A biological organic fertilizer for preventing and controlling underground pests, preparation method and application thereof

Abstract

The invention discloses a biological organic fertilizer for preventing and controlling underground pests, a preparation method and an application thereof, relates to the field of biological organic fertilizers, and solves the problem of pesticide residues and environmental pollution in the prior art when chemical pesticides are used to prevent and control pests. The method comprises the following steps: preparing Bacillus subtilis WSWFJ44 powder, preparing Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 spore powder; preparing oil-blight type organic fertilizer; compounding Bacillus subtilis WSWFJ44 powder, Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 spore powder with oil-blight type organic fertilizer to prepare biological organic fertilizer for preventing and controlling underground pests; the amount of effective live bacteria in Bacillus subtilis WSWFJ44 powder is not less than 100 billion / gram, and the amount of effective live bacteria of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 in the spore powder of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 is not less than 10 billion / gram. The biological organic fertilizer of the present invention can not only prevent and control underground pests, but also increase the population of beneficial microorganisms in the soil, and can also greatly reduce the application amount of chemical pesticides, reduce environmental pollution, and improve crop safety.

Description

Technical Field

[0001] The present invention relates to the technical field of bio-organic fertilizers, and in particular to a bio-organic fertilizer for preventing and controlling underground pests, a preparation method and an application thereof. Background Art

[0002] White grubs are a general term for the larvae of the superfamily Scarabaeoidea of ​​the order Coleoptera. They are also recognized underground pests in the world and can harm a variety of plants. They are the most harmful underground pests in recent years, causing huge losses to agricultural production. White grubs live in the soil all year round and mainly harm field crops such as wheat, corn, potatoes, beans, and peanuts. At the same time, the harm to tobacco should not be underestimated. The harm of white grubs to various crops is mainly manifested in eating germinating seeds and tender roots, biting off the rhizomes of wheat seedlings, and directly biting the tender fruits of peanuts or the tubers and roots of potatoes, sweet potatoes, and beets. Not only does it cause crop yield reduction, but the wounds are also easily infected by pathogens. According to survey statistics, 86% of the damage to the underground parts of plants is caused by white grubs.

[0003] Grubs are an important underground pest that harms tobacco. They are serious in Yunnan tobacco-growing areas and have a serious impact on Yunnan's tobacco industry. Cutworms, also known as earthworms and root cutters, go through the stages of eggs, larvae, pupae, and adults. The number of generations that occur each year varies with the climate in different places. They are very harmful to agricultural and forest seedlings, causing seedling shortages and broken ridges at the least, and destroying and reseeding at the worst. Tobacco planting is greatly affected.

[0004] In the 1950s and 1960s, organochlorine insecticides and organophosphorus insecticides were mainly used for underground pest control. However, these two agents are highly toxic, have high residues, are unsafe for humans and animals, and are now banned. In recent years, more and more studies have been conducted on chemical control of grubs. The chemical agents used mainly include phoxim, isofenphos-methyl, carbosulfan, imidacloprid, chlorpyrifos, trichlorfon, and biological pesticides such as azadirachtin and pyrethrin. In order to cooperate with the control of larvae, the control of adult scarab beetles is equally important. The main control agents used in the adult occurrence season are oxydemeton-methyl, trichlorfon, chlorpyrifos, and high-efficiency cypermethrin. At present, chemical control is still a widely used control method, especially when grubs occur in large numbers and other control methods cannot work immediately. Chemical control can reduce the population density of grubs to below the economically acceptable level in a short period of time. However, due to the damage caused by white grubs underground and the isolating effect of the soil, white grubs have high requirements on the method, dosage, period of use and residue of pesticides.

[0005] A large number of studies have shown that with the long-term use of chemical pesticides, pesticide residues and environmental pollution problems have become increasingly serious. In addition, the long-term use of chemical pesticides will lead to a decrease in the population density of microbial communities suitable for tobacco growth in the soil, affecting the balance of soil microbial communities, and causing the growth and development of tobacco plants to be affected to varying degrees. With the gradual ban on the use of highly toxic chemical pesticides, there are fewer and fewer effective agents for controlling white grubs, and the dosage form is single, and the drug resistance of white grubs is gradually increasing. Therefore, there is an urgent need to develop new control agents.

