An embedded dielectrophoresis in-situ Raman spectroscopy system

Abstract

An embedded dielectrophoresis in-situ Raman spectroscopy system, a first laser emitter emits a first laser to a first lens group, the first laser is converged by the first lens group and incident to a designated area of ​​a sample container flow channel; the first laser emitted from the sample container enters an imaging camera through an objective lens; a second laser emitter emits a second laser to a second lens group, the second laser is incident to a designated area of ​​a sample container flow channel through an objective lens after changing the optical path by selecting a lens group, the liquid bubble or the droplet surface in a microemulsion controlled by dielectrophoresis in the designated area reflects the second laser, the second laser reflected by the droplet surface of the liquid bubble changes the optical path by selecting a lens group and is incident to a third lens group, the second laser emitted by the third lens group is captured by a Raman spectrometer. The present invention can dynamically display the surface properties of liquid bubbles or droplets in microemulsions in real time, and can provide a stable, controllable, non-contact, and non-destructive in-situ characterization method for dynamic analysis of liquids such as water or microemulsions at a micro-mesoscopic scale.

Description

Technical Field

[0001] The invention belongs to the technical field of droplet forming, and in particular relates to an embedded dielectrophoresis in-situ Raman spectroscopy system. Background Art

[0002] Since the discovery of the microemulsion system in 1943, theoretical research has made great progress and it has been widely used in food, cosmetics, agriculture, pharmaceuticals, medical and other fields. Characterization is a necessary means of material research. There are many means to characterize the morphology and physical properties of microemulsions, such as electron microscopy, scattering technology, spectroscopy technology, etc. However, these characterization methods have their inherent defects and single functions.

[0003] Electron microscopy technology requires freezing the microemulsion for sample preparation, which will damage the structure of the microemulsion and make dynamic observation impossible. Scattering technology and spectroscopy technology have been successfully applied, but the related equipment is relatively complex and the measurement process takes a long time. Temperature, as an important consideration for emulsions, plays an important role in the preparation of microemulsions and subsequent changes. Scattering technology and spectroscopy technology cannot perform in-situ and instant characterization of the morphology of droplets in microemulsions during temperature changes. Most of the existing technologies are based on the speculation of mechanisms based on phenomena. The real-time display of droplet morphology and the acquisition of interface information are technical problems that need to be solved in the development of microemulsion technology. Summary of the invention

[0004] To this end, the present invention provides an embedded dielectrophoresis in-situ Raman spectroscopy system to solve the problem that the prior art cannot effectively display the droplet morphology in real time and obtain interface information.

[0005] In order to achieve the above-mentioned object, the present invention provides the following technical solutions: an embedded dielectrophoresis in-situ Raman spectroscopy system, comprising a first laser emitter, a first lens group, a sample container, an objective lens, an imaging camera, a second laser emitter, a second lens group, a selection lens group, a third lens group and a Raman spectrometer;

[0006] The first laser emitter emits a first laser toward the first lens group, and the first laser emitted by the first laser emitter is converged by the first lens group and incident on a designated area of ​​the flow channel of the sample container; the first laser emitted from the sample container passes through the objective lens and enters the imaging camera;

[0007] The second laser emitter emits a second laser toward the second lens group. The second laser emitted by the second laser emitter is incident on a designated area of ​​the sample container flow channel through the objective lens after the optical path is changed by the selection lens group. The liquid bubbles or droplet surfaces in the microemulsion in the designated area of ​​the sample container flow channel reflect the second laser. The second laser reflected by the droplet surfaces of the liquid bubbles changes its optical path through the selection lens group and is incident on the third lens group. The second laser emitted by the third lens group is incident on the Raman spectrometer.

[0008] As a preferred solution of the embedded dielectrophoresis in-situ Raman spectroscopy system, a first aperture is provided between the first lens group and the sample container, and the first aperture selects the range and position of the incident area of ​​the first laser in the sample container;

[0009] A second aperture is provided between the objective lens and the sample container, and the second aperture selects the range and position of the incident area of ​​the second laser in the sample container;

[0010] The selection lens group is two selection lenses stacked in the radial direction of the microscope, and the selection lens group is used to provide a new optical path channel for microscope imaging so as to be incident from the top of the sample.

