An anti-interference measurement method and a sample analyzer

Abstract

The embodiment of the present application discloses an anti-interference measuring method and a sample analyzer, which can save sample consumption and improve the detection range of the concentration of interfering substances in the sample under the condition of meeting the detection accuracy. The method comprises the following steps: sucking the sample to be detected; adding part of the sample into a first container to prepare a first mixed solution of the sample and a detection reagent; adding another part of the sample into a second container to prepare a second mixed solution of the sample and a diluent; irradiating the first mixed solution and the second mixed solution by using a multi-wavelength light source; collecting a first optical detection signal of the first mixed solution and a second optical detection signal of the second mixed solution; performing interfering substance analysis according to the second optical detection signal to obtain an interfering substance analysis result of the sample; and performing analysis of a detection item of the sample according to the first optical detection signal and the interfering substance analysis result to obtain a detection result of the detection item of the sample.

Description

Technical Field

[0001] This invention relates to the field of sample analysis technology, and in particular to an anti-interference measurement method and a sample analyzer. Background Technology

[0002] In optical methods for detecting coagulation, the absorbance of the solution is typically obtained by irradiating the solution in the reaction vessel with collimated light and analyzing the scattered or transmitted light, thereby determining the coagulation time or the concentration of the analyte.

[0003] Normal blood plasma is typically pale yellow, transparent, and has very low absorbance. However, samples with obvious absorption peaks, such as those from jaundice, hemolysis, or chyle, can significantly affect transmittance and thus impact measurements, posing a challenge to the reliability of optical methods. Therefore, it is necessary to pre-detect the concentration of interfering substances in the sample before testing to mitigate their impact on measurements.

[0004] Since the three interfering substances (hemolysis, jaundice, and lipemia) have different absorption spectra in the visible light band, optical absorbance is usually used to quantitatively or semi-quantitatively detect the interfering substances.

[0005] Normally, a collimated beam is used to irradiate the plasma and the type and content of interfering substances are determined by analyzing the plasma absorbance. CN201110290915.7 uses a fractional cup method for detection, in which a sufficient amount of sample is drawn into a reaction cup for interfering substance detection, and then a sample is drawn from that reaction cup and added to another reaction cup for normal coagulation testing.

[0006] This method has the following drawbacks:

[0007] Reaction cup waste: Each sample test wastes a reaction cup, which increases user costs and also affects testing speed.

[0008] Plasma waste: If the plasma is only used for one or a few tests, the amount of sample in the reaction vessel is more than the amount required for the test, and more sample is needed, resulting in sample waste.

[0009] The detection range is very narrow: because the test is conducted using only the raw pulp, the concentration of interfering substances in the raw pulp cannot be detected when it exceeds the detection limit of the instrument. Summary of the Invention

[0010] This invention provides an anti-interference measurement method and a sample analyzer, which measures interfering substances in a diluted sample by acquiring optical signals. This method can save sample volume and increase the detection range of interfering substance concentration in the sample while meeting detection accuracy requirements.

[0011] The first aspect of this application provides an anti-interference measurement method, including:

[0012] Collect the sample to be tested;

[0013] A portion of the sample is added to the first container to prepare a first mixture of the sample and the detection reagent;

[0014] Another portion of the sample is added to the second container to prepare a second mixture of the sample and diluent;

[0015] The first mixture and the second mixture are irradiated using a multi-wavelength light source;

[0016] Collect the first optical detection signal of the first mixture and the second optical detection signal of the second mixture;

[0017] Interference analysis is performed based on the second optical detection signal to obtain the interference analysis results of the sample;

[0018] The sample is analyzed based on the first optical detection signal and the analysis results of the interference to obtain the detection results of the sample.

[0019] Preferably, the first container is a reaction vessel and the second container is a colorimetric cell;

[0020] The step of adding another portion of the sample to the second container to prepare a second mixture of the sample and diluent includes:

[0021] Add half of the preset total amount of diluent to the colorimetric cell;

[0022] Add another portion of the sample to the colorimetric cell;

[0023] Then add the other half of the diluent to the colorimetric cell to mix the sample and the diluent in the colorimetric cell.

[0024] Preferably, the method further includes:

[0025] After the measurement is completed, the colorimetric cell is cleaned.

[0026] Preferably, the step of analyzing the sample's detection items based on the first optical detection signal and the interference analysis results to obtain the detection results of the sample's detection items includes:

[0027] Analyze the detection information corresponding to different wavelengths based on the first optical detection signal;

[0028] Based on the analysis results of the interference, a corresponding wavelength is selected, and the detection information corresponding to the selected wavelength is analyzed to obtain the detection results of the detection items of the sample.

[0029] Preferably, the sample is plasma, and the interfering agent includes at least one of lipemia, hemolysis, and jaundice;

[0030] The step of irradiating the first mixture and the second mixture using a multi-wavelength light source includes:

[0031] The first mixture and the second mixture are irradiated with light sources of wavelengths of 405nm, 545nm, 660nm and 800nm.

[0032] Preferably, the step of performing interference analysis based on the second optical signal to obtain the interference analysis results of the sample includes:

[0033] The absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm were obtained respectively.

[0034] The lipid level in the plasma is calculated and analyzed according to the first formula, which is:

[0035]

[0036] Where ΔAbs_L represents the difference in absorbance of lipemia in the second mixture at wavelengths of 660 nm and 800 nm, C represents the lipemia grade coefficient, and L Index This indicates the level of lipid levels.

[0037] Preferably, the step of performing interference analysis based on the second optical signal to obtain the interference analysis results of the sample further includes:

[0038] Obtain the absorbance values ​​of the hemolyzed solution in the second mixture at wavelengths of 545 nm and 660 nm.