[0006] Biological control and ecological control have received widespread attention in recent years. Biological control mainly uses insecticide microbial agents to infect underground pests, allowing them to quickly parasitize on the pests and finally kill the pests. Considering a series of problems such as environmental factors and pesticide residues, the most ideal method for controlling grubs is biological control. Therefore, it is urgent to research and develop new microbial agents for controlling underground pests. Summary of the invention

[0007] The purpose of the present invention is to provide a biological organic fertilizer for controlling underground pests, a preparation method and an application thereof, which solves the technical problem of using chemical pesticides to control pests in the prior art, resulting in pesticide residues and environmental pollution. The various technical effects that can be produced by the preferred technical scheme of the present invention are described in detail below.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] The method for preparing the biological organic fertilizer for preventing and controlling underground pests of the present invention comprises the following steps:

[0010] preparing Bacillus subtilis WSWFJ44 bacterial powder, and preparing spore powder of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48;

[0011] Preparation of oil-based organic fertilizer by fermentation;

[0012] The prepared Bacillus subtilis WSWFJ44 spore powder, Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 spore powder are compounded with oil-blight type organic fertilizer to prepare biological organic fertilizer for preventing and controlling underground pests;

[0013] Among them, the amount of effective live bacteria in the Bacillus subtilis WSWFJ44 powder is not less than 100 billion / gram, and the amount of effective live bacteria of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 in the spore powder of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 is not less than 10 billion / gram.

[0014] The Bacillus subtilis of the present invention is numbered WSWFJ44, the deposit registration number is CGMCC No.20144, the deposit date is June 28, 2020, the deposit unit is the General Microbiology Center of China Microbiological Culture Collection Administration, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0015] The Beauveria sp. of the present invention is numbered WSWFJ49, the deposit registration number is CGMCC No. 20213, the deposit date is June 28, 2020, the deposit unit is the General Microbiology Center of China National Microbiological Culture Collection Administration, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0016] The Metarhizium sp. of the present invention is numbered WSWFJ48, the preservation registration number is CGMCC No.19926, the preservation date is June 28, 2020, the preservation unit is the General Microbiology Center of China Microbiological Culture Collection Administration, and the preservation address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0017] According to a preferred embodiment, during the compounding process, the weight ratio of Bacillus subtilis WSWFJ44 powder, Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 spore powder is 2:0.5:0.5.

[0018] According to a preferred embodiment, the preparation process of Bacillus subtilis WSWFJ44 powder comprises the following steps:

[0019] Activation of strains: Activate the Bacillus subtilis WSWFJ44 strain stored at -80°C on LB solid medium for 12h to 16h to obtain an activated strain;

[0020] Preparation of seed solution: Use a sterile inoculation loop to scrape a loop of activated strain and inoculate it into 50 ml LB liquid culture medium. Cultivate at a temperature of 36.5°C-37.5°C and a rotation speed of 160 rpm / min for 24 hours to obtain seed solution.

[0021] Preparation of fermentation liquid: The obtained seed liquid was inoculated into the workshop fermentation medium according to the ratio of the strain to the culture liquid volume of 1:120, and cultured at a temperature of 36.5°C-37.5°C and a rotation speed of 120 rpm / min for 24h-36h to obtain the fermentation liquid, which is the Bacillus subtilis WSWFJ44 bacterial suspension, and the effective viable bacterial count of the Bacillus subtilis WSWFJ44 bacterial suspension is 20.0×10 8 cfu / g or more;

[0022] The obtained fermentation liquid is centrifuged and then an adsorption protective agent is added, and then the fermentation liquid with the adsorption protective agent added is transferred to a powder spraying device to obtain Bacillus subtilis WSWFJ44 bacterial powder, and the amount of effective live bacteria in the Bacillus subtilis WSWFJ44 bacterial powder is not less than 100 billion / gram.

[0023] According to a preferred embodiment, during the activation of the strain, the LB solid culture medium includes the following components in concentrations: 10 g / L bacteriological peptone, 5 g / L yeast extract powder, 10 g / L sodium chloride, and 24 g / L agar powder; during the preparation of the seed solution, the LB liquid culture medium includes the following components in concentrations: 10 g / L bacteriological peptone, 5 g / L yeast extract powder, and 10 g / L sodium chloride.