[0011] As a preferred solution for the embedded dielectrophoresis in-situ Raman spectroscopy system, it also includes a display terminal. The imaging camera adopts an ultra-high-speed camera with a CCD photosensitive element, and the imaging camera and the display terminal are connected; the display terminal is a PC that can process and display image data, and the display terminal is used to dynamically display the surface morphology of liquid bubbles or droplets in the microemulsion in a specified area in the flow channel of the sample container.

[0012] As a preferred solution of the embedded dielectrophoresis in-situ Raman spectroscopy system, the display terminal is also connected to the Raman spectrometer, and the display terminal jointly displays the droplet surface morphology, Raman spectroscopy signals and processed indicators.

[0013] As a preferred solution for an embedded dielectrophoresis in-situ Raman spectroscopy system, the sample container is integrated with a fluid channel, dielectrophoresis electrodes are deposited on both sides of the fluid channel, the dielectrophoresis electrodes are connected to a signal generator, and the dielectrophoresis electrodes are used to control the spatial position of bubbles in a liquid or droplets in a microemulsion within a specified area.

[0014] As a preferred solution for the embedded dielectrophoresis in-situ Raman spectroscopy system, a base is arranged below the sample container, and the sample container is made of ITO, SiC, IZO, Al 2 O 3 and one of the organic compounds.

[0015] As a preferred solution for the embedded dielectrophoresis in-situ Raman spectroscopy system, the dielectrophoresis electrode material is at least one of metal Au, Ag, Sb, Sn, Pt, Cu, Al, or semiconductor IZO, ITO.

[0016] As a preferred solution of the embedded dielectrophoresis in-situ Raman spectroscopy system, the material of the first aperture and the second aperture is at least one of Cu, Al, Mo, and Pt.

[0017] As a preferred solution of the embedded dielectrophoresis in-situ Raman spectroscopy system, the first laser emitted by the first laser emitter is any one of red light, blue light and green light;

[0018] The second laser wavelength emitted by the second laser emitter is any one of 785nm / 1300nm.

[0019] As a preferred solution of the embedded dielectrophoresis in-situ Raman spectroscopy system, the wavelength of the first laser emitted by the first laser emitter is green light of 525 nm;

[0020] The wavelength of the second laser emitted by the second laser emitter is 785 nm.

[0021] The present invention has the following advantages: a first laser emitter, a first lens group, a sample container, an objective lens, an imaging camera, a second laser emitter, a second lens group, a selection lens group, a third lens group and a Raman spectrometer are provided; the first laser emitter emits a first laser to the first lens group, and the first laser emitted by the first laser emitter is incident on a designated area of ​​the sample container flow channel after being converged by the first lens group; the first laser emitted from the sample container enters the imaging camera through the objective lens; the second laser emitter emits a second laser to the second lens group, and the second laser emitted by the second laser emitter is incident on a designated area of ​​the sample container flow channel through the objective lens after the optical path is changed by the selection lens group, and the liquid bubble or the droplet surface in the microemulsion in the designated area of ​​the sample container flow channel reflects the second laser, and the second laser reflected by the droplet surface of the liquid bubble changes the optical path by the selection lens group and is incident on the third lens group, and the second laser emitted by the third lens group is incident on the Raman spectrometer. The present invention can dynamically display the surface properties of liquid bubbles or droplets in microemulsions in real time, and can provide a stable, controllable, non-contact, non-destructive in-situ characterization method for dynamic analysis of liquids such as water or microemulsions at a micro-mesoscopic scale. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0023] The structures, proportions, sizes, etc. illustrated in this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with the technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantial technical significance. Any structural modification, change in proportion or adjustment of size shall still fall within the scope of the technical contents disclosed in the present invention without affecting the effects and purposes that can be achieved by the present invention.

[0024] Figure 1 Schematic diagram of an embedded dielectrophoresis in-situ Raman spectroscopy system provided in an embodiment of the present invention;

[0025] Figure 2 This is an example of modification of the embedded dielectrophoresis in-situ Raman spectroscopy system provided in an embodiment of the present invention.

