Specific protein analyzer, measurement method and computer-readable storage medium

Abstract

The present application provides a specific protein analyzer, which includes a sample supply device, a first reagent supply device, a second reagent supply device, a reaction cell, a mixing assembly, a buffer assembly, a detection device, and a control device. The sample supply device, the first reagent supply device, and the second reagent supply device are respectively used to supply a blood sample to be tested, a first reagent, and a second reagent to the reaction cell. The mixing assembly mixes the mixed sample solution, and then the control device controls the buffer assembly to suck out the mixed sample solution in the middle of the reaction cell for buffering, and accepts the specific protein content detection by the detection device. The mixed sample solution in the middle of the reaction cell is relatively uniform and contains fewer bubbles, so more accurate detection results can be obtained. The present application also provides a specific protein determination method and a computer-readable storage medium for implementing this method.

Description

Technical Field

[0001] This application relates to the technical field of medical devices, and particularly to a specific protein analyzer, a specific protein determination method, and a computer-readable storage medium. Background Art

[0002] With the popularization of clinical applications, more and more parameters need to be detected in the field of blood tests. From the initial three-classification and five-classification parameters of blood routine to the later specific protein parameters, such as CRP (C-Reactive Protein) parameters. The detection of specific protein parameters generally uses transmission and / or turbidimetry for detection.

[0003] To detect the content of a specific protein (antigen), specific latex particles (antibodies) need to be added to the blood sample. The latex particles are nanoscale spherical particles, which can react and bind with the surrounding specific proteins under certain conditions to form micelles with a larger volume. When the latex particles continuously bind to the specific proteins, the formed micelles gradually increase. The scattered signal formed after irradiation with light of a specific wavelength gradually increases, and the transmitted signal gradually weakens. By monitoring the rate of change of the transmitted and / or scattered signals and through certain calculations, the content of the specific protein in the blood sample can be obtained. In the case of using whole blood samples for detection, a hemolytic agent needs to be added to the blood sample before adding the specific latex particles to dissolve the blood cells in the blood sample.

[0004] Due to the relatively large fluid properties such as viscosity and density of the latex reagent, it is necessary to mix the blood sample and the latex reagent before performing the specific protein detection to avoid affecting the detection results. A specific protein analyzer usually has a reaction cell for mixing. During the liquid addition process of the reaction cell itself, a part of the liquid is affected by gravity and leaks into the drain pipeline, and this part of the leaked liquid is difficult to be affected during the subsequent mixing process, resulting in local non-uniformity of the mixed sample liquid; at the same time, bubbles are easily generated in the mixed sample liquid during the mixing process. Although these bubbles will eventually float to the liquid surface, if there are occasionally large bubbles, they will also interfere with the detection results. Summary of the Invention

[0005] This application provides a specific protein analyzer with higher detection accuracy, a specific protein determination method with higher detection accuracy, and a computer-readable storage medium for implementing this method. Specifically, the following solutions are included:

[0006] In a first aspect, this application provides a specific protein analyzer, including:

[0007] A sample supply device for providing a blood sample to be tested;

[0008] A first reagent supply device for providing a first reagent that reacts with the blood sample to be tested;

[0009] A second reagent supply device for providing a second reagent that reacts with the blood sample to be tested;

[0010] A reaction cell that receives the blood sample to be tested supplied by the sample supply device, the first reagent supplied by the first reagent supply device, and the second reagent supplied by the second reagent supply device, so that the blood sample to be tested reacts with the first reagent and the second reagent to form a mixed sample solution;

[0011] A mixing assembly for mixing the mixed sample solution in the reaction cell;

[0012] A buffer assembly including a buffer channel and a buffer power device, the buffer channel including a first end and a second end and being filled with liquid, the first end of the buffer channel communicating with the inner cavity of the reaction cell, and the second end of the buffer channel being connected to the buffer power device;

[0013] A detection device including a detection area made of a light-transmitting material and a light source correspondingly arranged with the detection area, the light source being configured to irradiate the mixed sample solution in the detection area so as to detect the content of a specific protein in the mixed sample solution;

[0014] A control device configured to:

[0015] Control the buffer power device to drive the liquid in the buffer channel to flow away from the reaction cell, suck out the mixed sample solution located in the middle of the reaction cell into the buffer channel for buffering, for detecting the content of a specific protein.

[0016] In a second aspect, the present application provides a method for determining a specific protein, which is applied to the above-mentioned specific protein analyzer, and includes the following steps:

[0017] Add a blood sample to be tested and reagents required for testing to the reaction cell to form a mixed sample solution;

[0018] Mix the mixed sample solution;

[0019] Extract the mixed sample solution located in the middle of the reaction cell and temporarily store it in the buffer channel;

[0020] Detect the mixed sample solution buffered in the buffer channel.

[0021] In a third aspect, the present application further provides a computer-readable storage medium storing executable instructions, which are configured to cause a processor to implement the above-mentioned method for determining a specific protein when executing the executable instructions.

[0022] The specific protein analyzer provided in the first aspect of the present application respectively provides a blood sample to be tested into the reaction cell through the sample supply device, a first reagent through the first reagent supply device, and a second reagent through the second reagent supply device, so as to configure and form a mixed sample solution in the reaction cell. Then, after being mixed by the mixing component, the control device controls the buffer component to suck out the mixed sample solution located in the middle of the reaction cell for buffering. This part of the mixed sample solution has advantages such as relatively uniform and less bubble content. Therefore, when the detection device finally detects the buffered mixed sample solution, higher detection accuracy can be obtained.

[0023] It can be understood that the specific protein determination method provided in the second aspect of the present application, and the computer-readable storage medium provided in the third aspect, also detect the same part of the mixed sample solution due to similar solutions, and thus obtain higher detection accuracy. Description of the Drawings

[0024] Figure 1 is a schematic structural framework diagram of a specific protein analyzer provided by an embodiment of the present application;

[0025] Figure 2 is a schematic structural diagram of the reaction cell of a specific protein analyzer provided by an embodiment of the present application;

[0026] Figure 3 is a schematic liquid path diagram of a specific protein analyzer provided by an embodiment of the present application;

[0027] Figure 4 is a schematic liquid path diagram of a specific protein analyzer provided by another embodiment of the present application;

[0028] Figure 5 is a schematic liquid path diagram of a specific protein analyzer provided by still another embodiment of the present application;

[0029] Figure 6 is a flowchart of a specific protein determination method provided by an embodiment of the present application;

[0030] Figure 7 is a sub-step flowchart of step S20 in a specific protein determination method provided by an embodiment of the present application;

[0031] Figure 8 is a sub-step flowchart of step S21 in a specific protein determination method provided by an embodiment of the present application;

[0032] Figure 9 is a sub-step flowchart of step S10 in a specific protein determination method provided by an embodiment of the present application;

[0033] Figure 10It is a flowchart of sub-steps of step S12 in a specific protein determination method provided by an embodiment of the present application;

[0034] Figure 11 It is a flowchart of a specific protein determination method provided by another embodiment of the present application;

[0035] Figure 12 It is a schematic diagram of a computer-readable storage medium provided by an embodiment of the present application. Detailed implementation manners

[0036] Now, reference will be made in detail to the embodiments, examples of which are shown in the accompanying drawings. Many specific details are set forth in the following detailed description in order to provide a thorough understanding of the various described embodiments. However, it should be understood by those of ordinary skill in the art that the various described embodiments may be implemented without these specific details. In other embodiments, well-known methods, procedures, components, circuits, and networks are not described in detail so as not to unnecessarily obscure the embodiments.