[0039] The hemolysis level in the plasma was calculated and analyzed according to the second formula, which is:

[0040]

[0041] Where ΔAbs_H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, A represents the hemolysis grade coefficient, ΔAbs_L represents the absorbance difference of lipohyperfusion in the second mixture at wavelengths of 660nm and 800nm, E represents the correction coefficient for lipohyperfusion when calculating the hemolysis grade, and H represents the hemolysis grade. Index Indicates the degree of hemolysis.

[0042] Preferably, the step of performing interference analysis based on the second optical signal to obtain the interference analysis results of the sample further includes:

[0043] Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm.

[0044] The jaundice level in the plasma was calculated and analyzed according to the third formula, which is:

[0045]

[0046] Wherein, ΔAbs_I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the hemolysis correction factor when calculating jaundice, ΔAbs_H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbs_L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the lipemia correction factor when calculating the hemolysis grade, G represents the lipemia correction factor when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the level of jaundice.

[0047] A second aspect of this application provides a sample analyzer, comprising:

[0048] The first container is used to hold the first mixture prepared from a portion of the sample and the detection reagents;

[0049] The second container is used to hold the second mixture prepared from another portion of the sample and the diluent;

[0050] An optical device is used to irradiate the first mixture and the second mixture using a multi-wavelength light source;

[0051] A signal acquisition device is used to acquire a first optical detection signal of a first mixture and a second optical detection signal of a second mixture.

[0052] A processor is configured to perform interference analysis based on a second optical signal to obtain interference analysis results for the sample;

[0053] The sample is analyzed based on the first optical detection signal and the analysis results of the interference to obtain the detection results of the sample.

[0054] Preferably, the first container is a reaction vessel and the second container is a colorimetric cell;

[0055] The colorimetric cell is specifically used for:

[0056] Add half the preset total amount of diluent;

[0057] Add another portion of the sample;

[0058] Add the other half of the diluent and mix the sample and diluent thoroughly.

[0059] Preferably, the sample analyzer further includes:

[0060] A cleaning device is used to clean the colorimetric cell after the measurement is completed.

[0061] Preferably, the processor is specifically used for:

[0062] Analyze the detection information corresponding to different wavelengths based on the first optical detection signal;

[0063] Based on the analysis results of the interference, a corresponding wavelength is selected, and the detection information corresponding to the selected wavelength is analyzed to obtain the detection results of the detection items of the sample.

[0064] Preferably, the sample is plasma, and the interfering agent includes at least one of lipemia, hemolysis, and jaundice;

[0065] The multi-wavelength light source includes light sources with wavelengths of at least 405nm, 545nm, 660nm and 800nm.

[0066] Preferably, the processor is specifically used for:

[0067] Obtain the absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm;

[0068] The lipid level in the plasma is calculated and analyzed according to the first formula, which is:

[0069]

[0070] Where ΔAbs_L represents the difference in absorbance of lipemia in the second mixture at wavelengths of 660 nm and 800 nm, C represents the lipemia grade coefficient, and L Index This indicates the level of lipid levels.

[0071] Preferably, the processor is further configured to:

[0072] Obtain the absorbance values ​​of hemolysis in the second mixture at wavelengths of 545 nm and 660 nm.

[0073] The hemolysis level in the plasma was calculated and analyzed according to the second formula, which is:

[0074]

[0075] Where ΔAbs_H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, A represents the hemolysis grade coefficient, ΔAbs_L represents the absorbance difference of lipohyperfusion in the second mixture at wavelengths of 660nm and 800nm, E represents the correction coefficient for lipohyperfusion when calculating the hemolysis grade, and H represents the hemolysis grade. Index Indicates the degree of hemolysis.

[0076] Preferably, the processor is further configured to:

[0077] Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm.

[0078] The jaundice level in the plasma was calculated and analyzed according to the third formula, which is:

[0079]

[0080] Wherein, ΔAbs_I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the hemolysis correction factor when calculating jaundice, ΔAbs_H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbs_L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the lipemia correction factor when calculating the hemolysis grade, G represents the lipemia correction factor when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the level of jaundice.

[0081] Preferably, the sample analyzer is a coagulation analyzer.

[0082] This application also provides a readable computer storage medium having a computer program stored thereon, which, when executed by a processor, is used to implement the anti-interference measurement method provided in the first aspect of the embodiments of this application.

[0083] As can be seen from the above technical solutions, the embodiments of the present invention have the following advantages:

[0084] In this embodiment, a sample to be tested is aspirated; a portion of the sample is added to a first container to prepare a first mixture of the sample and the detection reagent; another portion of the sample is added to a second container to prepare a second mixture of the sample and the diluent; the first mixture and the second mixture are irradiated using a multi-wavelength light source; a first optical detection signal of the first mixture and a second optical detection signal of the second mixture are acquired; interference analysis is performed based on the second optical signal to obtain the interference analysis result of the sample; and the detection items of the sample are analyzed based on the first optical detection signal and the interference analysis result to obtain the detection result of the sample. Because this embodiment utilizes a multi-wavelength light source to irradiate the second mixture prepared from the sample and the diluent, and acquires the second optical detection signal of the second mixture to analyze the interference in the sample, i.e., by measuring the interference in the diluted sample through optical signal acquisition, it can save sample volume and improve the detection range of interference concentration in the sample while meeting detection accuracy requirements. Attached Figure Description

[0085] Figure 1 This is a schematic diagram of the sample analyzer in one embodiment of this application;

[0086] Figure 2 This is another structural schematic diagram of the sample analyzer in the embodiments of this application;

[0087] Figure 3 This is another structural schematic diagram of the sample analyzer in the embodiments of this application;

[0088] Figure 4 This is a schematic diagram of one embodiment of the anti-interference measurement method in this application.

