Transfer device for living beings

Abstract

A biological transfer device for arranging an object in a living body includes a plurality of movable units. Each movable unit includes one or more needle-shaped portions extending in a first direction. Each needle-shaped portion has a cylindrical shape capable of housing the object therein. Each movable unit is movable in the first direction.

Description

Technical Field

[0001] The present disclosure relates to a living body transfer device for transferring an object into a living body. Background Art

[0002] Technologies for transplanting cell populations into living organisms are being developed. For example, attempts have been made to regenerate hair by culturing a cell population that contributes to the formation of hair follicles, the organs responsible for hair growth, and then transplanting this cell population into the skin. To achieve optimal hair regeneration, it is desirable to produce hair follicles with a normal histological structure and excellent hair-forming ability from the transplanted cell population. Therefore, various methods for producing cell populations capable of forming such hair follicles are being researched and developed (for example, see Patent Documents 1 to 3).

[0003] In addition, the development of equipment, i.e., cell transplantation devices, for transferring the cultured cell group from a culture vessel to a living body is also being continuously advanced (e.g., with reference to patent document 4). The cell transplantation device has a needle-shaped cylindrical structure, i.e., a needle-shaped portion, which is tapering to a needle-shaped shape, and takes the cell group from the culture vessel to the inside of the needle-shaped portion. After the needle-shaped portion pierces the target site of transplantation, such as the skin, the cell group is released from the front end of the needle-shaped portion, whereby the cell group is configured in the living body. If the cell transplantation device has multiple needle-shaped portions, multiple cell groups can be transplanted in a concentrated manner, and therefore the efficiency of transplantation can be improved.

[0004] Prior art literature

[0005] Patent Literature

[0006] Patent Document 1: International Publication No. 2017 / 073625

[0007] Patent Document 2: International Publication No. 2012 / 108069

[0008] Patent Document 3: Japanese Patent Application Laid-Open No. 2008-29331

[0009] Patent Document 4: International Publication No. 2019 / 064653 Summary of the Invention

[0010] Problems to be solved by the invention

[0011] However, in order to achieve the effects of tissue regeneration and other effects brought about by cell population transplantation, it is desirable to have a high survival rate of the cell population and to maintain good cell population activity within the organism. The depth at which the cell population is placed within the organism during transplantation is one of the factors that influences the survival rate and activity of the cell population. Therefore, it is preferred to minimize the deviation between the planned cell population placement depth and the actual placement depth.

[0012] However, the more the number of needle-shaped portions that the cell transplantation device has, the greater the pressing force and tensile force that the target part of the transplantation will receive from the cell transplantation device when the needle-shaped portions are inserted and withdrawn. In addition, the more the number of needle-shaped portions that are inserted and withdrawn within a close range, the more the part that each needle-shaped portion inserts will receive the pressing force and tensile force that are generated due to the insertion and withdrawal of other needle-shaped portions around it. The skin and internal organs of the organism have flexibility and elasticity, so the target part will be able to expand and contract due to the above-mentioned pressing force and tensile force. As a result, due to the reasons such as the penetration depth of the needle-shaped portion with respect to the predetermined depth deviation, the actual depth configured by the cell group with respect to the predetermined depth deviation will become larger.

[0013] Such a problem is not limited to cell transplantation devices, but is common to devices for delivering objects such as solid drugs from the surface of tissues to the interior of tissues in a living body.

[0014] An object of the present disclosure is to provide a living body transporting device capable of suppressing variations in the arrangement depth of an object.

[0015] Means for solving problems

[0016] In one embodiment, a biological transfer device for placing an object into a living body is provided. The biological transfer device includes a plurality of movable units. Each movable unit has one or more needle-shaped portions extending along a first direction, each needle-shaped portion having a cylindrical shape capable of accommodating the object. Each movable unit is configured to be movable along the first direction. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a diagram showing a cell transplantation device as an example of a living body transfer device in one embodiment of the living body transfer device.

[0018] Figure 2 This is a diagram showing an example of the structure of a needle-shaped portion included in a cell transplantation device according to one embodiment.

[0019] Figure 3 This is a diagram showing a transplant accommodation step in a transplantation method using a cell transplantation device according to one embodiment.

[0020] Figure 4 This is a diagram showing a transplant accommodation step in a transplantation method using a cell transplantation device according to one embodiment.

[0021] Figure 5 This is a diagram showing a transplant accommodation step in a transplantation method using a cell transplantation device according to one embodiment.

[0022] Figure 6This is a diagram showing the insertion step of the needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0023] Figure 7 This is a diagram showing the insertion step of the needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0024] Figure 8 This is a diagram showing the insertion step of the needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0025] Figure 9 This is a diagram showing the insertion step of the needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0026] Figure 10 This is a diagram showing the insertion step of the needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0027] Figure 11 This is a diagram showing the insertion step of the needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0028] Figure 12 This is a diagram showing the insertion step of the needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0029] Figure 13 This is a diagram showing a transplant placement step in a transplantation method using a cell transplantation device according to one embodiment.

[0030] Figure 14 This is a diagram showing a transplant placement step in a transplantation method using a cell transplantation device according to one embodiment.

[0031] Figure 15 This is a diagram showing the insertion step of the needle-shaped portion in a transplantation method using a conventional cell transplantation device.

[0032] Figure 16 This is a diagram showing a step of removing a needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0033] Figure 17 This is a diagram showing a step of removing a needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0034] Figure 18 This is a diagram showing a step of removing a needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0035] Figure 19This is a diagram showing a step of removing a needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0036] Figure 20 This is a diagram showing a step of removing a needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0037] Figure 21 This is a diagram showing a step of removing a needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0038] Figure 22 This is a diagram showing a step of removing a needle-shaped portion in a transplantation method using a cell transplantation device according to one embodiment.

[0039] Figure 23 This is a diagram showing the step of removing the needle-shaped portion in a transplantation method using a conventional cell transplantation device.

[0040] Figure 24 This is a timing chart showing the transition of movement of the movable unit in the cell transplantation apparatus according to one embodiment. DETAILED DESCRIPTION

[0041] Reference Figures 1 to 24 An embodiment of a living body transfer device will be described. The living body transfer device of this embodiment is embodied as a cell transplantation device used for cell transplantation.

