Sample addition method for gynecological secretion detection

Abstract

The application discloses a sample adding method for gynecological secretion detection, which comprises the following steps: moving a sample adding mechanism to a sample suction position to suck a sample; moving the sample adding mechanism from the sample suction position to a detection position and adding the sample to a turbidity detection container; sucking the sample after turbidity detection is completed by the sample adding mechanism and moving the sample adding mechanism from the detection position to a dyeing position to add the sample after turbidity detection is completed to a dyeing container; sucking the sample after dyeing is completed by the sample adding mechanism and moving the sample adding mechanism from the dyeing position to the sample adding position to add the sample after dyeing is completed to a detection card. Since turbidity detection and dyeing are arranged at different positions, i.e. turbidity detection and dyeing are respectively performed in different containers, the influence of residual dyeing agent on turbidity detection in a cyclic detection process can be avoided, and thus the detection precision is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of gynecological secretion detection, and particularly relates to a sample adding method for gynecological secretion detection. BACKGROUND

[0002] Gynecological secretion detection can help women understand their own physical condition and play a role in diagnosing and preventing diseases. Gynecological secretion detection mainly performs morphological detection, which requires turbidity detection and staining of the sample. At present, dilution and staining are performed in one container in gynecological secretion detection instruments. In the process of cyclic detection, residues of the staining agent can easily affect the accuracy of turbidity detection, and ultimately affect the detection accuracy. SUMMARY

[0003] The present application provides a sample adding method for gynecological secretion detection with high detection efficiency.

[0004] In one embodiment, a sample adding method for gynecological secretion detection is provided, comprising the following steps:

[0005] The sample adding mechanism moves to the sample suction position to suck the sample;

[0006] The sample adding mechanism moves from the sample suction position to the detection position and adds the sample to the turbidity detection container;

[0007] The sample adding mechanism sucks the sample after turbidity detection and moves from the detection position to the staining position to add the sample after turbidity detection to the staining container;

[0008] The sample adding mechanism sucks the sample after staining and moves from the staining position to the sample adding position to add the sample after staining to the detection card.

[0009] Further, the step of the sample adding mechanism moving from the sample suction position to the detection position and adding the sample to the turbidity detection container comprises the step of the sample adding mechanism moving from the sample suction position to the detection position and adding a part of the sample to the container;

[0010] After the step of the sample adding mechanism moving from the sample suction position to the detection position and adding a part of the sample to the turbidity detection container, and before the step of the sample adding mechanism sucking the sample after turbidity detection and moving from the detection position to the staining position to add the sample after turbidity detection to the staining container, the method further comprises the following steps:

[0011] The sample adding mechanism moves from the detection position to the sample adding position and adds another part of the sample to the detection card;

[0012] The sample adding mechanism retreats from the sample adding position to the detection position.

[0013] Further, the step of moving the sample adding mechanism from the sample adding position to the detection position includes:

[0014] The sample adding mechanism moves from the sample adding position to the detection position to perform a mixing operation on the diluted sample after the turbidity detection is completed.

[0015] Further, the step of the sample adding mechanism sucking the sample after the staining is completed and moving from the staining position to the sample adding position to add the sample after the staining to the detection card includes:

[0016] The sample adding mechanism performs a mixing operation on the sample after the staining.

[0017] The sample adding mechanism sucks the sample after the mixing is completed and moves from the staining position to the sample adding position to add the sample after the staining to the detection card.

[0018] Further, the sample adding mechanism performs a mixing operation on the sample by using a sucking and spitting or stirring method.

[0019] Further, after the step of the sample adding mechanism adding the sample after the staining to the detection card, the sample adding mechanism moves to a cleaning position to perform cleaning.

[0020] Further, the detection card is provided with a dry chemical detection area and a morphological detection area, the sample adding mechanism moves from the detection position to the sample adding position and adds another part of the sample to the dry chemical detection area of the detection card, and the sample adding mechanism sucks the sample after the staining is completed and moves from the staining position to the sample adding position to add the sample after the staining to the morphological detection area of the detection card.

