An experimental container and a biological sample preparation device

Abstract

An embodiment of the present invention provides an experimental container, comprising: a container body; a first cover; and a processing assembly disposed on the first cover; the processing assembly comprises a guide tube that passes through the first cover and is rotatable relative to the first cover, at least one row of teeth fixed to the first cover and arranged around the guide tube, and a stirring element fixed to the guide tube; the stirring element comprises at least one cantilevered cutting blade and a structural member that cooperates with the at least one row of teeth, the structural member extending from the guide tube and covering the at least one row of teeth. In the embodiment of the present invention, tissue placed in the experimental container is cut by a rotatable cantilevered cutting blade, and tissue placed in the experimental container is ground by multiple rows of teeth fixed to the first cover and a rotatable structural member covering the same. When the cantilevered cutting blade and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding processes, so that the cells in the prepared biological sample are more fully dispersed.

Description

Technical Field

[0001] The present invention relates to the technical field of experimental instruments, and in particular to an experimental container and a biological sample preparation device. Background Art

[0002] Tissue is a cellular structure between cells and organs, composed of many morphologically similar cells and intercellular matrix. To conduct cellular research on organisms, tissue must be processed and prepared into a single-cell suspension. A single-cell suspension is a biological sample in which the connections between cells in the tissue are disrupted, releasing dispersed individual cells. The resulting single-cell suspension must exhibit sufficient cell dispersion and high activity—that is, it must fully disrupt the connections between cells while maintaining the integrity and activity of individual cells. This is the foundation of cellular research.

[0003] The aforementioned characteristics of single-cell suspensions place high demands on the structure of the containers and / or devices used to prepare them. Existing devices, when processing highly adhesive tissues such as mouse brain tissue, produce single-cell suspensions with suboptimal cell dispersion. Summary of the Invention

[0004] An embodiment of the present invention provides an experimental container, aiming to solve the problem of insufficient cell dispersion in single-cell suspensions in the prior art.

[0005] In a first aspect, an experimental container is provided, comprising:

[0006] A container body (1) having an open end (11) and a closed end (12);

[0007] a first cover (2) connected to the open end (11) of the container body (1); and

[0008] a processing assembly (3) disposed on the first cover (2);

[0009] The processing assembly (3) comprises a guide tube (31) passing through the first cover (2) and rotatable relative to the first cover (2), at least one row of teeth (32) fixed on the first cover (2) and arranged around the guide tube (31), and a stirring element (33) fixed on the guide tube (31);

[0010] The stirring element (33) comprises at least one cantilevered cutting blade (331) and a structural member (332) matched with at least one row of teeth (32); the structural member (332) extends from the guide tube (31) and covers the at least one row of teeth (32).

[0011] In a second aspect, a biological sample preparation device is provided, comprising a housing, a motor, and the experimental container as described above.

[0012] In an embodiment of the present invention, a rotatable cantilever cutting knife is used to cut tissue placed in an experimental container, and a plurality of rows of teeth fixed to a first cover and a rotatable structural member covering the same are used to grind the tissue placed in the experimental container. When the cantilever cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding processes, so that the cells in the prepared biological sample are more fully dispersed. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the following description of the embodiments with reference to the accompanying drawings, in which:

[0014] Figure 1 This is an exploded view of the experimental container provided in Example 1 of the present invention;

[0015] Figure 2 2 is a side view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present invention;

[0016] Figure 3 is a top view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present invention;

[0017] Figure 4 yes Figure 3 Schematic diagram of area B in the middle;

[0018] Figure 5 Schematic diagram of the structure of the biological sample preparation device provided in the third embodiment of the present invention. DETAILED DESCRIPTION

[0019] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar modules or modules with the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention and are not to be construed as limiting the present invention. On the contrary, the embodiments of the present invention include all variations, modifications and equivalents that fall within the spirit and scope of the appended claims.

[0020] In an embodiment of the present invention, a rotatable cantilever cutting knife is used to cut tissue placed in an experimental container, and a plurality of rows of teeth fixed to a first cover and a rotatable structural member covering the same are used to grind the tissue placed in the experimental container. When the cantilever cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding processes, so that the cells in the prepared biological sample are more fully dispersed.