[0024] According to a preferred embodiment, during the preparation of the fermentation broth, the fermentation medium includes the following ingredients in mass percentage: 1.0%-1.6% soybean powder, 0.1%-0.5% sucrose, 0.2%-0.6% peptone, 0.2%-0.6% corn flour, 0.3%-0.9% calcium carbonate, 0.1%-0.3% fish meal, 0.01%-0.08% ammonium sulfate, 0.02%-0.05% magnesium sulfate, 0.01%-0.04% potassium dihydrogen phosphate, 0.02%-0.05% dipotassium hydrogen phosphate, 0.01%-0.04% manganese sulfate, 0.01%-0.04% sodium hydroxide, 0.02%-0.03% sodium chloride, 0.5%-1.5% defoaming agent, and the balance is water, and the pH is adjusted to 6.8-7.5 with hydrochloric acid and sodium hydroxide.

[0025] According to a preferred embodiment, the preparation process of spore powder of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 comprises the following steps:

[0026] Preparation of seed solutions of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48: Activate the preserved Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 on PDA solid culture medium, inoculate into 200 mL PDA liquid culture medium after activation, place on a constant temperature shaker at 28°C and 180 r / min for culturing for 72 h, then inoculate the cultured bacterial suspension into 1000 mL PDA liquid culture medium at 1% inoculation amount, place on a constant temperature shaker at 28°C and 180 r / min for propagation for 72 h, to obtain seed solutions of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48;

[0027] Preparation of fermentation broth of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48: Add 600L of special fermentation medium to a fermentation tank with a volume of 1000L, add seed liquid of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 to the fermentation tank, and simmer at a ventilation volume of 35m 3 / h, temperature 28 ° C and rotation speed 160r / min, and fermented for 48h-72h to obtain the fermentation liquid of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48;

[0028] Solid fermentation: add the fermentation liquid of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 to the solid culture medium at a weight ratio of 1:10, add water to adjust the water content of the fermentation material to 60%-80%, mix evenly in a blender, spread the fermentation material in the fermentation tank after mixing, the height of the mixed material is 10cm-15cm, cover the top of the fermentation tank with a black shade net, and keep the temperature at 28℃-30℃ and the ventilation volume at 5m 3 / h conditions for 60 h, and after 60 h, open the black shade net at 30-35 ° C and a ventilation volume of 5m 3 / h conditions for fermentation for 36 hours, after 36 hours, add the mixed material into a drying barrel, and dry it at a temperature of 50°C-55°C until the water content of the material drops to 10%-15%, and then grind it into powder to obtain spore powder of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48, and the amount of effective live bacteria in the spore powder is not less than 10 billion / g.

[0029] According to a preferred embodiment, during the preparation of the seed solution of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48, the PDA liquid culture medium includes the following components in concentrations: 200 g / L potato and 20 g / L glucose; the PDA solid culture medium includes the following components in concentrations: 200 g / L potato, 20 g / L glucose, and 24 g / L agar.

[0030] According to a preferred embodiment, during the preparation of the fermentation broth of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48, the special fermentation medium includes the following components in concentrations: yeast extract 3g / L-5g / L, peptone 3g / L-5g / L, corn steep liquor powder 8g / L-10g / L, corn starch 3g / L-5g / L, dipotassium hydrogen phosphate 0.2g / L-0.5g / L, potassium dihydrogen phosphate 0.1g / L-0.3g / L, glucose 10g / L-15g / L and molasses powder 1g / L-10g / L, and the pH is adjusted to 6.5-7.0;

[0031] During the solid fermentation process, the solid culture medium includes the following components in parts by weight: 50 L-100 parts of corn flour, 300 L-400 parts of oil cake, 300 L-400 parts of peat, 100 L-200 parts of wheat bran, 100 L-200 parts of rice husk, and 50 L-100 parts of soybean powder, and water is added to adjust the moisture content to 60%-80%.

[0032] The biological organic fertilizer for preventing and controlling underground pests of the present invention is prepared by adopting the preparation method of any one of the technical solutions of the present invention.

[0033] The application of the bio-organic fertilizer for preventing and controlling underground pests according to any technical solution of the present invention is that the bio-organic fertilizer is applied as base fertilizer during the plant planting process.

