A dynamic axial compression chromatography column device and its use method

Abstract

The present invention discloses a dynamic axial compression chromatographic column device, which includes a frame, a chromatographic column and a homogenizing device. The chromatographic column includes a column tube and a lower column cover that can move up and down on the frame. The lower column cover is detachably connected to the lower end of the column tube. A piston assembly that can move up and down is provided inside the column tube. The homogenizing device includes a homogenizing tank that can move on the frame and a homogenizing tank cover that is detachably connected to the homogenizing tank. Its usage method includes the steps: S1, packing is loaded into the homogenizing tank for homogenization, and after the homogenization is completed, it is input into the packing column bed cavity below the piston assembly; S2, the pressure solvent is input into the pressure solvent cavity above the piston assembly through the first pressure solvent inlet, and when the packing pressure reaches the set value, the pressure solvent stops being input; S3, the sample sequentially passes through the mobile phase inlet, the telescopic pipeline, the mobile phase liquid inlet joint and the piston assembly and enters the packing column bed, and after the sample is separated, the solvent is output from the mobile phase outlet. The present invention is convenient to use and can reduce the risk of secondary pollution when unloading the packing.

Description

Technical Field

[0001] The present invention relates to a chromatographic column and a usage method, and particularly to a dynamic axial compression chromatographic column device and a usage method thereof. Background Art

[0002] The existing dynamic axial compression chromatographic column system mainly includes a chromatographic column, a homogenizing device and a piston flipping mechanism. The piston is arranged inside the column tube and is driven by a piston rod to move up and down. The overall structure is complex, the manufacturing cost is high, and the height dimension of the system is large, occupying a large space vertically. In addition, a large amount of manpower is required during the process of disassembling and maintaining the chromatographic column, and the packing is easily secondarily contaminated during the column disassembly process. Summary of the Invention

[0003] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a dynamic axial compression chromatographic column device that is convenient to use and can reduce the risk of secondary contamination when unloading the packing.

[0004] The present invention further provides a usage method of the above-mentioned dynamic axial compression chromatographic column device.

[0005] To solve the above technical problems, the present invention adopts the following technical solutions:

[0006] A dynamic axial compression chromatographic column device includes a frame, a chromatographic column and a homogenizing device provided on the frame. The chromatographic column includes a column tube and a lower cover of the column tube that can move up and down on the frame. The lower cover of the column tube is detachably connected to the lower end of the column tube. A piston assembly that can move up and down is provided inside the column tube. The homogenizing device includes a homogenizing tank that can move on the frame and a cover of the homogenizing tank that is detachably connected to the homogenizing tank.

[0007] As a further improvement of the above technical solution: The chromatographic column further includes an upper cover of the column tube that is detachably connected to the upper end of the column tube. An installation bracket is provided on the frame, and the upper cover of the column tube is provided on the installation bracket. The piston assembly is detachably connected to the upper cover of the column tube.

[0008] As a further improvement of the above technical solution: The piston assembly includes a piston body and a guide sleeve provided on the piston body. An elastic snap ring for clamping the guide sleeve is provided on the lower side of the upper cover of the column tube.

[0009] As a further improvement of the above technical solution: a homogenate outlet is provided on the homogenization tank, a first pressure solvent inlet and a mobile phase inlet are provided on the upper cover of the column tube, a homogenate inlet and a second pressure solvent inlet are provided at the lower end of the column tube, a mobile phase outlet is provided on the lower cover of the column tube, a pressure solvent chamber is formed above the piston assembly, and a packing column bed chamber is formed below the piston assembly. The homogenate outlet and the homogenate inlet are connected through a pipeline. A mobile phase liquid inlet joint is provided on the piston assembly, and the mobile phase inlet and the mobile phase liquid inlet joint are connected through a telescopic pipeline.

[0010] As a further improvement of the above technical solution: the lower cover of the column tube is connected with a lifting driving member. A sliding track and a translation driving member are provided on the frame. A sliding bracket is provided on the sliding track. The homogenization tank and the lifting driving member are arranged on the sliding bracket, and the translation driving member is connected to the sliding bracket.

