A biological tissue cryosection imaging device

Abstract

The present invention discloses a biological tissue low-temperature slice imaging device, comprising a defrosting mechanism, a main body mechanism, a slice mechanism and a fixing mechanism; the defrosting mechanism is located on the main body mechanism and defrosts the main body mechanism, the slice mechanism is located inside the main body mechanism and slices the biological tissue, and the fixing mechanism is also located inside the main body mechanism and fixes the biological tissue; the present invention can image the biological tissue in a low-temperature environment, a coating tube attaches a protective film to the bottom surface of a lens, so that the lens can be in a low-temperature environment, and frost forms on the outer surface of the protective film when frost forms. When imaging, the protective film and frost are shoveled to the inside of a collection box by a shovel plate, so that frost and water droplets are not generated on the lens, and the lens does not move, thereby ensuring the sealing performance of the device, preventing image deviation when the lens is imaging, and ensuring the stability and accuracy of imaging.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and more specifically to a biological tissue cryogenic slice imaging device. Background Art

[0002] Currently, when biological tissues are sliced ​​and imaged in a low-temperature environment, frost will form on the imaging lens due to the low temperature, affecting the imaging effect, causing inaccurate and difficult to distinguish data. At the same time, after slicing, due to the low temperature, the biological tissue will have strong viscosity, and the biological tissue will stick to the blade, making imaging more troublesome and affecting the imaging of the device. Summary of the invention

[0003] Therefore, the technical problem to be solved by the present invention is to provide a biological tissue cryogenic section imaging device which can prevent the imaging lens from frosting and fogging and can prevent the biological tissue from getting on the blade.

[0004] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0005] A biological tissue low-temperature section imaging device comprises a defrosting mechanism, a main body mechanism, a sectioning mechanism and a fixing mechanism; the defrosting mechanism is located on the main body mechanism and defrosts the main body mechanism, the sectioning mechanism is located inside the main body mechanism and sections the biological tissue, and the fixing mechanism is also located inside the main body mechanism and fixes the biological tissue.

[0006] The above-mentioned biological tissue low-temperature section imaging device, the defrost mechanism includes a coating box, a protective film, a film feeding plate, a sliding frame, a first screw, a first slider, a coating tube, a shovel plate, a fixed rod, a second slider, a compression spring, a fixed block, an electric telescopic rod, a defrost box, a cleaning roller, a collection box and a first cylinder; the protective film is located inside the coating box, one end of the coating box is provided with the outlet groove, one end of the protective film passes through the outlet groove at one end of the coating box, the material of the protective film is a soft transparent material, the film feeding plate is fixedly installed at the lower part of one end of the coating box, and the film feeding plate is located at the bottom of the outlet groove, the film feeding plate is placed with an inclined surface upward, and the top of the film feeding plate is in contact with the bottom surface of the protective film The cam is connected to the upper and lower surfaces of the sliding frame, and the upper and lower ends of the first sliding frame are respectively in contact with the upper and lower walls inside the sliding frame. One end of the coating tube is rotatably connected to the upper part of the first sliding frame, and one end of the protective film passes through the outlet groove and contacts with the outer wall of the coating tube. The shovel plate is located inside the two opposite sliding frames, and the shovel plate is a right-angle plate, and the top material of the shovel plate is sponge or a soft material. One end of the shovel plate is provided with an inclined surface, and the other end of the shovel plate is a straight plate. One end of the fixing rod is fixedly mounted on the outer wall of the straight plate, and the second sliding block is slidably connected to the fixing rod. The outer wall of the rod, one end of the compression spring is fixedly connected to one end of the second sliding block, and the compression spring is sleeved on the outer wall of the fixing rod, the other end of the compression spring is fixedly connected to one end of the fixing block, the fixing block is fixedly installed on the outer wall of the fixing rod, the outer wall of the right-angle plate is also fixedly installed with the electric telescopic rod, one end of the electric telescopic rod is fixedly connected to the other end of the second sliding block, the bottom of the defrost box is fixedly installed on the top of the second sliding block, the top of the defrost box is open, the cleaning roller is rotatably connected to the defrost box, and the top of the cleaning roller is located outside the top of the defrost box, the interior of the defrost box is provided with cleaning liquid, and the collection box is located at At the bottom of the second slider, the collection box can collect the residues cleaned by the defrost box and the shovel plate, and the other end of the fixing rod is fixedly connected to the output end of the first cylinder. Through the setting of the defrost mechanism, the device can image biological tissues in a low-temperature environment. The coating tube sticks the protective film to the bottom surface of the lens, so that the lens can be in a low-temperature environment. When frost forms, it forms on the outer surface of the protective film. When imaging, the protective film and frost are shoveled to the inside of the collection box through the shovel plate, so that no frost or water droplets are generated on the lens. At the same time, the lens does not move, thereby ensuring the sealing performance of the device. At the same time, it can prevent the frequent movement of the lens from causing image deviation during imaging, thereby ensuring the stability and accuracy of imaging.

