Dry fluorescence immunoassay analyzer based on multi-channel and automated detection

Abstract

This invention discloses a dry fluorescence immunoassay analyzer for multi-channel and automated detection. The analyzer rack includes a sample module, a mixing module, a pipette tip module, a cap removal module, a sample application module, an incubation module, a transport module, and a reagent cartridge module. The transport module is connected to both the reagent cartridge module and the incubation module. The sample application module is located above the transport module's movement path and is connected to the pipette tip module. The cap removal module is connected to the mixing module, and the sample module is connected to both the cap removal module and the mixing module. The analyzer utilizes a combination of traditional dry chemistry, immunochromatography, optical information, and data analysis techniques. By employing biomedical and chromatographic technologies to analyze and process chromatographic, spectral, and biosensor information, it applies these techniques to immunoassay and rapid detection, achieving multi-channel and automated detection, improving its on-site applicability, and exhibiting advantages of automation and simplicity.

Description

Technical Field

[0001] This invention relates to the field of immunoassay analyzer technology, specifically a dry fluorescence immunoassay analyzer based on multi-channel and automated detection. Background Technology

[0002] In recent years, due to environmental and other factors, the emergence and sudden occurrence of infectious diseases have continuously threatened human health. The spread of infectious diseases such as SARS, MERS, influenza, Ebola, Zika virus, yellow fever, and Rift Valley fever has become a global public health issue. With urbanization and increased global trade, environmental changes have exacerbated the risk of transmission of zoonotic infectious diseases, making the prevention and control of infectious diseases an urgent priority. Therefore, the development of rapid and timely detection of infectious diseases is of great significance for the prevention and control of infectious diseases.

[0003] At the same time, rapid testing of human blood samples can effectively and quickly detect diseases that have already occurred or are potential diseases in the human body, which is of great significance for effective treatment and early prevention of diseases.

[0004] Early immunoassay products were qualitative, with immunoassay projects serving as research subjects, and colloidal gold was used. Colloidal gold products were the star products of this stage. Based on the principle of antigen-antibody binding, theoretically, almost all protein molecules could be labeled. However, their drawbacks included low sensitivity and accuracy, only able to distinguish between negative and positive results. Later, with technological advancements, colored microsphere labeling was adopted. Compared to colloidal gold, colored microsphere labeling offered improved sensitivity and strong repeatability, but its sensitivity remained limited. With further technological development, fluorescent microsphere labeling technology was applied. Fluorescent microsphere labeling provides strong and stable luminescence, high biocompatibility, and is largely unaffected by changes in the external environment, enabling quantitative detection. However, it places higher demands on the product manufacturing process, increasing the complexity of the assay.

[0005] Point-of-care testing (POCT) products offer advantages such as ease of operation, portability, and intelligent features. In recent years, they have developed rapidly as an in vitro diagnostic technology and are being applied in testing across multiple disciplines. Their rapid result acquisition benefits patients, reducing the time required for diagnosis. Furthermore, POCT products can be used for continuous monitoring of the condition; for example, heart disease patients can quickly understand and manage their condition through interval testing.

[0006] Existing equipment requires a large number of tedious manual steps, making operation cumbersome and unable to achieve automation, while also having a single detection channel. Summary of the Invention

[0007] The purpose of this invention is to overcome the problems mentioned in the background art and provide a dry fluorescence immunoassay analyzer based on multi-channel and automated detection. This analyzer uses biomedical technology and chromatography technology to analyze and process chromatographic, spectral, and biosensor information, and applies it to immunoassay and rapid detection, realizing multi-channel and automated detection, improving its on-site applicability, and exhibiting the advantages of automation and simplicity.

[0008] The objective of this invention is mainly achieved through the following technical solutions:

[0009] A dry fluorescence immunoassay analyzer for multi-channel and automated detection includes a frame on which a sample module, a mixing module, a pipette tip module, a cap removal module, a sample application module, an incubation module, a transport module, and a reagent cartridge module are mounted. The transport module is connected to both the reagent cartridge module and the incubation module. The sample application module is located above the movement trajectory of the transport module and is connected to the pipette tip module. The cap removal module is connected to the mixing module. The sample module is connected to both the cap removal module and the mixing module.

[0010] Fluorescence immunoassay, also known as fluorescent antibody technique, is one of the earliest developed labeled immunoassay techniques. It is a technology built upon immunology, biochemistry, and microscopy. In a dry immunofluorescence analyzer, the sample solution dropped onto one end of a membrane is propelled to the other end by the membrane's capillary action (lateral flow based on chromatography). During this movement, the analyte binds to receptors (antigens or antibodies) immobilized in a specific region of the membrane and becomes immobilized. Irrelevant substances cross this region and are separated. The test results are then determined by label detection (qualitative, semi-quantitative, and quantitative). Fluorescence immunoassay analyzers are primarily suitable for blood biochemical analysis and testing in various medical institutions, such as the testing of hormones and infectious diseases.

[0011] Existing fluorescence immunoassay analyzers require numerous cumbersome manual steps for auxiliary detection. To address this issue, this solution integrates a sample module, mixing module, pipette tip module, cap removal module, sample loading module, incubation module, transport module, and reagent cartridge module on the rack. These modules are arranged and connected as needed to meet operational requirements. Utilizing biomedical and chromatographic techniques, the solution analyzes and processes chromatographic, spectral, and biosensor information for immunoassay and rapid detection, achieving multi-channel and automated detection, improving its on-site applicability, and demonstrating the advantages of automation and simplicity. The use of fluorescent microsphere labeling combined with optical information technology makes rapid detection and reaction more controllable, significantly improving detection sensitivity and precision, representing one of the future technological development directions.

[0012] Furthermore, the sample module includes a sample rack base plate, which is fixed to the frame. A sample rack is mounted on the sample rack base plate, located below and movably connected to the cap removal module and the shaking module. A first drive mechanism is located below the sample rack base plate, connected to an injection fork. The injection fork is connected to the sample rack, and under the action of the first drive mechanism, the sample rack and injection fork can move horizontally relative to the sample rack base plate. A transverse base plate is located outside the sample rack base plate, on which a second drive mechanism and a scanner are mounted. After the sample rack moves under the action of the first drive mechanism, it can connect to the second drive mechanism, and under the action of the second drive mechanism, it moves horizontally relative to the sample rack base plate and passes through the scanner station. A third drive mechanism is located below the sample rack base plate, connected to an ejection fork. The ejection fork connects after the sample rack moves with the second drive mechanism, and under the action of the third drive mechanism, the sample rack can move horizontally relative to the sample rack base plate. This solution, by dividing the injection and ejection processes into zones, achieves a simple cycle to increase the number of samples that can be placed at one time, thus improving the efficiency of the supporting instruments. It can automatically move the sample rack and automatically read sample information.

[0013] Furthermore, the shaking module includes a support mechanism, which is fixed to the frame. A shaking body and a fourth driving mechanism are mounted on the support mechanism. The fourth driving mechanism is fixed to the support mechanism. The shaking body and the fourth driving mechanism are simultaneously connected to the support mechanism, and the shaking body can rotate around the connection point of the support mechanism under the drive of the fourth driving mechanism. The sample holder and the cap removal module are movably connected to the shaking body. A clamping mechanism and a fifth driving mechanism are provided below the shaking body, and the clamping mechanism can move relative to the support mechanism under the drive of the fifth driving mechanism to achieve clamping. The clamping mechanism, which can be either released or adjusted, includes a shaking clamp, a pressure block, and a linear guide rail. The shaking clamp and the linear guide rail are connected, and the linear guide rail is fixed to the support mechanism. The pressure block and the shaking clamp are fixed, and the pressure block and the shaking body are elastically connected. The shaking clamp can move along the linear guide rail. The shaking clamp is connected to a drive mechanism, which is connected to a clamping mounting plate. Rollers are provided on the clamping mounting plate. Under the action of the drive mechanism, the clamping mounting plate moves linearly, causing the rollers to enter between the pressure block and the shaking clamp and contact the shaking clamp. Currently, most in vitro diagnostic analyzers still rely on manual mixing of sample tubes, resulting in a large workload. Alternatively, a separate device is used for mixing, which requires manual transfer into the instrument for testing, increasing workload and waiting time, failing to meet the current demand for efficient and automated operation. Furthermore, if a separate device is used for mixing sample tubes in existing in vitro diagnostic analyzers, it would increase equipment costs. To address the aforementioned issues, this solution employs the structure described above. The fourth driving mechanism rotates the shaking body, which can accommodate multiple test tubes as needed. The fifth driving mechanism moves the clamping mechanism to clamp or release the sample test tubes. This allows for simultaneous clamping and shaking of multiple test tubes with a compact structure. It automatically clamps and releases the test tubes and can simulate manual operation to achieve 90-degree rotation and mixing of the sample. The entire process is automated, precise, and efficient.

