A pollution-proof detection device, method and application for rapid detection of nucleic acid amplification

Abstract

The application discloses a pollution-preventing detection device, method and application for rapid detection of nucleic acid amplification, which comprises a detection part and a mixed reaction cabin, and the mixed reaction cabin comprises a reaction cabin main body and a reaction cabin cover which is in sealed connection with the reaction cabin main body and can be detached; a channel for connecting a hybridization liquid containing part and a mixed reaction part is arranged between the reaction cabin cover and the reaction cabin main body; the detection part comprises a detection inner core and a detection box body containing the detection inner core; a transparent observation window is arranged on the detection box body and corresponds to the reaction area position of the detection inner core; a flow guide device matched with the mixed reaction cabin is further arranged on the detection box body; the flow guide device comprises a flow guide pipe and a flow guide part; one end of the flow guide part is connected with the flow guide pipe, and the other end is connected with the detection inner core; the hybridization liquid and the sample mixed liquid are guided to the detection inner core; and the detection inner core displays the final detection result in the area corresponding to the transparent observation window. The application realizes that the liquid in the reaction tube flows out to the designated detection card in a completely closed environment, and has the advantages of good sealing effect and prevention of primer aerosol pollution.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of nucleic acid detection, in particular to a pollution-proof detection device, method and application for rapid detection of nucleic acid amplification products. BACKGROUND

[0002] The novel coronavirus belongs to the coronavirus of beta genus, has an envelope, and the particles are round or oval, often pleomorphic, with a diameter of 60-140nm. It is significantly different from SARSr-CoV and MERSr-CoV based on its characteristics. Current research shows that it has more than 85% homology with bat-SL-CoVZC45. Based on the current epidemiological investigation, the incubation period is 1-14 days, mostly 3-7 days. The clinical manifestations are mostly mild, with fever, dry cough, and fatigue as the main manifestations, but there are also severe patients who develop dyspnea and / or hypoxemia after one week, and severe cases can rapidly progress to acute respiratory distress syndrome, septic shock, refractory metabolic acidosis, and coagulation dysfunction, and multiple organ failure. Therefore, early detection, early treatment, and early isolation can effectively prevent the spread of the disease and reduce the proportion of severe and critical patients.

[0003] Nucleic acid extraction has become the most important and basic operation in molecular biology experimental techniques, but there are many extraction schemes for viral nucleic acid at present, and the complexity varies. Personnel-intensive units at the grassroots level are often the starting point of infectious disease outbreaks, and it is very necessary to quickly diagnose nucleic acid on site, which has great significance for the prevention and control of infectious diseases.

[0004] However, according to the detection level and treatment conditions of the field or grassroots, it is urgent to develop a new crown nucleic acid rapid detection product suitable for community and personal on-site detection to alleviate the detection pressure of medical institutions in areas with serious epidemic situation and reduce the risk of cross-infection of the subjects. SUMMARY

[0005] In order to solve the problems of the prior art, on the one hand, the present application provides a pollution-proof detection device for rapid detection of nucleic acid amplification products, comprising a detection part and a mixing reaction cabin,

[0006] The mixing reaction cabin comprises:

[0007] The reaction cabin body is provided with a hybridization liquid containing part and a mixing reaction part in the reaction cabin body, and the hybridization liquid containing part and the mixing reaction part are respectively provided with cavities containing hybridization liquid and a sample to be detected,

[0008] A reaction chamber cover is detachably connected to the reaction chamber body in a sealed manner, and a channel is arranged between the reaction chamber cover and the reaction chamber body to communicate the hybridization liquid containing part and the mixed reaction part, so that the hybridization liquid and the sample can be mixed and reacted through the channel when the mixed reaction chamber containing the hybridization liquid and the sample is inverted; the reaction chamber cover is further provided with a flow guide port penetrating the reaction chamber cover, and the flow guide port is provided with a pierceable sealing cap;

[0009] The detection part includes a detection inner core and a detection box body containing the detection inner core, and a transparent observation window is arranged on the detection box body at a position corresponding to the reaction area of the detection inner core; the detection box body is further provided with a flow guide device matched with the mixed reaction chamber, and the flow guide device includes:

[0010] A flow guide tube is used to pierce the sealing cap and guide the mixed solution of the hybridization liquid and the sample after the mixed reaction to the flow guide component,

[0011] The flow guide component is connected to the flow guide tube at one end and connected to the detection inner core at the other end, so as to guide the mixed solution of the hybridization liquid and the sample to the detection inner core, and the detection inner core displays the final detection result in the area corresponding to the transparent observation window.