[0026] In the figure, 1. first laser emitter; 2. first lens group; 3. sample container; 4. objective lens; 5. imaging camera; 6. second laser emitter; 7. second lens group; 8. selection lens group; 9. third lens group; 10. Raman spectrometer; 11. first aperture; 12. second aperture; 13. display terminal; 14. fluid channel; 15. dielectrophoresis electrode; 16. base. DETAILED DESCRIPTION

[0027] The following is a description of the implementation of the present invention by specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] See also Figure 1 and Figure 2 , an embodiment of the present invention provides an embedded dielectrophoresis in-situ Raman spectroscopy system, comprising a first laser emitter 1, a first lens group 2, a sample container 3, an objective lens 4, an imaging camera 5, a second laser emitter 6, a second lens group 7, a selection lens group 8, a third lens group 9 and a Raman spectrometer 10;

[0029] The first laser emitter 1 emits a first laser to the first lens group 2. The first laser emitted by the first laser emitter 1 is converged by the first lens group 2 and incident on a designated area of ​​the flow channel of the sample container 3. The first laser emitted from the sample container 3 passes through the objective lens 4 and enters the imaging camera 5.

[0030] The second laser emitter 6 emits a second laser toward the second lens group 7. The second laser emitted by the second laser emitter 6 is incident on a designated area of ​​the flow channel of the sample container 3 through the objective lens 4 after the optical path is changed by the selection lens group 8. The liquid bubbles or droplet surfaces in the microemulsion in the designated area of ​​the flow channel of the sample container 3 reflect the second laser. The second laser reflected by the droplet surfaces of the liquid bubbles is changed in optical path by the selection lens group 8 and is incident on the third lens group 9. The second laser emitted by the third lens group 9 is incident on the Raman spectrometer 10.

[0031] In this embodiment, the first lens group 2 includes a plurality of biconvex mirrors, the second lens group 7 includes two biconvex mirrors and a reflector, and the emission directions of the first laser and the second laser are perpendicular. The selection lens group 8 is two selection lenses stacked in the radial direction of the microscope, which can provide a new optical path channel for microscope imaging so that it can be incident from the top of the sample.

[0032] In this embodiment, a first aperture 11 is provided between the first lens group 2 and the sample container 3, and the first aperture 11 selects the range and position of the incident area of ​​the first laser in the sample container 3; a second aperture 12 is provided between the objective lens 4 and the sample container 3, and the second aperture 12 selects the range and position of the incident area of ​​the second laser in the sample container 3. The materials of the first aperture 11 and the second aperture 12 include but are not limited to materials with low light transmittance such as Cu, Al, Mo, and Pt. The first laser emitted by the first laser emitter 1 is any one of red light, blue light, and green light; the wavelength of the second laser emitted by the second laser emitter 6 is any one of 785nm / 1300nm. The wavelength of the first laser emitted by the first laser emitter 1 is green light, and the optimal wavelength is 525nm; the wavelength of the second laser emitted by the second laser emitter 6 is optimally 785nm.

[0033] Specifically, the first aperture 11 can make the first laser incident on the selected area of ​​the sample container 3, and the light path passing through the first aperture 11 then passes through the objective lens 4 to enter the imaging camera 5, and the operating area can be directionally selected with the help of the imaging camera 5. The second aperture 12 is close to the top of the sample container 3, and the second aperture 12 facilitates the positioning of the spectral system. The second laser passes through the second aperture 12 and is reflected on the bubble in the liquid in the specified area, that is, the gas-liquid surface or the surface of the small droplets in the microemulsion, and then changes the light path through the selection lens group 8, is emitted to the third lens group 9, and then is emitted into the Raman spectrometer 10.

[0034] In this embodiment, a display terminal 13 is also included. The imaging camera 5 uses an ultra-high-speed camera with a CCD photosensitive element. The imaging camera 5 and the display terminal 13 are connected. The display terminal 13 is a PC that can process and display image data. The display terminal 13 is used to dynamically display the surface morphology of liquid bubbles or droplets in the microemulsion in the specified area of ​​the flow channel of the sample container 3. The display terminal 13 is also connected to the Raman spectrometer 10. The display terminal 13 jointly displays the surface morphology of the droplets, the Raman spectrum signal and the processed indicators. The sample container 3 is integrated with a fluid channel 14. Dielectrophoresis electrodes 15 are deposited on both sides of the fluid channel 14. The material of the dielectrophoresis electrode 15 is at least one of metal Au, Ag, Sb, Sn, Pt, Cu, Al, or semiconductor IZO, ITO. The dielectrophoresis electrode 15 is connected to a signal generator. The dielectrophoresis electrode 15 is used to control the spatial position of bubbles in the liquid or droplets in the microemulsion in the specified area.

