Sample testing institutions and molecular testing equipment

Abstract

The present application relates to the field of molecular detection technology, and in particular to a sample detection mechanism and a molecular detection device. The sample detection mechanism includes a heating device, an optical detection device and a transfer member; the heating device is formed with a heating part, and the heating part is used to cyclically heat the sample to be detected; the transfer member is used to transfer the sample to be detected between the heating part and the optical detection device; the optical detection device is used to perform optical detection on the sample to be detected that has completed the thermal cycle; the optical detection device includes an optical groove, a light source assembly and an imaging assembly; the optical groove is provided with an inlet, and the sample to be detected can enter the optical groove from the inlet; one or more reflectors are arranged inside the optical groove to reflect the light in the optical groove; the light emitted by the light source assembly can be reflected by the reflector in the optical groove to different positions of the sample to be detected. The sample detection mechanism provided by the present application can perform optical detection on the sample, improve the detection efficiency, and save labor costs.

Description

Technical Field

[0001] The present application relates to the technical field of molecular detection, and in particular to a sample detection mechanism and a molecular detection device. Background Art

[0002] The basis of molecular testing is to analyze the genes and their expression products in the tissue cells, hair, anticoagulant blood or dried blood stains of the person being tested, as well as formaldehyde-fixed and paraffin-embedded tissues, to complete nucleic acid testing at the molecular level.

[0003] Due to the complexity of the existing molecular testing technology, the testing process is not only cumbersome, time-consuming and labor-intensive, but also takes too long. Summary of the invention

[0004] The purpose of this application is to provide a sample detection mechanism and molecular detection equipment to improve detection efficiency and save manpower.

[0005] The present application provides a sample detection mechanism, including a heating device, an optical detection device and a transfer member;

[0006] The heating device is formed with a heating part, and the heating part is used for cyclically heating the sample to be detected;

[0007] The transfer member is used to transfer the sample to be detected between the heating part and the optical detection device;

[0008] The optical detection device is used to perform optical detection on the sample to be detected that has completed the thermal cycle;

[0009] The optical detection device includes an optical slot, a light source assembly and an imaging assembly; the optical slot is provided with an insertion entrance, and the sample to be detected can enter the optical slot from the insertion entrance; one or more reflectors are arranged inside the optical slot, and the reflectors are used to reflect the light in the optical slot; the light emitted by the light source assembly can be reflected by the reflectors in the optical slot to different positions of the sample to be detected.

[0010] In the above technical solution, further, the optical groove has a first side wall, a first light-transmitting portion is formed on the first side wall, and the light in the optical groove can pass through the first light-transmitting portion and enter the imaging component.

[0011] In the above technical solution, further, the optical slot has a second side wall, the second side wall is provided with a second light-transmitting portion, the light source assembly can move relative to the optical slot, the light source assembly includes a plurality of illumination light sources arranged in a row, any of the illumination light sources can move to the second light-transmitting portion, and the plurality of illumination light sources are used to emit light of different wavelengths;

[0012] The imaging component can capture imaging images of the sample to be detected under light of different wavelengths.

[0013] In the above technical solution, further, the optical groove has a second side wall, and the light source assembly is installed on the second side wall;

[0014] The light source assembly includes a plurality of illumination light sources, and the plurality of illumination light sources are used to emit light of different wavelengths; the imaging assembly can capture imaging images of the sample to be detected under light of different wavelengths.

[0015] In the above technical solution, further, the optical groove has a second side wall, the number of the second side walls is two, the two second side walls are arranged opposite to each other in the optical groove, and the light source assembly is installed on both the second side walls;

[0016] The light source assembly includes a plurality of illumination light sources, and the plurality of illumination light sources are used to emit light of different wavelengths; the imaging assembly can capture imaging images of the sample to be detected under light of different wavelengths.

[0017] In the above technical solution, further, when there are multiple reflectors in the optical groove, the reflector is provided on at least the side wall of the optical groove opposite to the light source assembly.

[0018] In the above technical solution, further, the imaging assembly includes a filter and a shooting device; the shooting device is arranged opposite to the first light-transmitting portion;

[0019] The optical filter is arranged between the shooting device and the optical slot, and the optical filter can move relative to the optical slot; the optical filter includes a plurality of optical filters arranged in a row, and any of the optical filters can move to the first light-transmitting portion;

[0020] The optical filters correspond to the illumination light sources one by one, and the light rays of different wavelengths emitted by the illumination light sources can respectively pass through the corresponding optical filters and enter the shooting component.

[0021] In the above technical solution, further, the transfer component is provided to include a motion device, a mechanical arm and a shock absorbing component;

[0022] The motion device is connected to the robotic arm to drive the robotic arm to move; the robotic arm is used to carry the sample to be detected; the robotic arm is provided with the shock absorbing component, and when the transfer component transports the sample to be detected, the shock absorbing component is used to dampen the movement of the sample to be detected.

[0023] The present application also provides a molecular detection device, including a sample processing mechanism, a sample transfer mechanism and a sample detection mechanism;

[0024] The sample processing mechanism is capable of carrying samples to be tested;

[0025] The sample transfer mechanism is used to transport the sample to be tested to the sample testing mechanism;

[0026] The sample detection mechanism is used to perform nucleic acid detection on the sample to be detected.

[0027] In the above technical solution, further, the sample processing mechanism includes a carrier box and a pipetting valve assembly;

[0028] The carrying box is provided with a plurality of reagent chambers, the pipetting valve assembly is movably connected to the carrying box, and the pipetting valve assembly can move so as to communicate with the corresponding reagent chambers.

[0029] In the above technical solution, further, the pipetting valve assembly includes at least one pipetting valve;

[0030] The pipetting valve comprises a valve body and a valve stem connected to each other; the valve body is rotatably connected to the carrier box, and the valve body can be respectively connected to the plurality of reagent chambers; the valve stem can reciprocate relative to the valve body along a first direction to change the pressure in the valve body, so that the valve body absorbs or discharges the detection reagent and the sample to be detected;

[0031] When there are multiple pipetting valves, the multiple pipetting valves can be connected to each other, so that the detection reagent and the sample to be detected can be transferred between the multiple pipetting valves.

[0032] In the above technical solution, further, the sample processing mechanism also includes a pipetting drive mechanism, and the pipetting drive mechanism includes a rotating mechanism and a push-pull mechanism arranged corresponding to the pipetting valve; the rotating mechanism is used to drive the valve body of the pipetting valve to rotate, and the push-pull mechanism is used to push and pull the valve stem reciprocatingly along the first direction.

[0033] In the above technical solution, further, the rotating mechanism includes a rotating driving device, a connecting shaft, and a main gear and a sub-gear that are meshed and connected; the output end of the rotating driving device is connected to the main gear, one end of the connecting shaft is connected to the sub-gear, and the other end of the connecting shaft is detachably connected to the valve body to drive the valve body to rotate;

[0034] The push-pull mechanism includes a push-pull driving device and a first electric claw for clamping the valve stem. The output end of the push-pull driving device is connected to the first electric claw to drive the first electric claw to approach or move away from the valve stem.