[0089] Figure 5 Examples of embodiments in this application Figure 4 Detailed steps of step 403 in the embodiment;

[0090] Figure 6 Examples of embodiments in this application Figure 4 Detailed steps of step 406 in the embodiment;

[0091] Figure 7 These are the absorbance spectra of three different interfering substances in the embodiments of this application;

[0092] Figure 8 Examples of embodiments in this application Figure 4 Detailed steps of step 407 in the embodiment;

[0093] Figure 9 This is a schematic diagram of one embodiment of the sample analyzer in this application. Detailed Implementation

[0094] This invention provides an anti-interference measurement method and a sample analyzer, which measures interfering substances in a diluted sample by acquiring optical signals. This method can save sample volume and increase the detection range of interfering substance concentration in the sample while meeting detection accuracy requirements.

[0095] To enable those skilled in the art to better understand the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

[0096] The terms "first," "second," "third," "fourth," etc., used in the specification, claims, and accompanying drawings of this invention are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments described herein can be implemented in orders other than those illustrated or described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover a non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0097] Before detailing the invention, the structure of the sample analyzer will be described first. Please refer to [link / reference needed]. Figure 1 In one embodiment, the sample analyzer may include a sample component 10, a reagent component 20, a measurement component 30, a memory 40, and a processor 50; in some embodiments, please refer to Figure 2 The sample analyzer may also include a display and operation unit 60; this will be described in detail below.

[0098] The sample component 10 is used to hold the sample in the test solution and, after aspirating the sample, provides it to the measurement component 30. Please refer to... Figure 3 In some embodiments, sample component 10 may include sample carrying component 11 and sample dispensing mechanism 12. Sample carrying component 11 is used to carry samples. In some examples, sample carrying component 11 may include sample delivery module (SDM) and front-end track; in other examples—for example Figure 3In such an example, the sample carrier 11 could also be a sample tray, which includes multiple sample positions for placing sample tubes. By rotating its tray structure, the sample can be moved to the appropriate position, such as the position for the sample dispensing mechanism 12 to pick up the sample. The sample dispensing mechanism 12 is used to pick up the sample and dispense it into the reaction cup to be added. For example, the sample dispensing mechanism 12 may include a sample needle, which is driven by a two-dimensional or three-dimensional mechanism to move in two-dimensional or three-dimensional space, thereby moving the sample needle to pick up the sample carried by the sample carrier 11, and to move to the reaction cup to be added, and dispense the sample into the reaction cup.

[0099] The reagent component 20 is used to carry the reagent in the test solution and, after aspirating the reagent, provides it to the measuring component 30. In some embodiments, the reagent component 20 may include a reagent carrying component 13 and a reagent dispensing mechanism 14. The reagent carrying component 13 is used to carry the reagent. In one embodiment, the reagent carrying component 13 may be a reagent tray, which is arranged in a disc-shaped structure and has multiple positions for carrying reagent containers. The reagent carrying component 13 is rotatable and drives the reagent container it carries to rotate, for rotating the reagent container to a specific position, such as the position where the reagent dispensing mechanism 14 aspirates the reagent. The number of reagent carrying components 13 may be one or more. The reagent dispensing mechanism 14 is used to aspirate the reagent and discharge it into a reaction cup to which the reagent is to be added. In one embodiment, the reagent dispensing mechanism 14 may include a reagent needle, which is driven by a two-dimensional or three-dimensional mechanism to move in two-dimensional or three-dimensional space, so that the reagent needle can move to aspirate the reagent carried by the reagent carrying component 13, move to the reaction cup to which the reagent is to be added, and discharge the reagent into the reaction cup.

[0100] The measuring component 30 is used to perform tests on the sample to obtain test data. In some embodiments, the measuring component 30 may include a reaction component 15 and a photometric component 16. Specifically, in the embodiments of this application, the reaction component is a first container and a second container, wherein the first container is used to hold a first mixture prepared from a portion of the sample and a detection reagent; the second container is used to hold a second mixture prepared from another portion of the sample and a diluent. The photometric component 16 is used to perform photometric measurements on the first and second mixtures to obtain the sample's reaction data, such as detecting the luminescence intensity of the reaction solution to be tested and obtaining the concentration of the analyte in the sample by querying a calibration curve. Specifically, in this embodiment, the photometric component 16 includes a multi-wavelength light source, a spectrometer, an optical processing device, and a signal acquisition device. In some embodiments, the photometric component 16 may also be separately disposed outside the reaction component 15.

[0101] based on Figures 1 to 3 The sample analyzer described below, along with the anti-interference measurement method in the embodiments of this application, will be described in detail below. Please refer to [link / reference]. Figure 4 One embodiment of the method for measuring sample interference in this application includes:

[0102] 401. Take the sample to be tested;

[0103] In existing technologies, when detecting the concentration of interfering substances in a sample, a sufficient amount of sample needs to be drawn into a reaction vessel for interfering substance detection, and then a sample is drawn from that reaction vessel and added to another reaction vessel for normal coagulation testing.

[0104] According to the Lambert-Beer law A = ELC, where A represents the absorbance of the interfering substance to a specific wavelength of light that the photometer can measure, E represents the absorption coefficient, L represents the distance that the light of a specific wavelength travels in the sample, and C represents the concentration of the interfering substance in the sample, this method will not be able to accurately measure the concentration of the interfering substance in the sample when the concentration C of the interfering substance in the sample exceeds the detection threshold C1 of the photometer for the concentration of the interfering substance.

