A micro temperature control device and an inkjet biological 3D printer

Abstract

The present invention provides a micro temperature control device and an inkjet biological 3D printer, which belongs to the field of biological 3D printing technology. The micro temperature control device includes a cover body, a connection structure and a base connected in sequence from top to bottom. The cover body and the base both include a refrigeration structure, which is suitable for providing a low-temperature environment for printing and forming biological materials. The two ends of the connection structure are detachably connected to the cover body and the base respectively. The connection structure includes one or more connection modules, and the multiple connection modules are stacked vertically, and the number of the connection modules is adapted to the printing height of the biological material. The present invention realizes the refrigeration of the air near the cover body and the base at the same time by arranging the refrigeration structure in the cover body and the base, which can greatly reduce the temperature gradient inside the entire device, make the temperature inside the entire device tend to be uniform, which is conducive to inkjet biological 3D printing, and the number of the connection modules is adapted to the printing height of the biological material, and the application range is wider.

Description

Technical Field

[0001] The present invention relates to the technical field of biological 3D printing, and in particular to a micro temperature control device and an inkjet biological 3D printer. Background Art

[0002] With the rapid development of human society, people's living standards are also improving, but work pressure is also increasing, and there are more and more patients with cardiovascular diseases, which leads to a shortage of transplantable organs. In such an environment, biomedical engineering has gradually attracted people's attention, and biological 3D printing technology has developed rapidly. Biological 3D printing mainly adopts extrusion and inkjet printing. Due to the limitations of the equipment structure and materials used in extrusion printing, problems such as broken wires and cell damage are prone to occur during the extrusion process. In the process of inkjet biological 3D printing, some biological inks used have temperature-sensitive properties, so temperature control is particularly important during the printing process. In the prior art, the temperature control device of the biological 3D printer is prone to temperature gradients in the air due to the setting of local refrigeration equipment, which makes the internal temperature distribution uneven, affecting the printing effect. Summary of the invention

[0003] The problem solved by the present invention is that in the prior art, due to the setting of a local refrigeration device, the temperature control device of a biological 3D printer easily causes a temperature gradient in the air inside, resulting in uneven temperature distribution inside, which affects at least one aspect of the printing effect.

[0004] To solve the above problems, the present invention provides a micro temperature control device, comprising a cover body, a connecting structure and a base connected in sequence from top to bottom, the cover body and the base both comprising a refrigeration structure, the refrigeration structure being suitable for providing a low-temperature environment for printing and forming biological materials, the two ends of the connecting structure being detachably connected to the cover body and the base respectively, the connecting structure comprising one or more connecting modules, a plurality of the connecting modules being vertically stacked, and the number of the connecting modules being adapted to the printing height of the biological material.

[0005] Preferably, the refrigeration structure includes a first refrigeration structure arranged on the cover body, the first refrigeration structure includes a first cooling element, a first semiconductor cooling element and a cover plate, the first cooling element is detachably connected to the cover plate, and the first semiconductor cooling element is embedded between the first cooling element and the cover plate.

[0006] Preferably, a first protrusion structure cooperating with the connecting structure is provided on the bottom of the cover plate, extending in a direction close to the connecting structure.

[0007] Preferably, the connection module includes two first connection members arranged opposite to each other and a second connection member arranged between the two first connection members, the second connection member is used for placing the observation lens, and the first connection member and the second connection member are detachably connected.

[0008] Preferably, a first positioning groove is provided at the top end of the first connecting member and the second connecting member, and a second protrusion structure is provided at the bottom end of the first connecting member and the second connecting member. The adjacent first connecting member and the second connecting member are detachably connected, and the first positioning groove cooperates with the first protrusion structure or the first positioning groove cooperates with the second protrusion structure of the first connecting member and the second connecting member adjacent to each other in the vertical direction, and the second protrusion structure of the first connecting member and the second connecting member close to the base in the vertical direction is also cooperated with the base.

[0009] Preferably, the connection module comprises a heat preservation sleeve, the first connection member and the second connection member are connected to each other to form a connection body, and the heat preservation sleeve is arranged around the peripheral wall of the connection body.

[0010] Preferably, the vertical stacking of the plurality of connection modules comprises a plurality of connection bodies being arranged in sequence in the vertical direction, and positions of adjacent connection bodies in the vertical direction correspond to each other.