[0007] The above-mentioned biological tissue low-temperature section imaging device, the main body mechanism includes a sealing box, an imaging module, an objective lens, a conveying box, a positioning plate and a conveyor belt; the imaging module is located inside the sealing box, the objective lens is located at the bottom of the imaging module, the transverse plate surface of the shovel plate is in contact with the edge of the bottom surface of the objective lens, the protective film can be attached to the surface of the objective lens, the top of the conveying box is connected to the bottom of the sealing box and the fluid is conductive, the positioning plate is fixedly connected to the inner top wall of the conveying box, and the positioning plate is vertically installed to one end of the objective lens, the conveyor belt is located inside the conveying box, and through the setting of the main body mechanism, when imaging the biological tissue, the biological tissue is conveyed to the bottom of the objective lens through the conveyor belt, so that the imaging module can perform imaging processing on the biological tissue, and at the same time, the positioning plate can accurately deliver the biological tissue to the bottom of the objective lens, so that when the imaging module images the biological tissue, it can accurately image and prevent the occurrence of imaging deviation and the like.

[0008] The above-mentioned biological tissue low-temperature slicing imaging device, the slicing mechanism includes a slicing box, a slicing knife, a decompression spring, a second cylinder, a flattening box, a flattening plate, a second lead screw, a motor, a sliding block, a fixed plate, a limiting plate and a flattening roller; the bottom of the slicing box is a conical surface, the conical surface of the bottom of the slicing box is provided with a through hole, the slicing knife is located inside the slicing box, and the bottom of the slicing knife is also a conical surface, the inner wall of the conical surface of the bottom surface of the slicing box contacts the outer wall of the slicing knife, the top of the slicing knife is fixedly connected to one end of the decompression spring, the other end of the decompression spring is fixedly connected to the output end of the second cylinder, and the top of the second cylinder is fixedly connected to the inner top wall of the conveying box; the flattening box is fixedly installed inside the conveying box, the second lead screw is rotatably connected to the inside of the flattening box, one end of the second lead screw is fixedly connected to the output end of the motor, and the sliding block is slidably connected to the On the outer wall of the second screw, one end of the paving plate is fixedly connected to the sliding block, the paving plate is placed on an inclined surface, and the other end of the paving plate can contact the slicing knife, the fixed plate is located inside the paving box, and the paving plate is rotatably connected to the inner wall of the fixed plate, the limiting plate is symmetrically installed with the paving plate, and the limiting plate can also contact the slicing knife, the paving roller is rotatably connected to the bottom of the paving plate, and the paving roller is in contact with the conveyor belt. Through the setting of the slicing mechanism, when the biological tissue is sliced, the paving plate will clean the biological tissue stuck on the slicing knife, and through the action of the paving roller, the biological tissue is flattened on the conveyor belt to prevent the slicing knife from carrying the biological tissue and preventing the biological tissue from being taken away by the slicing knife. At the same time, the limiting plate prevents the unsliced ​​biological tissue from shaking, and a decompression spring is provided on the slicing knife to reduce the radial pressure of the slicing knife and prevent damage to the slicing knife.