[0014] Furthermore, the cap-removing module includes a horizontal plate frame, which is fixed to the frame. A moving mechanism and several grippers are mounted on the horizontal plate frame. The grippers are all connected to the moving mechanism and can move relative to the horizontal plate frame under its influence. The grippers are movably connected to the shaking body and the sample holder. A clamping mechanism is provided on the horizontal plate frame, which is connected to the grippers and clamps or loosens under its action. A rotating mechanism is provided on the horizontal plate frame, which is connected to the grippers and allows the grippers to rotate around their own axis under its action. Currently, most instruments rely on manual extraction of reagent samples. This manual operation is cumbersome, prone to secondary contamination, and the single-use extraction and testing of sample tubes results in low efficiency, high labor intensity, and the risk of abnormal results requiring the provision of new reagent samples. Some manufacturers have also begun to try using automatic cap removal function. Currently, in vitro diagnostic analyzers with automatic cap removal function on the market have limited functions and complex structures. Furthermore, reagent samples cannot be extracted and tested multiple times and can only be extracted and tested once. Therefore, it is essential to have a device that can perform multiple tests on different items for multiple people at the same time and can perform multiple cap removal and capping of reagent samples for preservation. This solution is a compact device that can simultaneously rotate and cap multiple test tubes. It can automatically detect the presence or absence of test tube caps and complete the cap removal and capping process. The entire process is automated, precise, and efficient. The device is designed so that the grippers are connected to the moving mechanism and can move relative to the horizontal frame under the action of the moving mechanism. The clamping mechanism is connected to the grippers and can clamp or loosen them under the action of the clamping mechanism. The rotating mechanism is connected to the grippers and can rotate the grippers around their own axis under the action of the rotating mechanism. Through the above structure, it can simultaneously rotate and cap multiple test tubes, and can also automatically detect the presence or absence of test tube caps and complete the cap removal and capping process with precision and efficiency.

[0015] Furthermore, the pipette tip module includes a pipette tip box mounting plate, which is fixed to the frame. A pipette tip box is mounted on the mounting plate, and a drive mechanism six is ​​also mounted on the mounting plate. The drive mechanism six is ​​connected to the pipette tip box, and under the action of the drive mechanism six, the pipette tip box can move relative to the mounting plate. During the movement of the pipette tip box, it connects to the sample dispensing module. A drive mechanism seven is also mounted on the mounting plate, and a puncture head is connected to the drive mechanism seven. The puncture head moves vertically under the action of the drive mechanism seven. This structure enables automated movement of the pipette tip to the corresponding position, and the puncture head punctures the membrane covering the reagent strip. The entire process is automated, reducing manual operation.

[0016] Furthermore, the sample loading module includes a sample loading column, which is fixed to the frame. A first sample loading horizontal plate is installed between the sample loading columns, and the first sample loading horizontal plate is fixed to the sample loading column. A second sample loading horizontal plate is installed between the first sample loading horizontal plate, and the second sample loading horizontal plate can move along the first sample loading horizontal plate. A nozzle is installed on the second sample loading horizontal plate, and the nozzle can move along the second sample loading horizontal plate. At the same time, the nozzle can move vertically and be inserted into the pipette tip box, the mixing body, or the sample holder. Since the entire sample loading process involves multiple points, multi-directional movement is required. Existing chemiluminescence immunoassay analyzers have complex movement during the sample loading process and are difficult to move to the correct position, making it impossible to complete subsequent functions such as automatic pipette tip addition and removal, sample loading, and sample sorting. This solution uses the horizontal plate for horizontal movement and the nozzle for horizontal and vertical movement, enabling it to move to various preset positions within the structure, thus realizing functions such as automatic pipette tip addition and removal, sample loading, and sample sorting.

[0017] Furthermore, the incubation module includes an incubation data acquisition mechanism and a waste strip rejection mechanism. The transport module is movable relative to the incubation data acquisition mechanism and the waste strip rejection mechanism, and the transport module is movably connected to these two mechanisms. The incubation data acquisition mechanism is equipped with a heating plate and a temperature sensor, with the temperature sensor connected to the heating plate. Currently, chemiluminescence immunoassay analyzers do not consider the incubation process separately; the incubation component and transport component are fixed together. This structure results in fewer incubation compartments, affecting instrument testing efficiency. Additionally, the addition and removal of reagent strips are all done manually, making the entire process cumbersome and inefficient. This solution, however, sets up an incubation data acquisition mechanism and a waste strip rejection mechanism, combined with a transport module, allowing the three components to operate independently without interference. They work together as needed for the process, achieving independent incubation component settings, thereby increasing the number of incubation compartments and improving instrument testing efficiency. Furthermore, the entire process of adding and removing reagent strips is automated, enabling automatic acquisition of reagent luminescence data and automatic rejection of discarded strips. The incubation partition automatically fixes and positions the strips, simplifying the operation and improving work efficiency. It also enables heating and temperature control, resulting in more accurate incubation results.

[0018] Furthermore, the reagent cartridge module includes a cartridge holder with a scanning device mounted on it, and the scanning device is movable relative to the cartridge holder. A hook-and-loop mechanism is externally located on the cartridge holder, and this mechanism is movable relative to the cartridge holder and can be inserted into it. This reagent cartridge module can simultaneously hold multiple reagent cartridges for different items, and one cartridge can hold multiple single-use reagent strips. It also features a cartridge placement detection system to monitor the real-time status of the cartridges, and the scanner can automatically read the cartridge information. The hook-and-loop mechanism further enables automatic removal of reagent strips, increasing the number of reagent strips that can be placed at once and improving the efficiency of the accompanying instruments.

[0019] Furthermore, the transport mechanism includes a transport plate 1, which is connected to a drive mechanism 8 and can move horizontally under the action of the drive mechanism 8. A drive mechanism 17 and a transport plate 2 are provided on the transport plate 1, and the drive mechanism 17 and the transport plate 2 are connected. The transport plate 2 moves horizontally relative to the transport plate 1 under the action of the drive mechanism 17. A transport fork and a drive mechanism 9 are provided on the transport plate 2. The drive mechanism 9 is installed on the transport plate 2, and the transport fork is connected to the drive mechanism 9. The transport fork can move vertically under the action of the drive mechanism 9 and is connected to the hook mechanism.

[0020] In summary, the present invention has the following advantages compared with the prior art:

[0021] (1) This invention can realize automatic sample entry and exit and read sample information;

[0022] (2) The present invention can realize automatic handling, cap removal and cap closing of sample tubes;

[0023] (3) This invention can achieve automatic sample mixing;

[0024] (4) This invention can realize automatic addition, removal and status detection of suction heads;

[0025] (5) This invention can achieve simultaneous multi-sample testing;

[0026] (6) This invention can achieve automatic sample dilution and mixing;

[0027] (7) This invention enables automatic pickup and transfer of reagent strips;

[0028] (8) This invention enables mobile and dynamic light collection;

[0029] (9) This invention can realize the detection of the liquid level in the sampled liquid;

[0030] (10) The present invention adopts a modular design, making the integration of the whole machine faster and more flexible. Attached Figure Description

[0031] The accompanying drawings, which are included to provide a further understanding of embodiments of the invention and form part of this application, do not constitute a limitation thereof. In the drawings:

[0032] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0033] Figure 2 This is a schematic diagram of the sample module.