[0011] Preferably, the refrigeration structure includes a second refrigeration structure arranged on the base, the second refrigeration structure also includes a second cooling element, a second semiconductor refrigeration element and a bottom plate, the second cooling element is detachably connected to the bottom plate, and the second semiconductor refrigeration element is embedded between the second cooling element (36) and the bottom plate, and the bottom plate is provided with a second positioning groove that cooperates with the second protruding structure near the top of the connecting structure.

[0012] Preferably, the base also includes an insulation structure, which includes a first insulation component arranged on the side wall of the second refrigeration structure and a second insulation component arranged on the top wall of the second refrigeration structure, and the first insulation component and the second insulation component are both detachably connected to the bottom plate.

[0013] Compared with the prior art, the micro temperature control device of the present invention, on the one hand, realizes the cooling of the air near the cover and the base by setting a cooling structure in the cover and the base. It can greatly reduce the temperature gradient inside the whole device, make the temperature inside the whole device tend to be uniform, which is more conducive to inkjet biological 3D printing and improves the printing effect. On the other hand, the number of connection modules is adapted to the printing height of the biological material, so that the vertical height of multiple connection modules is suitable for the printing and forming of biological products, and the application range is wider.

[0014] In order to solve the above technical problems, the present invention also provides an inkjet biological 3D printer, including an infrared observation device and the micro temperature control device.

[0015] The inkjet biological 3D printer described in the present invention has the same advantages as the micro temperature control device over the prior art, which will not be repeated here. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of a micro temperature control device in an embodiment of the present invention;

[0017] Figure 2 A half-section view of a micro temperature control device in an embodiment of the present invention;

[0018] Figure 3 It is a schematic diagram of the structure of the heat preservation cover in an embodiment of the present invention;

[0019] Figure 4 The structure of the cover body in the embodiment of the present invention is shown in FIG. Figure 1 (without insulation cover);

[0020] Figure 5 The structure of the cover body in the embodiment of the present invention is shown in FIG. Figure 2 (without insulation cover);

[0021] Figure 6 A schematic diagram of the structure of the connection structure in an embodiment of the present invention;

[0022] Figure 7 It is a schematic diagram of the structure of the thermal insulation cover in an embodiment of the present invention;

[0023] Figure 8 is a schematic structural diagram of a second connecting member in an embodiment of the present invention;

[0024] Fig. 9 It is a structural schematic diagram of a base in an embodiment of the present invention;

[0025] Fig.10 The schematic diagram of the partial structure of the base in the embodiment of the present invention is as follows Figure 1 ;

[0026] Fig.11 The schematic diagram of the partial structure of the base in the embodiment of the present invention is as follows Figure 2 .

[0027] Description of reference numerals:

[0028] 1-cover body, 11-insulation cover, 12-first cooling element, 13-first bolt, 14-first fixing element, 15-insulation side plate, 16-insulation top plate, 17-first semiconductor refrigeration element, 18-cover plate, 19-first protruding structure; 2-connecting structure, 21-insulation sleeve, 22-first connecting element, 23-second connecting element, 24-first positioning groove; 3-base, 31-first insulation element, 32-second insulation element, 33-third bolt, 34-second positioning groove, 35-bottom plate, 36-second cooling element, 37-second bolt, 38-second fixing element, 39-second semiconductor refrigeration element. DETAILED DESCRIPTION

[0029] There are many types of existing biological 3D printers, such as extrusion, inkjet, and light-curing. The biological ink used in inkjet biological 3D printers is mostly temperature-sensitive materials such as Gelatin and GelMA. Because they are in a liquid state above the gel temperature and in a gel state below the gel temperature, and because the temperature inside the nozzle is above the gel temperature, the internal biological ink is in a liquid state. Therefore, the nozzle of the inkjet biological 3D printer uses piezoelectric or thermal bubble driving methods to produce tiny droplets at the nozzle mouth. The droplets are quickly ejected and pass through the gas temperature field set in the temperature control device. After the droplets and the gas temperature complete convection heat exchange, their state changes to a gel state and adheres to the bottom plate of the temperature control device. In the process of biological 3D printing, some of the biological inks used have temperature-sensitive properties, so temperature control is particularly important during the printing process. However, in the prior art, the temperature control device of the biological 3D printer is prone to temperature gradients in the air due to the setting of local refrigeration equipment, which makes the internal temperature distribution uneven, affecting the printing effect.

[0030] The technical solutions in the embodiments of the present application will be described clearly and in detail below in conjunction with the accompanying drawings. In the description of the present invention, it should be understood that the positive direction of "X" in the accompanying drawings represents the right, the reverse direction of "X" represents the left, the positive direction of "Y" represents the top, the reverse direction of "Y" represents the bottom, the positive direction of "Z" represents the front, and the reverse direction of "Z" represents the back, and the orientation or position relationship indicated by the terms "X", "Y" and "Z" is based on the orientation or position relationship shown in the accompanying drawings of the specification, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention.