[0012] Further, the detection box body includes an upper shell and a lower shell, and the upper shell and the lower shell are connected in a sealed manner, and a detection cavity containing the detection inner core is formed between the upper shell and the lower shell, and the detection inner core is arranged in the detection cavity;

[0013] An upper surface of the upper shell is provided with the flow guide device.

[0014] Further, the flow guide device further includes a fixing seat and a plug, the flow guide tube is installed in the fixing seat and penetrates the fixing seat, the upper shell is provided with a scratch flow guide hole, the fixing seat is sleeved in the scratch flow guide hole and located outside the detection box body, the plug is fixedly or detachably connected to the fixing seat from the inner side of the upper shell, so as to fixedly connect the flow guide tube to the upper shell, and one end of the flow guide component is installed on the plug and in contact with the flow guide tube.

[0015] Further, the flow guide component is a water guide fiber membrane, and the water guide fiber membrane extends from the lower end of the flow guide tube to the detection inner core.

[0016] Further, the detection inner core is a nucleic acid colloidal gold paper strip.

[0017] Further, the flow guide device is further provided with a full-enclosed or semi-enclosed annular mounting part extending upward from the outer surface of the upper shell, the annular mounting part is matched with the shape and size of the mixed reaction chamber, and the mixed reaction chamber can be clamped and installed into the annular mounting part.

[0018] Further, the breakage guide hole is arranged on the upper shell and located in the annular mounting portion close to the transparent observation window, and a mixed reaction portion fixing hole is arranged on the upper shell and located in the annular mounting portion away from the transparent observation window, the mixed reaction portion is formed by a reaction cabin main body lower surface extending outward to form a reaction zone protruding portion, and the reaction zone protruding portion is matched with the reaction portion fixing hole in size.

[0019] Further, the reaction zone protruding portion is a combination of a cylinder and a cone, and the cavity for containing liquid is also a combination of a cylinder and a cone.

[0020] Further, the guide hole is arranged above the corresponding mixed reaction portion.

[0021] Further, the hybridization liquid containing portion includes a hybridization liquid container which can be disassembled, and a partition plate is arranged in the reaction cabin main body to divide the reaction cabin body into two chambers, one side is a mixed reaction portion for containing samples, and the other side is a hybridization liquid container placement portion for placing a hybridization liquid container.

[0022] Further, the hybridization liquid container is pre-packaged and sealed, and the opening is sealed by a peelable sealing film.

[0023] The application also provides a pollution prevention detection method for rapid detection of nucleic acid amplification products, which uses any of the above-mentioned pollution prevention detection devices for rapid detection of nucleic acid amplification products, and then includes the following steps:

[0024] a) open the reaction cabin cover, add hybridization liquid into the hybridization liquid containing portion, put the PCR probe into the mixed reaction portion, and then add the sample to be detected into the mixed reaction portion;

[0025] b) cover the reaction cabin cover, and perform isothermal amplification on the sample to be detected in the mixed reaction portion;

[0026] c) then invert and tilt the mixed reaction cabin, so that the hybridization liquid and the sample flow to the chamber of the mixed reaction portion corresponding to the guide hole under the action of gravity for mixing reaction, after mixing, the mixed reaction cabin is inverted and placed on the upper shell, and the guide hole is aligned with the guide tube, the guide tube pierces the sealing cap and guides the mixed hybridization liquid and sample after mixing into the guide component, the guide component guides the hybridization liquid and sample mixture to the detection inner core, and the detection inner core displays the final detection result in the area corresponding to the transparent observation window.

