A sample concentration device for capillary electrophoresis

Abstract

This application relates to a sample concentration device for capillary electrophoresis, belonging to the field of capillary electrophoresis equipment, including a first housing and a second housing. The first housing is movably connected to the second housing. A telescopic rod is fixedly installed near the middle position of the inner cavity of the second housing. A connecting plate is fixedly installed on the outer surface of the upper end of the telescopic rod. Two groups of test tubes are symmetrically arranged on the inner bottom surface of the second housing. A capillary is penetrated and connected near the middle position of the outer surface of the upper end of the piston; by setting the first auxiliary mechanism, the present invention can avoid the problems in the prior art that when cleaning the capillary after using the capillary electrophoresis equipment, most of them first disassemble the capillary and then clean it manually. Therefore, when cleaning the capillary, the labor cost is high and the operation is troublesome.

Description

Technical Field

[0001] This application relates to the field of capillary electrophoresis equipment, and in particular to a sample concentration device for capillary electrophoresis. Background Art

[0002] Capillary electrophoresis (CE), also known as high-performance capillary electrophoresis (HPCE), is a new type of liquid-phase separation technology with a capillary as the separation channel and a high-voltage direct current electric field as the driving force. Capillary electrophoresis actually includes electrophoresis, chromatography, and their intersections, enabling analytical chemistry to move from the microliter level to the nanoliter level and making single-cell analysis, and even single-molecule analysis possible. The separation and analysis of biological macromolecules such as proteins, which have long troubled us, have thus taken a new turn.

[0003] In the prior art, after the capillary electrophoresis equipment is used, when cleaning the capillary, most of them first disassemble the capillary and then clean it manually. Therefore, when cleaning the capillary, the labor cost is high and the operation is troublesome. At the same time, in the existing capillary electrophoresis equipment, although there are heat dissipation holes on both the left and right sides, during daily use, dust is likely to accumulate inside the electrophoresis instrument, thereby reducing its heat dissipation efficiency and affecting the detection results of the electrophoresis equipment, which is not conducive to the normal use of the electrophoresis equipment. Summary of the Invention

[0004] In order to solve the problems raised in the above background art, this application provides a sample concentration device for capillary electrophoresis.

[0005] The sample concentration device for capillary electrophoresis provided by this application adopts the following technical solutions:

[0006] A sample concentration device for capillary electrophoresis includes a first housing and a second housing. The first housing is movably connected to the second housing. An expansion rod is fixedly installed near the middle position of the inner cavity of the second housing. A connecting plate is fixedly installed on the outer surface of the upper end of the expansion rod. Two groups of test tubes are symmetrically arranged on the inner bottom surface of the second housing. Two groups of pistons are symmetrically and fixedly installed on the inner top surface of the first housing. A capillary is connected through the outer surface of the upper end of the piston near the middle position. Electrodes are connected to the outer surfaces of the upper ends of the two groups of pistons near one side of the capillary. A first auxiliary mechanism is arranged on the outer surface of the upper end of the first housing near one side edge position.

[0007] Preferably, the first auxiliary mechanism includes a third housing fixedly installed on the outer surface of the upper end of the first housing near one side edge. A first connecting pipe penetrates and connects through the outer surface of one side of the third housing near the lower end. Inside the first housing near the upper end, a second connecting pipe is provided. The first connecting pipe and the second connecting pipe penetrate and connect. Inside the first housing, a connecting groove is opened on the outer side of the capillary tube. The inner bottom surface of the second connecting pipe penetrates and connects with evenly distributed spray nozzles. On the inner bottom surface of the connecting groove near the lower end of the capillary tube, an electromagnet is fixedly installed. On the inner bottom surface of the connecting groove near one side of the electromagnet, a drainage groove is penetrated and opened. Near the middle position on the outer surface of one side of the electromagnet, an elastic column is fixedly installed. One end of the elastic column is fixedly installed with a first magnet. The outer surface of the capillary tube is movably connected with a collar. One end of the collar is fixedly connected with the first magnet. On the inner surface of the collar, evenly distributed brush hairs are fixedly installed.