[0031] The terms "first", "second", "third", and "fourth" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first", "second", "third", and "fourth" may explicitly or implicitly include at least one of the features.

[0032] The term "some specific embodiments" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same implementation or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0033] like Figure 1 As shown, an embodiment of the present invention provides a micro temperature control device, which includes a cover body 1, a connecting structure 2 and a base 3 connected in sequence from top to bottom. The cover body 1 and the base 3 both include a refrigeration structure, which is suitable for providing a low-temperature environment for printing and forming biological materials. The two ends of the connecting structure 2 are detachably connected to the cover body 1 and the base 3 respectively. The connecting structure 2 includes one or more connecting modules, and the multiple connecting modules are vertically stacked, and the number of the connecting modules is adapted to the printing height of the biological material.

[0034] It should be noted that the printing height in this embodiment specifically refers to the height of the product after the biomaterial is finally formed. It should also be noted that the two ends of the connection structure 2 in this embodiment specifically refer to the upper and lower ends of the connection structure 2 along the Y direction.

[0035] The micro temperature control device in this embodiment, on the one hand, realizes the cooling of the air near the cover body 1 and the base 3 at the same time by setting a refrigeration structure in the cover body 1 and the base 3, which can greatly reduce the temperature gradient inside the entire device, make the temperature inside the entire device tend to be uniform, and is more conducive to inkjet biological 3D printing and improve the printing effect; on the other hand, the number of connecting modules is adapted to the printing height of the biological material, that is, the number of connecting modules can be adjusted according to the height of the biological product, so that the vertical height of the multiple connecting modules is suitable for the printing and forming of the biological product, and the application range is wider.

[0036] like Figure 2 , Figure 4 and Figure 5 As shown, in some preferred embodiments, the refrigeration structure includes a first refrigeration structure arranged on the cover body 1, the first refrigeration structure includes a vertically arranged first cooling element 12, a first semiconductor refrigeration element 17 and a cover plate 18, the first cooling element 12 and the cover plate 18 are detachably connected, and the first cooling element 12 is arranged above the entire first refrigeration structure, and the first semiconductor refrigeration element 17 is embedded between the first cooling element 12 and the cover plate 18.

[0037] In this embodiment, the first semiconductor refrigeration element 17 can cool on one side and generate a large amount of heat on the other side. Therefore, the first semiconductor refrigeration element 17 is connected to the first cooling element 12, and the cooling element is used to dissipate the heat generated by the first semiconductor refrigeration element 17 to prevent the first semiconductor refrigeration element 17 from being burned. The first semiconductor refrigeration element 17 does not require any refrigerant, can work continuously, has no vibration or noise during operation, has a long service life, and is easy to install.

[0038] In some specific embodiments, the first cooling member 12 is a water head connected to external circulating water, which has a simple structure and is easy to process.

[0039] In this embodiment, there is no restriction on the manner in which the first cooling member 12 and the cover plate 18 are detachably connected. In some preferred embodiments, the first cooling member 12 is bolted to the cover plate 18 via the first fixing member 14, and the first fixing member 14 is disposed above the first cooling member 12, so that the connection is convenient and firm.

[0040] It should be noted that in this embodiment, there is no restriction on the shape of the first fixing member 14, as long as the first cooling member 12 and the cover plate 18 can be fixed. In some specific embodiments, the cross-section of the first fixing member 14 is rectangular, and screw holes cooperating with the first bolt 13 are provided on both sides. The structure is simple, and the connection is convenient and firm.

[0041] like Figure 2 and Figure 3 As shown, in some preferred embodiments, the cover body 1 further includes a heat preservation cover arranged around the first refrigeration structure. Thus, the heat preservation cover is sleeved on the outer surface of the first refrigeration structure to prevent the external air from affecting the temperature inside the cover body 1 and affecting the printing effect.

[0042] In some specific embodiments, the first refrigeration structure includes a cover plate 18, and two first semiconductor refrigeration components 17 and two first cooling components 12 are respectively arranged at both ends of the cover plate 18 in the horizontal direction. In this embodiment, there is no restriction on the shape of the insulation cover. In some preferred embodiments, the insulation cover includes an insulation top plate 16 arranged between the two first semiconductor refrigeration components 17 and the two first cooling components 12, an insulation side plate 15 arranged around the side wall of the cover plate 18, and an insulation cover 11 arranged around and above the first cooling components 12, which can fully insulate the interior of the cover body 1 to prevent external air from affecting the temperature inside the cover body 1 and affecting the printing effect.

