A kit for in vitro expansion of nk cells

Abstract

The present application relates to the technical field of kits, in particular to a kind of NK cell in vitro expansion kit, including NK cell kit body, NK cell kit body is equipped with separating plate in the middle, the two sides of separating plate are reagent bottle storage cavity and cell culture cavity respectively, the top of reagent bottle storage cavity is equipped with first isolation plate, and the top of cell culture cavity is equipped with second isolation plate.The present application is equipped with reagent bottle storage cavity and cell culture cavity in NK cell kit body, which can facilitate the culture of cells, and facilitate the storage of reagent bottles;By being equipped with jacking block in reagent bottle storage cavity, the reagent bottle in storage groove can be jacked up and ejected from the storage groove by the upward movement of jacking block driven by the downward pressure of pressure rod, so that the reagent bottle can be easily taken, and the operability is strong;By being equipped with magnetic baffle in the symmetry in universal ball, after the dropper is pulled out of the through hole, the magnetic baffle can be automatically closed under the action of magnetic force.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of kits, in particular to an NK cell in-vitro expansion kit. BACKGROUND

[0002] NK cells are important immune cells in the body, not only related to anti-tumor, anti-viral infection and immune regulation, but also involved in the occurrence of hypersensitivity and autoimmune diseases in some cases, capable of recognizing target cells, killing mediators, derived from bone marrow lymphoid stem cells, differentiation and development dependent on bone marrow and thymus microenvironment, mainly distributed in bone marrow, peripheral blood, liver, spleen, lung and lymph nodes, NK cells are different from T and B cells, a type of lymphocyte that can non-specifically kill tumor cells and virus-infected cells without prior sensitization.

[0003] Currently, NK cells are used for immunotherapy in medicine, which requires first expanding a large number of NK cells with high killing activity in vitro. Therefore, NK cell kits are widely used, but the traditional kits have poor sealing, and are easily affected by the external environment during the expansion of NK cells. At the same time, the most suitable temperature for NK cell culture is 37 degrees Celsius, and the traditional kits cannot constantly maintain the temperature in the box at 37 degrees Celsius, making it difficult to ensure the expansion of NK cells.

[0004] CN202110636144.6, an NK cell in-vitro expansion kit, includes a box body, a box cover, a culture dish, a sealing plate, a universal ball and a dropper, the box body is hinged at the top with the box cover, the inner bottom of the box body is provided with a plurality of placing grooves, each placing groove is provided with a culture dish; the inner upper end of the box body is installed with a sealing plate, the center of the sealing plate is provided with a universal ball mounting hole, the universal ball mounting hole is provided with a universal ball, the universal ball is provided with a through hole, and the through hole is provided with a dropper. The beneficial effects of the present application are: when it is necessary to add cells to a certain culture dish, the dropper can be rotated in any direction under the action of the universal ball, the lower end of the dropper is directed to the corresponding culture dish to add culture medium, the operation is convenient, the sealing plate does not need to be opened, and the NK cells in the culture dish can be prevented from being contaminated. The application stores and takes cells by rotating the dropper in the universal ball; when the dropper is pulled out of the universal ball, the through hole in the universal ball will make the air in the box body contact with the air outside, so that the cells are easily contaminated, which has certain limitations.

[0005] However, the existing NK cell kit can only culture cells or store reagent bottles, and cannot have both functions. After storing the reagent bottle, the existing NK cell kit needs to be taken out from the storage groove by using a clamp tool such as a tweezers. When the tweezers cannot be found, the reagent bottle is not convenient to take out from the storage groove, which has certain limitations. SUMMARY

[0006] The main purpose of the present application is to provide an NK cell in vitro expansion kit to solve the problem that the existing NK cell kit does not have the function of culturing cells and storing reagent bottles, and to solve the problem that the existing NK cell kit is easy to be contaminated by air contact during cell culture, and the problem that it is not convenient to take out the reagent bottle from the storage slot.