[0037] It will also be understood that although in some cases the terms "first", "second", etc. are used herein to describe various elements or other objects, these elements or objects should not be limited by these terms. These terms are only used to distinguish one element / object from another element / object.

[0038] In the description of the various embodiments herein, the terms used are for the purpose of describing specific embodiments only and are not intended to be limiting. As used in the description of the various embodiments and the appended claims, the singular forms "a" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will also be understood that the term "and / or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items. It will also be understood that the term "comprises" as used in this specification means that there are the stated features, steps, operations, elements, and / or components, but does not preclude the presence or addition of one or more other features, steps, operations, elements, and / or components.

[0039] As used herein, depending on the context, the term "if" can be interpreted to mean "when", "in response to determining", or "in response to detecting", etc. Similarly, depending on the context, the phrase "if it is determined that..." or "if it is detected that [the stated condition or event]" can be interpreted to mean "when it is determined that...", "in response to determining...", "when it is detected that [the stated condition or event]" or "in response to detecting [the stated condition or event]".

[0040] Please refer to Figure 1A specific protein analyzer 100 provided by an embodiment of the present invention as shown includes a sample supply device 80, a first reagent supply device 10, a second reagent supply device 20, a reaction cell 30, a mixing assembly 50, a buffer assembly 60, a detection device 70, and a control device 40. The reaction cell 30 is used to provide a receiving space so that the blood sample to be tested provided by the sample supply device 80, the first reagent provided by the first reagent supply device 10, and the second reagent provided by the second reagent supply device 20 can converge in the receiving space in a preset ratio and form a mixed sample solution for specific protein determination after mixing. It can be understood that both the first reagent and the second reagent are used to react with the blood sample to be tested, so that the blood cells in the blood sample to be tested can be fully dissolved, and the specific proteins in the blood cells can form particles of a measurable size.

[0041] In one embodiment, the first reagent can be a hemolytic agent, which is used to promote the dissolution of blood cells. In one embodiment, the second reagent can be a latex reagent, which is a suspension containing latex particles. The latex particles are nanoscale spherical particles and can react and bind with the surrounding specific proteins to form micelle particles of a measurable size.

[0042] Because the fluid properties such as viscosity and density of the latex reagent are relatively large, it is difficult to mix the blood sample to be tested with the latex reagent. If the mixing is uneven, it may affect the detection accuracy of the specific protein analyzer 100 of the present application. The mixing assembly 50 is used to mix the liquid in the reaction cell 30 so that after two or more liquids are received in the reaction cell 30, the liquid in the reaction cell 30 can be mixed until the mixed sample solution is finally formed, and the mixing work of the mixed sample solution is completed. The mixing assembly 50 can be realized in various ways such as a stirring paddle, a robotic arm, or a pipeline with the ability to aspirate and mix the sample solution.

[0043] The detection device 70 is used to detect the mixed sample solution that has been mixed. It includes a detection area 71 and a light source 72. The detection area 71 is made of a light-transmitting material and is used to hold the mixed sample solution. The light source 72 is arranged corresponding to the detection area 71. The light emitted by the light source 72 irradiates the light-transmitting detection area 71, and the light enters the mixed sample solution held in the detection area 71. Part of the light is refracted by the micelle particles formed by the binding of specific proteins and latex particles and exits from one side of the detection area 71, and the other part of the light directly passes through the detection area 71 along the incident light direction and exits. Since the intensity of the light emitted by the light source 72 is controllable and known, by detecting the intensity of the refracted light or transmitted light emitted from the detection area 71, the content of the micelle particles in the detection area can be deduced, and then the content of the specific protein in the mixed sample solution can be calculated.

[0044] The buffer component 60 is used to extract the mixed sample liquid for detection from the reaction pool 30. The buffer component 60 includes a buffer channel 61 and a buffer power device 62. The buffer channel 61 includes opposite first end 611 and second end 612 along its extending path, and the buffer channel 61 is filled with liquid, that is, the space between the first end 611 and the second end 612 is filled with liquid. Wherein the first end 611 of the buffer channel 61 is communicated with the inner cavity 301 of the reaction pool 30 (please refer to Figure 2 ), the second end 612 of the buffer channel 61 is connected to the buffer power device 62, and the buffer power device 62 is used to provide positive pressure or negative pressure for the liquid in the buffer channel 61, so that the liquid in the buffer channel 61 is pushed into the reaction pool 30, or the liquid in the reaction pool 30 is sucked into the buffer channel 61.

[0045] The control device 40 is electrically connected to each of the above components respectively, and is used to control the coordinated work of each component and realize the specific protein detection work on the blood sample to be tested. Specifically, the working principle process of the specific protein analyzer 100 provided in this embodiment is as follows:

[0046] The blood sample to be tested, the first reagent and the second reagent are respectively added into the reaction pool 30 through the sample supply device 80, the first reagent supply device 10 and the second reagent supply device 20 to form a mixed sample liquid. However, at this time, the blood sample to be tested, the first reagent and the second reagent are not fully mixed and are in an uneven state. It is necessary to fully mix them before the specific protein detection can be carried out, and thus a mixing operation is required.

[0047] After the mixing component 50 fully mixes the mixed sample liquid, the control device 40 controls the buffer power device 62 to work to provide negative pressure, driving the liquid in the buffer channel 61 to flow away from the reaction pool 30, sucking out a part of the mixed sample liquid in the reaction pool 30 into the buffer channel 61 for buffering, and then the detection device 70 detects the specific protein content of the buffered part of the mixed sample liquid to obtain detection data. Specifically, the buffer channel 61 also needs to be communicated with the detection area 71 of the detection device 70, or at least part of the buffer channel 61 is made of a light-transmitting material, and this part of the buffer channel 61 made of the light-transmitting material serves as the detection area 71. After this part of the mixed sample liquid is sent into the detection area 71, the detection device 70 can obtain detection data through the detection of this part of the mixed sample liquid.