[0034] Figure 3 This is a schematic diagram of the shaking module.

[0035] Figure 4 This is a schematic diagram of the suction head module.

[0036] Figure 5 This is a schematic diagram of the cap removal module.

[0037] Figure 6 This is a schematic diagram of the sample addition module.

[0038] Figure 7 This is a schematic diagram of the incubation module.

[0039] Figure 8 This is a schematic diagram of the control module.

[0040] Figure 9 This is a structural diagram of the transportation module.

[0041] Figure 10 This is a schematic diagram of the reagent cartridge module.

[0042] Figure 11 This is a schematic diagram of the reagent cartridge module from another direction.

[0043] The names corresponding to the reference numerals in the attached figures are:

[0044] 1-Sample module, 2-Shaking module, 3-Pipette tip module, 4-Cap removal module, 5-Sample addition module, 6-Control module, 7-Incubation module, 8-Transportation module, 9-Reagent cartridge module, 10-Drive mechanism one, 11-Sensor one, 12-Synchronous pulley and synchronous belt one, 13-Scanner, 14-Emergency station, 15-Drive mechanism two, 16-Drive mechanism one, 17-Sample rack limiting plate, 18-Mounting plate one, 19-Sample injection fork, 20-Synchronous pulley and synchronous belt two, 21-Optical coupler one, 22-Column one, 23-Guide rail three, 24-Synchronous pulley and synchronous belt three, 25-Drive mechanism four, 26-Shaking mounting plate, 27-Shaking body, 28-Shaking column, 29-Roller, 30-Clamping mounting plate 31-Drive Mechanism Five, 32-Limit Block, 33-Headpiece Box Mounting Plate, 34-Linear Guide Rail Eight, 35-Rack, 36-Drive Mechanism Six, 37-Limit Plate, 38-Drive Mechanism Seven, 39-Linear Guide Rail Nine, 40-Four-Edge Knife Mounting Plate, 41-Four-Edge Knife, 42-Column Two, 43-Headpiece Box Placement Plate, 44-Headpiece Box Set, 45-Gripper, 46-Moving Bracket, 47-Clamping Bracket, 48-Drive Mechanism Eleven, 49-Drive Mechanism Ten, 50-Horizontal Plate Connector, 51-Linear Guide Rail Seven, 52-Synchronous Belt Pulley and Synchronous Belt Four, 53-Drive Mechanism Twelve, 54-Sensor Two, 55-Sample Loading Horizontal Plate One, 56-Archer Mounting Plate, 57-Drive Mechanism Twelve, 58-Guide Rail Ten, 59-Archer, 6 0-Synchronous pulley and synchronous belt five, 61-Guide rail eleven, 62-Drive mechanism thirteen, 63-Cable protection cable chain one, 64-Sample loading column, 65-Scrap card baffle, 66-Suction head removal bracket, 67-Synchronous pulley and synchronous belt six, 68-Drive mechanism sixteen, 69-Drive mechanism fifteen, 70-Cable protection cable chain two, 71-Guide rail twelve, 72-Incubation ruler, 73-Synchronous pulley and synchronous belt seven, 74-Optical coupler two, 75-Drive mechanism fourteen, 76-Incubation column, 77-Incubation base plate, 78-Optical module, 79-Push card base plate, 80-Push card fork, 81-Main control board two, 82-Mounting plate three, 83-Drive board one, 84-Drive board two, 85-Drive board three, 86-Switching power supply, 87-Same 88-Cable protection cable chain 3, 89-Sensor 3, 90-Drive mechanism 17, 91-Drive mechanism 8, 92-Optical coupler 3, 93-Guide rail 14, 94-Drive motor 19, 95-Transport fork, 96-Code teeth, 97-Guide rail 15, 98-Reagent card holder column, 99-Reagent card holder base plate, 100-Reagent card holder, 101-Drive mechanism 19, 102-Guide rail 17, 103-Hook and code teeth, 104-Cable protection cable chain 4, 105-Drive mechanism 21, 106-Drive mechanism 20, 107-Hook and base plate, 108-Synchronous pulley and synchronous belt 9, 109-Rear plate, 110-Synchronous pulley and synchronous belt 10, 111-Drive mechanism 18, 112-Detection plate.113-Cover plate, 114-Side plate, 115-Scanning device, 116-Guide rail sixteen, 117-Hook and clip horizontal plate, 118-Synchronous pulley and synchronous belt eleven, 119-Hook and clip fork, 120-Sample tube. Detailed Implementation

[0045] To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments and accompanying drawings. The illustrative embodiments and descriptions of the present invention are only used to explain the present invention and are not intended to limit the present invention.

[0046] Example 1:

[0047] like Figure 1 As shown, this embodiment includes a frame on which are mounted a sample module 1, a shaking module 2, a pipette tip module 3, a cap removal module 4, a sample application module 5, a control module 6, an incubation module 7, a transport module 8, and a reagent cartridge module 9. The transport module 8 is connected to both the reagent cartridge module 9 and the incubation module 7. The sample application module 5 is located above the movement trajectory of the transport module 8 and is connected to the pipette tip module 3. The cap removal module 4 is connected to the shaking module 2. The sample module 1 is connected to both the cap removal module 4 and the shaking module 2. The sample module 1, shaking module 2, pipette tip module 3, cap removal module 4, sample application module 5, incubation module 7, transport module 8, and reagent cartridge module 9 are all connected to the control module 6. By arranging and connecting these components as needed, the system meets the requirements for use. It utilizes biomedical and chromatographic technologies to analyze and process chromatographic, spectral, and biosensor information, applying it to immunoassay and rapid detection. This enables multi-channel and automated detection, improving its on-site applicability and demonstrating the advantages of automation and simplicity.

[0048] Example 2:

[0049] like Figure 2As shown, the sample module 1 in this embodiment includes a sample rack base plate. A mounting plate 18 is positioned below the sample rack base plate. The mounting plate 18 and the sample rack base plate are supported by a column 22, forming sufficient mounting space at the bottom. The sample rack base plate is fixed to the frame. A sample rack is mounted on the sample rack base plate, located below and movably connected to the cap removal module and the shaking module. The sample racks are named the test sample rack, the experimental sample rack, and the measured sample rack according to their different processes. A drive mechanism 16, a synchronous pulley, and a synchronous belt 20 are positioned below the sample rack base plate. The synchronous belt is connected to an injection fork 19, which is located in a corresponding slide rail on the sample rack base plate. The injection fork 19 can move along the slide rail. The injection fork 19 is connected to a guide rail 1, and the injection fork 19 can move along the guide rail 1. An optocoupler 21 is provided at the extreme positions of the injection fork 19 to limit its movement distance. The synchronous belt is moved by the drive mechanism 16, which causes the sample fork 19 to move in the slide, thereby pushing the sample holder to be tested to move horizontally on the sample holder base plate.

[0050] A transverse base plate is provided on the outside of the sample rack base plate. A second drive mechanism 15, a synchronous pulley, a first synchronous belt 12, and a scanner 13 are mounted on the transverse base plate. A transverse shift fork is connected to the synchronous belt. When the sample rack to be tested moves to its limit position under the action of the first drive mechanism 16, the transverse shift fork on the synchronous belt connected to the second drive mechanism 15 engages with the sample rack, making it a test sample rack. A second guide rail is mounted on the transverse base plate. After the sample rack is connected to the second drive mechanism 15, it is also connected to the second guide rail and can move along the second guide rail. The second drive mechanism 15 drives the test sample rack to move horizontally relative to the sample rack base plate and passes through the scanner station for barcode information reading.