[0007] In order to achieve the above purpose, the present application provides the following technology:

[0008] An NK cell in vitro expansion kit, comprising an NK cell kit body, wherein a separation plate is arranged in the middle of the NK cell kit body, and the two sides of the separation plate are respectively a reagent bottle storage cavity and a cell culture cavity:

[0009] The top end of the reagent bottle storage cavity is provided with a first isolation plate, at least two storage slots are installed on the first isolation plate, a limiting cavity is opened on the first isolation plate, a pressure rod is movably arranged in the limiting cavity, a flap is hinged to the bottom end of the pressure rod, a horizontal plate is hinged to the end of the flap away from the pressure rod, and top lifting blocks are hinged to the two ends of the horizontal plate, and the two ends of the top lifting blocks penetrate into the two storage slots arranged longitudally;

[0010] A second isolation plate is installed at the top end of the cell culture cavity, a universal ball is movably installed in the second isolation plate, a dropper is movably installed on the universal ball, a through hole is opened in the universal ball, the dropper is movably installed in the through hole, a plurality of magnetic baffles are symmetrically hinged in the through hole, a plurality of partition plates are arranged in the cell culture cavity, and a plurality of temperature controllers are uniformly arranged in the cell culture cavity.

[0011] Further, a cover is hinged to the end of the NK cell kit body, a sealing film is installed on the inner side of the cover, and the sealing film is arranged in close contact with the top end of the NK cell kit body.

[0012] Further, a placing slot is opened on the first isolation plate, a spare pressure rod is movably placed in the placing slot, an installation hole is arranged at the top end of the pressure rod, and the spare pressure rod can be inserted into the installation hole.

[0013] Further, a third hinge is installed in the middle of the bottom surface of the flap, a support column is installed at the bottom end of the inside of the reagent bottle storage cavity, and the bottom end of the third hinge is hinged to the support column.

[0014] Further, a second hinge is installed at the end of the flap, the flap and the horizontal plate are hinged through the second hinge, first hinges are installed at the two ends of the horizontal plate, and the bottom end of the top lifting block is hinged to the horizontal plate through the first hinge.

[0015] Further, a hole is opened at the bottom end of the storage slot, and the top lifting block penetrates through the hole.

[0016] Further, the inner wall of the storage groove is abutted by a plurality of reset springs, one side of the reset spring away from the inner wall of the storage groove is abutted by a clip, the clips are arranged in groups, two clips form a group, and each group of clips is arranged symmetrically.

[0017] Further, the clip is arranged in an arc shape, and a flexible sponge is embedded in the inner side of the clip.

[0018] Further, the side of the NK cell kit body is provided with an observation window opening into the cell culture cavity.

[0019] Compared with the prior art, the present application can bring the following technical effects:

[0020] 1. By arranging the reagent bottle storage cavity and the cell culture cavity in the NK cell kit body, the cell can be cultured, and the reagent bottle can be stored conveniently.

[0021] 2. By arranging the jacking block in the reagent bottle storage cavity, the reagent bottle in the storage groove can be jacked up and ejected from the storage groove by pressing the pressing rod and driving the jacking block to move upward, so that the reagent bottle can be taken conveniently, and the operability is strong.

[0022] 3. By arranging the temperature controller in the cell culture cavity, the cell culture cavity can be controlled in temperature, so that the culture of the cells in the cell culture cavity is facilitated; by arranging the universal ball and the dropper, the cells or the culture solution cultured in the cell culture cavity can be taken out or put in conveniently.