[0035] In the above technical solution, further, the sample processing mechanism also includes a frame, and the carrying box and the pipetting drive mechanism are both installed on the frame;

[0036] The frame is also provided with a guide rail and a carrier box driving device; the carrier box is slidably connected to the guide rail via a slide, and the driving end of the carrier box driving device is connected to the slide, and the carrier box can reciprocate along a second direction to approach or move away from the pipetting drive mechanism; the second direction is perpendicular to the first direction.

[0037] In the above technical solution, further, the sample processing mechanism also includes an auxiliary mechanism, and the auxiliary mechanism is located on a side of the carrier box away from the pipetting drive mechanism;

[0038] The auxiliary mechanism includes an ultrasonic generator and a magnetic attraction device; the ultrasonic generator and the magnetic attraction device can move relative to the carrying box on a plane perpendicular to the height direction of the carrying box to successively approach or move away from the carrying box;

[0039] The ultrasonic generator and the magnetic device can simultaneously reciprocate along the height direction of the carrying box, so that the transducer of the ultrasonic generator fits with the bottom wall of the carrying box, and the magnetic device fits with the side wall of the carrying box;

[0040] The ultrasonic generator is provided with a heating element, and the heating element is used to fit with the bottom wall of the carrying box to provide heat.

[0041] In the above technical solution, further, the carrying box is installed with a container to be detected, and the pipetting valve assembly can be connected to the container to be detected to inject the sample to be detected into the container to be detected;

[0042] The sample transfer mechanism includes a second electric claw, a flip mechanism, a translation mechanism and a lifting mechanism;

[0043] The second electric clamp is used to clamp the container to be tested, and the flipping mechanism is connected to the second electric clamp to rotate the second electric clamp, thereby flipping the test container and moving it to the sample testing mechanism;

[0044] The carrying box is provided with a mounting frame and a sealing cover plate, the mounting frame and the sealing cover plate are both detachably connected to the carrying box, the detection container is mounted on the mounting frame, and the sealing cover plate can seal the opening of the detection container;

[0045] The lifting mechanism is connected to the flipping mechanism and is used to raise or lower the detection container; the translation mechanism is connected to the lifting mechanism and can move the detection container from the mounting frame to the sealing cover plate.

[0046] In the above technical solution, further, the sample transfer mechanism includes a support frame;

[0047] The flipping mechanism is installed on the supporting frame, and the driving end of the flipping mechanism is connected to the second electric claw;

[0048] The lifting mechanism includes a lifting drive device and a first connecting rod, and the lifting drive device is connected to the support frame through the first connecting rod; the translation mechanism includes a translation drive device and a second connecting rod, and the translation mechanism is connected to the lifting drive device through the second connecting rod.

[0049] In the above technical solution, further, the sample detection mechanism is the sample detection mechanism described in the above solution.

[0050] Compared with the prior art, the beneficial effects of this application are:

[0051] The sample detection mechanism provided in the present application can perform optical detection on samples, improve detection efficiency, and save labor costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] In order to more clearly illustrate the specific implementation methods of the present application or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0053] Figure 1 A schematic diagram of the structure of the molecular detection device provided in this application;

[0054] Figure 2 A schematic diagram of the structure of the sample processing mechanism provided for this application;

[0055] Figure 3 A schematic diagram of the assembly structure of the carrier box and the pipetting valve assembly provided in this application;

[0056] Figure 4 A schematic diagram of the bottom structure of the carrier box provided in this application;

[0057] Figure 5 A schematic diagram of the assembly structure of the carrier box and the chip board provided in this application;

[0058] Figure 6 A schematic diagram of the structure of the liquid transfer drive mechanism provided in this application;

[0059] Figure 7 A schematic diagram of the structure of the rotating mechanism provided in this application;

[0060] Figure 8 A schematic diagram of the structure of the auxiliary mechanism provided for this application;

[0061] Fig. 9 A schematic diagram of the structure of the sample transfer mechanism provided for this application;

[0062] Fig.10 A schematic diagram of the structure of the sample testing mechanism provided for this application;

[0063] Fig.11 A schematic diagram of the structure of the optical detection device provided in this application at a first viewing angle;

[0064] Fig.12 A schematic diagram of the structure of the optical detection device provided by the present application at a second viewing angle;

[0065] Fig.13 A schematic diagram of the structure of the transfer component provided in this application;

[0066] Fig.14 This is another schematic diagram of the structure of the optical detection device provided in the present application.

[0067] In the figure:

[0068] 1-sample processing mechanism; 11-carrying box; 111-container to be tested; 112-mounting frame; 113-sealing cover plate; 114-chip board; 115-redissolving chamber; 116-first flow channel; 117-second flow channel; 118-third flow channel; 119-fourth flow channel; 121-first liquid transfer valve; 122-second liquid transfer valve; 123-third liquid transfer valve; 13-liquid transfer drive mechanism; 131-rotation mechanism; 1311-rotation drive device; 1312-main Gear; 1313-secondary gear; 1314-connecting shaft; 132-push-pull mechanism; 1321-push-pull driving device; 1322-first electric claw; 14-frame; 141-first mounting plate; 142-second mounting plate; 143-mounting platform; 144-spacing adjustment mechanism; 15-auxiliary mechanism; 151-ultrasonic generator; 152-magnetic device; 153-heating element; 154-turntable component; 155-cam; 156-cam driving device;

[0069] 2-sample transfer mechanism; 21-second electric claw; 22-flipping mechanism; 23-translation mechanism; 24-lifting mechanism; 25-support frame; 26-guide rod;

[0070] 3-sample detection mechanism; 31-heating device; 311-heating tank; 32-optical detection device; 321-optical tank; 3211-light focusing element; 322-light source assembly; 323-imaging assembly; 3231-filter; 3232-shooting component; 33-transfer component; 331-first guide rail; 332-second guide rail; 333-moving drive device; 334-mechanical arm. DETAILED DESCRIPTION

[0071] The following specific embodiments are provided to help the reader obtain a comprehensive understanding of the methods, devices and / or systems described herein. However, after understanding the disclosure of the present application, various changes, modifications and equivalents of the methods, devices and / or systems described herein will be apparent. For example, the order of operations described herein is merely an example, and is not limited to the order set forth herein, but in addition to the operations that must occur in a particular order, changes that will be apparent after understanding the disclosure of the present application may be made. In addition, in order to improve clarity and brevity, descriptions of features known in the art may be omitted.

[0072] The features described herein may be implemented in different forms and should not be interpreted as being limited to the examples described herein. Rather, the examples described herein have been provided only to illustrate some of the many possible ways of implementing the methods, devices and / or systems described herein that will be apparent after understanding the disclosure of the present application.

[0073] Throughout the specification, when an element (such as a layer, a region, or a substrate) is described as being “on”, “connected to”, “bound to”, “over”, or “covering” another element, it may be directly “on”, “connected to”, “bound to”, “over”, or “covering” another element, or one or more other elements may be present between them. In contrast, when an element is described as being “directly on”, “directly connected to”, “directly bound to”, “directly over”, or “directly covering” another element, there may be no other elements present between them.

[0074] As used herein, the term "and / or" includes any one of the associated listed items and any combination of any two or more items.

