Sample analyzer and sample analysis method

Abstract

A sample analyzer and a sample analysis method, the sample analyzer comprises a sample bearing mechanism, a reagent bearing mechanism, a sample dispensing mechanism, a reagent dispensing mechanism, a reaction mechanism, a measuring mechanism, an ultrasonic device and a controller, the ultrasonic device is used for generating ultrasonic vibration to form ultrasonic waves, and the controller is used for controlling the ultrasonic device to emit ultrasonic waves into the incubated reaction solution. Since the sample analyzer is provided with the ultrasonic device, the ultrasonic device can emit ultrasonic waves into the reaction solution to mix it uniformly, which has the advantages of sufficient mixing and avoiding spilling of the reaction solution compared with the traditional mechanical mixing. In particular, the ultrasonic device is used for emitting ultrasonic waves into the incubated reaction solution, and the ultrasonic waves can separate the aggregated substances formed in the incubation process, so that each component in the incubated reaction solution is uniformly dispersed, thereby improving the accuracy of detection.

Description

Technical Field

[0001] This invention relates to in vitro testing equipment, specifically to a sample analyzer and a sample analysis method. Background Technology

[0002] In vitro diagnostics (IVD) refers to the use of human samples, such as blood and urine, outside the human body to obtain clinical diagnostic information, thereby determining diseases or bodily functions. Because IVD can quickly and accurately diagnose diseases in their early stages, it plays an increasingly important role in clinical medicine and related medical research.

[0003] In the process of testing human samples, it is usually necessary to mix the sample with the corresponding reagent to form a reaction solution, and then use a mixing device to homogenize the reaction solution to ensure that the sample and reagent react fully. Traditional mixing devices often use stirring and shaking methods, which can easily lead to splashing of the reaction solution during the mixing process and poor mixing effect, thus affecting the accuracy of the final test results. Summary of the Invention

[0004] One embodiment provides a sample analyzer, comprising:

[0005] A sample carrier mechanism, used to hold the sample;

[0006] A reagent-carrying mechanism is used to hold reagents.

[0007] A sample dispensing mechanism is used to draw samples from the sample carrying mechanism and dispense the samples into a receiving cup;

[0008] A reagent dispensing mechanism is used to draw reagents from the reagent carrying mechanism and dispense the reagents into a receiving cup;

[0009] The reaction mechanism is used to provide an incubation site for the reaction solution in the container, which is formed by mixing the sample and reagents.

[0010] A measuring device used to measure the reaction solution;

[0011] An ultrasonic device for generating ultrasonic vibrations to form ultrasonic waves; and

[0012] A controller is connected to the ultrasonic device, and the controller is used to control the ultrasonic device to emit ultrasonic waves into the incubated reaction solution.

[0013] In one embodiment, the controller is used to acquire test parameters and, based on the test parameters, select one of a set of preset ultrasonic modes to perform ultrasonic mixing on the reaction solution.

[0014] In one embodiment, the multiple ultrasound modes have different ultrasound intensities and / or ultrasound durations.

[0015] In one embodiment, the ultrasonic device includes an ultrasonic transducer, a transmission element, and a moving device. The ultrasonic transducer is used to generate ultrasonic vibrations. The transmission element has a first end and a second end. The first end of the transmission element is connected to the ultrasonic transducer, and the outer diameter of the second end of the transmission element is smaller than the inner diameter of the container. The moving device is connected to the ultrasonic transducer and is used to drive the ultrasonic transducer and the transmission element to move relative to the container. The second end of the transmission element can be inserted into the reaction liquid inside the container to emit ultrasonic waves into the reaction liquid inside the container.

[0016] In one embodiment, the ultrasonic device includes an ultrasonic transducer and a transmission element. The ultrasonic transducer is used to generate ultrasonic vibrations. The transmission element has a first end and a second end. The first end of the transmission element is connected to the ultrasonic transducer. The second end of the transmission element is used to abut against the outer wall of a container. The portion of the outer wall of the container that contacts the transmission element is the portion surrounding the reaction liquid, so as to transmit ultrasonic waves to the reaction liquid inside the container.

[0017] In one embodiment, the transfer member is a solid structure, and the outer diameter of the transfer member gradually decreases or decreases in a stepwise manner from the first end to the second end.

[0018] In one embodiment, the ultrasonic device is used to perform ultrasonic mixing on the incubated reaction solution within the reaction apparatus.

[0019] In one embodiment, the sample analyzer further includes a transfer mechanism, and a mixing position is provided outside the reaction mechanism. The transfer mechanism is used to transfer a container holding the incubated reaction solution to the mixing position, and the ultrasonic device is used to perform ultrasonic mixing on the incubated reaction solution located at the mixing position.

[0020] In one embodiment, the sample analyzer further includes a magnetic separation mechanism, which includes a cleaning solution dispensing structure, a magnetic separation structure, a liquid absorption structure, and a substrate dispensing structure. The cleaning solution dispensing structure is used to inject cleaning solution into the incubated reaction solution. The magnetic separation mechanism is used to perform magnetic separation on the reaction solution containing the injected cleaning solution. The liquid absorption structure is used to absorb the liquid after magnetic separation. The substrate dispensing structure is used to inject substrate into the reaction solution after magnetic separation.

[0021] In one embodiment, the ultrasonic device is further used to perform ultrasonic mixing on at least one of the sample, reagent, reaction solution to be incubated, reaction solution for injecting washing solution, and reaction solution for injecting substrate.

[0022] In one embodiment, a sample analysis method is provided, comprising the following steps:

[0023] The sample dispensing mechanism and the reagent dispensing mechanism respectively inject the sample and reagent into the container to form a reaction solution;

[0024] The reaction solution is incubated within the reaction apparatus;

[0025] The ultrasonic device emits ultrasonic waves into the incubated reaction solution;

[0026] The testing institution performs optical measurements on the reaction solution.

[0027] In one embodiment, the ultrasonic mixing operation of the ultrasonic device is controlled by the following steps:

[0028] Obtain test project parameters based on the project;

[0029] Based on the test parameters, one ultrasonic mode is selected from a variety of preset ultrasonic modes to perform ultrasonic mixing on the reaction solution.

[0030] In one embodiment, the multiple ultrasound modes have different ultrasound intensities and / or ultrasound durations.

[0031] In one embodiment, the ultrasonic device emits ultrasonic waves into the incubated reaction solution within the reaction mechanism.

[0032] In one embodiment, between the incubation of the reaction solution in the reaction apparatus and the emission of ultrasonic waves by the ultrasonic device into the incubated reaction solution, the following step is further included:

[0033] The transfer mechanism transfers the container holding the incubated reaction solution to the mixing position;

[0034] The ultrasonic device emits ultrasonic waves into the incubated reaction liquid at the mixing site.

[0035] In one embodiment, the sample dispensing mechanism and the reagent dispensing mechanism respectively inject the sample and reagent into the receiving cup to form a reaction solution, including the following steps:

[0036] The sample dispensing mechanism dispenses the sample into the receiving cup;

[0037] The reagent dispensing mechanism dispenses reagents into a container holding the sample, so that the sample and reagents are mixed to form a reaction solution to be incubated.

[0038] In one embodiment, the sample dispensing mechanism and the reagent dispensing mechanism respectively inject the sample and reagent into the receiving cup to form a reaction solution, including the following steps:

[0039] The reagent dispensing mechanism dispenses the reagent into the receiving cup;

[0040] The sample dispensing mechanism dispenses the sample into a container containing reagents, so that the sample and reagents are mixed to form a reaction solution to be incubated.

[0041] In one embodiment, between the sample dispensing mechanism and the reagent dispensing mechanism injecting the sample and reagent into the receiving cup respectively to form a reaction solution and the incubation of the reaction solution in the reaction mechanism, the following steps are also included:

[0042] The transfer mechanism transfers the container holding the reaction solution to be incubated to the mixing position;

[0043] The ultrasonic device emits ultrasonic waves into the reaction solution to be incubated at the mixing site.

[0044] The transfer mechanism transfers the ultrasonically cooled reaction solution onto the reaction mechanism.

[0045] In one embodiment, between the sample dispensing mechanism and the reagent dispensing mechanism injecting the sample and reagent into the container cup respectively to form a reaction solution and the reaction solution being incubated in the reaction mechanism, the following step is also included: the ultrasonic device emitting ultrasonic waves into the reaction solution to be incubated located in the reaction mechanism.

[0046] In one embodiment, before the reagent dispensing mechanism adds the reagent into the container cup, the following step is further included: the ultrasonic device emits ultrasonic waves into the reagent located within the reagent carrying mechanism.

[0047] In one embodiment, between the step of the reagent dispensing mechanism adding reagent to the container and the step of the sample dispensing mechanism adding sample to the container containing reagent, the following step is further included: the ultrasonic device emitting ultrasonic waves to the reagent added to the container.

[0048] In one embodiment, the reagent dispensing mechanism sequentially dispenses at least two reagents into the receiving cup in multiple steps.

[0049] After at least one step in the multi-step dispensing process of the reagent dispensing mechanism is completed, the ultrasonic device emits ultrasonic waves into the reaction liquid or reagent in the container cup.

[0050] In one embodiment, between the step of the ultrasonic device emitting ultrasonic waves into the incubated reaction solution and the step of the measuring mechanism performing photometric measurements on the reaction solution, the following step is further included:

[0051] The transfer mechanism transfers the container to the magnetic separation mechanism, which performs magnetic separation on the incubated reaction solution.

[0052] The substrate dispensing structure injects the substrate into the reaction solution after magnetic separation;

[0053] The transfer mechanism transfers the reaction solution containing the injected substrate back into the reaction mechanism, where the reaction solution containing the injected substrate is incubated.