[0023] 4. By arranging the magnetic baffle symmetrically in the universal ball, after the dropper is pulled out of the through hole, the magnetic baffle is automatically closed under the action of the magnetic force, the contact between the cell culture cavity and the external air is reduced, and the internal part of the cell culture cavity is prevented from being polluted. BRIEF DESCRIPTION OF DRAWINGS

[0024] The accompanying drawings, which form a part of this application, are intended to provide further understanding of the application and are incorporated herein for a purpose of explanations and are not intended as an undue limitation of the application. The above-mentioned and other features and advantages of the application will become more apparent as the application becomes better understood with reference to the accompanying drawings, wherein:

[0025] Figure 1 is a schematic diagram of the overall structure of the present application;

[0026] Figure 2 is a partial sectional view of the reagent bottle storage cavity in the present application;

[0027] Figure 3 is a partial sectional view of the cell culture cavity in the present application;

[0028] Figure 4is a cross-sectional view of the universal ball in the present application.

[0029] In the figure: 1-NK cell kit body, 2-reagent bottle storage cavity, 3-cell culture cavity, 4-lid, 5-sealing film, 6-universal ball, 601-magnetic baffle, 602-through hole, 7-dropper, 8-observation window, 9-putting groove, 10-storage groove, 11-reset spring, 12-clip, 13-lifting block, 14-first hinge, 15-cross plate, 16-second hinge, 17-flap, 18-strut, 19-third hinge, 20-limiting cavity, 21-pressing rod, 22-first isolation plate, 23-temperature controller, 24-partition, 25-second isolation plate, 26-backup pressing rod. DETAILED DESCRIPTION

[0030] In order to make the personnel in the technical field better understand the present application scheme, the technical scheme in the embodiment of the present application will be described clearly and completely in the following with the aid of the drawings in the embodiment of the present application. Obviously, the described embodiment is only a part of the embodiment of the present application, not all. Based on the embodiment in the present application, all other embodiments obtained by the person skilled in the art without creative labor should belong to the scope of protection of the present application.

[0031] It should be noted that the terms "first", "second" and the like in the specification and claims of the present application and the above-described drawings are used to distinguish similar objects, and do not necessarily have to describe a specific order or sequence. It should be understood that the data thus used can be interchanged under appropriate circumstances, so that the embodiments of the present application described herein can be implemented. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion, for example, a process, method, system, product or device including a series of steps or units does not have to be limited to those steps or units clearly listed, but can include other steps or units not clearly listed or inherent to these processes, methods, products or devices.

[0032] In the present application, the terms "up", "down", "left", "right", "front", "back", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. These terms are mainly used to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to have a specific orientation, or to be constructed and operated in a specific orientation.

[0033] And, the above-mentioned partial terms can be used to represent other meanings in addition to the orientation or positional relationship, for example, the term "upper" can also be used to represent a certain dependent relationship or connection relationship in some cases. For those skilled in the art, the specific meaning of these terms in the present application can be understood according to the specific circumstances.

[0034] In addition, the meaning of the term "a plurality of" should be two and more than two.

[0035] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict. The present application will be described in detail below with reference to the drawings and in combination with the embodiments.

[0036] Embodiment 1, a NK cell in vitro expansion kit, comprising an NK cell kit body 1, the middle of the NK cell kit body 1 is provided with a separation plate, and the two sides of the separation plate are respectively a reagent bottle storage cavity 2 and a cell culture cavity 3: the top of the reagent bottle storage cavity 2 is provided with a first isolation plate 22, at least two storage grooves 10 are installed on the first isolation plate 22, a limiting cavity 20 is opened on the first isolation plate 22, a pressure rod 21 is movably arranged in the limiting cavity 20, the bottom end of the pressure rod 21 is hinged with a flap 17, the end away from the pressure rod 21 of the flap 17 is hinged with a horizontal plate 15, the two ends of the horizontal plate 15 are hinged with jacking blocks 13, and the two ends of the jacking blocks 13 penetrate into the two storage grooves 10 arranged longitudinally; by arranging the storage groove 10, the reagent bottle can be stored in the storage groove 10, and by pressing the pressure rod 21, the end away from the pressure rod 21 of the flap 17 is raised, driving the jacking blocks 13 to be lifted upwards, the reagent bottle placed in the storage groove 10 can be lifted upwards by the lifting of the jacking blocks 13, and then the reagent bottle is convenient to take out without the aid of other tools, which is convenient to operate.

