Folding sample pretreatment device and sample pretreatment method using the same

Abstract

This application relates to a foldable sample pretreatment device, which includes a reaction tube provided with a reaction chamber, a reagent tube provided with a reagent chamber, a connecting member, and a supporting member. The connecting member is fixed to the outer periphery of the open end of the reaction tube facing the reagent tube, wherein the reagent tube is configured to be pivotally connected to the reaction tube through the connecting member. When the reagent tube rotates until the longitudinal axis of the reagent tube is parallel to the longitudinal axis of the reaction tube, the reagent tube is snap-fitted to the connecting member, and the reaction chamber and the reagent chamber form a fluidly connected closed chamber. The supporting member is arranged on the outer surface of the reaction tube and below the connecting member for preventing the reaction tube from sliding into the reagent tube. This application also provides a sample pretreatment method. The foldable sample pretreatment device described herein can minimize manual intervention, ensure that the sample is not contaminated, and has simple operation and is easy to realize automated continuous operation.

Description

Technical Field

[0001] The present invention relates to the technical field of reaction equipment, and particularly relates to a foldable sample pretreatment device and a sample pretreatment method using the same. Background Art

[0002] In addition to being used in the fields of chemistry and life engineering, fluid sample analysis is also widely used in the diagnostic field for analyzing blood and body fluids collected from patients for diagnosis, especially in the fields of nucleic acid extraction, purification, and detection.

[0003] A key in the method of analyzing fluid samples is the pretreatment of fluid samples. Pretreating fluid samples means extracting a required amount of sample before analyzing the fluid sample, and precisely treating the sample with a dilution buffer or lysis solution, etc. in an appropriate ratio, or mixing the sample with reaction reagents in solid or liquid states, or separating and refining the sample using a filler or support.

[0004] Currently, the devices used for fluid sample pretreatment are difficult to perform continuous operations, opening and closing operations, and some even require manual execution of biochemical processes. Given that fluid samples of biological materials to be processed usually may contain infectious pathogens, therefore, manual intervention during the sample pretreatment process should be minimized.

[0005] In addition, existing fluid sample pretreatment devices usually have difficulty in effectively mixing samples, and after the reaction, centrifugation or other methods are required to separate and transfer the samples.

[0006] The inventor disclosed a sample pretreatment device with less manual intervention and good mixing effect in the patent application for invention with the publication number "CN112393960A" and the title "Sample Pretreatment Device and Sample Pretreatment Method Using the Same". An arc-shaped top is provided in the cover of the sample pretreatment device, and the reaction tube is sleeved inside the reagent tube. When using this sample pretreatment device for sample pretreatment, efficient mixing of materials can be achieved by flipping the sample pretreatment device for ultrasonic treatment, while minimizing manual intervention and avoiding contamination as much as possible. However, in further practical applications, it is still found that there are some deficiencies. For example, when adding a fluid sample, the reaction tube and the cover are separated, which easily causes contamination of the ultrasonic surface of the cover. In addition, during the specific use process, the sample pretreatment device needs to be flipped multiple times, and the operation is slightly complicated, especially it is difficult to achieve automated continuous processing of samples.

[0007] Therefore, there is a continuous need in the art to develop a sample pretreatment device with less manual intervention and easy operation. Summary of the Invention

[0008] The object of the present invention is to provide a sample pretreatment device with less manual intervention and easy to realize automatic continuous processing of samples, so as to overcome the defects of the above-mentioned existing technologies.

[0009] Another object of the present application is to provide a sample pretreatment method using the sample pretreatment device.

[0010] In order to achieve the object of the present invention, the following technical solutions are provided in the present application.

[0011] In a first aspect, the present application provides a foldable sample pretreatment device, which is characterized by comprising:

[0012] A reaction tube, in which a reaction chamber is provided. One end of the reaction tube includes a sealed end, and the other end includes an open end and is connected to a reagent tube through a connector. The sealed end of the reaction tube includes a convex surface away from the arc top of the reaction tube.

[0013] A reagent tube, in which a reagent chamber is provided. One end of the reagent tube is connected to the open end of the reaction tube through a connector, and the other end is provided with a liquid injection port for outputting materials. A pierceable seal is provided on the path where the liquid injection port is in fluid communication with the external environment, and the outer diameter of the reaction tube is smaller than the inner diameter of the reagent tube.