[0054] According to the sample analyzer and sample analysis method of the above embodiments, since the sample analyzer is equipped with an ultrasonic device, the ultrasonic device can emit ultrasonic waves into the reaction liquid to mix it. Compared with traditional mechanical mixing, it has the advantages of thorough mixing and avoiding splashing of the reaction liquid. In particular, the ultrasonic device is used to emit ultrasonic waves into the incubated reaction liquid. The ultrasonic waves can separate the agglomerates formed in the reaction liquid during the incubation process, so that the components in the incubated reaction liquid are uniformly dispersed, thereby improving the accuracy of detection. Attached Figure Description

[0055] Figure 1 This is a schematic diagram of the structure of an immunoluminescence analyzer in one embodiment;

[0056] Figure 2 This is a structural block diagram of the control section of an immunoluminescence analyzer in one embodiment;

[0057] Figure 3 This is a schematic diagram of the structure of a vortex mixing device in one embodiment;

[0058] Figure 4 This is a schematic diagram of the structure of a contact ultrasound device in one embodiment;

[0059] Figure 5 This is a structural view of the transfer element in one embodiment;

[0060] Figure 6 This is a structural view of the transfer element in one embodiment;

[0061] Figure 7 This is a schematic diagram of a mobile device in one embodiment;

[0062] Figure 8 This is a schematic diagram of an ultrasonic device in one embodiment;

[0063] Figure 9 This is a schematic diagram of the structure of a non-contact ultrasonic device in one embodiment;

[0064] Figure 10 This is a schematic diagram of the structure of a non-contact ultrasonic device in one embodiment;

[0065] Figure 11 This is a side view of the clamping device in one embodiment;

[0066] Figure 12 This is a top view of the clamping device in one embodiment;

[0067] Figure 13This is a timing diagram of a sample analysis method in one embodiment;

[0068] Figure 14 This is a flowchart of a sample analysis method in one embodiment;

[0069] Figure 15 This is a comparison chart of test results between ultrasonic mixing and non-ultrasonic mixing during sample analysis in one embodiment.

[0070] Figure 16 This is a timing diagram of a sample analysis method in one embodiment;

[0071] Figure 17 This is a flowchart of a sample analysis method in one embodiment;

[0072] Figure 18 This is a timing diagram of a variation scheme in the sample analysis method of one embodiment;

[0073] Figure 19 This is a flowchart of a sample analysis method in one embodiment;

[0074] Figure 20 This is a timing diagram of a variation scheme in a sample analysis method in one embodiment. Detailed Implementation

[0075] In sample analyzers, after the sample and reaction solution are mixed to form the reaction solution, the reaction solution needs to be further mixed to ensure that the sample and reagents react fully. Traditionally, mechanical mixing methods such as stirring and shaking are used to mix the reaction solution. Mechanical mixing creates a rotating vortex in the reaction solution, and centrifugal force is used to mix the reaction solution. However, mechanical mixing is difficult to disperse tightly clustered substances in the reaction solution, resulting in insufficient mixing. In particular, after incubation, more clusters will form in the reaction solution, and traditional mechanical mixing is even less effective in dispersing the various components of the reaction solution, ultimately leading to low accuracy of the test results.

[0076] Therefore, this application employs an ultrasonic device to ultrasonically mix the incubated reaction solution. The ultrasonic device emits ultrasonic waves into the reaction solution, and the ultrasonic waves generate ultrasonic vibrations that drive the rapid flow and mixing of the reaction solution, ensuring uniform dispersion of all components. Furthermore, when the ultrasonic waves reach a certain driving power, ultrasonic cavitation can also be formed in the reaction solution. Ultrasonic cavitation refers to the phenomenon where, when the ultrasonic energy is sufficiently high, tiny bubbles existing in a liquid vibrate, grow, and continuously accumulate sound field energy under the action of an ultrasonic field. When the energy reaches a certain threshold, the cavitation bubbles collapse and close rapidly. Under the action of ultrasonic cavitation, the diffusion of various substances in the reaction solution can be greatly accelerated, further improving the mixing efficiency and the accuracy of the detection results.

[0077] The present invention will now be described in further detail with reference to specific embodiments and accompanying drawings. Similar elements in different embodiments are referred to by associated similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of this application. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, certain operations related to this application are not shown or described in the specification. This is to avoid overwhelming the core parts of this application with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.

[0078] Furthermore, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments. At the same time, the order of the steps or actions in the method description can be changed or adjusted in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and drawings are only for clearly describing a particular embodiment and do not imply a necessary order, unless otherwise stated that a particular order must be followed.

[0079] The serial numbers assigned to components in this document, such as "first" and "second," are used only to distinguish the described objects and have no sequential or technical meaning. The terms "connection" and "linkage" used in this application, unless otherwise specified, include both direct and indirect connections (linkages).

[0080] For chemiluminescence immunoassay analyzers, a one-step test in this invention refers to a test that requires only one incubation step. Conversely, a multi-step test refers to a test that requires multiple incubation steps. For example, a two-step test requires two incubation steps: first, the reagents for the first incubation step are added to the sample, then the first incubation is performed. After the first incubation time is reached, the reagents for the second incubation step are added, and the second incubation is performed. After the second incubation time is reached, magnetic separation is performed, and then the measurement is taken. Generally, for a multi-step test, magnetic separation is required after the final incubation step before measurement can be performed. However, whether magnetic separation is required after other incubation steps in a multi-step test, except for the final incubation step, depends on factors such as the type of test. For example, a two-step test project can be called a two-step two-separation test project if magnetic separation is required after incubation in the first step, and a two-step one-separation test project if magnetic separation is not required after incubation in the first step.

[0081] In one-step or multi-step testing projects, the type of reagents required for each incubation step or each incubation can be one or more, depending on factors such as the type of testing project. When one or more steps in a one-step or multi-step testing project require the addition of multiple types of reagents during incubation, such a testing project can be called a multi-component testing project.

[0082] In one embodiment, a sample analyzer is provided, which includes an ultrasonic device to ultrasonically mix the incubated reaction solution, thereby improving the accuracy of the test results. This sample analyzer can be a biochemical analyzer or an immunoassay analyzer; this embodiment uses a chemiluminescence immunoassay analyzer as an example.

[0083] Please refer to Figure 1 and Figure 2 The chemiluminescence immunoassay analyzer mainly includes an ultrasonic device 10, a sample carrying mechanism 21, a sample dispensing mechanism 22, a reagent carrying mechanism 31, a reagent dispensing mechanism 32, a reaction mechanism 40, a magnetic separation mechanism 50, and a controller 60. The sample carrying mechanism 21, sample dispensing mechanism 22, reagent carrying mechanism 31, reagent dispensing mechanism 32, reaction mechanism 40, magnetic separation mechanism 50, and ultrasonic device 10 are all mounted on a base 100. The controller 60 is mounted on the main unit of the base 100, or it can be mounted on the base 100 itself. The biochemical analyzer does not include the aforementioned magnetic separation mechanism.

[0084] The immunoluminescence analyzer also includes a cup-loading mechanism 71, a cup-throwing position 72, a first transfer mechanism 81, a second transfer mechanism 82, and a measurement mechanism 90, all mounted on the base 100.

[0085] The reaction mechanism 40 is located in the middle, and the reagent carrying mechanism 31, magnetic separation mechanism 50, ultrasonic device 10, cup-raising mechanism 71, cup-throwing position 72 and measuring mechanism 90 are respectively located around the reaction mechanism 40.

[0086] The cup-loading mechanism 71 is used to store unused container cups 103, also known as reaction cups. The cup-loading mechanism 71 also has a cup-moving function, which can move the container cups 103 from the storage position to the position to be grasped.

[0087] The first transfer mechanism 81 is a cup-grabbing mechanism. The first transfer mechanism 81 is used to transfer the container cup 103 on the upper cup mechanism 71 to the sample feeding position 101 near the reaction mechanism 40, and to transfer the container cup 103 on the sample feeding position 101 into the reaction mechanism 40.

[0088] The cup-discarding position 72 is located within the movement range of the first transfer mechanism 81. The cup-discarding position 72 is connected to the recycling box and is used to recycle the used container cup 103. The first transfer mechanism 81 is also used to transfer the detected container cup 103 on the reaction mechanism 40 to the cup-discarding position 72.

[0089] The sample carrier 21 is used to carry samples. In some examples, the sample carrier 21 may include a sample delivery module (SDM); in other examples, the sample carrier 21 may also be a sample tray, which includes multiple sample positions for placing sample tubes, and the sample tray can be rotated to the corresponding position, such as the position for the sample dispensing mechanism 22 to pick up the sample, by rotating its tray structure.

[0090] The sample dispensing mechanism 22 includes a sampling needle, a moving mechanism, and a driving pump. The moving mechanism drives the sampling needle to move between the sample carrying mechanism 21 and the sample dispensing position 101, and the driving pump provides power for the sampling needle to aspirate and dispense samples. The sample dispensing mechanism 22 is used to aspirate the sample from the sample tube on the sample carrying mechanism 21 and to dispense the aspirated sample into the receiving cup 103 on the sample dispensing position 101.

[0091] The reagent carrier 31 is used to carry reagents. In one embodiment, the reagent carrier 31 can be a reagent tray, which is arranged in a disc shape and has multiple positions for carrying reagent containers. The reagent carrier 31 can rotate and drive the reagent containers it carries to rotate, so as to rotate the reagent containers to a specific position, such as the position where the reagent is drawn up by the reagent dispensing mechanism 32. The number of reagent carriers 31 can be one or more.