[0105] To address this issue, when performing project testing and measuring interfering substances in the sample, the sample analyzer first picks up the sample to be tested, and then performs steps 402 and 403.

[0106] 402. A portion of the sample is added to the first container to prepare a first mixture of the sample and the detection reagent;

[0107] When performing tests on the sample, this embodiment of the application adds a portion of the sample to a first container to prepare a first mixture of the sample and the test reagent.

[0108] 403. Add another portion of the sample to the second container to prepare a second mixture of the sample and diluent;

[0109] When measuring interfering substances in the sample, embodiments of this application add another portion of the sample to a second container to prepare a second mixture of the sample and diluent.

[0110] In this embodiment, the second mixture is a mixture of another part of the sample and the diluent. On the one hand, it ensures that the concentration of interfering substances in the sample can be measured while taking a small amount of sample (i.e. saving the sample). On the other hand, by diluting the sample, the absorbance value detected by the sensor is greatly reduced, which is equivalent to increasing the detection range of the concentration of interfering substances in the sample.

[0111] 404. Irradiate the first mixture and the second mixture using a multi-wavelength light source;

[0112] After obtaining the first mixture and the second mixture, the first mixture and the second mixture are irradiated with a multi-wavelength light source in the optical measurement component, and step 405 is executed.

[0113] 405. Collect the first optical detection signal of the first mixture and the second optical detection signal of the second mixture;

[0114] After irradiating the first and second mixtures with a multi-wavelength light source, the first optical detection signal of the first mixture and the second optical detection signal of the second mixture are collected by the signal acquisition device in the optical measurement component. Based on the second and first optical detection signals, the interfering substances in the samples and the detection items of the samples are analyzed.

[0115] 406. Perform interference analysis based on the second optical detection signal to obtain the interference analysis results of the sample;

[0116] Specifically, the process of performing interference analysis based on the second optical detection signal to obtain the sample interference analysis results will be described in detail in the following embodiments, and will not be repeated here.

[0117] 407. Analyze the detection items of the sample based on the first optical detection signal and the analysis results of the interference, so as to obtain the detection results of the detection items of the sample.

[0118] Similarly, the process of analyzing the sample's detection items based on the first optical detection signal and the analysis results of interference is also described in detail in the following embodiments, and will not be repeated here.

[0119] In this embodiment, a sample to be tested is aspirated; a portion of the sample is added to a first container to prepare a first mixture of the sample and the detection reagent; another portion of the sample is added to a second container to prepare a second mixture of the sample and the diluent; the first mixture and the second mixture are irradiated using a multi-wavelength light source; a first optical detection signal of the first mixture and a second optical detection signal of the second mixture are collected; interference analysis is performed based on the second optical signal to obtain the interference analysis result of the sample; and the detection items of the sample are analyzed based on the first optical detection signal and the interference analysis result to obtain the detection result of the sample. Because this embodiment uses a multi-wavelength light source to irradiate the second mixture prepared from the sample and the diluent, and collects the second optical detection signal of the second mixture to analyze the interference in the sample, it achieves the measurement of interference in the sample while saving sample volume; and by diluting the sample, it significantly reduces the absorbance value detected by the sensor, which is equivalent to increasing the detection range of the concentration of interference in the sample.

[0120] based on Figure 4In the preferred embodiment, the first container is a reaction vessel, the second container is a colorimetric cell, and the second mixture can be obtained by following these steps to achieve a homogeneous second mixture. Please refer to [link to previous text]. Figure 5 , Figure 5 The following are the detailed steps for step 403:

[0121] 501. Add half of the preset total amount of diluent to the colorimetric cell;

[0122] To ensure that the other part of the sample and the diluent are thoroughly mixed, in actual operation, half of the preset total amount of diluent can be added to the colorimetric cell through the diluent addition line located on the side of the colorimetric cell.

[0123] 502. Add another portion of the sample to the colorimetric cell;

[0124] Then another portion of the sample is added to the colorimetric pool.

[0125] 503. Add the other half of the diluent to the colorimetric cell to mix the sample and diluent in the colorimetric cell.

[0126] Finally, add the other half of the diluent to the colorimetric cell, and immediately after adding the sample to the colorimetric cell, use the colorimetric cell addition tube to aspirate and mix the mixture to ensure that the other part of the sample is fully and evenly mixed with the diluent.

[0127] 504. After the measurement is completed, the colorimetric cell shall be cleaned.

[0128] After the measurement is completed, the colorimetric cell is cleaned with diluent. After cleaning, the specified amount of diluent is added and the cell is ready for the next test.

[0129] In this embodiment, a colorimetric cell is used to measure interfering substances in the sample. After the measurement is completed, the colorimetric cell can be cleaned for repeated use. Therefore, this embodiment saves user costs compared to the prior art where a reaction cup is wasted for each sample test. At the same time, the separate cups used in the prior art also affect the testing speed.

[0130] based on Figure 4 In the embodiments described above, when the sample is plasma, the interfering substances include at least one of lipemia, hemolysis, and jaundice, and the multi-wavelength light sources are light sources with wavelengths of 405nm, 545nm, 660nm, and 800nm ​​respectively, the following is a description of... Figure 4 Step 406 in the embodiment is described in detail; please refer to [link / reference]. Figure 6 , Figure 6 The detailed steps of step 406 are as follows:

[0131] 601. Obtain the absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm, respectively;

[0132] Because this embodiment measures the interfering substance by using optical signal acquisition on the diluted sample, it is necessary to obtain the absorbance values ​​of the interfering substance at different wavelengths in the second mixture when calculating the concentration of the interfering substance in the second mixture.