[0011] As a further improvement of the above technical solution: walking wheels are provided at the bottom of the frame.

[0012] A method for using the above dynamic axial compression chromatography column device includes the following steps.

[0013] S1. Homogenization: The packing is loaded into the homogenization tank for homogenization. After homogenization is completed, the packing is input into the packing column bed chamber below the piston assembly through the homogenate outlet and the homogenate inlet.

[0014] S2. Column packing: The pressure solvent is input into the pressure solvent chamber above the piston assembly through the first pressure solvent inlet. The piston assembly moves downward under the action of the pressure solvent, increasing the pressure of the packing below. When the packing pressure reaches the set value, the input of the pressure solvent stops.

[0015] S3. Sampling: The sample sequentially enters the packing column bed through the mobile phase inlet, the telescopic pipeline, the mobile phase liquid inlet joint and the piston assembly. After the sample is separated, the solvent is output from the mobile phase outlet.

[0016] As a further improvement of the above technical solution: it further includes step S4. Disassembly and maintenance of the lower cover of the column tube: Disconnect the connection between the lower cover of the column tube and the column tube, and the connection between the homogenization tank and the homogenization tank cover. The lifting driving member drives the lower cover of the column tube to descend. After the lower cover of the column tube descends in place, the translation driving member drives the homogenization tank and the lower cover of the column tube to translate until the homogenization tank moves below the column tube. At this time, the lower cover of the column tube is located in the maintenance area and can be cleaned and maintained.

[0017] As a further improvement of the above technical solution: It further includes step S5, packing disassembly and maintenance: Pressure solvent is input into the pressure solvent chamber through the first pressure solvent inlet. The piston assembly moves downward under the action of the pressure solvent, pushing the packing column bed out into the homogenizing tank. After all the packing falls into the homogenizing tank, the input of the pressure solvent stops. The translation driving member drives the homogenizing tank and the lower cover of the column tube back to the initial position, and at this time, the packing can be cleaned and maintained.

[0018] As a further improvement of the above technical solution: It further includes step S6, piston assembly and column tube cleaning and maintenance:

[0019] S6.1. The lifting driving member drives the lower cover of the column tube to rise. After rising in place, the lower cover of the column tube is connected and fixed to the column tube.

[0020] S6.2. Pressure solvent is input into the packing column bed chamber through the second pressure solvent inlet. The piston assembly moves upward under the action of the pressure solvent until the guide sleeve is clamped onto the elastic snap ring.

[0021] S6.3. Disconnect the connection between the upper cover of the column tube and the column tube, and release the pressure solvent in the packing column bed chamber. The lifting driving member drives the column tube and the lower cover of the column tube to descend until the piston body is completely separated from the column tube.

[0022] S6.4. The translation driving member drives the column tube and the lower cover of the column tube to translate to the maintenance area, and at this time, the column tube and the piston assembly can be cleaned and maintained.

[0023] Compared with the prior art, the advantages of the present invention are as follows: The dynamic axial compression chromatography column device disclosed by the present invention has a detachable connection between the lower cover of the column tube and the column tube in the chromatography column. After the lower cover of the column tube is separated from the column tube, it can be lifted and lowered on the frame. The homogenizing tank cover and the homogenizing tank in the homogenizing device have a detachable connection. After the homogenizing tank is separated from the homogenizing tank cover, it can be moved on the frame. When unloading the packing, after the lower cover of the column tube is separated from the column tube, it moves downward to make room under the column tube. After the homogenizing tank is separated from the homogenizing tank cover, it moves to below the column tube. The piston assembly in the column tube moves downward, pushing the packing column bed out of the column tube and falling into the homogenizing tank. It is convenient to use, can reduce manual participation, and reduce the risk of secondary contamination of the packing column bed.

[0024] The use method of the above dynamic axial compression chromatography column device disclosed by the present invention can realize the automatic homogenizing, column packing and sample loading, with a high degree of automation and reduced manual participation. Brief Description of the Drawings

[0025] Figure 1 It is a schematic three-dimensional structure diagram of the dynamic axial compression chromatography column device of the present invention.

[0026] Figure 2 It is a schematic front view structure diagram of the dynamic axial compression chromatography column device of the present invention.