[0008] Preferably, a heating wire is fixedly installed on the bottom wall of the third housing near the middle position.

[0009] Preferably, filter nets are fixedly installed on both inner surfaces of the connecting groove near the edge positions. Fans are fixedly installed on the inner surface of the connecting groove near one side of the spray nozzles. A second auxiliary mechanism is provided on the inner surface of the connecting groove near one side of the fans.

[0010] Preferably, a box body is fixedly installed on the inner surface of the connecting groove near the middle position between the fans and the filter nets. Uniformly distributed connecting blocks are opened on both outer surfaces of the box body. A metal block is fixedly installed on the inner surface of the box body near the middle position.

[0011] Preferably, a feeding port penetrates and connects through the inner bottom surface of the box body near the middle position. Inside the first housing, a storage box is fixedly connected near the lower end of the feeding port. The lower end of the feeding port penetrates and connects with the storage box.

[0012] Preferably, a second magnet is fixedly installed on the outer surface of one side of the storage box. A third magnet is fixedly installed on the inner surface of the first housing near the second magnet.

[0013] Preferably, the second auxiliary mechanism includes a third connecting pipe fixedly installed on the inner surface of the connecting groove near the lower end of the second connecting pipe. An air bag penetrates and connects through the outer surface of the upper end of the connecting groove. The air bag penetrates and connects with the second connecting pipe.

[0014] In summary, the present application includes the following beneficial technical effects:

[0015] (1) In the present invention, by providing the first auxiliary mechanism, it is possible to avoid the problems in the prior art that when cleaning the capillary after using a capillary electrophoresis device, most of them first disassemble the capillary and then clean it manually. As a result, when cleaning the capillary, the labor cost is high and the operation is troublesome.

[0016] (2) When the capillary is in use in the present invention, the fan is turned on through the control system to generate an air flow. During the flow of the air flow, the outer surface of the capillary can be cooled, improving the working effect of the capillary. By providing a filter screen, it is possible to avoid the problem that large foreign matters in the outside world enter the inside of the connection groove and affect the heat dissipation of the capillary. By providing a metal block, the metal block is electrified through the control system to make the metal block positively charged, forming a stable electric field between the metal block and the first housing. When the gas passes through the connection groove, the electric field ionizes and charges the dust, causing the dust to move towards the metal block, thereby adsorbing and cleaning the small particle dust in the gas. When the power supply is stopped, the adsorption force of the metal block on the dust disappears, and the dust falls into the storage box through the discharge port under the action of gravity and is stored, thus avoiding the problem that too much dust adsorbed on the metal block affects the working effect of the metal block. When it is necessary to clean the dust in the storage box, the storage box can be taken out by overcoming the magnetic force between the second magnet and the third magnet manually, which is convenient and fast, thus avoiding the problem that in the existing capillary electrophoresis device, although there are heat dissipation holes on both the left and right sides, during daily use, dust is likely to accumulate inside the electrophoresis instrument, thereby reducing its heat dissipation efficiency and affecting the detection result of the electrophoresis device, which is not conducive to the normal use of the electrophoresis device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is the overall structural schematic diagram of a sample concentration device for capillary electrophoresis in an embodiment of the present application;

[0018] Figure 2 is the complete structural schematic diagram of the connection between the first housing and the second housing of a sample concentration device for capillary electrophoresis in an embodiment of the present application;

[0019] Figure 3 is the overall sectional structural schematic diagram of a sample concentration device for capillary electrophoresis in an embodiment of the present application;

[0020] Figure 4 is a sample concentration device for capillary electrophoresis in an embodiment of the present application Figure 2 the enlarged structural schematic diagram at A in;

[0021] Figure 5 is a sample concentration device for capillary electrophoresis in an embodiment of the present application Figure 2 the enlarged structural schematic diagram at B in;

[0022] Figure 6 It is an enlarged view of the second auxiliary mechanism in a sample concentration device for capillary electrophoresis in an embodiment of the present application.

