A detection module for in-situ Raman spectroscopy analysis of liquids

Abstract

The present invention relates to a detection module for in-situ Raman spectroscopy analysis of liquids, comprising: a hemispherical groove buckle sampling base, which is composed of a hemispherical groove, a groove channel port and a flat plate bracket, wherein the inner curved surface of the hemispherical groove is smooth, the center of the sphere is arranged in the section, and the edge of the section is extended to an octagon to form a drainage plane; the groove channel port is arranged above the hemispherical groove, and the section is the drainage plane; the flat plate bracket is arranged on both sides of the groove channel port, and buckles are arranged on the edge of the flat plate bracket for hanging on the cubic water tank slide rail; the cubic water tank comprises a water tank body, a water inlet and a water outlet, the water inlet and the water outlet are lower than the edge of the water tank body, and slide rails are arranged on the left and right pool walls of the water tank body, and at least three stepped gears are arranged on the slide rails; the hemispherical groove buckle sampling base is arranged above the cubic water tank. Compared with the prior art, the present invention has the advantages of high signal strength, convenience and high efficiency.

Description

Technical Field

[0001] The present invention relates to the field of Raman spectroscopy analysis, and in particular to a detection module for in-situ Raman spectroscopy analysis of liquid. Background Art

[0002] Raman spectroscopy analysis has attracted widespread attention for its ability to achieve simple, fast, non-destructive, separation-free, original, non-destructive, efficient and direct determination of molecular vibration peaks and qualitative analysis of each target, especially the detection of multi-component organic samples. However, the signals of in-situ Raman spectroscopy analysis are generally weak, and it takes a long time to collect and accumulate signal strength, that is, there is a certain delay. The flow of fluid samples is unstable, and it is difficult to complete the fixed-point collection of Raman signals, especially in the analysis of high-flow rate, low-concentration, and multi-component fluids. For this reason, staff are often required to set up additional sampling points, which can easily destroy the timeliness of in-situ samples and introduce new environmental pollution, and add cumbersome detection steps, which is not conducive to production line application. In this regard, people have proposed to improve the sensitivity of in-situ Raman analysis from aspects such as signal recovery methods and target sample enrichment, which is mainly achieved by designing a multifunctional sampling / detection substrate.

[0003] At present, there are various types of Raman spectroscopy detection substrates, including planar, grooved, 3D cone, SERS and other styles. Specially constructed substrates and magnetic materials, targeting groups, filter membranes and other functional enrichments and enhancements are used to enhance the target components in liquid samples, and the recovery efficiency of Raman signals is improved to achieve rapid and accurate analysis of low-concentration molecules to be tested. Such detection substrates are generally easy to replicate in batches, economical and effective. However, a universal cuvette detection pool is often used for the detection of liquid samples. It does not have the sampling and enrichment function, and the detection method is through-type analysis, without obvious enrichment function, and has low help for detection. The use of a flat / non-spherical substrate will result in a small amount of sample and uneven concentration in different laser irradiation areas, which is not conducive to the focused detection and accurate qualitative analysis of liquid samples. The use of SERS to enhance the substrate potential will use precious metal materials, which is likely to cause pollution and damage to the sample environment. In order to achieve enrichment-enhanced detection, there are also Raman spectroscopy detection substrates that are prepared with multifunctional special detection substrates through composite magnetic materials, screening and filtering materials, targeted recognition groups and other components. Such multifunctional substrates are usually complex to prepare and require modification of expensive materials such as nucleic acids, antibodies, and peptides. They have high consumption in batch production and are not suitable for high-throughput in-situ detection on the production line. Summary of the invention

[0004] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and to provide a detection module for in-situ Raman spectroscopy analysis of liquids which has high signal intensity, does not pollute samples, and is convenient and efficient.