[0075] Although terms such as "first," "second," and "third" may be used herein to describe various members, components, regions, layers, or portions, these members, components, regions, layers, or portions are not limited by these terms. Rather, these terms are only used to distinguish one member, component, region, layer, or portion from another member, component, region, layer, or portion. Therefore, without departing from the teachings of the examples described herein, the first member, component, region, layer, or portion referred to may also be referred to as the second member, component, region, layer, or portion.

[0076] For ease of description, spatial relational terms such as "above", "upper", "below", and "lower" may be used herein to describe the relationship of one element to another element as shown in the accompanying drawings. Such spatial relational terms are intended to include different orientations of the device in use or operation in addition to the orientation depicted in the accompanying drawings. For example, if the device in the accompanying drawings is turned over, an element described as being "above" or "upper" relative to another element will subsequently be "below" or "lower" relative to the other element. Therefore, the term "above" includes both "above" and "below" orientations depending on the spatial orientation of the device. The device may also be positioned in other ways (e.g., rotated 90 degrees or in other orientations), and the spatial relational terms used herein will be interpreted accordingly.

[0077] The terms used herein are only used to describe various examples and are not used to limit the present disclosure. Unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. The terms "include", "comprise" and "have" list the stated features, quantities, operations, components, elements and / or their combinations that exist, but do not exclude the existence or addition of one or more other features, quantities, operations, components, elements and / or their combinations.

[0078] Variations in the shapes shown in the drawings may occur due to manufacturing techniques and / or tolerances. Therefore, the examples described herein are not limited to the specific shapes shown in the drawings but include variations in shapes that occur during manufacturing.

[0079] The features of the examples described herein may be combined in various ways that will be apparent after understanding the disclosure of the present application. In addition, although the examples described herein have various configurations, other configurations are possible as will be apparent after understanding the disclosure of the present application.

[0080] See also Figures 1 to 14As shown, the following describes each component in detail from the perspective of the whole machine. The molecular detection device according to the embodiment of the present application includes a sample processing mechanism 1, a sample transfer mechanism 2 and a sample detection mechanism 3; wherein the sample processing mechanism 1 pre-processes the initial sample, that is, injects the detection reagent into the initial sample to purify the nucleic acid. In the following, the structure of the sample processing mechanism 1 will be described in detail, specifically.

[0081] The sample processing mechanism 1 includes a carrier box 11, and the carrier box 11 forms a plurality of reagent chambers, one of which can carry an initial sample as a sample processing chamber, one of which can be used as a waste liquid chamber, and the remaining reagent chambers can be used as processing liquid chambers. Among them, the sample processing liquid chamber can be used to store various reagents used for sample processing, such as lysate, cleaning solution and eluent, etc.; the sample is placed in the sample processing chamber, and the various reagents in the processing chamber are transferred to the sample processing chamber in sequence according to the prescribed steps to react with the sample, and after each step of the reaction is completed, the waste liquid generated is transferred to the waste liquid chamber to finally obtain a nucleic acid solution. In the following, reference will be made to Figures 3 to 6 A first embodiment of a carrier box is described.

[0082] like Figures 2 to 6 As shown, the sample processing mechanism 1 includes the above-mentioned carrier box 11, a pipette valve movably connected to the carrier box 11, and a pipette drive mechanism 13, wherein the pipette drive mechanism 13 can be connected to the pipette valve to drive the pipette valve to move (in the embodiment shown in the figure, for example, driving the first pipette valve 121 and the second pipette valve 122 to rotate), so that the pipette valve (including the first pipette valve 121 and the second pipette valve 122) is respectively connected to a plurality of reagent chambers, and enables reagent transfer between the pipette valve and the connected reagent chambers; the pipette valve, driven by the pipette drive mechanism 13, sequentially transfers the reagent in the processing liquid chamber to the sample processing chamber, and transfers the waste liquid generated in the sample processing chamber after each step of the reaction is completed to the waste liquid chamber, thereby conveniently and quickly obtaining a nucleic acid solution. Subsequently, the structure of the pipette valve will be described in detail.

[0083] exist Figure 3 In the embodiment shown in , there are two pipetting valves, namely, a first pipetting valve 121 and a second pipetting valve 122 . However, since the structures of the two pipetting valves are substantially the same, only the first pipetting valve 121 is taken as an example for description.

[0084] like Figure 3 and Figure 4As shown, the carrier box 11 may include a box body and a first transfer valve 121, the box body is formed with a first valve compartment and a first flow channel 116 to a fourth flow channel 119, wherein the first flow channel 116 to the fourth flow channel 119 are formed at the bottom of the box body. A first valve cavity and a first transfer port connected to the first valve cavity are formed in the first transfer valve 121. In an embodiment, the first transfer port may be formed at the bottom of the first transfer valve 121. The first transfer valve 121 may be movably connected to the first valve compartment, for example, the first transfer valve 121 may be rotated, so that the first transfer port of the first transfer valve 121 is connected to the corresponding reagent chamber and the second transfer valve 122 via the corresponding flow channel.

[0085] Preferably, in the embodiment, specifically, the first pipetting valve 121 includes a valve body and a valve stem, the valve stem is located in the valve body, and the valve stem can perform piston motion in the valve body, and the outer wall of the end of the valve stem can abut against the inner wall of the valve body to form a sealed contact. With the action of the pipetting drive mechanism 13 described below, the valve stem reciprocates in the valve body, for example, the valve stem moves upward in the valve body, and the reagent in the reagent chamber connected to the pipetting valve can be extracted into the pipetting valve, and then the valve stem moves downward in the valve body, which pushes the reagent in the pipetting valve into the reagent chamber connected thereto; the valve body is rotatably connected to the carrier box 11, and when the valve body rotates to different angles, it can be connected to multiple reagent chambers respectively. That is, during the actual operation, the first pipette valve 121 (or the second pipette valve 122) is rotated to a predetermined position through the action of the pipette drive mechanism 13, so that its pipette port is connected to the corresponding reagent chamber or the corresponding pipette valve via the corresponding flow channel. At this time, the action of the pipette drive mechanism 13 causes the valve stem to reciprocate in the valve body to complete the transfer of the reagent (i.e., extraction and discharge).

[0086] As described above, the first flow channel 116 to the fourth flow channel 119 are formed at the bottom of the box body, wherein the corresponding flow channel can connect the first transfer valve 121 with the second transfer valve 122, connect the first transfer valve 121 with the corresponding reagent chamber, or connect the second transfer valve 122 with the corresponding reagent chamber. Figure 4 Describe the structure of the flow channel in detail.

[0087] like Figure 3 and Figure 4As shown, the first valve compartment is connected with the first reagent chamber via the first flow channel 116, thereby enabling the transfer of reagent between the first pipetting valve 121 and the first reagent chamber; the second valve compartment for installing the second pipetting valve 122 and opened in the box body is connected with the first valve chamber via the second flow channel 117, thereby enabling the transfer of reagent between the first pipetting valve 121 and the second pipetting valve 122; the second valve compartment is connected with the second reagent chamber via the third flow channel 118, thereby enabling the transfer of reagent between the second valve compartment and the second reagent chamber; the second valve compartment is also connected with the liquid inlet described below via the fourth flow channel 119, thereby enabling the transfer of reagent between the second valve compartment and the test tube.

