A cell constant temperature culture device

Abstract

The present invention discloses a cell constant temperature culture device, including an incubator, characterized in that: a mounting box is fixedly connected to one side of the incubator, a temperature-adjusting water tank is fixedly connected to one side of the mounting box, a heating component and a refrigeration component are arranged on the temperature-adjusting water tank, and a stirring mechanism is arranged in the temperature-adjusting water tank; a first heat exchange water pipe is arranged in a spiral shape in the temperature-adjusting water tank, a water pump is arranged in the mounting box, one end of the first heat exchange water pipe is connected to the water inlet of the water pump, the water outlet of the water pump is connected to the second heat exchange water pipe, the second heat exchange water pipe is plugged into the incubator, and the second heat exchange water pipe is continuously bent and arranged on the side wall of the incubator. The present invention uses water as a heat transfer medium to achieve smooth temperature regulation of the cell culture device, so that the temperature change of the cell culture device is extremely small, and it is easier to achieve a long-term stable constant temperature state.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture equipment, in particular to a cell constant temperature culture device. Background Art

[0002] During plant cell culture, callus tissue or other easily dispersed tissue is placed in a liquid culture medium for shaking culture under in vitro conditions to obtain dispersed free suspension cells, and the cells are multiplied by subculture to obtain a large number of cell populations. Plant cell culture mainly includes several steps such as separation of single cells, cell culture and cell preservation. The methods for separating single cells mainly include physical methods, chemical methods and enzymatic methods. The physical method is to place loose callus tissue in a liquid culture medium and continuously vibrate the shaker to disperse the cells; the chemical method is to add oxalate (calcium ions that can bind to pectin calcium in the intercellular matrix), colchicine, 2,4-D or hydrolyzed milk protein to the cell suspension culture; the enzymatic method is to use pectinase and cellulase to separate cells. The methods of cell culture mainly include: plate culture, nursing culture, liquid shallow culture, microchamber culture and suspension cell culture. Cell preservation includes subculture preservation, low temperature preservation (culture at 5-10 degrees and regular replacement of culture medium) and frozen preservation. Frozen preservation is further preserved by low temperature preservation and ultra-low temperature preservation.

[0003] Cell culture generally requires a constant temperature environment, so cell culture is generally carried out using a constant temperature culture device. Currently, most cell constant temperature culture devices directly use heating plates and cooling plates to perform temperature increase and decrease operations. The temperature changes of this type of temperature increase and decrease operation are relatively unstable. The temperature control method of direct heating or passing cold air will cause the temperature to change extremely quickly, and it is impossible to adjust the temperature gently. In addition, the temperature control needs to be stopped after heating or cooling for a certain period of time. When the ambient temperature of the cell culture becomes abnormal again, heating or cooling is turned on again. Such repeated rapid temperature changes will cause cell death, thereby reducing the practicality of the cell constant temperature culture device. In addition, in general, cell culture needs to be carried out under sterile conditions, and air will be passed into the constant temperature device, thereby affecting the sterile environment inside the constant temperature device. When air is passed in, it is also easy to cause rapid temperature changes, thereby affecting the cultivation of plant cells. Therefore, we need to propose a cell constant temperature culture device. Summary of the invention

[0004] The object of the present invention is to provide a cell constant temperature culture device, which uses water as a heat transfer medium. Through the pre-temperature adjustment of a temperature-adjusting water tank, the water and the internal space of the culture box are heat exchanged through heat exchange tubes, thereby achieving smooth temperature adjustment of the cell culture device, making the temperature change of the cell culture device extremely small, and making it easier to achieve a long-term stable constant temperature state, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a cell constant temperature culture device, comprising an incubator, a mounting box is fixedly connected to one side of the incubator, a temperature regulating water tank is fixedly connected to one side of the mounting box, a heating component and a cooling component are arranged on the temperature regulating water tank, and a stirring mechanism is arranged in the temperature regulating water tank;

[0006] A first heat exchange water pipe is arranged in the temperature regulating water tank, the first heat exchange water pipe is arranged in a spiral shape, a water pump is arranged in the installation box, one end of the first heat exchange water pipe is connected to the water inlet of the water pump, the water outlet of the water pump is connected to the second heat exchange water pipe, the second heat exchange water pipe is plugged into the incubator, and the second heat exchange water pipe is continuously bent and arranged on the side wall of the incubator, one end of the second heat exchange water pipe is connected to one end of the first heat exchange water pipe, an inner liner is arranged in the incubator, and the second heat exchange water pipe is located between the inner wall of the incubator and the inner liner;

[0007] A temperature monitoring sensor is fixedly installed in the temperature regulating water tank, and a temperature controller is fixedly installed on the outer wall of the temperature regulating water tank. The temperature controller is electrically connected to the temperature monitoring sensor, the heating component, the refrigeration component and the stirring mechanism.