[0133] It should be noted that in the embodiments of this application, the content of the interfering substance is described by the level of the interfering substance. The level of the interfering substance and the concentration of the interfering substance are in one-to-one correspondence, and the correspondence is described in the prior art, so it will not be repeated here.

[0134] For ease of explanation, Figure 7 The absorbance spectra of three different interfering substances are given. Since lipemia (chylous interference) has obvious absorption at 660 nm, while hemoglobin and bilirubin do not show absorption at 660 nm, based on the strong specificity of the absorption spectrum of lipemia, the difference in absorbance at 660 nm and 800 nm is used to determine whether lipemia is present. Therefore, it is necessary to obtain the absorbance values ​​of lipemia at wavelengths of 660 nm and 800 nm respectively.

[0135] 602. The lipid level in the plasma is calculated and analyzed according to the first formula, which is:

[0136]

[0137] Where ΔAbs_L represents the difference in absorbance of lipemia in the second mixture at wavelengths of 660nm and 800nm ​​(relative to the blank channel), C represents the lipemia grade coefficient, and L Index This indicates the level of lipid levels.

[0138] After obtaining the absorbance values ​​of lipemia in the second mixture at wavelengths of 660 nm and 800 nm, the lipemia level in the plasma can be calculated and analyzed according to the first formula.

[0139] 603. Obtain the absorbance values ​​of the hemolyzed solution in the second mixture at wavelengths of 545 nm and 660 nm;

[0140] Because hemolysis (hemoglobin interference) has strong absorption at both 405 and 545 nm, but has strong specificity at 545 nm in the green light band, and jaundice does not show obvious absorption in the green light band, the absorbance of hemolysis at 545 nm in the green light band only includes two cases: lipemia and hemolysis. Subtracting the absorbance of lipemia can yield the absorbance of hemolysis. Therefore, the absorbance values ​​of hemolysis at wavelengths of 545 nm and 660 nm can be obtained separately to calculate the degree of hemolysis.

[0141] 604. The hemolysis level in the plasma is calculated and analyzed according to the second formula, which is:

[0142]

[0143] Where ΔAbs_H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm (relative to the blank channel), A represents the hemolysis grade coefficient, ΔAbs_L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm ​​(relative to the blank channel), E represents the correction coefficient for lipemia when calculating the hemolysis grade, and H represents the hemolysis grade. Index Indicates the degree of hemolysis.

[0144] After obtaining the absorbance values ​​of hemolysis in the second mixture at wavelengths of 545 nm and 660 nm, the hemolysis level in the plasma can be calculated and analyzed according to the second formula.

[0145] 605. Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm;

[0146] Because jaundice (bilirubin interference) has strong absorption around 440nm, this embodiment uses a wavelength of 405nm for testing. Figure 7 As shown in the absorption spectrum, all three types of interference have strong absorption in the 405nm band (jaundice has the weakest anti-interference ability), while the absorption of jaundice is almost zero at 660nm. Therefore, the jaundice level can be calculated by the difference in absorbance at 405nm and 660nm wavelengths. When calculating the jaundice level, the interference of hemolysis and lipemia needs to be subtracted.

[0147] 606. The jaundice level in the plasma is calculated and analyzed according to the third formula, which is:

[0148]

[0149] Wherein, ΔAbs_I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the hemolysis correction factor when calculating jaundice, ΔAbs_H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbs_L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the lipemia correction factor when calculating the hemolysis grade, G represents the lipemia correction factor when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the level of jaundice.

[0150] After obtaining the absorbance values ​​of jaundice at wavelengths of 405nm and 660nm respectively, the jaundice level in plasma can be calculated and analyzed according to the third formula.

[0151] In this embodiment, the process of performing interference analysis based on the second optical detection signal to obtain the interference analysis results of the sample is described in detail, which improves the feasibility of this embodiment.

[0152] based on Figure 4 and Figure 6 The embodiments described above will be further described below. Figure 4 Step 407 in the embodiment is described in detail; please refer to [link / reference]. Figure 8 , Figure 8 Detailed steps for step 407:

[0153] 801. Analyze the detection information corresponding to different wavelengths based on the first optical detection signal;

[0154] When performing multi-wavelength detection analysis on samples (such as immunoturbidimetric analysis), each detection item typically has a dominant wavelength and a secondary wavelength corresponding to the detection item itself. The dominant wavelength and the secondary wavelength used for the detection item each have their own corresponding anti-interference threshold. When performing the detection on the sample, the concentration or level of the interfering substance must be lower than the anti-interference threshold corresponding to the dominant wavelength or the secondary wavelength, otherwise, accurate measurement of the detection item cannot be achieved.

[0155] The interference immunity threshold of the main wavelength refers to the maximum allowable concentration or level of interfering substances when the sample is analyzed using the main wavelength; the interference immunity threshold of the secondary wavelength refers to the maximum allowable concentration or level of interfering substances when the sample is analyzed using the secondary wavelength.

[0156] In this embodiment, the selection of the primary and secondary wavelengths is mainly determined by the reagent requirements in the sample detection process. When performing the detection on the sample, the primary wavelength is required to characterize the reaction process between the sample and the reagent as much as possible, and also to ensure that the signal intensity (i.e. the intensity of the transmitted light) collected by the photometer during the entire test process meets the requirements of the detection.

[0157] When analyzing the detection items of the sample, it is necessary to analyze the detection information corresponding to different wavelengths (i.e., main and secondary wavelengths) together with the interference analysis results in step 802 and the first optical detection signal collected by the signal acquisition device.

[0158] 802. Based on the analysis results of the interference, select the appropriate wavelength and analyze the detection information corresponding to the selected wavelength to obtain the detection results of the detection items of the sample.