[0037] The top of the cell culture cavity 3 is installed with a second isolation plate 25, a universal ball 6 is movably installed in the second isolation plate 25, a dropper 7 is movably installed on the universal ball 6, a through hole 602 is opened in the universal ball 6, the dropper 7 is movably installed in the through hole 602, a plurality of magnetic baffles 601 are symmetrically hinged in the through hole 602, a plurality of partition plates 24 are arranged in the inside of the cell culture cavity 3, and a plurality of temperature controllers 23 are uniformly arranged in the inside of the cell culture cavity 3; by arranging the universal ball 6, the dropper 7 can be conveniently inserted into the universal ball 6, and then the cells in the cell culture cavity 3 can be accessed and cultured; by arranging the temperature controller 23, the temperature inside the cell culture cavity 3 can be controlled, and the culture of the cells is facilitated; and by arranging the magnetic baffles 601 in the through hole in the universal ball 6, after the dropper 7 is pulled out of the universal ball 6, the magnetic baffles 601 are automatically closed under the action of the magnetic force, thereby ensuring the airtightness of the through hole in the universal ball 6, avoiding the contact between the inside and the outside of the cell culture cavity 3, and affecting the normal culture of the cells.

[0038] In the embodiment 2, the bottom end of the storage groove 10 is provided with a hole, and the jacking block 13 passes through the hole; the inner wall of the storage groove 10 is abutted by a plurality of reset springs 11, the side of the reset spring 11 away from the inner wall of the storage groove 10 is abutted by a clip 12, the clips 12 are arranged in groups, two clips 12 form a group, and the clips 12 in each group are symmetrically arranged; the clip 12 is arranged in an arc shape, and the inner side of the clip 12 is embedded with a flexible sponge; the reagent bottles placed can be clamped through the arrangement of the reset spring 11 and the clip 12, and different sizes of reagent bottles can be clamped through the extension and contraction of the reset spring 11.

[0039] In the embodiment 3, the first isolation plate 22 is provided with a placing groove 9, and a spare pressure rod 26 is movably arranged in the placing groove 9; the top end of the pressure rod 21 is provided with a mounting hole, and the spare pressure rod 26 can be inserted into the mounting hole; the bottom surface of the seesaw 17 is provided with a third hinge 19 in the middle, the inside bottom end of the reagent bottle storage cavity 2 is provided with a support column 18, and the bottom end of the third hinge 19 is hinged to the support column 18; the end of the seesaw 17 is provided with a second hinge 16, the seesaw 17 is hinged to the horizontal plate 15 through the second hinge 16, the two ends of the horizontal plate 15 are provided with a first hinge 14, and the bottom end of the jacking block 13 is hinged to the horizontal plate 15 through the first hinge 14; the spare pressure rod 26 can be inserted into the pressure rod 21, the pressure rod 21 can be moved downward by pressing the spare pressure rod 26, and the end of the seesaw 17 away from the pressure rod 21 can be lifted up through the lever principle under the movement of the pressure rod, the jacking block 13 can be moved upward under the action of the first hinge 14 and the second hinge 16, the reagent bottle placed in the storage groove 10 can be jacked out, and the reagent bottle does not need external tools because it is fixed in the storage groove 10 through the combined action of the reset spring 11 and the clip 12.

[0040] In the embodiment 4, the end of the NK cell kit body 1 is hinged to a cover 4, the inner side of the cover 4 is provided with a sealing film 5, and the sealing film 5 is arranged in close contact with the top end of the NK cell kit body 1; the side of the NK cell kit body 1 is provided with an observation window 8, and the observation window 8 leads to the cell culture cavity 3; the inside of the NK cell kit body 1 can be sealed through the arrangement of the sealing film 5, the inside of the cell culture cavity 3 can be observed in real time through the observation window 8, and the cells in the cell culture cavity 3 can be taken out and placed by the dropper 7, and the operation is strong.