[0005] The purpose of the present invention can be achieved by the following technical solutions:

[0006] A detection module for in-situ Raman spectroscopy analysis of liquids, comprising:

[0007] The hemispherical groove buckle sampling base is composed of a hemispherical groove, a groove channel opening and a flat plate bracket. The inner curved surface of the hemispherical groove is smooth, the center of the sphere is set in the section, and the edge of the section is extended front and back to form an octagon to form a drainage plane; the groove channel opening is set above the hemispherical groove, and the section is the drainage plane; the flat plate bracket is set on both sides of the groove channel opening, and buckles are set on the edge of the flat plate bracket for hanging on the cubic water tank slide rail;

[0008] A cubic water tank, comprising a water tank body, a water inlet and a water outlet, wherein the water inlet and the water outlet are lower than the edge of the water tank body, and slide rails are arranged on the left and right sides of the water tank body, and the slide rails are provided with at least three stepped gears;

[0009] The hemispherical groove buckle sampling base is arranged above the cubic water reservoir.

[0010] The three-stage stepped gear is set as:

[0011] When the hemispherical groove buckle sampling base is hung on the first gear of the cubic water tank slide rail through the buckle, the bottom edge of the hemispherical groove buckle sampling base is 5mm lower than the bottom of the water inlet / outlet;

[0012] When the hemispherical groove buckle sampling base is hung on the third gear of the cubic water tank slide rail through the buckle, the bottom edge of the hemispherical groove buckle sampling base is 5 mm lower than the bottom of the inlet / outlet and there is a gap between the hemispherical groove buckle sampling base and the water outlet.

[0013] The side length of the water reservoir body is not less than 10 cm and not more than 50 cm.

[0014] The diameter of the hemispherical groove is no more than 5 cm.

[0015] The diameter of the hemispherical groove is 2 cm.

[0016] When the sample flow rate is 0-1 cc / min, the side length of the cubic water reservoir is 10 cm, and the hemispherical groove buckle sampling base is hung on the first gear of the cubic water reservoir slide rail and close to the water inlet of the cubic water reservoir;

[0017] When the sample flow rate is 1-1000 cc / min, the side length of the cubic water reservoir is 10 cm, the hemispherical groove buckle sampling base is hung on the first-level gear of the cubic water reservoir slide rail, and slides on the first-level gear according to the flow rate to control the sample flow rate in the hemispherical groove.

[0018] The detection module also includes a multi-column caliper baffle component, the top shape of the multi-column caliper baffle component matches the shape of the groove channel opening, the bottom is evenly paved with cylindrical columns, and the interval between the centers of the columns is greater than the diameter of the columns.

[0019] The diameter of the cylindrical columns is 2 mm, and the interval between the centers of the columns is 3 mm.

[0020] When the sample flow rate is 1000-3000cc / min, the side length of the cubic water reservoir is not less than 10 cm and not more than 15 cm, the hemispherical groove buckle sampling base is hung on the second or higher gear of the cubic water reservoir slide rail, and the multi-column caliper flow blocking component is arranged above the hemispherical groove buckle sampling base;

[0021] When the sample flow rate is above 3000 cc / min, the side length of the cubic water reservoir is not less than 15 cm and not more than 20 cm, the hemispherical groove buckle sampling base is hung on the third gear of the cubic water reservoir slide rail, and the multi-column caliper flow blocking component is arranged above the hemispherical groove buckle sampling base.

[0022] The hemispherical groove buckle sampling base material is ordinary glass or quartz glass, and the cubic water reservoir and multi-column caliper flow-blocking component materials are glass, stainless steel or polytetrafluoroethylene.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] (1) The present invention utilizes a hemispherical groove design to hold liquid samples and enrich spectral signals, while effectively buffering the fluid flow rate. Compared with direct detection or planar base detection methods, the signal intensity is increased by 2-10 times.

[0025] (2) The present invention selects different gears for different flow rates, and can control the flow rate by using a multi-column caliper flow blocking component. It can greatly reduce the flow rate of the fluid sample in the hemispherical groove sampling pool and maintain stability, and realize delayed detection such as Raman spectroscopy analysis in an in-situ fixed-point area, meeting the needs of fluid analysis in various situations.

