Sample slide preparation equipment and methods

Abstract

A sample slide preparation device and method are disclosed, wherein the sample transport device transports samples including trace blood samples and normal blood samples. A trace blood sample mixing device drives the movement of the trace blood sample to mix it. A normal blood sample mixing device drives the movement of the normal blood sample to mix it. A control unit determines whether the sample slide preparation device operates in normal blood mode or trace blood mode based on sample type information, and selects different methods to complete sample mixing, sample collection, and movement according to the different modes, finally preparing a sample slide for subsequent detection and analysis.

Description

Technical Field

[0001] This application relates to the field of medical devices, specifically to a sample slide preparation device and method. Background Technology

[0002] Sample slides are frequently used in medical testing and diagnostics. For example, when the test parameters of a blood sample are outside the normal range and morphological re-examination is required for confirmation, a sample slide (i.e., a blood smear) needs to be prepared for microscopic examination. Blood smears can be prepared manually or using an automated slide pusher.

[0003] In clinical diagnosis, there are currently two methods of blood collection: venous blood collection and capillary blood collection. Venous blood collection involves collecting a larger volume of blood (≥1mL) and is generally suitable for adult patients. However, for infants, children, or critically ill patients, it is sometimes difficult to collect blood via venous blood collection. In such cases, capillary blood collection is often used, but the amount of blood that can be collected is relatively small (most often ≤80μL).

[0004] During blood collection, blood collection tubes containing anticoagulants are usually used to prevent blood clotting. Blood is composed of blood cells and plasma. Due to the different specific gravities of blood cells and blood samples, anticoagulated blood will separate into layers after standing for a period of time. Therefore, the blood sample must be thoroughly mixed before measurement; otherwise, the measurement results will be significantly inaccurate.

[0005] For trace blood samples, due to the small sample volume (usually ≤80uL) and poor sample flowability, they are typically mixed manually by shaking before being loaded onto the instrument for slide preparation. However, this manual mixing method is inefficient and cannot meet the growing demand for trace blood sample testing. Summary of the Invention

[0006] This application mainly provides a sample slide preparation device and a sample slide preparation method to achieve mechanized slide preparation for trace amounts of blood samples.

[0007] Based on the above objectives, one embodiment of this application provides a sample slide preparation device. The operating modes of the sample slide preparation device include at least a normal blood volume mode and a micro-blood volume mode. The sample slide preparation device includes:

[0008] A sample transport device for transporting sample containers containing samples, wherein the samples transported by the sample transport device include trace blood samples and normal blood samples, a first sample container for storing the trace blood samples and a second sample container for storing the normal blood samples;

[0009] A micro-blood sample mixing device, wherein the micro-blood sample mixing device is capable of driving the first sample container to move in order to mix the micro-blood sample.

[0010] A constant blood sample mixing device, wherein the constant blood sample mixing device is capable of driving the second sample container to move in order to mix the constant blood sample.

[0011] A slide supply device for supplying blank slides;

[0012] A sample transfer device is used to collect the mixed sample from the first sample container and the second sample container and transfer the collected sample onto the blank glass slide.

[0013] A slide-pushing device is used to flatten the sample on the blank slide to form a sample slide;

[0014] The control unit is connected to the sample transport device, the micro-blood sample mixing device, the macro-blood sample mixing device, the slide supply device, the sample transfer device, and the slide pusher via signal connection, and is used to control the operation of the sample transport device, the micro-blood sample mixing device, the macro-blood sample mixing device, the slide supply device, the sample transfer device, and the slide pusher, wherein:

[0015] The control unit determines the operating mode of the sample slide preparation equipment based on the sample type information;

[0016] When the sample slide preparation equipment is in the micro-blood mode, the control unit controls the micro-blood sample mixing device to mix the micro-blood sample in the first sample container, controls the sample transfer device to collect the micro-blood sample in the first sample container after it has been mixed by the micro-blood sample mixing device, and transfers the collected micro-blood sample to the blank slide. Then, the control unit controls the slide pusher to flatten the micro-blood sample on the blank slide to form a sample slide.

[0017] When the sample slide preparation equipment is in constant blood mode, the control unit controls the constant blood sample mixing device to mix the constant blood sample in the second sample container, controls the sample transfer device to collect the constant blood sample in the second sample container after it has been mixed by the constant blood sample mixing device, and transfers the collected constant blood sample to the blank slide. Then, the control unit controls the slide pusher to flatten the constant blood sample on the blank slide to form a sample slide.

[0018] To achieve the above objectives, one embodiment of this application provides a method for preparing a sample slide, which is applied in a sample slide preparation device. The sample slide preparation device includes: a sample transport device, a slide supply device, an identification device, a sample transfer device, a micro-volume blood sample mixing device, a macro-volume blood sample mixing device, and a slide pushing device.

[0019] The sample transport device is used to transport a first sample container containing a trace amount of blood sample and a second sample container containing a constant amount of blood sample.

[0020] The slide supply device supplies blank glass slides;

[0021] The identification device acquires sample type information of the sample transported by the sample transport device;

[0022] When the sample type information obtained by the identification device indicates that the sample transported by the sample transport device is a trace blood sample, the first sample container containing the trace blood sample is transferred to the trace blood sample mixing device, which mixes the sample in the first sample container. The sample transfer device collects the trace blood sample after it has been mixed by the trace blood sample mixing device and transfers the collected trace blood sample to the blank slide. The slide pusher flattens the trace blood sample on the blank slide to form a sample slide.

[0023] When the sample type information obtained by the identification device indicates that the sample transported by the sample transport device is a constant blood sample, the second sample container containing the constant blood sample is transferred to the constant blood sample mixing device, which mixes the constant blood sample in the second sample container. The sample transfer device collects the constant blood sample after it has been mixed by the constant blood sample mixing device and transfers the collected constant blood sample to the blank slide. The slide pusher flattens the constant blood sample on the blank slide to form a sample slide.

[0024] To achieve the above objectives, one embodiment of this application provides a sample slide preparation apparatus, comprising:

[0025] A sample transport device for transporting a sample container containing a sample, the sample transport device transporting a sample including a trace blood sample, the sample container including a first sample container for storing the trace blood sample;

[0026] A micro-blood sample mixing device is used to drive the first sample container to move in order to mix the micro-blood sample.

[0027] A slide supply device for supplying blank slides;

[0028] A sample transfer device is used to collect a trace blood sample from a first sample container after it has been mixed by the trace blood sample mixing device, and to transfer the collected trace blood sample onto the blank glass slide.

[0029] A slide-pushing device is used to flatten a small amount of blood sample on the blank slide to form a sample slide;

[0030] The control unit is connected to the sample transport device, the micro-blood sample mixing device, the slide supply device, the sample transfer device, and the slide pusher via signal connection, and is used to control the operation of the sample transport device, the micro-blood sample mixing device, the slide supply device, the sample transfer device, and the slide pusher.