[0042] [Graft]

[0043] The cell transplantation device of this embodiment is used to transplant a transplant into a living body. The transplanted area is at least one of the intradermal and subcutaneous regions, or within tissues such as organs. The transplant comprises a cell population. The cell population that is the target of the transplant will be described below.

[0044] The cell group of the transplanted object comprises a plurality of cells. The cell group can be an aggregate of a plurality of cells that are aggregated, or an aggregate of a plurality of cells that are combined by intercellular binding. Alternatively, the cell group can also be composed of a plurality of dispersed cells. In addition, the cells constituting the cell group can be undifferentiated cells, or cells that have terminated differentiation, and the cell group can also comprise undifferentiated cells and differentiated cells. The cell group is, for example, a cell mass (spheroid), primordium, tissue, organ, etc.

[0045] A cell population, when placed in a target area, possesses the ability to contribute to tissue formation in a living organism. An example of such a cell population is a cell aggregate comprising skin stem cells. The cell population to be transplanted is, for example, placed intradermally or subcutaneously, thereby contributing to hair growth or development. Such a cell population may possess the ability to function as a hair follicle organ, the ability to differentiate into a hair follicle organ, the ability to induce or promote the formation of a hair follicle organ, or the ability to induce or promote hair formation within hair follicles. Furthermore, the cell population may also contain cells that contribute to hair color control, such as pigment cells or stem cells that differentiate into pigment cells.

[0046] Specifically, an example of the cell population to be transplanted in this embodiment is the hair follicle primordium, which serves as the primary hair follicle organ. The hair follicle primordium comprises mesenchymal cells and epithelial cells. Within the hair follicle organ, dermal papilla cells, which serve as mesenchymal cells, induce the differentiation of hair follicle epithelial stem cells. Through the resulting hair bulb, hair matrix cells repeatedly divide, forming hair. The hair follicle primordium is the cell population that differentiates into this hair follicle organ.

[0047] The hair follicle primordium is formed, for example, by co-culturing mesenchymal cells derived from mesenchymal tissues such as the dermal papilla and epithelial cells derived from epithelial tissues located in the bulge region or the base of the hair bulb under predetermined conditions. However, the method for producing the hair follicle primordium is not limited to the above example. Furthermore, the sources of the mesenchymal and epithelial cells used to produce the hair follicle primordium are also not limited; these cells may be derived from the hair follicle organ, from an organ other than the hair follicle organ, or from pluripotent stem cells.

[0048] Furthermore, the transplant may include, together with the cell population, a member that assists the transplantation of the cell population.

[0049] [Cell transplantation device]

[0050] like Figure 1 As shown, the cell transplantation apparatus 100 includes a plurality of movable units 10 and an outer peripheral portion 30 surrounding the plurality of movable units 10 .

[0051] Each movable unit 10 includes one or more needle-like portions 21 and a support portion 20 that supports the needle-like portions 21. The needle-like portions 21 have a cylindrical shape extending in one direction, in other words, a hollow needle shape. The outer shape of the needle-like portion 21 is not particularly limited, as long as its tip portion is shaped to penetrate the target site for transplantation, such as the skin of a living organism. For example, the tip portion of the needle-like portion 21 may be formed by cutting a cylinder obliquely relative to its extension direction, and may taper.

[0052] The support portion 20 surrounds the needle-shaped portion 21 except for the portion near the front end thereof, thereby supporting the needle-shaped portion 21. In other words, the portion near the front end of the needle-shaped portion 21 protrudes from the front end surface of the support portion 20 along the extension direction of the needle-shaped portion 21. For example, the needle-shaped portion 21 is assembled on the support portion 20 by passing it through a hole provided in the support portion 20. When the movable unit 10 includes a plurality of needle-shaped portions 21, the support portion 20 supports the plurality of needle-shaped portions 21 collectively. The plurality of needle-shaped portions 21 are supported by one support portion 20, whereby the plurality of needle-shaped portions 21 and the support portion 20 become one body, thereby constituting the movable unit 10.

[0053] The movable unit 10 is configured to be movable along the extending direction of the needle-shaped portion 21. The materials of the needle-shaped portion 21 and the support portion 20 are not particularly limited, and they may be formed of, for example, resin or metal.

[0054] The number of movable units 10 included in the cell transplantation device 100 is not particularly limited, as long as it is two or more. The peripheral portion 30 surrounds the outer surface of the assembly of movable units 10. The arrangement of the plurality of movable units 10 is not particularly limited. The plurality of movable units 10 may be arranged regularly, for example, in a two-dimensional grid pattern such as a square grid or a regular hexagonal grid, in concentric circles, in a straight line, or in an irregular arrangement. Furthermore, the front end surfaces of the support portions 20 of adjacent movable units 10 may be in contact with or separated from each other.

[0055] In the plurality of movable units 10, the number and arrangement of the needle-like portions 21 of each movable unit 10 may be the same or different. In addition, in the state where the plurality of movable units 10 are arranged, in other words, in the aggregate composed of the plurality of movable units 10, the plurality of needle-like portions 21 of the cell transplantation device 100 may be arranged regularly or irregularly. Figure 1 The figure illustrates a cell transplantation device 100 comprising six movable units 10, each of which has two needle-like portions 21. The six movable units 10 are arranged in order from the left side of the drawing, namely, movable unit 10a, movable unit 10b, movable unit 10c, movable unit 10d, movable unit 10e, and movable unit 10f. Within the assembly of six movable units 10, the needle-like portions 21 are arranged at predetermined intervals.

[0056] In order to improve the efficiency of transplantation, the total number of needle-shaped parts 21 provided by the plurality of movable units 10, that is, the total number of needle-shaped parts 21 provided by the cell transplantation device 100, is preferably 5 or more. In addition, in the cell transplantation device 100, the needle-shaped parts 21 are preferably arranged at a ratio of 1 cm 2 The density of the needle-shaped portions 21 is 5 or more. In addition, the number of needle-shaped portions 21 included in one movable unit 10 is preferably 10 or less.