[0027] Figure 3 It is a left view structural schematic diagram of the dynamic axial compression chromatographic column device of the present invention.

[0028] Figure 4 It is a right view structural schematic diagram of the dynamic axial compression chromatographic column device of the present invention.

[0029] Figure 5 It is a structural schematic diagram when the piston assembly moves downward in the usage method of the present invention.

[0030] Figure 6 It is a structural schematic diagram when the lower cover of the column tube moves downward in the usage method of the present invention.

[0031] Figure 7 It is a structural schematic diagram after the homogenizing tank and the lower cover of the column tube are translated in the usage method of the present invention.

[0032] Figure 8 It is a structural schematic diagram after the homogenizing tank and the lower cover of the column tube are reset in the usage method of the present invention.

[0033] Figure 9 It is a structural schematic diagram after the piston assembly is separated from the column tube in the usage method of the present invention.

[0034] Figure 10 It is a structural schematic diagram when the column tube is translated to the maintenance area in the usage method of the present invention.

[0035] In the figure, each label represents: 1, chromatographic column; 11, homogenate inlet; 12, first pressure solvent inlet; 13, mobile phase inlet; 14, second pressure solvent inlet; 15, mobile phase outlet; 16, column tube; 17, lower cover of column tube; 18, lifting drive member; 19, upper cover of column tube; 2, homogenizing device; 21, homogenate outlet; 22, homogenizing tank; 23, lid of homogenizing tank; 24, translation drive member; 25, stirring paddle; 26, pneumatic motor; 27, pneumatic diaphragm pump; 3, piston assembly; 31, piston body; 32, guide sleeve; 33, mobile phase liquid inlet joint; 34, pressure solvent chamber; 35, packing column bed chamber; 4, telescopic pipeline; 5, elastic snap ring; 10, frame; 20, sliding track; 30, sliding bracket; 40, mounting bracket; 50, walking wheel. Detailed implementation manners

[0036] The present invention will be further described in detail below in conjunction with the specification drawings and specific embodiments.

[0037] Figures 1 to 4An embodiment of the dynamic axial compression chromatography column device of the present invention is shown. The dynamic axial compression chromatography column device of this embodiment includes a frame 10, a chromatography column 1 and a homogenizing device 2 provided on the frame 10. The chromatography column 1 includes a column tube 16 and a lower column tube cover 17 that can move up and down on the frame 10. The lower column tube cover 17 is detachably connected to the lower end of the column tube 16. A piston assembly 3 that can move up and down is provided inside the column tube 16. The homogenizing device 2 includes a homogenizing tank 22 that can move on the frame 10 and a homogenizing tank cover 23 that is detachably connected to the homogenizing tank 22. Among them, the homogenizing method of the homogenizing device 2 can be, for example, to set a stirring paddle 25 in the homogenizing tank 22, and use a pneumatic motor 26 outside the homogenizing tank 22 to drive the stirring paddle 25 to rotate, so as to stir the packing evenly. After homogenization is completed, the packing can be pumped from the homogenizing tank 22 into the column tube 16 by a pneumatic diaphragm pump 27. Of course, in other embodiments, the homogenizing device 2 can also adopt other existing homogenizing structures and pumping structures, which will not be elaborated here. The detachable connection between components can be realized by common threaded fasteners, buckles, etc., with reliable connection and convenient disassembly.

[0038] In this dynamic axial compression chromatography column device, the lower column tube cover 17 of the chromatography column 1 is detachably connected to the column tube 16. After the lower column tube cover 17 is separated from the column tube 16, it can move up and down on the frame 10. The homogenizing tank cover 23 of the homogenizing device 2 is detachably connected to the homogenizing tank 22. After the homogenizing tank 22 is separated from the homogenizing tank cover 23, it can move on the frame 10. When unloading the packing, after the lower column tube cover 17 is separated from the column tube 16, it moves downward to vacate the space below the column tube 16. After the homogenizing tank 22 is separated from the homogenizing tank cover 23, it moves to below the column tube 16. The piston assembly 3 inside the column tube 16 moves downward, pushing the packing column bed out of the column tube 16 and falling into the homogenizing tank 22. It is convenient to use, can reduce manual participation, and reduce the risk of secondary contamination of the packing column bed.