[0034] The biological organic fertilizer for preventing and controlling underground pests, the preparation method and the application provided by the present invention have at least the following beneficial technical effects:

[0035] The biological organic fertilizer for preventing and controlling underground pests of the present invention is prepared by compounding spore powder of Bacillus subtilis WSWFJ44, spore powder of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 with oil-blight type organic fertilizer. On the one hand, the biological organic fertilizer for preventing and controlling underground pests of the present invention has biological control strains, which can effectively infect pests, thereby effectively preventing and controlling underground pests. On the other hand, the biological organic fertilizer for preventing and controlling underground pests of the present invention also has oil-blight type organic fertilizer, which can induce an increase in the number of soil microorganism populations, so that the biological organic fertilizer for preventing and controlling underground pests of the present invention can not only prevent and control underground pests, but also increase the number of soil beneficial microbial community populations, and can also greatly reduce the application amount of chemical pesticides, reduce environmental pollution, and improve crop safety.

[0036] The application of the biological organic fertilizer for preventing and controlling underground pests of the present invention can prevent and control underground pests by applying the biological organic fertilizer as a base fertilizer during the plant planting process, and can also increase the number of beneficial microbial communities in the soil, and can also greatly reduce the application amount of chemical pesticides, reduce environmental pollution, and improve crop safety. For example, the biological organic fertilizer for preventing and controlling underground pests of the present invention is used in the tobacco planting process, and can effectively prevent and control tobacco underground pests grubs, which can greatly reduce the application amount of chemical pesticides, reduce environmental pollution, and improve crop safety.

[0037] That is, the biological organic fertilizer for controlling underground pests, the preparation method and the application of the present invention solve the technical problems of the use of chemical pesticides for controlling pests in the prior art, resulting in pesticide residues and environmental pollution. DETAILED DESCRIPTION

[0038] To make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0039] The present invention will be further described below in conjunction with Examples 1 and 2.

[0040] Example 1

[0041] This example describes in detail the method for screening the biocontrol agent of the present invention.

[0042] 1. Materials and methods

[0043] 1.1 Materials

[0044] Biocontrol agents: Bacillus subtilis WSWFJ44, Beauveria bassiana WSWFJ49, Metarhizium anisopliae WSWFJ48.

[0045] Test insects: white grubs (collected from the test site of Yunnan Microbial Fermentation Engineering Research Center Co., Ltd.).

[0046] Culture medium: PDA medium (200 g potato, 20 g glucose, 1 L water), LB liquid medium (5 g yeast extract powder, 10 g bacteriological peptone, 5 g sodium chloride, 1 L water).

[0047] 1.2 Methods

[0048] 1.2.1 After activating the test insecticide, culture it for 3-5 days and prepare a bacterial suspension for later use.

[0049] 1.2.2 The underground pest grubs were tested indoors by the insect immersion method. The grubs were placed in the prepared agent for 3-5 seconds and then taken out and placed in a culture dish. Ten grubs were placed in each culture dish, and each treatment was repeated 3 times. The experimental settings were:

[0050] a. Treatment of Bacillus subtilis WSWFJ44 bacterial suspension stock solution;

[0051] b. Treatment of Beauveria bassiana WSWFJ49 bacterial suspension stock solution;

[0052] c. Treatment of the stock solution of Metarhizium anisopliae WSWFJ48 bacterial suspension;

[0053] d. Treatment with 800 times of highly effective cypermethrin;

[0054] e. The fermentation broth of Bacillus subtilis WSWFJ44, Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 was mixed at a ratio of 2:0.5:0.5;

[0055] f. Clean water treatment (blank control).

[0056] 1.2.3 The mortality rate of grubs in the culture dish was statistically recorded 1 day, 5 days, 7 days and 10 days after treatment, and the mortality rate was calculated according to the following formula.

[0057]

[0058] 2. Results Analysis

[0059] The statistical results of the average mortality of grubs treated with different microbial agents are shown in Table 1. As shown in Table 1, treatment a began to kill grubs on the 5th day, with a mortality rate of 36.67%; treatment b began to kill grubs on the 7th day, with a mortality rate of 70% on the 7th day; treatment c had a significant lethal effect on grubs on the 5th day, with a mortality rate of 40%; treatment d began to kill grubs 1 day after treatment, with a mortality rate of 33.33% on the 1st day; treatment e had a significant lethal effect on grubs on the 5th day, with a mortality rate of 53.33%; and there was no significant death of grubs in the blank control of treatment f. After statistics, it was found that on the 10th day, except for the blank control of treatment f where no grubs died, the mortality rate of treatment a was 80%, and the mortality rates of the other treatments reached 100%, indicating that the screened biocontrol insecticides Bacillus subtilis WSWFJ44, Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 all had obvious lethal effects on grubs.