[0057] The cell transplantation apparatus 100 further includes a drive unit 40, a suction and pressurizing unit 41, and a control unit 42. The drive unit 40 moves each of the plurality of movable units 10 along the extending direction of the needle-shaped portion 21. Specifically, the drive unit 40 moves the movable unit 10 relative to the outer peripheral portion 30 between a position in which the tip of the needle-shaped portion 21 of the movable unit 10 is located within the space enclosed by the outer peripheral portion 30 and a position in which the tip of the needle-shaped portion 21 of the movable unit 10 extends from the space. In other words, the drive unit 40 moves the movable unit 10 between a position in which the tip of the needle-shaped portion 21 does not protrude from the tip of the outer peripheral portion 30 and a position in which the tip of the needle-shaped portion 21 protrudes from the tip of the outer peripheral portion 30.

[0058] The driving unit 40 includes electronic components such as a motor, and moves the plurality of movable units 10 independently of each other, that is, moves each movable unit 10 individually, based on a control signal from the control unit 42 .

[0059] The suction and pressurizing unit 41 assists the introduction and release of the graft into and out of the needle-shaped portion 21. The suction and pressurizing unit 41 introduces the graft into the needle-shaped portion 21 by suctioning the interior of the needle-shaped portion 21, and releases the graft from the needle-shaped portion 21 by pressurizing the interior of the needle-shaped portion 21.

[0060] The suction and pressurizing unit 41 suctions and pressurizes the needle-shaped portion 21 of each movable unit 10 in response to control signals from the control unit 42. The suction and pressurizing unit 41 can suction and pressurize the needle-shaped portion 21 of each movable unit 10 individually, or it can suction and pressurize the needle-shaped portions 21 of multiple movable units 10 simultaneously. The suction and pressurizing unit 41 includes a mechanism such as a syringe and a pump that enables suction and pressurization, and is connected to each movable unit 10 in a manner that enables suction and pressurization within the needle-shaped portion 21.

[0061] The control unit 42 controls the movement of the movable unit 10. Specifically, the control unit 42 controls the timing of the driving unit 40 to move each movable unit 10. In addition, the control unit 42 controls the timing of the suction and pressurization of the needle-shaped portion 21 of each movable unit 10 by the suction and pressurization unit 41, that is, the timing of the introduction and release of the transplant into the needle-shaped portion 21. The control unit 42 includes a control circuit and a memory that generate control signals for these controls. Specifically, the control unit 42 may also have dedicated hardware that performs at least a part of the various processes, namely, an application-specific integrated circuit (ASIC). The control unit 42 may also be composed of a circuit including one or more dedicated hardware circuits such as ASICs, one or more processors that operate according to software as a computer program, namely, microcomputers, or a combination of these.

[0062] Reference Figure 2An example of the detailed structure of the needle-shaped portion 21 will be described. The needle-shaped portion 21 includes a first tube 22 including the front end portion of the needle-shaped portion 21, and a second tube 23 having a flow path cross-sectional area larger than that of the first tube 22. The first tube 22 and the second tube 23 are each cylindrical with a certain inner diameter and extend along the extension direction of the needle-shaped portion 21. The front end portion of the first tube 22 forms the front end portion of the needle-shaped portion 21 and has an opening portion 25. The second tube 23 is connected to the base end portion of the first tube 22. The internal space of the first tube 22 and the internal space of the second tube 23 are connected to each other, forming a flow path.

[0063] The needle-shaped portion 21 has a barrier 24 near the location where the cross-sectional area of ​​the flow path changes. The barrier 24 intersects the flow path midway within the needle-shaped portion 21. The barrier 24 allows liquid to pass through the barrier 24 from the distal end to the proximal end of the needle-shaped portion 21, thereby inhibiting the passage of the graft.

[0064] The barrier portion 24 comprises, for example, a plurality of fibers arranged in a grid pattern, and covers the proximal end portion of the first tube 22. The proximal end portion of the first tube 22 covered by the barrier portion 24 is then inserted into the distal end portion of the second tube 23. The number, material, and arrangement of the fibers in the barrier portion 24 are not particularly limited as long as the passage of the graft can be inhibited.

[0065] In addition, the needle-shaped portion 21 is not limited to Figure 2 The structure shown here only needs to have a cylindrical structure that allows the graft to be introduced into the needle-shaped portion 21 through the opening 25 at the front end and accommodates the graft within the needle-shaped portion 21. However, to improve the efficiency of transplantation, the needle-shaped portion 21 is preferably configured so that the introduced graft is retained near the front end within the needle-shaped portion 21, as in the case of the stopper 24. Furthermore, the needle-shaped portion 21 and the support portion 20 may be formed integrally.

[0066] [Transplantation method]

[0067] A transplantation method of a transplant using the cell transplantation apparatus 100 will be described.

[0068] First, the procedure for receiving the transplant is described. Figure 3 As shown in FIG. 1 , before transplantation, the graft Cg is held together with the protective solution P1 by a tray 50 such as a culture container. Figure 3 As shown, the graft Cg and the protective solution P1 are placed in the recess 51 of the tray 50. The method of holding the graft Cg and the protective solution P1 in the tray 50 is not limited to holding the graft Cg and the protective solution P1 in the recess 51. For example, the graft Cg and the protective solution P1 may be placed on a flat surface of the tray 50. Furthermore, the tray 50 may be a container different from the culture container, and the graft Cg may be transferred from the culture container to the tray 50.

[0069] The protective liquid P1 can be any liquid that does not easily hinder the survival of cells, and preferably has little impact on the organism when injected into the organism. For example, the protective liquid P1 is a liquid that protects the skin, such as physiological saline, vaseline, or lotion, or a mixture of these liquids. The protective liquid P1 may also contain additives such as nutrients. In addition, when the tray 50 is a culture container and the transplant Cg is cultured in the tray 50, the protective liquid P1 can be a culture medium for cell culture or a liquid replaced from the culture medium. The liquid containing the transplant Cg and the protective liquid P1 can be a low-viscosity fluid or a high-viscosity fluid.

[0070] When the graft Cg is taken in, the tip of the needle-shaped portion 21 faces the portion of the tray 50 where the graft Cg and the protective solution P1 are located. Then, the graft Cg and the protective solution P1 are taken in from the opening 25 of the needle-shaped portion 21 into the interior of the needle-shaped portion 21. For example, Figure 3 As shown, after the recess 51 of the tray 50 is aligned with the position of the needle portion 21, as shown in FIG. Figure 4 As shown, the front end of the needle portion 21 is placed in the recess 51. Figure 5 As shown, by the operation of the suction and pressurizing portion 41, the graft Cg is sucked into the interior of the needle-shaped portion 21 through the opening 25 together with the protective liquid P1.

