.

[0060] Unless otherwise defined, the technical or scientific terms used in the present invention shall have the ordinary meaning understood by persons having ordinary skills in the field to which the present invention belongs. The words "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Words such as "connect" or "connected" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship also changes accordingly.

[0061] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A sample preparation device, characterized in that: include: A substrate, wherein one side surface of the substrate is a plane, and a sample storage portion is formed on the one side surface of the substrate; the sample storage portion includes at least one groove, and the bottom wall of the groove is a horizontal plane parallel to the one side surface of the substrate; a scraper and a base, wherein the scraper is detachably mounted on the base, a through cavity is defined between the scraper and the base, the substrate is movably mounted in the cavity, and the lower surface of the scraper is flat and faces one side surface of the substrate; The base is provided with a support portion, the support portion is used to mount a sampling rod, the sampling rod can rotate around a first axis on the support portion, the first axis is perpendicular to the direction of movement of the substrate, and the first axis is parallel to a side surface of the substrate; When sampling, the sampling rod is removed for sampling. After the sampling is completed, the sampling rod is installed on the support part. The movement of the substrate in the cavity drives the sampling rod to rotate on the support part, and the sample on the sampling rod is applied to the sample storage part.

2. The sample preparation device according to claim 1, characterized in that: The sample storage portion includes a plurality of grooves, and at least two of the plurality of grooves have different depths.

3. The sample preparation device according to claim 1, characterized in that: The sample storage portion includes a plurality of grooves, and the depth of at least one of the grooves changes in a step-like or linear manner.

4. The sample preparation device according to claim 1, characterized in that: A hydrophilic layer is formed on the surface of the sample storage part.

5. The sample preparation device according to claim 4, characterized in that: A water-repellent layer is formed on a region of one surface of the substrate excluding the sample storage portion.

6. The sample preparation device according to claim 1, characterized in that: The sample storage part is transparent.

7. The sample preparation device according to claim 6, characterized in that: An area on one side surface of the substrate except the sample storage portion is non-transparent.

8. The sample preparation device according to claim 1, characterized in that: The lower surface of the scraper is parallel to one side surface of the substrate.

9. The sample preparation device according to claim 1, characterized in that: The base is provided with a guide groove extending along the length direction of the base, the substrate is movably arranged in the guide groove along the length direction of the base, the scraper is located at one end of the guide groove, and one end of the scraper is connected to one side edge of the guide groove, and the other end of the scraper is connected to the other side edge of the guide groove.

10. The sample preparation device according to claim 1, characterized in that: Also includes: A sampling rod is detachably arranged on the supporting portion.

11. The sample preparation device according to claim 10, characterized in that: The sampling rod comprises: a support shaft, both ends of which are rotatably disposed on the support portion; At least one smearing portion is columnar and disposed on the support shaft, and an axis of the smearing portion is collinear with an axis of the support shaft.

12. The sample preparation device according to claim 11, characterized in that: There are a plurality of the application parts, and the plurality of application parts are spaced apart from each other along the axial direction of the support shaft.

13. The sample preparation device according to claim 11, characterized in that: The position of the application portion on the support shaft is adjustable.

14. The sample preparation device according to claim 11, characterized in that: The applying portion is elastic.

15. The sample preparation device according to claim 11, characterized in that: The support shaft is movable on the support portion along an axial direction of the support shaft.

16. The sample preparation device according to claim 11, characterized in that: The support portion includes two support grooves, and both ends of the support shaft are rotatably placed in the corresponding support grooves.

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