[0031] According to the sample slide preparation equipment and method of the above embodiments, the samples transported by the sample transport device include trace blood samples and normal blood samples. The trace blood sample mixing device can drive the movement of the trace blood sample to mix it. The normal blood sample mixing device can drive the movement of the normal blood sample to mix it. The control unit determines whether the sample slide preparation equipment operates in normal blood mode or trace blood mode based on sample type information, and selects different methods to complete sample mixing, sample collection, and movement according to different modes, finally preparing a sample slide for subsequent detection and analysis. Attached Figure Description

[0032] Figure 1 This is a schematic diagram of the structure of a sample slide fabrication device in one embodiment of this application;

[0033] Figure 2 This is a schematic diagram of the structure of the first sample container (micro-blood sample storage container) in one embodiment of this application;

[0034] Figure 3 This is a schematic diagram of the cap structure of the first sample container in one embodiment of this application;

[0035] Figure 4 This is a schematic diagram of the structure of the second sample container (constant blood sample storage container) in one embodiment of this application;

[0036] Figure 5 This is a schematic diagram of the sample holder structure in one embodiment of this application;

[0037] Figure 6 This is an exploded view of the sampling device in one embodiment of this application;

[0038] Figure 7 This is a schematic diagram of the structure of a micro-blood sample mixing device in one embodiment of this application;

[0039] Figure 8 This is a schematic diagram of the structure of a constant blood sample mixing device in one embodiment of this application;

[0040] Figure 9 This is a schematic diagram of the sample transfer device in one embodiment of this application;

[0041] Figure 10 This is a schematic diagram of the structure of the first sampling needle in one embodiment of this application;

[0042] Figure 11 This is an enlarged view of the needle tip portion of the first sampling needle in one embodiment of this application;

[0043] Figure 12 This is a schematic diagram of the structure of the second sampling needle in one embodiment of this application;

[0044] Figure 13 This is an enlarged view of the tip portion of the second sampling needle in one embodiment of this application;

[0045] Figure 14 This is a schematic diagram of the first sampling needle dropping a sample onto a blank glass slide in one embodiment of this application;

[0046] Figure 15 This is a schematic diagram of the structure of the sample container rotating device in one embodiment of this application. Detailed Implementation

[0047] The present invention will now be described in further detail with reference to specific embodiments and accompanying drawings. Similar elements in different embodiments are referred to by associated similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of this application. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, certain operations related to this application are not shown or described in the specification. This is to avoid obscuring the core parts of this application with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.

[0048] Furthermore, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can be rearranged or adjusted in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and drawings are only for the clear description of a particular embodiment and do not imply a necessary order, unless otherwise stated that a particular order must be followed.

[0049] The serial numbers assigned to components in this document, such as "first" and "second," are used only to distinguish the described objects and have no sequential or technical meaning. The terms "connection" and "linkage" used in this application, unless otherwise specified, include both direct and indirect connections (linkages).

[0050] This application provides a sample slide preparation device, such as an automatic slide preparation machine. This device can mechanize and batch-produce sample slides (blood smears), especially for batch production using trace amounts of blood samples. Of course, depending on actual needs, in some embodiments, this device can be used only for preparing sample slides from trace amounts of blood samples, or it can be used for preparing sample slides from both trace and normal amounts of blood samples. The sample described in this embodiment can be a whole blood sample or a pre-diluted sample. The sample container can contain trace amounts of capillary blood or venous blood. A whole blood sample means a blood sample that has not been diluted and is directly collected from a human or animal. A trace blood sample refers to a whole blood sample with a volume ≤250 μL. This trace blood sample may be capillary blood, venous blood, or blood from other sites. A normal blood sample refers to a whole blood sample with a volume ≥1 mL.

[0051] Please refer to Figure 1 In one embodiment, the sample slide preparation device 1 includes a sample transport device 2, a micro-volume blood sample mixing device 3, a macro-volume blood sample mixing device 4, a slide supply device (including a slide supply device 6 and a first slide transport device 7), a sample transfer device 5, a slide pusher device 9, and a control unit (not shown in the figure). Of course, the sample slide preparation device 1 may also include other components, such as a flipping device (not shown in the figure), a printing device 8 for printing sample information on a blank slide 100, a staining device 10 containing dye, a second slide transport device 11, etc., wherein the dye in the staining device 10 stains the cells on the sample slide. These components can be referenced from existing sample slide preparation devices 1.

[0052] The sample transport device 2 is used to transport sample containers containing samples. The samples transported by the sample transport device 2 include trace blood samples and / or normal blood samples. Please refer to the relevant documentation depending on the type of blood sample (e.g., determining whether it is a trace or normal blood sample). Figure 2-4 The sample container (such as a blood sample tube) can be divided into a first sample container 91 and a second sample container 92. The first sample container 91 is used to store a small amount of blood sample, and the second sample container 92 is used to store a large amount of blood sample. The small amount blood sample mixing device 3 can drive the first sample container 91 to move and mix the small amount of blood sample. The large amount blood sample mixing device 4 can drive the second sample container 92 to move and mix the large amount of blood sample. The slide supply device is used to supply a blank slide 100 to provide a carrier for the sample. The sample transfer device 5 is used to collect the mixed sample in the first sample container 91 and the second sample container 92 and transfer the collected sample to the blank slide 100. The slide pusher 9 is used to flatten the sample on the blank slide 100 to form a sample slide (i.e., a blood smear).

[0053] The control unit is signal-connected to the sample transport device 2, the micro-blood sample mixing device 3, the macro-blood sample mixing device 4, the slide supply device, the sample transfer device 5, the slide pusher device 9, and other related devices to control their operation. The control unit can employ various structures capable of data transmission and reception, and data processing, such as controllers or other structures with processors and memory.

[0054] The sample slide preparation device 1 shown in this embodiment can mix both trace amounts of blood samples and normal amounts of blood samples. Therefore, the operating modes of the sample slide preparation device 1 include at least a normal blood mode and a trace blood mode. The control unit determines the operating mode of the sample slide preparation device 1 based on the sample type information.

[0055] For example, when the sample slide preparation device 1 is in the micro-blood mode, the control unit controls the micro-blood sample mixing device 3 to mix the micro-blood sample in the first sample container 91, controls the sample transfer device 5 to collect the micro-blood sample in the first sample container 91 after being mixed by the micro-blood sample mixing device 3, and transfers the collected micro-blood sample to the blank slide 100. Then, the control unit controls the slide pusher 9 to push the micro-blood sample on the blank slide 100 flat to form a sample slide.

[0056] When the sample slide preparation device 1 is in the constant blood mode, the control unit controls the constant blood sample mixing device 4 to mix the constant blood sample in the second sample container 92, controls the sample transfer device 5 to collect the constant blood sample in the second sample container 92 after mixing by the constant blood sample mixing device 4, and transfers the collected constant blood sample to the blank slide 100. Then, the control unit controls the slide pusher 9 to push the constant blood sample on the blank slide 100 flat to form a sample slide.