[0026] (3) For high-flow rate fluid sample detection, the hemispherical groove buckle sampling base and the cubic water reservoir are used for diversion, and the multi-column caliper flow blocking component is used for flow blocking and turbulence effects to prevent the fluid from scouring the sampling pool and effectively reduce the local flow velocity of the fluid, so that in-situ sampling can be achieved while performing fixed-point detection.

[0027] (3) The detection module of the present invention is immersed in the fluid, and the sample in the pool is continuously updated as the fluid flows, thereby completing the sampling without destroying the original state of the sample and causing no damage or contamination to the sample.

[0028] (4) The groove sampling pool of the present invention is made of quartz or glass, and has a smooth inner surface; the groove is in a regular hemispherical shape, and the center of the sphere is located in the horizontal section of the plane around the substrate, which effectively increases the Raman signal collection efficiency.

[0029] (5) The detection substrate of the present invention has a simple structure, is easy to replicate, has low mass production cost, and is suitable for high-throughput in-situ detection on the production line. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a schematic diagram of the structure of the detection module of the present invention, wherein 1-hemispherical groove buckle sampling base, 2-cubic water reservoir, 3-multi-column caliper flow blocking component;

[0031] Figure 2 This is a schematic diagram of the structure of a hemispherical groove buckle sampling base, wherein 4-slide rail buckle, 5-guide cavity, 6-guide plate, 7-sample pool, 8-support plate;

[0032] Figure 3 It is a schematic diagram of the cubic water storage tank structure of the present invention, wherein 9 is a fluid tank, 10 is a first-stage slide rail, 11 is a second-stage slide rail, 12 is a third-stage slide rail, and 13 is a conduit port;

[0033] Figure 4 It is a schematic diagram of the structure of the multi-column caliper flow-blocking component of the present invention, wherein 14 is a detection window, 15 is a toothed plate, 16 is a long flow-blocking column, and 17 is a short flow-blocking column;

[0034] Figure 5 This is a schematic diagram of the application of the present invention when the sample flow rate is 0-1 cc / min;

[0035] Figure 6 It is a schematic diagram of the application of the present invention when the sample flow rate is 1-1000 cc / min;

[0036] Figure 7 This is a schematic diagram of the application of the present invention when the sample flow rate is 1000-3000cc / min;

[0037] Figure 8 It is a schematic diagram of the application of the present invention when the sample flow rate is above 3000 cc / min;

[0038] Fig. 9 This is a comparison chart of the enhancement effects of hemispherical grooves with different radii;

[0039] Fig.10 This is the in-situ Raman spectroscopy detection enhancement test diagram when the radius of the hemispherical groove is 2 cm. DETAILED DESCRIPTION

[0040] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. This embodiment is implemented based on the technical solution of the present invention, and provides a detailed implementation method and specific operation process, but the protection scope of the present invention is not limited to the following embodiments.

[0041] A detection module for in-situ Raman spectroscopy analysis of liquids, such as Figure 1 As shown, including:

[0042] (1) Hemispherical groove snap sampling base

[0043] like Figure 2 As shown, the spherical groove buckle sampling base is composed of a hemispherical groove, a groove channel opening and a flat plate bracket. The inner curved surface of the hemispherical groove is smooth, the center of the sphere is located in the section, and the edge of the section is extended front and back to form an octagon to form a drainage plane; the groove channel opening is located above the hemispherical groove, and the section is a drainage plane for controlling the amount of fluid inflow; the flat plate bracket is located on both sides of the groove channel opening, and buckles are provided on the edge of the flat plate bracket for hanging on the cubic water tank slide rail.

[0044] The hemispherical groove has a diameter of no more than 5 cm and a diameter of 2 cm, which is most suitable for in-situ Raman spectroscopy detection. Fig. 9 As shown in the figure, the Raman detection enhancement test diagram of the groove substrate with a spherical radius of 2 cm is as follows Fig.10 shown.

[0045] The hemispherical groove buckle sampling base is made of ordinary glass or quartz glass, and the quartz glass material has a better effect.