[0159] Based on step 801, the appropriate wavelength is selected according to the analysis results of the interfering substances, and the detection information corresponding to the selected wavelength is analyzed to obtain the detection results of the sample's detection items.

[0160] Specifically, when Figure 6 In the embodiment, if the level of the interfering object measured is greater than the anti-interference threshold corresponding to the main wavelength but not greater than the anti-interference threshold corresponding to the secondary wavelength, then the detection information of the main wavelength cannot be used for analysis, and only the detection information of the secondary wavelength can be used for analysis; while when the level of the interfering object is greater than the anti-interference threshold corresponding to the secondary wavelength, then other anti-interference methods must be used to remeasure the detection items of the sample in order to obtain the detection results of the sample detection items.

[0161] In this embodiment of the application, the process of analyzing sample detection items based on the first optical detection signal and the analysis results of the interference is described in detail to obtain the detection results, thereby improving the accuracy of the sample detection results.

[0162] The anti-interference measurement method in this application has been described in detail above. The sample analyzer in this application will be described in detail below. Please refer to [link / reference]. Figure 9 One embodiment of the sample analyzer in this application includes:

[0163] The first container 901 is used to hold the first mixture prepared from a portion of the sample and the detection reagents;

[0164] The second container 902 is used to hold a second mixture prepared from another portion of the sample and the diluent;

[0165] Optical device 903 is used to irradiate the first mixture and the second mixture using a multi-wavelength light source 9031 and an optical processing device 9032.

[0166] The signal acquisition device 904 is used to acquire the first optical detection signal of the first mixture and the second optical detection signal of the second mixture.

[0167] Processor 905 is used to perform interference analysis based on the second optical signal to obtain interference analysis results for the sample;

[0168] The sample is analyzed based on the first optical detection signal and the analysis results of the interference to obtain the detection results of the sample.

[0169] Preferably, the first container is a reaction vessel and the second container is a colorimetric cell;

[0170] The colorimetric cell is specifically used for:

[0171] Add half the preset total amount of diluent;

[0172] Add another portion of the sample;

[0173] Add the other half of the diluent and mix the sample and diluent thoroughly.

[0174] Preferably, the sample analyzer further includes:

[0175] A cleaning device 906 (not shown in the figure) is used to clean the colorimetric cell after the measurement is completed.

[0176] Preferably, the processor 905 is specifically used for:

[0177] Analyze the detection information corresponding to different wavelengths based on the first optical detection signal;

[0178] Based on the analysis results of the interference, a corresponding wavelength is selected, and the detection information corresponding to the selected wavelength is analyzed to obtain the detection results of the detection items of the sample.

[0179] Preferably, the sample is plasma, and the interfering agent includes at least one of lipemia, hemolysis, and jaundice;

[0180] The multi-wavelength light source includes light sources with wavelengths of at least 405nm, 545nm, 660nm and 800nm.

[0181] Preferably, the processor 905 is specifically used for:

[0182] Obtain the absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm;

[0183] The lipid level in the plasma is calculated and analyzed according to the first formula, which is:

[0184]

[0185] Where ΔAbsL represents the difference in absorbance of lipemia in the second mixture at wavelengths of 660 nm and 800 nm, C represents the lipemia grade coefficient, and L Index This indicates the level of lipid levels.

[0186] Preferably, the processor 905 is further configured to:

[0187] Obtain the absorbance values ​​of hemolysis in the second mixture at wavelengths of 545 nm and 660 nm.

[0188] The hemolysis level in the plasma was calculated and analyzed according to the second formula, which is:

[0189]

[0190] Where ΔAbs_H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, A represents the hemolysis grade coefficient, ΔAbs_L represents the absorbance difference of lipohyperfusion in the second mixture at wavelengths of 660nm and 800nm, E represents the correction coefficient for lipohyperfusion when calculating the hemolysis grade, and H represents the hemolysis grade. Index Indicates the degree of hemolysis.

[0191] Preferably, the processor 905 is further configured to:

[0192] Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm.

[0193] The jaundice level in the plasma was calculated and analyzed according to the third formula, which is:

[0194]

[0195] Wherein, ΔAbs_I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the hemolysis correction factor when calculating jaundice, ΔAbs_H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbs_L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the lipemia correction factor when calculating the hemolysis grade, G represents the lipemia correction factor when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the level of jaundice.

[0196] It should be noted that the working principles of the above devices are the same as those of... Figures 4 to 7 The examples described are similar and will not be repeated here.

[0197] In this embodiment, the optical device 903 uses a multi-wavelength light source 9031 and an optical processing device 9032 to irradiate the second mixture prepared from the sample and diluent, and collects the second optical detection signal of the second mixture to analyze the interfering substances in the sample. This achieves the measurement of interfering substances in the sample while saving sample usage. On the other hand, by diluting the sample, the absorbance value detected by the sensor is significantly reduced, which is equivalent to increasing the detection range of the concentration of interfering substances in the sample.

[0198] It should be noted that when the sample analyzer performs coagulation tests, it is a coagulation analyzer. The structure and working principle of the coagulation analyzer can be found in [reference needed]. Figures 4 to 9 The embodiments described herein will not be repeated here.

[0199] This application embodiment also provides a computer-readable storage medium for implementing the functions of a sample analyzer. The medium stores a computer program thereon, which, when executed by a processor, allows the processor to perform the following steps:

[0200] Collect the sample to be tested;

[0201] A portion of the sample is added to the first container to prepare a first mixture of the sample and the detection reagent;

[0202] Another portion of the sample is added to the second container to prepare a second mixture of the sample and diluent;

[0203] The first mixture and the second mixture are irradiated using a multi-wavelength light source;

[0204] Collect the first optical detection signal of the first mixture and the second optical detection signal of the second mixture;

[0205] Interference analysis is performed based on the second optical detection signal to obtain the interference analysis results of the sample;

[0206] The sample is analyzed based on the first optical detection signal and the analysis results of the interference to obtain the detection results of the sample.