[0092] The reagent dispensing mechanism 32 includes a reagent needle, a moving mechanism, and a driving pump. The moving mechanism drives the reagent needle to move in two or three dimensions between the reagent carrying mechanism 31 and the reaction mechanism 40. The driving pump provides power to the reagent needle for aspirating and dispensing reagents. The reagent dispensing mechanism 32 is used to aspirate the reagent from the reagent tube on the reagent carrying mechanism 31 and to add the aspirated reagent to the sample-containing container 103 on the reaction mechanism 40. The sample and reagent in the container 103 mix and react to form a reaction solution.

[0093] The reaction mechanism 40 is used to provide an incubation site for the reaction liquid. The reaction mechanism 40 can be a reaction plate, which is arranged in a disc shape and has one or more placement positions for placing reaction cups. The reaction plate can rotate and drive the reaction cups in its placement positions to rotate, which is used to manage the reaction cups and incubate the reaction liquid in the reaction cups within the reaction plate.

[0094] The magnetic separation mechanism 50 includes a cleaning fluid dispensing structure, a magnetic attraction structure, a liquid absorption structure, and a substrate dispensing mechanism. The cleaning fluid dispensing structure is used to add cleaning fluid to the incubated reaction solution, and the cleaning fluid is used to separate free substances in the incubated reaction solution. The magnetic attraction structure is used to perform a magnetic attraction operation on the reaction solution to which the cleaning fluid has been added, and the magnetic attraction structure is used to adsorb the reaction complex bound to the magnetic beads. The liquid absorption structure is used to discharge other components except for the reaction complex bound to the magnetic beads from the receiving cup 103. The substrate dispensing mechanism is used to add substrate to the reaction solution in the receiving cup 103, and the substrate reacts with the reaction complex in the reaction solution, and the substrate luminescently marks the reaction complex.

[0095] There are two magnetic separation mechanisms 50, which can work independently to improve the efficiency of the test.

[0096] A mixing position 102 is provided near the reaction mechanism 40 and the magnetic separation mechanism 50. Both the mixing position 102 and the sample addition position 101 are provided with cup holders for placing the container cup 103. The second transfer mechanism 82 is used to transfer the container cup 103 between the reaction mechanism 40, the magnetic separation mechanism 50 and the mixing position 102.

[0097] The measuring mechanism 90 is used to perform photometric measurements on the incubated reaction solution to obtain the sample's reaction data. For example, the measuring mechanism 90 detects the luminescence intensity of the reaction solution to be tested and calculates the concentration of the analyte in the sample through a calibration curve.

[0098] The base 100 is also equipped with a cleaning mechanism and a waste liquid aspiration mechanism. The cleaning mechanism is used to clean the sampling needle and reagent needle, and the waste liquid aspiration mechanism is used to aspirate the reaction liquid after detection.

[0099] The ultrasonic device 10 is positioned close to the mixing position 102 and is used to perform ultrasonic mixing on the reaction liquid in the container 103 located on the mixing position 102.

[0100] In one embodiment, at least two mixing positions 102 are provided to increase the placement of the container cups 103. One ultrasonic device 10 corresponds to at least two mixing positions 102. That is, one ultrasonic device 10 can ultrasonically mix the reaction liquid 104 in multiple container cups 103 respectively. During the ultrasonic mixing process, the container cups 103 on other mixing positions 102 can be transferred or otherwise operated. The container cups 103 on multiple mixing positions 102 are alternately mixed to improve detection efficiency.

[0101] In one embodiment, the ultrasonic device 10 may also be configured in a one-to-one correspondence with the mixing position 102.

[0102] In one embodiment, the mixing position 102 may also be provided inside the reaction mechanism 40, and the ultrasonic device 10 is used to perform ultrasonic mixing operation on the reaction liquid in the container cup 103 inside the reaction mechanism 40.

[0103] Please refer to Figure 3 In one embodiment, a vortex mixing device 200 is also installed at the mixing position 102. The vortex mixing device 200 includes a drive motor 201, a transmission belt 202, an eccentric shaft 203, and a mounting base 204. The drive motor 201 is fixedly installed on the mounting base 204, with the output shaft of the drive motor 201 facing downwards. The eccentric shaft 203 is rotatably installed on the mounting base 204 through bearings. The eccentric shaft 203 is vertically arranged and has a first segment and a second segment that are not collinear. The first segment is located at the lower position, and the second segment is located at the upper position. Both the first segment and the second segment of the eccentric shaft 203 are parallel to the output shaft of the drive motor 201. Pullers are mounted on the output shaft of the drive motor 201 and the first section of the eccentric shaft 203, respectively. A transmission belt 202 connects the drive motor 201 and the pulleys of the eccentric shaft 203. The drive motor 201 drives the eccentric shaft 203 to rotate via the transmission belt 202. A cup holder 205 for placing the container cup 103 is mounted on the eccentric shaft 203, thereby causing the container cup 103 located on the cup holder 205 to rotate eccentrically, performing a vortex mixing operation on the reaction liquid inside the container cup 103. The drive motor 201 is connected to a controller 60, which controls the output power and output duration of the drive motor 201 to achieve various vortex ultrasonic modes of different intensities and durations.

[0104] In this embodiment, the controller 60 is connected to the ultrasonic device 10, the sample dispensing mechanism 22, the reagent dispensing mechanism 32, the reaction mechanism 40, the magnetic separation mechanism 50, the first transfer mechanism 81, and the second transfer mechanism 82, respectively. The controller 60 is used to control the test sequence of the entire sample analyzer.

[0105] When the controller 60 controls the ultrasound device 10, the controller 60 acquires the test items input or selected by the doctor, acquires the test item parameters corresponding to the test items, and matches one ultrasound mode from multiple ultrasound modes to perform ultrasound mixing operation on the reaction solution according to the test item parameters.

[0106] Different ultrasound modes have different ultrasound mixing intensities or different ultrasound mixing times. The mixing intensity is controlled by the input power and can be set to at least three ultrasound mixing intensities: strong, medium, and weak. It can also be set to at least two ultrasound mixing times: 1 second and 2 seconds.

[0107] Ultrasound modes include at least the following:

[0108] The first ultrasonic mode uses ultrasonic device 10 to perform ultrasonic mixing operation, with medium ultrasonic mixing intensity and 1 second ultrasonic mixing time.

[0109] The second ultrasonic mode uses ultrasonic device 10 to perform ultrasonic mixing operation, with weak ultrasonic mixing intensity and ultrasonic mixing time of 2s.

[0110] In the third ultrasonic mode, ultrasonic device 10 is used to perform ultrasonic mixing operation, the ultrasonic mixing intensity is strong, and the ultrasonic mixing time is 1 second.

[0111] Test parameters include numbers, letters, or a combination of both. For example, the test parameter for TNI (troponin) is 2, and the test parameters for E2 (estradiol) include 0 and 1. The controller 60 pre-stores test parameters corresponding to different test items, with each parameter corresponding to a specific ultrasound mode. For instance, test parameter 0 corresponds to the first ultrasound mode; when the controller 60 obtains a test parameter of 0, it drives the ultrasound device 10 to perform medium-intensity ultrasound mixing of the reaction solution for 1 second. Test parameter 1 corresponds to the second ultrasound mode; when the controller 60 obtains a test parameter of 1, it drives the ultrasound device 10 to perform weak-intensity ultrasound mixing of the reaction solution for 2 seconds. Test parameter 2 corresponds to the third ultrasound mode; when the controller 60 obtains test parameter 2, it drives the ultrasound device 10 to perform strong-intensity ultrasound mixing of the reaction solution for 1 second.

[0112] Different ultrasonic modes can be set according to specific test items so that the ultrasonic device 10 can effectively ultrasonically mix the reaction solution in different test items.

[0113] In this embodiment, the ultrasonic device 10 is positioned close to the mixing position 102. The ultrasonic device 10 can emit ultrasonic waves into the reaction liquid in the container cup 103 on the mixing position 102. The reaction liquid can be the reaction liquid before or after incubation, or it can be the reaction liquid into which the substrate is injected.

[0114] In one embodiment, the ultrasonic device 10 is positioned close to the sample application position 101, so that the ultrasonic device 10 can ultrasonically mix the sample in the container cup on the sample application position 101.

[0115] In one embodiment, the ultrasonic device 10 is positioned close to the reagent carrier 31, enabling the ultrasonic device 10 to ultrasonically mix the reagent within the reagent carrier 31.

[0116] Please refer to Figure 4In this embodiment, the ultrasound device 10 is an independent device, that is, the ultrasound device 10 can operate independently. For example, the ultrasound device 10 can operate independently of the sample dispensing mechanism 22, and the ultrasound device 10 can operate synchronously or asynchronously with other mechanisms to improve the efficiency of project testing.

[0117] The ultrasonic device 10 is a contact ultrasonic device. The ultrasonic device 10 includes an ultrasonic transducer 11, a transmission element 12, and a moving device 13. The ultrasonic transducer 11 includes a backing layer, a piezoelectric layer, and a matching layer connected in sequence. The piezoelectric layer is a piezoelectric crystal. Under the action of the driving electrical signal, the piezoelectric crystal generates compression and expansion in the thickness direction through the inverse piezoelectric effect. The frequency of this deformation reaches the ultrasonic frequency, forming ultrasonic vibration.