[0014] A connector, which is fixed on the outer periphery of the open end of the reaction tube facing the reagent tube. The reagent tube is configured to be pivotally connected to the reaction tube through the connector. When the reagent tube rotates until the longitudinal axis of the reagent tube is parallel to the longitudinal axis of the reaction tube, the reagent tube is snapped onto the connector, and the reaction chamber and the reagent chamber form a fluid-connected closed chamber.

[0015] And a support member, which is provided on the outer surface of the reaction tube and below the connector for preventing the reaction tube from sliding into the reagent tube.

[0016] In an embodiment of the first aspect, the connector includes a tubular body with through holes at both ends, a hinge connected to the tubular body, and a first clamping member fixedly connected to the tubular body.

[0017] The outer periphery of the open end of the reagent tube is connected to the tubular body of the connector through the hinge, and the outer periphery of the open end of the reagent tube includes a second clamping member. When the reagent tube rotates until the longitudinal axis of the reagent tube is parallel to the longitudinal axis of the reaction tube, the first clamping member and the second clamping member cooperate with each other to form a clamping structure.

[0018] In an embodiment of the first aspect, the inner diameter of the tubular body is greater than or equal to the outer diameter of the reaction tube, and the outer diameter of the tubular body is less than or equal to the inner diameter of the opening end of the reagent tube.

[0019] In an embodiment of the first aspect, the first clamping member is an elastic baffle, and the elastic baffle extends a first length outward from the outer surface of the tubular body along the radial direction of the tubular body, and then extends a second length along the longitudinal axis of the tubular body in a direction away from the opening end of the reaction tube;

[0020] The second clamping member is a convex block, and the convex block extends a third length outward from the outer surface of the reagent tube along the radial direction of the reagent tube, and the third length gradually decreases along the direction away from the liquid injection port of the reagent tube;

[0021] Wherein, the maximum value of the third length is greater than the first length.

[0022] In an embodiment of the first aspect, the upper end of the elastic baffle includes a bent portion that inclines outward along the radial direction of the tubular body.

[0023] In an embodiment of the first aspect, the foldable sample pretreatment device further includes a reaction tube seal, and the reaction tube seal is disposed between the annular gap formed by the connecting member and the reaction tube.

[0024] In an embodiment of the first aspect, the foldable sample pretreatment device further includes a connecting member seal, and the connecting member seal is disposed between the annular gap formed between the reagent tube and the tubular body of the connecting member.

[0025] In an embodiment of the first aspect, the end of the reagent tube provided with the liquid injection port includes a tapered portion whose diameter gradually decreases toward the liquid injection port.

[0026] In an embodiment of the first aspect, the foldable sample pretreatment device further includes a protective cover, and the protective cover is detachably fixed to the periphery of the liquid injection port.

[0027] In an embodiment of the first aspect, the foldable sample pretreatment device further includes a liquid injection port seal, and the liquid injection port seal is disposed between the annular gap formed by the liquid injection port and the protective cover.

[0028] In an embodiment of the first aspect, the foldable sample pretreatment device further includes a filtering device, and the filtering device is above the fluid flow path between the reagent chamber and the liquid injection port.

[0029] In a second aspect, the present application provides a sample pretreatment method using the foldable sample pretreatment device as described in the first aspect, characterized in that the method comprises the following steps:

[0030] (1) Unfasten the clamping connection between the reagent tube and the reaction tube, rotate the reagent tube to expose the open end of the reaction tube, and add the sample to be pretreated into the reaction tube;

[0031] (2) Rotate the reagent tube again and engage the reagent tube with the connecting member provided on the reaction tube, and perform ultrasonic treatment on the sealed end of the sample pretreatment device to obtain the pretreated sample.

[0032] In an embodiment of the second aspect, the method further comprises the following steps:

[0033] (3) After performing ultrasonic treatment on the sample pretreatment device, flip the sample pretreatment device so that the reagent tube is below the reaction tube, pierce the pierceable seal provided on the path of the liquid injection port in fluid communication with the external environment, remove the support member, push the reaction tube into the reagent chamber of the reagent tube, and output the pretreated sample.