[0048] For an embodiment, please refer to Figure 2The structure of the reaction cell 30 shown. The reaction cell 30 includes an inner cavity 301, a top opening 302, a side wall 303, and a bottom surface 304. A side inlet 31 is further provided on the side wall 303 of the reaction cell 30, and this side inlet 31 can serve as the first end 611 of the buffer channel 61. A waste liquid discharge port 32 is further provided on the bottom surface 304 of the reaction cell 30, and the waste liquid discharge port 32 is communicated with a liquid discharge pipeline (not shown in the figure). It can be understood that after the mixed sample liquid is tested, it can flow into the liquid discharge pipeline through the waste liquid discharge port 32 and be sent out of the specific protein analyzer 100.

[0049] The side inlet 31 is provided at a position near the bottom surface 304 in the middle of the side wall 303 of the reaction cell 30. This enables the buffer assembly 60 to suck out a part of the mixed sample liquid in the middle area of the reaction cell 30 into the buffer channel 61 when sucking out the mixed sample liquid in the reaction cell 30 through the first end 611 of the buffer channel 61. As mentioned above, during the mixing process of the mixed sample liquid by the mixing assembly 50, since the liquid near the waste liquid discharge port 32 is difficult to be affected, the lower liquid of the mixed sample liquid is prone to uneven mixing; and during the mixing process of the mixed sample liquid by the mixing assembly 50, bubbles may be generated in the mixed sample liquid. These generated bubbles will eventually float to the top liquid surface position of the mixed sample liquid because of their light mass. When the bubble volume is too large, it is also easy to interfere with the detection of the mixed sample liquid. That is, there are defects in the uneven mixing of the bottom area of the mixed sample liquid in the reaction cell 30, and there are bubble defects in the top area of the mixed sample liquid in the reaction cell 30, while the mixed sample liquid in the middle area of the reaction cell 30 has a relatively good mixing effect and fewer bubbles, and can more accurately reflect the true content of the specific protein in the blood sample to be tested.

[0050] Therefore, by using the side inlet 31 provided in the middle of the reaction cell 30 as the first end 611 of the buffer assembly 60, the specific protein analyzer 100 of the present application can enable the control device 40 to suck out a part of the mixed sample liquid with higher quality in the middle area of the mixed sample liquid contained in the reaction cell 30 into the buffer channel 61 when controlling the buffer power device 62 to form a negative pressure. Subsequently, more accurate detection data can be obtained by detecting this part of the mixed sample liquid by the detection device 70.

[0051] Please refer to Figure 3Schematic diagram of a liquid path of an embodiment of the specific protein analyzer 100 of the present application. In this embodiment, the detection area 71 of the detection device 70 is configured as the reaction cell 30. At least a part of the side wall 303 of the reaction cell 30 is made of a light-transmitting material, so that a part of the inner cavity 301 can transmit light and is configured as the detection area 71. Since the reaction cell 30 is an essential structure, and the reaction cell 30 is constructed as the detection area 71, the structure of a separate colorimetric cell for detection can be omitted, thereby controlling the cost of the specific protein analyzer 100.

[0052] The side inlet 31 includes a first side inlet 311 located on the left side of the illustrated reaction cell 30 and a second side inlet 312 located on the right side of the illustrated reaction cell 30. The first side inlet 311 communicates with the first reagent supply device 10, and the first reagent supply device 10 includes a first reagent container 13, a first reagent syringe 12, and a first solenoid valve 11. The first solenoid valve 11 is used to control the on-off between the first reagent container 13 and the first side inlet 311. When the first solenoid valve 11 connects the first reagent container 13 and the first side inlet 311, the first reagent syringe 12 can suck out the first reagent in the first reagent container 13 and inject the first reagent into the reaction cell 30 through the first side inlet 311.

[0053] The second reagent supply device 20 and the sample supply device 80 are not shown in the figure, and both can be implemented by a sampling needle (not shown in the figure) configured at the top opening 302 of the reaction cell 30. The sampling needle sucks the blood sample to be tested from the container containing the blood sample to be tested and spits it into the inner cavity 301 through the opening 302; it also sucks the latex reagent from the container storing the latex reagent and spits it into the inner cavity 301 through the opening 302. Alternatively, in some embodiments, the sampling needle can also be directly connected to the container storing the latex reagent through an internal pipeline and directly spit the latex reagent into the inner cavity 301 through this pipeline.

[0054] The mixing assembly 50 can be implemented in the form of a stirring paddle or a robotic arm, and is not shown in Figure 3 the embodiment either. A drain valve 321 and a waste liquid bucket 322 can be provided below the waste liquid outlet 32. The reaction cell 30 discharges the mixed sample liquid that has completed the detection into the waste liquid bucket 322 through the drain valve 321. In order to ensure that the liquid in the reaction cell 30 is emptied, a waste liquid pump 323 can also be provided outside the waste liquid outlet 32 for pumping the liquid in the reaction cell 30.

[0055] The second-side inlet 312 is connected to the buffer component 60, that is, the second-side inlet 312 serves as the first end 611 of the buffer channel 61. The second end 612 of the buffer channel 61 is connected to the buffer power device 62, and a second solenoid valve 613 is also provided in the buffer channel 61. The second solenoid valve 613 is used to control the on-off between the buffer power device 62 and the second-side inlet 312. When the second solenoid valve 613 connects the buffer power device 62 and the second-side inlet 312, the buffer power device 62 can provide a positive pressure or a negative pressure to the buffer channel 61, and suck the liquid in the reaction tank 30 into the buffer channel 61, or spit the liquid in the buffer channel 61 back into the reaction tank 30.

[0056] From Figure 3 It can be seen that the sample analyzer 100 further includes a cleaning liquid supply device 91. The cleaning liquid supply device 91 is connected to the buffer component 60, specifically to the buffer channel 61 of the buffer component 60. The cleaning liquid supply device 91 is used to supply cleaning liquid to the reaction tank 30 and the buffer channel 61, so as to realize the cleaning operation of the reaction tank 30 and the buffer channel 61 before and after each detection, and avoid the contamination of the current mixed sample liquid due to the residue of waste liquid.