[0009] The above-mentioned biological tissue low-temperature section imaging device, the fixing mechanism includes a fixed base plate, a fixed box, a fixed frame, a slide groove, a third screw, a slide plate, a push plate, a retracting rod, a pressure plate, a conveying roller and a slicing plate; the fixed box is located at one end of the top of the fixed base plate, the fixed frame is located at both sides of the middle of the upper surface of the fixed base plate, and the inlet end of the fixed frame is connected with the outlet end of the fixed box, the upper surface of the fixed frame is provided with the slide groove, the third screw is rotatably connected to the inside of the slide groove, the slide plate is located at the top of the fixed frame, and the bottom of both sides of the slide plate is rotatably connected to the outer wall of the third screw, the outer wall of the slide plate is provided with the through hole, the push plate is slidably connected to the inside of the through hole, the two sides of the push plate are fixedly connected to the top of the retracting rod, and the retracting rod is located at the The top of the pressing plate is fixedly connected to the bottom of the pushing plate inside the slide, the conveying roller is located on the upper surface of the fixed bottom plate, and the conveying roller is located inside the fixed frame. The number of the conveying rollers is two or more, and the cooling module is arranged at the bottom of the conveying roller. The outlet end of the fixed frame is connected to the slicing plate, and the conveyor belt passes through the inside of the fixed bottom plate. Through the setting of the fixing mechanism, the biological tissue can be prevented from being offset when entering the device for slicing. At the same time, the operation is performed in a sealed environment to prevent external contamination. Through the setting of the pushing plate and the pressing plate, the biological tissue can be fixed, and the conveying roller can be used for conveying. At the same time, the biological tissue is cooled to prevent dissolution, so that the biological tissue can be quickly sliced ​​and imaged.

[0010] The technical solution of the present invention achieves the following beneficial technical effects:

[0011] 1. The present invention, through the setting of the defrosting mechanism, enables the device to image biological tissues in a low-temperature environment. The coating tube adheres the protective film to the bottom surface of the lens, so that the lens can be in a low-temperature environment. When frost forms, it forms on the outer surface of the protective film. When imaging, the protective film and frost are shoveled to the inside of the collection box by a shovel plate, so that no frost and water droplets are generated on the lens. At the same time, the lens does not move, thereby ensuring the sealing performance of the device. At the same time, it can prevent the frequent movement of the lens from causing image deviation during imaging, thereby ensuring the stability and accuracy of imaging.

[0012] 2. The present invention, through the setting of the main body mechanism, when imaging biological tissue, the biological tissue is conveyed to the bottom of the objective lens through the conveyor belt, so that the imaging module can perform imaging processing on the biological tissue. At the same time, the positioning plate can accurately convey the biological tissue to the bottom of the objective lens, so that when the imaging module images the biological tissue, it can accurately image and prevent the occurrence of imaging deviation and the like.

[0013] 3. The present invention, through the setting of the slicing mechanism, when the biological tissue is sliced, the paving plate will clean the biological tissue stuck on the slicing knife, and the biological tissue is flattened on the conveyor belt through the action of the paving roller, so as to prevent the biological tissue from being carried on the slicing knife and being taken away by the slicing knife. At the same time, the limiting plate prevents the unsliced ​​biological tissue from shaking. The slicing knife is provided with a decompression spring, which can reduce the radial pressure of the slicing knife and prevent the slicing knife from being damaged.

[0014] 4. The present invention, through the setting of the fixing mechanism, can prevent the biological tissue from being shifted when entering the device for slicing. At the same time, it operates in a sealed environment to prevent external contamination. Through the setting of the push plate and the pressure plate, the biological tissue can be fixed, and the conveying roller is used for conveying. At the same time, the biological tissue is cooled to prevent dissolution, so that the biological tissue can be quickly sliced ​​and imaged. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic structural diagram of the defrosting mechanism of the present invention;

[0016] Figure 2 The present invention Figure 1 The enlarged structural diagram at A in the middle;

[0017] Figure 3 A schematic diagram of the structure inside the sealing box and the transfer box of the present invention;

[0018] Figure 4 The present invention Figure 3 The enlarged structural diagram at B in the middle;

[0019] Figure 5 A schematic structural diagram of the fixing mechanism of the present invention.