[0207] Preferably, the first container is a reaction vessel and the second container is a colorimetric cell. In some embodiments of the present invention, when a computer program stored in a computer-readable storage medium is executed by a processor, the processor may specifically be used to perform the following steps:

[0208] Add half of the preset total amount of diluent to the colorimetric cell;

[0209] Add another portion of the sample to the colorimetric cell;

[0210] Then add the other half of the diluent to the colorimetric cell to mix the sample and the diluent in the colorimetric cell.

[0211] In some embodiments of the present invention, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may also be used to perform the following steps:

[0212] After the measurement is completed, the colorimetric cell is cleaned.

[0213] In some embodiments of the present invention, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically be configured to perform the following steps:

[0214] Analyze the detection information corresponding to different wavelengths based on the first optical detection signal;

[0215] Based on the analysis results of the interference, a corresponding wavelength is selected, and the detection information corresponding to the selected wavelength is analyzed to obtain the detection results of the detection items of the sample.

[0216] Preferably, the sample is plasma, and the interfering agent includes at least one of lipemia, hemolysis, and jaundice. In some embodiments of the present invention, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically be used to perform the following steps:

[0217] The first mixture and the second mixture are irradiated with light sources of wavelengths of 405nm, 545nm, 660nm and 800nm.

[0218] In some embodiments of the present invention, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically be configured to perform the following steps:

[0219] The absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm were obtained respectively.

[0220] The lipid level in the plasma is calculated and analyzed according to the first formula, which is:

[0221]

[0222] Where ΔAbs_L represents the difference in absorbance of lipemia in the second mixture at wavelengths of 660 nm and 800 nm, C represents the lipemia grade coefficient, and L Index This indicates the level of lipid levels.

[0223] In some embodiments of the present invention, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically be configured to perform the following steps:

[0224] Obtain the absorbance values ​​of hemolysis in the second mixture at wavelengths of 545 nm and 660 nm.

[0225] The hemolysis level in the plasma was calculated and analyzed according to the second formula, which is:

[0226]

[0227] Where ΔAbs_H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, A represents the hemolysis grade coefficient, ΔAbs_L represents the absorbance difference of lipohyperfusion in the second mixture at wavelengths of 660nm and 800nm, E represents the correction coefficient for lipohyperfusion when calculating the hemolysis grade, and H represents the hemolysis grade. Index Indicates the degree of hemolysis.

[0228] In some embodiments of the present invention, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically be configured to perform the following steps:

[0229] Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm.

[0230] The jaundice level in the plasma was calculated and analyzed according to the third formula, which is:

[0231]

[0232] Wherein, ΔAbs_I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the hemolysis correction factor when calculating jaundice, ΔAbs_H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbs_L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the lipemia correction factor when calculating the hemolysis grade, G represents the lipemia correction factor when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the level of jaundice.

[0233] Those skilled in the art will clearly understand that, for the sake of convenience and brevity, the specific working processes of the systems, devices, and units described above can be referred to the corresponding processes in the foregoing method embodiments, and will not be repeated here.

[0234] In the several embodiments provided in this application, it should be understood that the disclosed systems, apparatuses, and methods can be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative; for instance, the division of units is only a logical functional division, and in actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or direct coupling or communication connection shown or discussed may be an indirect coupling or communication connection between apparatuses or units through some interfaces, and may be electrical, mechanical, or other forms.

[0235] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.

[0236] Furthermore, the functional units in the various embodiments of the present invention can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The integrated unit can be implemented in hardware or as a software functional unit.

[0237] If the integrated unit is implemented as a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present invention, in essence, or the part that contributes to the prior art, or all or part of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions to cause a computer device (which may be a personal computer, server, or network device, etc.) to execute all or part of the steps of the methods described in the various embodiments of the present invention. The aforementioned storage medium includes various media capable of storing program code, such as USB flash drives, portable hard drives, read-only memory (ROM), random access memory (RAM), magnetic disks, or optical disks.

[0238] The above-described embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An anti-interference measurement method, characterized in that, include: Draw the sample to be tested from the sample tube; A portion of the sample drawn from the sample tube is added to a first container to prepare a first mixture of sample and detection reagent, the first container being a reaction cup; another portion of the sample drawn from the sample tube is added to a second container to prepare a second mixture of sample and diluent, the second container being a colorimetric cell; The first mixture and the second mixture are irradiated using a multi-wavelength light source; and after irradiation, a first optical detection signal of the first mixture and a second optical detection signal of the second mixture are collected respectively. The first optical detection signal includes optical detection signals of different wavelengths among the multi-wavelength light sources, and the second optical detection signal includes optical detection signals of different wavelengths among the multi-wavelength light sources; after the measurement is completed, the colorimetric cell is cleaned. Interference analysis is performed based on the second optical detection signal to obtain the interference analysis results of the sample; The analysis of the detection items of the sample is performed based on the first optical detection signal and the interference analysis results to obtain the detection results of the detection items of the sample. This includes analyzing the first optical detection signal to obtain the detection information corresponding to the optical detection signals of different wavelengths in the multi-wavelength array; selecting the corresponding wavelength from the multi-wavelength array based on the interference analysis results; and analyzing the detection information corresponding to the optical detection signal of the selected wavelength to obtain the detection results of the detection items of the sample.

