An integrated polymerase chain reaction detection device

Abstract

The present invention discloses an integrated polymerase chain reaction detection device, which includes a flat plate reactor, a sampling rod and a detection rod. The flat plate reactor includes a flat plate body and a sealing film. A reaction groove is formed on the flat plate body, and a sampling hole and a sample outlet hole are respectively formed on the reaction groove. The sealing film seals the reaction groove to form a reaction cavity. The sampling rod includes a sampling rod body, and a sample chamber is formed at one end of the sampling rod body. The detection rod includes a detection rod body, and a test strip groove is formed on the detection rod body. The sampling rod body and the detection rod body are respectively rotatably connected to the flat plate body. When the sampling rod body rotates to the communication position, the sample chamber is communicated with the sampling hole. When the sampling rod body rotates to the isolation position, the sample chamber is not communicated with the sampling hole. When the detection rod body rotates to the communication position, the test strip groove is communicated with the sample outlet hole. When the detection rod body rotates to the isolation position, the test strip groove is not communicated with the sample outlet hole. The device has a simple structure and high integration degree, integrating the polymerase chain reaction and detection together.

Description

Technical Field

[0001] The present invention relates to the technical field of polymerase chain reaction devices, and particularly relates to an integrated polymerase chain reaction detection device. Background Art

[0002] The full name of the polymerase chain reaction detection technology is fluorescence quantitative polymerase chain reaction. As the most common method for detecting virus-specific sequences, it was first proposed in the 1970s. Finally, an American company invented the polymerase chain reaction, which simulated the in vivo DNA replication process with a heat-resistant DNA polymerase. Nowadays, the development has enabled the amplification of a specific DNA fragment in vitro. The specific process of the polymerase chain reaction is as follows: First, high-temperature denaturation is used to heat the template DNA double-strand hydrogen bonds to break and obtain two single strands. Low-temperature annealing allows the suddenly cooled template DNA to complementarily bind to the primer according to the base pairing principle. Extension at an appropriate temperature allows the DNA polymerase to re-bind at a suitable temperature to form a new DNA strand complementary to the template. Such a cycle is repeated to copy the DNA repeatedly, and finally, the DNA band of the specific region is amplified.

[0003] Under the background of the polymerase chain reaction detection technology, various types of automated polymerase chain reaction thermal cyclers have been gradually invented, and the corresponding consumables used have also become one of the inspection standards for determining reaction efficiency, the number of detections, the stability of the detection process, and the independence of the detection object. In today's era of highly integrated technology and mechanical automation, fully automatic and highly concentrated practical instruments have gradually become the core direction of technology optimization.

[0004] Currently, in China, most inventions and designs are focused on the polymerase chain reaction instrument itself, and the consumables for polymerase chain reaction detection are mostly centrifuge tubes, which require manual operation. Each centrifuge tube is independent, and the operation is cumbersome. Currently, the optimizations for the fully automatic and integrated directions are mostly designed for sample liquid transfer and mixing, and nested on the basis of centrifuge tube shapes. However, such methods have the following problems: 1. Lack of structural flexibility, making it inconvenient to add new substances or new processes; 2. Some steps such as detection sampling still have human intervention; 3. There is a possibility of contamination due to non-full-process closure; 4. The independence deviation of each reaction area may cause cross-influence. The polymerase chain reaction detection has extremely high temperature control requirements, high biological purity requirements, needs to be sensitive enough to withstand changing temperatures, and introducing impurities is not allowed in most experiments. Therefore, the independence and integrity requirements of the entire reaction device are very high. Summary of the Invention

[0005] Based on the above-mentioned prior art, the present invention provides an integrated polymerase chain reaction detection device. The device has a simple structure, reasonable design, and high integration degree. It integrates polymerase chain reaction and detection together, and with the cooperation of an external power machine, it can achieve full-process full automation without manual intervention.