[0034] Compared with the prior art, the beneficial effect of the present invention is that the foldable sample pretreatment device described herein can minimize manual intervention, ensure that the sample is not contaminated, and has simple operation and is easy to realize automated continuous operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 FIG. is a perspective view of a foldable sample pretreatment device according to an embodiment of the present invention.

[0036] Figure 2 For Figure 1 the front view of the perspective view shown.

[0037] Figure 3 For Figure 2 the schematic cross-sectional structure view of the front view shown.

[0038] Figure 4 FIG. is a perspective view of a foldable sample pretreatment device according to another embodiment of the present invention.

[0039] Figure 5 FIG. is a perspective view of the foldable sample pretreatment device in a folded state according to an embodiment of the present invention.

[0040] Figure 6 For Figure 5 the schematic cross-sectional structure view of the perspective view shown.

[0041] Figure 7Stereogram when transferring a sample by a foldable sample pretreatment device according to an embodiment of the present invention.

[0042] Figure 8 is Figure 7 Schematic cross-sectional structure diagram of the stereogram shown.

[0043] In the above-mentioned drawings, the meanings of the reference numerals are as follows:

[0044] 100 Sample pretreatment device

[0045] 10 Reaction tube

[0046] 101 Reaction chamber

[0047] 102 Arc portion

[0048] 103 Tubular protective wall

[0049] 104 Reaction chamber seal

[0050] 20 Reagent tube

[0051] 201 Reagent chamber

[0052] 202 Filter device

[0053] 203 Liquid injection port

[0054] 204 Pierceable seal

[0055] 205 Liquid injection port sealing ring

[0056] 206 Second clamping member

[0057] 30 Connector

[0058] 301 Tubular body

[0059] 302 Hinge

[0060] 303 First clamping member

[0061] 304 Connector seal

[0062] 40 Support member

[0063] 50 Protective sleeve

[0064] 501 Connecting band. Detailed implementation manners

[0065] Unless otherwise defined, technical terms or scientific terms used in this specification and claims shall have the ordinary meanings understood by those of ordinary skill in the technical field to which the present invention pertains. All numerical values between the lowest value and the highest value listed herein refer to all numerical values obtained by incrementing by one unit between the lowest value and the highest value when the difference between the lowest value and the highest value is more than two units. In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation on the present invention. In addition, the terms "first", "second", etc. are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first", "second", etc. may explicitly or implicitly include one or more of such features. In the description of the present invention, unless otherwise stated, the meaning of "a plurality" is two or more.

[0066] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "mounted", "connected", "coupled" shall be construed broadly. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood through specific situations.

[0067] The specific embodiments of the present invention will be described below with reference to the drawings. It should be noted that during the specific description of these embodiments, for the sake of concise description, this specification cannot describe all features of the actual embodiments in detail. Without departing from the spirit and scope of the present invention, those skilled in the art can modify and replace the embodiments of the present invention, and the obtained embodiments are also within the protection scope of the present invention.

[0068] First, refer to Figure 1 , Figure 1 which shows a perspective view of a foldable sample pretreatment device according to an embodiment of the present invention. In one embodiment, the present application provides a foldable sample pretreatment device 100. As Figure 1As shown, the foldable sample pretreatment device 100 may include a reaction tube 10, a reagent tube 20, a connecting member 30, and a support member 40. In another embodiment, in order to ensure that the sample after pretreatment is not contaminated, the foldable sample pretreatment device 100 may further include a protective sleeve 50 detachably connected to the outer periphery of the liquid injection port 203 of the reagent tube 20.