[0027] Further, d) is further included, after detection, the pollution prevention detection device for rapid detection of nucleic acid amplification products is not disassembled, and the whole is discarded in a safe place.

[0028] The application also provides application of the anti-pollution detection device for rapid detection of nucleic acid amplification products or the anti-pollution detection method for rapid detection of nucleic acid amplification products in the food industry, agriculture, animal husbandry, customs quarantine, gene mutation detection and DNA single nucleotide polymorphism identification.

[0029] The application has the following beneficial effects:

[0030] 1. The anti-pollution detection device for rapid detection of nucleic acid amplification products can realize liquid outflow from the mixing reaction cabin to the detection inner core in a completely closed environment, and has the advantages of good sealing effect and prevention of primer aerosol pollution.

[0031] 2. The anti-pollution detection device and method for rapid detection of nucleic acid amplification products can prevent pollution in the detection process due to good sealing performance, can realize rapid real-time detection according to the detection and treatment conditions of the field or the grassroots, and has the advantages of convenient operation, simple use and low professional requirement.

[0032] 3. The anti-pollution detection device, method and application for rapid detection of nucleic acid amplification products have the advantages of being suitable for community and personal on-site detection of new crown nucleic acid rapid detection products, relieving the detection pressure of medical institutions in serious epidemic areas, and reducing the cross-infection risk of the subjects. BRIEF DESCRIPTION OF DRAWINGS

[0033] In order to more clearly illustrate the technical solutions in the embodiments of the application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the application, and other drawings can be obtained by those skilled in the art without creative labor.

[0034] Figure 1 is an exploded view of a mixing reaction cabin of the application;

[0035] Figure 2 is a schematic view of the mixing reaction cabin when it is inverted;

[0036] Figure 3 is a schematic view of the mixing reaction cabin when it is upright;

[0037] Figure 4 is an exploded view of a detection part of the application;

[0038] Figure 5 is a schematic view of the application in use;

[0039] Figure 6 is a schematic view of a hybrid liquid container of the application;

[0040] Figure 7 is a schematic diagram of the mixed reaction cabin of the present application after adding hybridization solution and sample to the sample for isothermal amplification;

[0041] Figure 8 is a schematic diagram of the mixed reaction cabin of the present application after adding hybridization solution and sample to the sample for isothermal amplification;

[0042] Figures 8-10 is a schematic diagram of the mixed reaction cabin of the present application after adding hybridization solution and sample to the sample for isothermal amplification;

[0043] Figures 11-12 is a schematic diagram of the mixed reaction cabin of the present application after adding hybridization solution and sample to the sample for isothermal amplification;

[0044] The example shown in the figure is: mixed reaction cabin 1; reaction cabin body 11; reaction cabin cover 12; mixed reaction part 111; hybridization solution container 112; reaction zone protruding part 113; hybridization solution container installation part 114; flow guide port 121; sealing cap 122; upper shell 21; lower shell 22; sealing ring 23; detection inner core 24; fixed card 241; flow guide part 242; transparent observation window 26; observation window sealing card 261; flow guide pipe 27; sample 4; metal bath device 5; hybridization solution 6; convex edge 7 DETAILED DESCRIPTION

[0045] In order to make the purpose, technical scheme and advantages of the present application clearer, the technical scheme in the embodiments of the present application will be described clearly and completely below in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0046] As shown in Figures 1 to 5 A pollution-proof detection device for rapid detection of nucleic acid amplification product, comprising a detection part and a mixed reaction cabin 1,

[0047] The mixed reaction cabin comprises:

[0048] The reaction cabin body 11 is provided with a hybridization solution containing part and a mixed reaction part 111 in the reaction cabin body 11, and the hybridization solution containing part and the mixed reaction part 111 are respectively provided with cavities for containing hybridization solution and samples to be detected,