[0006] The technical solution adopted to achieve the above object of the present invention is as follows:

[0007] An integrated polymerase chain reaction detection device includes a flat reactor, a sampling rod, and a detection rod. The flat reactor includes a flat body and a sealing film. A reaction groove is formed on one surface of the flat body. An inlet hole and an outlet hole are respectively formed at the bottom of the reaction groove. The sealing film seals the surface of the flat body where the reaction groove is formed. The enclosed space formed by the sealing film and the reaction groove is a reaction chamber. The sampling rod includes a sampling rod body, and a sample chamber is formed at one end of the sampling rod body. The detection rod includes a detection rod body, and a test strip groove is formed along the length direction of the detection rod body. The sampling rod body and the detection rod body are respectively rotatably connected to the other surface of the flat body. When the sampling rod body rotates to the connection position, the bottom of the sample chamber communicates with the inlet hole. When the sampling rod body rotates to the isolation position, the bottom of the sample chamber does not communicate with the inlet hole. When the detection rod body rotates to the connection position, the test strip groove communicates with the outlet hole. When the detection rod body rotates to the isolation position, the test strip groove does not communicate with the outlet hole.

[0008] The bottom of the sample chamber is conical, and the bottom of the sample chamber is open. A first clamping groove is provided at a position on the side wall of the sampling rod body corresponding to the bottom of the sample chamber. A first silica gel sealing block is clamped in the first clamping groove. A first communication hole is provided on one side of the first silica gel sealing block. One end of the first communication hole communicates with the bottom of the sample chamber. When the sampling rod body rotates to the connection position, the bottom of the sample chamber communicates with the inlet hole through the first communication hole. When the sampling rod body rotates to the isolation position, the other side of the first silica gel sealing block seals the inlet hole;

[0009] A second clamping groove is formed on the side wall of the detection rod body. A second silica gel sealing block is clamped in the second clamping groove. A second communication hole is provided on one side of the second silica gel sealing block. One end of the second communication hole communicates with the sample contact end at the bottom of the test strip groove. When the detection rod body rotates to the connection position, the test strip groove communicates with the outlet hole. When the detection rod body rotates to the connection position, the sample contact end of the test strip groove communicates with the outlet hole through the second communication hole. When the detection rod body rotates to the isolation position, the other side of the second silica gel sealing block seals the outlet hole.

[0010] Both the first silica gel sealing block and the second silica gel sealing block are wedge-shaped. The first communication hole is provided on the thicker side of the first silica gel sealing block, and the axis of the first communication hole is perpendicular to the length direction of the sampling rod body. The second communication hole is provided on the thicker side of the second silica gel sealing block, and the axis of the second communication hole is perpendicular to the length direction of the detection rod.

[0011] The reaction groove is in a quasi-elliptical shape with pointed ends at both ends. The inlet hole and the outlet hole are respectively formed at the two tips at the bottom of the reaction groove. The flat body is square, and the two tips of the reaction groove are respectively close to a pair of diagonals of the flat body.

[0012] On two edges of the other surface of the flat plate body in the length direction, a first enclosing plate and a second enclosing plate are respectively provided. The first enclosing plate and the second enclosing plate are symmetrically distributed. Both the first enclosing plate and the second enclosing plate are in the shape of a superior arc bow. On two opposite edges of the other surface of the flat plate body, a first placement groove and a second placement groove are respectively provided. Both the first placement groove and the second placement groove are in the shape of an inferior arc bow. The sample injection hole is communicated with the first placement groove, and the sample output hole is communicated with the second placement groove. The space formed by the first enclosing plate and the first placement groove constitutes a first sleeve, and the space formed by the second enclosing plate and the second placement groove constitutes a second sleeve. The sample injection rod body movably penetrates through the first sleeve, and the detection rod body movably penetrates through the second sleeve.