[0046] (2) Cubic water reservoir

[0047] like Figure 3 As shown, the cubic water tank includes a water tank body, a water inlet and a water outlet, wherein the water inlet and the water outlet are lower than the edge of the water tank body, and slide rails are provided on the left and right side walls of the water tank body, and the slide rails are provided with at least three stepped gears.

[0048] The three-stage stepped gear is set as:

[0049] When the hemispherical groove buckle sampling base is hung on the first gear of the cubic water tank slide rail through the buckle, the bottom edge of the hemispherical groove buckle sampling base is 5mm lower than the bottom of the water inlet / outlet;

[0050] When the hemispherical groove buckle sampling base is hung on the third gear of the cubic water tank slide rail through the buckle, the bottom edge of the hemispherical groove buckle sampling base is 5 mm lower than the bottom of the inlet / outlet and there is a gap between the hemispherical groove buckle sampling base and the water outlet.

[0051] The side length of the water reservoir body is not less than 10 cm and not more than 50 cm.

[0052] The water reservoir body is made of non-corrosive materials such as glass, stainless steel, polytetrafluoroethylene, etc.

[0053] (3) Multi-column caliper flow-blocking component

[0054] like Figure 4 As shown, the top shape of the multi-column caliper flow-blocking component matches the shape of the groove channel opening, and the bottom is evenly paved with cylindrical columns, and the interval between the centers of the columns is greater than the diameter of the columns.

[0055] The diameter of the cylindrical columns is 2 mm, and the interval between the centers of the columns is 3 mm.

[0056] The multi-column caliper flow-blocking component is made of a material with sufficient density and not prone to corrosion, such as glass, stainless steel, polytetrafluoroethylene, etc.

[0057] According to different sample flow rates, different combinations of the above components can be used to effectively control the flow rate of the fluid in the groove sampling pool to be suitable for in-situ spectral analysis under different circumstances.

[0058] When the sample flow rate is 0~1 cc / min, Figure 5 As shown, the side length of the cubic water reservoir is 10 cm, and the hemispherical groove buckle sampling base is hung on the first gear of the cubic water reservoir slide rail and close to the water inlet of the cubic water reservoir;

[0059] When the sample flow rate is 1-1000 cc / min, Figure 6 As shown, the side length of the cubic water reservoir is 10 cm, the hemispherical groove buckle sampling base is hung on the first gear of the cubic water reservoir slide rail, and slides on the first gear according to the flow rate to control the sample flow rate in the hemispherical groove;

[0060] When the sample flow rate is 1000-3000cc / min, Figure 7 As shown, the side length of the cubic water reservoir is not less than 10 cm and not more than 15 cm, the hemispherical groove buckle sampling base is hung on the second or higher gear of the cubic water reservoir slide rail, and the multi-column caliper flow blocking component is arranged above the hemispherical groove buckle sampling base;

[0061] When the sample flow rate is above 3000 cc / min, Figure 8 As shown, the side length of the cubic water reservoir is not less than 15 cm and not more than 20 cm, the hemispherical groove buckle sampling base is hung on the third gear of the cubic water reservoir slide rail, and the multi-column caliper blocking component is arranged above the hemispherical groove buckle sampling base.

[0062] The hemispherical groove buckle sampling base and the cubic water reservoir are cleaned with strong acid and alcohol-base solution before use, which can improve the hydrophilicity of the base and achieve better flow blocking effect.

[0063] The preferred specific embodiments of the present invention are described in detail above. It should be understood that a person skilled in the art can make many modifications and changes based on the concept of the present invention without creative work. Therefore, any technical solution that can be obtained by a person skilled in the art through logical analysis, reasoning or limited experiments based on the concept of the present invention on the basis of the prior art should be within the scope of protection determined by the claims.