[0057] As mentioned above, when the specific protein analyzer 100 of the present application is put into use, it is first necessary to fill the buffer component 60 with liquid to empty the gas in the buffer channel 61 and ensure the accuracy of the detection results. Therefore, after the cleaning liquid supply device 91 is connected to the buffer component 60, the buffer channel 61 can be filled with cleaning liquid. The cleaning liquid in the specific protein analyzer 100 is usually realized by using pure water or pure water added with a small amount of cleaning agent, and its chemical composition is relatively simple. Therefore, when the buffer power device 62 sucks and spits the mixed sample liquid in the reaction tank 30, although a small part of the mixed sample liquid first sucked into the buffer channel 61 will come into contact with the cleaning liquid, since the cleaning liquid has little influence on the mixed sample liquid, it will not have too much influence on the mixed sample liquid.

[0058] Of course, in order to avoid the influence of the cleaning liquid on the mixed sample liquid, the diameter of the first end 611 of the buffer channel 61 can be set smaller so that the cleaning liquid in the buffer channel 61 cannot overflow into the reaction tank 30. In other embodiments, it is also possible to avoid the liquid in the buffer channel 61 flowing into the reaction tank 30 due to gravity by increasing the height of the setting position of the first end 611 relative to the second end 612.

[0059] It should be noted that Figure 3 For the convenience of illustration, the first-side inlet 311 and the second-side inlet 312 are arranged on both sides of the reaction tank 30. In the actual structure of the reaction tank 30, its first-side inlet 311 and second-side inlet 312 can be as Figure 2As shown, the two are arranged side by side. Alternatively, in other embodiments, the relative positions of the first side inlet 311 and the second side inlet 312 are not limited, which will not affect the normal operation of the specific protein analyzer 100 of the present application.

[0060] Please refer to Figure 4 In the embodiment of, the mixing assembly 50 is arranged in a suction and discharge mixing manner in this embodiment. The mixing assembly 50 includes a mixing channel 51 and a mixing power device 52. The mixing channel 51 also includes opposite first end 511 and second end 512 along its extending path, and the mixing channel 51 is also filled with liquid. The first end 511 of the mixing channel 51 is also communicated with the inner cavity 301 of the reaction cell 30, and the second end 512 of the mixing channel 51 is also connected to the mixing power device 52. The mixing power device 52 is also used to provide positive pressure or negative pressure for the liquid in the mixing channel 51, so that the liquid in the mixing channel 51 is pushed into the reaction cell 30, or the liquid in the reaction cell 30 is sucked out into the mixing channel 51.

[0061] When performing the mixing operation, the control device 40 is further configured to control the mixing power device 52 to first drive the liquid in the mixing channel 51 to move away from the reaction cell 30, so as to suck a part of the liquid in the reaction cell 30 into the mixing channel 51. Then the mixing power device 52 drives the liquid in the mixing channel 51 to move towards the reaction cell 30, so as to push a part of the liquid in the mixing channel 51 into the reaction cell 30, forming a swirl in the reaction cell 30. Repeating this cycle, the blood sample to be tested, the first reagent, and the second reagent in the reaction cell 30 are mixed.

[0062] In Figure 4 In the schematic diagram of, the second side inlet 312 is simultaneously configured as the first end 511 of the mixing channel 51 to realize the connection between the mixing channel 51 and the reaction cell 30. Further, the cleaning liquid supply device 91 is also communicated with the mixing channel 51, so that the mixing channel 51 is filled with cleaning liquid in the initial state and the gas in the mixing channel 51 is emptied. Thereby, the generation of bubbles is avoided during the mixing operation of the mixing assembly 50, and the accuracy of specific protein detection can be improved compared with other mixing methods.

[0063] It can be understood that the specific protein analyzer 100 provided by the present application can control the liquid volume sucked from the reaction cell 30 into the mixing channel 51 and the liquid volume pushed from the mixing channel 51 into the reaction cell 30 by controlling the mixing power device 52, so that the dilution ratio is controllable and will not affect the detection result. Further, the second side inlet 312 is arranged on the side wall of the reaction cell 30 close to the bottom surface 304, and the first end 511 of the mixing channel 51 is below the liquid level of the mixed sample liquid in the reaction cell 30, so that the mixing power device 52 can suck liquid from the reaction cell 30 through the mixing channel 51.

[0064] From Figure 3 and Figure 4 In the embodiments of, for the mixing component 50 and the buffer component 60 of the present application, since both suck the mixed sample liquid from the reaction tank 30, and the second side inlet 312 can be respectively configured as the first end 511 of the mixing channel 51 and the first end 611 of the buffer channel 61, the functions of the mixing power device 52 and the buffer power device 62 are basically the same. Further, both the mixing channel 51 and the buffer channel 61 can be connected to the cleaning liquid supply device 91 to be filled with cleaning liquid in the mixing channel 51 and the buffer channel 61.

[0065] Therefore, in Figure 4 the embodiments of, the mixing component 50 and the buffer component 60 are also integrally provided. That is, in Figure 4 the embodiments, the mixing channel 51 and the buffer channel 61 are integrally provided, the mixing power device 52 is also integrally provided with the buffer power device 62, and the second side inlet 312 simultaneously serves as the first end 511 of the mixing channel 51 and the first end 611 of the buffer channel 61. Thus, the same liquid path channel can simultaneously serve as the mixing channel 51 and the buffer channel 61, and the same power device can simultaneously serve as the mixing power device 52 and the buffer power device 62. Such a setting can reduce the number of components of the specific protein analyzer 100 of the present application, compress the overall volume of the specific protein analyzer 100, and also achieve the beneficial effect of cost savings.

[0066] The specific protein analyzer 100 of the present application can also provide an embodiment. On the basis that the mixing component 50 and the buffer component 60 are integrally provided, the first reagent supply device 10 is further integrally provided with the mixing component 50 and the buffer component 60. That is, the mixing channel 51 and the buffer channel 61 can also be realized by borrowing the channel of the first reagent supply device 10, and the first reagent syringe 12 serves as both the mixing power device 52 and the buffer power device 62. The first solenoid valve 11 of the first reagent supply device 10 can also be used as the second solenoid valve 613.

[0067] At this time, since the first reagent container 13 is connected in the first reagent supply device 10, the liquid that can fill the pipelines configured as the mixing channel 51 and the buffer channel 61 is the first reagent. Or, by configuring a three-way valve in the first reagent supply device 10, the cleaning liquid supply device 91 can also be connected to the first reagent supply device 10 so that the liquid filling the mixing channel 51 and the buffer channel 61 is still the cleaning liquid.

[0068] It can be understood that after the first reagent supply device 10 is integrally provided with the mixing assembly 50 and the buffer assembly 60, only one side inlet 31 can be provided on the pool wall 303 of the reaction pool 30. The first reagent syringe 12 serves as the mixing power device 52 and the buffer power device 62, and realizes the suction and discharge actions of the liquid in the reaction pool 30 through the side inlet 31. Such a setting can further reduce the number of components of the specific protein analyzer 100 of the present application, and further compress the volume and control the cost.