[0071] Here, the graft Cg, which has entered the needle-shaped portion 21 through the opening 25, moves toward the barrier 24 within the flow path of the needle-shaped portion 21 as the protective liquid P1 flows. The barrier 24 allows the protective liquid P1 to pass through, so the protective liquid P1 flows within the needle-shaped portion 21 toward the proximal end as suction is applied. On the other hand, the barrier 24 does not allow the graft Cg to pass through, so the graft Cg remains distally of the barrier 24. Thus, the graft Cg is accommodated near the distal end of the needle-shaped portion 21. The protective liquid P1 surrounds the graft Cg. After the graft Cg is taken in, suction is stopped.

[0072] The graft Cg is preferably introduced collectively for all of the needle-like sections 21 included in the cell transplantation apparatus 100. Specifically, each needle-like section 21 preferably simultaneously faces the graft Cg on the tray 50, and each needle-like section 21 performs suction at the same timing, thereby introducing the graft Cg into each needle-like section 21. This improves the efficiency of introducing the graft Cg. Furthermore, the graft Cg can be introduced with or without the tip of the needle-like section 21 protruding from the tip of the peripheral section 30. Aligning the tip of each needle-like section 21 facilitates collective introduction of the graft Cg.

[0073] Next, the process of inserting the needle-shaped portion 21 into the target site for transplantation will be described. Figure 6 As shown, the position of each movable unit 10 is such that the tip of the needle-shaped portion 21 is located within the space enclosed by the outer peripheral portion 30, and the cell transplantation device 100 abuts the target site Sk of the transplantation, such as the skin of a living body. Specifically, the cell transplantation device 100 abuts the target site Sk with the tip of the outer peripheral portion 30 protruding beyond the tip of all the needle-shaped portions 21. As a result, the tip of the outer peripheral portion 30 contacts the surface of the target site Sk, and the needle-shaped portions 21 are not inserted into the target site Sk.

[0074] Next, by the operation of the driving unit 40, one of the plurality of movable units 10 moves so that the tip of the needle-shaped portion 21 of the movable unit 10 protrudes from the outer peripheral portion 30. That is, the one movable unit 10 moves toward the surface of the target site Sk, whereby the needle-shaped portion 21 of the movable unit 10 penetrates the target site Sk. Figure 7 The figure shows a state in which the movable unit 10 a located on the leftmost side of the paper among the six movable units 10 moves and the needle-shaped portion 21 of the movable unit 10 a penetrates the target site Sk.

[0075] Similarly, the plurality of movable units 10 are moved one at a time, and the needle-shaped portion 21 sequentially penetrates the target site Sk according to each movable unit 10. For example, in the figure, the movable units 10 are moved one at a time from left to right facing the paper. That is, the movable unit 10a is moved next, as shown in FIG. Figure 8 As shown in FIG. 1 , the movable unit 10b adjacent to the movable unit 10a moves, and the needle-like portion 21 of the movable unit 10b penetrates the target site Sk. Figure 9 、 Figure 10 、 Figure 11 、 Figure 12 As shown in the sequence, the movable units 10 are moved in the order of the movable unit 10c, the movable unit 10d, the movable unit 10e, and the movable unit 10f, and the needle-shaped portion 21 of each movable unit 10 penetrates the target site Sk. Figure 12 As shown, the last movable unit 10f moves and the needle-like portion 21 of the movable unit 10f penetrates the target site Sk, thereby forming a state in which the tip ends of the needle-like portions 21 of all movable units 10 protrude from the tip end of the outer peripheral portion 30, and all the needle-like portions 21 of the cell transplantation device 100 penetrate the target site Sk.

[0076] In this manner, the length of each needle-shaped portion 21 inserted into the target site Sk is defined as the length of the portion of each needle-shaped portion 21 protruding from the distal end surface, with the distal end surface of the outer peripheral portion 30 as a reference.

[0077] When all the needle-like parts 21 of the cell transplantation device 100 have penetrated the target site Sk, the process of placing the graft Cg is performed. This process of placing the graft Cg is described below. Figure 13 As shown, when the needle-shaped portion 21 containing the graft Cg is inserted into the target site Sk, the inside of each needle-shaped portion 21 is pressurized by the operation of the suction pressurizing portion 41. As a result, Figure 14 As shown, the graft Cg housed in the needle-shaped portion 21 is pushed out together with the protective liquid P1 and discharged from the opening 25. Thus, the graft Cg is arranged inside the target site Sk, that is, in the transplantation target region.

[0078] The graft Cg is preferably released from the needle-shaped portion 21 collectively for all the needle-shaped portions 21 included in the cell transplantation apparatus 100. That is, each needle-shaped portion 21 is preferably pressurized at the same timing, thereby releasing the graft Cg from each needle-shaped portion 21. This improves the efficiency of disposing the graft Cg to the target area for transplantation.

[0079] Here, the function of the cell transplantation device 100 of this embodiment will be described by comparing it with the entry process and the placement process of a conventional cell transplantation device. Figure 15 As shown, conventional cell transplantation devices 110 lack independently movable units, and all needle-like sections 21 penetrate the target site Sk simultaneously. As a result, the load applied simultaneously by the cell transplantation device 110 to the target site Sk increases. Furthermore, because a large number of needle-like sections 21 penetrate the target site Sk within a close proximity, the portion penetrated by each needle-like section 21 experiences tensile forces from surrounding areas caused by the penetration of other needle-like sections 21. Consequently, the target site Sk tends to stretch and sag. Consequently, the penetration depth of the needle-like sections 21 tends to deviate from the intended depth.

[0080] Because the target site Sk of a living body does not stretch uniformly, the degree of depression of the target site Sk varies depending on the position at which the needle-shaped portion 21 is inserted. Consequently, the depth of insertion of the needle-shaped portion 21 varies from needle-shaped portion 21 to needle-shaped portion 21, making it difficult to correct the depth of insertion of the needle-shaped portion 21 using the conventional cell transplantation device 110. When the depth of insertion of the needle-shaped portion 21 varies, the depth at which the graft Cg is released from the tip of the needle-shaped portion 21 also deviates from the intended depth.