[0118] Please refer to Figure 4 and Figure 5 The transmission element 12 is a solid rod-shaped structure with a first end and a second end, the first end being the upper end and the second end being the lower end. The first end of the transmission element 12 has an external thread, and the lower end of the ultrasonic transducer 11 has an internal thread. The transmission element 12 is installed at the lower end of the ultrasonic transducer 11 by a threaded connection. The transmission element 12 can also be connected to the ultrasonic transducer 11 by snap-fit ​​or other methods. The transmission element 12 is a resonant rod and is connected to the matching layer of the ultrasonic transducer 11. The transmission element 12 is used to transmit ultrasonic vibrations. Compared with the hollow transmission element 12, the solid transmission element 12 is more conducive to the propagation of axial vibration. Furthermore, when the outer diameter of the transmission element 12 decreases along the direction of ultrasonic vibration transmission, the solid transmission element 12 is more conducive to energy convergence, thereby achieving a better ultrasonic mixing effect.

[0119] The outer diameter of the transmission element 12 gradually decreases or decreases in a stepwise manner from the first end to the second end. The transmission element 12 has the function of concentrating energy. When the ultrasonic vibration is transmitted from the first end to the second end, the axial cross-sectional area of ​​the second end is smaller than that of the first end. The ultrasonic vibration is more concentrated at the second end relative to the first end, which amplifies the amplitude of the emitted ultrasonic vibration at the second end of the transmission element 12 relative to the first end, thereby increasing the emitted ultrasonic energy.

[0120] Specifically, the transfer member 12 includes a first end 121, an intermediate section 122, and a second end 123. The first end 121 is a threaded connection end, and the second end 123 is a needle-shaped structure. The outer diameter of the second end 123 is smaller than the inner diameter of the receiving cup 103, allowing the second end 123 of the transfer member 12 to be inserted into the receiving cup 103. The intermediate section 122 has a trumpet-shaped structure. The end of the intermediate section 122 connected to the first end 121 is the large end of the trumpet, and the end of the intermediate section 122 connected to the second end 123 is the small end of the trumpet. The axial diameter of the intermediate section 122 gradually decreases from the large end to the small end of the trumpet.

[0121] The intermediate section 122 can also be composed of one or a conical rod, or any combination of both. Please refer to [reference needed]. Figure 6 The intermediate section 122 of structure a includes two cylindrical rods of different diameters; the intermediate section 122 of structure b includes four cylindrical rods of different diameters; the intermediate section 122 of structure c includes one conical rod; and the intermediate section 122 of structure d includes two cylindrical rods of different diameters and one conical rod. All five structures of the transmission component 12 described above are structures that gradually decrease in size or decrease in a stepped manner from the first end to the second end, which can amplify the amplitude.

[0122] Please refer to Figure 7 The mobile device 13 includes a mounting base 131, a swing arm assembly 132, a first moving assembly 133, and a second moving assembly 134.

[0123] The swing arm assembly 132 includes a swing arm 1321 and a lifting rod 1322. The lifting rod 1322 is vertically, liftably, and rotatably mounted on the mounting base 131. The swing arm 1321 is horizontally positioned, with one end connected to the lifting rod 1322. The ultrasonic transducer 11 is mounted on the end of the swing arm 1321 away from the lifting rod 1322. The swing arm assembly 132 is used to drive the ultrasonic transducer 11 and the transmission element 12 to move vertically and horizontally. In one embodiment, the swing arm 1321 and the lifting rod 1322 may also be an integrated structure.

[0124] The first moving component 133 is a lifting component, which includes a lifting motor 1331 and a lifting transmission component 1332. The lifting motor 1331 is mounted on the mounting base 131. The lifting transmission component 1332 includes a transmission wheel, a transmission belt, a gear, and a rack. The rack is vertically mounted on the lifting rod 1322, and the gear is rotatably mounted on the mounting base 131. The gear meshes with the rack. The lifting motor 1331 is connected to the gear through the transmission wheel and the transmission belt, and the lifting motor 1331 drives the lifting rod 1322 to move up and down through the gear and rack. In one embodiment, the first moving component 133 is a linear motor, and the output shaft of the linear motor is directly connected to the lifting rod 1322, which can also drive the lifting rod 1322 to move up and down.

[0125] The second moving component 134 is a rotating component, comprising a rotating motor 1341 and a rotating transmission component 1342. The rotating motor 1341 is mounted on the mounting base 131. The rotating transmission component 1342 includes a transmission belt and a rotating gear. The transmission belt is a gear belt. The rotating gear is fitted onto the lifting rod 1322 and is connected to the lifting rod 1322 via a key. The lifting rod 1322 can move up and down relative to the rotating gear, and the rotating gear drives the lifting rod 1322 to rotate. The rotating motor 1341 is connected to the rotating gear via the transmission belt and drives the lifting rod 1322 to rotate. In one embodiment, the rotating motor 1341 is connected to the lifting rod 1322 via a gear set, which also drives the lifting rod 1322 to rotate.

[0126] In one embodiment, the moving device 13 includes only a mounting base 131, a swing arm assembly 132, and a first moving assembly 133. The ultrasonic device 10 has a lifting function and is used to perform a mixing operation on the reaction liquid 104 in the container cup 103 on a specific mixing position 102.

[0127] In one embodiment, the second moving component 134 may also be a planar moving component composed of X-axis movement and Y-axis movement, with the X-axis movement and Y-axis movement respectively achieved by two motors, which can also drive the transmission component 12 to move alternately between multiple mixing positions 102.

[0128] Please refer to Figure 8 In this embodiment, the transmission element 12 of the ultrasonic device 10 is directly inserted into the reaction liquid 104 of the container 103. The ultrasonic device 10 has a preset frequency and voltage, so that the ultrasonic vibration mainly propagates along the axial direction. The second end face of the transmission element 12 is the emitting surface of the ultrasonic wave. During ultrasonic mixing, the second end face of the transmission element 12 emits ultrasonic waves into the reaction liquid 104, forming an ultrasonic sound field within the reaction liquid 104. Under the action of the ultrasonic sound field, the reaction liquid 104 will form a violent liquid flow to achieve the mixing of the components in the reaction liquid 104.

[0129] Besides the vibration effect of ultrasound, which enables the mixing of reaction liquid 104, the cavitation effect generated by ultrasound in the liquid can also uniformly disperse some aggregated and adhered substances in reaction liquid 104. When the frequency and sound pressure of the ultrasound are controlled, and combined with the amplification effect of the transmission element 12, the ultrasound energy entering the reaction liquid 104 in the container 103 is greater than the threshold of ultrasound cavitation, then ultrasound cavitation can occur in reaction liquid 104 during the ultrasound mixing process. When ultrasound cavitation occurs, it releases a large amount of energy, exerting a certain force on some aggregated and adhered substances in reaction liquid 104, causing them to disperse. At the same time, under the action of ultrasound vibration mixing, these substances can be uniformly dispersed in the reaction container.

[0130] In one embodiment, the ultrasonic device 10 is a non-contact ultrasonic mixing device. The ultrasonic device 10 is in contact with the container 103, and the ultrasonic waves emitted by the ultrasonic device 10 are transmitted to the reaction liquid inside the container 103 through the container 103.

[0131] Please refer to Figure 9 and Figure 10 The ultrasonic device 10 includes an ultrasonic transducer 11 and a transmission element 12. During ultrasonic mixing, the second end of the transmission element 12 rests against the outer wall of the receiving cup 103, transmitting ultrasonic vibrations to the reaction liquid 104 through the receiving cup 103. Since the transmission element 12 does not need to be inserted into the receiving cup 103, its axial length is shorter than that of a contact-type transmission element, but it also has the characteristic of gradually decreasing or stepwise decreasing from the first end to the second end to achieve amplified amplitude.

[0132] During ultrasonic mixing, the second end face of the transfer member 12 in this embodiment abuts against the outer wall of the container 103. The portion of the outer wall of the container 103 that contacts the transfer member 12 surrounds the reaction liquid 104, thereby transmitting the ultrasonic vibration generated by the ultrasonic transducer 11 to the liquid in the container 103. The portion of the container 103 that surrounds the reaction liquid 104 is the bottom of the container 103 and the lower sidewall connected to the bottom. Therefore, the ultrasonic vibration can be transmitted to the liquid in the container 103 at any position where the second end of the transfer member 12 abuts against the bottom of the container 103 and the lower sidewall connected to the bottom.

[0133] In this embodiment, the ultrasonic device 10 is a movable structure. The ultrasonic device 10 also includes a moving device, which includes a mounting base and a horizontal moving component. The horizontal moving component is mounted on the mounting base, and the ultrasonic transducer is mounted on the horizontal moving component. The horizontal moving component is a cylinder or a linear motor. The horizontal moving component is used to drive the second end of the transmission member 12 to abut against or move away from the outer wall of the receiving cup 103 on the mixing position 102.

[0134] In one embodiment, the ultrasonic device 10 is configured as a fixed structure, and the transmission member 12 is located in a preset position, such that after the receiving cup 103 is placed on the mixing position 102, the receiving cup 103 will directly contact the second end of the transmission member 12.

[0135] In this embodiment, the sample analyzer also includes a clamping device 110, which is used to limit the radial degree of freedom of the cup 103 on the mixing position 102.