[0088] In addition, in an embodiment, one of the multiple first reagent chambers can be used as a sample processing chamber for accommodating samples to be processed; one of the multiple first reagent chambers can be used as a waste liquid chamber for accommodating waste liquid generated in each step of the reaction; the remaining first reagent chambers can be used as reagent chambers for storing various reagents for sample processing (such as lysate, cleaning solution and eluent, etc.); the second reagent chamber, for example, stores a PCR reaction system stored in the form of freeze-dried beads, including buffer, polymerase or reverse transcriptase, etc.

[0089] Here, the transfer of reagents such as lysate by using the first pipetting valve 121 will be described in detail with the help of examples. Specifically, the sample to be processed is first placed in the sample processing chamber, and then the sample processing chamber will not be opened again during the subsequent sample processing process; then, the reagents in each reagent chamber are transferred to the sample processing chamber in sequence through the first pipetting valve 121 for reaction, and the waste liquid generated by each step of the reaction is transferred to the waste liquid chamber through the first pipetting valve 121. For example, when the lysate is added to the sample processing chamber, the first pipetting valve 121 is first rotated so that the first valve chamber of the first pipetting valve 121 is connected to the reagent chamber storing the lysate, and a predetermined amount of the lysate is extracted into the first valve chamber by the action of the valve stem as described below; then the first pipetting valve 121 is continued to rotate so that the first valve chamber of the first pipetting valve 121 is connected to the sample processing chamber, and the lysate in the first valve chamber is transported to the sample processing chamber by the action of the valve stem as described below.

[0090] Here, the transfer of reagents by using the first pipette valve 121 and the second pipette valve 122 will be described in detail with the help of examples. Specifically, the second reagent chamber (i.e., the reconstitution chamber 115) stores, for example, a PCR reaction system stored in the form of freeze-dried beads, wherein the PCR reaction system includes buffer, polymerase or reverse transcriptase, etc.; when the sample processing chamber completes the nucleic acid extraction of the sample, the first pipette valve 121 is first rotated, specifically, the first valve chamber of the first pipette valve 121 is connected to the sample processing chamber, and the action of the valve stem is used to extract the nucleic acid solution after the nucleic acid extraction is completed into the first valve chamber of the first pipette valve 121; then the first pipette valve 121 and The second pipette valve 122 rotates so that the first pipette valve 121 and the second pipette valve 122 are connected through the second flow channel 117, and then the nucleic acid solution in the first valve chamber is transferred to the second valve chamber by operating the corresponding valve stem; then the second pipette valve 122 continues to rotate so that the second valve chamber of the second pipette valve 122 is connected to the second reagent chamber, and the nucleic acid solution in the second valve chamber is transferred to the second reagent chamber, so that the freeze-dried beads (such as buffer, polymerase or reverse transcriptase) in the second reagent chamber can be re-dissolved with the nucleic acid solution.

[0091] In addition, in an embodiment, the carrier box 11 may also be provided with a mounting frame 112, which is used to mount the container 111 to be detected. Specifically, the mounting frame 112 may be, for example, a test tube rack (mounting frame 112), and the container 111 to be detected is a test tube (e.g., a capillary), and a plurality of test tubes are mounted on the test tube rack (mounting frame 112). The processed sample to be detected can be injected into the test tube. That is, as described above, the transfer of reagents between the second valve compartment of the carrier box 11 and, for example, a test tube is also achieved via the fourth flow channel 119. In addition, the box body is provided with a plurality of liquid inlets as described above, and the plurality of liquid inlets correspond one-to-one to the plurality of test tubes, and the fourth flow channel 119 corresponds one-to-one to the liquid inlet.

[0092] In an embodiment, the box body is further provided with a drainage needle extending in a vertical direction, a drainage channel and an upper port and a lower port connected to the drainage channel are formed inside the drainage needle, and the lower port of the drainage needle is connected to the liquid inlet; when the mounting bracket 112 is installed on the box body, the drainage needle can be inserted into the corresponding test tube, and the upper port of the drainage needle extends to the upper end of the interior of the test tube, so that the detection solution gradually fills the test tube from the top of the test tube, such as a capillary tube, so that the gas in the test tube can be discharged during the process of gradually filling the test tube, thereby preventing the presence of bubbles in the solution and affecting the accuracy of the test result.

[0093] In addition, in the embodiment, in a specific application, the amount of template and PCR reaction system required is small. In order to transfer the template and PCR reaction system more accurately, in this embodiment, two pipette valves with different capacities can be set. The capacities of the first pipette valve 121 and the second pipette valve 122 are different, so as to transfer the reagents more accurately.

[0094] In the first embodiment of the carrier box, the number of pipetting valves is two, namely, a first pipetting valve 121 and a second pipetting valve 122. However, the number of pipetting valves is not limited thereto, namely, in the second embodiment of the sample processing device, the sample processing device may include only one pipetting valve.

[0095] In the second embodiment of the carrier cartridge, when the sample processing mechanism includes only one pipetting valve (ie, the third pipetting valve 123), for example, referring to Figure 7 , at this time, the chip board 114 can be set to be detachably connected to the carrier box 11. The chip board 114 is formed with a main channel, a branch channel and a plurality of reagent chambers, the third pipetting valve 123 is connected to one end of the main channel, the other end of the main channel is connected to each branch channel, and each branch channel is also connected to each reagent chamber respectively. After the initial sample completes the nucleic acid extraction, the nucleic acid extract can be distributed into each reagent chamber on the chip board 114 through the third pipetting valve 123, that is, the chip board 114 achieves the function of guiding the nucleic acid extract to each reagent chamber. The board surface of the chip board 114 is covered with a sealing film, which can seal and store the nucleic acid extract. At this time, the chip board 114 can be used as a container 111 to be detected. The chip board 114 can be directly removed from the carrier box 11 later for the next step of detection.

[0096] Hereinafter, the specific structure of the liquid transfer driving mechanism 13 of the sample processing mechanism 1 will be described in conjunction with the accompanying drawings.

[0097] See also Figure 2 , Figure 5 and Figure 6 As shown, preferably, the liquid transfer drive mechanism 13 includes a rotating mechanism 131 and a push-pull mechanism 132, and the rotating mechanism 131 and the push-pull mechanism 132 are arranged corresponding to the liquid transfer valves. The two rotating mechanisms 131 are respectively a first rotating mechanism connected to the first liquid transfer valve 121 and a second rotating mechanism connected to the second liquid transfer valve 122. The two push-pull mechanisms 132 are respectively a first push-pull mechanism connected to the first liquid transfer valve 121 and a second push-pull mechanism connected to the second liquid transfer valve 122.

[0098] The first rotating mechanism can be connected to the first valve body to drive the first valve body to rotate, so that the first transfer valve is respectively connected to multiple reagent chambers or corresponding second transfer valves. The first push-pull mechanism can be connected to the first valve stem to drive the first valve stem to perform piston motion (i.e., reciprocating motion) in the first valve body, thereby completing the reagent transfer between the first transfer valve and the connected reagent chambers.