[0069] In an embodiment, corresponding to the scenario where the reaction pool 30 is made of a light-transmitting material and configured as a detection area 71, the detection device 70 is correspondingly arranged with the detection area 71, that is, the light source 72 is correspondingly arranged with the reaction pool 30. The control device 40 is further configured to control the buffer power device 62 to drive the liquid in the buffer channel 61 to flow away from the reaction pool 30, suck the mixed sample liquid located in the middle of the reaction pool 30 into the buffer channel 61 for buffering, and then control the waste liquid discharge port 32 of the reaction pool 30 to open (that is, open the drain valve 321), and discharge the remaining mixed sample liquid in the reaction pool 30 to the outside of the reaction pool 30 (such as the waste liquid bucket 322). Then, the control device 40 further controls the buffer power device 62 to drive the liquid in the buffer channel 61 to flow towards the reaction pool 30, so as to push the mixed sample liquid in the buffer channel 61 into the reaction pool 30. Finally, the control device 40 controls the detection device 70 to detect the specific protein concentration of the mixed sample liquid in the reaction pool 30.

[0070] As mentioned above, after the buffer assembly 60 extracts the mixed sample liquid with higher quality in the middle area of the reaction pool 30, the mixed sample liquid remaining in the reaction pool 30 is the partially mixed sample liquid that is prone to uneven mixing in the lower part of the original reaction pool 30 and the mixed sample liquid with bubbles accumulated in the upper part of the original reaction pool 30. If this part of the remaining mixed sample liquid continues to stay in the reaction pool 30, when the buffer assembly 60 pushes the mixed sample liquid extracted from the buffer channel 61 into the reaction pool 30 again for measurement, it will affect the quality of the partially mixed sample liquid to be measured. Therefore, after the buffer assembly 60 extracts the mixed sample liquid in the middle area of the reaction pool 30, the mixed sample liquid in the lower part of the original reaction pool 30 and the mixed sample liquid in the upper part of the original reaction pool 30 remaining in the reaction pool 30 are first discharged from the reaction pool 30 through the waste liquid discharge port 32, and then the mixed sample liquid in the buffer channel 61 is pushed back into the reaction pool 30 for detection, which can ensure that the detection device 70 detects the mixed sample liquid with higher quality in the middle area of the original reaction pool 30, and its detection result can better reflect the true situation of the blood sample to be tested.

[0071] For an embodiment, please refer to Figure 5, a part of the buffer channel 61 is made of a light-transmitting material to serve as a detection area 71 of the detection device 70, and the light source 72 of the detection device 70 is arranged corresponding to the detection area 71. Thus, the control device 40 is further configured to control the buffer power device 62 to drive the liquid in the buffer channel 61 to flow away from the reaction tank 30, so as to suck the mixed sample liquid in the middle of the reaction tank 30 into the buffer channel 61 for buffering. Specifically, the part of the mixed sample liquid is correspondingly sucked into the detection area 71 for buffering. Then, the control detection device 70 is used to detect the concentration of specific proteins in the mixed sample liquid in the buffer channel 61.

[0072] In Figure 5 the embodiment, the occupied space is also reduced by bending the buffer channel 61, so that the overall volume of the specific protein analyzer 100 is reduced.

[0073] For an embodiment, please refer back to Figure 3 , the specific protein analyzer 1000 of the present application further includes a preheating device 92 for heating the liquid. The preheating device 92 is correspondingly arranged on the buffer channel 61 to heat the liquid in the buffer channel 61. In order to ensure the accuracy of specific protein detection, it is also necessary to make the temperature of the mixed sample liquid consistent during detection. Generally, the temperature of the mixed sample liquid needs to be maintained at about 37 °C. Usually, the first reagent, the second reagent, and the blood sample to be tested do not reach this temperature requirement before being added to the reaction tank 30. Especially when the second reagent is a latex reagent, it is usually in a low-temperature state. Therefore, by setting the preheating device 92 for heating the mixed sample liquid, it can be ensured that the specific protein detection work is carried out after the mixed sample liquid reaches the preset temperature, ensuring the accuracy and consistency of the detection.

[0074] In order to ensure the heating effect of the preheating device 92, usually, the preheating device 92 is set to continuously heat the mixed sample liquid in the buffer channel 61 for a certain period of time so that the mixed sample liquid can reach the preset temperature. In one embodiment, the preheating device 92 is set to heat the mixed sample liquid in the buffer channel 61 for a duration within 2-15 s. Further, in order to improve the heating effect of the preheating device 92, the preheating device 92 can be set as a heating rod, and the buffer channel 61 is spirally wound around the outside of the heating rod. Thereby, the contact area between the preheating device 92 and the buffer channel 61 can be increased, and the heating efficiency can be improved.

[0075] In one embodiment, the specific protein analyzer 100 may further include a blood routine detection module for classifying and / or counting cells in a blood sample to be tested. Specifically, the blood routine detection module may include at least one detection module among a WBC (white blood cell) classification measurement module, a WBC / HGB measurement module, and an RBC / PLT measurement module. The WBC classification measurement module is used to obtain the five-classification result of WBC in the blood sample to be tested. The WBC / HGB measurement module is used to complete the counting of WBC and the measurement of morphological parameters, and also has the function of measuring HGB (hemoglobin). The RBC / PLT measurement module is used to complete the counting of RBC (red blood cell) and PLT (blood platelet) and the measurement of morphological parameters.

[0076] Figure 6 The specific steps of the specific protein determination method provided in the second aspect of the present application are illustrated. This specific protein determination method can be applied to the above-mentioned specific protein analyzer 100, and specifically includes the following steps:

[0077] S10. Add the blood sample to be tested and the reagents required for the test into the reaction pool 30 to form a mixed sample solution;

[0078] S20. Mix the mixed sample solution;

[0079] S30. Extract the mixed sample solution located in the middle of the reaction pool 30 and temporarily store it in the buffer channel 61;

[0080] S40. Detect the mixed sample solution buffered in the buffer channel 61.

[0081] Specifically, through the setting of the above-mentioned specific protein analyzer 100, in step S10, the blood sample to be tested, the first reagent, and the second reagent can be added into the reaction pool 30 through the sample supply device 80, the first reagent supply device 10, and the second reagent supply device 20 respectively. Then, the mixed sample solution is mixed by the mixing component 50, and the mixed sample solution located in the middle of the reaction pool 30 is extracted into the buffer channel 61 by the buffer component 60. Finally, specific protein analysis and detection are performed on this part of the mixed sample solution with higher quality, and more accurate detection results can be obtained.