[0069] Next, refer to Figure 2 and Figure 3 , Figure 2 Show Figure 1 The front view of the perspective view shown. Figure 3 Further show Figure 2 The schematic cross-sectional structure of the front view shown. As Figure 2 and Figure 3 shown, the reaction tube 10 may include a hollow reaction chamber 101, which can provide a space for the reaction of the sample to be pretreated with the reagent, and can provide a temperature control device, a pressure control device, a gas source, etc. according to the actual situation. One end of the reaction tube 10 may be a sealed end, and the sealed end may include an arc-shaped top 102 with a convex surface away from the reaction tube. The arc-shaped top 102 serves as an ultrasonic surface to enhance the ultrasonic effect of the materials in the reaction tube. In order to facilitate the picking and placing of the foldable sample pretreatment device 100 and further improve the ultrasonic effect, the outer periphery of the arc-shaped top 102 may further include a tubular protective wall 103. The inner diameter of the tubular protective wall 103 may be slightly larger than the inner diameter of the main body part of the reaction tube 10, and the length of the tubular protective wall 103 extending along the longitudinal direction of the reaction tube 10 may be greater than the length of the arc-shaped top 102 extending along the longitudinal direction of the reaction tube 10. At the same time, the other end of the reaction tube 10 may be an open end and is connected to the reagent tube 20 through the connecting member 30.

[0070] Still refer to Figure 2 and Figure 3, the foldable sample pretreatment device 100 described in this article further includes a reagent tube 20. In one embodiment, a reagent chamber 201 is provided inside the reagent tube 20 for storing or adding solid or liquid reagents. Those skilled in the art can understand that when it is necessary to pre-store reagents in the reagent chamber 201, a sealed space can be set in the reagent chamber 201. For example, a sealed space can be formed by adding a pierceable seal at any position of the reagent chamber, and the solid or liquid reagents to be pre-stored can be stored in this sealed space. In one embodiment, the reagent tube 20 may further include a filtering device 202, which can be arranged between the reagent chamber 201 and the liquid injection port 203 of the reagent tube 20 for filtering the pretreated samples. In one embodiment, the reagent tube 20 may further include a liquid injection port 203 for outputting materials from the reagent tube. In one embodiment, the reagent tube 20 may further include a pierceable seal 204, which is arranged on the path where the liquid injection port 203 is in fluid communication with the external environment. In a specific embodiment, the pierceable seal 204 can be a sealing film. Only after the sealing film is pierced can the materials in the reagent chamber 201 be smoothly output. When a protective sleeve 50 is further arranged on the outer periphery of the liquid injection port 203, a liquid injection port seal 205 can be arranged between the annular gap formed by the protective sleeve 50 and the liquid injection port 203. In one embodiment, the end of the reagent tube 20 provided with the liquid injection port 203 may include a tapered portion whose diameter gradually decreases towards the liquid injection port. In addition, the outer diameter of the reaction tube 10 is smaller than the inner diameter of the reagent tube 20, so that when it is necessary to output materials, the reaction tube 10 can be pushed into the reagent tube 20.

[0071] Different from the sample pretreatment device disclosed by the inventor in the patent application with the publication number "CN112393960A" and the title "Sample Pretreatment Device and Sample Pretreatment Method Using the Same", the reaction tube 10 and the reagent tube 20 of the foldable sample pretreatment device 100 described in this article are connected by a connecting member 30, rather than sleeving the reaction tubes 10 inside the reagent tube 20. In this article, the connecting member 30 can be fixed on the outer periphery of the open end of the reaction tube 10 facing the reagent tube 20, wherein the reagent tube 20 is configured to be pivotally connected to the reaction tube 10 through the connecting member 30. When the longitudinal axis of the reagent tube 20 rotates to be parallel to the longitudinal axis of the reaction tube, the reagent tube 20 is clamped to the connecting member 30, and the reaction chamber 101 and the reagent chamber 201 form a fluid-connected sealed chamber.

[0072] Next, refer to Figure 5 and Figure 6 , Figure 5 is a perspective view of the foldable sample pretreatment device in the folded state according to an embodiment of the present invention. Figure 6 is Figure 5Schematic cross-sectional structure diagram of the perspective view shown. In the present application, by pivotally connecting the reagent tube 20 to the reaction tube 10, the entire foldable sample pretreatment device 100 can fold the reagent tube 20 and the reaction tube 10 into two independent parts when adding the sample to be pretreated. In this case, the reaction tube 10 and the reagent tube 20 can be fed or other required operations can be performed separately, and then only by rotating the reagent tube 20, the reagent tube 20 can be snapped onto the connecting member 30. Due to the presence of the connecting member 30, it is not necessary to feed from the end of the reaction tube 10 that needs to be ultrasonically treated, so the bottom of the reaction tube 10 can be a sealed end. This not only avoids sample contamination but also reduces the number of flips of the foldable sample pretreatment device 100, making it easy to achieve automated continuous processing of the sample.