[0081] In contrast, in the cell transplantation device 100 of this embodiment, multiple needle-like portions 21 are distributed across multiple movable units 10. The needle-like portions 21 of each movable unit 10 penetrate the target site Sk at different timings. Therefore, compared to conventional methods, the number of needle-like portions 21 that penetrate the target site Sk at a time is reduced. As a result, the load applied to the target site Sk by the cell transplantation device 100 is reduced, and the tensile force exerted on the surrounding area by the portion penetrated by each needle-like portion 21 is also reduced. This prevents the target site Sk from sinking, thereby preventing the penetration depth of the needle-like portion 21 from deviating from the predetermined depth. In other words, the depth at which the graft Cg is positioned can be prevented from deviating from the predetermined depth. Furthermore, since deformation of the target site Sk can be suppressed, the position of the graft Cg along the surface of the target site Sk can also be prevented from deviating from the predetermined position.

[0082] Furthermore, since variations in the insertion depths of the needle-shaped portions 21 can be suppressed among the plurality of needle-shaped portions 21 , variations in the placement depths of the plurality of grafts Cg can be suppressed when the grafts Cg are to be placed at the same depth using the plurality of needle-shaped portions 21 .

[0083] After the placement step, the extraction step of extracting the needle-shaped portion 21 from the target site Sk is performed. This extraction step will be described. First, the drive unit 40 operates to move one of the multiple movable units 10 in a direction away from the surface of the target site Sk. This causes the needle-shaped portion 21 of the movable unit 10 to be extracted from the target site Sk. The movable unit 10 moves to a position where the tip of the needle-shaped portion 21 enters the space surrounded by the outer peripheral portion 30. Figure 16 The figure shows a state in which the movable unit 10a, which is located on the leftmost side of the drawing, among the six movable units 10, moves and the needle portion 21 of the movable unit 10a is pulled out from the target site Sk. The graft Cg is arranged inside the target site Sk.

[0084] Similarly, the plurality of movable units 10 are moved one at a time, and the needle-shaped portion 21 is sequentially pulled out from the target site Sk according to each movable unit 10. For example, in the figure, the movable units 10 are moved one at a time from left to right facing the paper. That is, after the movable unit 10a, as shown in FIG. Figure 17 As shown in FIG, the movable unit 10b adjacent to the movable unit 10a moves, and the needle-shaped portion 21 of the movable unit 10b is pulled out from the target site Sk. Figure 18 、 Figure 19 、 Figure 20 、 Figure 21As shown in the sequence, the movable units 10 are moved in the order of the movable unit 10c, the movable unit 10d, the movable unit 10e, and the movable unit 10f, and the needle-shaped portion 21 of each movable unit 10 is pulled out from the target site Sk. Figure 21 As shown, the last movable unit 10f moves, and the needle-like portion 21 of the movable unit 10f is pulled out from the target site Sk. This results in all the needle-like portions 21 of the cell transplantation apparatus 100 being pulled out from the target site Sk. At this point, the tips of the needle-like portions 21 of all movable units 10 do not protrude from the tip of the outer peripheral portion 30.

[0085] Therefore, if Figure 22 As shown, the graft Cg remains inside the target site Sk, and the transplantation of the graft Cg is completed.

[0086] Here, the function of the cell transplantation device 100 of this embodiment will be described by comparing it with the extraction process of a conventional cell transplantation device. Figure 23 As shown, conventional cell transplantation devices 110 lack independently movable units, so all needle-like sections 21 are simultaneously withdrawn from the target site Sk. Consequently, the tensile force applied simultaneously by the cell transplantation device 110 to the target site Sk increases. Furthermore, because many needle-like sections 21 are withdrawn from the target site Sk within a close proximity, the portion of the target site Sk where each needle-like section 21 is inserted receives tensile force from surrounding areas caused by the withdrawal of other needle-like sections 21. Consequently, the target site Sk tends to stretch, being pulled up along with the cell transplantation device 110.

[0087] When the target site Sk is pulled up after the graft Cg is positioned, the graft Cg moves within the target site Sk, and the depth of the graft Cg tends to deviate from the intended placement depth of the graft Cg. The degree of extension of the target site Sk varies depending on the position at which the needle-shaped portion 21 is inserted. Therefore, with the conventional cell transplantation apparatus 110, it is difficult to correct for the deviation in the depth of the graft Cg.

[0088] In contrast, in the cell transplantation device 100 of this embodiment, the needle-like portions 21 of the multiple movable units 10 are withdrawn from the target site Sk at different timings. Therefore, compared to conventional methods, the number of needle-like portions 21 withdrawn from the target site Sk at a time is reduced. As a result, the tensile force applied to the target site Sk by the cell transplantation device 100 is reduced. Furthermore, the tensile force exerted on the surrounding area by the portion into which each needle-like portion 21 is inserted is also reduced. This prevents the target site Sk from being pulled up, thereby preventing the deployed graft Cg from moving and causing its depth to deviate from the intended deployment depth. Furthermore, since deformation of the target site Sk can be suppressed, positional deviation of the graft Cg along the surface of the target site Sk can also be suppressed.

[0089] Figure 24 An example of the timing of movement of each movable unit 10 and release of the graft Cg is shown. Such timing is controlled by the control unit 42.

[0090] Figure 24 The solid line in the figure represents the displacement of the tip position of the needle portion 21 of each movable unit 10 in the depth direction of the target site Sk, where min represents the position opposite the surface of the target site Sk, and max represents the deepest insertion position into the target site Sk. When the tip of the needle portion 21 is at the min position, the tip of the needle portion 21 does not protrude from the tip of the outer peripheral portion 30 and does not penetrate the target site Sk. When the tip of the needle portion 21 is at the max position, the tip of the needle portion 21 protrudes from the tip of the outer peripheral portion 30 and penetrates the target site Sk. The amount of movement of the movable unit 10 and the length of the needle portion 21 are set so that the graft Cg is deployed at the desired depth by the needle portion 21 at the max position.

[0091] like Figure 24 As shown, when the cell transplantation device 100 is positioned on the target site Sk, at timing t1, the movable unit 10a begins moving, and the needle-shaped portion 21 of the movable unit 10a begins to enter the target site Sk. At timing t2, when the needle-shaped portion 21 of the movable unit 10a reaches the maximum position, the movement of the movable unit 10a stops, and the movable unit 10b begins moving, and the needle-shaped portion 21 of the movable unit 10b begins to enter the target site Sk. Similarly, at the timing when the needle-shaped portion 21 of the moving movable unit 10 enters the maximum position and the movement of the movable unit 10 stops, the adjacent movable unit 10 begins moving. That is, at timing t3, the movable unit 10c, at timing t4, the movable unit 10d, at timing t5, the movable unit 10e, and at timing t6, the movable unit 10f begin moving at the same time as the adjacent movable unit 10 stops.