[0049] A reaction chamber cover 12 is detachably connected to the reaction chamber body 11 in a sealed manner, and a channel is provided between the reaction chamber cover 12 and the reaction chamber body 11 to communicate the hybridization liquid containing part and the mixing reaction part, so that the hybridization liquid and the sample can be mixed by the channel when the mixing reaction chamber 1 containing the hybridization liquid and the sample is inverted; the reaction chamber cover 12 is further provided with a flow guide port 121 penetrating the reaction chamber cover, and the flow guide port 121 is provided with a pierceable sealing cap 122;

[0050] The detection part includes a detection inner core 24 and a detection box body containing the detection inner core 24, and a transparent observation window 26 is provided on the detection box body at a position corresponding to the reaction area of the detection inner core; the detection box body is further provided with a flow guide device matched with the mixing reaction chamber, and the flow guide device includes:

[0051] A flow guide tube 27 is used to pierce the sealing cap 122 and guide the mixed solution of the hybridization liquid and the sample after mixing reaction to the flow guide component 242,

[0052] The flow guide component 242 is connected to the flow guide tube 27 at one end and connected to the detection inner core 24 at the other end, so as to guide the mixed solution of the hybridization liquid and the sample to the detection inner core 24, and the detection inner core 24 displays the final detection result in the area corresponding to the transparent observation window 26.

[0053] As Figure 1 shown, in actual application, in order to facilitate production and assembly, the detection box body includes an upper shell 21 and a lower shell 22, and the upper shell 21 and the lower shell 22 are connected in a sealed manner, Figure 1 and in an example, a sealing ring 23 is arranged between the upper shell 21 and the lower shell 22 to achieve the effect of sealed connection. The upper shell 21 and the lower shell 22 form a detection cavity containing the detection inner core 24, and the detection inner core 24 is arranged in the detection cavity; Figure 1 and in an example, a plurality of upwardly protruding and equal-height support pieces are arranged on the inner bottom surface of the lower shell 22, and the detection inner core 24 is horizontally placed on the support pieces and fixed in the lower shell 22 by a fixing clamp 241. Figure 1 In an example, the flow guide component 242 is L-shaped, the horizontal section of which is in contact with the right end of the detection inner core, and the vertical section of which is in contact with the flow guide tube 27, and the flow guide tube 27 is arranged vertically to facilitate the mixed solution of the hybridization liquid and the sample to be guided to the flow guide component 242 by gravity, and then infiltrated and transmitted to the detection inner core 24, and finally display the detection result on the detection inner core 24 (generally displayed by a color band).

[0054] The upper surface of the upper shell 21 is provided with the flow guide device.

[0055] As Figure 1 and Figure 5As shown, the flow guide device further comprises a fixing seat 271 and a plug 272, the flow guide tube 27 is installed on the fixing seat 271 and penetrates through the fixing seat 271, the upper shell 21 is provided with a scratch flow hole 28, the fixing seat 271 is sleeved in the scratch flow hole 28 and located outside the detection box body, the plug 272 is fixedly or detachably connected with the fixing seat 271 from the inside of the upper shell 21, and the flow guide tube 27 is fixedly connected on the upper shell 21, one end of the flow guide component 242 is installed on the plug and in contact with the flow guide tube 27.

[0056] In some embodiments, the flow guide component 242 is a water guide fiber membrane, which is extended from the lower end of the flow guide tube to the detection inner core. Other materials with good water guide performance can also be used to meet the requirement of guiding the mixed solution of the hybridization solution and the sample into the detection inner core 24 to achieve the detection purpose.

[0057] Among them, the detection inner core 24 can select a nucleic acid colloidal gold paper strip.

[0058] In some embodiments, the flow guide device is further provided with a full-enclosed or semi-enclosed annular mounting part 30 extending upward from the outer surface of the upper shell 21, the annular mounting part 30 matches the outer shape size of the mixing reaction cabin 1, and the mixing reaction cabin 1 can be clamped and installed into the annular mounting part 30. The setting of the annular mounting part 30 can make the mixing reaction cabin 1 stably connected with the flow guide device during the flow guide detection, so as to avoid falling off and causing external pollution. Meanwhile, when the product is not used, the two are connected together, which is convenient for packaging, transportation and management, and avoids the increase of trouble and cost in packaging, transportation and management caused by separation of the two.