[0008] Preferably, two groups of partitions are provided in the temperature-controlled water tank, and the two groups of partitions are arranged in an L shape. The temperature-controlled water tank is divided into a heating chamber, a cooling chamber and a mixing chamber by the two groups of partitions. The heating component is located in the heating chamber, the cooling component is located in the cooling chamber, the stirring mechanism is located in the mixing chamber, and the partitions are provided with water-permeable holes.

[0009] Preferably, the heating assembly comprises an electric heating plate, a first heat conducting plate is provided at the lower end of the electric heating plate, and the first heat conducting plate extends into the heating cavity.

[0010] Preferably, the refrigeration component includes a semiconductor refrigeration plate, the cold end of the semiconductor refrigeration plate is fixedly connected to a second heat conduction plate, and one end of the second heat conduction plate extends into the refrigeration cavity; the refrigeration component also includes a radiator, which is fixedly connected to the hot end of the semiconductor refrigeration plate, and the radiator is configured as a tower radiator, and fans are fixedly installed at both ends of the radiator.

[0011] Preferably, the stirring mechanism comprises a stirring rod, on which a stirring blade is provided, the stirring rod is located between two groups of partitions, the water-permeable holes on the two groups of partitions are opened on opposite side walls of the two groups of partitions, and the lower end of the stirring rod extends close to the first water exchange water pipe;

[0012] The stirring mechanism also includes a motor, which is fixedly installed on the upper end of the temperature-controlled water tank. One end of the motor output shaft is inserted into the temperature-controlled water tank, and one end of the motor output shaft is fixedly connected to the upper end of the stirring rod.

[0013] Preferably, a ventilation box is fixedly mounted on the upper end of the installation box, one end of the ventilation box is plugged into the inner liner, and the ventilation box is connected to the inner liner, and an air inlet is opened on the upper end of the ventilation box;

[0014] The second water exchange heat pipe passes through the ventilation box, and the portion of the second water exchange heat pipe located in the ventilation box is bent in a circuitous manner.

[0015] Preferably, a ventilation mechanism is embedded and fixed in the air inlet, and a filter is detachably connected to the upper end of the ventilation mechanism, and the filter and the ventilation mechanism are sealed.

[0016] Preferably, the ventilation mechanism includes a connecting plate, a countersunk through hole is opened on the connecting plate, an internal thread is provided at the upper end of the countersunk through hole, the filter element is threadedly installed on the upper end of the countersunk through hole, a sealing ring is provided in the countersunk through hole, the lower end of the filter element is tightly fitted with the sealing ring, and a ventilation fan is embedded in the lower end of the countersunk through hole.

[0017] Preferably, the filter element comprises a tube body, an annular flange is arranged in the tube body, a filter core is arranged at the upper end of the annular flange, a magnet block is fixedly mounted at the lower end of the filter core, the annular flange is arranged as an iron annular flange, and the magnet block is attracted to the annular flange.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. The present invention uses water as a heat transfer medium through the design of a temperature-adjusting water tank, a first heat exchange water pipe and a second heat exchange water pipe. The water is adjusted to a suitable temperature through the pre-temperature adjustment of the temperature-adjusting water tank, and then heat is exchanged with the water through the first heat exchange water pipe. The first heat exchange water pipe is then connected to the second heat exchange water pipe. The second heat exchange water pipe is bent and laid in the incubator, so that the second heat exchange water pipe exchanges heat with the internal space of the incubator, thereby realizing smooth adjustment of the temperature of the cell culture device, making the temperature change of the cell culture device extremely small, and making it easier to achieve a long-term stable constant temperature state;

[0020] 2. The present invention connects one end of the ventilation box to the inner tank through plugging, an air inlet is opened at the upper end of the ventilation box, a filter is arranged in the air inlet, and the second heat exchange water pipe is bent and runs through the ventilation box, so that when air is passed into the constant temperature device, it can be filtered through the filter first, thereby avoiding the environment inside the constant temperature device from being affected by the air, and when the air is passed, the air is pre-heated through the second heat exchange water pipe, avoiding the rapid temperature change from affecting the cultivation of plant cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a structural schematic diagram of the present invention;

[0022] Figure 2 is a schematic diagram of a water pump of the present invention;

[0023] Figure 3 is a schematic diagram of a first water exchange pipe of the present invention;

[0024] Figure 4 is a schematic diagram of the ventilation mechanism of the present invention;

[0025] Figure 5 Schematic diagram of the filter element of the present invention.