[0039] Further, in this embodiment, the chromatography column 1 further includes an upper column tube cover 19 that is detachably connected to the upper end of the column tube 16. An installation frame 40 is provided on the frame 10. The upper column tube cover 19 is provided on the installation frame 40. The piston assembly 3 is detachably connected to the upper column tube cover 19. Connect the column tube 16 to the lower column tube cover 17, and connect the piston assembly 3 to the upper column tube cover 19. At the same time, disassemble the connection between the column tube 16 and the upper column tube cover 19. The upper column tube cover 19 is fixed by the installation frame 40, and the piston assembly 3 is fixed to the upper column tube cover 19. Then the column tube 16 descends synchronously with the lower column tube cover 17. When the column tube 16 is separated from the piston assembly 3, the column tube 16 and the piston assembly 3 can be cleaned and maintained. The structure is simple, reliable and convenient to use. Among them, the installation frame 40 preferably adopts a door frame structure, which is convenient for the column tube 16 and the lower column tube cover 17 to move synchronously with the homogenizing tank 22.

[0040] As a preferred embodiment, the piston assembly 3 includes a piston body 31 and a guide sleeve 32 arranged on the piston body 31, and an elastic snap ring 5 for clamping the guide sleeve 32 is arranged on the lower side of the column tube upper cover 19. When the guide sleeve 32 moves upward and is tightly sleeved on the elastic snap ring 5, the connection between the piston assembly 3 and the column tube upper cover 19 can be realized. When the downward force on the piston body 31 gradually increases, the guide sleeve 32 and the elastic snap ring 5 can be separated. The structure is simple and reliable, and the connection and separation between the guide sleeve 32 and the elastic snap ring 5 can be completed automatically without manual participation, which is very convenient to use.

[0041] Furthermore, in this embodiment, a homogenate liquid outlet 21 is provided on the homogenization tank 22, a first pressure solvent inlet 12 and a mobile phase inlet 13 are provided on the column tube upper cover 19, a homogenate liquid inlet 11 and a second pressure solvent inlet 14 are provided at the lower end of the column tube 16, a mobile phase outlet 15 is provided on the column tube lower cover 17, a pressure solvent chamber 34 is formed on the upper side of the piston assembly 3, a packing column bed chamber 35 is formed on the lower side of the piston assembly 3, the homogenate liquid outlet 21 is connected to the homogenate liquid inlet 11 through a pipeline, a mobile phase liquid inlet connector 33 is provided on the piston assembly 3, and the mobile phase inlet 13 and the mobile phase liquid inlet connector 34 are connected through a telescopic pipe 4. As mentioned above, after the filler is homogenized in the homogenization tank 22, the pneumatic diaphragm pump 27 can be used to pump the filler from the homogenate outlet 21 of the homogenization tank 22 and the homogenate inlet 11 at the lower end of the column tube 16 to the filler column bed cavity 35 on the lower side of the piston assembly 3, and then the pressure solvent is transported to the pressure solvent cavity 34 on the upper side of the piston assembly 3 through the first pressure solvent inlet 12. The piston assembly 3 is acted upon by the pressure solvent, separates from the column tube upper cover 19 and moves downward, and the telescopic pipe 4 is stretched. The filler in the filler column bed cavity 35 is subjected to the pressure of the piston assembly 3, and the mobile phase is continuously discharged through the exhaust valve. When the filler pressure reaches the set pressure, the first pressure solvent inlet 12 stops inputting the pressure solvent, and the column loading work is completed. When the chromatographic column 1 is used to separate the sample, the sample enters the filler column bed through the mobile phase inlet 13 on the column tube upper cover 19, the telescopic pipe 4 and the mobile phase liquid inlet connector 33 on the piston assembly 3. After the sample is separated, the solvent is output from the mobile phase outlet 15 on the column tube lower cover 17. When the pressure solvent is delivered to the packed column bed cavity 34 at the lower side of the piston assembly 3 through the second pressure solvent inlet, the piston assembly 3 moves upward under the action of the pressure solvent and can be connected to the column tube upper cover 19. The pressure solvent is input from different interfaces to move the piston assembly 3 up and down, so that the piston assembly 3 does not need to be equipped with a piston rod, which can greatly reduce the height of the chromatographic column 1 and reduce the vertical space occupied. Among them, the telescopic pipe 4 can be, for example, a metal hose, etc., and each joint can be, for example, a commonly used quick-connect joint.