[0092] Thus, at timing t7, when the needle-shaped portion 21 of the movable unit 10f reaches its maximum position, the needle-shaped portions 21 of all movable units 10 have completed their entry into the target site Sk. Next, at timing t8, pressurization is applied to the interior of each needle-shaped portion 21, thereby releasing the graft Cg from each needle-shaped portion 21 and placing the graft Cg within the target site Sk.

[0093] Then, at timing t9, movable unit 10a begins to move, and its needle-like portion 21 begins to be pulled out from target site Sk. At timing t10, when the needle-like portion 21 of movable unit 10a is pulled up to the min position, the movement of movable unit 10a stops, while movable unit 10b begins to move, and the needle-like portion 21 of movable unit 10b begins to be pulled out. Similarly, at the timing when the needle-like portion 21 of a moving movable unit 10 is pulled up to the min position and the movement of that movable unit 10 stops, the adjacent movable unit 10 begins to move. That is, at timing t11, movable unit 10c begins to move, at timing t12, movable unit 10d begins to move, at timing t13, movable unit 10e begins to move, and at timing t14, movable unit 10f begins to move simultaneously with the stoppage of the adjacent movable unit 10.

[0094] At timing t15 when the needle-shaped portion 21 of the movable unit 10 f is pulled up to the min position, all the movable units 10 are in a state where the needle-shaped portion 21 has been completely removed from the target site Sk, and the transplantation of the graft Cg is completed.

[0095] As described above, since the next movable unit 10 starts moving at the same time as one movable unit 10 stops moving, the time required to move all movable units 10 can be shortened compared to a case where one movable unit 10 stops moving and then the next movable unit 10 starts moving after a certain interval. This improves transplantation efficiency.

[0096] Furthermore, after the insertion process of the needle-shaped portions 21 of all movable units 10 is completed, the placement process is performed on all the needle-shaped portions 21 of the movable units 10 at once. After this process is completed, the removal process of the needle-shaped portions 21 of each movable unit 10 is performed. Therefore, the process performed by the cell transplantation apparatus 100 is always one of the following: insertion, placement, or removal. Different processes are not mixed simultaneously. For example, the insertion process of one movable unit 10 and the placement process of another movable unit 10 are not performed simultaneously. Therefore, the operation of the cell transplantation apparatus 100 can be prevented from becoming complicated, and the stability of the execution of each process is improved. Furthermore, for example, after the insertion process and the placement process of all the needle-shaped portions 21, it is easy to add a confirmation process to confirm whether these processes have been reliably completed.

[0097] In addition, the timing of the movement of the plurality of movable units 10 is not limited to the above-mentioned examples. In the above, an example is given in which the plurality of movable units 10 move sequentially from the end, but the order of movement of the movable units 10 is not particularly limited. For example, in the case where the plurality of movable units 10 are arranged two-dimensionally in a concentric circle or a grid, the movable units 10 may also move in the order from the center portion toward the edge portion of the arrangement, or from the edge portion toward the center portion. Alternatively, the order in which the movable units 10 move may be random relative to the arrangement of the movable units 10. In addition, the order in which the movable units 10 move may also be different in the entry process and the extraction process of the needle-shaped portion 21.

[0098] In each of the advancing step and the withdrawing step, the next movable unit 10 may start moving after a certain interval has elapsed since the movement of one movable unit 10 is completed, or the next movable unit 10 may start moving before the movement of one movable unit 10 is completed.

[0099] Furthermore, the movement timing of all movable units 10 may be different from one another. In other words, the multiple movable units 10 do not need to be moved one at a time. As long as the movement timing of some of the multiple movable units 10 is different from that of the others, this can reduce variations in the placement depth of the graft Cg compared to conventional cell transplantation devices that have the same number of needle-like sections 21 as the cell transplantation device 100 of this embodiment, where all needle-like sections 21 are inserted and removed from the target site Sk simultaneously.

[0100] When some of the multiple movable units 10 move simultaneously, the further the simultaneously moving movable units 10 move away from each other, the smaller the tensile force exerted on the portion of the target site Sk where each needle-shaped portion 21 has penetrated from the surrounding area. Consequently, the variation in the placement depth of the graft Cg decreases.

[0101] From this perspective, it is preferable that adjacent movable units 10 move at different timings. Furthermore, within movable units 10 moving at the same timing, the closest needle-like portions 21 are preferably separated by at least 4 mm. Alternatively, if multiple needle-like portions 21 are regularly arranged within a movable unit 10, the distance between the closest needle-like portions 21 within the movable units 10 moving at the same timing is preferably at least three times the spacing between the needle-like portions 21 within the movable unit 10.

[0102] In addition, the cell transplantation device 100 may also be configured to simultaneously implement different processes among the entry process, configuration process, and extraction process for different movable units 10. For example, the entry process, configuration process, and extraction process may be continuously performed for each movable unit 10, and the start timing of this series of processes may also be different for each movable unit 10. That is, the entry process may be started sequentially for a plurality of movable units 10, and the configuration process may be sequentially performed from the movable unit 10 at which the entry process is completed, and the extraction process may be sequentially performed from the movable unit 10 at which the configuration process is completed. According to the method of continuously performing the entry process, configuration process, and extraction process for each movable unit 10, the above method is different from the above method. Figure 24 Compared with the method of performing one step at a time as shown, the time required to complete the transplantation of all the grafts Cg can be shortened.

[0103] As described above, according to the above-described embodiment, the advantages listed below can be obtained.