[0020] The reference numerals in the figure are as follows: 100-defrosting mechanism; 200-main body mechanism; 300-slicing mechanism; 400-fixing mechanism; 101-film coating box; 102-protective film; 103-film feeding plate; 104-sliding frame; 105-first lead screw; 106-first slider; 107-film coating cylinder; 108-shovel plate; 109-fixing rod; 110-second slider; 111-compression spring; 112-fixing block; 113-electric telescopic rod; 114-defrosting box; 115-cleaning roller; 116-collecting box; 117-first cylinder; 201-sealing box; 202-imaging module; 203-object mirror; 204-transmission box; 205-positioning plate; 206-conveyor belt; 301-slicing box; 302-slicing knife; 303-decompression spring; 304-second cylinder; 305-leveling box; 306-laying plate; 307-second lead screw; 308-motor; 309-sliding block; 310-fixed plate; 311-limiting plate; 312-leveling roller; 401-fixed bottom plate; 402-fixed box; 403-fixed frame; 404-slide; 405-third lead screw; 406-slide plate; 407-push plate; 408-retraction rod; 409-pressing plate; 410-transmission roller; 411-slicing plate. DETAILED DESCRIPTION

[0021] The present embodiment provides a biological tissue low-temperature section imaging device, comprising a defrost mechanism 100, a main body mechanism 200, a sectioning mechanism 300 and a fixing mechanism 400; the defrost mechanism 100 is located on the main body mechanism 200 and defrosts the main body mechanism 200, the sectioning mechanism 300 is located inside the main body mechanism 200 and sections the biological tissue, and the fixing mechanism 400 is also located inside the main body mechanism 200 and fixes the biological tissue.

[0022] like Figure 1-3As shown, the defrosting mechanism 100 includes a coating box 101, a protective film 102, a film feeding plate 103, a sliding frame 104, a first screw 105, a first slider 106, a coating cylinder 107, a shovel plate 108, a fixing rod 109, a second slider 110, a compression spring 111, a fixing block 112, an electric telescopic rod 113, a defrosting box 114, a cleaning roller 115, a collecting box 116 and a first cylinder 117; the protective film 102 is located inside the coating box 101, an outlet groove is provided at one end of the coating box 101, one end of the protective film 102 passes through the outlet groove at one end of the coating box 101, and the material of the protective film 102 is a soft transparent material , the film feeding plate 103 is fixedly installed at the lower part of one end of the film coating box 101, and the film feeding plate 103 is located at the bottom of the outlet slot, the film feeding plate 103 is placed with the inclined surface upward, the top of the film feeding plate 103 contacts the bottom surface of the protective film 102, the first lead screw 105 is rotatably connected to the inside of the sliding frame 104, the lower part of the first slider 106 is slidably connected to the outer wall of the first lead screw 105, and the upper and lower ends of the first slider 106 are respectively in contact with the upper and lower walls inside the sliding frame 104, one end of the coating tube 107 is rotatably connected to the upper part of the inside of the first slider 106, and the end of the protective film 102 passing through the outlet slot contacts the outer wall of the coating tube 107;The shovel plate 108 is located inside the two opposite sliding frames 104. The shovel plate 108 is a right-angle plate, and the top material of the shovel plate 108 is sponge or a soft material. One end of the shovel plate 108 is provided with an inclined surface, and the other end of the shovel plate 108 is a straight plate. One end of the fixing rod 109 is fixedly installed on the outer wall of the straight plate, and the second sliding block 110 is slidably connected to the outer wall of the fixing rod 109, one end of the compression spring 111 is fixedly connected to one end of the second sliding block 110, and the compression spring 111 is sleeved on the outer wall of the fixing rod 109, and the other end of the compression spring 111 is fixedly connected to one end of the fixing block 112, and the fixing block 112 is fixedly installed on the outer wall of the fixing rod 109. An electric telescopic rod 113 is also fixedly installed on the outer wall of the right-angle plate, and one end of the electric telescopic rod 113 is fixedly connected to the other end of the second sliding block 110. The bottom of the defrost box 114 is fixedly installed on the top of the second sliding block 110, and the top of the defrost box 114 is open. The cleaning roller 115 is rotatably connected to the defrost The defrosting box 114 is provided with cleaning liquid inside the defrosting box 114, and the top of the cleaning roller 115 is located outside the top of the defrosting box 114. The defrosting box 114 is provided with cleaning liquid inside. The collecting box 116 is located at the bottom of the second slider 110. The collecting box 116 can collect the residues cleaned by the defrosting box 114 and the shovel plate 108. The other end of the fixing rod 109 is fixedly connected to the output end of the first cylinder 117. Through the setting of the defrosting mechanism 100, the device can image biological tissues in a low-temperature environment. The film coating cylinder 107 attaches the protective film 102 to the bottom surface of the lens, so that the lens can be in a low-temperature environment. When frost forms, it forms on the outer surface of the protective film 102. When imaging, the protective film 102 and the frost are shoveled to the inside of the collecting box 116 by the shovel plate 108, so that no frost or water droplets are generated on the lens. At the same time, the lens does not move, thereby ensuring the sealing performance of the device, and preventing the frequent movement of the lens from causing image deviation during imaging, thereby ensuring the stability and accuracy of imaging. ;