[0013] A first protrusion is provided at the bottom of the first placement groove. A first limiting groove is provided on the side wall of the sample injection rod body. A first sliding groove is provided at the bottom of the first limiting groove. The first protrusion can slide along the first sliding groove. When the sample injection rod body rotates to the communication position, the first protrusion slides to a position where it contacts one inner side wall of the first limiting groove. When the sample injection rod body rotates to the isolation position, the first protrusion slides to a position where it contacts the other inner side wall of the first limiting groove.

[0014] A second protrusion is provided in the second placement groove. A second limiting groove is provided on the side wall of the detection rod body. A second sliding groove is provided at the bottom of the second limiting groove. The second protrusion can slide along the second sliding groove. When the detection rod body rotates to the communication position, the second protrusion slides to a position where it contacts one inner side wall of the second limiting groove. When the detection rod body rotates to the isolation position, the second protrusion slides to a position where it contacts the other inner side wall of the second limiting groove.

[0015] A first connecting male head is provided at the other end of the sample injection rod body. A second connecting male head is provided at one end of the detection rod body close to the sample contact end of the test strip groove. Both the first connecting male head and the second connecting male head are in the shape of a straight line.

[0016] A sealing cover is provided at the sample inlet of the sample chamber.

[0017] The sealing film is a heat-sealing film, and the reaction tank is sealed by hot pressing with the sealing film.

[0018] Compared with the prior art, the beneficial effects and advantages of the present invention are as follows:

[0019] 1. The device has a simple structure, low manufacturing cost, and ingenious design. It only needs three parts to complete the polymerase chain reaction and detection, greatly simplifying the operation process and being convenient to operate.

[0020] 2. The reaction tank of the device is sealed with a heat-sealing film to form a reaction chamber. Due to the presence of the heat-sealing film, the sample injection power of the device is rather special. When the reaction chamber is connected to the sample chamber and both the reaction chamber and the sample injection tube are isolated from the outside, vibrating the heat-sealing film with external machinery or directly pressing the heat-sealing film by hand can achieve gas replacement between the upper-layer air of the sample and the air inside the reaction chamber, allowing the sample in the sample chamber to enter the reaction chamber and the air in the reaction chamber to enter the sample chamber. This operation can ensure the independence and purity of the reaction.

[0021] 3. When the device realizes the detection function, rotate the detection rod to connect the test strip slot with the sample outlet hole. Under the action of gravity and the attraction of the test strip, smooth liquid discharge can be ensured, enabling the reacted liquid to enter the test strip slot to react with the test strip for detection.

[0022] 4. Due to the presence of the heat-sealing film, the device can quickly heat up and cool down, thereby improving the reaction efficiency.

[0023] 5. Under the action of an external power machine, the device can realize the full automation of the entire process of polymerase chain reaction and detection without manual intervention, thus eliminating the possibility of contamination.

[0024] 6. When the device is in use, place the plate reactor vertically with the liquid inlet hole at the uppermost part of the reaction chamber and the liquid outlet hole at the lowermost part of the reaction chamber. Since the bottom of the sample injection chamber is connected to the liquid inlet hole and the liquid outlet hole is connected to the test strip slot, during sample injection, continuously press or vibrate the sealing film, and under the action of gravity and gas exchange, the liquid can quickly enter the reaction chamber without reservation. During detection, smooth liquid discharge can be ensured under the action of gravity and the attraction of the test strip.

[0025] 7. When the sample and the reaction reagent are compatible, the device can complete the entire process of sample injection, reaction, and detection in one integrated unit and can be widely applied to the polymerase chain reaction detection of various samples.

[0026] 8. The device is made by 3D printing with food-grade polypropylene plastic, with a low cost. At the same time, it is a disposable product, which can effectively avoid cross-infection, eliminate the cleaning step, and greatly save the measurement cost. Description of the Drawings

[0027] Figure 1 It is a schematic structural diagram of an integrated polymerase chain reaction detection device.

[0028] Figure 2 It is a schematic structural diagram of an integrated polymerase chain reaction detection device (at a different Figure 1 angle).

[0029] Figure 3 It is a schematic structural diagram of the sample injection rod.