[0073] Reference Figure 5 , in a specific embodiment, the connecting member 30 may include a tubular body 301 with through ends at both ends, a hinge 302 connected to the tubular body, and a first clamping member 303 fixedly connected to the tubular body. The outer periphery of the open end of the reagent tube 20 can be connected to the tubular body 301 of the connecting member 30 through the hinge 302, and the outer periphery of the open end of the reagent tube 20 includes a second clamping member 206. When the reagent tube 20 rotates until the longitudinal axis of the reagent tube is parallel to the longitudinal axis of the reaction tube, the first clamping member 303 and the second clamping member 206 cooperate with each other to form a clamping structure. In Figure 5 the embodiment shown, the inner diameter of the tubular body 301 is greater than or equal to the outer diameter of the reaction tube 10, and the outer diameter of the tubular body 301 is less than or equal to the inner diameter of the open end of the reagent tube 10.

[0074] In a specific embodiment, the first clamping member 303 is an elastic baffle. The elastic baffle 302 extends a first length outward from the outer surface of the tubular body 301 along the radial direction of the tubular body 301, and then extends a second length along the longitudinal axis of the tubular body in a direction away from the opening end of the reaction tube. The second clamping member 206 is a bump. The bump extends a third length outward from the outer surface of the reagent tube 20 along the radial direction of the reagent tube, and the third length gradually decreases in a direction away from the liquid injection port of the reagent tube. The maximum value of the third length is greater than the first length. Such a setting enables the bump to be firmly clamped into the elastic baffle. In a preferred embodiment, the upper end of the elastic baffle includes a curved portion that slopes outward along the radial direction of the tubular body, facilitating the operator to unlock the clamping structure. Those skilled in the art can understand that although an embodiment of the clamping structure is shown herein, it is not limited thereto. Any rotational structure that can rotatably connect the reagent tube 20 to the reaction tube 10 and any clamping structure that can fix the reagent tube 20 and the reaction tube 10 can be used in this application as long as it does not prevent the folding and operation of the foldable sample pretreatment device 100.

[0075] In a specific embodiment, the foldable sample pretreatment device 100 described herein further includes a reaction tube seal 104. The reaction tube seal 104 is disposed in the annular gap formed between the connector 30 and the reaction tube 10.

[0076] In a specific embodiment, the foldable sample pretreatment device 100 described herein further includes a connector seal 304. The connector seal 304 is disposed in the annular gap formed between the reagent tube and the tubular body of the connector.

[0077] In a specific embodiment, the foldable sample pretreatment device 100 further includes a protective cover 50. The protective cover 50 can be detachably fixed to the outer periphery of the liquid injection port 203 through a connecting band 501. As Figure 4 shown, the protective cover 50 can be separated from the liquid injection port 203 of the reagent tube 20. When the protective cover 50 needs to be used, it can be connected to the liquid injection port 203 again. In a specific embodiment, the foldable sample pretreatment device further includes a liquid injection port seal 205. The liquid injection port seal 205 is disposed in the annular gap formed between the liquid injection port and the protective cover.

[0078] In a second aspect, the present application provides a sample pretreatment method using the foldable sample pretreatment device 100 described above. In a specific embodiment, the method includes the following steps:

[0079] (1) Unfasten the clamping structure between the reagent tube 20 and the reaction tube 10, rotate the reagent tube 20 to expose the open end of the reaction tube 10, and add the sample to be pretreated into the reaction tube 10;

[0080] (2) Rotate the reagent tube 20 again and engage the reagent tube 20 with the connector 30 provided on the reaction tube 10, and perform ultrasonic treatment on the sealed end of the reaction tube 10 of the sample pretreatment device to obtain a pretreated sample.

[0081] In a specific embodiment, the method further includes the following steps: (3) After performing ultrasonic treatment on the sample pretreatment device, flip the sample pretreatment device so that the reagent tube is below the reaction tube, pierce the pierceable seal provided on the path of the injection port in fluid communication with the external environment, remove the support member, and push the reaction tube into the reagent chamber 201 of the reagent tube to output the pretreated sample.