[0082] For one embodiment, please refer to Figure 7, corresponding to the solution implemented by the mixing component 50 using the suction and discharge mixing method, the mixing component 50 includes a mixing channel 51 and a mixing power device 52. The mixing channel 51 includes a first end 511 and a second end 512, and the mixing channel 51 is filled with liquid. The first end 511 of the mixing channel 51 communicates with the inner cavity 301 of the reaction tank 30, and the second end 512 of the mixing channel 51 is connected to the mixing power device 52. In step S20 "mix the mixed sample liquid", the following steps are further included:

[0083] S21. The mixing power device 52 performs at least one of the following suction and push mixing operations:

[0084] S211. Drive the mixed sample liquid in the reaction tank 30 to flow away from the reaction tank 30, so as to suck the mixed sample liquid in the reaction tank 30 into the mixing channel 51 communicating with the reaction tank 30;

[0085] S212. Then drive the liquid in the mixing channel 51 to flow towards the reaction tank 30, so as to push the mixed sample liquid in the mixing channel 51 into the reaction tank 30, thereby forming a swirl in the reaction tank 30 to mix the mixed sample liquid.

[0086] Specifically, in this step, by performing at least one suction and push mixing operation by the mixing power device 52, the effect of mixing most of the mixed sample liquid in the reaction tank 30 can be achieved. It should be noted that when the mixing power device 52 mixes the mixed sample liquid in the reaction tank 30, it does not need to suck all the mixed sample liquid into the mixing channel 51, but only needs to suck a part of the mixed sample liquid and push it back into the reaction tank 30 to stir the remaining mixed sample liquid in the reaction tank 30 that has not been sucked out, so as to achieve the effect of mixing the mixed sample liquid in the reaction tank 30. Therefore, please refer to Figure 8 In the embodiment of, when in step S21 "the mixing power device 52 performs at least one of the following suction and push mixing operations", the following sub-steps can further be included:

[0087] S211a. Drive at least half of the capacity of the mixed sample liquid or the primary mixed sample liquid in the reaction tank 30 to flow away from the reaction tank 30, so as to suck the mixed sample liquid or the primary mixed sample liquid in the reaction tank 30 into the mixing channel 51 communicating with the reaction tank 30;

[0088] S212a. Then drive the liquid in the mixing channel 51 to flow towards the reaction tank 30, so as to push the mixed sample liquid or the primary mixed sample liquid in the mixing channel 51 into the reaction tank 30, thereby forming a swirl in the reaction tank 30 to mix the mixed sample liquid or the primary mixed sample liquid.

[0089] Specifically, the initial mixed sample liquid in this embodiment can be a liquid formed by mixing a blood sample to be tested and a first reagent. For detailed information, please refer to the relevant descriptions in the subsequent embodiments. In this embodiment, by sucking at least half of the volume of the mixed sample liquid or the initial mixed sample liquid in the reaction cell 30 into the mixing channel 51 and then pushing it back into the reaction cell 30, it can ensure that most of the mixed sample liquid or the initial mixed sample liquid in the reaction cell 30 is effectively mixed.

[0090] In one embodiment, the number of suction-push mixing operations of the mixing power device 52 in step S21, "the mixing power device 52 performs at least one suction-push mixing operation as follows", is two.

[0091] For one embodiment, please refer to Figure 9 , in step S10, "adding a blood sample to be tested and a reagent required for testing into the reaction cell 30 to form a mixed sample liquid", it further includes:

[0092] S11. Adding a first reagent and a sample to be tested into the reaction cell 30 to form an initial mixed sample liquid;

[0093] S12. Mixing the initial mixed sample liquid;

[0094] S13. The buffering power device 62 drives the initial mixed sample liquid located in the middle of the reaction cell 30 to flow into the buffering channel 61 and temporarily stores it in the buffering channel 61;

[0095] S14. Emptying the remaining initial mixed sample liquid in the reaction cell 30;

[0096] S15. The buffering power device 62 pushes the initial mixed sample liquid in the mixing channel 51 back into the reaction cell 30;

[0097] S16. Adding a second reagent into the reaction cell 30, and the second reagent is mixed with the initial mixed sample liquid in the reaction cell 30 to form a mixed sample liquid.

[0098] Specifically, in this embodiment, it is necessary to first prepare Figure 8 the initial mixed sample liquid mentioned in the embodiment. When the first reagent is a hemolytic agent or other reagent used to catalyze the dissolution of the blood sample to be tested, the initial mixed sample liquid formed by mixing the first reagent and the blood sample to be tested can be first mixed to make the blood sample to be tested fully dissolve. Then, based on a principle similar to the above-mentioned mixed sample liquid, the buffering component 60 stores a part of the initial mixed sample liquid with better mixing quality located in the middle of the reaction cell 30 into the buffering channel 61, and empties the remaining part of the initial mixed sample liquid with relatively poor mixing effect in the reaction cell 30. Finally, the fully mixed mixed sample liquid is sent back into the reaction cell 30 and mixed with the second reagent, so that the mixing quality of the obtained mixed sample liquid can be further improved.

[0099] It can be understood. Please refer to Figure 10 the embodiments shown. When performing "mixing the primary mixed sample liquid" in step S12, the following steps may be included:

[0100] S121. The mixing power device 52 performs at least one of the following suction and push mixing operations:

[0101] S1211. Drive the primary mixed sample liquid in the reaction cell 30 to flow away from the reaction cell 30, so as to suck the primary mixed sample liquid in the reaction cell 30 into the mixing channel 51 communicating with the reaction cell 30;

[0102] S1212. Then drive the liquid in the mixing channel 51 to flow towards the reaction cell 30, so as to push the primary mixed sample liquid in the mixing channel 51 into the reaction cell 30, thereby forming a swirl in the reaction cell 30 to mix the primary mixed sample liquid.

[0103] For an embodiment, please refer to Figure 11 , corresponding to the embodiment where the reaction cell 30 is configured as the detection area 71. After step S30 "extracting the mixed sample liquid located in the middle of the reaction cell 30 and temporarily storing it in the buffer channel 61", it further includes:

[0104] S35. Empty the remaining mixed sample liquid in the reaction cell 30;

[0105] S36. The buffer power device 62 pushes the mixed sample liquid in the buffer channel 61 back into the reaction cell 30;

[0106] Then, step S40 "detecting the mixed sample liquid buffered in the buffer channel 61" includes:

[0107] S40a. The detection device 70 performs a specific protein concentration detection on the mixed sample liquid in the reaction cell 30.