[0035] Specifically, the imaging camera 5 and the Raman spectrometer 10 are both prior art, and are connected to a display terminal 13 with a display, which can be used to select the operation area of ​​the sample container 3. The fluid channel 14 allows the liquid to flow, and the dielectrophoresis electrode 15 controls the movement of bubbles in the liquid or small droplets in the microemulsion in the area, and can also fix the bubbles in the liquid or small droplets in the microemulsion in the control area, which is convenient for observation. With the help of the display terminal 13, the properties of the object to be tested can be dynamically displayed in real time, providing an effective in-situ characterization method for the dynamic analysis of microemulsions at the micro-mesoscopic scale.

[0036] In this embodiment, a base 16 is disposed below the sample container 3, and the sample container 3 is made of materials including but not limited to materials with high light transmittance such as ITO, SiC, IZO, Al2O3, and SiO2. That is, the sample container 3 and the base 16 are both made of transparent materials, which facilitates observation of the sample container 3 from a macroscopic perspective, and the base 16 does not affect the passage of the laser while playing a supporting role.

[0037] In summary, the present invention is provided with a first laser emitter 1, a first lens group 2, a sample container 3, an objective lens 4, an imaging camera 5, a second laser emitter 6, a second lens group 7, a selection lens group 8, a third lens group 9 and a Raman spectrometer 10; the first laser emitter 1 emits a first laser to the first lens group 2, and the first laser emitted by the first laser emitter 1 is incident on a designated area of ​​a flow channel of the sample container 3 after being converged by the first lens group 2; the first laser emitted from the sample container 3 enters the imaging camera 5 through the objective lens 4; the second laser emitter 6 emits a second laser to the second lens group 7, and the second laser emitted by the second laser emitter 6 is incident on a designated area of ​​a flow channel of the sample container 3 through the objective lens 4 after the optical path is changed by the selection lens group 8, the liquid bubble or the droplet surface in the microemulsion in the designated area of ​​the flow channel of the sample container 3 reflects the second laser, the second laser reflected by the droplet surface of the liquid bubble changes its optical path through the selection lens group 8 and is incident on the third lens group 9, and the second laser emitted by the third lens group 9 is incident on the Raman spectrometer 10. The sample container 3 is integrated with a fluid channel 14, and a dielectrophoresis electrode 15 set according to a specific function is integrated near the fluid channel 14. The dielectrophoresis electrode 15 is connected to a signal generator and is used to control the spatial position of bubbles in the liquid or droplets in the microemulsion in the specified area. The first aperture 11 enables the first laser to be incident on the selected area of ​​the sample container 3. The light path passing through the first aperture 11 then passes through the objective lens 4 and enters the imaging camera 5. With the help of the imaging camera 5, the operating area can be directional selected. The second aperture 12 is close to the top of the sample container 3. The second aperture 12 facilitates the positioning of the spectral system. The second laser passes through the second aperture 12 and is reflected on the bubble in the liquid in the specified area, that is, the gas-liquid surface or the surface of the small droplets in the microemulsion, and then changes the light path through the selection lens group 8, and is emitted to the third lens group 9, and then enters the Raman spectrometer 10. The fluid channel 14 of the present invention allows liquid to flow, and the dielectrophoresis electrode 15 can control the movement of bubbles in the liquid or small droplets in the microemulsion in the area, and can also fix the bubbles in the liquid or small droplets in the microemulsion in the control area, which is convenient for observation. With the help of the display terminal 13, the properties of the object to be tested can be displayed dynamically in real time, providing an effective in-situ characterization method for the dynamic analysis of microemulsions at the micro-mesoscopic scale. The present invention can dynamically display the surface properties of liquid bubbles or droplets in microemulsions in real time, and can provide a stable, controllable, non-contact, and non-destructive in-situ characterization method for the dynamic analysis of liquids such as water or microemulsions at the micro-mesoscopic scale.