[0021] Further, the detection card includes a dry chemical detection card and a morphological detection card, the sample adding mechanism moves from the detection position to the sample adding position and adds another part of the sample to the dry chemical detection card, and the sample adding mechanism sucks the sample after the staining is completed and moves from the staining position to the sample adding position to add the sample after the staining to the morphological detection card.

[0022] Further, the sample sucking position, the detection position and the sample adding position are arranged in sequence along a first direction, and the staining position and the detection position are arranged along a second direction.

[0023] Further, the sample adding mechanism includes a sampling needle, a sample adding tube and a plunger pump, the sampling needle is connected with the plunger pump through the sample adding tube, and the plunger pump is used to drive the sampling needle to suck and discharge the sample.

[0024] According to the sample adding method for detecting gynecological secretion in the above embodiment, since the turbidity detection and the staining are arranged at different positions, i.e., the turbidity detection and the staining are respectively performed in different containers, the influence of the residual staining agent on the turbidity detection in the cyclic detection process can be avoided, and thus the detection accuracy is improved. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 FIG. 1 is a structural schematic diagram of a sample adding mechanism in an embodiment;

[0026] Figure 2 FIG. 2 is a trajectory path diagram of a sampling needle in an embodiment;

[0027] Figure 3 FIG. 3 is a flowchart of a sample adding method of a sampling needle in an embodiment;

[0028] Figure 4 FIG. 4 is a trajectory path diagram of a sampling needle in an embodiment;

[0029] Figure 5 FIG. 5 is a flowchart of a sample adding method of a sampling needle in an embodiment. DETAILED DESCRIPTION

[0030] The application will be further described in details through specific embodiments and the accompanying drawings. In different embodiments, similar elements are marked with similar element reference numbers. In the following embodiments, many details are described in order to make the application better understood. However, those skilled in the art can easily recognize that some features can be omitted in different cases, or can be replaced by other elements, materials or methods. In some cases, some operations related to the application are not shown or described in the specification in order to avoid the core part of the application being overwhelmed by too much description, and it is not necessary to describe these related operations in detail for those skilled in the art according to the description in the specification and the general technical knowledge in the art.

[0031] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate manner to form various embodiments. Meanwhile, the steps or actions in the method description can also be sequentially adjusted or adjusted in a manner that is obvious to those skilled in the art. Therefore, the order in the specification and the drawings is only for the purpose of clearly describing a certain embodiment, and does not mean that it is the necessary order, unless otherwise stated that a certain order must be followed.

[0032] In this paper, the serial numbers of components, such as "first", "second", etc., are only used to distinguish the described objects, and have no order or technical meaning. Unless otherwise specified, "connection" and "coupling" in this application include direct and indirect connection (coupling).

[0033] Embodiment one:

[0034] In an embodiment, a sample adding method for gynecological secretion detection is provided. The sample adding method is mainly used in the process of dry chemical detection and morphological detection of gynecological secretion. A sampling needle can add samples to a dry chemical detection area and a morphological detection area respectively for detection.

[0035] Referring to Figure 1 The sample adding method in the embodiment is mainly realized by a sample adding mechanism in a gynecological secretion detection device. The sample adding mechanism includes a sampling needle 11, a sample adding pipe 12, and a plunger pump 13. The sampling needle 11 is connected to the plunger pump 13 through the sample adding pipe 12. The plunger pump 13 drives the sampling needle 11 to suck and discharge samples through negative pressure and positive pressure. The sample adding pipe 12 serves to connect the sampling needle 11 and the plunger pump 13. The plunger pump 13 and the sample adding pipe 12 also serve to buffer samples.