2. The method according to claim 1, characterized in that, The step of adding another portion of the sample to the second container to prepare a second mixture of the sample and diluent includes: Add half of the preset total amount of diluent to the colorimetric cell; Add another portion of the sample to the colorimetric cell; Then add the other half of the diluent to the colorimetric cell to mix the sample and the diluent in the colorimetric cell.

3. The method according to claim 1, characterized in that, The sample is plasma, and the interfering agent includes at least one of lipemia, hemolysis, and jaundice. The step of irradiating the first mixture and the second mixture using a multi-wavelength light source includes: The first mixture and the second mixture are irradiated with light sources of wavelengths of 405nm, 545nm, 660nm and 800nm.

4. The method according to claim 3, characterized in that, The step of performing interference analysis based on the second optical detection signal to obtain the interference analysis results of the sample includes: The absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm were obtained respectively. The lipid level in the plasma is calculated and analyzed according to the first formula, which is: wherein ΔAbs L represents the absorbance difference of the lipemia in the second mixed solution at 660 nm and 800 nm wavelengths, C represents a lipemia grade coefficient, and L Index represents the lipemia grade.

5. The method according to claim 4, characterized in that, The step of performing interference analysis based on the second optical signal to obtain the interference analysis results of the sample further includes: Obtain the absorbance values ​​of the hemolyzed solution in the second mixture at wavelengths of 545 nm and 660 nm. The hemolysis level in the plasma was calculated and analyzed according to the second formula, which is: Wherein, ΔAbsH represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, A represents the hemolysis grade coefficient, ΔAbsL represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the correction coefficient for lipemia when calculating the hemolysis grade, and H... Index Indicates the degree of hemolysis.

6. The method according to claim 5, characterized in that, The step of performing interference analysis based on the second optical signal to obtain the interference analysis results of the sample further includes: Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm. The jaundice level in the plasma was calculated and analyzed according to the third formula, which is: Wherein, ΔAbs I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the correction factor for hemolysis when calculating jaundice, ΔAbs H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbs L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the correction factor for lipemia when calculating the hemolysis grade, G represents the correction factor for lipemia when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the level of jaundice.

7. A sample analyzer, characterized in that, include: A sample component is used to draw a sample to be tested from a sample tube; a portion of the sample drawn from the sample tube is added to a first container to prepare a first mixture of sample and test reagent, the first container being a reaction cup; Another portion of the sample drawn from the sample tube is added to a second container to prepare a second mixture of the sample and diluent, the second container being a colorimetric cell; A cleaning device is used to clean the colorimetric cell after the measurement is completed; The first container is used to hold the first mixture prepared from a portion of the sample and the detection reagents; The second container is used to hold the second mixture prepared from another portion of the sample and the diluent; An optical device is used to irradiate the first mixture and the second mixture using a multi-wavelength light source; A signal acquisition device is used to acquire a first optical detection signal of the first mixture and a second optical detection signal of the second mixture after the optical device irradiates the first mixture and the second mixture with a multi-wavelength light source, respectively. A processor is configured to perform interference analysis based on the second optical detection signal to obtain interference analysis results for the sample; The analysis of the detection items of the sample is performed based on the first optical detection signal and the interference analysis results to obtain the detection results of the detection items of the sample. This includes analyzing the first optical detection signal to obtain the detection information corresponding to the optical detection signals of different wavelengths in the multi-wavelength array; selecting the corresponding wavelength from the multi-wavelength array based on the interference analysis results; and analyzing the detection information corresponding to the optical detection signal of the selected wavelength to obtain the detection results of the detection items of the sample.

8. The sample analyzer according to claim 7, characterized in that, The colorimetric cell is specifically used for: Add half the preset total amount of diluent; Add another portion of the sample; Add the other half of the diluent and mix the sample and diluent thoroughly.

9. The sample analyzer according to claim 7, characterized in that, The sample is plasma, and the interfering agent includes at least one of lipemia, hemolysis, and jaundice. The multi-wavelength light source includes light sources with wavelengths of at least 405nm, 545nm, 660nm and 800nm.

10. The sample analyzer according to claim 9, characterized in that, The processor is specifically used for: Obtain the absorbance values ​​of the lipids in the second mixture at wavelengths of 660 nm and 800 nm; The lipid level in the plasma is calculated and analyzed according to the first formula, which is: Where ΔAbsL represents the difference in absorbance of lipemia in the second mixture at wavelengths of 660nm and 800nm, C represents the lipemia grade coefficient, and L Index This indicates the level of lipid levels.

11. The sample analyzer according to claim 10, characterized in that, The processor is also used for: Obtain the absorbance values ​​of the hemolyzed solution in the second mixture at wavelengths of 545 nm and 660 nm. The hemolysis level in the plasma was calculated and analyzed according to the second formula, which is: Wherein, ΔAbs H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, A represents the hemolysis grade coefficient, ΔAbs L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the correction coefficient for lipemia when calculating the hemolysis grade, and H Index Indicates the degree of hemolysis.

12. The sample analyzer according to claim 11, characterized in that, The processor is also used for: Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm. The jaundice level in the plasma was calculated and analyzed according to the third formula, which is: Wherein, ΔAbsI represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the hemolysis correction factor when calculating jaundice, ΔAbsH represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbsL represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the lipemia correction factor when calculating the hemolysis grade, G represents the lipemia correction factor when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the level of jaundice.

13. The sample analyzer according to any one of claims 7 to 12, characterized in that, The sample analyzer is a coagulation analyzer.

14. A computer-readable storage medium having a computer program stored thereon, characterized in that, When the computer program is executed by a processor, it is used to implement the anti-interference measurement method as described in any one of claims 1 to 6.

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