[0059] In Figure 1 , Figure 5 , Figures 9-12 In the example shown, the scratch flow hole 28 is arranged on the upper shell 21 and located in the annular mounting part 30 close to one side of the transparent observation window 26, and the other side of the upper shell 21 located in the annular mounting part 30 away from the transparent observation window 26 is provided with a mixing reaction part fixing hole 29, the mixing reaction part 111 is formed by the lower surface of the reaction cabin body 11 extending outwardly and protruding to form a reaction area protruding part 113, and the reaction area protruding part 113 matches the size of the reaction part fixing hole 29. In this way, during the packaging and transportation of the unused product, the mixing reaction cabin 1 is clamped and arranged on the annular mounting part 30, and the reaction area protruding part is just inserted into the reaction part fixing hole 29. The purpose of arranging the reaction part fixing hole 29 is to make the sample separately immersed into the metal bath device for isothermal amplification after the sample is added, and the hybridization solution and the hybridization solution containing part can keep a proper distance from the metal bath device, which meets the detection requirement and is convenient for the mixing reaction operation after the isothermal amplification is completed.

[0060] In the example shown in the above figure, the reaction zone protrusion 113 is a combination of a cylinder and a cone, and the cavity inside the reaction zone protrusion 113 is also a combination of a cylinder and a cone.

[0061] In a specific implementation, the flow guide 121 is arranged above the mixing reaction part 111.

[0062] In a preferred implementation, the hybridization solution accommodating part includes a hybridization solution container 112 that can be attached and detached, and the reaction chamber main body is provided with a partition plate to divide the reaction chamber main body into two chambers, one of which is the mixing reaction part 111 for accommodating samples, and the other of which is the hybridization solution container accommodating part 114 for placing the hybridization solution container 112.

[0063] In this way, the hybridization solution container 112 can be manufactured in a pre-packaged sealed form, and the opening thereof is sealed by a peelable sealing film 1121, as shown in Figure 6 This ensures that the container containing the hybridization solution maintains the necessary cleanliness before detection, ensuring the accuracy of the detection.

[0064] As shown in Figures 6 to 10 The present application also provides a pollution prevention detection method for rapid detection of nucleic acid amplification products, which uses any of the above-mentioned pollution prevention detection devices for rapid detection of nucleic acid amplification products, and then includes the following steps:

[0065] a) Open the reaction chamber cover 12, add hybridization solution to the hybridization solution accommodating part 114 (and more preferably, after peeling off the sealing film 1121, add the hybridization solution to the hybridization solution container 112 as shown in Figure 6 ), and then place the PCR probe into the mixing reaction part 111, and then add the sample to be detected 4 into the mixing reaction part 111 (in the example shown in the figure, into the reaction zone protrusion 113);

[0066] b) Cover the reaction chamber cover 12, and perform isothermal amplification on the sample to be detected in the mixing reaction part, as shown in Figure 7 The sample is immersed in the metal bath device 5 for isothermal amplification, and the hybridization solution 6 and the hybridization solution accommodating part can be kept at a proper distance from the metal bath device 5, which meets the detection requirements and facilitates the mixing reaction operation after isothermal amplification is completed. Figure 7 In order to ensure this safety distance, a protruding rim 7 can be provided at the bottom of the reaction chamber main body 11.

[0067] c) As shown in Figure 8As shown, then the mixed reaction cabin 1 is inverted and inclined, so that the hybridization solution 6 and the sample 4 flow to the chamber of the mixed reaction part 111 corresponding to the flow guide port 121 under the action of gravity for mixing reaction, after mixing, the mixed reaction cabin 1 is inverted and placed on the upper shell 21, and the flow guide port 121 is aligned with the flow guide pipe 27, the flow guide pipe 27 pierces the sealing cap 122 and guides the mixed hybridization solution 6 and the sample 4 into the flow guide part 242, the flow guide part 242 guides the hybridization solution 5 and the sample 4 to the detection inner core 24, and the detection inner core 24 displays the final detection result in the area corresponding to the transparent observation window 26.