[0060] Table 1 Statistical results of average mortality of white grubs treated with different microbial agents

[0061]

[0062]

[0063] Note: The above measurement results are the average mortality rate calculated three times.

[0064] Example 2

[0065] This example describes in detail the outdoor antagonism test of the biological organic fertilizer for preventing and controlling underground pests of the present invention on tobacco underground pests grubs and cutworms.

[0066] 1. Purpose of the test

[0067] To verify the control effect of biological organic fertilizer for underground pests obtained by mixing bio-insecticides Bacillus subtilis WSWFJ44, Beauveria bassiana WSWFJ49, Metarhizium anisopliae WSWFJ48 with oil-blight organic fertilizer on underground pests such as white grubs and cutworms.

[0068] 2. Materials and methods

[0069] 2.1 Materials

[0070] Organic fertilizers for testing: oil cake organic fertilizer with added pesticides and fungicides, and ordinary organic fertilizer.

[0071] Test subjects: tobacco underground pests white grubs and cutworms.

[0072] Test variety: Yunyan 97.

[0073] Experimental locations: Mafang Village, Yuezhou Town, Qilin District, Qujing City and Dapo Township, Zhanyi District, Qujing City.

[0074] 2.2 Methods

[0075] Before the experiment, the population density of grubs in the soil of the two test plots was counted, mainly by digging and sampling at 5 points, each with an area of ​​50cm*50cm (2500cm 2 ) The digging depth is generally 30cm, and the number of grubs in the excavated soil samples is carefully counted and recorded. The results are as follows:

[0076] Table 2 Survey results of population density of white grubs and cutworms in soil of test plots

[0077] Test point <![CDATA[The insect population density in the soil of the experimental plot (number / m 2 )]]> Qilin Yuezhou 1 Zhanyi Dapo 2

[0078] The bio-organic fertilizer and common organic fertilizer were applied at the local application rate of 80 kg / mu, and no fertilizer treatment was set as a control. The experimental treatments were:

[0079] A. Apply 80kg / mu of bio-organic fertilizer with biocontrol agents added;

[0080] B. Apply ordinary organic fertilizer 80kg / mu;

[0081] C. No fertilizer.

[0082] Each treatment was repeated three times, and each plot had an area of ​​100 m 2 , about 150 tobacco plants, base fertilizer is applied before tobacco transplanting, and the water and fertilizer management in the later stage is consistent.

[0083] The survey method is the same after transplanting until harvesting and curing. After harvesting and curing, root extraction survey will be conducted in each plot. 20 plants will be randomly surveyed in each plot, and the rate of damaged plants and control effect will be calculated according to the following formula. The formula is as follows:

[0084]

[0085]

[0086] 3. Results Analysis

[0087] 3.1. Field test results of biological organic fertilizer on grubs in Qilin Yuezhou, Qujing City

[0088] According to the statistical results in Table 3, at the Qilin Yuezhou test site in Qujing City, the application of bio-organic fertilizer with bio-control insecticides showed a statistically significant rate of 3.33% of plants damaged before harvesting, and a control effect of 87.5% on underground pests, grubs and cutworms; while the application of ordinary organic fertilizer treatment group, the rate of damaged plants was 13.33%, and the control effect was 50%; and the rate of damaged plants in the non-fertilization treatment group reached 26.67%. This shows that the application of bio-organic fertilizer has a significant inhibitory effect on underground pests, grubs and cutworms, and the application of ordinary organic fertilizer also has an inhibitory effect on grubs and cutworms, but the effect is not as good as the inhibitory effect of bio-organic fertilizer with bio-control agents.