[0136] Please refer to Figure 11 and Figure 12The clamping device 110 includes two clamping assemblies arranged opposite each other. Each clamping assembly includes a clamping motor 111, a clamping cam 112, and a clamping block 113. The clamping motor 111 is mounted on the base 100, with its output shaft facing upwards and vertically arranged. The output shaft of the clamping motor 111 is connected to the clamping cam 112, which is horizontally arranged and in contact with the clamping block 113. The clamping block 113 is horizontally movable and mounted on the base 100. The two sides of the clamping block 113 are adapted to the receiving cup 103 and the clamping cam 112, respectively. If the receiving cup 103 is a circular tube, the surface of the clamping block 113 facing the receiving cup 103 is a concave arc surface; if the receiving cup 103 is a square tube, the surface of the clamping block 113 facing the receiving cup 103 is a flat surface. The protrusion of the clamping cam 112 is a convex arc surface, while the clamping block 113 facing the clamping cam 112 is a concave arc surface with a larger curvature. This allows the concave arc surface of the clamping block 113 to guide the protrusion of the clamping cam 112 to slide in and out. The clamping motor 111 drives the clamping cam 112 to rotate, so that the clamping cam 112 drives the clamping block 113 to move closer to or away from the receiving cup 103. When the protrusions of both clamping cams 112 face the receiving cup 103 and are aligned on a line, the two clamping blocks 113 clamp the receiving cup 103, restricting the radial degree of freedom of the receiving cup 103. This prevents the receiving cup 103 from shaking during ultrasonic mixing and ensures good contact between the receiving cup 103 and the transfer member 12.

[0137] In this embodiment, since the ultrasonic device 10 contacts the lower end of the receiving cup 103 to achieve ultrasonic mixing, the clamping block 113 of the clamping device 110 clamps the lower side wall of the receiving cup 103 to improve the stability of the clamping. When the transmission member 12 of the ultrasonic device 10 abuts against the lower side wall of the receiving cup 103, the transmission member 12 and the clamping block 113 are staggered on the lower side wall of the receiving cup 103.

[0138] In one embodiment, the clamping device 110 may also include a linear drive and a clamping block. The linear drive is a cylinder or a linear motor. The linear drive drives the clamping block to move closer to and away from the receiving cup 103, which can also achieve the limiting of the receiving cup 103.

[0139] This embodiment uses a non-contact ultrasonic device 10, which can also transmit ultrasonic vibrations to the reaction liquid 104 in the container 103 to form an ultrasonic sound field and ultrasonic cavitation phenomenon, so as to ultrasonically mix the reaction liquid 104 in the container 103.

[0140] In one embodiment, a sample analysis method is provided, which is executed by the sample analyzer described in the above embodiment.

[0141] Please refer to Figure 13In the overall testing, depending on the different reagents used, the sample analyzer mainly includes the following five different testing procedures:

[0142] Test Procedure 1: One-Step Separation: After adding sample S and reagent R, perform one incubation and one magnetic separation operation, then add substrate A, incubate, and perform photometric measurement.

[0143] Test Procedure 2, Two-Step Separation in One Step: After adding sample S, the first step is to add reagent R1, which can be one or more reagents. The sample and reagent R1 are mixed to form a reaction solution for the first incubation. After the first incubation, the second step is to add reagent R2, which can be one or more reagents. Reagent R2 and the reaction solution after the first incubation form a new reaction solution for the second incubation. After the second incubation, the reaction solution is subjected to magnetic separation, substrate A addition, incubation, and photometric measurement in sequence.

[0144] Test Procedure 3: Two-Step Separation: After adding the sample, the first step is to add reagent R1. The sample and reagent R1 are mixed to form a reaction solution for the first incubation. After the first incubation, the first magnetic separation operation is performed. After the first magnetic separation operation, the second step is to add reagent R2. Reagent R2 and the reaction solution after the first magnetic separation form a new reaction solution for the second incubation. The reaction solution after the second incubation is used for the second magnetic separation operation. After the second magnetic separation operation, the reaction solution is used to add substrate A, incubate, and perform photometric measurements in sequence.

[0145] Test Procedure 4: Sample Pretreatment: Add sample S, then add pretreatment reagent to pretreat the sample to form sample S'; add reagent R to the pretreated sample S', and then perform incubation, magnetic separation, substrate A addition, incubation and photometry in sequence.

[0146] Test Procedure 5: Sample Pretreatment: Add sample S, then add diluent to dilute the sample to obtain a lower concentration sample S'; add reagent to the diluted sample S', and then perform incubation, magnetic separation, substrate A addition, incubation and photometry in sequence.

[0147] In this embodiment, the sample analysis method is controlled and executed by the controller 60, and is illustrated using a one-step magnetic separation method as an example. This sample analysis method involves ultrasonically mixing the incubated reaction solution.

[0148] Please refer to Figure 14 The sample analysis method in this embodiment includes the following steps:

[0149] S101: Sample injection;

[0150] The first transfer mechanism 81 transfers the new receiving cup 103 on the cup-loading mechanism 71 to the sample feeding position 101;

[0151] The sample dispensing mechanism 22 picks up the sample S from the sample carrying mechanism 21 and adds the picked-up sample S into the container cup 103 on the sample dispensing position 101.

[0152] S102: Add reagent;

[0153] The first transfer mechanism 81 transfers the container 103 containing sample S from the sample dispensing position 101 to the outer ring inside the reaction mechanism 40; the reaction mechanism 40 then transfers the container 103 to the reagent dispensing position;

[0154] The reagent dispensing mechanism 32 draws reagent R from the reagent carrying mechanism 31 and adds the drawn reagent to the container cup 103 at the sample reagent position in the reaction mechanism 40. The sample S and reagent R in the container cup 103 are mixed to form a reaction solution.

[0155] S103: Vortex mixing;

[0156] The second transfer mechanism 82 transfers the container 103 containing the reaction liquid to the mixing position 102;

[0157] A vortex mixer is used to vortex mix the reaction solution in container 103 to ensure that sample S and reagent R react fully.

[0158] S104: Incubation;

[0159] The second transfer mechanism 82 transfers the container 103 containing the vortex-mixed reaction liquid from the mixing position 102 back to the inner ring of the reaction mechanism 40 for incubation for a preset time.

[0160] S105: Ultrasonic mixing;

[0161] The second transfer mechanism 82 transfers the container 103 containing the incubated reaction solution from the reaction mechanism 40 to the mixing position 102.

[0162] The ultrasonic device 10 emits ultrasonic waves into the incubated reaction solution at the mixing station 102 to perform ultrasonic mixing. The ultrasonic device 10 uniformly disperses the aggregated substances in the reaction solution, thereby improving the accuracy of the test results.

[0163] The principle by which the controller 60 controls the ultrasonic device 10 to perform ultrasonic mixing is as follows:

[0164] The controller 60 acquires the test items input or selected by the doctor, acquires the test item parameters corresponding to the test items, and matches one ultrasound mode from multiple ultrasound modes according to the test item parameters to perform ultrasound mixing operation on the reaction solution. All ultrasound modes are executed by the ultrasound device 10.

[0165] Different ultrasound modes have different ultrasound mixing intensities or different ultrasound mixing times. The mixing intensity is controlled by the input power. Three ultrasound mixing intensities (strong, medium, weak) can be set, or more or fewer ultrasound mixing intensity gradients can be selected. Two ultrasound mixing times (1s and 2s) can be set.

[0166] Ultrasound modes include at least the following:

[0167] The first ultrasonic mode uses ultrasonic device 10 to perform ultrasonic mixing operation, with medium ultrasonic mixing intensity and 1 second ultrasonic mixing time.

[0168] The second ultrasonic mode uses ultrasonic device 10 to perform ultrasonic mixing operation, with weak ultrasonic mixing intensity and ultrasonic mixing time of 2s.

[0169] In the third ultrasonic mode, ultrasonic device 10 is used to perform ultrasonic mixing operation, the ultrasonic mixing intensity is strong, and the ultrasonic mixing time is 1 second.

[0170] Test parameters include numbers, letters, or a combination of both. For example, the test parameter for TNI (troponin) is 2, and the test parameters for E2 (estradiol) include 0 and 1. The controller 60 pre-stores test parameters corresponding to different test items. Each test parameter corresponds to an ultrasound mode. For example, test parameter 0 corresponds to the first ultrasound mode. When the controller 60 obtains test parameter 0, it drives the ultrasound device 10 to perform ultrasound mixing of the reaction solution at a medium intensity for 1 second. Test parameter 1 corresponds to the second ultrasound mode. When the controller 60 obtains test parameter 1, it drives the ultrasound device 10 to perform ultrasound mixing of the reaction solution at a weak intensity for 2 seconds. Test parameter 2 corresponds to the third ultrasound mode. When the controller 60 obtains test parameter 2, it drives the ultrasound device 10 to perform ultrasound mixing of the reaction solution at a strong intensity for 1 second.

[0171] Different ultrasonic modes can be set according to specific test items so that the ultrasonic device 10 can effectively ultrasonically mix the reaction solution in different test items.

[0172] S106: Magnetic separation;

[0173] The second transfer mechanism 82 transfers the container 103 containing the ultrasonically mixed reaction liquid from the mixing position 102 to the magnetic separation mechanism 50.

[0174] The cleaning fluid dispensing structure of the magnetic separation mechanism 50 dispenses cleaning fluid into the container cup 103 containing the reaction fluid;

[0175] The magnetic structure uses a magnetic field to attract the reaction complex containing magnetic beads inside the container cup 103.

[0176] The liquid-absorbing structure removes other substances and liquids, except for the adsorbed reaction complex, from the container cup 103.

[0177] S107: Add substrate;

[0178] The substrate dispensing mechanism of the magnetic separation unit 50 adds substrate A to the container 103 after liquid absorption. Substrate A luminescently marks the reaction complex in the reaction solution.

[0179] S108: Incubation;

[0180] The second transfer mechanism 82 transfers the container 103 containing the reaction solution injected with substrate A from the magnetic separation mechanism 50 back to the outer ring of the reaction mechanism 40 for incubation.