[0051] A drive mechanism 10, a synchronous pulley, and a synchronous belt 24 are located below the sample holder base plate. The drive mechanism 10 is connected to a sample ejection fork, which is positioned in a corresponding slide rail on the sample holder base plate and can move along the slide rail. The sample ejection fork is connected to a guide rail 23 and can move along the guide rail 23. After scanning, when the sample holder moves to a preset position, the sample ejection fork hooks onto the test sample holder. Under the action of the drive mechanism 10, the sample holder can move horizontally relative to the sample holder base plate, simultaneously disengaging from the synchronous belt in the drive mechanism 15. As the test sample holder moves, it becomes a tested sample holder. A sample holder limit plate 17 is provided on the sample holder base plate as the extreme position and guide for movement. A sensor 11 is also provided on the sample holder base plate for movement position detection. An emergency position 14 is also reserved on the sample holder base plate to further enhance the application scenarios of the complete machine.

[0052] By setting the movement direction of the sample holder under the action of drive mechanism three 16 to be parallel to the movement direction of the sample holder under the action of drive mechanism one 10, and setting the movement direction of the sample holder under the action of drive mechanism two 15 to be perpendicular to the movement direction of the sample holder under the action of drive mechanism three 16, the cycle of the sample holder is finally achieved.

[0053] Working principle:

[0054] The sample to be tested is placed in the sample rack on the right side of the sample rack base plate. When placing the sample rack, ensure the bottom notch is aligned with the sample rack limiting block 17 (foolproof function). Drive mechanism three 16, along with the synchronous pulley, synchronous belt two 20, and sample infeed fork, moves the sample rack to the preset position, and sensor one 11 detects its status. Drive mechanism two 15, along with the synchronous pulley and synchronous belt one 12, moves the sample rack to the preset position, where scanner 13 reads the barcode information. After sample processing, drive mechanism one 10, along with the synchronous pulley, synchronous belt three 24, and sample discharge fork, moves the sample rack to the preset position, and sensor one 11 detects its status. An emergency position 14 is provided in the sample device to enhance the application environment of the supporting instruments. This solution can achieve automatic sample rack movement; automatic sample information reading; sample rack positioning detection; independent placement of emergency samples; simultaneous placement of multiple sample racks; and sample rack placement direction limitation.

[0055] Example 3:

[0056] like Figure 3 As shown, the shaking module 2 includes a support mechanism, which mainly consists of a shaking mounting plate 26 and shaking columns 28. Two shaking columns 28 are vertically arranged, and the shaking mounting plate 26 is fixed to both shaking columns 28. A shaking body 27 and a driving mechanism 25 are provided on the support mechanism, with the driving mechanism 25 using a stepper motor as the shaking drive mechanism. The shaking body 27 has through holes for mounting sample tubes. A shaking cover plate is installed on the top surface of the shaking body 25, exposing the through holes. Sample rack adapters are provided on the shaking cover plate, with each adapter located at the opening of a through hole. The sample tubes in the through holes are fixed by the shaking cover plate and the sample rack adapters. The shaking process does not require clamping the tubes; initial fitting and fixing are achieved through the sample rack adapters. The number of through holes depends on the specific number of sample tubes; in this embodiment, five through holes are preferred. The multi-hole design of the shaking body 27 in this scheme allows for the separate loading and mixing of reagents from multiple samples, improving work efficiency.

[0057] The drive mechanism 25 is fixed by a shaking motor bracket and a shaking mounting plate 26 or a shaking column 28. A deep groove ball bearing is installed in the shaking motor bracket, and a shaking motor bushing is installed between the shaking motor bracket and the shaking body 27. The shaking motor bushing is connected to the shaking body 27. The drive mechanism 25 is mounted on the shaking motor bracket, and its output end passes through the deep groove ball bearing and is connected to the shaking motor bushing, thus transmitting the rotation of the drive mechanism 25 to the shaking body 27 in a timely manner. The other end of the shaking body 27 is connected to the shaking body 27 via an oil-free bushing and a rotating shaft, thus satisfying both the rotation of the shaking body 27 and providing support. With this structural design, the rotation of the drive mechanism 25 can drive the shaking body 27 to rotate around its own axis. The maximum rotation angle is 90°, which, when the sample tube is horizontal, achieves proper shaking. A shaking reset optocoupler is provided on the side wall of the shaking mounting plate 26. When the shaking body 27 rotates to 90°, the shaking body 27 just contacts the shaking reset optocoupler, and the drive mechanism receives the signal and reverses.

[0058] A clamping mechanism and a drive mechanism 31 are provided below the shaking body 27. The drive mechanism 31 preferably uses a lead screw motor as the clamping motor, which facilitates the compactness of the overall structure and the realization of its functions. The clamping mechanism includes a shaking clamp, a pressure block, and a linear guide rail. The pressure block and the bottom of the shaking clamp are fixed, and the shaking clamp and the linear guide rail are connected. The shaking clamp can move along the linear guide rail, which is fixed to the shaking body 27. A compression spring is provided between the pressure block and the shaking clamp, and the compression spring is connected to both the pressure block and the shaking clamp. During operation, the bottom of the sample tube is located between the pressure block and the shaking clamp. A roller 29 is provided on the clamping mounting plate 30. Under the action of the drive mechanism 31, the clamping mounting plate 30 moves linearly, causing the roller 29 to enter between the pressure block and the shaking clamp and contact the shaking clamp. Multiple sets of rollers 29 are used. To facilitate the insertion of the rollers 29 between the pressure block and the shaking clamp, the contact surfaces between the shaking clamp and the rollers 29 are designed as bevels. The relatively close surfaces of the pressure block and the shaking clamp form an opening, making it easier for the rollers to squeeze in and enabling rapid release. Simultaneous force application at multiple points allows for simultaneous action, ensuring that the release of all sample tubes is synchronized.

[0059] The drive mechanism 5 31 and the clamping mounting plate 30 are connected by a lead screw and nut pair, which converts the rotation of the drive mechanism 5 into the linear movement of the clamping mounting plate 30. A limit guide rail is provided on the clamping mounting plate 26, and a sliding sleeve is fixed on the clamping mounting plate 30. The sliding sleeve is fitted on the limit guide rail, and the clamping mounting plate 30 can move along the limit guide rail, thereby limiting the movement trajectory.

[0060] A clamping reset optocoupler is also provided on the mounting base of the drive mechanism 31, located below the clamping mounting plate 30. The clamping reset optocoupler limits the moving distance of the moving clamping mounting plate 30. When it moves to the bottom and contacts the clamping reset optocoupler, the drive mechanism 31 stops rotating or rotates in the opposite direction. The clamping mounting plate 30 is also provided with a limit block 32 to limit the position.

[0061] Five sample tubes are simultaneously fixed inside the shaking body 27 via the sample rack adapter and the shaking cover. The shaking process is as follows: the shaking stepper motor mounted on the shaking motor bracket transmits rotational power to the shaking body 27 through the connection of a deep groove ball bearing, an oil-free bushing, a rotating shaft, and a shaking motor bushing. Rotating the shaking body 27 achieves a 90-degree rotation of the sample tubes, thereby stirring and mixing the reagents inside the sample tubes. The clamping and releasing process is as follows: the clamping screw motor drives the clamping mounting plate 30 to move upward linearly, causing the roller 29 to enter between the shaking clamp and the pressure block. The pressure block remains stationary, and the shaking clamp, under pressure, moves along the linear guide rail five, reducing the gap between the shaking clamp and the pressure block, thus clamping the sample tube. The clamping screw motor rotates in the opposite direction, driving the clamping mounting plate 30 downward linearly. The roller 29 gradually retracts from between the shaking clamp and the pressure block. Through the elastic force of the compression spring, the shaking clamp and the pressure block are gradually released. Under the action of the compression spring, the shaking clamp moves along the linear guide rail five, finally releasing the sample tube. This invention integrates the shaking and clamping / releasing processes, resulting in a compact structure and reliable operation. It can simultaneously simulate manual shaking and testing of multiple sets of sample tubes, meeting the needs of multi-sample testing while achieving efficient operation and automation.