[0057] Of course, if only batch preparation of slides from trace amounts of blood samples is required, only the trace blood sample mixing device 3 can be installed, while the regular blood sample mixing device 4 can be omitted. When preparing slides from trace amounts of blood samples, the slide preparation equipment 1 can simply run in trace blood mode.

[0058] The following provides a more detailed description of the structures of some embodiments of the various devices.

[0059] Please refer to Figure 2 and 3In one embodiment, the first sample container 91 includes a cap 911 and a tube 912. The tube 912 has an inner cavity for receiving samples. The bottom 912a of the inner cavity of the tube 912 is a certain distance from the bottom of the tube. The inner cavity of the first sample container 91 has a small volume and is used to hold trace amounts of blood samples, such as small amounts of capillary blood samples. Compared to the second sample container 92, the bottom 912a of the inner cavity of the first sample container 91 is higher, making it easier to hold trace amounts of blood samples at a higher position, facilitating sample aspiration by the sampling needle of the sample transfer device 5. At least the middle region of the cap 911 is made of a thin rubber material and has a through-cut cross slit 911a (as shown in the figure). The sampling needle of the sample transfer device 5 can easily enter the inner cavity of the first sample container 91 to aspirate samples without piercing the cap 911 through the through-cut cross slit 911a in the middle of the cap 911.

[0060] Please refer to Figure 4 In one embodiment, the second sample container 92 also includes a cap 921 and a tube 922. The tube 912 also has an inner cavity for receiving samples. The difference between the tube 92 and the first sample container 91 is that the bottom 922a of the inner cavity of the second sample container 92 is located at the bottom of the tube. The inner cavity of the second sample container 92 has a larger volume to accommodate constant blood samples, such as large-volume venous blood samples. At least the middle region of the cap 911 is made of rubber material. The rubber material is thick and has no slits, so the sampling needle of the sample transfer device 5 needs to pierce the cap 921 to enter the inner cavity of the second sample container 92 to aspirate the sample.

[0061] Sample transport device 2 transports samples in batches via sample rack 90. ​​Please refer to [reference needed]. Figure 5 As one example, the sample holder 90 is provided with fixing holes 901, and a sample container (such as a blood sample tube) is placed in the fixing holes 901, either in a movable or fixed position. Each fixing hole 901 is provided with an opening 902. A portion of the sample container can be exposed through the opening 902, allowing observation of the contents of the sample container.

[0062] In one embodiment, the sample rack 90 may also be provided with a label setting area 903 for setting sample rack labels. The sample rack label may include, but is not limited to, barcodes, QR codes, and other features capable of containing sample rack-related information. When needed, these sample rack labels can be identified by an identification device. Similarly, similar sample labels can be set on sample containers to store sample-related information.

[0063] Please refer to Figure 1 and 6In one embodiment, the sample transport device 2 is used to transport a sample rack 90 on which a first sample container 91 and / or a second sample container 92 are fixed. The first sample container 91 and / or the second sample container 92 fixed on the sample rack 90 are transported one by one to the mixing position and the sampling position.

[0064] Samples located at the mixing position (including trace blood samples and constant blood samples) can be mixed by mixing devices (including trace blood sample mixing device 3 and constant blood sample mixing device 4). Mixing of samples can be achieved either by moving the corresponding trace blood sample mixing device 3 and constant blood sample mixing device 4 to the mixing position, or by transferring the sample located at the mixing position to the corresponding trace blood sample mixing device 3 and constant blood sample mixing device 4 for mixing.

[0065] The sample transfer device 5 collects the sample (which has been mixed) located at the sampling position and transfers the collected sample to the blank glass slide 100, and then performs subsequent operations such as slide pushing, drying, staining, etc.

[0066] Further, please refer to Figure 1 and 6 In a more specific embodiment, the sample transport device 2 includes a sample rack support component 21, a sample rack feeding module 22, a sample rack lateral transport module 23, and a sample rack delivery module 24. The sample rack support component 21 supports the sample rack 90. ​​The sample rack feeding module 22, the sample rack lateral transport module 23, and the sample rack delivery module 24 can be mounted on the sample rack support component 21 or located elsewhere, for example... Figure 6 As shown, the sample rack feeding module 22, the sample rack lateral transport module 23, and the sample rack delivery module 24 are all mounted on a support located below the sample rack support component 21. The sample rack feeding module 22, the sample rack lateral transport module 23, and the sample rack delivery module 24 may have corresponding output components, such as levers, push plates, push claws, hooks, and other motion output structures capable of moving the sample rack 90 in a specified direction. These motion output structures can extend onto the sample rack support component 21 and move under the drive of a power component, which includes, but is not limited to, motors, cylinders, hydraulic cylinders, electromagnets, etc.

[0067] Please continue to refer to this. Figure 6The sample rack support component 21 includes a pre-analysis sample rack storage area 211, a post-analysis sample rack storage area 212, and a sample analysis area 213. The pre-analysis sample rack storage area 211 holds several sample racks 90 fixed with sample containers (which can be first sample containers 91 or second sample containers 92) holding pre-analysis samples. The post-analysis sample rack storage area 212 holds several sample racks 90 fixed with sample containers holding post-analysis samples. The sample analysis area 213 is located between the pre-analysis sample rack storage area 211 and the post-analysis sample rack storage area 212. The pre-analysis sample rack storage area 211 has a sample rack feeding turning area 211a, and the post-analysis sample rack storage area 212 has a sample rack output turning area 212a. The mixing and sampling positions can be located within the sample analysis area 213.

[0068] The sample rack 90 moves on the sample rack support component 21. The sample rack feeding module 22 drives the sample rack 90, located in the pre-analytical sample rack storage area 211, to move along the Y2 direction to the sample rack delivery turning area 212a. Next, the sample rack lateral transport module 23 drives the sample rack, located in the sample rack delivery turning area 212a, to move along the X1 direction to the mixing position, sampling position, etc. After the mixing and sampling operations are completed, the sample rack lateral transport module 23 continues to drive the sample rack 90 to the sample rack delivery turning area 212a. Afterward, the sample rack delivery module 24 can transport the sample rack 90 in the sample rack delivery turning area 212a along the Y1 direction to the post-analytical sample rack storage area 212 for retrieval and temporary storage.

[0069] Of course, the above is only one example of the sample transport device 2. The sample transport device 2 can also adopt other structures that can realize sample rack or sample input and output, such as the structures of various sample transport devices in existing blood smear preparation instruments.

[0070] Furthermore, when mixing a trace amount of blood sample, the first sample container 91 can be moved by controlling the trace amount of blood sample mixing device 3 to mix the trace amount of blood sample in the first sample container 91. Of course, the first sample container 91 can also be kept stationary, and the sample can be mixed by stirring or other methods.

[0071] Since the blood volume of a trace blood sample is small, in order to minimize the loss of the trace blood sample caused by the mixing action, in one embodiment, during the movement of the trace blood sample mixing device 3 driving the first sample container 91, the bottom of the first sample container 91 is controlled to be kept lower than the opening of the first sample container 91, so that the blood sample does not spill out of the first sample container 91 or stick to the cap 911, and to reduce damage to the blood components in the blood sample, thereby avoiding the loss of the trace blood sample and making it easier for the sample transfer device 5 to collect the trace blood sample.