[0104] (1) The plurality of movable units 10 are configured so as to be movable along the extension direction of the needle-shaped portion 21. Therefore, the timing of the needle-shaped portion 21 being inserted into the living body and the timing of the needle-shaped portion 21 being withdrawn from the living body can be made different among the plurality of needle-shaped portions 21 included in the cell transplantation device 100. That is, the timing of the insertion into the living body and the timing of the withdrawal from the living body of one needle-shaped portion 21 can be different from those of the other needle-shaped portions 21. Therefore, compared with the case where all the needle-shaped portions 21 are inserted into and withdrawn from the living body at the same time, the force to be applied to the transplanted part Sk in the living body from the cell transplantation device 100 at one time is reduced. In addition, the force to be applied to the part inserted by each needle-shaped portion 21 due to the insertion and withdrawal of the surrounding needle-shaped portions is also reduced. This can suppress the expansion and contraction of the target part, thereby suppressing the deviation of the arrangement depth of the graft Cg from the predetermined depth.

[0105] (2) The control section 42 makes the moving timing of the movable units 10 that are adjacent to each other different from each other when moving the movable units 10 toward the living body in order to make the needle-like sections 21 enter the living body. That is, each movable unit 10 stops at a timing different from the stop of the movement of the other movable units 10 adjacent to the movable unit 10. Therefore, the number of needle-like sections 21 that are simultaneously inserted into the target site Sk is reduced compared to the case where all the needle-like sections 21 are simultaneously inserted into the living body. As a result, the load that the target site Sk receives from the cell transplantation device 100 at one time is reduced. Further, the moving timing of the movable units 10 that are adjacent to each other is different, so the number of needle-like sections 21 that are simultaneously inserted into the target site Sk in the vicinity is reduced. Therefore, the tension that the portion into which each needle-like section 21 is inserted receives from the surroundings thereof is reliably reduced. Thus, the target site Sk can be inhibited from being elongated and depressed, so the depth of the needle-like sections 21 can be inhibited from deviating from the predetermined depth, that is, the depth at which the graft Cg is disposed can be inhibited from deviating from the predetermined depth.

[0106] (3) The control section 42 makes the start timing of the movement of the movable units 10 that are adjacent to each other different from each other when moving the movable units 10 away from the living body in order to make the needle-like sections 21 exit from the living body. That is, each movable unit 10 starts to move at a timing different from the start of the movement of the other movable units 10 adjacent to the movable unit 10. Therefore, the number of needle-like sections 21 that are simultaneously extracted from the target site Sk is reduced compared to the case where all the needle-like sections 21 are simultaneously extracted from the living body. As a result, the tension that the target site Sk receives from the cell transplantation device 100 at one time is reduced. Further, since the moving timing of the movable units 10 that are adjacent to each other is different, the number of needle-like sections 21 that are simultaneously extracted from the target site Sk in the vicinity is reduced. Therefore, the tension that the portion into which each needle-like section 21 is inserted receives from the surroundings thereof is reliably reduced. Thus, the target site Sk can be inhibited from being elongated and pulled up, so the graft Cg disposed in the target site Sk can be inhibited from moving and deviating from the predetermined depth.

[0107] (4) The control unit 42 is configured so that, when the movable unit 10 is moved toward the living body in order to allow the needle-shaped portion 21 to enter the living body, the timing at which the movement of each movable unit 10 stops is different from that of the other movable units 10. That is, each movable unit 10 stops at a timing different from that at which the movement of the other movable units 10 stops. Since the multiple movable units 10 are moved one at a time, the number of needle-shaped portions 21 that are simultaneously inserted into the target site Sk is further reduced. As a result, the load that the target site Sk receives from the cell transplantation device 100 at one time is further reduced, and the tensile force that the portion into which each needle-shaped portion 21 is inserted receives from its surroundings is also reduced. Therefore, the target site Sk can be further suppressed from stretching and sinking, and thus the deviation in the depth of insertion of the needle-shaped portion 21 can be further suppressed, that is, the deviation in the depth of placement of the graft Cg can be further suppressed.

[0108] (5) The control unit 42 is configured so that, when the movable units 10 are moved in order to extract the needle-shaped portion 21 from the living body, the timing of the start of movement of each movable unit 10 is different from that of the other movable units 10. That is, each movable unit 10 starts moving at a timing different from that of the other movable units 10. Since the multiple movable units 10 are moved one at a time, the number of needle-shaped portions 21 that are simultaneously extracted from the target site Sk is further reduced. As a result, the tensile force that the target site Sk receives from the cell transplantation device 100 at one time is further reduced, and the tensile force that the portion into which each needle-shaped portion 21 is inserted receives from its surroundings is also reduced. Therefore, it is possible to further suppress the target site Sk from stretching and being pulled up, and thus it is possible to further suppress the occurrence of deviations in the depth at which the graft Cg is arranged.

[0109] (6) The control unit 42 controls the timing of suction by the suction and pressurizing unit 41, thereby releasing the graft Cg from each needle-shaped portion 21 after the needle-shaped portions 21 of all movable units 10 have completed entry into the living body. Therefore, in the cell transplantation apparatus 100, it is possible to prevent different processes from being mixed and performed simultaneously. This prevents the operation of the cell transplantation apparatus 100 from becoming complicated, and improves the stability of the execution of each process.

[0110] (7) The control unit 42 controls the drive unit 40 and the suction and pressurizing unit 41, thereby continuously performing the insertion of the needle-shaped portion 21 into the living body, the release of the graft Cg from the needle-shaped portion 21, and the removal of the needle-shaped portion 21 from the living body for each movable unit 10. Therefore, compared with the method of performing one step at a time in the cell transplantation apparatus 100, the time required to complete the transplantation of all grafts Cg can be shortened.

[0111] (8) The total number of needle-shaped portions 21 included in the plurality of movable units 10 is 5 or more, and the number of needle-shaped portions 21 in the cell transplantation apparatus 100 is 1 cm 2The needle-like portions 21 are arranged in proximity, and the needle-like portions 21 are arranged divided in the plurality of movable units 10, and thus it is easy to obtain the above-described advantages by controlling the timing of the insertion and extraction of the needle-like portions 21 with respect to the living body.

[0112] (9) When the graft Cg is a cell aggregate including skin stem cells, it is possible to suppress a deviation of a depth at which the cell group is arranged with respect to a predetermined depth when the cell group is transplanted to a skin region. The skin region is formed of a plurality of layers, and the depth at which the cell group is arranged is closely related to the function implementation of the cell group. Thus, by using the cell transplantation device 100 for the transplantation of the cell group to the skin region, it is easy to reliably exert the effects of the regeneration of tissue or the like brought about by the transplantation of the cell group. Further, in a case where the transplantation of the cell group is for the purpose of hair growth or hair development, there are many cases where it is required to transplant a large number of cell groups. By using the cell transplantation device 100 in such transplantation of the cell group, the efficiency of the cell transplantation can be greatly improved, and the effects of hair growth or hair development can be favorably obtained by the function implementation of the cell group.