[0089] Table 3 Statistical results of the control effect of Qilin Yuezhou biological organic fertilizer on underground pests and white grubs

[0090] Treatment Group Total number of plants surveyed Number of damaged plants / plant Damaged plant rate / % Control effect / % A 60 2 3.33 87.50 B 60 8 13.33 50.00 C 60 16 26.67 /

[0091] 3.2 Field test results of biological organic fertilizer on grubs in Dapo, Zhanyi, Qujing

[0092] According to the statistical results in Table 4, at the test site of Zhanyi Dapo in Qujing City, the application of bio-organic fertilizer with biological control insecticides showed a 5% rate of damaged plants before harvesting, and an 80% control effect on underground pests, grubs and cutworms; while the application of ordinary organic fertilizer treatment group, the rate of damaged plants was 15%, and the control effect was 40%; and the rate of damaged plants in the non-fertilization treatment group reached 25%. This shows that the application of bio-organic fertilizer has a significant inhibitory effect on underground pests, grubs and cutworms, and the application of ordinary organic fertilizer also has an inhibitory effect on grubs and cutworms, but the effect is only half of that of the bio-organic fertilizer with biological control agents.

[0093] Table 4 Statistical results of the control effect of bio-organic fertilizer on underground pests and white grubs in Zhanyi Dapo

[0094] Treatment Group Total number of plants surveyed Number of damaged plants / plant Damaged plant rate / % Control effect / % A 60 3 5.00 80.00 B 60 9 15.00 40.00 C 60 15 25.00 /

[0095] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A method for preparing a biological organic fertilizer for preventing and controlling underground pests, characterized in that: The steps include: preparing Bacillus subtilis WSWFJ44 bacterial powder, and preparing spore powder of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48; Preparation of oil-based organic fertilizer by fermentation; The prepared Bacillus subtilis WSWFJ44 spore powder, Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 spore powder are compounded with oil-blight type organic fertilizer to prepare biological organic fertilizer for preventing and controlling underground pests; Among them, the amount of effective live bacteria in the powder of Bacillus subtilis WSWFJ44 is not less than 100 billion / gram, and the amount of effective live bacteria of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 in the spore powder of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 is not less than 10 billion / gram; The deposit registration number of the Bacillus subtilis WSWFJ44 is CGMCC No. 20144, the deposit registration number of the Beauveria sp. WSWFJ49 is CGMCC No. 20213, and the deposit registration number of the Metarhizium sp. WSWFJ48 is CGMCC No. 19926.

2. The method for preparing the biological organic fertilizer for preventing and controlling underground pests according to claim 1, characterized in that, During the compounding process, the weight ratio of Bacillus subtilis WSWFJ44 powder, Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 spore powder is 2:0.5:0.

5.

3. The preparation method of the biological organic fertilizer for preventing and controlling underground pests according to claim 1 or 2, characterized in that, The preparation process of Bacillus subtilis WSWFJ44 bacterial powder includes the following steps: Activation of strains: Activate the Bacillus subtilis WSWFJ44 strain stored at -80°C on LB solid medium for 12h to 16h to obtain an activated strain; Preparation of seed solution: Use a sterile inoculation loop to scrape a loop of activated strain and inoculate it into 50 ml LB liquid culture medium. Cultivate at a temperature of 36.5°C-37.5°C and a rotation speed of 160 rpm / min for 24 hours to obtain seed solution. Preparation of fermentation liquid: The obtained seed liquid was inoculated into the workshop fermentation medium according to the ratio of the strain to the culture liquid volume of 1:120, and cultured at a temperature of 36.5°C-37.5°C and a rotation speed of 120 rpm / min for 24h-36h to obtain the fermentation liquid, which is the Bacillus subtilis WSWFJ44 bacterial suspension, and the effective viable bacterial count of the Bacillus subtilis WSWFJ44 bacterial suspension is 20.0×10 8 cfu / g or more; The obtained fermentation liquid is centrifuged and then an adsorption protective agent is added, and then the fermentation liquid with the adsorption protective agent added is transferred to a powder spraying device to obtain Bacillus subtilis WSWFJ44 bacterial powder, and the amount of effective live bacteria in the Bacillus subtilis WSWFJ44 bacterial powder is not less than 100 billion / gram.

4. The method for preparing the biological organic fertilizer for preventing and controlling underground pests according to claim 3, characterized in that, During the activation of bacterial strains, LB solid medium included the following components at the following concentrations: bacteriological peptone 10 g / L, yeast extract powder 5 g / L, sodium chloride 10 g / L, and agar powder 24 g / L; During the preparation of the seed solution, the LB liquid culture medium included the following components at the following concentrations: bacteriological peptone 10 g / L, yeast extract powder 5 g / L, and sodium chloride 10 g / L.