[0038] Although the present invention has been described in detail above by general description and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements can be made to the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. An embedded dielectrophoresis in-situ Raman spectroscopy system, characterized in that: It comprises a first laser emitter (1), a first lens group (2), a sample container (3), an objective lens (4), an imaging camera (5), a second laser emitter (6), a second lens group (7), a selection lens group (8), a third lens group (9) and a Raman spectrometer (10); The first laser emitter (1) emits a first laser toward the first lens group (2); the first laser emitted by the first laser emitter (1) is converged by the first lens group (2) and then incident on a designated area of ​​the flow channel of the sample container (3); the first laser emitted from the sample container (3) passes through the objective lens (4) and enters the imaging camera (5); The second laser emitter (6) emits a second laser toward the second lens group (7); the second laser emitted by the second laser emitter (6) is incident on a designated area of ​​the flow channel of the sample container (3) through the objective lens (4) after the optical path of the second laser is changed by the selection lens group (8); the second laser is reflected by the surface of the liquid bubble or the liquid droplet in the microemulsion in the designated area of ​​the flow channel of the sample container (3); the second laser reflected by the liquid droplet surface of the liquid bubble changes its optical path through the selection lens group (8) and is incident on the third lens group (9); the second laser emitted by the third lens group (9) is incident on the Raman spectrometer (10); A first aperture (11) is provided between the first lens group (2) and the sample container (3), and the first aperture (11) selects the range and position of the incident area of ​​the first laser on the sample container (3); A second aperture (12) is provided between the objective lens (4) and the sample container (3), and the second aperture (12) selects the range and position of the incident area of ​​the second laser light in the sample container (3); The selection lens group (8) is two selection lenses stacked in the radial direction of the microscope, and the selection lens group (8) is used to provide a new light path channel for microscope imaging so as to be incident from the top of the sample; It also includes a display terminal (13), wherein the imaging camera (5) is an ultra-high-speed camera with a CCD light-sensing element, and the imaging camera (5) and the display terminal (13) are connected; the display terminal (13) is a PC for processing image data and displaying the image data; the display terminal (13) is used to dynamically display the surface morphology of liquid bubbles or droplets in the microemulsion in a specified area of ​​the flow channel of the sample container (3); The sample container (3) is integrated with a fluid channel (14), and dielectrophoresis electrodes (15) are deposited on both sides of the fluid channel (14). The dielectrophoresis electrodes (15) are connected to a signal generator, and the dielectrophoresis electrodes (15) are used to control the spatial position of bubbles in a liquid or droplets in a microemulsion within a specified area.

2. The embedded dielectrophoresis in-situ Raman spectroscopy system according to claim 1, characterized in that: The display terminal (13) is also connected to the Raman spectrometer (10), and the display terminal (13) jointly displays the droplet surface morphology, the Raman spectrum signal, and the index obtained by processing.

3. The embedded dielectrophoresis in-situ Raman spectroscopy system according to claim 1, characterized in that: A base (16) is arranged below the sample container (3), and the sample container (3) is made of a material selected from the group consisting of ITO, SiC, IZO, Al2O3 and an organic compound.

4. The embedded dielectrophoresis in-situ Raman spectroscopy system according to claim 3, characterized in that: The dielectrophoresis electrode (15) is made of at least one of metal Au, Ag, Sb, Sn, Pt, Cu, Al, or semiconductor IZO and ITO.

5. The embedded dielectrophoresis in-situ Raman spectroscopy system according to claim 1, characterized in that: The material of the first aperture (11) and the second aperture (12) is at least one of Cu, Al, Mo, and Pt.

6. The embedded dielectrophoresis in-situ Raman spectroscopy system according to claim 1, characterized in that: The first laser emitted by the first laser emitter (1) is any one of red light, blue light and green light; The wavelength of the second laser emitted by the second laser emitter (6) is any one of 785nm / 1300nm.

7. The embedded dielectrophoresis in-situ Raman spectroscopy system according to claim 6, characterized in that: The wavelength of the first laser emitted by the first laser emitter (1) is green light of 525 nm; the wavelength of the second laser emitted by the second laser emitter (6) is 785 nm.

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