[0023] like Figure 3As shown, the main body mechanism 200 includes a sealed box 201, an imaging module 202, an objective lens 203, a transfer box 204, a positioning plate 205 and a conveyor belt 206; the imaging module 202 is located inside the sealed box 201, the objective lens 203 is located at the bottom of the imaging module 202, the lateral plate surface of the shovel plate 108 is in contact with the edge of the bottom surface of the objective lens 203, the protective film 102 can be attached to the surface of the objective lens 203, the top of the transfer box 204 is connected to the bottom of the sealed box 201 and the fluid is conductive, and the positioning plate 205 is fixed to the inner top wall of the transfer box 204. The conveyor belt 206 is located inside the conveyor box 204. Through the arrangement of the main body mechanism 200, when imaging biological tissue, the biological tissue is conveyed to the bottom of the objective lens 203 through the conveyor belt 206, so that the imaging module 202 can perform imaging processing on the biological tissue. At the same time, the positioning plate 204 can accurately deliver the biological tissue to the bottom of the objective lens 203, so that when the imaging module 202 images the biological tissue, it can accurately image and prevent the occurrence of imaging deviation and the like.

[0024] like Figure 3-4As shown, the slicing mechanism 300 includes a slicing box 301, a slicing knife 302, a decompression spring 303, a second cylinder 304, a flattening box 305, a flattening plate 306, a second lead screw 307, a motor 308, a sliding block 309, a fixing plate 310, a limiting plate 311 and a flattening roller 312; the bottom of the slicing box 301 is a conical surface, and the conical surface of the bottom of the slicing box 301 is provided with a through hole, the slicing knife 302 is located inside the slicing box 301, and the bottom of the slicing knife 302 is also a conical surface, and the inner wall of the conical surface of the bottom of the slicing box 301 The second screw 307 is rotatably connected to the inside of the flattening box 305, and one end of the second screw 307 is fixedly connected to the output end of the motor 308. The sliding block 309 is slidably connected to the second screw 305. 7, one end of the paving plate 306 is fixedly connected to the sliding block 309, the paving plate 306 is placed on an inclined surface, and the other end of the paving plate 306 can contact the slicing knife 302, the fixed plate 310 is located inside the paving box 305, and the paving plate 306 is rotatably connected to the inner wall of the fixed plate 310, the limiting plate 311 is symmetrically installed with the paving plate 306, and the limiting plate 311 can also contact the slicing knife 302, the paving roller 312 is rotatably connected to the bottom of the paving plate 306, and the paving roller 312 is in contact with the conveyor belt 206, Through the setting of the slicing mechanism 300, when the biological tissue is sliced, the paving plate 306 clears the biological tissue stuck on the slicing knife 302, and the biological tissue is flattened on the conveyor belt 206 through the action of the flattening roller 323, so as to prevent the slicing knife 302 from carrying the biological tissue and preventing the biological tissue from being taken away by the slicing knife. At the same time, the limiting plate 310 prevents the unsliced ​​biological tissue from shaking. The decompression spring 303 is arranged on the slicing knife 302, which can reduce the radial pressure of the slicing knife 302 and prevent the slicing knife 302 from being damaged.