[0026] In the figure: 1. incubator; 2. ventilation box; 3. temperature-adjusting water tank; 4. installation box; 5. heating assembly; 6. refrigeration assembly; 601. semiconductor refrigeration sheet; 602. radiator; 603. fan; 7. stirring mechanism; 701. stirring rod; 702. motor; 8. first heat exchange water pipe; 9. filter element; 901. pipe body; 902. annular flange; 903. filter element; 904. magnet block; 10. second heat exchange water pipe; 11. liner; 12. water pump; 13. ventilation mechanism; 1301. connecting plate; 1302. ventilation fan; 1303. sealing ring; 14. temperature monitoring sensor; 15. temperature controller; 16. partition; 17. first heat conduction plate; 18. second heat conduction plate. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] See also Figure 1-5 The present invention provides a technical solution: a cell constant temperature culture device, comprising an incubator 1, a mounting box 4 is fixedly connected to one side of the incubator 1, a temperature regulating water tank 3 is fixedly connected to one side of the mounting box 4, a heating component 5 and a refrigeration component 6 are arranged on the temperature regulating water tank 3, and a stirring mechanism 7 is arranged in the temperature regulating water tank 3;

[0029] A first heat exchange water pipe 8 is provided in the temperature regulating water tank 3, and the first heat exchange water pipe 8 is provided in a spiral shape. A water pump 12 is provided in the installation box 4, and one end of the first heat exchange water pipe 8 is connected to the water inlet of the water pump 12, and the water outlet of the water pump 12 is connected to the second heat exchange water pipe 10, and the second heat exchange water pipe 10 is plugged into the incubator 1, and the second heat exchange water pipe 10 is continuously bent and arranged on the side wall of the incubator 1, and one end of the second heat exchange water pipe 10 is connected to one end of the first heat exchange water pipe 8, and an inner liner 11 is provided in the incubator 1, and the second heat exchange water pipe 10 is located between the inner wall of the incubator 1 and the inner liner 11;

[0030] A temperature monitoring sensor 14 is fixedly installed in the temperature regulating water tank 3 , and a temperature controller 15 is fixedly installed on the outer wall of the temperature regulating water tank 3 . The temperature controller 15 is electrically connected to the temperature monitoring sensor 14 , the heating component 5 , the refrigeration component 6 and the stirring mechanism 7 .

[0031] Specifically, the temperature monitoring sensor 14 continuously monitors the water temperature. When the water temperature is too high, the temperature controller 15 receives a signal to start the refrigeration component 6 and the stirring mechanism 7 to cool the water in the temperature-controlled water tank 3; when the water temperature is too low, the temperature controller 15 receives a signal to start the heating component 5 and the stirring mechanism 7 to cool the water in the temperature-controlled water tank 3;

[0032] Two groups of partitions 16 are arranged in the temperature-adjusting water tank 3, and the two groups of partitions 16 are arranged in an L shape. The temperature-adjusting water tank 3 is divided into a heating chamber, a cooling chamber and a mixing chamber by the two groups of partitions 16. The heating component 5 is located in the heating chamber, the cooling component 6 is located in the cooling chamber, and the stirring mechanism 7 is located in the mixing chamber. The partitions 16 are provided with water-permeable holes;

[0033] By setting the partition 16, when the temperature-adjusting water tank 3 adjusts the water temperature, the heating component 5 or the cooling component 6 can first heat the local water temperature, and then balance the water temperature through the penetration of the water-permeable holes and the mixing of the stirring mechanism 7, so that the change of the water temperature is more gentle;