[0036] Referring to Figure 2 The gynecological secretion detection device in the embodiment mainly includes a sample sucking position A, a detection position B, a sample adding position, a dyeing position D, and a cleaning position F. The sample adding position includes a sample adding position C and a sample adding position E. The sample sucking position A is used to place a sample container 20 for the sampling needle 11 to suck samples. The detection position B is used to place a turbidity detection container 30 for sample dilution treatment. The sample adding position C is a dry chemical detection area of a detection card. The dyeing position D is used to place a dyeing container 40 for sample dyeing treatment. The sample adding position E is a morphological detection (microscopic examination) area. The cleaning position F is used to place a cleaning pool 50 for cleaning the inner and outer walls of the sampling needle 11. The sampling needle 11 is installed on a sample adding moving mechanism. The sample adding moving mechanism can drive the sampling needle 11 to move between the sample sucking position A, the detection position B, the sample adding position C, the dyeing position D, the sample adding position E, and the cleaning position F. The sample adding moving mechanism can also drive the sampling needle 11 to move up and down. The sample adding position C and the sample adding position E are located on the same detection card. In other embodiments, the sample adding position C and the sample adding position E can be located on different detection cards, such as the sample adding position C being located on a dry chemical detection card and the sample adding position E being located on a morphological detection (microscopic examination) card.

[0037] In the embodiment, the dyeing position D and the sample adding position E are arranged side by side. The sample sucking position A, the detection position B, and the sample adding position C (or the sample adding position E) are arranged along a first direction. The detection position B and the dyeing position D are arranged along a second direction. The first direction and the second direction are perpendicular or approximately perpendicular to each other. This allows the sampling needle 11 to return from the sample adding position C to the detection position B to travel a relatively short distance, thereby improving the sample adding efficiency of the sampling needle 11.

[0038] Referring to Figure 2 and Figure 3 The sample adding method for gynecological secretion detection in the embodiment mainly includes the following steps:

[0039] S11: sample suction;

[0040] The sample taken by the cotton swab or swab is placed in the sample container 20, and after the sample container 20 is placed in the sample suction position A, the elution needle injects the eluent into the sample container 20 to elute the sample on the cotton swab or swab into the sample container 20;

[0041] After the sample elution is completed, the sample adding moving mechanism drives the sampling needle 11 to move to the sample suction position A and drives the sampling needle 11 to be inserted into the sample container 20; the plunger pump 13 drives the sampling needle 11 to suck the quantitative sample in the sample container 20 by negative pressure, and the quantitative sample sucked is stored in the sample adding tube 12 and the plunger pump 13, and a small part is also stored in the sample container 20; the sample adding moving mechanism drives the sampling needle 11 to move upward and away from the sample container 20, and the sample suction is completed.

[0042] Before the sampling needle 11 sucks the sample, the eluted sample can also be stirred by the stirring rod to make the sample sucked by the sampling needle 11 more uniform, so as to improve the accuracy of detection.

[0043] S12: add to the turbidity detection cup;

[0044] After the sampling needle 11 sucks the sample, the sample adding moving mechanism drives the sampling needle 11 to move to the detection position B and drives the sampling needle 11 to be inserted into the turbidity detection container 30 on the detection position B;

[0045] The sampling needle 11 adds a part of the sample (to be dyed sample) to the turbidity detection container 30 under the positive pressure of the plunger pump 13, and the sample adding moving mechanism drives the sampling needle 11 to move upward and away from the turbidity detection container 30, and the adding of the reaction container 30 is completed.

[0046] After the sampling needle 11 moves upward and away from the turbidity detection container 30, the detection mechanism detects the turbidity of the sample in the turbidity detection container 30, and if the turbidity of the sample is too high, the diluent is added to the turbidity detection container 30 by the adding mechanism to dilute the sample; if the turbidity of the sample is not high, no dilution treatment is performed.

[0047] S13: add the sample to the detection card in the sample adding position C;

[0048] After the sampling needle 11 adds a part of the sample (to be dyed sample) to the turbidity detection container 30, the sample adding moving mechanism drives the sampling needle 11 to move to the sample adding position C;

[0049] The sampling needle 11 adds another part of the sample (to be colored sample) to the dry chemical detection area of the detection card in the sample adding position C under the positive pressure of the plunger pump 13.