[0099] The second rotating mechanism can be connected to the second valve body to drive the second transfer valve to rotate, and the second push-pull mechanism can be connected to the second valve stem to drive the second valve stem to perform piston movement in the second valve body to complete the reagent transfer between the second transfer valve and the connected chamber. The action of the second transfer valve is similar to that of the first transfer valve.

[0100] See also Figure 2 and Figure 5 As shown, in an optional solution of this embodiment, the sample processing mechanism 1 further includes a frame 14 for supporting the sample processing mechanism 1, and the frame 14 includes a first mounting plate 141, a second mounting plate 142 and a mounting platform 143 that are spaced apart, and the first mounting plate 141 is located between the second mounting plate 142 and the mounting platform 143. Subsequently, the detailed structures of the first rotating mechanism and the second rotating mechanism will be described.

[0101] like Figure 6 As shown, the first rotating mechanism and the second rotating mechanism both include a connecting shaft 1314, a rotating driving device 1311 (in the embodiment, the rotating driving device 1311 can be, for example, a motor), a main gear 1312 and a sub-gear 1313; the fixed ends of the rotating driving device 1311 are both mounted on the first mounting plate 141, and the movable end of the rotating driving device 1311 is connected to the main gear 1312 to drive the main gear 1312 to rotate. The connecting shaft 1314 passes through the first mounting plate 141 in the vertical direction and is disposed on the first mounting plate 141, and the connecting shaft 1314 is rotatably connected to the first mounting plate 141; the main gear 1312 and the sub-gear 1313 are meshed with each other, and the sub-gear 1313 is sleeved on the connecting shaft 1314. When the rotating driving device 1311 drives the main gear 1312 to rotate, the sub-gear 1313 rotates synchronously, and at the same time drives the connecting shaft 1314 to rotate synchronously. Since the connecting shaft 1314 is detachably connected to the corresponding valve bodies of the first transfer valve 121 and the second transfer valve 122, the rotation of the connecting shaft 1314 can drive the corresponding valve bodies to rotate accordingly. Specifically, a connecting portion is formed on the outer wall of the valve body, and the connecting portion is hexagonal; a connecting hole is opened at one end of the connecting shaft facing the valve body, and the connecting hole is also hexagonal and matches the connecting portion on the valve body, so that the connecting hole can be sleeved on the connecting portion; so that when the connecting shaft rotates, the valve body can rotate accordingly.

[0102] That is, the driving force is transmitted through the meshing main gear 1312 and the sub-gear 1313 and the connecting shaft, thereby driving the corresponding valve bodies of the first transfer valve 121 and the second transfer valve 122 to rotate. Generally speaking, the rotation direction is located in the horizontal plane.

[0103] Subsequently, the structures and actions of the first push-pull mechanism and the second push-pull mechanism will be described.

[0104] In the embodiment, both the first push-pull mechanism and the second push-pull mechanism are mounted on the second mounting plate 142. Specifically, the first push-pull mechanism and the second push-pull mechanism have the same structure, both including a push-pull drive device 1321 and a first electric claw 1322, and the first electric claw 1322 can be specifically an electric finger. The fixed end of the push-pull drive device 1321 is mounted on the second mounting plate 142, and the driving end of the push-pull drive device 1321 is connected to the electric finger to drive the electric finger to reciprocate in the vertical direction (i.e., the first direction, direction a in the figure). The connecting shaft 1314 passes through the first mounting plate 141, and the connecting shaft 1314 is a hollow structure so that the valve stem can extend from the connecting shaft 1314. The push-pull drive device 1321 drives the electric finger to descend to the valve stem, and the electric finger can clamp the valve stem. Then the push-pull drive device 1321 drives the electric finger to reciprocate in the first direction, driving the first valve stem to do piston movement in the first valve body, thereby completing the extraction and discharge of the reagent.

[0105] In the following, the operation process of the sample pretreatment stage is described in detail by taking the case of two pipetting valves as an example.

[0106] During the specific operation, after the nucleic acid extraction of the sample is completed in the sample processing chamber, the first rotating mechanism is first used to drive the first pipette valve 121 to rotate so that it is connected to the sample processing chamber, and the nucleic acid solution is extracted into the first pipette valve (specifically, the first push-pull mechanism as above is used to drive the first valve stem to move upward in the first valve body to extract the nucleic acid solution into the first pipette valve); then the first rotating mechanism and the second rotating mechanism are used to respectively drive the first pipette valve 121 and the second pipette valve 122 to rotate to a position where the two are connected, and the nucleic acid solution in the first pipette valve 121 is transferred to the second pipette valve 122 (the first push-pull mechanism as above is used to drive the first valve stem to move downward in the first valve body to transfer the nucleic acid solution in the first pipette valve 121 to the second pipette valve 122); then the second rotating mechanism is used to drive the second pipette valve 122 to rotate to the position where the two are connected. At the position where the chamber 115 is connected, the nucleic acid solution is pushed into the re-dissolution chamber 115 (using the second push-pull mechanism as above to drive the second valve stem to move downward in the second valve body to push the nucleic acid solution into the re-dissolution chamber 115); after the re-dissolution reaction is completed, the second pipette valve is driven to extract the reagent in the re-dissolution chamber 115 into the second pipette valve (using the second push-pull mechanism as above to drive the second valve stem to move upward in the second valve body to extract the reagent in the re-dissolution chamber 115 into the second pipette valve), and then the second rotating mechanism is used to connect the second pipette valve 122 with multiple test tubes in turn, and the reagent in the second pipette valve 122 is respectively transported to multiple test tubes (using the second push-pull mechanism as above to drive the second valve stem to move downward in the second valve body to transport the reagent in the second pipette valve to multiple test tubes), thereby completing the filling of the test tubes for subsequent testing.

[0107] In addition, in the examples, see Figure 3 As shown, in an optional solution of this embodiment, the frame 14 is further provided with a guide rail and a carrier box driving device, and the carrier box driving device can drive the carrier box 11 to move along the guide rail. In the embodiment, the carrier box driving device can be, for example, a motor. In the initial test state, the carrier box 11 is at one end of the guide rail close to the loading port to facilitate the initial sample to be placed in the carrier box 11. After the initial sample is placed, the carrier box 11 moves to the other end of the guide rail under the drive of the carrier box driving device. The movement direction of the carrier box 11 is the second direction (direction b in the figure). The carrier box 11 moves to the subsequent mechanism to achieve pretreatment of the initial sample.

[0108] See also Figure 5As shown, a spacing adjustment mechanism 144 (for example, a motor) is further provided between the first mounting plate 141 and the mounting platform 143 of the frame 14, and the spacing adjustment mechanism 144 is used to lower or raise the first mounting plate 141. Specifically, the spacing adjustment mechanism 144 can first drive the first mounting plate 141 to move up to the standby position, and place the carrier box 11 at the sample processing position below the first mounting plate 141. The spacing adjustment mechanism 144 drives the first mounting plate 141 to move down to the operating position, so that the lower end of the corresponding connecting shaft 1314 of the rotating mechanism is connected to the corresponding valve body. After the reagent transfer is completed, the rotating mechanism is lifted, so that the lower end of the corresponding connecting shaft 1314 is disconnected from the corresponding valve body.