[0062] Example 4:

[0063] like Figure 4As shown, the suction tip module 3 includes a suction tip box mounting plate 33, which is fixed to the frame by a column 42. A suction tip box placement plate 43 is provided on the suction tip box mounting plate 33. The suction tip box set 44 is integrally located on the suction tip box placement plate 43. A drive mechanism 36 is provided on the suction tip box mounting plate 33. A gear is fitted on the output end of the drive mechanism 36. The gear meshes with a rack 35. The rack 35 is connected to the suction tip box placement plate 43. Under the action of the drive mechanism 36, the rack 35 moves and pulls the suction tip box set 44 on the suction tip box placement plate 43 to move. During the movement of the suction tip box set 44, it connects with the sample dispensing module. A linear guide rail 34 is also provided on the suction head box mounting plate 33. The suction head box placement plate 43 is connected to the linear guide rail 34 and can move along the linear guide rail 34. A limit plate 37 is installed at the end of the linear guide rail 34. A puncture mounting plate is provided on the suction head box mounting plate 33. A drive mechanism 38 is fixed on the puncture mounting plate. The drive mechanism 38 is connected to a four-edged knife mounting plate 40. The four-edged knife mounting plate 40 and the puncture mounting plate are connected by a linear guide rail 39. A four-edged knife 41 is installed on the four-edged knife mounting plate 40 as a puncture head. Under the action of the drive mechanism 38, the four-edged knife 41 moves vertically. The pipette tip box set 44 is placed on the pipette tip box placement plate 43. The movement of the pipette tip box can be achieved by gears and drive mechanism six 36, rack 35 and linear guide rail eight 34. The four-sided blade 41 is installed on the four-sided blade mounting plate 40. It can be driven by drive mechanism seven 38 and linear guide rail nine 39 to achieve vertical movement and realize the breaking of the film on the reagent card strip.

[0064] Example 5:

[0065] like Figure 5 As shown, the cap-removing module 4 includes a horizontal plate frame 50, which is made of sheet metal and is fixed to the frame. A drive mechanism 49 is mounted on the horizontal plate frame 50 as the power mechanism for the moving mechanism. The drive mechanism 49 is connected to the moving bracket 46. The drive mechanism 49 is preferably a lead screw motor. The drive mechanism 49 and the moving bracket 46 are connected through a lead screw and nut pair structure. The rotation of the drive mechanism 49 drives the moving bracket 46 to move linearly. The moving bracket 46 is also mounted on the horizontal plate frame and is movably connected to it. The moving bracket 46 has an L-shaped structure. A linear guide rail 6 is provided on the horizontal plate frame 6 and is fixed to the horizontal plate frame. The axial direction of the linear guide rail 6 is the same as the moving direction of the moving bracket 46. The moving bracket 46 is connected to the linear guide rail 6 through a slide groove and can move along the axial direction of the linear guide rail 6.

[0066] A clamping mechanism is provided on the movable support 46. The clamping mechanism includes a clamping bracket 47 and a drive mechanism 11 48 connected to each other. The drive mechanism 11 48 also uses a lead screw motor and is connected to the clamping bracket 47 through a lead screw and nut pair structure. The drive mechanism 11 48 drives the clamping bracket 47 to move linearly. The clamping bracket 47 is mounted on the movable support 46 and is movably connected to the movable support 46. A linear guide rail 7 51 is provided on the movable support 46 and is fixed to the movable support 46. The linear guide rail 7 51 and the clamping bracket 47 move in the same direction. The clamping bracket 47 is connected to the linear guide rail 7 51 through a sliding groove and can move along the axis of the linear guide rail 7 51.

[0067] A cap-pulling shaft is installed on the clamping bracket 47, and a deep groove ball bearing is installed on the clamping bracket 47. The top end of the cap-pulling shaft passes through the deep groove ball bearing and is connected to the deep groove ball bearing, thereby fixing the cap-pulling shaft and the clamping bracket 47.

[0068] The rotating mechanism includes a drive mechanism 1253 and a transmission mechanism. The transmission mechanism consists of a synchronous pulley and a synchronous belt 452. The drive mechanism 1253 uses a stepper motor. The drive mechanism 1253, the synchronous pulley, and the synchronous belt 452 are all mounted on the moving bracket 46. The output end of the drive mechanism 1253 is connected to the driving wheel in the transmission mechanism. The driven wheel in the transmission mechanism is connected to a gripper bracket. The gripper bracket is located below the moving bracket 46 and has several grippers 45 mounted on it. The nut-pulling shaft passes through the driven wheel in the transmission mechanism and connects to the corresponding gripper 45. This does not affect the rotation and clamping actions, and they do not interfere with each other. Each gripper 45 is equipped with a gripper pad, and a sensor 54 is installed at the gripper to detect the presence or absence of caps on the sample tubes. This design preferably uses five sets of grippers 45, simultaneously enabling the capping or uncapping of five sets of sample tubes. The grippers 45 have an open structure, with the end of the sample tube located within the opening. The opening size changes by moving the cap-removing rotating shaft. As the clamping bracket 47 moves, the cap-removing rotating shaft drives the grippers 45 to clamp or loosen. The driven wheel is connected to the moving bracket 46 via a deep groove ball bearing, enabling rotational control of the gripper bracket.

[0069] For ease of control, a drive plate is also provided, which is mounted on top of the movable bracket 46 via a horizontal plate. The horizontal plate and the top of the movable bracket 46 are fixed together. The drive plate is connected to a cable chain and is also connected to drive mechanism 10 49, drive mechanism 11 48, and drive mechanism 12 53. Drive mechanism 10 49 and the horizontal plate form a lead screw and nut pair connection, which is more reasonable in structure.

[0070] Existing technologies are inefficient and cumbersome to operate manually. Most in vitro diagnostic analyzers still require manual removal and capping of sample tubes, resulting in a large workload. Alternatively, they may only offer the removal function, preventing multiple extractions and tests. This fails to meet current demands for equipment compatibility, high efficiency, and automation. Furthermore, the level of equipment intelligence is low; existing in vitro diagnostic analyzers generally lack detection for the presence or absence of caps on sample tubes. The presence of uncapped sample tubes can easily lead to equipment malfunctions. This solution integrates the removal and capping of sample tubes into a single unit, allowing simultaneous removal and capping of multiple sets of sample tubes. Its compact structure ensures reliable and efficient operation. It can simultaneously detect the presence or absence of caps on multiple sets of sample tubes, implementing closed-loop control to prevent equipment malfunctions and achieving the requirements for efficient and automated operation. The compact design also reduces intermediate waiting time and improves work efficiency.

[0071] The working principle of this solution is as follows:

[0072] (1) Cap removal process: Drive mechanism 10 49 drives 5 grippers 45 to move simultaneously in the Z direction to the cap removal position of sample tube 120. Then drive mechanism 11 48 is activated, driving clamping bracket 47 and cap removal shaft to move upward in a straight line, so that 5 grippers 45 perform a tightening action together, thereby clamping the caps of 5 sample tubes 120. Then drive mechanism 12 53 works to drive the synchronous belt and gripper bracket to make 5 grippers 45 rotate. At the same time, drive mechanism 10 49 works to drive grippers 45 to move upward, thereby realizing the cap removal action of 5 sample tubes 120.