[0082] The sample pretreatment method described herein will be described in more detail below with reference to the accompanying drawings. The initial state of the foldable sample pretreatment device 100 is as Figures 1-3 shown. At this time, the reaction tube 20 is connected to the reagent tube 10 through the connector 30, and the reaction tube 20 is clamped to the elastic baffle of the connector 30 through the bump. The support member 40 is disposed on the reaction tube 10 against the tubular body 301 of the connector 30, but below the tubular body 301. The support member 30 can prevent the reaction tube 10 from sliding into the reagent tube 20 before use.

[0083] Inside the foldable sample pretreatment device 100, a three-dimensional connected sealed reaction chamber is formed between the reaction chamber 101 and the reagent chamber 201. A reaction tube seal ring is provided between the annular gap between the connector 30 and the reaction tube 10, and a connector seal ring is provided between the annular gap between the connector 30 and the reagent tube 20. In order to cooperate with the connector 30, one end of the reagent tube 20 connected to the reaction tube 10 may include a portion with an inner diameter larger than the inner diameter of the main body portion of the reagent tube 20, and the inner diameter of this portion is larger than the outer diameter of the tubular body of the connector 30, so that the reagent tube 20 can be clamped outside the tubular body 301 of the connector 30. In a specific embodiment, in order to enable the reaction tube 10 to be smoothly pushed into the reagent tube 20, the inner diameter of the tubular body 301 of the connector 30 may be substantially equal to the inner diameter of the main body portion of the reagent tube 20.

[0084] Next, as Figure 5 and 6As shown, when it is necessary to add a sample to be pretreated into the foldable sample pretreatment device 100, the clamping structure between the bump of the reagent tube 20 and the elastic baffle of the connecting member 30 can be first released, and then the reagent tube 20 is rotated counterclockwise to expose the open end of the reaction tube 10 and the open end of the reagent tube 20. The sample to be pretreated is added into the reaction tube 10 respectively, and the required reagent is added into the reagent tube 20. After the sample addition is completed, the reagent tube 20 is rotated clockwise again to make the bump of the reagent tube 20 snap between the elastic baffles of the connecting member 30. The reaction chamber 101 of the reaction tube 10 and the reagent chamber 201 of the reagent tube 20 form a closed and fluid-connected reaction cavity.

[0085] The arc portion of the sealed end of the reaction tube 10 can be ultrasonically treated by using conventional techniques, so that the sample and the reagent in the foldable sample pretreatment device 100 are mixed evenly and react fully.

[0086] After the sample pretreatment is completed, the pretreated sample needs to be output from the foldable sample pretreatment device 100 and transferred to other reaction devices. Refer to Figure 7 and Figure 8 , first turn over the foldable sample pretreatment device 100, remove the support member 40, and then pierce the pierceable seal 204 at the liquid injection port 3. Then push the reaction tube 10 into the reagent tube 20, and the treated sample can be smoothly discharged from the foldable sample pretreatment device 100. In the Figure 7 and Figure 8 shown embodiment, a protective cover 50 detachably connected to the liquid injection port 203 is further provided on the outer periphery of the liquid injection port 203. Those skilled in the art can understand that in this case, the protective cover 50 needs to be removed first and then the sample transfer is carried out.

[0087] The above description of the embodiments is for the convenience of those of ordinary skill in the art to understand and apply the present application. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative efforts. Therefore, the present application is not limited to the embodiments here, and the improvements and modifications made by those skilled in the art according to the content disclosed in the present application are within the scope of the present application without departing from the scope and spirit of the present application.

Claims

1. A foldable sample pretreatment device, characterized in that, it includes: A reaction tube, which is provided with a reaction chamber inside. One end of the reaction tube includes a sealed end, and the other end includes an open end and is connected to a reagent tube through a connector. Wherein the sealed end of the reaction tube includes a convex surface away from the arc top of the reaction tube; A reagent tube, which is provided with a reagent chamber inside. One end of the reagent tube is connected to the open end of the reaction tube through a connector, and the other end is provided with a liquid injection port for outputting materials. And a pierceable seal is provided on the path where the liquid injection port communicates with the external environment. And the outer diameter of the reaction tube is smaller than the inner diameter of the reagent tube; A connector, which is fixed on the outer periphery of the open end of the reaction tube facing the reagent tube. Wherein the reagent tube is configured to be pivotally connected to the reaction tube through the connector. When the reagent tube rotates until the longitudinal axis of the reagent tube is parallel to the longitudinal axis of the reaction tube, the reagent tube is clamped to the connector, the reaction chamber communicates with the reagent chamber, and the reaction tube, the reagent tube and the connector jointly enclose a fluid-connected closed chamber; And a support member, which is arranged on the outer surface of the reaction tube and below the connector, and is used to prevent the reaction tube from sliding into the reagent tube.