[0050] After the sample needle 11 adds sample, the display mechanism injects the color developing agent to the dry chemical detection area of the detection card to develop the sample to be developed, and then the photographing device is used for photographing detection, so as to perform dry chemical detection.

[0051] S14: sample suction;

[0052] After the sample needle 11 adds sample to the dry chemical detection area of the detection card corresponding to the sample adding position C, the sample adding moving mechanism drives the sample needle 11 to return to the detection position B and is inserted into the turbidity detection container 30 on the detection position B to suck the sample to be dyed after dilution or without dilution.

[0053] In other embodiments, before the sample needle 11 sucks the sample to be dyed after dilution or without dilution, the sample to be dyed after dilution in the turbidity detection container 30 is mixed. The mixing operation can be achieved by alternatingly driving the sample needle 11 to suck and spit the sample to be dyed through the positive and negative pressures of the plunger pump 13, or can be achieved by stirring the sample needle 11 driven by the sample adding moving mechanism. The mixing operation can improve the uniformity of sample suction, so as to improve the accuracy of detection.

[0054] S15: adding to the dyeing cup;

[0055] The sample adding moving mechanism drives the sample needle 11 to move to the dyeing position D and is inserted into the dyeing container 40 on the dyeing position D to add the sample to be dyed after dilution or without dilution into the dyeing container 40. After the sample needle 11 adds sample to the dyeing container 40, the dyeing mechanism adds the dyeing agent into the dyeing container 40.

[0056] After the dyeing agent is added, the sample adding moving mechanism drives the sample needle 11 to be inserted into the dyeing container 40 to mix the dyed sample. The mixing operation can be achieved by alternatingly driving the sample needle 11 to suck and spit the sample through the positive and negative pressures of the plunger pump 13, or can be achieved by stirring the sample needle 11 driven by the sample adding moving mechanism. The mixing operation can improve the uniformity of sample suction, so as to improve the accuracy of detection.

[0057] S16, adding sample to the detection card at the sample adding position E;

[0058] After the mixing operation, the sample needle 11 sucks the mixed dyed sample, the sample adding moving mechanism drives the sample needle 11 to move to the sample adding position E, and under the action of the positive pressure of the plunger pump 13, the sample needle 11 adds the sucked dyed sample to the morphological detection area of the detection card corresponding to the sample adding position E.

[0059] The dyed sample is spread on the detection card, and then the microscope mechanism is used for photographing the dyed sample on the detection card, so as to perform morphological detection (microscopic examination).

[0060] S17, cleaning.

[0061] After the sampling needle 11 is added to the adding position E, the adding moving mechanism drives the sampling needle 11 to move to the cleaning position F, the cleaning mechanism injects cleaning liquid into the cleaning pool 50 and injects cleaning liquid into the sampling needle 11, and the inner and outer walls of the sampling needle 11 are cleaned. After cleaning is completed, the sampling needle 11 enters the next cycle of sample adding operation.

[0062] In other embodiments, during the upward movement of the sampling needle 11 after the sample is sucked from the sample container 20, the cleaning swab cleans the outer wall of the sampling needle 11, avoiding the falling of the residual sample on the outer wall of the sampling needle 11 during the movement to pollute the machine environment.

[0063] The sample adding method for gynecological secretion detection provided in the embodiment can avoid the influence of the residual dyeing agent on the turbidity detection during the cyclic detection, thereby improving the detection accuracy, because the dilution and dyeing are arranged at different positions, that is, the dilution and dyeing are respectively performed in different containers.

[0064] Embodiment two:

[0065] The sample adding method for gynecological secretion detection provided in the embodiment is different from the above-mentioned embodiments in that the sample adding method only performs morphological detection, and the dry chemical detection is omitted, that is, the adding position C is omitted.

[0066] Please refer to Figure 4 The sampling needle 11 walks through a circular path to complete one cycle of adding, and the sample adding efficiency of the sampling needle 11 is improved.