[0021] Example 1

[0022] Figure 1 FIG. 1 is an exploded view of the experimental container provided in Example 1 of the present invention. Figure 1As shown, the experimental container is shaped like a test tube and includes a container body 1, a first cover 2, and a processing assembly 3. Container body 1 has an open end 11 and a closed end 12. A portion of the outer surface of open end 11 is provided with external threads that mate with the internal threads of first cover 2 to connect and secure first cover 2. Processing assembly 3 is mounted on first cover 2 and includes a guide tube 31, at least one row of teeth 32, and a stirring element 33.

[0023] The guide tube 31 extends through the center hole (not shown) of the first cover 2 and is rotatable relative to the first cover 2. In this embodiment of the present invention, three rows of teeth 32 are arranged around the guide tube 31 at different heights and fixed to the first cover 2. The stirring element 33 is fixed to the upper outer surface of the guide tube 31 and extends downward, rotatable relative to the three rows of teeth 32. The stirring element 33 includes a cantilevered cutting blade 331 and a structural member 332 that cooperates with the three rows of teeth 32. As an embodiment of the present invention, multiple cantilevered cutting blades 331 can also be provided. The structural member 332 extends from the guide tube 31 and covers the three rows of teeth 32. The distance between the corresponding surfaces of the structural member 332 and the three rows of teeth 32 is relatively short. Depending on the particle size requirements of the biological sample to be prepared, this distance is preferably approximately 100 μm when preparing a single-cell suspension and approximately 20 μm when preparing a homogenate (a biological sample that has its cell structure destroyed to release proteins and nuclei within the cells). The lower inner surface of the guide tube 31 includes a joint (not shown) for connecting to an external drive shaft. When using this experimental container for tissue processing, manually minced tissue is placed in the experimental container. The external drive shaft is connected to the joint of the guide tube 31. Rotation of the external drive shaft drives the guide tube 31 and the stirring element 33, while the container body 1, the first cover 2, and the three rows of teeth 32 fixed to the first cover 2 remain stationary. The tissue within the experimental container is cut by the cantilevered cutting blade 331 and then ground by the structural member 332, repeating the process in this cycle. The rotation speed and processing time are determined based on the tissue's cohesiveness and the desired particle size of the biological sample to be prepared.

[0024] In an embodiment of the present invention, a rotatable cantilever cutting knife is used to cut tissue placed in an experimental container, and a plurality of rows of teeth fixed to a first cover and a rotatable structural member covering the same are used to grind the tissue placed in the experimental container. When the cantilever cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding processes, so that the cells in the prepared biological sample are more fully dispersed.

[0025] Example 2

[0026] In the embodiment of the present invention, the basic structure of the experimental container is the same as that of the first embodiment, and the same components as those of the first embodiment use the same reference numerals as those of the first embodiment, including all the features described in the first embodiment, which will not be repeated here.

[0027] Figure 2 It is a side view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present invention. Figure 3 FIG is a top view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present invention. Figure 2 and Figure 3 As shown, in an embodiment of the present invention, the structural member 332 further includes a spiral baffle 3321 that forms a preset angle with the three rows of teeth 32. When the structural member 332 rotates, the tissue to be processed is pushed toward the cantilever cutting knife 331 by the spiral baffle 3321 and is cut by the cantilever cutting knife 331. The baffle 3321 is configured to be spiral, which can reduce the mechanical resistance of the tissue to be processed encountered by the structural member 332 during rotation. Preferably, at least one pressure relief port A is provided on the edge of the spiral baffle 3321. The pressure relief port A is a narrow slit that allows biological samples that have been processed into small particles to pass through, thereby further reducing the mechanical resistance of the tissue to be processed encountered by the structural member 332 during rotation.

[0028] One end of the cantilevered cutting knife 331 is fixed to the guide tube 31 and then extends outward. The vertical direction is a cutting knife with cutting edges at both ends. The end of the cutting knife extends above the three rows of teeth 32, preferably, extends to the step between the middle row of teeth and the bottom row of teeth. Figure 4 yes Figure 3 Schematic diagram of area B in the figure. Figure 4 As shown, the cross section of the cantilevered cutting blade 331 in the vertical direction is fan-shaped, with both ends having cutting edges, which can cut the tissue to be processed that it contacts during rotation. For the same cross section, the edge of the cantilevered cutting blade 331 in the vertical direction facing the middle row of teeth forms a triangle with the outer periphery of the middle row of teeth (indicated by an arc), that is, Figure 4 As shown in the figure, d1 is larger than d2, but the narrowest part still maintains a certain narrow distance, so that the cut tissue to be processed can pass through, and it can be squeezed and ground to a certain extent when passing through.