[0072] For example, please refer to Figure 7 In one exemplary configuration, the micro-blood sample mixing device 3 includes a container holder 31 and a first drive assembly. The first drive assembly may be a motor 32 or other power component. The container holder 31 has a container mounting cavity 311 capable of holding a first sample container 91, which may be perforated or have other shapes. The motor 32 of the first drive assembly is connected to the container holder 31 in a transmission connection. When the sample slide preparation device 1 is in micro-blood mode, the motor 32 drives the first sample container 91 to rotate around the support point of the container holder 31. During rotation, the bottom of the first sample container 91 remains lower than the opening of the first sample container 91.

[0073] For more details, please refer to [link / reference]. Figure 7 In one embodiment, the micro-blood sample mixing device 3 includes a motor 32, a container holder 31, and a sensor 33. The motor 32 can be a stepper motor, a servo motor, or a DC motor. In this embodiment, the motor 32 is a stepper motor.

[0074] The container mounting base 31 is fixed to the rotating shaft of the motor 32 by screws or other suitable means. The motor 32 provides rotational driving force to the container mounting base 31, and the container mounting base 31 can rotate synchronously with the rotating shaft of the motor 32. The container mounting base 31 can be directly fixed to the rotating shaft of the motor 32, or it can be indirectly rotatably connected to the rotating shaft of the motor 32, such as through a synchronous belt.

[0075] In one embodiment, the container mounting base 31 can be set as part of the rotating shaft of the motor 32, that is, the rotating shaft of the motor 32 and the container mounting base 31 are integrally formed without the need for additional connection. This can solve the problem of the container mounting base 31 becoming loose during use, and can also further reduce the size of the device by eliminating the need for screw installation.

[0076] Sensor 33 is used to detect whether the container holder 31 rotates at a set speed and number of revolutions. The lower part of the container holder 31 is provided with a sensor blocking part 311 and a notch 312. Each time the container holder 31 rotates, the notch 312 passes through the sensing area of ​​the sensor 33 once, causing the sensor 33 to generate a state change of blocking → unblocking → blocking. The control unit can calculate the rotational speed of the container holder 31 by monitoring the period of the output signal of the sensor 33, and the number of rotations of the container holder 31 can be determined by monitoring the number of jumps in the output signal of the sensor 33. Those skilled in the art will understand that a sensor protrusion can also be provided at the lower part of the container holder 31, causing the sensor 33 to generate a state change of unblocking → blocking → unblocking to calculate the rotational speed.

[0077] The container mounting base 31 has a container mounting cavity 311 at its top, and the diameter of the cavity 311 is slightly larger than the outer diameter of the first sample container 91 to be placed inside. The center of the container mounting cavity 311 can be set to be non-coincident with the axis of rotation of the motor 32, which causes the first sample container 91 in the container mounting cavity 311 to rotate eccentrically. Of course, the center of the receiving hole 1121 can also be set to coincide with the axis of rotation of the motor 32, which causes the first sample container 91 in the receiving hole 1121 to rotate concentrically.

[0078] In one embodiment, the container mounting cavity 311 can be eccentrically positioned relative to the container fixing seat 31, with the eccentricity ranging from 0mm to 5mm, preferably from 1mm to 2mm. This eccentric positioning of the container mounting cavity 311 relative to the container fixing seat 31 allows the first sample container 91 within the container mounting cavity 311 to rotate eccentrically.

[0079] Before mixing, the first sample container 91 is placed into the container mounting cavity 311. Then, the control unit controls the motor 32 to drive the container fixing seat 31 to rotate the first sample container 91, so that the trace amount of blood sample in the first sample container 91 forms a vortex to achieve the purpose of mixing the blood sample.

[0080] Furthermore, the motor 32, acting as a power source, can drive the container holder 31 to rotate clockwise (forward) or counterclockwise (reverse). During the mixing of a small amount of blood sample, the container holder 31 drives the first sample container 91 containing the small amount of blood sample to rotate, generating mixing power through rotation. In this embodiment, the rotation direction of the container holder 31 can be counterclockwise (reverse), clockwise (forward), or alternating between clockwise and counterclockwise. This alternating clockwise and counterclockwise rotation allows the small amount of blood sample in the first sample container 91 to collide with each other, thereby improving the mixing effect.

[0081] Specifically, the rotation direction of the container fixing base 31 can be counterclockwise ( Figure 7 In the R1 direction, it can be clockwise ( Figure 7 (in the R2 direction), or rotate alternately counterclockwise and clockwise. Preferably, the container fixing seat 31 can rotate alternately counterclockwise and clockwise.

[0082] Of course, in addition to the structure shown above, the micro-blood sample mixing device 3 can also adopt other structures that can achieve mixing of micro-blood samples.

[0083] like Figure 8As shown, the constant blood sample mixing device 4 includes a gripper 41, motors 42, 43, and 44, and slide rails 45 and 46. The gripper 41 can move laterally along slide rail 45 in the Y1 or Y2 direction under the drive of motor 42. The gripper 41 can also move vertically along slide rail 46 in the Z1 or Z2 direction under the drive of motor 43, and the gripper 41 can also swing around an axis in the R1 or R2 direction under the drive of motor 44. Motors 42, 43, and 44 can be stepper motors.

[0084] The front end of the gripper 41 has a clamping piece capable of holding the second sample container 92. When the sample slide preparation device 1 is in the constant blood mode, the gripper 41 of the constant blood sample mixing device 4 can move along the Y1, Y2 and Z1, Z2 directions to pick up the second sample container 92 located at the mixing position from the sample holder 90, and mix the constant blood sample in the container by swinging around the axis in the R1, R2 directions in a reciprocating inverting manner. This reciprocating inverting manner can fully promote the movement of the constant blood sample in the second sample container 92, thereby improving the mixing effect. After the mixed second sample container 92 is returned to the sample transport device 2, the sample transport device 2 moves the second sample container 92 to the sampling position for the sample transfer device 5 to collect the mixed constant blood sample located at the sampling position.

[0085] Of course, this inverted mixing method is only one way to mix a constant blood sample. The constant blood sample mixing device 4 can also adopt other structures that can achieve this mixing function.

[0086] Furthermore, to save components and make the overall equipment more compact and miniaturized, in one embodiment, when the sample slide preparation device 1 is operating in micro-blood mode, the grippers of the constant blood sample mixing device 4 can also move along the Y1, Y2 and Z1, Z2 directions to transport the first sample container 91 located at the mixing position from the sample rack 90 to the container mounting cavity 311 of the micro-blood sample mixing device 3, and to transport the first sample container 91 from the container mounting cavity 311 of the micro-blood sample mixing device 3 to the sample rack 90. ​​Therefore, the constant blood sample mixing device 4 can act as a container transfer device for the first sample container 91 to transport the first sample container 91. Using the constant blood sample mixing device 4 as a container transfer device simplifies the overall structure, making the whole machine more compact and smaller, and more suitable for certain medical places with limited space.