[0034] The heating assembly 5 includes an electric heating plate, a first heat conducting plate 17 is provided at the lower end of the electric heating plate, and the first heat conducting plate 17 extends into the heating cavity; the refrigeration assembly 6 includes a semiconductor refrigeration plate 601, a second heat conducting plate 18 is fixedly connected to the cold end of the semiconductor refrigeration plate 601, and one end of the second heat conducting plate 18 extends into the refrigeration cavity; the refrigeration assembly 6 also includes a radiator 602, the radiator 602 is fixedly connected to the hot end of the semiconductor refrigeration plate 601, the radiator 602 is configured as a tower radiator, and fans 603 are fixedly installed at both ends of the radiator 602;

[0035] The design of the first heat conducting plate 17 and the second heat conducting plate 18 enables the heating component 5 and the cooling component 6 to better exchange heat with water;

[0036] The stirring mechanism 7 includes a stirring rod 701, on which a stirring blade is provided, the stirring rod 701 is located between the two groups of partitions 16, the water-permeable holes on the two groups of partitions 16 are opened on the opposite side walls of the two groups of partitions 16, and the lower end of the stirring rod 701 extends close to the first water exchange pipe 8; the stirring mechanism 7 also includes a motor 702, which is fixedly installed at the upper end of the temperature regulating water tank 3, one end of the output shaft of the motor 702 is inserted into the temperature regulating water tank 3, and one end of the output shaft of the motor 702 is fixedly connected to the upper end of the stirring rod 701;

[0037] A ventilation box 2 is fixedly installed on the upper end of the installation box 4, one end of the ventilation box 2 is inserted into the inner tank 11, and the ventilation box 2 is communicated with the inner tank 11, and an air inlet is provided at the upper end of the ventilation box 2; the second hot water exchange pipe 10 runs through the ventilation box 2, and the part of the second hot water exchange pipe 10 located in the ventilation box 2 is arranged in a circuitous manner, and a ventilation mechanism 13 is embedded and fixed in the air inlet, and a filter element 9 is detachably connected to the upper end of the ventilation mechanism 13, and a sealing arrangement is formed between the filter element 9 and the ventilation mechanism 13, and the ventilation mechanism 13 includes a connecting disk 1301, and a countersunk through hole is provided on the connecting disk 1301, and an internal thread is provided at the upper end of the countersunk through hole, and the filter element 9 is threadedly installed on the upper end of the countersunk through hole, and a sealing ring 1303 is provided in the countersunk through hole, and the lower end of the filter element 9 is tightly fitted with the sealing ring 1303, and a ventilation fan 1302 is embedded in the lower end of the countersunk through hole;

[0038] The filter element 9 includes a tube body 901, an annular flange 902 is arranged inside the tube body 901, a filter core 903 is arranged at the upper end of the annular flange 902, a magnet block 904 is fixedly installed at the lower end of the filter core 903, the annular flange 902 is arranged as an iron annular flange, and the magnet block 904 is attracted to the annular flange 902;

[0039] The design of the magnet block 904 makes the filter element 903 easy to disassemble, thereby facilitating replacement and maintenance.

[0040] When in use, the water pump 12 is turned on; the first heat exchange water pipe 8 exchanges heat with water in the temperature-adjusting water tank, and then the temperature-adjusted water in the first heat exchange water pipe 8 is connected to the second heat exchange water pipe 10. The second heat exchange water pipe 10 is laid in a curved manner in the incubator, so that the second heat exchange water pipe 10 exchanges heat with the internal space of the incubator, thereby achieving smooth temperature adjustment of the cell culture device, making the temperature change of the cell culture device extremely small, and making it easier to achieve a long-term stable constant temperature state;

[0041] The temperature monitoring sensor 14 monitors the water cooling in the temperature regulating water tank 3. When the water temperature is abnormal, the heating component 5 or the refrigeration component 6 is turned on by the temperature controller 15 to heat the water, and the water is mixed by the stirring mechanism 7 to achieve rapid averaging of the water temperature.