[0043] In some preferred embodiments, a printing window suitable for the entry of biological materials is provided in the middle of the heat-insulating cover 11 and the cover plate 18 .

[0044] like Figure 5As shown, in some preferred embodiments, the bottom of the cover plate 18 is extended toward the connection structure 2 to be provided with a first protruding structure 19 that cooperates with the connection structure 2 .

[0045] like Figure 6-8 As shown, in some preferred embodiments, the connection module includes two oppositely arranged first connecting members 22 and a second connecting member 23 arranged between the two first connecting members 22, the second connecting member 23 is used to place the observation lens, and the first connecting member 22 and the second connecting member 23 are detachably connected.

[0046] In this embodiment, the first connecting member 22 and the second connecting member 23 are made of aluminum, which has a wide range of sources and is easy to process.

[0047] The observation lens in this embodiment can be a transparent glass lens, so that the operator can observe the state of the internal biological material to be printed in real time during the printing process. Similarly, a high-speed camera can be used to capture the gelation process of the biological ink dripping, which is used to improve the printing parameters of the printer; the observation lens in this embodiment can also be a germanium lens suitable for observation by an infrared observation device, which can allow infrared rays to pass through, thereby facilitating the use of an external infrared observation device to observe the internal temperature distribution.

[0048] In some preferred embodiments, a first positioning groove 24 is provided at the top end of the first connecting member 22 and the second connecting member 23, and a second protruding structure is provided at the bottom end of the first connecting member 22 and the second connecting member 23. The adjacent first connecting members 22 and the second connecting members 23 are detachably connected, and the first positioning groove 24 cooperates with the first protruding structure 19 or the first positioning groove 24 cooperates with the second protruding structure of the adjacent first connecting member 22 and the second connecting member 23 in the vertical direction, and the second protruding structure of the first connecting member 22 and the second connecting member 23 close to the base 3 in the vertical direction is also cooperated with the base 3.

[0049] In this embodiment, there is no restriction on the shapes of the first protrusion structure 19, the second protrusion structure and the first positioning groove 24. As long as the first protrusion structure 19 and the second protrusion structure can be respectively adapted to the shapes of the first positioning groove 24, the connection between the cover plate 18 and the first connecting member 22 and the second connecting member 23 can be achieved through the cooperation between the first protrusion structure 19 and the first positioning groove 24, and the connection between the first connecting member 22 and the second connecting member 23 adjacent in the vertical direction can be achieved through the cooperation between the second protrusion structure and the first positioning groove 24. In some specific embodiments, the first protrusion structure 19 is a circle of protrusions arranged at the bottom of the cover plate 18, which has a simple structure and is easy to connect.

[0050] like Figure 8As shown, in this embodiment, the third protrusion structure and the fourth protrusion structure are respectively arranged at both ends of the second connecting member 23 in the horizontal direction close to the two ends of the first connecting member 22. Correspondingly, the end portions of the first connecting member 22 respectively connected to the two ends of the second connecting member 23 in the horizontal direction are respectively arranged with a third positioning groove cooperating with the third protrusion structure and a fourth positioning groove cooperating with the fourth protrusion structure. The structure is simple and the connection is convenient.

[0051] like Figure 7 As shown, in some preferred embodiments, the connection module includes a heat preservation sleeve 21, and the first connection member 22 and the second connection member 23 are connected to each other to form a connection body, and the heat preservation sleeve 21 is arranged around the peripheral wall of the connection body. It should be noted that the shape of the heat preservation sleeve 21 matches the shape of the outer wall of the connection body, so that the heat preservation sleeve 21 can keep the connection module warm, prevent the external air from affecting the temperature inside the connection module and affecting the printing effect.

[0052] A plurality of the connecting modules are stacked vertically, including a plurality of connecting bodies arranged in sequence in the vertical direction, and the positions of the adjacent connecting bodies in the vertical direction correspond to each other. In some specific implementations, the insulating sleeve 21 of the connecting body corresponds one-to-one with the position of the insulating sleeve 21 of the connecting body adjacent to the vertical direction, the first connecting member 22 of the connecting body corresponds one-to-one with the position of the first connecting member 22 of the connecting body adjacent to the vertical direction, and the second connecting member 23 of the connecting body corresponds one-to-one with the position of the second connecting member 23 of the connecting body adjacent to the vertical direction.