[0109] See also Figure 8 As shown, preferably, the sample processing mechanism 1 also includes an auxiliary mechanism 15, which includes an ultrasonic generator 151, a magnetic device 152 and a turntable member 154 connected to the frame 14. The ultrasonic generator 151 and the magnetic device 152 are used to further purify the nucleic acid of the sample. Among them, the ultrasonic treatment of a specific frequency and power can effectively help the cell breakage of pathogens and the mixing of various reaction solutions; in the process of nucleic acid extraction, the magnetic device 152 is used to absorb magnetic beads to prevent the magnetic beads from flowing into the processed sample and affecting the detection effect.

[0110] Specifically, the ultrasonic generator 151 and the magnetic device 152 are installed on a horizontally rotating turntable member 154. The turntable member 154 rotates so that the ultrasonic generator 151 and the magnetic device 152 are respectively close to the bottom of the carrier box 11 to process the sample. A cam 155 and a cam driving device 156 (for example, a motor) are provided at the bottom of the turntable member 154. The cam 155 rotates under the drive of the cam driving device 156, thereby pushing the turntable member 154 to move in the vertical direction, so that the transducer of the ultrasonic generator 151 can abut against the bottom wall of the carrier box 11, and the magnetic device 152 abuts against the side wall of the carrier box 11, so as to achieve a better processing effect. In some cases, the sample processing process requires a certain temperature, and the heating element 153 provided at the top of the ultrasonic generator 151 can provide heat for the reaction in the sample processing chamber.

[0111] In addition, in the following, we will combine Figure 3 and Fig. 9 The structure and operation of the sample transfer mechanism 2 of the molecular detection device are described.

[0112] Specifically, in the embodiments, see Figure 3 and Fig. 9As shown, the sample transfer mechanism 2 includes a second electric gripper 21, a flipping mechanism 22, a translation mechanism 23, a lifting mechanism 24 and a support frame 25 for supporting. The second electric gripper 21 can also be, for example, an electric finger. In the pretreatment stage, the test tube is mounted on the test tube rack (mounting frame 112), the test tube mouth is facing downward and connected to the test tube rack (mounting frame 112) provided on the carrier box 11. In the detection stage, the test tube containing the sample needs to be placed in the heating tank 311 and the optical tank 321 described below, so as to detect the sample. The second electric gripper 21 is used to clamp the test tube rack. The flipping mechanism 22 is connected to the second electric gripper 21 to drive the second electric gripper 21 to rotate, so that the test tube rack drives the test tube to flip, so that the test tube mouth faces upward, so that the test tube can be placed in the heating tank 311 and the optical tank 321. The flipping mechanism 22 rotates the second electric gripper 21, and also moves the test tube to the sample detection mechanism 3.

[0113] After the sample is injected into the test tube, the test tube opening needs to be sealed, that is, the test tube opening needs to be capped. In this embodiment, the carrier box 11 is provided with a sealing cover plate 113, and the sealing cover plate 113 is detachably connected to the carrier box 11; a sealing plug is provided on the sealing cover plate 113, and the sealing plug can seal the test tube opening. Both the translation mechanism 23 and the lifting mechanism 24 are fixed to the housing. Fig. 9 In order to clearly show the internal structure of the sample transfer mechanism 2, the illustration of the housing is omitted. After the second electric claw 21 clamps the test tube rack (mounting frame 112), the driving end of the lifting mechanism 24 drives the support frame 25 to rise through the first connecting rod, thereby driving the test tube rack (mounting frame 112) to rise, the purpose of which is to remove the test tube rack (mounting frame 112) from the carrier box 11; then the driving end of the translation mechanism 23 translates the support frame 25 along the guide rod 26 through the second connecting rod, so that the test tube rack (mounting frame 112) is translated to the sealing cover plate 113, and then the lifting mechanism 24 drops the test tube rack (mounting frame 112) so that the test tube rack (mounting frame 112) fits with the sealing cover plate 113, and the test tube mouth is sealed with a sealing plug. After the test tube mouth is sealed, the lifting mechanism 24 and the translation mechanism 23 move the test tube rack so that the test tube rack is away from the carrier box 11, thereby leaving space for the flipping mechanism 22 to flip the test tube rack. The flipping mechanism 22 drives the second electric claw 21 to rotate, thereby flipping the test tube rack so that the mouth of the test tube faces upward, and then the test tube rack is moved to the sample detection mechanism through the lifting mechanism 24 and the translation mechanism 23, so that the test tube rack is connected to the sample detection mechanism.

[0114] In addition, in the following, we will combine Figures 10 to 14 Describe the structure and operation of the sample detection mechanism 3 of the molecular detection device. Fig.10As shown, in an optional solution of this embodiment, the sample detection mechanism 3 includes a heating device 31, an optical detection device 32 and a transfer member 33; the sample transfer mechanism 2 can move the sample to the transfer member 33 so that the test tube rack (mounting frame 112) is connected to the transfer member 33. Fig.13 As shown, the transfer member 33 includes a motion device, a mechanical arm and a shock absorbing member; the motion device includes a first guide rail 331 and a second guide rail 332 which are perpendicular to each other and two moving drive devices 333 corresponding to the first guide rail 331 and the second guide rail 332, so as to drive the mechanical arm to move in the horizontal direction and the vertical direction. The mechanical arm 334 is provided with a receiving platform, on which the test tube rack can be overlapped, and the test tube rack and the receiving platform are connected by pins.

[0115] The heating device 31 includes a plurality of heating tanks 311 (corresponding to the heating part), and the transfer member 33 transports the test tube into the heating tanks 311, so that the plurality of heating tanks 311 heat the sample to be detected to different temperatures, thereby achieving reverse transcription (55 degrees Celsius), DNA degradation (105 degrees Celsius) and cooling (15 degrees Celsius); when the sample to be detected is heated to the DNA extension temperature (60 degrees Celsius), the transfer member 33 transports the test tube to the optical detection device 32. Generally speaking, the sample needs to cycle through the reverse transcription, DNA degradation and cooling processes for multiple times.

[0116] In an optional solution of this embodiment, a shock absorbing member is provided on the transfer member 33; when the transfer member 33 transports the test tube, the shock absorbing member is used to dampen the movement of the test tube to avoid vibration caused by rapid movement of the sample to be tested, thereby improving the detection efficiency of the device. Specifically, a double spring shock absorber can be provided on the transfer member 33 so that the vibration energy is quickly absorbed by the spring.

[0117] See also Fig.11 and Fig.12 As shown, the optical detection device 32 includes an optical slot 321, a light source assembly 322 and an imaging assembly 323. The optical slot 321 is provided with an insertion port, and the container 111 to be detected (for example, in this embodiment, the container 111 to be detected can be a test tube for containing nucleic acid) can enter the optical slot 321 from the insertion port (for example, via a clamp connected to a robotic arm, such as the robotic arm described above) for subsequent nucleic acid detection operations.