[0068] Further, d) is further included, after detection, without disassembling the anti-pollution detection device for rapid detection of nucleic acid amplification products, the whole is discarded in safety.

[0069] The application also proposes the application of the above-mentioned any anti-pollution detection device for rapid detection of nucleic acid amplification products or the above-mentioned any anti-pollution detection method for rapid detection of nucleic acid amplification products in the food industry, agriculture, animal husbandry, customs quarantine, gene mutation detection and DNA single nucleotide polymorphism identification.

[0070] The application has the following beneficial effects:

[0071] 1、The anti-pollution detection device for rapid detection of nucleic acid amplification products realizes that the liquid in the mixed reaction cabin flows out to the detection inner core in a completely closed environment, and has the advantages of good sealing effect and prevention of primer aerosol pollution;

[0072] 2、The anti-pollution detection device for rapid detection of nucleic acid amplification products and the method can prevent pollution during detection due to good sealing performance, can realize rapid real-time detection according to the detection and treatment conditions of the field or the grassroots, and are convenient to operate, simple to use and low in professional requirement;

[0073] 3、The anti-pollution detection device for rapid detection of nucleic acid amplification products, the method and the application have the advantages of being suitable for community and personal on-site detection of new crown nucleic acid rapid detection products, relieving the detection pressure of medical institutions in serious epidemic areas, and reducing the cross-infection risk of the subjects.

[0074] Common pathogens include bacteria, fungi, viruses, mycoplasma, chlamydia, parasites and the like.

[0075] The closed nucleic acid amplification detection device can not only identify these pathogens, but also identify their subtypes, toxic strains, mutant strains and drug resistance and the like.

[0076] All the above optional technical solutions can be combined to form optional embodiments of the application, which will not be described here.

[0077] The above description is only the preferred embodiment of the present application, and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A contamination-preventing detection device for rapid detection of nucleic acid amplification products, characterized by, The detection device comprises a detection part and a mixed reaction chamber, The mixed reaction chamber comprises: a reaction chamber body, which is provided with a hybrid liquid containing part and a mixed reaction part inside, and the hybrid liquid containing part and the mixed reaction part are respectively provided with cavities containing hybrid liquid and samples to be detected, a reaction chamber cover, which is sealingly connected with the reaction chamber body and can be detached, and a channel is provided between the reaction chamber cover and the reaction chamber body to communicate the hybrid liquid containing part and the mixed reaction part, when the mixed reaction chamber containing the hybrid liquid and the sample is inverted, the hybrid liquid and the sample are mixed through the channel; the reaction chamber cover is also provided with a flow guide port penetrating through the reaction chamber cover, and the flow guide port is provided with a pierceable sealing cap; The detection part comprises a detection core and a detection box body containing the detection core, and a transparent observation window is provided on the detection box body corresponding to the reaction area of the detection core, and the detection box body is also provided with a flow guide device matched with the mixed reaction chamber, and the flow guide device comprises: a flow guide pipe for piercing the sealing cap and guiding the mixed hybrid liquid and sample after the mixed reaction to the flow guide part, a flow guide part connected with the flow guide pipe at one end and connected with the detection core at the other end, guiding the hybrid liquid and sample mixture to the detection core, and the detection core displays the final detection result in the area corresponding to the transparent observation window.

2. The anti-pollution detection device for rapid detection of nucleic acid amplification product according to claim 1, wherein The detection box body comprises an upper shell and a lower shell, and the upper shell and the lower shell are sealingly connected to form a detection cavity containing the detection core, and the detection core is arranged in the detection cavity; The upper surface of the upper shell is provided with the flow guide device.

3. The anti-pollution detection device for rapid detection of nucleic acid amplification product according to claim 1, wherein The flow guide device further comprises a fixed seat and a plug, the flow guide pipe is installed in the fixed seat and penetrates through the fixed seat, the upper shell is provided with a scratch flow guide hole, the fixed seat is sleeved in the scratch flow guide hole and located outside the detection box body, the plug is fixedly or detachably connected with the fixed seat from the inside of the upper shell, the flow guide pipe is fixedly connected on the upper shell, and the flow guide part is installed on the plug and in contact with the flow guide pipe.