[0108] Specifically, in this embodiment, by emptying the remaining relatively poor-quality mixed sample liquid in the reaction cell 30 and then pushing the relatively high-quality mixed sample liquid temporarily stored in the buffer channel 61 back into the reaction cell 30 for detection, the influence of the remaining mixed sample liquid in the reaction cell 30 on the detection result can be avoided, ensuring the detection accuracy of the specific protein determination method of the present application.

[0109] For an embodiment, after step S36 "the buffer power device 62 pushes the mixed sample liquid in the buffer channel 61 back into the reaction cell 30", it further includes:

[0110] S37. Mix the mixed sample liquid in the reaction cell 30.

[0111] Specifically, before detecting the specific protein in the mixed sample solution temporarily stored in the buffer channel 61, the mixed sample solution can be mixed again by the mixing component 50, or called secondary mixing, to improve the detection accuracy.

[0112] In one embodiment, corresponding to the embodiment in which the specific protein analyzer 100 is provided with a preheating device 92, after step S30 "extracting the mixed sample solution located in the middle of the reaction cell 30 and temporarily storing it in the buffer channel 61", it further includes:

[0113] S32. The preheating device 92 heats the mixed sample solution temporarily stored in the buffer channel 61 to a preset temperature.

[0114] Specifically, by heating the mixed sample solution in the buffer channel 61 through the preheating device 92, the mixed sample solution can reach the preset temperature before detection, thereby improving the detection consistency of the specific protein determination method of the present application.

[0115] In one embodiment, when applying the specific protein determination method of the present application, the mixing channel 51 and the buffer channel 61 are integrally provided, and the mixing power device 52 and the buffer power device 62 are integrally provided to reduce the number of components used when implementing this method.

[0116] In one embodiment, in step S30 "extracting the mixed sample solution located in the middle of the reaction cell 30 and temporarily storing it in the buffer channel 61", it further includes:

[0117] S30a. The buffer power device 62 drives the mixed sample solution or the primary mixed sample solution located in the middle of the reaction cell 30 and occupying at least 3 / 4 of the capacity of the reaction cell 30 to flow into the buffer channel 61 and be temporarily stored in the buffer channel 61.

[0118] Specifically, that is, after the mixing operation by the mixing component 50, in order to ensure that the volume of the mixed sample solution for specific protein detection is sufficient, the buffer power device 62 is also controlled to extract and temporarily store at least 3 / 4 of the mixed sample solution in the reaction cell 30, and perform specific protein detection on at least 3 / 4 of the mixed sample solution subsequently to ensure the validity of the detection data.

[0119] Please refer to Figure 12 A computer-readable storage medium 400 provided in the third aspect of the present application as shown, including a storage device 402 storing executable program instructions, and configured such that when the processor 401 executes the executable program instructions, it implements the specific protein determination method provided in the second aspect of the present application.

[0120] Specifically, in one embodiment, the processor 401 calls the program instructions stored in the storage device 402 and performs the following operations:

[0121] Add a blood sample to be tested and the reagents required for testing into the reaction pool 30 to form a mixed sample solution;

[0122] Mix the mixed sample solution;

[0123] Extract the mixed sample solution located in the middle of the reaction pool 30 and temporarily store it in the buffer channel 61;

[0124] Detect the mixed sample solution buffered in the buffer channel 61.

[0125] The storage device 402 may include a volatile memory, such as a random-access memory (RAM); the storage device 402 may also include a non-volatile memory, such as a flash memory, a solid-state drive (SSD), etc.; the storage device 402 may further include a combination of the above types of storage devices.

[0126] The processor 401 may be a central processing unit (CPU). The processor 401 may also be other general-purpose processors, digital signal processors (DSPs), application specific integrated circuits (ASICs), field-programmable gate arrays (FPGAs), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor may be a microprocessor or the processor may also be any conventional processor, etc.

[0127] It should be noted that in the second and third aspects of the present application, since the principles applied are similar to the principle of the specific protein analyzer 100 in the first aspect of the present application, that is, by detecting the liquid located in the middle of the reaction pool 30 extracted from the mixed sample solution after mixing to ensure the accuracy of the specific protein. Therefore, the development of each embodiment in the above two aspects can also be based on the embodiments of the specific protein analyzer 100 in the first aspect of the present application, and will not be elaborated one by one in this specification.

[0128] The above-described embodiments do not constitute a limitation on the protection scope of the technical solution. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the above embodiments shall be included in the protection scope of the technical solution.

Claims

1. A specific protein analyzer, characterized in that it includes: A sample supply device for providing a blood sample to be tested; A first reagent supply device for providing a first reagent that reacts with the blood sample to be tested; A second reagent supply device for providing a second reagent that reacts with the blood sample to be tested; A reaction cell that receives the blood sample to be tested supplied by the sample supply device, the first reagent supplied by the first reagent supply device, and the second reagent supplied by the second reagent supply device, so that the blood sample to be tested reacts with the first reagent and the second reagent to form a mixed sample solution; A mixing assembly for mixing the mixed sample solution in the reaction cell; A buffer assembly, including a buffer channel and a buffer power device, the buffer channel includes a first end and a second end and is filled with liquid, the first end of the buffer channel communicates with the inner cavity of the reaction cell, and the second end of the buffer channel is connected to the buffer power device; A detection device, including a detection area made of a light-transmitting material and a light source corresponding to the detection area, the light source is used to irradiate the mixed sample solution in the detection area to detect the content of specific protein in the mixed sample solution; A control device configured to: Control the buffer power device to drive the liquid in the buffer channel to flow away from the reaction cell, suck out the mixed sample solution located in the middle of the reaction cell into the buffer channel for buffering, for detecting the content of specific protein.

2. The specific protein analyzer according to claim 1, wherein The mixing assembly includes a mixing channel and a mixing power device, the mixing channel includes a first end and a second end and is filled with liquid, the first end of the mixing channel communicates with the inner cavity of the reaction cell, and the second end of the mixing channel is connected to the mixing power device; The control device is further configured to: Control the mixing power device to drive the liquid in the mixing channel to flow away from the reaction cell or towards the reaction cell, so as to suck out the liquid in the reaction cell into the mixing channel or push the liquid in the mixing channel into the reaction cell.

3. The specific protein analyzer according to claim 1 or 2, characterized in that, The reaction cell includes a detection area made of a light-transmitting material, and the detection device is correspondingly arranged with the detection area; The control device is further configured to: Control the buffer power device to drive the liquid in the buffer channel to flow away from the reaction cell, suck out the mixed sample solution located in the middle of the reaction cell into the buffer channel for buffering; Control the remaining mixed sample solution in the reaction cell to be discharged out of the reaction cell; Control the buffer power device to drive the liquid in the buffer channel to flow towards the reaction cell, so as to push the mixed sample solution in the buffer channel into the reaction cell; Control the detection device to detect the concentration of specific protein in the mixed sample solution in the reaction cell.