[0118] In this embodiment, the optical slot 321 may be provided with a first through hole (corresponding to the first light-transmitting portion) and a second through hole (corresponding to the second light-transmitting portion), and the light emitted by the light source assembly 322 can be incident from the first through hole into the optical slot 321, so that the required light is irradiated on the test tube; in addition, the light in the optical slot 321 can pass through the second through hole and be incident into the imaging assembly 323 for subsequent imaging operations. A reflector is provided in the optical slot to change the route of the light so that every part of the sample to be detected is irradiated to improve the detection effect.

[0119] In an embodiment, the light source assembly 322 can move relative to the optical slot 321, and the light source assembly 322 can include multiple illumination light sources for emitting light of different wavelengths. The light source assembly 322 can switch each illumination light source so that any illumination light source can move to the first through hole; the imaging assembly 323 can capture imaging images of the test tube under light of different wavelengths to perform multiple fluorescence detection. In an embodiment, the multiple illumination light sources are arranged in a row.

[0120] See also Fig.14 As shown, or a light source assembly 322 having multiple irradiation light sources is installed on the second side wall of the optical groove 321 to emit light of different wavelengths. Preferably, the number of second side walls is two, and the two second side walls are arranged opposite to each other. Light source assemblies 322 are respectively installed on the opposite second side walls to emit light of different wavelengths (only one second side wall is shown in the figure, and the second side wall on the opposite side is not shown). When one of the light source assemblies 322 fails, the other light source assembly 322 can still work so that the instrument does not need to be shut down; at least a reflector is arranged on the side wall of the optical groove 321 opposite to the light source assembly 322, so that the side of the sample to be detected away from the light source can also have sufficient light. When the two second side walls arranged opposite to each other are both provided with light source assemblies 322, reflectors are arranged on the two second side walls so that the light emitted by the light source assembly 322 can be reflected by the reflector in the optical groove 321 to different positions of the sample to be detected, thereby improving the detection effect.

[0121] In addition, in an embodiment, the light source assembly 322 can provide light of different wavelengths. Specifically, the light source assembly 322 is provided with illumination light sources of different colors, such as green, yellow, amber, blue, etc. A plurality of illumination light sources are arranged in a row or array. Fig.11 and Fig.12 In the embodiment of the invention, the multiple illumination light sources of the light source assembly 322 can be switched to the corresponding illumination light sources for irradiating the container to be inspected by driving the slider along the track by a motor, and the specific structure of switching the multiple illumination light sources is not described in detail here. Fig.14 In the embodiment, the plurality of illumination light sources may be controlled by a controller to light up separately.

[0122] like Fig.12 As shown, the imaging component 323 includes a filter 3231 and a shooting component 3232 (such as a CMOS camera); the shooting component 3232 is arranged opposite to the second through hole; the filter 3231 is arranged between the shooting component 3232 and the optical slot 321, and the filter 3231 can move relative to the optical slot 321.

[0123] In this embodiment, since the light source assembly 322 provides light of different wavelengths, the imaging assembly 323 is correspondingly provided with an optical filter of the same color as the color of the irradiation light source, which is used to filter out the noise, that is, the filter 3231 is composed of a plurality of optical filters. When the light source assembly 322 moves to switch the irradiation light source, the imaging assembly 323 switches the optical filter accordingly. The imaging assembly 323 captures the imaging images under different wavelengths of light for rapid acquisition, thereby completing multiple fluorescence detection.

[0124] As described above, the optical filter 3231 includes a plurality of optical filters, any of which can be moved to the second through hole; the optical filters correspond to the illumination light sources one by one, and the light of different wavelengths emitted by the illumination light sources can pass through the corresponding optical filters and enter the shooting component 3232. Similar to the plurality of illumination light sources, the plurality of optical filters are arranged in a row. The switching method of the optical filters is similar to Fig.11 and Fig.12 The switching method of the middle illumination light source is similar and will not be described in detail here.

[0125] In addition, a condenser 3211 is provided in the optical groove 321, and the condenser 3211 is installed at the first through hole, and is used to converge the light emitted by the light source assembly 322 to the test tube. In this embodiment, the condenser 3211 is preferably a convex lens, and more preferably a convex lens covered at the first through hole, and a convex lens is provided at the first through hole to refract the light. For the divergent light, it can be achieved that the light can be converged to the test tube as much as possible while the light passes through.

[0126] In addition, in the embodiment, the convex lens can be, for example, a biconvex lens, a crescent lens, or a planoconvex lens. However, the condenser 3211 is not limited thereto, and the condenser 3211 can also adopt other shapes or structures as long as it can realize converging the light emitted by the light source assembly 322 to the test tube.

[0127] Hereinafter, the operation of the optical detection device 32 according to the embodiment of the present application will be described.

[0128] Specifically, in the molecular diagnosis process, at each cycle of DNA extension temperature, the test tube will move into the optical slot 321 to collect fluorescence signals. The light source assembly 322 can emit light, which enters the optical slot 321 from the first through hole and irradiates the test tube, excites the substances in the test tube (such as dyes and probes), and realizes fluorescence imaging through the specific imaging assembly 323 arranged at the second through hole, completing rapid collection. In order to allow the light emitted by the light source assembly 322 to irradiate more of the test tube, the focusing member 3211 arranged at the first through hole can effectively change the path of the light emitted by the light source assembly 322, thereby changing the divergent state of the light, so that the light converges to the test tube, thereby effectively improving the detection efficiency.

[0129] According to the molecular detection equipment provided in the present application, the sample is pre-processed, transferred and fluorescently detected in sequence through the sample processing mechanism 1, the sample transfer mechanism 2 and the sample detection mechanism 3, thereby realizing automation and integration of the detection process, improving detection efficiency and saving labor costs.

[0130] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them; although the present application is described in detail with reference to the above embodiments, a person skilled in the art should understand that the technical solutions described in the above embodiments can still be modified, or some or all of the technical features can be replaced by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application. In addition, a person skilled in the art can understand that although some embodiments herein include certain features included in other embodiments but not other features, the combination of features of different embodiments means being within the scope of the present application and forming different embodiments.

Claims

1. A sample detection mechanism, characterized in that: It includes a heating device, an optical detection device and a transfer member; The heating device is formed with a heating part, and the heating part is used for cyclically heating the sample to be detected; The transfer member is used to transfer the sample to be detected between the heating part and the optical detection device; The optical detection device is used to perform optical detection on the sample to be detected that has completed the thermal cycle; The optical detection device comprises an optical slot, a light source assembly and an imaging assembly; the optical slot is provided with an inlet, and the sample to be detected can enter the optical slot from the inlet; one or more reflectors are arranged inside the optical slot, and the reflectors are used to reflect the light in the optical slot; the light emitted by the light source assembly can be reflected by the reflectors in the optical slot to different positions of the sample to be detected; The optical groove has a first side wall, a first light-transmitting portion is formed on the first side wall, and light in the optical groove can pass through the first light-transmitting portion and enter the imaging component; A light concentrator is disposed in the optical groove and is installed at the first light-transmitting portion to converge the light emitted by the light source assembly to the sample to be detected.