[0053] like Figure 9-11 As shown, in some preferred embodiments, the refrigeration structure includes a second refrigeration structure arranged on the base 3, the second refrigeration structure includes a second cooling element 36, a second semiconductor refrigeration element 39 and a bottom plate 35, the second cooling element 36 and the bottom plate 35 are detachably connected, and the second semiconductor refrigeration element 39 is embedded between the second cooling element 36 and the bottom plate 35.

[0054] In this embodiment, the second semiconductor refrigeration element 39 can cool on one side and generate a large amount of heat on the other side. Therefore, the second semiconductor refrigeration element 39 is connected to the second cooling element 36, and the second cooling element 36 is used to dissipate the heat generated by the second semiconductor refrigeration element 39 to prevent the second semiconductor refrigeration element 39 from being burned. The second semiconductor refrigeration element 39 does not require any refrigerant, can work continuously, has no vibration or noise during operation, has a long service life, and is easy to install.

[0055] In some specific embodiments, the second cooling member 36 is a water head connected to external circulating water, which has a simple structure and is easy to process.

[0056] like Fig.11As shown, in this embodiment, there is no restriction on the manner in which the second cooling member 36 and the base plate 35 are detachably connected. In some preferred embodiments, the second cooling member 36 is bolted to the base plate 35 via a second fixing member 38, and the second fixing member 38 is disposed above the second cooling member 36, so that the connection is convenient and firm.

[0057] It should be noted that in this embodiment, there is no restriction on the shape of the second fixing member 38, as long as the second cooling member 36 can be fixed to the base plate 35. In some specific embodiments, the cross-section of the second fixing member 38 is rectangular, and screw holes that cooperate with the second bolts 37 are provided on both sides. The structure is simple, and the connection is convenient and firm.

[0058] In some preferred embodiments, the bottom plate 35 is provided with a second positioning groove 34 cooperating with the second protruding structure near the top of the connecting structure 2. The second protruding structure cooperates with the second positioning groove 34 to realize the connection between the connecting module and the bottom plate 35, and the connection is convenient and firm.

[0059] In some preferred embodiments, heat-conducting materials are respectively arranged between the first cooling element 12, the first semiconductor cooling element 17 and the cover plate 18, and heat-conducting materials are respectively arranged between the second cooling element 36, the second semiconductor cooling element 39 and the base plate 35. In this embodiment, there is no restriction on the specific type of heat-conducting material. In some specific embodiments, the heat-conducting material is thermal grease, which has a wide range of sources.

[0060] In some specific embodiments, the connection structure 2 includes two connection modules, namely a first connection module and a second connection module, and the first connection module is arranged above the second connection module. Then, the entire micro temperature control device includes a cover body 1, a first connection module, a second connection module and a base 3 connected in sequence from top to bottom, wherein the top end of the base 3 is provided with a second positioning groove 34 that cooperates with the second connection module, the top end of the second connection module is provided with a first positioning groove 24 that cooperates with the second protruding structure at the bottom end of the first connection module, the bottom end of the second connection module is provided with a second protruding structure that cooperates with the second positioning groove 34, and the top end of the first connection module is provided with a first positioning groove 24 that cooperates with the first protruding structure 19 of the cover body 1. The structure is simple and the connection is convenient.

[0061] In some preferred embodiments, the base 3 also includes an insulation structure, which includes a first insulation component 31 arranged on the side wall of the second refrigeration structure and a second insulation component 32 arranged on the top wall of the second refrigeration structure, and the first insulation component 31 and the second insulation component 32 are both detachably connected to the bottom plate 35.

[0062] In some preferred embodiments, the first heat-insulating member 31 is connected to the bottom plate 35 by a third bolt 33 , and the connection is convenient and firm.

[0063] In this embodiment, there is no limitation on the shapes of the first thermal insulation component 31 and the second thermal insulation component 32. In some preferred embodiments, the cross-sectional shape of the first thermal insulation component 31 and the second thermal insulation component 32 is rectangular, with a simple structure, and can insulate the base 3 to prevent external air from affecting the temperature inside the base 3 and affecting the printing effect.

[0064] In this embodiment, the micro temperature control device realizes the cooling of the air near the cover 1 and the base 3 by setting a refrigeration structure in the cover 1 and the base 3 at the same time, which can greatly reduce the temperature gradient inside the entire device and make the low temperature inside the entire device uniform. At this time, when the high-temperature liquid biological material drips into the entire device from the printing window of the cover 1, the biological material exchanges heat with the air through the low temperature inside the entire device, thereby solidifying into solid biological material. The biological material accumulates layer by layer to form a biological product with a special shape. If a higher biological product needs to be printed, multiple connection modules can be stacked to increase the height of the entire device, thereby printing a higher biological product.