[0042] As a preferred embodiment, a lifting drive member 18 is connected to the lower cover 17 of the column tube. A sliding track 20 and a translation drive member 24 are provided on the frame 10. A sliding bracket 30 is provided on the sliding track 20. The homogenizing tank 22 and the lifting drive member 18 are arranged on the sliding bracket 30. The translation drive member 24 is connected to the sliding bracket 30. The lifting drive member 18 can drive the lower cover 17 of the column tube (or both the lower cover 17 of the column tube and the column tube 16) to lift and lower. When lowering, it can be separated from the column tube 16 and can move with the homogenizing tank 22 to vacate the space below the column tube 16, facilitating the movement of the homogenizing tank 22 to below the column tube 16. When rising, it can lift the lower cover 17 of the column tube, facilitating the connection of the lower cover 17 of the column tube with the column tube 16. The translation drive member 24 can drive the sliding bracket 30, the homogenizing tank 22 arranged on the sliding bracket 30, the lifting drive member 17, and the lower cover 17 of the column tube connected to the lifting drive member 17 (including the column tube 16 when necessary) to translate. When the homogenizing tank 22 moves to below the column tube 16, it can accommodate the falling packing column bed, while the lower cover 17 of the column tube moves to the maintenance area and can be cleaned and maintained, killing two birds with one stone. Of course, in other embodiments, the lower cover 17 of the column tube can also only perform lifting motion without translating with the homogenizing tank 22. As long as the descending height is sufficient, the space below the column tube 16 can still be vacated. The cooperation between the sliding track 20 and the sliding bracket 30 (such as setting sliders, rollers, ball bearings, etc. on the sliding bracket 30) makes the movement of the sliding bracket 30, etc. smoother and the motion accuracy higher. Among them, the lifting drive member 17 and the translation drive member 24 are preferably cylinders, which can share the air source with the pneumatic motor 26 and the pneumatic diaphragm pump 27 and be coordinately controlled. Of course, in other embodiments, a lead screw-nut pair, a gear-rack pair, etc. can also be used to achieve the purpose.

[0043] As a preferred embodiment, the bottom of the frame 10 is provided with traveling wheels 50, which facilitates the overall movement of the equipment and makes it more convenient to use.

[0044] Figures 5 to 10 An embodiment of the usage method of the dynamic axial compression chromatography column equipment of the present invention is shown. The usage method of the dynamic axial compression chromatography column equipment in this embodiment includes the following steps.

[0045] S1. Homogenization: The packing is loaded into the homogenizing tank 22 for homogenization. After the homogenization is completed, the packing is input into the packing column bed cavity 35 below the piston assembly 3 through the homogenizing liquid outlet 21 and the homogenizing liquid inlet 11.

[0046] S2. Column packing: The pressure solvent is input into the pressure solvent cavity 34 above the piston assembly 3 through the first pressure solvent inlet 12. The piston assembly 3 moves downward under the action of the pressure solvent, increasing the pressure of the packing below. When the packing pressure reaches the set value, the input of the pressure solvent stops.

[0047] S3. Sampling: The sample sequentially passes through the mobile phase inlet 13, the telescopic pipeline 4, the mobile phase liquid inlet joint 33 and the piston assembly 3 and enters the packing column bed. After the sample is separated, the solvent is output from the mobile phase outlet 15.

[0048] The usage method of this dynamic axial compression chromatographic column device can realize the automatic homogenization, column packing and sampling, with a high degree of automation and reduced manual participation.