[0042] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell constant temperature culture device, comprising an incubator (1), characterized in that: A mounting box (4) is fixedly connected to one side of the incubator (1), a temperature-controlled water tank (3) is fixedly connected to one side of the mounting box (4), a heating component (5) and a refrigeration component (6) are arranged on the temperature-controlled water tank (3), and a stirring mechanism (7) is arranged inside the temperature-controlled water tank (3); A first heat exchange pipe (8) is arranged in the temperature regulating water tank (3), the first heat exchange pipe (8) being arranged in a spiral shape, a water pump (12) is arranged in the installation box (4), one end of the first heat exchange pipe (8) is connected to a water inlet of the water pump (12), a second heat exchange pipe (10) is connected to a water outlet of the water pump (12), the second heat exchange pipe (10) is plugged into the incubator (1), and the second heat exchange pipe (10) is arranged on the side wall of the incubator (1) in a continuously bent manner, one end of the second heat exchange pipe (10) is connected to one end of the first heat exchange pipe (8), an inner liner (11) is arranged in the incubator (1), and the second heat exchange pipe (10) is located between the inner wall of the incubator (1) and the inner liner (11); A temperature monitoring sensor (14) is fixedly installed in the temperature-adjusting water tank (3), and a temperature controller (15) is fixedly installed on the outer wall of the temperature-adjusting water tank (3); the temperature controller (15) is electrically connected to the temperature monitoring sensor (14), the heating component (5), the refrigeration component (6) and the stirring mechanism (7); Two groups of partitions (16) are arranged in the temperature-adjusting water tank (3), and the two groups of partitions (16) are arranged in an L shape. The temperature-adjusting water tank (3) is divided into a heating chamber, a cooling chamber and a mixing chamber by the two groups of partitions (16). The heating component (5) is located in the heating chamber, the cooling component (6) is located in the cooling chamber, and the stirring mechanism (7) is located in the mixing chamber. The partitions (16) are provided with water-permeable holes. The heating assembly (5) comprises an electric heating plate, a first heat conducting plate (17) is provided at the lower end of the electric heating plate, and the first heat conducting plate (17) extends into the heating cavity; The refrigeration component (6) comprises a semiconductor refrigeration plate (601), the cold end of the semiconductor refrigeration plate (601) is fixedly connected to a second heat conducting plate (18), and one end of the second heat conducting plate (18) extends into the refrigeration cavity; the refrigeration component (6) also comprises a radiator (602), the radiator (602) is fixedly connected to the hot end of the semiconductor refrigeration plate (601), the radiator (602) is configured as a tower radiator, and fans (603) are fixedly installed at both ends of the radiator (602); The stirring mechanism (7) comprises a stirring rod (701), the stirring rod (701) being provided with a stirring blade, the stirring rod (701) being located between two groups of partitions (16), the water-permeable holes on the two groups of partitions (16) being opened on opposite side walls of the two groups of partitions (16), and the lower end of the stirring rod (701) extending to be close to the first water exchange pipe (8); The stirring mechanism (7) further comprises a motor (702), wherein the motor (702) is fixedly mounted on the upper end of the temperature-controlled water tank (3), one end of the output shaft of the motor (702) is inserted into the temperature-controlled water tank (3), and one end of the output shaft of the motor (702) is fixedly connected to the upper end of the stirring rod (701); A ventilation box (2) is fixedly mounted on the upper end of the installation box (4), one end of the ventilation box (2) is plugged into the inner liner (11), and the ventilation box (2) is connected to the inner liner (11), and an air inlet is opened at the upper end of the ventilation box (2); The second hot water exchange pipe (10) passes through the ventilation box (2), and the portion of the second hot water exchange pipe (10) located inside the ventilation box (2) is arranged in a circuitous manner; A ventilation mechanism (13) is embedded and fixed in the air inlet, and a filter element (9) is detachably connected to the upper end of the ventilation mechanism (13), and a sealing arrangement is formed between the filter element (9) and the ventilation mechanism (13); The ventilation mechanism (13) comprises a connection plate (1301), a countersunk through hole is formed on the connection plate (1301), an internal thread is formed at the upper end of the countersunk through hole, the filter element (9) is threadedly mounted on the upper end of the countersunk through hole, a sealing ring (1303) is provided in the countersunk through hole, the lower end of the filter element (9) is tightly fitted with the sealing ring (1303), and a ventilation fan (1302) is embedded at the lower end of the countersunk through hole.

2. A cell constant temperature culture device according to claim 1, characterized in that: The filter element (9) comprises a tube body (901), an annular flange (902) is arranged inside the tube body (901), a filter core (903) is arranged at the upper end of the annular flange (902), a magnet block (904) is fixedly mounted at the lower end of the filter core (903), the annular flange (902) is arranged as an iron annular flange, and the magnet block (904) is attracted to the annular flange (902).

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