Tissue culture method of origanum cordata JackFrost

Abstract

The invention relates to the technical field of plant in-vitro culture, and particularly discloses a tissue culture method of origanum cordata Jack Frost. The method specifically comprises the following steps: obtaining and disinfecting an explant, inducing the explant to form a callus and an adventitious bud, proliferating the adventitious bud, and inducing the adventitious bud to root, wherein in the step of obtaining and disinfecting the explants, new leaves of stock plants which grow robustly and are free of plant diseases and insect pests are selected as the explants; the invention also provides a culture medium formula for inducing the explants to form calluses and adventitious buds, proliferating the adventitious buds and inducing the adventitious buds to root. By means of the invention, the breeding efficiency of the origanum cordata Jack Frost can be effectively improved, and application of the origanum cordata Jack Frost is promoted.

Description

technical field

[0001] The present application relates to the technical field of plant in vitro culture, more specifically, it relates to a tissue culture method of Buglossia cordata JackFrost. Background technique

[0002] Jack Frost (Brunnera macrophyllaJack Frost), belonging to the genus Bugloss in the family Boraginaceae, is a perennial herb, clustered and hemispherical; the leaves are oval and heart-shaped, and the leaf color is frosty silver with green veins ; Leaf surface and leaf back densely covered with white pilose. The plants of this variety have high ornamental value, and are suitable for foreground arrangement of flower borders in shaded areas, stream and water feature planting, roadside cluster planting, private garden planting and potted ornamental planting. It has a wide range of applications and has a good market prospect.

[0003] Jack Frost likes a cool and humid climate, likes loose and well-drained sandy loam, tolerates half shade, and grows well under...

Examples

Embodiment 1

[0061] The present embodiment provides a kind of tissue culture method of Buglossia cordata Jack Frost, specifically comprises the following steps:

[0062] 1. Acquisition and Sterilization of Explants

[0063] (1) Acquisition of explants: place the mother plant in the greenhouse and cultivate it for 35 days, remove dead and diseased leaves in the process, and spray a preventive fungicide every 15 days; For the mother plant, select new leaves that are fully expanded and free from diseases and insect pests on the mother plant;

[0064] (2) Disinfection of explants:

[0065] Rinsing: Rinse the new leaves obtained in step (1) under flowing tap water for 10 minutes, and dry them;

[0066] Alcohol disinfection: After drying, soak the new leaves in 75% alcohol for 30 seconds, then wash them with sterile water for 1-2 minutes each time;

[0067] 0.1% mercuric chloride treatment: put the new leaves in 0.1% mercuric chloride solution, soak and shake for 5 minutes, and then wash with...

Embodiment 2-3

[0085] Embodiment 2-3 provides a kind of tissue culture method of Buglossia cordata Jack Frost respectively, and its difference with embodiment 1 is the parameter in each step and the culture medium formula used, specifically as shown in table 1 Show.

Embodiment 4-5

[0090] Embodiment 4-5 provides a kind of tissue culture method of Buglossia cordata Jack Frost respectively, and its difference with embodiment 2 is the growth period of the new leaves as explants on the mother plant, as shown in the table 2.

technical field

[0001] The present application relates to the technical field of plant in vitro culture, more specifically, it relates to a tissue culture method of Buglossia cordata JackFrost. Background technique

[0002] Jack Frost (Brunnera macrophyllaJack Frost), belonging to the genus Bugloss in the family Boraginaceae, is a perennial herb, clustered and hemispherical; the leaves are oval and heart-shaped, and the leaf color is frosty silver with green veins ; Leaf surface and leaf back densely covered with white pilose. The plants of this variety have high ornamental value, and are suitable for foreground arrangement of flower borders in shaded areas, stream and water feature planting, roadside cluster planting, private garden planting and potted ornamental planting. It has a wide range of applications and has a good market prospect.

[0003] Jack Frost likes a cool and humid climate, likes loose and well-drained sandy loam, tolerates half shade, and grows well under...

Examples

Embodiment 1

[0061] The present embodiment provides a kind of tissue culture method of Buglossia cordata Jack Frost, specifically comprises the following steps:

[0062] 1. Acquisition and Sterilization of Explants

[0063] (1) Acquisition of explants: place the mother plant in the greenhouse and cultivate it for 35 days, remove dead and diseased leaves in the process, and spray a preventive fungicide every 15 days; For the mother plant, select new leaves that are fully expanded and free from diseases and insect pests on the mother plant;

[0064] (2) Disinfection of explants:

[0065] Rinsing: Rinse the new leaves obtained in step (1) under flowing tap water for 10 minutes, and dry them;

[0066] Alcohol disinfection: After drying, soak the new leaves in 75% alcohol for 30 seconds, then wash them with sterile water for 1-2 minutes each time;

[0067] 0.1% mercuric chloride treatment: put the new leaves in 0.1% mercuric chloride solution, soak and shake for 5 minutes, and then wash with...

Embodiment 2-3

[0085] Embodiment 2-3 provides a kind of tissue culture method of Buglossia cordata Jack Frost respectively, and its difference with embodiment 1 is the parameter in each step and the culture medium formula used, specifically as shown in table 1 Show.

Embodiment 4-5

[0090] Embodiment 4-5 provides a kind of tissue culture method of Buglossia cordata Jack Frost respectively, and its difference with embodiment 2 is the growth period of the new leaves as explants on the mother plant, as shown in the table 2.

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