[0181] S109: Optical measurement;

[0182] The reaction mechanism 40 transfers the incubated reaction solution container 103 to the detection position;

[0183] The measuring apparatus 90 detects the luminescent reactive complex within the container cup 103;

[0184] After the photometry is completed, the waste liquid suction mechanism removes the reaction liquid from the container 103;

[0185] The first transfer mechanism 81 transfers the container 103 containing the discharged reaction liquid from the reaction mechanism 40 to the cup-throwing position 72.

[0186] This sample analysis method involves ultrasonic mixing of the incubated reaction solution (the reaction solution before magnetic separation). Ultrasonic mixing can uniformly disperse the aggregated substances in the incubated reaction solution, thereby improving the accuracy of detection.

[0187] To verify the effectiveness of this sample analysis method in improving detection accuracy, the following verification work was conducted:

[0188] Please refer to Figure 15 For the same batch of model samples, clinical tests were conducted using methods including and without ultrasonic mixing. The final test results were compared and compared with the standard test results of the centrifuged supernatant of the samples. The comparison of test results shows that after ultrasonic mixing, the test results of the model samples no longer closely approximate the true values ​​of the samples. That is, ultrasonic mixing of the reaction solution can improve the mixing effect, making the measured values ​​more accurate.

[0189] In one embodiment, ultrasonic mixing in step 105 is omitted, and step 103 is replaced with ultrasonic mixing. After ultrasonic mixing in step 105 is omitted, the second transfer mechanism 82 directly transfers the container 103 containing the incubated reaction solution into the magnetic separation mechanism 50 for magnetic separation operation.

[0190] In step 103, the ultrasonic device 10 is used to ultrasonically mix the reaction solution in the container 103 so that the sample S and reagent R can react fully.

[0191] In one embodiment, the ultrasonic mixing in step 105 is retained, and step 103 is replaced with ultrasonic mixing. In step 103, an ultrasonic device 10 is used to ultrasonically mix the reaction solution in the container 103 to ensure that the sample S and reagent R react fully.

[0192] Please refer to Figure 16 In the three embodiments described above, the reaction solution was subjected to ultrasonic treatment after incubation, the reaction solution before incubation was subjected to ultrasonic treatment, and the reaction solution before and after incubation was subjected to ultrasonic treatment, respectively. Compared with the traditional method that only mechanically mixes the reaction solution before incubation, the ultrasonic treatment in the above three embodiments has a better mixing effect and can improve the accuracy of the project detection.

[0193] In one embodiment, after adding the sample in step 101, an ultrasonic mixing step is added. Multiple ultrasonic devices 10 are provided, one of which is positioned close to the sample addition position 101. After sample addition is completed, the ultrasonic device 10 performs ultrasonic mixing on the sample in the receiving cup 103 at the sample addition position 101 to uniformly disperse the components in the sample. The ultrasonically mixed sample is then transferred to the reaction mechanism 40 for reagent addition.

[0194] In one embodiment, step 106 includes an ultrasonic mixing step, with multiple ultrasonic devices 10 provided, one of which is located near or inside the magnetic separation mechanism 50. The ultrasonic device 10 performs ultrasonic mixing on the reaction liquid containing the cleaning solution within the magnetic separation mechanism 50 to uniformly disperse the components in the reaction liquid, and then performs a magnetic attraction operation on the ultrasonically mixed reaction liquid containing the cleaning solution.

[0195] In one embodiment, an ultrasonic mixing step is added after step 107. The ultrasonic device 10 performs ultrasonic mixing on the reaction solution containing the substrate, located in the magnetic separation mechanism 50, on the mixing position 102, or in the reaction mechanism 40, so that the substrate and the reaction solution react fully. The ultrasonically mixed reaction solution containing the substrate is then incubated.

[0196] In one embodiment, an ultrasonic mixing step is added after step 108. The ultrasonic device 10 performs ultrasonic mixing on the incubated reaction liquid in the reaction mechanism 40 or on the mixing position 102. The incubated reaction liquid after ultrasonication is then subjected to photometry to improve the accuracy of the photometry.

[0197] In one embodiment, the sample dispensing position 101 is located within the reaction mechanism 40. In step 101, the first transfer mechanism 81 transfers the new container 103 from the upper cup mechanism 71 to the sample dispensing position 101 within the reaction mechanism 40, and the sample dispensing mechanism 22 dispenses the sample into the container 103 at the sample dispensing position 101 within the reaction mechanism 40, thus achieving sample dispensing as well.

[0198] In one embodiment, the mixing position 102 and the vortex mixing device are disposed within the reaction mechanism 40. In step 103, the reaction mechanism 40 rotates the container 103 containing the reaction liquid to the mixing position 102, or the reagent addition position and the mixing position 102 are located in the same position. After the reagent dispensing mechanism 32 adds the reagent, the vortex mixing device directly performs the vortex mixing operation. In step 105, the ultrasonic device 10 emits ultrasonic waves into the reaction liquid incubated at the mixing position 102 within the reaction mechanism 40 to perform ultrasonic mixing on the reaction liquid. Both vortex mixing and ultrasonic mixing operations can be achieved in this way.

[0199] In one embodiment, the mixing position 102 and the vortex mixing device are disposed within the magnetic separation mechanism 50. In step 103, the second transfer mechanism 82 transfers the container 103 containing the reaction liquid to the mixing position 102 within the magnetic separation mechanism 50; in step 105, the ultrasonic device 10 emits ultrasonic waves into the incubated reaction liquid at the mixing position 102 within the magnetic separation mechanism 50 to perform ultrasonic mixing. This also enables ultrasonic mixing.

[0200] In one embodiment, a second transfer mechanism 82 is used to throw the cup, and the cup-throwing position 72 is set within the stroke range of the second transfer mechanism 82. In step 109, the second transfer mechanism 82 transfers the container 103 containing the discharged reaction liquid from the reaction mechanism 40 to the cup-throwing position 72. This also enables the cup-throwing operation.

[0201] In one embodiment, a sample analysis method is provided. The difference between this sample analysis method and the above embodiment is that: after adding the sample, at least two reagents are added to the sample in sequence, and the reaction solution after adding the reagents is subjected to ultrasonic mixing.

[0202] Please refer to Figure 17 This embodiment of the sample analysis method uses the addition of three reagents as an example for illustration. The sample analysis method includes the following steps:

[0203] S201: Sample injection;

[0204] The first transfer mechanism 81 transfers the new receiving cup 103 on the cup-loading mechanism 71 to the sample feeding position 101;

[0205] The sample dispensing mechanism 22 picks up the sample from the sample carrying mechanism 21 and adds the picked-up sample S into the container cup 103 on the sample dispensing position 101.

[0206] S202: Add the first reagent;

[0207] The first transfer mechanism 81 transfers the container 103 containing the sample from the sample dispensing position 101 to the outer ring inside the reaction mechanism 40; the reaction mechanism 40 then transfers the container 103 containing the reagent R to the reagent dispensing position.

[0208] The reagent dispensing mechanism 32 draws the first reagent R1 from the reagent carrier mechanism 31 and adds the drawn first reagent R1 into the container cup 103 at the sample reagent position in the reaction mechanism 40. The sample S in the container cup 103 and the first reagent R1 are mixed to form a reaction solution.

[0209] S203: Ultrasonic mixing;

[0210] The second transfer mechanism 82 transfers the container 103 containing the reaction liquid to the mixing position 102;

[0211] The ultrasonic device is used to ultrasonically mix the reaction solution in the container 103 to ensure that the sample S and the first reagent R1 react fully.

[0212] The ultrasonic mixing operation is the same as in the above embodiments, but different ultrasonic modes are matched for different test items to effectively mix the reaction solution.

[0213] S204: Add the second reagent;

[0214] The second transfer mechanism 82 transfers the container 103 containing the reaction liquid from the mixing position 102 to the outer ring inside the reaction mechanism 40; the reaction mechanism 40 transfers the container 103 containing the reagent R to the reagent addition position.

[0215] The reagent dispensing mechanism 32 draws the second reagent R2 from the reagent carrier mechanism 31 and adds the drawn second reagent R2 into the container cup 103 at the sample reagent position in the reaction mechanism 40. The reaction solution in the container cup 103 and the second reagent R2 are mixed to form a reaction solution.

[0216] S205: Ultrasonic mixing;

[0217] The second transfer mechanism 82 transfers the container 103 containing the reaction liquid to the mixing position 102;

[0218] The ultrasonic device is used to ultrasonically mix the reaction solution in the container 103 to ensure that the sample and the second reagent R2 react fully.

[0219] The ultrasonic mixing operation is the same as in the above embodiments, but different ultrasonic modes are matched for different test items to effectively mix the reaction solution.

[0220] S206: Add a third reagent;

[0221] The second transfer mechanism 82 transfers the container 103 containing the reaction liquid from the mixing position 102 to the outer ring inside the reaction mechanism 40; the reaction mechanism 40 transfers the container 103 containing the reagent R to the reagent addition position.

[0222] The reagent dispensing mechanism 32 draws a third reagent R3 from the reagent carrier mechanism 31 and adds the drawn third reagent R3 into the container cup 103 at the sample reagent position in the reaction mechanism 40. The reaction solution in the container cup 103 and the third reagent R3 are mixed to form a reaction solution.

[0223] S207: Ultrasonic mixing;

[0224] The second transfer mechanism 82 transfers the container 103 containing the reaction liquid to the mixing position 102;

[0225] The ultrasonic device is used to ultrasonically mix the reaction solution in the container 103 to ensure that the sample and the third reagent R3 react fully.

[0226] The ultrasonic mixing operation is the same as in the above embodiments, but different ultrasonic modes are matched for different test items to effectively mix the reaction solution.

[0227] S208: Incubation;

[0228] The second transfer mechanism 82 transfers the container 103 containing the ultrasonically mixed reaction liquid from the mixing position 102 back to the inner ring of the reaction mechanism 40 for incubation for a preset time.