[0065] Therefore, the micro-temperature control device in the embodiment of the present invention, on the one hand, realizes the cooling of the air near the cover body 1 and the base 3 by setting a refrigeration structure in the cover body 1 and the base 3, which can greatly reduce the temperature gradient inside the entire device, make the temperature inside the entire device tend to be uniform, and is more conducive to inkjet biological 3D printing and improve the printing effect; on the other hand, the number of connecting modules is adapted to the printing height of the biological material, that is, the number of connecting modules can be adjusted according to the height of the biological product, and the application range is wider.

[0066] Another embodiment of the present invention also provides an inkjet biological 3D printer, including an infrared observation device and a micro temperature control device.

[0067] The advantages of the inkjet biological 3D printer and the micro temperature control device of the present invention are the same as those of the prior art, which will not be repeated here.

[0068] Although the present invention is disclosed as above, the protection scope of the present invention is not limited thereto. Those skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications will fall within the protection scope of the present invention.

Claims

1. A micro temperature control device, characterized in that: The invention comprises a cover body, a connection structure and a base which are sequentially connected from top to bottom, wherein the cover body and the base both comprise a refrigeration structure, wherein the refrigeration structure is suitable for providing a low-temperature environment for printing and forming biological materials, wherein two ends of the connection structure are detachably connected to the cover body and the base respectively, wherein the connection structure comprises a plurality of connection modules, wherein the plurality of connection modules are vertically stacked, and the number of the connection modules is adapted to the printing height of the biological materials, and the high-temperature liquid biological materials are used to solidify into solid biological products on the base through the cover body and the connection structure in sequence, wherein the connection module comprises two first connection members which are arranged opposite to each other and a second connection member which is arranged between the two first connection members, wherein the second connection member is used for placing an observation lens, and the first connection member is connected to the The second connecting member is detachably connected, and a first positioning groove is provided at the top end of the first connecting member and the second connecting member, and a second protrusion structure is provided at the bottom end of the first connecting member and the second connecting member. The adjacent first connecting member and the second connecting member are detachably connected, and the first positioning groove cooperates with the first protrusion structure or the first positioning groove cooperates with the second protrusion structure of the first connecting member and the second connecting member adjacent to each other in the vertical direction, and the second protrusion structure of the first connecting member and the second connecting member close to the base in the vertical direction is also cooperated and connected with the base, and the connecting module includes an insulation cover, and the first connecting member and the second connecting member are connected to each other to form a connecting body, and the insulation cover is arranged around the peripheral wall of the connecting body.

2. The micro temperature control device according to claim 1, characterized in that: The refrigeration structure includes a first refrigeration structure arranged on the cover body, the first refrigeration structure includes a first cooling member, a first semiconductor cooling member and a cover plate, the first cooling member is detachably connected to the cover plate, and the first semiconductor cooling member is embedded between the first cooling member and the cover plate.

3. The micro temperature control device according to claim 2, characterized in that: The bottom of the cover plate is extended in a direction close to the connection structure to form a first protrusion structure matched with the connection structure.

4. The micro temperature control device according to claim 1, characterized in that: The vertical stacking of the plurality of connection modules comprises a plurality of connection bodies being arranged in sequence in the vertical direction, and positions of adjacent connection bodies in the vertical direction correspond to each other.

5. The micro temperature control device according to claim 1, characterized in that: The refrigeration structure also includes a second refrigeration structure arranged on the base, the second refrigeration structure includes a second cooling element, a second semiconductor refrigeration element and a bottom plate, the second cooling element is detachably connected to the bottom plate, the second semiconductor refrigeration element is embedded between the second cooling element and the bottom plate, and the bottom plate is provided with a second positioning groove that cooperates with the second protrusion structure near the top of the connecting structure.

6. The micro temperature control device according to claim 5, characterized in that: The base also includes a heat preservation structure, which includes a first heat preservation component arranged on the side wall of the second refrigeration structure and a second heat preservation component arranged on the top wall of the second refrigeration structure, and the first heat preservation component and the second heat preservation component are both detachably connected to the bottom plate.

7. An inkjet biological 3D printer, characterized in that: It comprises an infrared observation device and a micro temperature control device as described in any one of claims 1 to 6.

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