[0025] like Figure 5As shown, the fixing mechanism 400 includes a fixed base plate 401, a fixed box 402, a fixed frame 403, a slide groove 404, a third lead screw 405, a slide plate 406, a push plate 407, a retracting rod 408, a pressing plate 409, a conveying roller 410 and a slicing plate 411; the fixed box 402 is located at one end of the top of the fixed base plate 401, the fixed frame 403 is located on both sides of the middle of the upper surface of the fixed base plate 401, and the inlet end of the fixed frame 403 is connected with the outlet end of the fixed box 402, the upper surface of the fixed frame 403 is provided with a slide groove 404, the third lead screw 405 is rotatably connected to the inside of the slide groove 404, the slide plate 406 is located at the top of the fixed frame 403, and the bottom of both sides of the slide plate 406 is rotatably connected to the outer wall of the third lead screw 405, the outer wall of the slide plate 406 is provided with a through hole, the push plate 407 is slidably connected to the inside of the through hole, the two sides of the push plate 407 are fixedly connected to the top of the retracting rod 408, and the retracting rod 410 is connected to the retracting rod 411. 08 is located inside the slide plate 406, the top of the pressure plate 409 is fixedly connected to the bottom of the push plate 407, the conveying roller 410 is located on the upper surface of the fixed bottom plate 401, and the conveying roller 410 is located inside the fixed frame 403. The number of conveying rollers 410 is two or more, and a cooling module is arranged at the bottom of the conveying roller 410. The outlet end of the fixed frame 403 is connected to the slicing plate 411, and the conveyor belt 206 passes through the inside of the fixed bottom plate 401. Through the setting of the fixing mechanism 400, the biological tissue can be prevented from being offset when entering the device for slicing. At the same time, the operation is performed in a sealed environment to prevent external contamination. Through the setting of the push plate 407 and the pressure plate 409, the biological tissue can be fixed, and the conveying roller 410 is used for conveying. At the same time, the biological tissue is cooled to prevent dissolution, so that the biological tissue can be quickly sliced ​​and imaged.

[0026] Working principle: The protective film 102 is sent to the outer wall of the film coating tube 107 by the film feeding plate 103. At this time, the first lead screw 105 is started to rotate the first slider 106, thereby driving the film coating tube 107 to rotate, so that the protective film 102 is attached to the outer wall of the objective lens 203 to prevent frost on the objective lens 203. When imaging biological tissues, the electric telescopic rod 113 is retracted first, and the fixed block 112 is displaced under the action of the compression spring 111, thereby driving the defrosting box 114 and the collection box 116 to be displaced, so that the collection box 116 is located at the bottom of the shovel plate 108. The defrosting box 114 is in contact with one end of the shovel plate 108. At this time, the first cylinder 117 is started, driving the shovel plate 108 to scrape the protective film 102 on the objective lens 203, so that the frost on the objective lens 203 is scraped off. The collecting box 116 collects the protective film 102 and the frost. At the same time, the cleaning roller 115 wipes the objective lens 203 again to prevent it from falling and affecting the imaging. Because the shovel plate 108 is in contact with the objective lens 203 when the protective film 102 is applied, the edge of the protective film 102 is in contact with the bottom surface of the shovel plate 108. When the biological tissue is cut, First, the biological tissue passes through the fixed box 402, and then is sent out through the conveying roller 410, and is further cooled at the same time. At this time, the contraction rod 408 contracts to drive the push plate 407 and the pressure plate 409 to move downward, so that the pressure plate 409 presses the biological tissue. The push plate 407 is located at the rear end of the biological tissue. Under the action of the third lead screw 405, the slide plate 406 is driven to move inside the slide groove 404, thereby pushing the biological tissue to move and playing a fixing role at the same time, so that the biological tissue is conveyed to the slicing plate 411. At this time, the second cylinder 304 is started to drive the slicing knife 30 2 moves downward to slice the biological tissue, and the decompression spring 303 reduces the radial force of the slicer 302. At this time, in order to prevent the biological tissue from sticking to the slicer 302, the limiting plate 310 limits the biological tissue, and the paving plate 306 blocks the sliced ​​biological tissue. Then, the motor 308 drives the second lead screw 307 to rotate, so that the paving plate 306 drives the paving roller 312 to move, so that the paving roller 312 spreads the sliced ​​biological tissue on the conveyor belt 206. Through the action of the positioning plate 205, the biological tissue can be accurately imaged.