[0030] Figure 4 is the front view of Figure 3 .

[0031] Figure 5 is the structural schematic diagram of the flat plate reactor.

[0032] Figure 6 Figure 5 is the rear view of

[0033] Figure 7 is the structural schematic diagram of the detection rod.

[0034] Figure 8 is Figure 7 's front view.

[0035] Figure 9 is Figure 7 's rear view.

[0036] Among them, 1 - flat plate body, 2 - heat-sealing film, 3 - reaction tank, 4 - sample injection hole, 5 - sample outlet hole, 6 - sample injection rod body, 7 - sample bin, 8 - sealing cover, 9 - first card slot, 10 - first silica gel sealing block, 11 - first communication hole, 12 - detection rod body, 13 - test paper slot, 14 - second card slot, 15 - second silica gel sealing block, 16 - second communication hole, 17 - first enclosing plate, 18 - second enclosing plate, 19 - first protrusion, 20 - first limiting groove, 21 - first sliding groove, 22 - second protrusion, 23 - second limiting groove, 24 - second sliding groove, 25 - first connecting male head, 26 - second connecting male head. Specific Embodiments

[0037] The present invention will be described in detail below with reference to the accompanying drawings.

[0038] The structure of the integrated polymerase chain reaction detection device provided in this embodiment is as shown in Figure 1 and Figure 2 , and it includes a flat plate reactor, a sample injection rod and a detection rod. This device is made by 3D printing, and the production is simple.

[0039] As shown in Figures 5 - 6 , the flat plate reactor includes a flat plate body 1 and a heat-sealing film 2. The flat plate body 1 is square. A reaction tank 3 is provided on one surface of the flat plate body 1. The reaction tank 3 is in an approximately elliptical shape with pointed ends at both ends. The two pointed ends of the bottom of the reaction tank 3 are respectively provided with a sample injection hole 4 and a sample outlet hole 5. The heat-sealing film 2 seals the reaction tank 3 by hot pressing. The closed space surrounded by the heat-sealing film 2 and the reaction tank 3 is the reaction chamber.

[0040] As shown in Figures 3 - 4As shown in the figure, the sampling rod includes a sampling rod body 6. One end of the sampling rod body 6 is provided with a sample chamber 7. A sealing cover 8 is provided at the inlet of the sample chamber 7. The bottom of the sample chamber 7 is conical and has an opening at the bottom. At the other end of the sampling rod body 6, there is a first connecting male head 25. The first connecting male head 25 is in a shape of a straight bar. The purpose of setting the first connecting male head 25 is to facilitate connection with an external power machine to drive the sampling rod body 6 to rotate.

[0041] At a position on the side wall of the sampling rod body 6 corresponding to the bottom of the sample chamber 7, there is a first clamping groove 9. A wedge-shaped first silica gel sealing block 10 is clamped in the first clamping groove 9. A first communication hole 11 is provided on the thicker side of the first silica gel sealing block 10. The axis of the first communication hole 11 is perpendicular to the length direction of the sampling rod body 6, and one end of the first communication hole 11 communicates with the bottom of the sample chamber 7.

[0042] As Figures 7 - 9 shown in the figure, the detection rod includes a detection rod body 12. Along its length direction, a test strip groove 13 is provided on the detection rod body 12. The test strip groove 13 is in a long strip shape and is used for placing test strips. At one end of the detection rod body 12 close to the sample contact end of the test strip groove 13, there is a second connecting male head 26. The second connecting male head 26 is in a shape of a straight bar. The purpose of setting the second connecting male head 26 is to facilitate connection with an external power machine to drive the detection rod body 12 to rotate.

[0043] At a position on the side wall of the detection rod body 12 corresponding to the sample contact end of the test strip groove, there is a second clamping groove 14. A wedge-shaped second silica gel sealing block 15 is clamped in the second clamping groove 14. A second communication hole 16 is provided on the thicker side of the second silica gel sealing block 15. The axis of the second communication hole 16 is perpendicular to the length direction of the detection rod body 12, and one end of the second communication hole 16 communicates with the sample contact end at the bottom of the test strip groove 13.