4. The specific protein analyzer according to claim 1, wherein The buffer channel includes a detection area made of a light-transmitting material, and the detection device is correspondingly arranged with the detection area; The control device is further configured to: Control the buffer power device to drive the liquid in the buffer channel to flow away from the reaction pool, so as to suck out the mixed sample liquid in the middle of the reaction pool into the buffer channel for buffering; Control the detection device to detect the specific protein concentration of the mixed sample liquid in the buffer channel.

5. The specific protein analyzer according to claim 1, wherein It further includes a preheating device for heating the liquid. The preheating device is arranged in the buffer channel to heat the liquid in the buffer channel.

6. The specific protein analyzer according to claim 5, wherein, The preheating device is a heating rod, and the buffer channel is spirally wound around the outside of the heating rod.

7. The specific protein analyzer according to claim 2, characterized in that, It further includes a cleaning liquid supply device for providing cleaning liquid to clean the liquid path. The cleaning liquid supply device is communicated with the mixing channel and the buffer channel.

8. The specific protein analyzer according to claim 2, wherein The mixing channel and the buffer channel are integrally arranged, and the mixing power device and the buffer power device are integrally arranged.

9. The specific protein analyzer according to claim 8, characterized in that, A side inlet communicating with its inner cavity is opened on the side wall of the reaction pool, and a waste liquid discharge port communicating with its inner cavity is opened at the bottom of the reaction pool. The side inlet is used to connect the first reagent supply device and the first end of the mixing channel.

10. The specific protein analyzer according to claim 9, wherein The side inlet includes a first side inlet and a second side inlet. The first side inlet is communicated with the first reagent supply device, and the second side inlet is communicated with the first end of the mixing channel.

11. A method for determining a specific protein, characterized in that, Applied to the specific protein analyzer according to any one of claims 1 to 10, it includes the following steps: Add a blood sample to be tested and a reagent required for testing into the reaction pool to form a mixed sample liquid; Mix the mixed sample liquid; Extract the mixed sample liquid in the middle of the reaction pool and temporarily store it in the buffer channel; Detect the mixed sample liquid buffered in the buffer channel.

12. The specific protein determination method according to claim 11, wherein The mixing assembly includes a mixing channel and a mixing power device. The mixing channel includes a first end and a second end and is filled with liquid. The first end of the mixing channel is communicated with the inner cavity of the reaction pool, and the second end of the mixing channel is connected to the mixing power device; The step of mixing the mixed sample liquid includes the following steps: The mixing power device performs at least one of the following suction-push mixing operations: Drive the mixed sample liquid in the reaction pool to flow away from the reaction pool, so as to suck out the mixed sample liquid in the reaction pool into the mixing channel communicated with the reaction pool; Then drive the liquid in the mixing channel to flow towards the reaction pool, so as to push the mixed sample liquid in the mixing channel into the reaction pool, thereby forming a swirl in the reaction pool to mix the mixed sample liquid.

13. The specific protein determination method according to claim 11, wherein, The step of adding a blood sample to be tested and a reagent required for testing into the reaction pool to form a mixed sample liquid specifically includes: Add a first reagent and a sample to be tested into the reaction pool to form a primary mixed sample liquid; Mix the primary mixed sample liquid; The buffer power device drives the primary mixed sample liquid in the middle of the reaction pool to flow into the buffer channel and temporarily store it in the buffer channel; Empty the remaining primary mixed sample liquid in the reaction pool; The buffer power device pushes the primary mixed sample liquid in the mixing channel back into the reaction pool; Add a second reagent into the reaction cell, and the second reagent is mixed with the initially mixed sample in the reaction cell to form the mixed sample solution.

14. The specific protein determination method according to claim 13, wherein, Mix the initially mixed sample solution, including the following steps: The mixing power device performs at least one of the following suction-push mixing operations: Drive the initially mixed sample solution in the reaction cell to flow away from the reaction cell, so as to suck the initially mixed sample solution in the reaction cell into the mixing channel communicated with the reaction cell; Then drive the liquid in the mixing channel to flow towards the reaction cell, so as to push the initially mixed sample solution in the mixing channel into the reaction cell, thereby forming a vortex in the reaction cell to mix the initially mixed sample solution.

15. The specific protein determination method according to claim 11, wherein After extracting the mixed sample solution located in the middle of the reaction cell and temporarily storing it in the buffer channel, it further includes: Empty the remaining mixed sample solution in the reaction cell; The buffer power device pushes the mixed sample solution in the buffer channel back into the reaction cell; Then, detecting the mixed sample solution buffered in the buffer channel includes: The detecting device detects the specific protein concentration of the mixed sample solution in the reaction cell.

16. The specific protein determination method according to claim 15, characterized in that, After the buffer power device pushes the mixed sample solution in the buffer channel back into the reaction cell, it further includes: Mix the mixed sample solution in the reaction cell.

17. The specific protein determination method according to claim 11, wherein After extracting the mixed sample solution located in the middle of the reaction cell and temporarily storing it in the buffer channel, it further includes; The preheating device heats the mixed sample solution temporarily stored in the buffer channel to a preset temperature.

18. The specific protein determination method according to claim 12, characterized in that, The mixing channel and the buffer channel are integrally arranged, and the mixing power device and the buffer power device are integrally arranged.

19. The specific protein determination method according to claim 12, wherein When the mixing power device performs at least one of the suction-push mixing operations, it specifically includes: Drive at least half of the capacity of the mixed sample solution or the initially mixed sample solution in the reaction cell to flow away from the reaction cell, so as to suck the mixed sample solution or the initially mixed sample solution in the reaction cell into the mixing channel communicated with the reaction cell; Then drive the liquid in the mixing channel to flow towards the reaction cell, so as to push the mixed sample solution or the initially mixed sample solution in the mixing channel into the reaction cell, thereby forming a vortex in the reaction cell to mix the mixed sample solution or the initially mixed sample solution.

20. The specific protein determination method according to claim 12, wherein Extracting the mixed sample solution located in the middle of the reaction cell and temporarily storing it in the buffer channel specifically includes: The buffer power device drives the mixed sample solution or the initially mixed sample solution located in the middle of the reaction cell and occupying at least 3 / 4 of the capacity of the reaction cell to flow into the buffer channel and temporarily store it in the buffer channel.

21. A computer-readable storage medium stores executable instructions configured to cause a processor to implement the specific protein determination method according to any one of claims 11 to 20 when executing the executable instructions.

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