A dual-channel spectral detection device for capillary electrophoresis

Abstract

The application relates to the field of electrophoretic detection, and particularly discloses a double-channel spectral detection device for capillary electrophoresis, which comprises an electrophoretic capillary, a first detection mechanism and a second detection mechanism are arranged on the outer side of the electrophoretic capillary, the first detection mechanism and the second detection mechanism are arranged perpendicularly to each other on the outer side of the electrophoretic capillary, and a second light condensing sheet is connected to one end of the first detection mechanism and the second detection mechanism close to the electrophoretic capillary. The sensitivity of the device is improved by using a spectral analyzer and a laser for detecting trace samples and trace samples, the experimental reliability is improved, the detection target is expanded, the working efficiency of the device is improved, the fluorescence cross is reduced, the experimental accuracy is improved, the manufacturing cost of a dye reagent box is reduced, the detection items are increased, and the accuracy of detection can be conveniently controlled through optical fiber transmission of laser and fluorescence signals to the capillary and the spectral analyzer.

Description

Technical Field

[0001] This invention relates to the field of electrophoresis detection, specifically a dual-channel spectral detection device for capillary electrophoresis. Background Technology

[0002] The current international standard technology uses a single laser, a single detector, and multiple fluorescent capillary gene detection method, which is currently the gold standard in clinical medicine, judicial organs, agricultural research, and other fields. Because it uses a single laser to excite multiple fluorescent dyes, this method can only excite a maximum of six different fluorescent dyes, and the maximum number of sites that can be excited is 24. However, most current testing projects require the excitation of 60-100 sites, so the existing technology is no longer sufficient for market applications. Summary of the Invention

[0003] To address the existing problems, this invention provides a dual-channel spectral detection device for capillary electrophoresis, which, when used in conjunction with other devices, can effectively solve the problems mentioned in the background art.

[0004] To solve the above problems, the present invention adopts the following technical solution:

[0005] A dual-channel spectral detection device for capillary electrophoresis includes an electrophoretic capillary. A first detection mechanism and a second detection mechanism are disposed on the outer side of the electrophoretic capillary. The first detection mechanism and the second detection mechanism are arranged perpendicularly to each other on the outer side of the electrophoretic capillary. A second focusing sheet is connected to one end of the first detection mechanism and the second detection mechanism near the end of the electrophoretic capillary.

[0006] As a further embodiment of the present invention: a dichroic mirror is provided on one side of the second condenser inside the second detection mechanism, a reflector is provided directly below the dichroic mirror, a cutoff filter is installed on the side of the dichroic mirror away from the second condenser, and a filter is provided directly below the cutoff filter.

[0007] As a further embodiment of the present invention: a spectrometer is provided on one side of the cutoff filter, a first focusing plate is provided between the cutoff filter and the spectrometer, and a laser is provided on the side of the filter away from the reflector.

[0008] As a further embodiment of the present invention: the first detection mechanism and the second detection mechanism have the same structure, and at least one set of the first detection mechanism and the second detection mechanism is provided on the outside of the electrophoresis capillary.

[0009] As a further embodiment of the present invention: the dichroic mirror and the reflector are inclined inside the second detection mechanism, and the inclination angles are equal.

[0010] As a further embodiment of the present invention: the axial position of the spectrometer coincides with the center position of the first focusing plate, and the axial position of the laser coincides with the center position of the filter.

[0011] Compared with the prior art, the beneficial effects of the present invention are:

[0012] 1. The use of spectrometers and lasers improves the sensitivity and reliability of the detection of trace and micro-samples; expands the detection targets and improves the efficiency of the instrument; reduces fluorescence cross-linking and improves the accuracy of the experiment; reduces the manufacturing cost of dye reagent kits and increases the number of detection items. The laser and fluorescence signals are transmitted to the capillary and spectrometer through optical fiber, which can facilitate the control of the accuracy of the detection.

[0013] 2. At least one set of first and second detection mechanisms set on the outside of the electrophoresis capillary can realize the collection of fluorescence at different wavelengths, and the whole device adopts a closed optical fiber transmission method, which solves the problem that the existing instruments cannot be portable due to the combined reflection of the mirror. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of a dual-channel spectral detection device for capillary electrophoresis.

[0015] Figure 2 This is a schematic diagram of the optical path transmission in a dual-channel spectral detection device for capillary electrophoresis.