[0044] On the two edges of the other surface of the flat plate body 1 in the length direction, there are respectively a first enclosing plate 17 and a second enclosing plate 18. The first enclosing plate 17 and the second enclosing plate 18 are symmetrically distributed and are both in the shape of a superior arc. On the two opposite edges of the other surface of the flat plate body 1, there are respectively a first placement groove and a second placement groove. The first placement groove and the second placement groove are both in the shape of an inferior arc. The sampling hole 4 communicates with the first placement groove, and the sample outlet hole 5 communicates with the second placement groove. The space formed by the first enclosing plate 17 and the first placement groove constitutes a first sleeve, and the space formed by the second enclosing plate 18 and the second placement groove constitutes a second sleeve. In this embodiment, in order to make the device more beautiful and convenient for observation, through grooves are opened on the first enclosing plate 17 and the second enclosing plate 18 to form an observation window.

[0045] The bottom of the first placement groove is provided with a first protrusion 19, and a first limiting groove 20 is provided on the side wall of the sample injection rod body. The bottom of the first limiting groove 20 is provided with a first sliding groove 21. The first protrusion 19 can slide along the first sliding groove 21, and the first protrusion 19 is limited by the two opposite inner side walls of the first limiting groove 20. The sample injection rod body 6 movably penetrates through the first sleeve. When the sample injection rod body 6 rotates to the communication position, the bottom of the sample chamber 7 is communicated with the sample injection hole 4 through the first communication hole 11. At this time, the first protrusion 19 slides to a position where it contacts one inner side wall of the first limiting groove 20. When the sample injection rod body 6 rotates to the isolation position, the other side of the first silicone sealing block 10 seals the sample injection hole 4, and the sealing performance is ensured by the silicone sealing block. At this time, the first protrusion 19 slides to a position where it contacts the other inner side wall of the first limiting groove 20.

[0046] The bottom of the second placement groove is provided with a second protrusion 22, and a second limiting groove 23 is provided on the side wall of the detection rod body. The bottom of the second limiting groove 23 is provided with a second sliding groove 24. The second protrusion 22 can slide along the second sliding groove 24, and the second protrusion 22 is limited by the two opposite inner side walls of the second limiting groove 23. The detection rod body 12 movably penetrates through the second sleeve. When the detection rod body 12 rotates to the communication position, the sample contact end of the test paper slot 13 is communicated with the sample outlet hole 5 through the second communication hole 16. At this time, the second protrusion 22 slides to a position where it contacts one inner side wall of the second limiting groove 23. When the detection rod body 12 rotates to the isolation position, the other side of the second silicone sealing block 15 seals the sample outlet hole 5, and the sealing performance is ensured by the silicone sealing block. At this time, the second protrusion 22 slides to a position where it contacts the other inner side wall of the second limiting groove 23.

[0047] The usage method of the above-mentioned integrated polymerase chain reaction detection device is as follows:

[0048] 1. Vertically place the flat reactor, and manually or through an external power machine rotate the sample injection rod body 6 and the detection rod body 12 so that both the sample injection rod body 6 and the detection rod body 12 are in the isolation position. At this time, the first silicone sealing block 10 seals the sample injection hole, and the second silicone sealing block 15 seals the sample outlet hole;

[0049] 2. Manually or through an external power machine rotate the sample injection rod body 6 so that the sample injection rod body 6 rotates to the communication position. The bottom of the sample chamber 7 is communicated with the sample injection hole 4 through the first communication hole 11, that is, the sample chamber 7 is communicated with the reaction chamber. By manually continuously pressing the heat-sealing film 2 or through external mechanical vibration of the heat-sealing film 2, the air in the reaction chamber is replaced with the sample in the sample chamber 7, so that the sample in the sample chamber 7 enters the reaction chamber;