[0027] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the claims of this patent application.

Claims

1. A biological tissue cryosection imaging device, characterized in that: It comprises a defrosting mechanism (100), a main body mechanism (200), a slicing mechanism (300) and a fixing mechanism (400); The defrosting mechanism (100) is located on the main body mechanism (200) and defrosts the main body mechanism (200); the slicing mechanism (300) is located inside the main body mechanism (200) and slices the biological tissue; the fixing mechanism (400) is also located inside the main body mechanism (200) and fixes the biological tissue; The defrosting mechanism (100) comprises a film coating box (101), a protective film (102), a film feeding plate (103), a sliding frame (104), a first screw (105), a first slider (106), a film coating cylinder (107), a shovel plate (108), a fixed rod (109), a second slider (110), a compression spring (111), a fixed block (112), an electric telescopic rod (113), a defrosting box (114), a cleaning roller (115), a collecting box (116) and a first cylinder (117); The protective film (102) is located inside the film coating box (101); an outlet slot is provided at one end of the film coating box (101); one end of the protective film (102) passes through the outlet slot at one end of the film coating box (101); the material of the protective film (102) is a soft transparent material; the film delivery plate (103) is fixedly mounted at the lower part of one end of the film coating box (101); and the film delivery plate (103) is located at the bottom of the outlet slot; the film delivery plate (103) is placed with an inclined surface upward; the top of the film delivery plate (103) is aligned with the protective film The first lead screw (105) is rotatably connected to the inside of the sliding frame (104), the lower part of the first slider (106) is slidably connected to the outer wall of the first lead screw (105), and the upper and lower ends of the first slider (106) are respectively in contact with the upper and lower walls inside the sliding frame (104), one end of the coating tube (107) is rotatably connected to the upper part of the inside of the first slider (106), and one end of the protective film (102) passing through the outlet slot is in contact with the outer wall of the coating tube (107); The shovel plate (108) is located inside the two opposite sliding frames (104); the shovel plate (108) is a right-angle plate, and the top material of the shovel plate (108) is sponge or a soft material; one end of the shovel plate (108) is provided with an inclined surface, and the other end of the shovel plate (108) is a straight plate; one end of the fixing rod (109) is fixedly mounted on the outer wall of the straight plate; the second sliding block (110) is slidably connected to the outer wall of the fixing rod (109); one end of the compression spring (111) is fixedly connected to one end of the second sliding block (110), and the compression spring (111) is sleeved on the outer wall of the fixing rod (109); the other end of the compression spring (111) is fixedly connected to one end of the fixing block (112), and the fixing block (112) is fixedly mounted on the outer wall of the fixing rod (109); the straight The electric telescopic rod (113) is also fixedly mounted on the outer wall of the angle plate, one end of the electric telescopic rod (113) is fixedly connected to the other end of the second slider (110), the bottom of the defrost box (114) is fixedly mounted on the top of the second slider (110), the top of the defrost box (114) is open, the cleaning roller (115) is rotatably connected to the defrost box (114), and the top of the cleaning roller (115) is located outside the top of the defrost box (114), cleaning liquid is arranged inside the defrost box (114), the collection box (116) is located at the bottom of the second slider (110), the collection box (116) can collect the residues cleaned by the defrost box (114) and the shovel plate (108), and the other end of the fixed rod (109) is fixedly connected to the output end of the first cylinder (117); The slicing mechanism (300) comprises a slicing box (301), a slicing knife (302), a decompression spring (303), a second cylinder (304), a flattening box (305), a flattening plate (306), a second lead screw (307), a motor (308), a sliding block (309), a fixing plate (310), a limiting plate (311) and a flattening roller (312); The bottom of the slicing box (301) is a conical surface, and a through hole is formed on the conical surface of the bottom of the slicing box (301). The slicing knife (302) is located inside the slicing box (301), and the bottom of the slicing knife (302) is also a conical surface. The inner wall of the conical surface of the bottom of the slicing box (301) contacts the outer wall of the slicing knife (302). The top of the slicing knife (302) is fixedly connected to one end of the decompression spring (303), and the other end of the decompression spring (303) is fixedly connected to the output end of the second cylinder (304). The top of the second cylinder (304) is fixedly connected to the inner top wall of the conveying box (204). The paving box (305) is fixedly installed inside the conveying box (204), the second lead screw (307) is rotatably connected inside the paving box (305), one end of the second lead screw (307) is fixedly connected to the output end of the motor (308), the sliding block (309) is slidably connected to the outer wall of the second lead screw (307), one end of the paving plate (306) is fixedly connected to the sliding block (309), the paving plate (306) is placed on an inclined surface, and the paving plate (306) The other end can be in contact with the slicing knife (302), the fixed plate (310) is located inside the flattening box (305), and the flattening plate (306) is rotatably connected to the inner wall of the fixed plate (310), the limiting plate (311) is symmetrically installed with the flattening plate (306), and the limiting plate (311) can also be in contact with the slicing knife (302), the flattening roller (312) is rotatably connected to the bottom of the flattening plate (306), and the flattening roller (312) is in contact with the conveyor belt (206).