[0073] (2) Capping process: After the capping is completed above, the five grippers 45 can be moved downward by the operation of the drive mechanism 11 49. At the same time, the drive mechanism 12 53 drives the synchronous belt and the gripper bracket to make the five grippers 45 rotate until the grippers 45 move to the capping position. When the test tube cap enters the sample tube 120, the drive mechanism 11 48 moves synchronously, driving the clamping bracket 47 and the capping shaft to move downward in a straight line together, so that the five grippers 45 open, thus capping the five sample tubes 120.

[0074] Example 6:

[0075] like Figure 6As shown, the sample application module includes sample application columns 64, which are fixed to the frame. The sample application columns 64 have a cuboid structure. Sample application horizontal plates 55 are arranged between the sample application columns 64, and each horizontal plate 55 is fixed to its corresponding column. A drive mechanism 13 62 is installed on the horizontal plate 55. A second horizontal plate serves as a gun mounting plate 56 between the horizontal plates 55. The gun mounting plate 56 is installed on the horizontal plate 55 and connected to the drive mechanism 13 62 via a synchronous pulley and a synchronous belt 5 60. Under the action of the drive mechanism 13 62, it moves along the horizontal plate 55. A cable protection drag chain 63 is connected to the drive mechanism 13 62. A guide rail 10 58 is installed on the horizontal plate 55. The gun mounting plate 56 is connected to the guide rail 10 58 and can move along the guide rail 10 58. A synchronous pulley and a synchronous belt 60 are mounted on the sample loading horizontal plate 55. The driving pulley is connected to the drive mechanism 62. The synchronous belt is mounted on the driving and driven pulleys of the synchronous pulley. There are multiple driven pulleys, which enables the synchronous belt to be connected. A portion of the synchronous belt is located on the sample loading horizontal plate 55. The nozzle mounting plate 56 is connected to the portion of the synchronous belt located on the sample loading horizontal plate 55. The drive mechanism 62 moves the nozzle mounting plate 56 along the guide rail 58 on the sample loading horizontal plate 55 by driving the synchronous belt. A nozzle 59 is provided on the nozzle mounting plate 56. The nozzle 59 can move vertically and be inserted into the pipette tip box, the shaking body, or the sample holder.

[0076] A drive mechanism 12 57 is mounted on the gun mounting plate 56. A synchronous pulley and a synchronous belt are also mounted on the gun mounting plate 56. The driving pulley of the synchronous pulley is connected to the drive mechanism 12 57. The synchronous belt is connected to both the driving and driven pulleys of the synchronous pulley. The sample application mechanism is mounted on the gun mounting plate 56 and connected to the synchronous belt, and moves along the gun mounting plate 56 under the action of the drive mechanism 12 57. A guide rail 11 61 is mounted on the gun mounting plate 56. The gun 59 is connected to the guide rail 11 61 and can move along the guide rail 11 61. Both the drive mechanism 13 62 and the drive mechanism 12 57 are stepper motors.

[0077] This solution provides a device capable of automatically adding and removing pipette tips, adding samples, and dispensing samples. Through a stepper motor and synchronous belt drive, the entire dispensing device moves horizontally to its preset positions (e.g., ① tip addition position, ② tip removal position, ③ sample addition position, ④ sample dispensing position). Vertical movement is achieved via the stepper motor, enabling tip addition and removal, insertion of the tip into the sample tube for addition, and close-range dispensing. It also features sample level detection, providing a precise and efficient fully automated process. This solution, when used with an integrated reagent card and disposable pipette tips, enables automatic addition and installation of disposable tips; automatic removal of used tips; automatic sample addition and dispensing; high-precision dispensing based on sample level; real-time monitoring of tip status; and simultaneous independent movement of up to five sets of pipette tips for dispensing.

[0078] Each stepper motor is paired with a set of linear guides, and together with independent motor drive boards, enables the independent movement of the five sets of pipette tips. The drive boards have built-in encoders that, in conjunction with corresponding photoelectric switches, achieve closed-loop control, improving the overall motion accuracy of the device. Each set of pipette tips is equipped with a liquid level detection plate, which, when used with conductive pipette tips, allows for precise sample aspiration of different sample volumes, further enhancing the overall sampling accuracy and performance of the instrument. Real-time monitoring of the pipette tip status further strengthens the instrument's intelligence.

[0079] The synchronous belt and pressure block are connected in series to transmit the power source, thereby enabling the horizontal movement of the nozzle 59 and nozzle mounting plate 56 in the XY plane. Vertical movement is then achieved by the lead screw stepper motors in the nozzle 59 and nozzle mounting plate 56, thus moving the sample application device to its preset positions within the mechanism.

[0080] This solution enables automatic tip addition and removal; automatic sample addition and sorting; conductive tips equipped with a liquid level detection system precisely control the depth of tip insertion below the sample liquid surface, thus ensuring the sample addition accuracy requirements of the entire device; five sets of tips can move independently of each other, allowing up to five sets of tips to move independently simultaneously, thereby improving the efficiency of the entire device; the status of the tips can be monitored in real time, and when a tip malfunctions (such as failure to add, failure to remove, or falling off during operation), timely feedback is provided, and corresponding corrections are made when necessary, making the entire device more intelligent; the tips can move along the XYZ axes within the internal space of the mechanism, greatly improving the flexibility of the instrument and increasing work efficiency.

[0081] Example 7:

[0082] like Figure 7As shown, the incubation module includes an incubation data acquisition mechanism and a waste card strip removal mechanism. The incubation data acquisition mechanism includes an incubation base plate 77, with multiple incubation columns 76 installed at the bottom of the incubation base plate 77 for support. Channels for installing reagent card strips are provided on the incubation base plate 77, and the reagent card strips are installed in the corresponding channels. In this embodiment, up to 30 reagent card strips can be incubated at one time, improving testing efficiency.

[0083] A drive mechanism fourteen 75, a synchronous pulley, and a synchronous belt seven 73 are provided on the incubation base plate 77. The driving pulley and the driven pulley are both mounted on the incubation base plate 77 and can rotate around their own axis. The synchronous belt is connected to both the driving pulley and the driven pulley. The driving pulley is connected to the drive mechanism fourteen 75, and the data acquisition device is connected to the synchronous belt. The data acquisition device can move horizontally under the drive mechanism fourteen 75 through the action of the synchronous belt.

[0084] A guide rail 12 71 is mounted on the incubation base plate 77. The data acquisition unit is connected to the guide rail 12 71 and can move along the guide rail 12 71. In this way, the data acquisition unit achieves movement and limitation through the synchronous belt and the guide rail 12 71, and the entire movement trajectory is defined, thereby making the working process controllable. An incubation scale 72 is also provided on the incubation base plate 77. A heating plate and a temperature sensor are provided on the incubation base plate 77, and the temperature sensor is connected to the heating plate. The heating plate is preferably made of a heating aluminum substrate. An optocoupler 2 74 is also provided on the incubation base plate 77.

[0085] The data acquisition unit is also connected to a cable protection drag chain 70 to protect the cable during movement. The data acquisition unit includes an optical module 78, which is mounted on a guide rail 71 and can move along it. A drive mechanism 69 is installed on the optical module 78, and the output of the drive mechanism 69 is connected to a PMT photomultiplier tube. The drive mechanism 69 can drive the PMT photomultiplier tube to move horizontally to acquire data from the reagent strips.

[0086] The waste card rejection mechanism and the incubation data acquisition mechanism are located on the same straight line. This allows the transport module to move in the same direction, enabling coordinated operation of both mechanisms. The waste card rejection mechanism includes a pusher plate 79, on which a drive mechanism sixteen 68, a synchronous pulley, and a synchronous belt six 67 are mounted. Both the driving and driven pulleys of the synchronous pulley are mounted on the pusher plate 79 and can rotate around their own axes. The synchronous belt is connected to both the driving and driven pulleys. The driving pulley is connected to the drive mechanism sixteen 68 and then to the pusher fork 80 via the synchronous belt. The drive mechanism sixteen 68 drives the synchronous belt, causing the pusher fork 80 to move horizontally. A guide rail thirteen is mounted on the pusher plate 79, and the pusher fork 80 is connected to and can move along the guide rail thirteen. Through the combined action of the guide rail thirteen and the synchronous belt, movement and limiting are achieved, thus defining the entire movement trajectory and making the working process controllable. The waste card baffle 65 and the suction head exit bracket 66 are also located on the supporting base plate 8. The main function of the waste card strip rejection mechanism is to reject waste card strips after testing.