[0229] S209: Magnetic separation;

[0230] The second transfer mechanism 82 transfers the container 103 of the incubated reaction liquid from the reaction mechanism 40 to the magnetic separation mechanism 50;

[0231] The cleaning fluid dispensing structure of the magnetic separation mechanism 50 dispenses cleaning fluid into the container cup 103 containing the reaction fluid;

[0232] The magnetic structure uses a magnetic field to attract the reaction complex containing magnetic beads inside the container cup 103.

[0233] The liquid-absorbing structure removes other substances and liquids, except for the adsorbed reaction complex, from the container cup 103.

[0234] S210: Add substrate;

[0235] The substrate dispensing mechanism of the magnetic separation unit 50 adds substrate A to the container 103 after liquid absorption. Substrate A luminescently marks the reaction complex in the reaction solution.

[0236] S211: Incubation;

[0237] The second transfer mechanism 82 transfers the container 103 containing the reaction solution injected with substrate A from the magnetic separation mechanism 50 back to the outer ring of the reaction mechanism 40 for incubation.

[0238] S212: Optical measurement;

[0239] The reaction mechanism 40 transfers the incubated reaction solution container 103 to the detection position;

[0240] The measuring apparatus 90 detects the luminescent reactive complex within the container cup 103;

[0241] After the photometry is completed, the waste liquid suction mechanism removes the reaction liquid from the container 103;

[0242] The first transfer mechanism 81 transfers the container 103 containing the discharged reaction liquid from the reaction mechanism 40 to the cup-throwing position 72.

[0243] In the sample analysis method of this embodiment, after the three reagents are added in sequence, ultrasonic mixing is performed to ensure that the three reagents can react fully with the sample, thereby improving the accuracy of the detection.

[0244] In one embodiment, the ultrasonic device directly performs ultrasonic mixing of the reaction liquid within the reaction mechanism 40, without needing to transfer the container from the reaction mechanism 40 to the mixing position 102 for ultrasonic mixing.

[0245] Please refer to Figure 18 This embodiment is Example 1. After adding the sample, three reagents are added to the sample sequentially, and the reaction solution after each reagent addition is ultrasonically mixed. In some embodiments, the three reagents are added sequentially, and ultrasonic mixing is performed only on the reaction solution after adding one or two of the reagents. For example, in Examples 2 to 4, ultrasonic mixing is performed on the reaction solution after adding one reagent, and in Examples 5 to 7, ultrasonic mixing is performed on the reaction solution after adding any two reagents. All seven different ultrasonic mixing schemes can achieve the mixing effect.

[0246] In one embodiment, a sample analysis method is provided. The difference between this sample analysis method and the above embodiment is that: at least two reagents are added sequentially to a container, a sample is added to the container containing at least two reagents, and the reagents after adding the reagents are ultrasonically mixed.

[0247] Please refer to Figure 19 This embodiment of the sample analysis method uses the addition of three reagents as an example for illustration. The sample analysis method includes the following steps:

[0248] S301: Add the first reagent;

[0249] The first transfer mechanism 81 transfers the new container 103 from the upper container mechanism 71 to the reaction mechanism 40; the reaction mechanism 40 then transfers the container 103 to the reagent addition position.

[0250] The reagent dispensing mechanism 32 draws the first reagent R1 from the reagent carrier mechanism 31 and adds the drawn first reagent R1 into the container cup 103 at the sample reagent position in the reaction mechanism 40.

[0251] S302: Ultrasonic mixing;

[0252] The second transfer mechanism 82 transfers the container 103 containing the first reagent R1 to the mixing position 102;

[0253] The ultrasonic device is used to ultrasonically mix the reaction solution in the container 103 to ensure that the first reagent R1 is fully mixed.

[0254] The ultrasonic mixing operation is the same as in the above embodiments, but different ultrasonic modes are matched for different test items to effectively mix the first reagent R1.

[0255] S303: Add the second reagent;

[0256] The second transfer mechanism 82 transfers the container 103 containing the reaction liquid from the mixing position 102 to the outer ring inside the reaction mechanism 40; the reaction mechanism 40 transfers the container 103 containing the reagent R to the reagent addition position.

[0257] The reagent dispensing mechanism 32 draws the second reagent R2 from the reagent carrier mechanism 31 and adds the drawn second reagent R2 into the container cup 103 at the sample reagent position in the reaction mechanism 40. The first reagent R1 and the second reagent R2 in the container cup 103 form a mixed reagent.

[0258] S304: Ultrasonic mixing;

[0259] The second transfer mechanism 82 transfers the container 103 containing the first reagent R1 and the second reagent R2 to the mixing station 102.

[0260] The ultrasonic device is used to ultrasonically mix the reaction solution in the container 103 to ensure that the first reagent R1 and the second reagent R2 are fully mixed.

[0261] The ultrasonic mixing operation is the same as in the above embodiments, but different ultrasonic modes are matched for different test items to effectively mix the first reagent R1 and the second reagent R2.

[0262] S305: Add a third reagent;

[0263] The second transfer mechanism 82 transfers the container 103 containing the first reagent R1 and the second reagent R2 from the mixing position 102 to the outer ring inside the reaction mechanism 40; the reaction mechanism 40 then transfers the container 103 containing the reagent R to the reagent addition position.

[0264] The reagent dispensing mechanism 32 draws a third reagent R3 from the reagent carrying mechanism 31 and adds the drawn third reagent R3 into the container cup 103 at the sample reagent position in the reaction mechanism 40. The first reagent R1, the second reagent R2 and the third reagent R3 in the container cup 103 form a mixed reagent.

[0265] S306: Ultrasonic mixing;

[0266] The second transfer mechanism 82 transfers the container 103 containing the reaction liquid to the mixing position 102;

[0267] The ultrasonic device 10 mixes the reagents in the container 103 with ultrasound to ensure that the three reagents react fully.

[0268] The ultrasonic mixing procedure is the same as in the above embodiments, but different ultrasonic modes are matched for different test items to effectively mix the reagents.

[0269] S307: Sample injection;

[0270] The second transfer mechanism 82 transfers the container 103 containing the first reagent R1, the second reagent R2 and the third reagent R3 from the reaction mechanism 40 to the reaction mechanism 40, and the first transfer mechanism 81 transfers the container 103 containing the first reagent R1, the second reagent R2 and the third reagent R3 from the reaction mechanism 40 to the sample application position 101.

[0271] The sample dispensing mechanism 22 picks up the sample S from the sample carrying mechanism 21 and adds the picked-up sample S into the container cup 103 on the sample dispensing position 101, so that the first reagent R1, the second reagent R2, the third reagent R3 and the sample S are mixed to form a reaction solution.

[0272] S308: Incubation;

[0273] The first transfer mechanism 81 transfers the container 103 containing the ultrasonically mixed reaction solution from the sample application position 101 back to the inner ring of the reaction mechanism 40 for incubation for a preset time.

[0274] S309: Magnetic separation;

[0275] The second transfer mechanism 82 transfers the container 103 of the incubated reaction liquid from the reaction mechanism 40 to the magnetic separation mechanism 50;

[0276] The cleaning fluid dispensing structure of the magnetic separation mechanism 50 dispenses cleaning fluid into the container cup 103 containing the reaction fluid;

[0277] The magnetic structure uses a magnetic field to attract the reaction complex containing magnetic beads inside the container cup 103.

[0278] The liquid-absorbing structure removes other substances and liquids, except for the adsorbed reaction complex, from the container cup 103.

[0279] S310: Add substrate;

[0280] The substrate dispensing mechanism of the magnetic separation unit 50 adds substrate A to the container 103 after liquid absorption. Substrate A luminescently marks the reaction complex in the reaction solution.

[0281] S311: Incubation;

[0282] The second transfer mechanism 82 transfers the container 103 containing the reaction solution injected with substrate A from the magnetic separation mechanism 50 back to the outer ring of the reaction mechanism 40 for incubation.

[0283] S312: Optical measurement;

[0284] The reaction mechanism 40 transfers the incubated reaction solution container 103 to the detection position;

[0285] The measuring apparatus 90 detects the luminescent reactive complex within the container cup 103;

[0286] After the photometry is completed, the waste liquid suction mechanism removes the reaction liquid from the container 103;

[0287] The first transfer mechanism 81 transfers the container 103 containing the discharged reaction liquid from the reaction mechanism 40 to the cup-throwing position 72.

[0288] In the sample analysis method of this embodiment, after the three reagents are added in sequence, ultrasonic mixing is performed to ensure that the three reagents can react fully with the sample, thereby improving the accuracy of the detection.

[0289] In one embodiment, the ultrasonic device 10 performs ultrasonic mixing of the reaction liquid directly within the reaction mechanism 40, without needing to transfer the container from the reaction mechanism 40 to the mixing position 102 for ultrasonic mixing.

[0290] In one embodiment, the ultrasonic device 10 ultrasonically mixes the three reagents in the reagent carrying mechanism 21, and the reagent dispensing mechanism 22 then dispenses the ultrasonically mixed reagents into the receiving cup 103.

[0291] Please refer to Figure 20This embodiment is Example 1, in which three reagents are added sequentially, followed by the addition of a sample. Ultrasonic mixing is performed on the sample after each reagent addition. In some embodiments, three reagents are added sequentially, and ultrasonic mixing is performed only on the sample after adding one or two of the reagents. Examples 2 to 4 involve ultrasonic mixing of the sample after adding one reagent, while examples 5 to 7 involve ultrasonic mixing of the sample after adding any two reagents. All seven different ultrasonic mixing schemes can achieve the desired mixing effect.