[0041] The above merely provides the preferred embodiments of the present application, and is not used to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the principles and technical scope of the present application shall fall into the scope of the present application.

Claims

1. An NK cell in-vitro expansion kit, comprising an NK cell kit body (1), wherein a separation plate is arranged in the middle of the NK cell kit body (1), and a reagent bottle storage cavity (2) and a cell culture cavity (3) are arranged on both sides of the separation plate, characterized in that: a first isolation plate (22) is arranged at the top of the reagent bottle storage cavity (2), at least two storage grooves (10) are arranged on the first isolation plate (22), a limiting cavity (20) is arranged on the first isolation plate (22), a press rod (21) is movably arranged in the limiting cavity (20), a flap (17) is hingedly connected to the bottom end of the press rod (21), a horizontal plate (15) is hingedly connected to the end of the flap (17) away from the press rod (21), and top lifting blocks (13) are hingedly connected to both ends of the horizontal plate (15) and penetrate into the two storage grooves (10) arranged in the longitudinal direction; a second isolation plate (25) is arranged at the top of the cell culture cavity (3), a universal ball (6) is movably arranged in the second isolation plate (25), a dropper (7) is movably arranged on the universal ball (6), a through hole (602) is arranged in the universal ball (6), the dropper (7) is movably arranged in the through hole (602), a plurality of magnetic baffles (601) are symmetrically hingedly arranged in the through hole (602), a plurality of partition plates (24) are arranged in the cell culture cavity (3), and a plurality of temperature controllers (23) are uniformly arranged in the cell culture cavity (3). A placing groove (9) is arranged on the first isolation plate (22), a standby press rod (26) is movably arranged in the placing groove (9), an installation hole is arranged at the top end of the press rod (21), and the standby press rod (26) can be inserted into the installation hole. A third hinge (19) is arranged in the middle of the bottom surface of the flap (17), a support column (18) is arranged at the bottom end of the reagent bottle storage cavity (2), and the bottom end of the third hinge (19) is hingedly connected to the support column (18). A second hinge (16) is arranged at the end of the flap (17), the flap (17) is hingedly connected to the horizontal plate (15) through the second hinge (16), first hinges (14) are arranged at both ends of the horizontal plate (15), and the bottom end of the top lifting block (13) is hingedly connected to the horizontal plate (15) through the first hinge (14). A cover (4) is hingedly connected to the end of the NK cell kit body (1), a sealing film (5) is arranged on the inner side of the cover (4), and the sealing film (5) is arranged in close contact with the top end of the NK cell kit body (1). A hole is arranged at the bottom end of the storage groove (10), and the top lifting block (13) penetrates through the hole.

2. A kit for the in vitro expansion of NK cells according to claim 1, characterized in that, A plurality of reset springs (11) are arranged in abutment with the inner wall of the storage groove (10), a clip (12) is arranged in abutment with the side of the reset spring (11) away from the inner wall of the storage groove (10), the clips (12) are arranged in groups, two clips (12) form a group, and each group of clips (12) is arranged symmetrically.

3. The NK cell ex vivo expansion kit of claim 1, wherein the IL-15 is present in the medium at a concentration of about 10 ng / ml. 5 The clip (12) is arranged in an arc shape, and a flexible sponge is embedded on the inner side of the clip (12).

4. A kit for the in vitro expansion of NK cells according to claim 3, characterized in that, ​ 5. A kit for the in vitro expansion of NK cells according to claim 4, characterized in that, ​ 6. The NK cell ex vivo expansion kit of claim 1, wherein, The side of the NK cell kit body (1) is provided with an observation window (8) opening into the cell culture cavity (3).

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