[0050] 3. After the sample enters the reaction chamber, a reaction process of high-temperature denaturation and low-temperature annealing is carried out. If additional reaction enzymes are needed, manually or through an external power mechanical rotation of the sampling rod body 6, rotate the sampling rod body 6 to the communication position. The bottom of the sample chamber 7 is communicated with the sampling hole 4 through the first communication hole 11, that is, the sample chamber 7 is communicated with the reaction chamber. By continuously pressing the heat-sealing film 2 manually or through external mechanical vibration of the heat-sealing film 2, the gas in the reaction chamber is reduced and the pressure becomes smaller. The reaction enzyme is sucked into the reaction chamber through the external pressure. After the reaction enzyme is completely sucked in, manually or through an external power mechanical rotation of the sampling rod body 6, make the sampling rod body 6 in the isolation position. At this time, the first silicone seal block 10 seals the sampling hole 4, and the sample chamber 7 is isolated from the reaction chamber. By operating like this, additional reactants can be added in the middle;

[0051] 4. After all the reactions are completed, manually or through an external power mechanical rotation of the detection rod body 12, rotate the detection rod body 12 to the communication position. The sample contact end of the test strip slot 13 is communicated with the sample outlet hole 5 through the second communication hole 16, that is, the reaction chamber is communicated with the test strip slot 13. Through the liquid-absorbing ability of the test strip, by continuously pressing the heat-sealing film 2 manually or through external mechanical vibration of the heat-sealing film 2, the reaction chamber is pressed. Coupled with the liquid-absorbing ability of the test strip, the liquid in the reaction chamber is transferred into the test strip. The reaction condition of the corresponding test strip can be detected by machine vision, and the result is then fed back to the computer.

Claims

1. An integrated polymerase chain reaction detection device, characterized in that: it includes a flat reactor, a sampling rod and a detection rod. The flat reactor includes a flat body and a sealing film. A reaction groove is formed on one surface of the flat body. An injection hole and a sampling hole are respectively formed at the bottom of the reaction groove. The sealing film seals the surface of the flat body where the reaction groove is formed. The closed space surrounded by the sealing film and the reaction groove is the reaction chamber. The sampling rod includes a sampling rod body, and a sample chamber is formed at one end of the sampling rod body. The detection rod includes a detection rod body, and a test strip groove is formed along the length direction of the detection rod body. The sampling rod body and the detection rod body are respectively rotatably connected to the other surface of the flat body; When the sampling rod body rotates to the connection position, the bottom of the sample chamber is communicated with the injection hole. By continuously pressing the sealing film manually or by external mechanical vibration of the sealing film, the air in the reaction chamber is replaced with the sample in the sample chamber, so that the sample in the sample chamber enters the reaction chamber. When the sampling rod body rotates to the isolation position, the bottom of the sample chamber is not communicated with the injection hole. When the detection rod body rotates to the connection position, the test strip groove is communicated with the sampling hole. By continuously pressing the sealing film manually or by external mechanical vibration of the sealing film, the reaction chamber is pressed, and the liquid in the reaction chamber is transferred onto the test strip. When the detection rod body rotates to the isolation position, the test strip groove is not communicated with the sampling hole. The reaction groove is in a quasi-elliptical shape with pointed ends at both ends. The injection hole and the sampling hole are respectively formed at the two tips of the bottom of the reaction groove. When the flat reactor is placed vertically, the injection hole is located at the highest position of the reaction chamber, and the sampling hole is located at the lowest position of the reaction chamber.