[0023] Description of reference numerals: 1. First housing; 2. Second housing; 3. Telescopic rod; 4. Connecting plate; 5. Test tube; 6. Piston; 7. Capillary; 8. Electrode; 9. First auxiliary mechanism; 91. Third housing; 92. First connecting pipe; 93. Second connecting pipe; 94. Sprayer; 95. Connecting groove; 96. Drainage groove; 97. Electromagnet; 98. Elastic column; 99. First magnet; 910. Collar; 911. Brush; 912. Resistance wire; 913. Fan; 914. Filter screen; 915. Box body; 916. Connecting block; 917. Metal block; 918. Second auxiliary mechanism; 9181. Third connecting pipe; 9182. Airbag; 919. Feeding port; 9110. Storage box; 9111. Second magnet; 9112. Third magnet. Detailed implementation manners

[0024] The following will Figures 1-6 make a further detailed description of the present application.

[0025] Embodiment 1

[0026] An embodiment of the present application discloses a sample concentration device for capillary electrophoresis. Refer to Figures 1-6 , a sample concentration device for capillary electrophoresis, includes a first housing 1 and a second housing 2. The first housing 1 and the second housing 2 are movably connected. A telescopic rod 3 is fixedly installed near the middle position in the inner cavity of the second housing 2. A connecting plate 4 is fixedly installed on the outer surface of the upper end of the telescopic rod 3. Two groups of test tubes 5 are symmetrically arranged on the inner bottom surface of the second housing 2. Two groups of pistons 6 are symmetrically and fixedly installed on the inner top surface of the first housing 1. A capillary 7 penetrates through the outer surface of the upper end of the piston 6 near the middle position. Electrodes 8 are connected to the outer surfaces of the upper ends of the two pistons 6 on one side near the capillary 7. A first auxiliary mechanism 9 is arranged on the outer surface of the upper end of the first housing 1 near one side edge position.

[0027] Refer to Figures 2-4, the first auxiliary mechanism 9 includes a third housing 91 fixedly installed on the outer surface of the upper end of the first housing 1 near one side edge position. A first connecting pipe 92 penetrates and connects to the outer surface of one side of the third housing 91 near the lower end position. A second connecting pipe 93 is arranged inside the first housing 1 near the upper end position. The first connecting pipe 92 and the second connecting pipe 93 penetrate and connect. A connecting groove 95 is opened outside the capillary 7 inside the first housing 1. The inner bottom surface of the second connecting pipe 93 penetrates and connects with uniformly distributed spray nozzles 94. An electromagnet 97 is fixedly installed on the inner bottom surface of the connecting groove 95 near the lower end position of the capillary 7. A drain groove 96 penetrates through the inner bottom surface of the connecting groove 95 near one side position of the electromagnet 97. An elastic column 98 is fixedly installed on the outer surface of one side of the electromagnet 97 near the middle position. One end of the elastic column 98 is fixedly installed with a first magnet 99. A collar 910 is movably connected to the outer surface of the capillary 7. One end of the collar 910 is fixedly connected to the first magnet 99. Uniformly distributed bristles 911 are fixedly installed on the inner surface of the collar 910.

[0028] A heating wire 912 is fixedly installed on the bottom wall of the third housing 91 near the middle position;