[0087] After the transport module transports the reagent card strip to the incubation module, the reagent card undergoes a reagent reaction. Once the reaction is complete, the light-collecting module 78, under the combined action of drive mechanism 15 69 and drive mechanism 14 75, can achieve lateral and longitudinal movement, ultimately enabling light collection on reagent cards at different incubation positions. The transport module then transports the light-collected reagent card strip to a preset position on the push plate 79. Under the combined action of drive mechanism 16 68 and push fork 80, the reagent card is pushed into the waste card baffle 65, and the reagent card falls down the inclined plane. The pipette moves to the preset position for retracting the pipette tip, retracting the waste pipette tip into the tip retraction bracket 66, where it falls down the inclined plane. Combined with the incubation scale 72, closed-loop control can be achieved to improve the overall movement accuracy of the module.

[0088] This solution provides a method for automatically heating and incubating reagent strips, and automatically rejecting discarded reagent strips. Through a stepper motor and synchronous belt drive, it achieves automatic acquisition of reagent luminescence data and automatic rejection of discarded strips. An incubation partition automatically fixes and positions the strips. A heated PCB board and temperature sensor provide real-time feedback and control of the incubation temperature. This solution uses a stepper motor paired with a linear guide rail and independent motor drive boards to achieve independent movement of the discarded strip pusher and the PMT (Pressure Handling Machine). This allows the PMT to be moved arbitrarily above the strips requiring data acquisition, and the rejection of discarded strips and data acquisition are independent and do not interfere with each other, thus improving testing efficiency.

[0089] The rejecting mechanism and the incubation mechanism are relatively independent, and the transfer of cards between them is accomplished through a transport component. Rejection and heating / incubation can operate simultaneously, improving testing efficiency. In other words, the transport mechanism enables the transfer and reaction testing of cards between the rejection and incubation mechanisms.

[0090] This solution can incubate up to 30 reagent strips at a time, improving testing efficiency; its incubation temperature can be fed back and controlled in real time; the PMT can randomly read the luminescence data of reagent strips in any numbered compartment; the incubation compartment and the rejection mechanism work separately and independently without interfering with each other.

[0091] Example 8:

[0092] like Figure 8 As shown, the control module 6 consists of a main control board 2 81, a mounting board 3 82, a drive board 83, a drive board 2 84, a drive board 3 85, and a switch 86. These structures enable precise control of the working process. These are all existing structures that can be purchased directly.

[0093] Example 9:

[0094] like Figure 9 As shown, the transport mechanism includes a transport plate 1, which is connected to a drive mechanism 8 91 and can move horizontally under the action of the drive mechanism 8 91. A drive mechanism 17 90 and a transport plate 2 are mounted on the transport plate 1 and are connected. The transport plate 2 moves horizontally relative to the transport plate 1 under the action of the drive mechanism 17 90. A transport fork 95 and a drive mechanism 9 are mounted on the transport plate 2. The transport fork is connected to the drive mechanism 9 and can move vertically under the action of the drive mechanism 9, and is connected to a hook mechanism. The transport fork 95 can move vertically under the drive of the drive mechanism 9 94, move longitudinally along the linear guide rail 14 93 under the drive of the drive mechanism 17 90, and move laterally along the linear guide rail 15 97 under the drive of the synchronous pulley and synchronous belt 8 87 driven by the drive mechanism 8 91. Ultimately, the reagent cards can be transported to various preset positions and retrieved from various preset positions in the incubation module. The addition of sensor 389 enables monitoring of the reagent card's status, while the use of code teeth 96 and optocoupler 392 allows for closed-loop control, improving the overall module's motion accuracy. Cable protection cable chain 388 is used for cable protection.

[0095] Example 10:

[0096] like Figure 10 and Figure 11As shown, the reagent cartridge module includes a cartridge holder, which consists of a bottom plate 99, side plates 114, a rear plate 109, and a top cover 113, forming a hollow internal box structure. The bottom plate 99 is supported by a column 98. One side wall of the cartridge holder has an opening through which the reagent cartridge 100 enters and exits. A drive mechanism 18 111 is installed on the side wall of the cartridge holder. The drive mechanism 18 111 is connected to a timing pulley and a timing belt 110. The scanning device 115 is connected to the timing belt. The movement of the drive mechanism 18 111 moves the timing belt, thereby moving the scanning device 115 to read information from reagent cartridges at different positions. The side wall of the cartridge holder with the scanning device 115 has multiple holes, each corresponding to a reagent cartridge 100, for easy reading. A detection plate 112 is also installed on the side wall of the cartridge holder.

[0097] In order to limit the movement trajectory of the scanning device 115, a guide rail 16 116 is provided on the side wall of the cartridge, and the scanning device 18 is connected to the guide rail 16 116 and can move along the guide rail 16 116.

[0098] The hook-and-clamp mechanism includes a hook-and-clamp base plate 107 and a hook-and-clamp device. The hook-and-clamp device is mounted on the hook-and-clamp base plate 107, which is located below the card holder. A drive mechanism 19 101, a timing pulley, and a timing belt 9 108 are mounted on the hook-and-clamp base plate 107. The drive mechanism 19 101, the timing pulley, and the timing belt 9 108 are connected to form a transmission mechanism. The hook-and-clamp device is connected to the timing belt, allowing it to move horizontally under the action of the drive mechanism 19 101. A guide rail 17 102 is provided on the hook-and-clamp base plate 107, and the hook-and-clamp device is connected to and can move along the guide rail 17 102. Hook-and-clamp teeth 103 are also provided on the hook-and-clamp base plate 107.

[0099] The hook clamp includes a horizontal hook clamp plate 117 and a vertical hook clamp. The horizontal hook clamp plate 117 is connected to guide rail seventeen 102 and can move along guide rail seventeen 102. The vertical hook clamp is mounted on the horizontal hook clamp plate 117. A drive mechanism twenty 106, a synchronous pulley, and a synchronous belt eleven 118 are provided on the horizontal hook clamp plate 117. The drive mechanism twenty 106, the synchronous pulley, and the synchronous belt eleven 118 are connected to form a transmission mechanism. The vertical hook clamp is connected to the synchronous belt, so the vertical hook clamp can move horizontally under the action of the drive mechanism twenty 106 and pulled by the synchronous belt. A guide rail eighteen is provided on the horizontal hook clamp plate 117, and the vertical hook clamp is connected to the guide rail eighteen and can move along the guide rail. The drive mechanism twenty 106 is connected to a cable protection drag chain four 104. The drive mechanism eighteen 111, drive mechanism twenty 106, and drive mechanism nineteen 101 are preferably stepper motors.

[0100] The vertical hook clamp includes a mounting plate, a hook clamp fork 119, and a drive mechanism 21 105. The mounting plate is connected to and can move along the guide rail 18. The drive mechanism 21 105 is located on the mounting plate, and the hook clamp fork 119 is connected to the drive mechanism 21 105. Under the action of the drive mechanism 21 105, the hook clamp fork 119 can move vertically and can be inserted into the clamp holder. The drive mechanism 21 105 is preferably a lead screw motor. The lead screw of the lead screw motor and the end of the hook clamp fork 119 form a lead screw and nut pair structure, which converts the rotation of the drive mechanism 21 105 into the vertical movement of the hook clamp fork 119.