2. The foldable sample pretreatment device according to claim 1, characterized in that, The connector includes a tubular body with through holes at both ends, a hinge connected to the tubular body, and a first clamping component fixedly connected to the tubular body; Wherein the outer periphery of the open end of the reagent tube is connected to the tubular body of the connector through the hinge, and the outer periphery of the open end of the reagent tube includes a second clamping component. When the reagent tube rotates until the longitudinal axis of the reagent tube is parallel to the longitudinal axis of the reaction tube, the first clamping component and the second clamping component cooperate with each other to form a clamping structure.

3. The foldable sample pretreatment device according to claim 2, characterized in that, The inner diameter of the tubular body is greater than or equal to the outer diameter of the reaction tube, and the outer diameter of the tubular body is less than or equal to the inner diameter of the open end of the reagent tube.

4. The foldable sample pretreatment device according to claim 2, characterized in that, The first clamping component is an elastic baffle, which extends a first length outward from the outer surface of the tubular body along the radial direction of the tubular body, and then extends a second length along the longitudinal axis of the tubular body in a direction away from the open end of the reaction tube; The second clamping component is a convex block, which extends a third length outward from the outer surface of the reagent tube along the radial direction of the reagent tube, and the third length gradually decreases along the direction away from the liquid injection port of the reagent tube; Wherein, the maximum value of the third length is greater than the first length.

5. The foldable sample pretreatment device according to claim 4, characterized in that, The upper end of the elastic baffle includes a bent portion that inclines outward along the radial direction of the tubular body.

6. The foldable sample pretreatment device according to any one of claims 1-5, characterized in that, it further comprises a reaction tube seal, and the reaction tube seal is arranged between the annular gap formed by the connecting piece and the reaction tube.

7. The foldable sample pretreatment device according to any one of claims 2-5, characterized in that, it further comprises a connecting piece seal, and the connecting piece seal is arranged between the annular gap formed between the reagent tube and the tubular body of the connecting piece.

8. The foldable sample pretreatment device according to any one of claims 1-5, characterized in that, one end of the reagent tube provided with a liquid injection port comprises a conical portion whose diameter gradually decreases towards the liquid injection port; wherein, the foldable sample pretreatment device further comprises a protective sleeve, and the protective sleeve is detachably fixed to the outer periphery of the liquid injection port; wherein, the foldable sample pretreatment device further comprises a liquid injection port seal, and the liquid injection port seal is arranged between the annular gap formed by the liquid injection port and the protective sleeve.

9. The foldable sample pretreatment device according to any one of claims 1-5, characterized in that, it further comprises a filtering device, and the filtering device is above the fluid flow path between the reagent chamber and the liquid injection port.

10. A sample pretreatment method using the foldable sample pretreatment device according to any one of claims 1-9, characterized in that, the method comprises the following steps: (1) Unfasten the clamping connection between the reagent tube and the reaction tube, rotate the reagent tube to expose the open end of the reaction tube, and add the sample to be pretreated into the reaction tube; (2) Rotate the reagent tube again and make the reagent tube clamped to the connecting piece arranged on the reaction tube, and perform ultrasonic treatment on the sealed end of the sample pretreatment device to obtain the pretreated sample.

11. The method according to claim 10, characterized in that, it further comprises the following steps: (3) After performing ultrasonic treatment on the sample pretreatment device, turn over the sample pretreatment device so that the reagent tube is below the reaction tube, pierce the pierceable seal arranged on the path of fluid communication between the liquid injection port and the external environment, remove the support member, push the reaction tube into the reagent chamber of the reagent tube, and output the pretreated sample.

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