[0029] During operation, first pull out the second housing 2 from the first housing 1, then place the test tube filled with buffer solution on the connecting plate 4, and then push the second housing 2 into the first housing 1. Then, control the telescopic rod 3 to move upward through the control system until the test tube 5 is inserted into the piston 6. Then, energize the electrode 8 through the control system to start the concentration work. When the concentration is completed, pull out the second housing 2 from the first housing 1, take out the test tube 5 filled with the solution, put it into the test tube 5 filled with cleaning solution, and repeat the above work. Under the action of the electrode 8, the cleaning solution enters the interior of the capillary 7 to clean the capillary 7. Then, energize the resistance wire 912 through the control system. After the resistance wire 912 is energized, it heats the cleaning solution inside the third housing 91. Then, turn on the water pump. At this time, the heated cleaning solution flushes the capillary 7 through the first connecting pipe 92, the second connecting pipe 93, and the nozzle 94. The heated cleaning solution can effectively remove the grease attached to the inner wall of the capillary 7. Since the magnetic properties of the contact surface between the electromagnet 97 and the first magnet 99 are the same, after energizing the electromagnet 97 through the control system, a repulsive force is generated between the electromagnet 97 and the first magnet 99. The repulsive force causes the first magnet 99 to overcome the elastic force of the elastic column 98 and drive the collar 910 to move leftward on the outer surface of the capillary 7. When it moves to the upper edge position of the capillary 7, the control system stops energizing the electromagnet 97, and the repulsive force between the electromagnet 97 and the first magnet 99 disappears. The elastic force of the elastic column 98 recovering from the elastic deformation drives the first magnet 99 back to its original position. This cycle repeats, and the collar 910 drives the collar 910 to reciprocate on the outer surface of the capillary 7 to clean the outer surface of the capillary 7. The cleaned cleaning solution is discharged through the drain groove 96, thus avoiding the problem in the prior art that when cleaning the capillary after using a capillary electrophoresis device, most of them first disassemble the capillary and then clean it manually. Therefore, when cleaning the capillary, the labor cost is high and the operation is troublesome.

[0030] Embodiment 2

[0031] This application embodiment discloses a sample concentration device for capillary electrophoresis. Refer to Figures 1-6 , for a sample concentration device for capillary electrophoresis, filter nets 914 are fixedly installed on both inner surfaces of the connection groove 95 near the edge positions, and fans 913 are fixedly installed on both inner surfaces of the connection groove 95 near one side of the nozzle 94. A second auxiliary mechanism 918 is arranged on the inner surface of the connection groove 95 near one side of the fan 913.

[0032] Refer to Figures 5-6 , a box body 915 is fixedly installed on the inner surface of the connection groove 95 at the middle position between the fan 913 and the filter net 914. Uniformly distributed connection blocks 916 are formed on both outer surfaces of the box body 915. A metal block 917 is fixedly installed on the inner surface of the box body 915 near the middle position.

[0033] A blanking port 919 is connected through the inner bottom surface of the box body 915 near the middle position. A storage box 9110 is fixedly connected to the inside of the first housing 1 near the lower end of the blanking port 919. The lower end of the blanking port 919 is connected to the storage box 9110 through.

[0034] A second magnet 9111 is fixedly installed on the outer surface of one side of the storage box 9110. A third magnet 9112 is fixedly installed on the inner surface of the first housing 1 near the second magnet 9111.

[0035] The second auxiliary mechanism 918 includes a third connecting pipe 9181 fixedly installed on the inner surface of the connecting groove 95 near the lower end of the second connecting pipe 93. An airbag 9182 is connected through the upper outer surface of the connecting groove 95. The airbag 9182 is connected through the second connecting pipe 93.

[0036] Specifically, when the capillary 7 is in use, the fan 913 is turned on through the control system to generate an air flow. During the process of the air flow flowing, the outer surface of the capillary 7 can be dissipated heat, improving the working effect of the capillary 7. By setting the filter screen 914, it is possible to prevent large foreign impurities from entering the inside of the connecting groove 95 and affecting the heat dissipation of the capillary 7. By setting the metal block 917, the control system is used to energize the metal block 917 to make the metal block 917 positively charged, forming a stable electric field between the metal block 917 and the first housing 1. When the gas passes through the connecting groove 95, the electric field ionizes and charges the dust, causing the dust to move towards the metal block 917, thereby adsorbing and cleaning the small particle dust in the gas. When the power is stopped, the adsorption force of the metal block 917 on the dust disappears, and the dust falls into the storage box 9110 through the blanking port 919 for storage, thus avoiding the problem that too much dust adsorbed on the metal block 917 affects the working effect of the metal block 917. When it is necessary to clean the dust in the storage box 9110, by manually overcoming the magnetic force between the second magnet 9111 and the third magnet 9112, the storage box 9110 can be taken out conveniently and quickly, thus avoiding the problem that in the existing capillary electrophoresis equipment, although there are heat dissipation holes on both the left and right sides, during daily use, dust is likely to accumulate inside the electrophoresis instrument, thereby reducing its heat dissipation efficiency and affecting the detection results of the electrophoresis equipment, which is not conducive to the normal use of the electrophoresis equipment.