[0029] The cantilever cutting blade 331 realizes coarse cutting of the tissue to be processed, and the structural member 332 realizes fine grinding of the tissue to be processed. The coarse cutting and fine grinding are completed by two different components, so that the cells in the prepared biological sample are more fully dispersed.

[0030] As an embodiment of the present invention, when viewed from a top view, the structural member 332 and the cantilever cutting blade 331 are symmetrically distributed about the guide tube 31 and may also be set to an angle of 90 degrees, 45 degrees, etc.

[0031] In order to prevent leakage, the guide tube 31 and the three rows of teeth 32 are connected by a seal 4. Figure 1As shown, the experimental container also includes an absorption ring 5 for preventing leakage and a second cover 6. The absorption ring 5 is sleeved on the lower part of the guide tube 31 and accommodated in the second cover 6. The second cover 6 is fixed to the first cover 2 and also serves as a container for leaking liquid.

[0032] In an embodiment of the present invention, a rotatable cantilever cutting knife is used to cut tissue placed in an experimental container, and a plurality of rows of teeth fixed to a first cover and a rotatable structural member covering the same are used to grind the tissue placed in the experimental container. When the cantilever cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding processes, so that the cells in the prepared biological sample are more fully dispersed.

[0033] Example 3

[0034] Figure 5 Schematic diagram of the structure of the biological sample preparation device provided by the third embodiment of the present invention. Figure 1 and Figure 5 The biological sample preparation device includes a housing, a motor, and an experimental container as described in Example 1 or Example 2. The motor is disposed within the housing, the experimental container is placed in a notch of the housing, the container body 1 and the first cover 2 are fixed, and the motor's drive shaft is connected to the guide tube 31, driving the experimental container to rotate to prepare the biological sample.

[0035] In an embodiment of the present invention, a rotatable cantilever cutting knife is used to cut tissue placed in an experimental container, and a plurality of rows of teeth fixed to a first cover and a rotatable structural member covering the same are used to grind the tissue placed in the experimental container. When the cantilever cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding processes, so that the cells in the prepared biological sample are more fully dispersed.

[0036] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. An experimental container, characterized in that: include: A container body (1) having an open end (11) and a closed end (12); a first cover (2) connected to the open end (11) of the container body (1); and a processing assembly (3) disposed on the first cover (2); The processing assembly (3) comprises a guide tube (31) passing through the first cover (2) and rotatable relative to the first cover (2), at least one row of teeth (32) fixed on the first cover (2) and arranged around the guide tube (31), and a stirring element (33) fixed on the guide tube (31); The stirring element (33) comprises at least one cantilevered cutting blade (331) fixed at one end to the guide tube (31) and a structural member (332) matched with the at least one row of teeth (32), wherein the structural member (332) extends from the guide tube (31) and covers the at least one row of teeth (32); When the guide tube (31) and the stirring element (33) rotate, the container body (1), the first cover (2) and at least one row of teeth (32) remain stationary.

2. The experimental container according to claim 1, characterized in that For the same cross section, the edge of the at least one cantilevered cutting knife (331) facing the at least one row of teeth (32) forms a quasi-triangle with the outer periphery of the at least one row of teeth (32).

3. The experimental container according to claim 1, characterized in that The cross section of the at least one cantilevered cutting knife (331) is fan-shaped.

4. The experimental container according to claim 1, characterized in that The structural member (332) further comprises a spiral baffle (3321) forming a preset angle with the at least one row of teeth (32).

5. The experimental container according to claim 4, characterized in that At least one pressure relief port (A) is provided on the edge of the spiral baffle (3321).

6. The experimental container according to any one of claims 1 to 5, characterized in that: The structural member (332) and the at least one cantilevered cutting knife (331) are symmetrically distributed with respect to the guide tube (31).

7. The experimental container according to any one of claims 1 to 5, characterized in that: The guide tube (31) and the at least one row of teeth (32) are connected via a seal (4).

8. The experimental container according to any one of claims 1 to 5, characterized in that: The experimental container further comprises an absorption ring (5) and a second cover (6) for preventing leakage.

9. The experimental container according to any one of claims 1 to 5, characterized in that: The guide tube (31) further comprises a coupling portion adapted to be connected to an external drive shaft.

10. A biological sample preparation device, characterized in that: The invention comprises a housing, a motor and an experimental container according to any one of claims 1 to 9, wherein the motor is arranged in the housing, and the driving shaft of the motor is connected to the guide tube (31) to drive the experimental container to rotate to prepare a biological sample.

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