[0087] Of course, in other embodiments, a separate container transfer device can also be provided, which is used to transfer the first sample container 91 between the sample transport device 2 and the micro-blood sample mixing device 3. When the sample slide preparation device 1 is in micro-blood mode, the container transfer device transfers the first sample container 91 from the sample transport device 2 to the micro-blood sample mixing device 3 for mixing. After mixing, the container transfer device transfers the first sample container 91 from the micro-blood sample mixing device 3 back to the sample transport device 2. When the mixed first sample container 91 is returned to the sample transport device 2, the sample transport device 2 moves the first sample container 91 to the sampling position so that the sample transfer device 5 can collect the mixed micro-blood sample located at the sampling position. The container transfer device can transfer the first sample container 91 by means of clamping, vacuum adsorption, magnetic adsorption, etc.

[0088] In one embodiment, the micro-blood sample mixing device 3 can be located on the side of the sample transport device 2 to shorten the travel distance of the first sample container 91 in the container transfer device, making it easier to control and transport the first sample container 91 back and forth.

[0089] Furthermore, in some embodiments, the sample slide preparation device 1 is capable of mixing both trace amounts of blood samples and large amounts of blood samples. Therefore, in some embodiments, the type of sample will be identified first, and then it will be determined which mode to perform.

[0090] In one embodiment, an identification device is further included, which is signal-connected to the control unit. The identification device acquires sample type information of the sample transported by the sample transport device 2 and sends the acquired sample type information to the control unit. The control unit determines whether to execute the constant blood mode or the micro-blood mode based on the received information.

[0091] The sample rack 90 for storing the first sample container 91 and the second sample container 92 can be configured with different shapes, or the first sample container 91 and the second sample container 92 can also be configured with different shapes. The identification device can determine sample type information by acquiring at least one of the sample rack type, the sample container type, and the sample quantity of the sample container transported by the sample transport device 2. For example, the identification device can use a barcode scanner, photoelectric sensor, image acquisition and comparison device, or other types of detection devices to detect and identify the sample rack type, the sample container type, and the sample quantity.

[0092] With this identification device, the sample slide preparation equipment can automatically identify samples and automatically execute the normal blood mode and micro blood mode based on the identification results, improving the automation efficiency of the equipment. It eliminates the need for manual control, reduces the number of manual operation steps for users, and avoids detection errors caused by improper manual operation.

[0093] In one embodiment, the sample slide preparation device 1 can distinguish whether the current sample is in the first sample container 91 or the second sample container 92 by obtaining the sample rack label information pasted on the sample rack 90, the label information of the first sample container 91 and the second sample container 92, and thus determine whether the sample is a trace blood sample or a normal blood sample.

[0094] In addition to the device automatically identifying the sample type information, in some embodiments, the user can also manually input the sample type information or through other data transmission terminals. For example, the type of sample to be mixed and related requirements can be directly input, and the device will determine whether to execute the constant blood mode or the micro blood mode based on this information.

[0095] For example, in one embodiment, the sample slide preparation device 1 further includes a receiving device. The receiving device is signal-connected to the control unit and is used to receive externally input instructions indicating sample type information, and to send the received instructions to the control unit. The control unit determines whether to execute the constant blood mode or the micro-blood mode based on the received information.

[0096] The receiving device can be a physical button, touch screen, voice recognition device, image recognition device, or wireless or wired information input device. Using this receiving device, the sample slide fabrication equipment 1 can receive external commands, which the user can then use to control the equipment. In particular, these commands are not limited to those manually entered by the user on the device. The receiving device can also automatically recognize external sound and image information (such as user gestures, facial recognition, etc.) through voice recognition and image recognition devices to complete command input. This allows users to input commands when they are unable to physically touch the device (e.g., when their hands are unavailable). Simultaneously, the receiving device can also receive information from other electronic devices via wireless or wired information input devices, thereby enabling remote control of the equipment even when the user is not physically present.

[0097] Further, please refer to Figure 15 The sample slide preparation equipment 1 may also include a sample container rotation device 14, which is used to rotate the first sample container 91 or the second sample container 92 placed on the sample rack 90 when the coded information affixed to the first sample container 91 or the second sample container 92 is not facing the barcode scanner 13 (or other identification device). The sample container rotation device 14 can rotate the first sample container 91 or the second sample container 92 on the sample rack 90 so that the side of the first sample container 91 or the second sample container 92 with the coded information affixed faces the barcode scanner 13, so that the barcode scanner 13 can complete the scanning.

[0098] Whether the device automatically identifies and judges the sample or receives external commands, when the identification result is a trace blood sample, the control unit controls the sample transfer device 5 to aspirate the trace blood sample from the first sample container 91; when the identification result is a normal blood sample, the control unit controls the sample transfer device 5 to aspirate the normal blood sample from the second sample container 92. In both normal and trace blood modes, the amount of blood aspirated by the sample transfer device 5 in the trace blood mode can be the same as or different from the amount of blood aspirated in the normal blood mode. For example, the amount of blood aspirated in the trace blood mode may be less than or the same as the amount of blood aspirated in the normal blood mode.

[0099] The sample transfer device 5 will be further described below.

[0100] The main function of the sample transfer device 5 is to transfer the corresponding trace or normal blood sample, allowing the mixed sample to adhere to the blank slide 100. The sample transfer device 5 typically performs sampling via aspiration. When transporting the sample, the sample transfer device 5 can remain stationary, transporting the sample solely through tubing. Alternatively, the sample transfer device 5 can be moved to achieve sample transfer.

[0101] Micro-blood samples and macro-blood samples can use the same set of transfer components or different transfer components. Considering the significant difference in blood volume between macro-blood samples and micro-blood samples, one embodiment designs corresponding collection and transfer components for the two different types of samples. The sample transfer device 5 includes a micro-blood sample collection and transfer component and a macro-blood sample collection and transfer component. These components are designed specifically for micro-blood samples and macro-blood samples, respectively. The appropriate collection and transfer components can be designed based on the blood volume of the micro-blood samples and macro-blood samples, enabling better transfer of the corresponding samples.

[0102] When the sample slide preparation device 1 is in the micro-blood mode, the micro-blood sample collection and transfer component picks up the micro-blood sample from the first sample container 91, which has been mixed by the micro-blood sample mixing device 3, and transfers it to the blank slide 100.

[0103] When the sample slide preparation device 1 is in the constant blood mode, the constant blood sample collection and transfer component picks up the constant blood sample mixed by the constant blood sample mixing device 4 in the second sample container 92 and transfers it to the blank slide 100.