[0067] Please refer to Figure 5 The sample adding method for gynecological secretion detection provided in the embodiment mainly includes the following steps:

[0068] S21: sample suction;

[0069] The cotton swab or swab sample is placed in the sample container 20, and after the sample container 20 is placed at the sample suction position A, the elution needle injects the eluent into the sample container 20 to elute the sample on the cotton swab or swab into the sample container 20;

[0070] After the sample elution is completed, the adding moving mechanism drives the sampling needle 11 to move to the sample suction position A, and drives the sampling needle 11 to be inserted into the sample container 20; the plunger pump 13 drives the sampling needle 11 to suck the quantitative sample in the sample container 20 by negative pressure, and the quantitative sample is stored in the adding tube 12 and the plunger pump 13, and a small part is also stored in the sample container 20; the adding moving mechanism drives the sampling needle 11 to move upward and away from the sample container 20, and the sample suction is completed.

[0071] Before the sampling needle 11 sucks the sample, the eluted sample can also be stirred by the stirring rod, so that the sample sucked by the sampling needle 11 is more uniform, thereby improving the accuracy of detection.

[0072] S22: filling to the turbidity detection container;

[0073] After the sampling needle 11 sucks the sample, the sampling moving mechanism drives the sampling needle 11 to move to the detection position B and drives the sampling needle 11 to be inserted into the turbidity detection container 30 on the detection position B;

[0074] The sampling needle 11 sucks the sample under the positive pressure of the plunger pump 13 and fills the sample to the turbidity detection container 30, and then the sampling moving mechanism drives the sampling needle 11 to move upward and away from the turbidity detection container 30, thereby completing the filling of the turbidity detection container.

[0075] After the sampling needle 11 moves upward and away from the turbidity detection container 30, the detection mechanism detects the turbidity of the sample in the turbidity detection container 30, and if the turbidity of the sample is too high, the diluent is filled into the turbidity detection container 30 by the filling mechanism to dilute the sample; if the turbidity of the sample is not high, no dilution treatment is performed.

[0076] S23: sucking the sample;

[0077] After the turbidity detection is completed, the sampling needle 11 sucks the sample in the turbidity detection container 30 after the dilution is completed or the sample that does not need to be diluted.

[0078] S24: filling to the dyeing cup;

[0079] The sampling moving mechanism drives the sampling needle 11 to move to the dyeing position D and be inserted into the dyeing container 40 on the dyeing position D, fills the sucked and diluted or not diluted sample to the dyeing container 40, and after the sampling needle 11 fills the dyeing container 40, the dyeing mechanism fills the dyeing agent to the dyeing container 40.

[0080] After the dyeing agent is filled, the sampling moving mechanism drives the sampling needle 11 to be inserted into the dyeing container 40 to perform the mixing operation on the dyed sample. The mixing operation can be achieved by alternating the positive and negative pressures of the plunger pump 13 to drive the sampling needle 11 to suck and spit the sample, or the mixing operation can be achieved by driving the sampling needle 11 to stir by the sampling moving mechanism. The mixing operation can improve the uniformity of the sample sucking, thereby improving the accuracy of detection.

[0081] S25: filling the sample to the detection card at the sampling position E;

[0082] After the mixing operation, the sampling needle 11 sucks the mixed and dyed sample, the sampling moving mechanism drives the sampling needle 11 to move to the sampling position E, and under the positive pressure of the plunger pump 13, the sampling needle 11 fills the sucked dyed sample to the morphological detection area of the detection card at the sampling position E.

[0083] The dyed sample is spread on the detection card, and the dyed sample on the detection card is photographed by a microscope to perform morphological detection (microscopic examination).

[0084] S26, cleaning.

[0085] After the sampling needle 11 is added to the adding position E, the adding moving mechanism drives the sampling needle 11 to move to the cleaning position F, the cleaning mechanism injects cleaning liquid into the cleaning pool 50 and into the sampling needle 11, and the inner and outer walls of the sampling needle 11 are cleaned. After cleaning is completed, the sampling needle 11 enters the next cycle of adding operation.