[0049] Furthermore, in this embodiment, the usage method further includes step S4. Disassembly and maintenance of the lower column tube cover 17: Disconnect the connection between the lower column tube cover 17 and the column tube 16, as well as the connection between the homogenization tank 22 and the homogenization tank cover 23. The lifting drive member 18 drives the lower column tube cover 17 to descend. After the lower column tube cover 17 descends in place, the translation drive member 24 drives the homogenization tank 22 and the lower column tube cover 17 to translate until the homogenization tank 22 moves below the column tube 16. With a high degree of automation and no need for manual participation, at this time, the lower column tube cover 17 is located in the maintenance area and can be cleaned and maintained.

[0050] In this embodiment, the usage method further includes step S5. Disassembly and maintenance of the packing: The pressure solvent is input into the pressure solvent chamber 34 through the first pressure solvent inlet 12. The piston assembly 3 moves downward under the action of the pressure solvent and pushes the packing column bed into the homogenization tank 22. After all the packing falls into the homogenization tank 22, the input of the pressure solvent stops. The translation drive member 24 drives the homogenization tank 22 and the lower column tube cover 17 to return to the initial position. With a high degree of automation and no need for manual participation, at this time, the packing can be cleaned and maintained.

[0051] In this embodiment, the usage method further includes step S6. Cleaning and maintenance of the piston assembly 3 and the column tube 16:

[0052] S6.1. The lifting drive member 18 drives the lower column tube cover 17 to rise. After rising in place, the lower column tube cover 17 is connected and fixed to the column tube 16.

[0053] S6.2. The pressure solvent is input into the packing column bed chamber 35 through the second pressure solvent inlet 14. The piston assembly 3 moves upward under the action of the pressure solvent until the guide sleeve 32 is clamped onto the elastic snap ring 5.

[0054] S6.3. Disconnect the connection between the upper column tube cover 19 and the column tube 16, and release the pressure solvent in the packing column bed chamber 35. The lifting drive member 18 drives the column tube 16 and the lower column tube cover 17 to descend until the piston body 31 is completely separated from the column tube 16.

[0055] S6.4. The translation drive member 24 drives the column tube 16 and the lower column tube cover 17 to translate to the maintenance area. With a high degree of automation and no need for manual participation, at this time, the column tube 16 and the piston assembly 3 can be cleaned and maintained.

[0056] Although the present invention has been disclosed above in preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make many possible changes and modifications to the technical solution of the present invention by using the technical content disclosed above, or modify it into equivalent embodiments with equivalent changes. Therefore, any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the technical solution of the present invention shall fall within the scope of protection of the technical solution of the present invention.

Claims

1. A dynamic axial compression chromatographic column device, comprising a frame (10), a chromatographic column (1) and a homogenizing device (2) provided on the frame (10). Characterized in that: The chromatographic column (1) includes a column tube (16) and a lower column tube cover (17) that can move up and down on the frame (10). The lower column tube cover (17) is detachably connected to the lower end of the column tube (16). A piston assembly (3) that can move up and down is provided inside the column tube (16). The homogenizing device (2) includes a homogenizing tank (22) that can move on the frame (10) and a homogenizing tank cover (23) that is detachably connected to the homogenizing tank (22). The chromatographic column (1) further includes an upper column tube cover (19) that is detachably connected to the upper end of the column tube (16). An installation frame (40) is provided on the frame (10), and the upper column tube cover (19) is arranged on the installation frame (40). The piston assembly (3) is detachably connected to the upper column tube cover (19). A homogenizing liquid inlet (11) and a second pressure solvent inlet (14) are provided at the lower end of the column tube (16). The lower column tube cover (17) is connected to a lifting drive member (18). A sliding track (20) and a translation drive member (24) are provided on the frame (10). A sliding bracket (30) is provided on the sliding track (20). The homogenizing tank (22) and the lifting drive member (18) are arranged on the sliding bracket (30). The translation drive member (24) is connected to the sliding bracket (30). A stirring paddle (25) is arranged inside the homogenizing tank (22).

2. The dynamic axial compression chromatographic column device according to claim 1, Characterized in that: The piston assembly (3) includes a piston body (31) and a guide sleeve (32) provided on the piston body (31). An elastic snap ring (5) for clamping the guide sleeve (32) is provided on the lower side of the upper column tube cover (19).