[0104] Furthermore, the micro-blood sample collection and transfer assembly includes a first sampling assembly and a first sampling drive assembly for driving the movement of the first sampling assembly. The first sampling assembly may include a first sampling needle 51, a driving gas source for controlling the aspiration of the first sampling needle 51, and other related structures. The movement trajectory of the first sampling assembly has a sample drop position. When the sample slide preparation device 1 operates in micro-blood mode, the first sampling drive assembly drives the first sampling assembly to the sampling position of the sample transport device 2 to aspirate a micro-blood sample from the first sample container 91, which has been mixed by the micro-blood sample mixing device 3, and carries the aspirated micro-blood sample to the sample drop position, transferring the micro-blood sample onto the blank slide 100. The first sampling drive assembly primarily drives the first sampling assembly and can be driven by a motor or other power components. Considering the small amount of blood in a trace blood sample, the same first sampling needle 51 is used for both blood dripping and sampling in this first sampling component. The collected trace blood sample is temporarily stored in the first sampling needle 51 and then discharged from the first sampling needle 51 at the dripping position. This operation can be completed with a small amount of blood and is more suitable for trace blood samples.

[0105] The constant blood sample collection and transfer assembly includes a second sampling component and a second sampling drive component that drives the movement of the second sampling component. This second sampling drive component primarily drives the second sampling component and can be driven by a motor or other power components.

[0106] The second sampling component may include a second sampling needle 52, a driving gas source for controlling the suction of the second sampling needle 52, and other related structures. The driving gas sources for the first sampling needle 51 and the second sampling needle 52 may be the same or different.

[0107] In one embodiment, the second sampling component is connected to the first sampling component via a pipeline. When the sample slide preparation device 1 is in constant blood mode, the second sampling drive component moves the second sampling component to the sampling position of the sample transport device 2 to draw a constant blood sample from the second sample container 92 mixed by the constant blood sample mixing device 4. The sample drawn by the second sampling component is then transferred to the first sampling component via the pipeline. The first sampling drive component then moves the first sampling component to the sample drop position and transfers the sample onto the blank slide 100. This second sampling drive component primarily drives the second sampling component and can be driven by a motor or other power components.

[0108] Since the volume of a normal blood sample is greater than that of a micro-blood sample, in the above embodiment, the second sampling component is connected to the first sampling component via a tubing. Both the micro-blood sample collection and transfer component and the normal blood sample collection and transfer component can be sampled by the first sampling component. Even if some micro-blood sample remains in the tubing, it will not affect the sampling of the micro-blood sample. Furthermore, the second sampling component and the first sampling component both utilize the first sampling needle 51 for sampling, which simplifies the structure and makes the entire device more compact and miniaturized. During sampling control, it is only necessary to move the first sampling needle 51 to the sampling position, making operation simpler.

[0109] In other embodiments, the second sampling component may also perform sampling and dispensing on its own, for example, by setting a separate dispensing needle for dispensing, or by directly using the second sampling needle 52 of the second sampling component for dispensing.

[0110] Furthermore, to simplify the structure, eliminate components, and reduce costs, the first sampling drive component and the second sampling drive component are driven by the same drive module, meaning that a single drive module simultaneously drives both the first and second sampling drive components. When the sample slide preparation device 1 operates in either the constant blood mode or the micro blood mode, this drive module synchronously drives the movement of both the second and first sampling components.

[0111] Please refer to Figure 9 In a more specific example structure, the sample transfer device 5 includes a first sampling needle 51, a second sampling needle 52, motors 53 and 54, a guide rod 55, a slide rail 56, and a sampling needle fixing member 57. The first sampling needle 51 and the second sampling needle 52 are jointly fixed to the sampling needle fixing member 57. Driven by the motor 54, the sampling needle fixing member 57 can move the first sampling needle 51 and the second sampling needle 52 along the slide rail 56 in the Z1 or Z2 direction, for example, to perform operations such as inserting and removing the sample container, or moving closer to and away from the blank slide 100. Driven by the motor 53, the sampling needle fixing member 57 can move the first sampling needle 51 and the second sampling needle 52 along the guide rod 55 in the Y1 or Y2 direction to adjust their positions. The motors 53 and 54 can be stepper motors.

[0112] The sampling position of the sampling device 2 can be located at different positions on the same straight line as the Y-axis as the drop position. Therefore, the first sampling needle 51 and the second sampling needle 52 only need to move along the Y-axis to switch between the two positions. When the movement of the first sampling needle 51 and the second sampling needle 52 along the Y-axis cannot achieve the purpose of switching between the two positions, the switching between the sampling position and the drop position can also be achieved by adding the movement of the first sampling needle 51 and the second sampling needle 52 in the X-axis direction and the superposition of the movement in the Y-axis direction.

[0113] Furthermore, considering the small blood volume characteristic of micro-blood samples, such as Figure 6 and 12 As shown, in one embodiment, a first sampling needle 51 is specifically designed. The first sampling needle 51 has a first channel for sample flow. The bottom end of the first sampling needle 51 is relatively flat and has a downward-opening groove 512. The groove 512 extends at least to one side wall of the first sampling needle 51. The bottom wall of the groove 512 has a first liquid inlet 511. The first liquid inlet 511 communicates with the first channel inside the first sampling needle 51, so that a small amount of blood sample can enter the first channel from the first liquid inlet 511 at the bottom end of the first sampling needle 51.

[0114] The first sampling needle 51 is used to aspirate samples from the first sample container 91. For example... Figure 3 As shown, since the middle area of ​​the cap 911 of the first sample container 91 is made of rubber and has a through cross slit 911a, even if the bottom of the first sampling needle 51 is relatively flat, it can easily squeeze open the cross groove 911a to enter the inner cavity of the first sample container 91 and transfer the sample.

[0115] Furthermore, the sampling hole 511 of the first sampling needle 51 is located at the bottom of the needle. This reduces the minimum sample volume requirement of the first sample container 91 when the first sampling needle 51 is pressed against the bottom of the inner cavity of the first sample container 91 for sampling. It makes it easier to aspirate trace amounts of blood samples, even those with small blood volumes, and avoids situations where samples cannot be collected due to insufficient blood volume. Simultaneously, the radial groove 512 at the bottom of the first sampling needle 51 prevents the sampling hole 511 from being blocked at the bottom of the first sample container 91 when the first sampling needle 51 is pressed against the bottom of the first sample container 91 for sampling.

[0116] Furthermore, considering the relatively abundant blood volume in constant blood samples, such as Figure 6 As shown, in one embodiment, a second sampling needle 52 is specifically designed. The second sampling needle 52 has a second channel, and the head of the second sampling needle 52 is pointed. The side of the head has a second liquid inlet 521 so that the second sample can enter the second channel from the second liquid inlet 521.

[0117] The second sampling needle 52 is used to aspirate samples from the second sample container 92. Since the central area of ​​the cap 921 of the second sample container 92 is made of thick rubber with no through-cut, the bottom of the second sampling needle 52 must be sharp enough to pierce the cap 921 and enter the inner cavity of the second sample container 92 to aspirate samples. Considering that debris may be generated when piercing the rubber and block the aspiration hole, the aspiration hole of the second sampling needle 52 needs to be located on the side of the needle.