[0086] In other embodiments, during the upward movement of the sampling needle 11 after the sampling needle 11 absorbs the sample from the sample container 20, the cleaning swab cleans the outer wall of the sampling needle 11, avoiding the falling of the residual sample on the outer wall of the sampling needle 11 to pollute the machine environment during the movement.

[0087] In this embodiment, dilution and dyeing are also performed in two containers, which can avoid the influence of the residual dyeing agent on turbidity detection during the cycle detection, and further improve the detection precision.

[0088] The above application of specific examples is used to illustrate the present application, which is only used to help understand the present application, and does not limit the present application. For those skilled in the art to which the present application belongs, according to the idea of the present application, several simple deductions, deformations or substitutions can be made.

Claims

1. A method for sample application for gynecological discharge testing, characterized in that, The method comprises the following steps: The sample adding mechanism moves to the sample suction position to suck the sample; The sample adding mechanism moves from the sample suction position to the detection position and adds a part of the sample to the turbidity detection container; The sample adding mechanism moves from the detection position to the sample adding position and adds another part of the sample to the detection card; After the sample adding mechanism leaves the turbidity detection container, the sample in the turbidity detection container is detected by the detection mechanism, if the turbidity of the sample is too high, the sample is diluted by adding diluent to the turbidity detection container through the adding mechanism, if the turbidity of the sample is not too high, no dilution treatment is performed; The sample adding mechanism retreats from the sample adding position to the detection position, if the sample in the turbidity detection container is diluted, the diluted sample is mixed by the sample adding mechanism; The sample adding mechanism sucks the sample after turbidity detection and moves from the detection position to the dyeing position to add the sample after turbidity detection to the dyeing container; The sample adding mechanism sucks the sample after dyeing and moves from the dyeing position to the sample adding position to add the sample after dyeing to the detection card.

2. The sample application method for gynecological discharge detection according to claim 1, wherein, The step that the sample adding mechanism sucks the sample after dyeing and moves from the dyeing position to the sample adding position to add the sample after dyeing to the detection card comprises: The sample adding mechanism mixes the sample after dyeing; The sample adding mechanism sucks the sample after mixing and moves from the dyeing position to the sample adding position to add the sample after dyeing to the detection card.

3. The sample application method for gynecological discharge detection according to claim 1 or 2, characterized by, The sample adding mechanism mixes the sample by sucking and spitting or stirring.

4. The sample application method for gynecological discharge detection according to claim 1, wherein, After the step that the sample adding mechanism adds the sample after dyeing to the detection card, the sample adding mechanism moves to the cleaning position for cleaning.

5. The sample application method for gynecological discharge detection according to claim 1, wherein, The detection card is provided with a dry chemical detection area and a morphological detection area, the sample adding mechanism moves from the detection position to the sample adding position and adds another part of the sample to the dry chemical detection area of the detection card, and the sample adding mechanism sucks the sample after dyeing and moves from the dyeing position to the sample adding position to add the sample after dyeing to the morphological detection area of the detection card.

6. The sample application method for gynecological discharge detection according to claim 1, wherein, The detection card comprises a dry chemical detection card and a morphological detection card, the sample adding mechanism moves from the detection position to the sample adding position and adds another part of the sample to the dry chemical detection card, and the sample adding mechanism sucks the sample after dyeing and moves from the dyeing position to the sample adding position to add the sample after dyeing to the morphological detection card.

7. The sample application method for gynecological discharge testing according to claim 1, wherein, The sample suction position, the detection position and the sample adding position are arranged in sequence along a first direction, and the dyeing position and the detection position are arranged along a second direction.

8. The sample application method for gynecological discharge testing according to claim 1, wherein, The sample adding mechanism comprises a sampling needle, a sample adding tube and a plunger pump, the sampling needle is connected with the plunger pump through the sample adding tube, and the plunger pump is used to drive the sampling needle to suck and discharge the sample.

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