[0101] Explanation of the principles behind this solution:

[0102] Reagent card holders 100 are placed in their preset positions, and a detection plate 112 monitors their status in real time. The hook fork 119 moves vertically under the drive of drive mechanism 105, longitudinally under drive mechanism 106, and laterally along linear guide rail 102 under drive mechanism 191. This allows for the hooking of reagent cards from each holder 100 and placement into the transport module. The hook teeth 103 further enable closed-loop control to improve the overall module's motion accuracy. The scanning device 115 moves laterally under the drive of drive mechanism 18111, thus reading information from each holder 100. This invention allows for the simultaneous placement of multiple reagent card holders for different testing items; automatic detection of reagent card holder status; automatic reading of reagent card holder information; and automatic hooking of reagent card strips.

[0103] The principle of this invention is as follows:

[0104] The process involves: placing reagent cartridges for different items into the reagent cartridge module and reading the reagent cartridge information; placing pipette tips into the pipette tip module; placing the sample to be tested into the sample module; entering the test information of the sample to be tested into the testing software and clicking "Start Test"; the transport module hooking the reagent card strip for the corresponding item; transporting it to the puncture position for membrane breaking; transporting it to the sample dispensing position; adding pipette tips into the sample dispensing module; reading the sample information into the sample module; the cap removal module moving the sample tube to the shaking module for shaking; removing the test tube cap; the sample dispensing module drawing up the sample; dispensing it into the reagent card strip for dilution and mixing; dispensing it into the reagent card strip dispensing port; the transport module transporting it to the incubation module for incubation; the light collection module collecting light; the transport module removing the reagent card strip that has been collected by light; transporting it to the card discarding position for card discarding; the sample dispensing module removing the pipette tips; the cap removal module closing the test tube cap; transporting the sample tube to the test tube rack; and the sample module removing the tested sample. This invention enables automatic sample loading and unloading and sample information reading; automatic sample tube handling, cap removal and cap replacement; automatic sample mixing; automatic pipette tip addition, removal and status detection; simultaneous multi-sample testing; automatic sample dilution and mixing; automatic reagent strip pickup and transfer; mobile and dynamic light acquisition; and sample level detection. Its modular design allows for faster and more flexible integration of the entire system.

[0105] The above specific embodiments further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above are merely specific embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A dry fluorescence immunoassay analyzer for multi-channel and automated detection, comprising a rack, characterized in that: The frame is equipped with a sample module, a mixing module, a pipette tip module, a cap removal module, a sample dispensing module, an incubation module, a transport module, and a reagent cartridge module. The transport module is connected to both the reagent cartridge module and the incubation module. The sample dispensing module is located above the transport module's movement path and is connected to the pipette tip module. The cap removal module is connected to the mixing module, and the sample module is connected to both the cap removal module and the mixing module. The sample module includes a sample rack base plate, which is fixed to the frame. A sample rack is mounted on the sample rack base plate and is located below and movably connected to the cap removal module and the mixing module. A drive mechanism is located below the sample rack base plate and is connected to an injection fork, which is connected to the sample rack. The drive mechanism operates as follows: The lower sample rack and the sample infeed fork can move horizontally relative to the sample rack base plate. A transverse base plate is provided outside the sample rack base plate, on which a second drive mechanism and a scanner are mounted. After the sample rack moves under the action of the first drive mechanism, it can connect with the second drive mechanism, and under the action of the second drive mechanism, it moves horizontally relative to the sample rack base plate and passes through the scanner station. A third drive mechanism is provided below the sample rack base plate, and the third drive mechanism is connected to a sample outlet fork. The outlet fork can connect after the sample rack moves with the second drive mechanism, and under the action of the third drive mechanism, the sample rack can move horizontally relative to the sample rack base plate. The shaking module includes a support mechanism, which is fixed to the frame. A shaking body and a fourth drive mechanism are provided on the support mechanism. The driving mechanism four and the support mechanism are fixed. The shaking body is simultaneously connected to the support mechanism and the driving mechanism four. The shaking body can rotate around the connection point of the support mechanism under the drive mechanism four. The sample holder and the cap removal module are movably connected to the shaking body. A clamping mechanism and a driving mechanism five are provided below the shaking body. The clamping mechanism can move relative to the support mechanism under the drive mechanism five to achieve clamping or releasing actions. The clamping mechanism includes a shaking clamp seat, a pressure block, and a linear guide rail five. The shaking clamp seat and the linear guide rail five are connected. The linear guide rail five is fixed on the support mechanism. The pressure block and the shaking clamp seat are fixed. The pressure block and the shaking body are elastically connected. The shaking clamp seat can move along the linear guide rail five. The shaking clamp seat and the driving mechanism five are connected. The system includes a clamping mounting plate with rollers. Under the action of the driving mechanism, the clamping mounting plate moves linearly, causing the rollers to enter between the pressure block and the shaking clamp, where they contact the shaking clamp. The cap-removing module includes a horizontal plate frame, which is fixed to the machine frame. A moving mechanism and several grippers are mounted on the horizontal plate frame. Each gripper is connected to the moving mechanism and can move relative to the horizontal plate frame under its influence. The grippers are movably connected to the shaking body and the sample holder. A clamping mechanism is provided on the horizontal plate frame, connected to the grippers and clamping or loosening under its action. A rotating mechanism is also provided on the horizontal plate frame, connected to the grippers and allowing them to rotate around their own axis under its action.The suction head module includes a suction head box mounting plate, which is fixed to the frame. A suction head box is mounted on the mounting plate, and a drive mechanism six is ​​also mounted on the mounting plate. The drive mechanism six is ​​connected to the suction head box, and under the action of the drive mechanism six, the suction head box can move relative to the mounting plate. During the movement of the suction head box, it connects to the sample dispensing module. A drive mechanism seven is also mounted on the mounting plate, and a puncture head is connected to the drive mechanism seven. The puncture head moves vertically under the action of the drive mechanism seven. The sample dispensing module includes sample dispensing columns, which are fixed to the frame. A sample dispensing horizontal plate one is positioned between the sample dispensing columns and is fixed to the sample dispensing columns. A sample dispensing horizontal plate two is positioned between the sample dispensing horizontal plates one and can move along the sample dispensing horizontal plate one. The system is equipped with a multi-pipette, which can move along the sample application plate and can also move vertically to insert into the pipette tip box, the mixing body, or the sample holder. The incubation module includes an incubation data acquisition mechanism and a waste strip removal mechanism. A transport module is movable relative to these mechanisms and is movably connected to them. The incubation data acquisition mechanism is equipped with a heating plate and a temperature sensor, with the temperature sensor connected to the heating plate. The reagent cartridge module includes a cartridge holder with a scanning device mounted on it, which is movable relative to the cartridge holder. A hook mechanism is externally located on the cartridge holder, allowing it to move relative to and insert into the cartridge holder.

2. The dry fluorescence immunoassay analyzer based on multi-channel and automated detection according to claim 1, characterized in that: The transportation module includes a transportation mechanism and a drive mechanism eight. The drive mechanism eight is connected to the transportation mechanism and can drive the transportation mechanism to move horizontally.

3. The dry fluorescence immunoassay analyzer based on multi-channel and automated detection according to claim 2, characterized in that: The transport mechanism includes a transport plate 1, which is connected to a drive mechanism 8 and can move horizontally under the action of the drive mechanism 8. A drive mechanism 17 and a transport plate 2 are provided on the transport plate 1, and the drive mechanism 17 and the transport plate 2 are connected. The transport plate 2 moves horizontally relative to the transport plate 1 under the action of the drive mechanism 17. A transport fork and a drive mechanism 9 are provided on the transport plate 2. The drive mechanism 9 is installed on the transport plate 2, and the transport fork is connected to the drive mechanism 9. The transport fork can move vertically under the action of the drive mechanism 9 and is connected to a hook mechanism.

Images