[0118] In one embodiment, please refer to Figure 14In addition to aspirating samples, the first sampling needle 51 is also used to drop samples onto the blank glass slide 100. Furthermore, in some embodiments, the first sampling needle 51 and the second sampling needle 52 of the sampling device 5 are connected by a tubing, so the sample aspirated by the second sampling needle 52 can also be dropped onto the blank glass slide 100 via the first sampling needle 51. Therefore, the sampling device 5 also functions as a sample dropping device.

[0119] Furthermore, such as Figure 1 As shown, the slide supply device includes a slide supply device 6 and a first slide transport device 7. The slide supply device 6 is used to supply unused blank slides to the sample slide preparation equipment 1. The slide supply device 6 is equipped with a slide storage section that can store a number of unused blank slides 100. The slide supply device 6 delivers the unused blank slides 100 to the first slide transport device 7 one by one.

[0120] After receiving the blank slide 100, the first slide transport device 7 will perform the following steps:

[0121] Step 1: Horizontally feed the unused blank slide 100 to the slide printing position of the sample slide preparation device 1. At the printing position, the printing device 8 can print information such as the sample number, the name and gender of the sample source, and the department that submitted the sample in the printing area of ​​the blank slide 100.

[0122] Step 2: The first slide transport device 7 horizontally delivers the blank slide 100 to the sample dispensing position of the sample slide preparation device 1. The control unit controls the sampling device 5 to move the first sampling needle 51 along the Y2 direction to directly above the blank slide 100, and then along the Z2 direction so that the bottom of the first sampling needle 51 touches the blank slide 100. Then, all or part of the sample drawn by the first sampling needle 51 is dispensed onto the blank slide 100 (e.g., ...). Figure 14 (As shown). The radial groove 512 at the bottom of the first sampling needle 51 prevents the sampling hole 511 at the bottom of the first sampling needle 51 from being blocked when the first sampling needle 51 is pressed against the blank glass slide 100 drops of blood, thus preventing the sample from dripping out.

[0123] After the first sampling needle 51 of the sampling device 5 drops blood onto the blank slide 100, the control unit controls the sampling device 5 to raise the first sampling needle 51 along the Z1 direction so that the bottom of the first sampling needle 51 is removed from the blank slide 100. Then, the control unit controls the sampling device 5 to move the first sampling needle 51 away from the blank slide 100 along the Y1 direction.

[0124] Then, the control device controls the slide pusher 9 to move the pusher head horizontally above the glass slide 100 with the sample drop. The control device then moves the pusher head along the Z2 direction until it contacts the glass slide 100 with the sample drop. The pusher head then moves laterally relative to the glass slide 100, completing the slide push action and causing the sample drop to become a thin sample film adhering to the glass slide 100. The control device then controls the slide pusher 9 to remove the pusher head from the glass slide 100 after the slide push and cleans the pusher head.

[0125] Step 3: The first slide transport device 7 horizontally feeds the slide 100 after it has been pushed into the flipping device.

[0126] After receiving the slide 100 after it has been pushed, the flipping device begins to flip the slide 100, so that the slide 100 after it has been pushed changes from a flat position to a vertical position.

[0127] Then, the second slide transport device 11 clamps the pushed slide 100 and sends it to the drying position of the sample slide preparation device 1 for drying, and then sends it to the staining device 10. The sample slide preparation device 1 adds staining solution to the staining device 10 to stain the cells. After staining is completed, the second slide transport device 11 clamps the pushed slide 100 and sends it to the cleaning position to clean the residual staining solution on the slide, and then sends the pushed slide 100 into the blank slide storage basket, thus completing the automated slide preparation.

[0128] The above examples illustrate the present invention only to aid in understanding it and are not intended to limit the scope of the invention. Those skilled in the art can make various simple deductions, modifications, or substitutions based on the principles of this invention.

Claims

1. A sample slide making apparatus, comprising: The operation mode of the sample slide making device includes at least a constant blood mode and a micro blood mode, and the sample slide making device comprises: a sample transporting device for transporting sample containers containing samples, wherein the samples transported by the sample transporting device include micro blood samples and constant blood samples, a first sample container is used for storing the micro blood samples, and a second sample container is used for storing the constant blood samples; a micro blood sample mixing device capable of driving the first sample container to move to mix the micro blood samples; a constant blood sample mixing device capable of driving the second sample container to move to mix the constant blood samples; a slide supplying device for supplying blank slides; a sample transferring device for collecting the mixed samples in the first sample container and the second sample container and transferring the collected samples to the blank slides; a slide pushing device for pushing the samples on the blank slides to form sample slides; and a control unit connected in signal with the sample transporting device, the micro blood sample mixing device, the constant blood sample mixing device, the slide supplying device, the sample transferring device and the slide pushing device to control the operation of the sample transporting device, the micro blood sample mixing device, the constant blood sample mixing device, the slide supplying device, the sample transferring device and the slide pushing device, wherein: the control unit determines the operation mode of the sample slide making device according to sample type information; when the operation mode of the sample slide making device is the micro blood mode, the control unit controls the micro blood sample mixing device to mix the micro blood samples in the first sample container, controls the sample transferring device to collect the micro blood samples in the first sample container mixed by the micro blood sample mixing device and transfer the collected micro blood samples to the blank slides, and then controls the slide pushing device to push the micro blood samples on the blank slides to form sample slides; when the operation mode of the sample slide making device is the constant blood mode, the control unit controls the constant blood sample mixing device to mix the constant blood samples in the second sample container, controls the sample transferring device to collect the constant blood samples in the second sample container mixed by the constant blood sample mixing device and transfer the collected constant blood samples to the blank slides, and then controls the slide pushing device to push the constant blood samples on the blank slides to form sample slides.

2. The specimen slide preparation apparatus of claim 1, wherein, The sample slide making device further comprises an identification device connected in signal with the control unit, wherein the identification device is used for obtaining sample type information of the samples transported by the sample transporting device and sending the obtained sample type information to the control unit.

3. The specimen slide preparation apparatus of claim 2, wherein, The identification device determines the sample type information by obtaining at least one of the type of a sample rack in which the sample containers are located, the type of a sample container in which the samples are located and the amount of the samples.

4. The specimen slide preparation apparatus of claim 1, wherein, The sample slide making device further comprises a receiving device, which is in signal connection with the control unit, and is used for receiving an externally input instruction for indicating sample type information and sending the received instruction to the control unit.

5. The specimen slide preparation apparatus of claim 1, wherein, When the operation mode of the sample slide making device is the micro blood mode, the micro blood sample mixing device drives the first sample container to move so as to mix the micro blood sample; during the movement of the first sample container driven by the micro blood sample mixing device, the bottom of the first sample container is kept lower than the container mouth of the first sample container.