[0292] In one embodiment, the sample analyzer is a biochemical analyzer, in which case the above sample analysis method does not involve magnetic separation of the reaction solution or addition of substrate.

[0293] The above specific examples illustrate the present invention only and are intended to aid in understanding the invention, not to limit it. Those skilled in the art can make various simple deductions, modifications, or substitutions based on the ideas of this invention.

Claims

1. A sample analyzer characterized by, The application relates to a sample testing device. The device comprises: a sample carrying mechanism for carrying a sample; a reagent carrying mechanism for carrying a reagent; a sample dispensing mechanism for sucking the sample from the sample carrying mechanism and discharging the sample into a containing cup; a reagent dispensing mechanism for sucking the reagent from the reagent carrying mechanism and discharging the reagent into the containing cup; a reaction mechanism for providing an incubation place for a reaction solution in the containing cup, the reaction solution being formed by mixing the sample and the reagent; a magnetic separation mechanism for performing a magnetic separation operation on the incubated reaction solution; a measuring mechanism for measuring the reaction solution; an ultrasonic device for generating ultrasonic vibration to form ultrasonic waves; and a controller connected with the ultrasonic device and the magnetic separation mechanism, the controller being used for controlling the ultrasonic device to emit ultrasonic waves into the incubated reaction solution before the magnetic separation operation, so as to disperse the agglomerated substances in the incubated reaction solution; and the controller is also used for controlling the magnetic separation mechanism to perform the magnetic separation operation on the incubated reaction solution which has been subjected to the ultrasonic wave emission by the ultrasonic device.

2. The sample analyzer of claim 1, wherein, The controller is used for controlling the ultrasonic device to emit ultrasonic waves into the incubated reaction solution in the next step after the incubation of the reaction solution is completed.

3. The sample analyzer of claim 1, wherein, The controller is used for acquiring a test item parameter, and matching an ultrasonic mode from a plurality of preset ultrasonic modes according to the test item parameter, so as to perform an ultrasonic mixing operation on the reaction solution.

4. The sample analyzer of claim 3, wherein, The plurality of ultrasonic modes respectively have different ultrasonic intensities and / or ultrasonic action times.

5. The sample analyzer of claim 1, wherein, The ultrasonic device comprises an ultrasonic transducer, a transmission member and a moving device, the ultrasonic transducer is used for forming ultrasonic vibration, the transmission member has a first end and a second end, the first end of the transmission member is connected with the ultrasonic transducer, and the outer diameter of the second end of the transmission member is smaller than the inner diameter of the containing cup; the moving device is connected with the ultrasonic transducer, and the moving device is used for driving the ultrasonic transducer and the transmission member to move relative to the containing cup, the second end of the transmission member can be inserted into the reaction solution in the containing cup, so as to emit ultrasonic waves into the reaction solution in the containing cup.

6. The sample analyzer of claim 1, wherein, The ultrasonic device comprises an ultrasonic transducer and a transmission member, the ultrasonic transducer is used for forming ultrasonic vibration, the transmission member has a first end and a second end, and the first end of the transmission member is connected with the ultrasonic transducer; the second end of the transmission member is used for abutting against the outer wall of the containing cup, and the part of the outer wall of the containing cup which is in contact with the transmission member is the part surrounding the reaction solution, so as to transmit the ultrasonic waves to the reaction solution in the containing cup.

7. The sample analyzer of claim 5 or 6, wherein The transmission member is of a solid structure, and the outer diameter of the transmission member gradually decreases or stepwisely decreases from the first end to the second end.

8. The sample analyzer of any one of claims 1 to 6, wherein, The ultrasonic device is used for performing an ultrasonic mixing operation on the incubated reaction solution in the reaction mechanism.

9. The sample analyzer of any one of claims 1 to 6, wherein, The device further comprises a transfer mechanism, the reaction mechanism is externally provided with a mixing position, and the transfer mechanism is used for transferring the containing cup containing the incubated reaction solution to the mixing position, and the ultrasonic device is used for performing an ultrasonic mixing operation on the incubated reaction solution located on the mixing position.

10. The sample analyzer of claim 1, wherein, The magnetic separation mechanism comprises a washing liquid dispensing structure, a magnetic separation structure, a liquid suction structure and a substrate dispensing structure. The washing liquid dispensing structure is used to inject washing liquid into the reaction liquid after incubation and ultrasonic mixing. The magnetic separation mechanism is used to perform magnetic separation operation on the reaction liquid after injection of washing liquid. The liquid suction structure is used to suck the liquid after magnetic separation. The substrate dispensing structure is used to inject substrate into the reaction liquid after magnetic separation.

11. The sample analyzer of claim 10, wherein, The ultrasonic device is also used to perform ultrasonic mixing operation on at least one of the sample, the reagent, the reaction liquid to be incubated, the reaction liquid after injection of washing liquid and the reaction liquid after injection of substrate.

12. A method of sample analysis, characterized by The method comprises the following steps: The sample dispensing mechanism and the reagent dispensing mechanism respectively inject sample and reagent into the containing cup to form reaction liquid; The reaction liquid is incubated in the reaction mechanism; The ultrasonic device emits ultrasonic waves to the reaction liquid after incubation; The transfer mechanism transfers the containing cup to the magnetic separation mechanism, and the magnetic separation mechanism performs magnetic separation operation on the reaction liquid after incubation and ultrasonic mixing; The substrate dispensing structure injects substrate into the reaction liquid after magnetic separation; The measuring mechanism measures the reaction liquid after injection of substrate.

13. The sample analysis method of claim 12, wherein, The ultrasonic mixing operation of the ultrasonic device is controlled by the following steps: According to the test item, the test item parameters are obtained; According to the test item parameters, one ultrasonic mode is matched from a plurality of preset ultrasonic modes to perform ultrasonic mixing operation on the reaction liquid.

14. The sample analysis method of claim 13, wherein, The plurality of ultrasonic modes respectively have different ultrasonic intensity and / or ultrasonic action time.

15. The sample analysis method as described in claim 12, characterized in that, The ultrasonic device emits ultrasonic waves to the reaction liquid after incubation in the reaction mechanism.

16. The sample analysis method as described in claim 12, characterized in that, Between the step of incubating the reaction liquid in the reaction mechanism and the step of the ultrasonic device emitting ultrasonic waves to the reaction liquid after incubation, the following steps are further included: The transfer mechanism transfers the containing cup containing the reaction liquid after incubation to the mixing position; The ultrasonic device emits ultrasonic waves to the reaction liquid after incubation on the mixing position.

17. The sample analysis method as described in claim 12, characterized in that, The step of the sample dispensing mechanism and the reagent dispensing mechanism respectively injecting sample and reagent into the containing cup to form reaction liquid comprises the following steps: The sample dispensing mechanism injects sample into the containing cup; The reagent dispensing mechanism injects reagent into the containing cup with sample to mix the sample and the reagent to form reaction liquid to be incubated.

18. The method of claim 12, wherein, The step of the sample dispensing mechanism and the reagent dispensing mechanism respectively injecting sample and reagent into the containing cup to form reaction liquid comprises the following steps: The reagent dispensing mechanism injects reagent into the containing cup; The sample dispensing mechanism injects sample into the containing cup with reagent to mix the sample and the reagent to form reaction liquid to be incubated.

19. The sample analysis method of claim 17 or 18, wherein, Between the step of the sample dispensing mechanism and the reagent dispensing mechanism respectively injecting sample and reagent into the containing cup to form reaction liquid and the step of incubating the reaction liquid in the reaction mechanism, the following steps are further included: The transfer mechanism transfers the containing cup containing the reaction liquid to be incubated to the mixing position; The ultrasonic device emits ultrasonic waves to the reaction liquid to be incubated on the mixing position; The transfer mechanism transfers the reaction liquid after ultrasonic mixing to the reaction mechanism.

20. The sample analysis method of claim 17 or 18, wherein, Before the step of the sample dispensing mechanism and the reagent dispensing mechanism respectively injecting sample and reagent into the holding cup to form a reaction solution, the ultrasonic device emits ultrasonic waves to the reagent in the reagent carrying mechanism.

21. The sample analysis method of claim 17 or 18, wherein, Before the step of the reagent dispensing mechanism injecting reagent into the holding cup, the ultrasonic device emits ultrasonic waves to the reagent in the reagent carrying mechanism.

22. The sample analysis method as described in claim 18, characterized in that, Between the step of the reagent dispensing mechanism injecting reagent into the holding cup and the step of the sample dispensing mechanism injecting sample into the holding cup with reagent, the ultrasonic device emits ultrasonic waves to the reagent in the holding cup.

23. The sample analysis method of claim 17 or 18, wherein, The reagent dispensing mechanism injects at least two reagents into the holding cup in multiple steps; After at least one of the multiple steps of the reagent dispensing mechanism, the ultrasonic device emits ultrasonic waves to the reaction solution or reagent in the holding cup.

24. The sample analysis method of claim 12, wherein, Between the step of the ultrasonic device emitting ultrasonic waves to the incubated reaction solution and the step of the measuring mechanism performing optical measurement on the reaction solution, the ultrasonic device emits ultrasonic waves to the reaction solution in the holding cup. The substrate dispensing mechanism injects substrate into the reaction solution after magnetic separation; The transfer mechanism transfers the reaction solution with injected substrate back to the reaction mechanism, and the reaction solution with injected substrate is incubated in the reaction mechanism.

25. The sample analysis method of claim 24, wherein, Before the reaction solution with injected substrate is incubated in the reaction mechanism, the ultrasonic device emits ultrasonic waves to the reaction solution with injected substrate in the holding cup.

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