[0037] The above are all the preferred embodiments of this application, and the protection scope of this application is not limited by this. Therefore, all equivalent changes made according to the structure, shape, and principle of this application should be covered within the protection scope of this application.

Claims

1. A sample concentration device for capillary electrophoresis, comprising a first housing (1) and a second housing (2). Characterized in that, The first housing (1) is movably connected to the second housing (2). A telescopic rod (3) is fixedly installed near the middle position in the inner cavity of the second housing (2). A connecting plate (4) is fixedly installed on the outer surface of the upper end of the telescopic rod (3). Two groups of test tubes (5) are symmetrically arranged on the inner bottom surface of the second housing (2). Two groups of pistons (6) are symmetrically and fixedly installed on the inner top surface of the first housing (1). A capillary (7) is connected through the outer surface of the upper end of the piston (6) near the middle position. Electrodes (8) are connected to the outer surfaces of the upper ends of the two pistons (6) on one side near the capillary (7). A first auxiliary mechanism (9) is arranged on the outer surface of the upper end of the first housing (1) near one side edge position. The first auxiliary mechanism (9) includes a third housing (91) fixedly installed on the outer surface of the upper end of the first housing (1) near one side edge position. A first connecting pipe (92) is connected through the outer surface of one side of the third housing (91) near the lower end position. A second connecting pipe (93) is arranged inside the first housing (1) near the upper end position. The first connecting pipe (92) and the second connecting pipe (93) are connected through. A connecting groove (95) is opened outside the capillary (7) inside the first housing (1). Spray heads (94) evenly distributed are connected through the inner bottom surface of the second connecting pipe (93). An electromagnet (97) is fixedly installed on the inner bottom surface of the connecting groove (95) near the lower end position of the capillary (7). A drain groove (96) is opened through the inner bottom surface of the connecting groove (95) on one side near the electromagnet (97). An elastic column (98) is fixedly installed on the outer surface of one side of the electromagnet (97) near the middle position. A first magnet (99) is fixedly installed at one end of the elastic column (98). A collar (910) is movably connected to the outer surface of the capillary (7). One end of the collar (910) is fixedly connected to the first magnet (99). Uniformly distributed bristles (911) are fixedly installed on the inner surface of the collar (910).

2. The sample concentration device for capillary electrophoresis according to claim 1, Characterized in that, A heating wire (912) is fixedly installed on the bottom wall of the third housing (91) near the middle position.

3. The sample concentration device for capillary electrophoresis according to claim 1, Characterized in that, Filter meshes (914) are fixedly installed on the inner surfaces of both sides of the connecting groove (95) near the edge positions. Fans (913) are fixedly installed on the inner surfaces of the connecting groove (95) on one side near the spray heads (94). A second auxiliary mechanism (918) is arranged on the inner surface of the connecting groove (95) on one side near the fans (913).

4. The sample concentration device for capillary electrophoresis according to claim 3, Characterized in that, The inner surface of the connecting groove (95) is fixedly installed with a box body (915) near the middle position between the fan (913) and the filter net (914). The outer surfaces on both sides of the box body (915) are provided with evenly distributed connecting blocks (916), and a metal block (917) is fixedly installed on the inner surface of the box body (915) near the middle position.

5. A sample concentration device for capillary electrophoresis according to claim 4, characterized in that a blanking port (919) is connected through the inner bottom surface of the box body (915) near the middle position. A storage box (9110) is fixedly connected to the inside of the first housing (1) near the lower end of the blanking port (919), and the lower end of the blanking port (919) is connected through the storage box (9110).

6. A sample concentration device for capillary electrophoresis according to claim 5, characterized in that a second magnet (9111) is fixedly installed on the outer surface of one side of the storage box (9110), and a third magnet (9112) is fixedly installed on the inner surface of the first housing (1) near the side of the second magnet (9111).

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