4. The anti-pollution detection device for rapid detection of nucleic acid amplification product according to claim 3, wherein The flow guide part is a water guide fiber membrane, and the water guide fiber membrane extends from the lower end of the flow guide pipe to the detection core.

5. The contamination-proof detection device for rapid detection of nucleic acid amplification products according to claim 1, characterized by The detection core is a nucleic acid colloidal gold paper strip.

6. The contamination-proof testing device for rapid detection of nucleic acid amplification according to claim 3, characterized by The flow guide device is also provided with a fully or semi-enclosed annular mounting part extending upward from the outer surface of the upper shell, the annular mounting part matches the shape and size of the mixed reaction chamber, and the mixed reaction chamber can be clamped and installed in the annular mounting part.

7. The contamination-proof detection device for rapid detection of nucleic acid amplification according to claim 6, characterized by The scratch flow guide hole is arranged on the upper shell and located in the annular mounting part close to the transparent observation window, and the other side of the upper shell located in the annular mounting part away from the transparent observation window is provided with a mixed reaction part fixing hole, the mixed reaction part is formed by the reaction chamber body extending outward from the lower surface and protruding to form a reaction area protruding part, and the size of the reaction area protruding part matches the size of the reaction part fixing hole.

8. The contamination-proof detection device for rapid detection of nucleic acid amplification according to claim 7, characterized by, The reaction zone protrusion is a combination of a cylinder and a cone, and the cavity for containing liquid inside is also a combination of a cylinder and a cone.

9. The contamination-proof testing device for rapid detection of nucleic acid amplification according to claim 1, characterized by, The flow guide is arranged above the mixing reaction part.

10. The contamination-proof testing device for rapid detection of nucleic acid amplification product according to any one of claims 1 to 9, characterized by, The hybridization solution containing part comprises a detachable hybridization solution container, and the reaction chamber body is provided with a partition plate to divide the reaction chamber body into two chambers, one of which is a mixing reaction part for containing samples, and the other is a hybridization solution container placement part for placing the hybridization solution container.

11. The contamination-proof assay device for rapid detection of nucleic acid amplification according to claim 10, characterized by, The hybridization solution container is pre-packaged and sealed, and the opening is sealed by a peelable sealing film.

12. A method for preventing contamination in rapid detection of nucleic acid amplification products, characterized by, The pollution-preventing detection device for rapid detection of nucleic acid amplification product according to any one of claims 1-11 is used, and the following steps are included: a) open the reaction chamber cover, add hybridization solution into the hybridization solution containing part, put PCR probes into the mixing reaction part, and then add the sample to be detected into the mixing reaction part; b) cover the reaction chamber cover, and perform isothermal amplification on the sample to be detected in the mixing reaction part; c) then invert and tilt the mixing reaction chamber so that the hybridization solution and the sample flow to the chamber of the mixing reaction part corresponding to the flow guide under the action of gravity for mixing reaction, after mixing, place the mixing reaction chamber upside down on the upper shell, and align the flow guide with the flow guide tube, the flow guide tube pierces the sealing cap and guides the mixed hybridization solution and sample mixture into the flow guide part, the flow guide part guides the hybridization solution and sample mixture to the detection inner core, and the detection inner core displays the final detection result in the area corresponding to the transparent observation window.

13. The contamination-proof detection method for rapid detection of nucleic acid amplification according to claim 12, wherein, d) after detection, do not disassemble the pollution-preventing detection device for rapid detection of nucleic acid amplification product, and discard the whole device in a safe place.

14. Use of the pollution-preventing detection device for rapid detection of nucleic acid amplification product according to any one of claims 1-11 or the pollution-preventing detection method for rapid detection of nucleic acid amplification product according to any one of claims 12 or 13 in the food industry, agriculture, animal husbandry, customs quarantine, gene mutation detection, and DNA single nucleotide polymorphism identification.

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