[0016] In the diagram: 1. First testing unit; 2. Second testing unit; 3. Electrophoresis capillary; 4. Spectrometer; 5. Laser; 6. First condenser; 7. Cutoff filter; 8. Dichroic mirror; 9. Second condenser; 10. Mirror; 11. Filter. Detailed Implementation

[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] like Figure 1-2As shown, this embodiment provides a dual-channel spectral detection device for capillary electrophoresis, including an electrophoresis capillary 3. A first detection mechanism 1 and a second detection mechanism 2 are arranged on the outside of the electrophoresis capillary 3. The first detection mechanism 1 and the second detection mechanism 2 are arranged perpendicularly to each other on the outside of the electrophoresis capillary 3. A second condenser 9 is connected to one end of the first detection mechanism 1 and the second detection mechanism 2 near the end of the electrophoresis capillary 3. A dichroic mirror 8 is arranged on one side of the second condenser 9 inside the second detection mechanism 2. A reflector 10 is arranged directly below the dichroic mirror 8. The dichroic mirror 8 and the reflector 10 are inclined inside the second detection mechanism 2, and the inclination angles are equal. A cutoff filter 7 is installed on the side of the dichroic mirror 8 away from the second condenser 9. A filter 11 is arranged directly below the cutoff filter 7.

[0019] like Figure 1-2 As shown, a spectrometer 4 is mounted on one side of the cutoff filter 7, and a first condenser 6 is positioned between the cutoff filter 7 and the spectrometer 4. The axis of the spectrometer 4 coincides with the center of the first condenser 6. A laser 5 is mounted on the side of the filter 11 away from the reflector 10, and the axis of the laser 5 coincides with the center of the filter 11. The first detection mechanism 1 and the second detection mechanism 2 have identical structures. At least one set of the first detection mechanism 1 and the second detection mechanism 2 is mounted on the outer side of the electrophoresis capillary 3. Both the spectrometer 4 and the laser 5 are existing products; for example, the spectrometer 4 is model AQ6360, and the laser 5 is model LM12-AOS200.

[0020] The working principle of this invention is as follows: Laser 5 emits a laser beam of a specific wavelength, which is guided by filter 11, reflector 10, dichroic mirror 8, and second condenser 9 to irradiate the electrophoresis capillary 3. Different dyes are labeled on primers at different target sites on the electrophoresis capillary 3. Then, PCR (polymerase chain reaction) primers labeled with multiple fluorescent dyes are mixed and amplified. The amplified products are denatured and electrophoresed into the interior of the electrophoresis capillary 3. Then, under the action of the laser emitted by laser 5, fluorescence in a specific range within the electrophoresis capillary 3 is excited. The fluorescence is reflected to dichroic mirror 8 and transmitted to cutoff filter 7. Under the action of first condenser 6, it is irradiated into the interior of spectrometer 4. The information of the original sample is obtained by collecting the fluorescence signal in a specific range.

[0021] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0022] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A dual-channel spectral detection device for capillary electrophoresis, comprising an electrophoresis capillary (3), characterized in that, The electrophoretic capillary (3) is provided with a first detection mechanism (1) and a second detection mechanism (2) on its outer side. The first detection mechanism (1) and the second detection mechanism (2) are arranged perpendicular to each other on the outer side of the electrophoretic capillary (3). The first detection mechanism (1) and the second detection mechanism (2) are connected to a second focusing plate (9) at the end of the first detection mechanism (1) and the second detection mechanism (2) near the electrophoretic capillary (3). A dichroic mirror (8) is provided on one side of the second condenser (9) inside the second detection mechanism (2). A reflector (10) is provided directly below the dichroic mirror (8). A cutoff filter (7) is installed on the side of the dichroic mirror (8) away from the second condenser (9). A filter (11) is provided directly below the cutoff filter (7). A spectrometer (4) is provided on one side of the cutoff filter (7), a first focusing plate (6) is provided between the cutoff filter (7) and the spectrometer (4), and a laser (5) is provided on the side of the filter (11) away from the reflector (10). The dichroic mirror (8) and the reflector (10) are inclined inside the second detection mechanism (2) and the inclination angles are equal. The axis of the spectrometer (4) coincides with the center of the first focusing plate (6), and the axis of the laser (5) coincides with the center of the filter (11).

2. The dual-channel spectral detection device for capillary electrophoresis according to claim 1, characterized in that, The first detection mechanism (1) and the second detection mechanism (2) have the same structure, and at least one set of the first detection mechanism (1) and the second detection mechanism (2) is provided on the outside of the electrophoresis capillary (3).

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