2. A biological tissue cryosection imaging device according to claim 1, characterized in that: The main body mechanism (200) comprises a sealing box (201), an imaging module (202), an objective lens (203), a conveying box (204), a positioning plate (205) and a conveying belt (206); The imaging module (202) is located inside the sealed box (201), the objective lens (203) is located at the bottom of the imaging module (202), the lateral plate surface of the shovel plate (108) is in contact with the edge of the bottom surface of the objective lens (203), the protective film (102) can be attached to the surface of the objective lens (203), the top of the conveying box (204) is connected to the bottom of the sealed box (201) and the fluid is conductive, the positioning plate (205) is fixedly connected to the inner top wall of the conveying box (204), and the positioning plate (205) is vertically installed with one end of the objective lens (203), and the conveyor belt (206) is located inside the conveying box (204).

3. The biological tissue cryosection imaging device according to claim 1, characterized in that: The fixing mechanism (400) comprises a fixing bottom plate (401), a fixing box (402), a fixing frame (403), a sliding groove (404), a third lead screw (405), a sliding plate (406), a pushing plate (407), a retracting rod (408), a pressing plate (409), a conveying roller (410) and a slicing plate (411); The fixed box (402) is located at one end of the top of the fixed base plate (401), the fixed frame (403) is located on both sides of the middle of the upper surface of the fixed base plate (401), and the inlet end of the fixed frame (403) is connected to the outlet end of the fixed box (402), the upper surface of the fixed frame (403) is provided with the sliding groove (404), the third lead screw (405) is rotatably connected to the inside of the sliding groove (404), the slide plate (406) is located at the top of the fixed frame (403), and the bottom of both sides of the slide plate (406) is rotatably connected to the outer wall of the third lead screw (405), the outer wall of the slide plate (406) is provided with the through hole, and the push plate (407) is slidably connected Inside the through hole, both sides of the push plate (407) are fixedly connected to the top of the retracting rod (408), the retracting rod (408) is located inside the slide plate (406), the top of the pressure plate (409) is fixedly connected to the bottom end of the push plate (407), the conveying roller (410) is located on the upper surface of the fixed bottom plate (401), and the conveying roller (410) is located inside the fixed frame (403), the number of the conveying rollers (410) is two or more, a cooling module is provided at the bottom of the conveying roller (410), the outlet end of the fixed frame (403) is connected to the slicing plate (411), and the conveyor belt (206) passes through the inside of the fixed bottom plate (401).

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