5. The preparation method of the biological organic fertilizer for preventing and controlling underground pests according to claim 3, characterized in that, During the preparation of the fermentation liquid, the fermentation medium includes the following ingredients in percentage by mass: 1.0%-1.6% soybean powder, 0.1%-0.5% sucrose, 0.2%-0.6% peptone, 0.2%-0.6% corn flour, 0.3%-0.9% calcium carbonate, 0.1%-0.3% fish meal, 0.01%-0.08% ammonium sulfate, 0.02%-0.05% magnesium sulfate, 0.01%-0.04% potassium dihydrogen phosphate, 0.02%-0.05% dipotassium hydrogen phosphate, 0.01%-0.04% manganese sulfate, 0.01%-0.04% sodium hydroxide, 0.02%-0.03% sodium chloride, 0.5%-1.5% defoaming agent, and the balance is water, and the pH is adjusted to 6.8-7.5 with hydrochloric acid and sodium hydroxide.

6. The preparation method of the biological organic fertilizer for controlling underground pests according to claim 1 or 2, characterized in that, The preparation process of spore powder of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 comprises the following steps: Preparation of seed solutions of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48: Activate the preserved Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 on PDA solid culture medium, inoculate into 200 mL PDA liquid culture medium after activation, place on a constant temperature shaker at 28°C and 180 r / min for culturing for 72 h, then inoculate the cultured bacterial suspension into 1000 mL PDA liquid culture medium at 1% inoculation amount, place on a constant temperature shaker at 28°C and 180 r / min for propagation for 72 h, to obtain seed solutions of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48; Preparation of fermentation broth of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48: Add 600L of special fermentation medium to a fermentation tank with a volume of 1000L, add seed liquid of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 to the fermentation tank, and simmer at a ventilation volume of 35m 3 / h, temperature 28 ° C and rotation speed 160r / min, and fermented for 48h-72h to obtain the fermentation liquid of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48; Solid fermentation: add the fermentation liquid of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48 to the solid culture medium at a weight ratio of 1:10, add water to adjust the water content of the fermentation material to 60%-80%, mix evenly in a blender, spread the fermentation material in the fermentation tank after mixing, the height of the mixed material is 10cm-15cm, cover the top of the fermentation tank with a black shade net, and keep the temperature at 28℃-30℃ and the ventilation volume at 5m 3 / h conditions for 60 h, and after 60 h, open the black shade net at 30-35 ° C and a ventilation volume of 5m 3 / h conditions for fermentation for 36 hours, after 36 hours, add the mixed material into a drying barrel, and dry it at a temperature of 50℃-55℃ until the water content of the material drops to 10%-15%, and then grind it into powder to obtain spore powder of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48, and the amount of effective live bacteria in the spore powder is not less than 10 billion / g.

7. The method for preparing the biological organic fertilizer for controlling underground pests according to claim 6, characterized in that: During the preparation of the seed solution of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48, the PDA liquid culture medium included the following components in concentrations: 200 g / L potato and 20 g / L glucose; the PDA solid culture medium included the following components in concentrations: 200 g / L potato, 20 g / L glucose, and 24 g / L agar.

8. The method for preparing the biological organic fertilizer for controlling underground pests according to claim 6, characterized in that: During the preparation of fermentation broth of Beauveria bassiana WSWFJ49 and Metarhizium anisopliae WSWFJ48, the special fermentation medium includes the following components in concentrations: yeast extract 3g / L-5g / L, peptone 3g / L-5g / L, corn steep liquor powder 8g / L-10g / L, corn starch 3g / L-5g / L, dipotassium hydrogen phosphate 0.2g / L-0.5g / L, potassium dihydrogen phosphate 0.1g / L-0.3g / L, glucose 10g / L-15g / L and molasses powder 1g / L-10g / L, and the pH is adjusted to 6.5-7.0; During the solid fermentation process, the solid culture medium includes the following components in parts by weight: 50-100 parts of corn flour, 300-400 parts of oil cake, 300-400 parts of peat, 100-200 parts of wheat bran, 100-200 parts of rice husk, and 50-100 parts of soybean powder, and water is added to adjust the moisture content to 60%-80%.

9. A biological organic fertilizer for preventing and controlling underground pests, characterized in that: It is prepared by the preparation method according to any one of claims 1 to 8.

10. The use of the biological organic fertilizer for preventing and controlling underground pests according to claim 9, characterized in that: During the plant planting process, the bio-organic fertilizer is applied as base fertilizer.

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