2. The sample detection mechanism according to claim 1, characterized in that: The optical slot has a second side wall, a second light-transmitting portion is formed on the second side wall, the light source assembly can move relative to the optical slot, the light source assembly includes a plurality of illumination light sources arranged in a row, any of the illumination light sources can move to the second light-transmitting portion, and the plurality of illumination light sources are used to emit light of different wavelengths; The imaging component can capture imaging images of the sample to be detected under light of different wavelengths.

3. The sample detection mechanism according to claim 1, characterized in that: The optical groove has a second side wall, and the light source assembly is installed on the second side wall; The light source assembly includes a plurality of illumination light sources, and the plurality of illumination light sources are used to emit light of different wavelengths; the imaging assembly can capture imaging images of the sample to be detected under light of different wavelengths.

4. The sample detection mechanism according to claim 1, characterized in that: The optical groove has a second side wall, the number of the second side walls is two, the two second side walls are arranged opposite to each other in the optical groove, and the light source assembly is installed on both the two second side walls; The light source assembly includes a plurality of illumination light sources, and the plurality of illumination light sources are used to emit light of different wavelengths; the imaging assembly can capture imaging images of the sample to be detected under light of different wavelengths.

5. The sample detection mechanism according to any one of claims 2 to 4, characterized in that: When there are multiple reflectors in the optical groove, at least one reflector is disposed on the side wall of the optical groove opposite to the light source assembly.

6. The sample detection mechanism according to any one of claims 2 to 4, characterized in that: The imaging assembly includes a filter and a shooting device; the shooting device is arranged opposite to the first light-transmitting portion; The optical filter is arranged between the shooting device and the optical slot, and the optical filter can move relative to the optical slot; the optical filter includes a plurality of optical filters arranged in a row, and any of the optical filters can move to the first light-transmitting portion; The optical filters correspond to the illumination light sources one by one, and the light rays of different wavelengths emitted by the illumination light sources can respectively pass through the corresponding optical filters and enter the shooting component.

7. The sample detection mechanism according to claim 1, characterized in that: The transfer member arrangement includes a motion device, a mechanical arm and a shock absorbing member; The motion device is connected to the robotic arm to drive the robotic arm to move; the robotic arm is used to carry the sample to be detected; the robotic arm is provided with the shock absorbing component, and when the transfer component transports the sample to be detected, the shock absorbing component is used to absorb the movement of the sample to be detected.

8. A molecular detection device, characterized in that: It comprises a sample processing mechanism, a sample transfer mechanism and a sample detection mechanism as described in any one of claims 1 to 7; The sample processing mechanism is capable of carrying samples to be tested; The sample transfer mechanism is used to transport the sample to be tested to the sample testing mechanism; The sample detection mechanism is used to perform nucleic acid detection on the sample to be detected.

9. The molecular detection device according to claim 8, characterized in that The sample processing mechanism includes a carrying box and a pipetting valve assembly; The carrying box is provided with a plurality of reagent chambers, the pipetting valve assembly is movably connected to the carrying box, and the pipetting valve assembly can move so as to communicate with the corresponding reagent chambers.

10. The molecular detection device according to claim 9, characterized in that The pipetting valve assembly includes at least one pipetting valve; The pipetting valve comprises a valve body and a valve stem connected to each other; the valve body is rotatably connected to the carrier box, and the valve body can be respectively connected to the plurality of reagent chambers; the valve stem can reciprocate relative to the valve body along a first direction to change the pressure in the valve body, so that the valve body absorbs or discharges the detection reagent and the sample to be detected; When there are multiple pipetting valves, the multiple pipetting valves can be connected to each other, so that the detection reagent and the sample to be detected can be transferred between the multiple pipetting valves.

11. The molecular detection device according to claim 10, characterized in that: The sample processing mechanism also includes a pipetting drive mechanism, which includes a rotating mechanism and a push-pull mechanism arranged corresponding to the pipetting valve; the rotating mechanism is used to drive the valve body of the pipetting valve to rotate, and the push-pull mechanism is used to push and pull the valve stem reciprocatingly along a first direction.

12. The molecular detection device according to claim 11, characterized in that The rotating mechanism comprises a rotating driving device, a connecting shaft, and a main gear and a sub-gear in meshing connection; the output end of the rotating driving device is connected to the main gear, one end of the connecting shaft is connected to the sub-gear, and the other end of the connecting shaft is detachably connected to the valve body to drive the valve body to rotate; The push-pull mechanism includes a push-pull driving device and a first electric claw for clamping the valve stem. The output end of the push-pull driving device is connected to the first electric claw to drive the first electric claw to approach or move away from the valve stem.

13. The molecular detection device according to claim 11 or 12, characterized in that: The sample processing mechanism further comprises a frame, and the carrying box and the liquid transfer driving mechanism are both mounted on the frame; The frame is also provided with a guide rail and a carrier box driving device; the carrier box is slidably connected to the guide rail via a slide, and the driving end of the carrier box driving device is connected to the slide, and the carrier box can reciprocate along a second direction to approach or move away from the pipetting drive mechanism; the second direction is perpendicular to the first direction.

14. The molecular detection device according to claim 11 or 12, characterized in that: The sample processing mechanism further comprises an auxiliary mechanism, and the auxiliary mechanism is located at a side of the carrying box away from the pipetting drive mechanism; The auxiliary mechanism includes an ultrasonic generator and a magnetic attraction device; the ultrasonic generator and the magnetic attraction device can move relative to the carrying box on a plane perpendicular to the height direction of the carrying box to successively approach or move away from the carrying box; The ultrasonic generator and the magnetic device can simultaneously reciprocate along the height direction of the carrying box, so that the transducer of the ultrasonic generator fits with the bottom wall of the carrying box, and the magnetic device fits with the side wall of the carrying box; The ultrasonic generator is provided with a heating element, and the heating element is used to fit with the bottom wall of the carrying box to provide heat.

15. The molecular detection device according to any one of claims 9 to 12, characterized in that The carrying box is equipped with a container to be tested, and the pipetting valve assembly can be connected to the container to be tested so as to inject the sample to be tested into the container to be tested; The sample transfer mechanism includes a second electric claw, a flip mechanism, a translation mechanism and a lifting mechanism; The second electric clamp is used to clamp the container to be tested, and the flipping mechanism is connected to the second electric clamp to rotate the second electric clamp, thereby flipping the test container and moving it to the sample testing mechanism; The carrying box is provided with a mounting frame and a sealing cover plate, the mounting frame and the sealing cover plate are both detachably connected to the carrying box, the detection container is mounted on the mounting frame, and the sealing cover plate can seal the opening of the detection container; The lifting mechanism is connected to the flipping mechanism and is used to raise or lower the detection container; the translation mechanism is connected to the lifting mechanism and can move the detection container from the mounting frame to the sealing cover plate.

16. The molecular detection device according to claim 15, characterized in that The sample transfer mechanism comprises a support frame; The flipping mechanism is installed on the supporting frame, and the driving end of the flipping mechanism is connected to the second electric claw; The lifting mechanism includes a lifting drive device and a first connecting rod, and the lifting drive device is connected to the support frame through the first connecting rod; the translation mechanism includes a translation drive device and a second connecting rod, and the translation mechanism is connected to the lifting drive device through the second connecting rod.

Images