[0113] [Modified Example]

[0114] The above-described embodiment can be implemented as follows.

[0115] • The moving timing of the plurality of movable units 10 can be different in at least one of the insertion process and the extraction process. That is, in either of the insertion process and the extraction process, the needle-like portions 21 provided in the cell transplantation device 100 can also be moved simultaneously with respect to the target site. If the moving timing of the plurality of movable units 10 is different in at least one of the insertion process and the extraction process, it is possible to suppress a deviation of the arrangement depth of the graft Cg compared to the conventional cell transplantation device in which all of the needle-like portions 21 are inserted and extracted simultaneously in both the insertion process and the extraction process.

[0116] • In the plurality of movable units 10, the moving amount of the movable unit 10 and the length of the needle-like portion 21 can also not necessarily be the same. For example, in a case where the surface of the target site is curved like a scalp, and thus the distance from the initial position of the needle-like portion 21 to the predetermined depth at which the graft Cg is arranged is not necessarily the same in the plurality of needle-like portions 21, it is also possible to make each of the needle-like portions 21 enter the predetermined depth at which the graft Cg is arranged by making the moving amount of a part of the movable units 10 different from the other movable units 10. Also, the predetermined depth at which the graft Cg is arranged, that is, the predetermined depth at which the needle-like portion 21 is inserted can also not necessarily be the same in the plurality of needle-like portions 21.

[0117] The outer peripheral portion 30 does not need to surround the entire outer periphery of the plurality of movable units 10. The outer peripheral portion 30 may be configured to define a reference position when the movable unit 10 moves, for example, by contacting a target portion.

[0118] The cell population to be transplanted need not necessarily be a cell population that contributes to hair growth or development; any cell population that exerts a desired effect when placed in a living body may be sufficient. For example, the cell population to be transplanted may be a cell population that exerts a cosmetic effect, such as eliminating wrinkles or improving moisturizing of the skin. Furthermore, the object to be transferred into a living body by the living body transfer device is not limited to a cell population; it may also be a solid substance such as a pharmaceutical agent.

Claims

1. A biological transfer device for placing an object into a biological body, wherein: Equipped with multiple movable units, Each movable unit includes one or more needle-shaped portions extending along the first direction, each needle-shaped portion having a cylindrical shape capable of accommodating the object therein. Each movable unit is configured to be movable along the first direction. It also includes a control unit for controlling the movements of the plurality of movable units. The control unit is configured as follows: moving the movable unit toward the organism so that the needle-shaped portion enters the organism, The movable unit is stopped at a timing different from when the other movable units among the plurality of movable units stop moving.

2. The living body transfer device according to claim 1, wherein The other movable unit is adjacent to the movable unit that has moved toward the living body in order to cause the needle-shaped portion to enter the living body among the plurality of movable units.

3. The living body transfer device according to claim 1, wherein The control unit is configured as follows: The movable unit is moved in a direction away from the biological body to remove the needle-shaped portion from the biological body. The movable unit starts moving at a timing different from the timing at which the other movable units among the plurality of movable units start moving.

4. The living body transfer device according to any one of claims 1 to 3, wherein The control unit is configured as follows: The movable units are controlled so that the objects received by the needle-shaped portions are released from the needle-shaped portions after all the needle-shaped portions of the plurality of movable units have completed entry into the living body.

5. The living body transfer device according to any one of claims 1 to 3, wherein The control unit is configured as follows: Each movable unit is controlled so as to continuously perform the insertion of the needle-shaped portion into the living body, the release of the object received by the needle-shaped portion from the needle-shaped portion, and the extraction of the needle-shaped portion from the living body.

6. The living body transfer device according to any one of claims 1 to 3, wherein The plurality of movable units include at least five needle-shaped portions in total. In the above-mentioned biological transfer device, the needle-shaped portion is arranged at a ratio of 1 cm 2 For density configurations of 5 or more.

7. The living body transfer device according to any one of claims 1 to 3, wherein This living body transfer device is used to place the above-mentioned object, which is a cell aggregate containing skin stem cells, into the above-mentioned living body.

8. A biological transfer device for placing an object into a biological body, wherein: Equipped with multiple movable units, Each movable unit includes one or more needle-shaped portions extending along the first direction, each needle-shaped portion having a cylindrical shape capable of accommodating the object therein. Each movable unit is configured to be movable along the first direction. It also includes a control unit for controlling the movements of the plurality of movable units. The control unit is configured as follows: The movable unit is moved in a direction away from the biological body to remove the needle-shaped portion from the biological body. The movable unit starts moving at a timing different from the timing at which the other movable units among the plurality of movable units start moving.

9. The living body transfer device according to claim 8, wherein: The other movable unit is adjacent to the movable unit that moves in a direction away from the living body in order to extract the needle-shaped portion from the living body among the plurality of movable units.

10. The living body transfer device according to claim 9, wherein The control unit is configured as follows: moving the movable unit toward the organism so that the needle-shaped portion enters the organism, The movable unit is stopped at a timing different from when the other movable units among the plurality of movable units stop moving.

11. The living body transfer device according to claim 9 or 10, wherein: The control unit is configured as follows: The movable units are controlled so that the objects received by the needle-shaped portions are released from the needle-shaped portions after all the needle-shaped portions of the plurality of movable units have completed entry into the living body.

12. The living body transfer device according to claim 9 or 10, wherein: The control unit is configured as follows: Each movable unit is controlled so as to continuously perform the insertion of the needle-shaped portion into the living body, the release of the object received by the needle-shaped portion from the needle-shaped portion, and the extraction of the needle-shaped portion from the living body.

13. The living body transfer device according to claim 9 or 10, wherein: The plurality of movable units include at least five needle-shaped portions in total. In the above-mentioned biological transfer device, the needle-shaped portion is arranged at a ratio of 1 cm 2 For density configurations of 5 or more.

14. The living body transfer device according to claim 9 or 10, wherein: This living body transfer device is used to place the above-mentioned object, which is a cell aggregate containing skin stem cells, into the above-mentioned living body.

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