3. The dynamic axial compression chromatographic column device according to claim 1, Characterized in that: A homogenizing liquid outlet (21) is provided on the homogenizing tank (22). A first pressure solvent inlet (12) and a mobile phase inlet (13) are provided on the upper column tube cover (19). A mobile phase outlet (15) is provided on the lower column tube cover (17). A pressure solvent chamber (34) is formed above the piston assembly (3), and a packing column bed chamber (35) is formed below the piston assembly (3). The homogenizing liquid outlet (21) is communicated with the homogenizing liquid inlet (11) through a pipeline. A mobile phase liquid inlet joint (33) is provided on the piston assembly (3). The mobile phase inlet (13) is communicated with the mobile phase liquid inlet joint through a telescopic pipeline (4).

4. The dynamic axial compression chromatographic column device according to claim 1, Characterized in that: Walking wheels (50) are provided at the bottom of the frame (10).

5. A method for using the dynamic axial compression chromatographic column device according to any one of claims 1 to 4, Characterized in that: Comprising the following steps, S1. Homogenization: The packing is placed in the homogenization tank (22) for homogenization. After homogenization, the packing is input into the packing column bed cavity (35) below the piston assembly (3) through the homogenized liquid outlet (21) and the homogenized liquid inlet (11). S2. Column packing: The pressure solvent is input into the pressure solvent cavity (34) above the piston assembly (3) through the first pressure solvent inlet (12). The piston assembly (3) moves downward under the action of the pressure solvent, increasing the pressure of the packing below. When the packing pressure reaches the set value, the input of the pressure solvent stops. S3. Sample loading: The sample sequentially passes through the mobile phase inlet (13), the telescopic pipeline (4), the mobile phase liquid inlet joint (33), and the piston assembly (3) into the packing column bed. After the sample is separated, the solvent is output from the mobile phase outlet (15).

6. The method for using the dynamic axial compression chromatography column device according to claim 5, characterized in that: It further includes step S4. Disassembly and maintenance of the lower column tube cover (17): Disconnect the connection between the lower column tube cover (17) and the column tube (16), and the connection between the homogenization tank (22) and the homogenization tank cover (23). The lifting drive member (18) drives the lower column tube cover (17) to descend. After the lower column tube cover (17) descends in place, the translation drive member (24) drives the homogenization tank (22) and the lower column tube cover (17) to translate until the homogenization tank (22) moves below the column tube (16). At this time, the lower column tube cover (17) is located in the maintenance area for cleaning and maintenance.

7. The method for using the dynamic axial compression chromatography column device according to claim 6, characterized in that: It further includes step S5. Disassembly and maintenance of the packing: The pressure solvent is input into the pressure solvent cavity (34) through the first pressure solvent inlet (12). The piston assembly (3) moves downward under the action of the pressure solvent, pushing the packing column bed into the homogenization tank (22). After all the packing falls into the homogenization tank (22), the input of the pressure solvent stops. The translation drive member (24) drives the homogenization tank (22) and the lower column tube cover (17) to return to the initial position. At this time, the packing can be cleaned and maintained.

8. The method for using the dynamic axial compression chromatography column device according to claim 7, characterized in that: It further includes step S6. Cleaning and maintenance of the piston assembly (3) and the column tube (16): S6.

1. The lifting drive member (18) drives the lower column tube cover (17) to rise. After rising in place, the lower column tube cover (17) is connected and fixed to the column tube (16). S6.

2. The pressure solvent is input into the packing column bed cavity (35) through the second pressure solvent inlet (14). The piston assembly (3) moves upward under the action of the pressure solvent until the guide sleeve (32) is clamped on the elastic snap ring (5). S6.

3. Disconnect the connection between the upper column tube cover (19) and the column tube (16), and release the pressure solvent in the packing column bed cavity (35). The lifting drive member (18) drives the column tube (16) and the lower column tube cover (17) to descend until the piston body (31) is completely separated from the column tube (16). S6.

4. The translation drive member (24) drives the column tube (16) and the lower cover of the column tube (17) to translate to the maintenance area, and at this time, the column tube (16) and the piston assembly (3) can be cleaned and maintained.

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