2. The integrated polymerase chain reaction detection device according to claim 1, characterized in that: the bottom of the sample chamber is conical, the bottom of the sample chamber is open, and a first clamping groove is provided at a position on the side wall of the sampling rod body corresponding to the bottom of the sample chamber. A first silica gel sealing block is clamped in the first clamping groove. A first communication hole is provided on one side of the first silica gel sealing block. One end of the first communication hole is communicated with the bottom of the sample chamber. When the sampling rod body rotates to the connection position, the bottom of the sample chamber is communicated with the injection hole through the first communication hole. When the sampling rod body rotates to the isolation position, the other side of the first silica gel sealing block seals the injection hole; a second clamping groove is formed on the side wall of the detection rod body. A second silica gel sealing block is clamped in the second clamping groove. A second communication hole is provided on one side of the second silica gel sealing block. One end of the second communication hole is communicated with the sample contact end at the bottom of the test strip groove. When the detection rod body rotates to the connection position, the test strip groove is communicated with the sampling hole. When the detection rod body rotates to the connection position, the sample contact end of the test strip groove is communicated with the sampling hole through the second communication hole. When the detection rod body rotates to the isolation position, the other side of the second silica gel sealing block seals the sampling hole.

3. The integrated polymerase chain reaction detection device according to claim 2, characterized in that: The first silicone sealing block and the second silicone sealing block are both wedge-shaped. The first communication hole is arranged on the thicker side of the first silicone sealing block, and the axis of the first communication hole is perpendicular to the length direction of the sampling rod body. The second communication hole is arranged on the thicker side of the second silicone sealing block, and the axis of the second communication hole is perpendicular to the length direction of the detection rod.

4. The integrated polymerase chain reaction detection device according to claim 1, characterized in that: The flat plate body is square, and the two tips of the reaction tank are respectively close to a pair of diagonals of the flat plate body.

5. The integrated polymerase chain reaction detection device according to claim 1, characterized in that: On the two edges in the length direction of the other surface of the flat plate body, a first enclosing plate and a second enclosing plate are respectively provided. The first enclosing plate and the second enclosing plate are symmetrically distributed. Both the first enclosing plate and the second enclosing plate are in the shape of a superior arc. On the two opposite edges of the other surface of the flat plate body, a first placement groove and a second placement groove are respectively provided. Both the first placement groove and the second placement groove are in the shape of an inferior arc. The sampling hole communicates with the first placement groove, and the sample outlet hole communicates with the second placement groove. The space formed by the first enclosing plate and the first placement groove constitutes a first sleeve, and the space formed by the second enclosing plate and the second placement groove constitutes a second sleeve. The sampling rod body movably penetrates through the first sleeve, and the detection rod body movably penetrates through the second sleeve.

6. The integrated polymerase chain reaction detection device according to claim 5, characterized in that: A first protrusion is provided at the bottom of the first placement groove. A first limiting groove is provided on the side wall of the sampling rod body. A first sliding groove is provided at the bottom of the first limiting groove. The first protrusion can slide along the first sliding groove. When the sampling rod body rotates to the communication position, the first protrusion slides to a position where it contacts one inner side wall of the first limiting groove. When the sampling rod body rotates to the isolation position, the first protrusion slides to a position where it contacts the other inner side wall of the first limiting groove. A second protrusion is provided in the second placement groove. A second limiting groove is provided on the side wall of the detection rod body. A second sliding groove is provided at the bottom of the second limiting groove. The second protrusion can slide along the second sliding groove. When the detection rod body rotates to the communication position, the second protrusion slides to a position where it contacts one inner side wall of the second limiting groove. When the detection rod body rotates to the isolation position, the second protrusion slides to a position where it contacts the other inner side wall of the second limiting groove.

7. The integrated polymerase chain reaction detection device according to claim 6, characterized in that: A first connecting male head is provided at the other end of the sampling rod body. A second connecting male head is provided at one end of the detection rod body close to the sample contact end of the test strip slot. Both the first connecting male head and the second connecting male head are in the shape of a straight line.

8. The integrated polymerase chain reaction detection device according to claim 1, characterized in that: A sealing cover is provided on the sample chamber sampling port.

9. The integrated polymerase chain reaction detection device according to claim 1, characterized in that: The sealing film is a heat-sealing film, and the reaction tank is sealed by hot pressing the sealing film.

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