Claims

1. A detection module for in-situ Raman spectroscopy analysis of liquids, characterized in that: include: The hemispherical groove buckle sampling base is composed of a hemispherical groove, a groove channel opening and a flat plate bracket. The inner curved surface of the hemispherical groove is smooth, the center of the sphere is set in the section, and the edge of the section is extended front and back to form an octagon to form a drainage plane; the groove channel opening is set above the hemispherical groove, and the section is the drainage plane; the flat plate bracket is set on both sides of the groove channel opening, and buckles are set on the edge of the flat plate bracket for hanging on the cubic water tank slide rail; A cubic water tank, comprising a water tank body, a water inlet and a water outlet, wherein the water inlet and the water outlet are lower than the edge of the water tank body, and slide rails are arranged on the left and right sides of the water tank body, and the slide rails are provided with at least three stepped gears; The hemispherical groove buckle sampling base is arranged above the cubic water reservoir.

2. A detection module for in-situ Raman spectroscopy analysis of liquid according to claim 1, characterized in that: The three-step gear position is set as: When the hemispherical groove buckle sampling base is hung on the first gear of the cubic water tank slide rail through the buckle, the bottom edge of the hemispherical groove buckle sampling base is 5mm lower than the bottom of the water inlet / outlet; When the hemispherical groove buckle sampling base is hung on the third level of the cubic water tank slide rail through the buckle, there is a gap between the hemispherical groove buckle sampling base and the water outlet.

3. The detection module for in-situ Raman spectroscopy analysis of liquid according to claim 1, characterized in that: The side length of the water reservoir body is not less than 10 cm and not more than 50 cm.

4. The detection module for in-situ Raman spectroscopy analysis of liquid according to claim 1, characterized in that: The diameter of the hemispherical groove is no more than 5 cm.

5. The detection module for in-situ Raman spectroscopy analysis of liquid according to claim 1, characterized in that: The diameter of the hemispherical groove is 2 cm.

6. A detection module for in-situ Raman spectroscopy analysis of liquid according to claim 1 or 3, characterized in that: When the sample flow rate is 0-1 cc / min, the side length of the cubic water reservoir is 10 cm, and the hemispherical groove buckle sampling base is hung on the first gear of the cubic water reservoir slide rail and close to the water inlet of the cubic water reservoir; When the sample flow rate is 1-1000 cc / min, the side length of the cubic water reservoir is 10 cm, the hemispherical groove buckle sampling base is hung on the first-level gear of the cubic water reservoir slide rail, and slides on the first-level gear according to the flow rate to control the sample flow rate in the hemispherical groove.

7. The detection module for in-situ Raman spectroscopy analysis of liquid according to claim 1, characterized in that: The detection module also includes a multi-column caliper baffle component, the top shape of the multi-column caliper baffle component matches the shape of the groove channel opening, the bottom is evenly paved with cylindrical columns, and the interval between the centers of the columns is greater than the diameter of the columns.

8. The detection module for in-situ Raman spectroscopy analysis of liquid according to claim 7, characterized in that: The diameter of the cylindrical columns is 2 mm, and the interval between the centers of the columns is 3 mm.

9. A detection module for in-situ Raman spectroscopy analysis of liquid according to claim 1 or 7, characterized in that: When the sample flow rate is 1000-3000cc / min, the side length of the cubic water reservoir is not less than 10 cm and not more than 15 cm, the hemispherical groove buckle sampling base is hung on the second or higher gear of the cubic water reservoir slide rail, and the multi-column caliper flow blocking component is arranged above the hemispherical groove buckle sampling base; When the sample flow rate is above 3000 cc / min, the side length of the cubic water reservoir is not less than 15 cm and not more than 20 cm, the hemispherical groove buckle sampling base is hung on the third gear of the cubic water reservoir slide rail, and the multi-column caliper flow blocking component is arranged above the hemispherical groove buckle sampling base.

10. The detection module for in-situ Raman spectroscopy analysis of liquid according to claim 1, characterized in that: The hemispherical groove buckle sampling base material is ordinary glass or quartz glass, and the cubic water reservoir and multi-column caliper flow-blocking component materials are glass, stainless steel or polytetrafluoroethylene.

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