6. The specimen slide preparation apparatus of claim 5, wherein, The micro blood sample mixing device comprises a container fixing seat and a first driving assembly, the container fixing seat has a container mounting cavity capable of placing the first sample container, and the first driving assembly is in transmission connection with the container fixing seat. When the operation mode of the sample slide making device is the micro blood mode, the first driving assembly drives the first sample container to alternately rotate forward and backward around the supporting point of the container fixing seat, and during the alternately rotating forward and backward, the bottom of the first sample container is kept lower than the container mouth of the first sample container.

7. The specimen slide preparation apparatus of claim 1, wherein, The sample slide making device further comprises a container transferring device, which is used for transferring the first sample container between the sample conveying device and the micro blood sample mixing device. When the operation mode of the sample slide making device is the micro blood mode, the container transferring device moves the first sample container from the sample conveying device to the micro blood sample mixing device for mixing. After the mixing is completed, the container transferring device moves the first sample container from the micro blood sample mixing device to the sample conveying device.

8. The specimen slide preparation apparatus of claim 1, wherein, When the operation mode of the sample slide making device is the constant blood mode, the constant blood sample mixing device drives the second sample container to move upside down in the vertical direction so as to mix the constant blood sample.

9. The specimen slide preparation apparatus of claim 8, wherein, The constant blood sample mixing device comprises a picking assembly and a constant blood sample mixing driving assembly for driving the picking assembly to move; When the operation mode of the sample slide making device is the constant blood mode, the constant blood sample mixing driving assembly drives the picking assembly to pick up the second sample container from the sample conveying device, and the picking assembly drives the second sample container to move upside down in the vertical direction so as to mix; After the mixing is completed, the constant blood sample mixing driving assembly drives the picking assembly to place the second sample container back to the sample conveying device.

10. The specimen slide preparation apparatus of claim 9, wherein, When the operation mode of the sample slide making device is the micro blood mode, the constant blood sample mixing driving assembly drives the picking assembly to pick up the first sample container from the sample conveying device and move the first sample container to the micro blood sample mixing device for mixing; After the mixing is completed, the constant blood sample mixing driving assembly drives the picking assembly to move the first sample container from the micro blood sample mixing device to the sample conveying device.

11. The specimen slide making apparatus of any one of claims 1-10, wherein, The sample conveying device comprises a micro blood sample collecting and conveying assembly and a constant blood sample collecting and conveying assembly; When the operation mode of the sample slide making device is the micro blood mode, the micro blood sample collecting and transferring assembly sucks the micro blood sample in the first sample container mixed by the micro blood sample mixing device and transfers it to the blank slide; When the operation mode of the sample slide making device is the constant blood mode, the constant blood sample collecting and transferring assembly sucks the constant blood sample in the second sample container mixed by the constant blood sample mixing device and transfers it to the blank slide.

12. The specimen slide preparation apparatus of claim 11, wherein, The micro blood sample collecting and transferring assembly comprises a first sampling assembly and a first sampling driving assembly for driving the first sampling assembly to move, when the operation mode of the sample slide making device is the micro blood mode, the first sampling driving assembly drives the first sampling assembly to move to the sampling position of the sample transporting device to suck the micro blood sample from the first sample container mixed by the micro blood sample mixing device and to carry the sucked micro blood sample to the sample dropping position to transfer the micro blood sample to the blank slide.

13. The specimen slide preparation apparatus of claim 12, wherein, The constant blood sample collecting and transferring assembly comprises a second sampling assembly and a second sampling driving assembly for driving the second sampling assembly to move, the second sampling assembly is in communication with the first sampling assembly through a pipeline; when the operation mode of the sample slide making device is the constant blood mode, the second sampling driving assembly drives the second sampling assembly to move to the sampling position of the sample transporting device to suck the constant blood sample from the second sample container mixed by the constant blood sample mixing device and to make the sample sucked by the second sampling assembly transferred to the first sampling assembly through the pipeline, the first sampling driving assembly drives the first sampling assembly to move to the sample dropping position and to transfer the sample to the blank slide.

14. The specimen slide preparation apparatus of claim 13, wherein, The first sampling driving assembly and the second sampling driving assembly are the same driving module.

15. The specimen slide preparation apparatus of claim 14, wherein, The driving module is used for driving the first sampling assembly and the second sampling assembly, when the operation mode of the sample slide making device is the constant blood mode, the driving module synchronously drives the second sampling assembly and the first sampling assembly to move.

16. A method of making a specimen slide for use in a specimen slide making apparatus, the specimen slide making apparatus comprising: The sample transporting device, the slide supplying device, the identifying device, the sample transferring device, the micro blood sample mixing device, the constant blood sample mixing device and the slide pushing device are characterized in that, The sample transporting device is used for transporting the first sample container containing the micro blood sample and the second sample container containing the constant blood sample; The slide supplying device supplies the blank slide; The identifying device obtains the sample type information of the sample transported by the sample transporting device; When the sample type information obtained by the identifying device indicates that the sample transported by the sample transporting device is the micro blood sample, the first sample container containing the micro blood sample is transferred to the micro blood sample mixing device, the micro blood sample mixing device mixes the sample in the first sample container; the sample transferring device collects the micro blood sample mixed by the micro blood sample mixing device and transfers the collected micro blood sample to the blank slide, and the slide pushing device pushes the micro blood sample on the blank slide to be flat to form a sample slide. The constant blood sample collecting and transferring assembly comprises a second sampling assembly and a second sampling driving assembly for driving the second sampling assembly to move, the second sampling assembly is in communication with the first sampling assembly through a pipeline; when the operation mode of the sample slide making device is the constant blood mode, the second sampling driving assembly drives the second sampling assembly to move to the sampling position of the sample transporting device to suck the constant blood sample from the second sample container mixed by the constant blood sample mixing device and to make the sample sucked by the second sampling assembly transferred to the first sampling assembly through the pipeline, the first sampling driving assembly drives the first sampling assembly to move to the sample dropping position and to transfer the sample to the blank slide. The first sampling driving assembly and the second sampling driving assembly are the same driving module. The driving module is used for driving the first sampling assembly and the second sampling assembly, when the operation mode of the sample slide making device is the constant blood mode, the driving module synchronously drives the second sampling assembly and the first sampling assembly to move. The sample transporting device, the slide supplying device, the identifying device, the sample transferring device, the micro blood sample mixing device, the constant blood sample mixing device and the slide pushing device are characterized in that, The sample transporting device is used for transporting the first sample container containing the micro blood sample and the second sample container containing the constant blood sample; The slide supplying device supplies the blank slide; The identifying device obtains the sample type information of the sample transported by the sample transporting device; When the sample type information obtained by the identifying device indicates that the sample transported by the sample transporting device is the micro blood sample, the first sample container containing the micro blood sample is transferred to the micro blood sample mixing device, the micro blood sample mixing device mixes the sample in the first sample container; the sample transferring device collects the micro blood sample mixed by the micro blood sample mixing device and transfers the collected micro blood sample to the blank slide, and the slide pushing device pushes the micro blood sample on the blank slide to be flat to form a sample slide. When the sample type information obtained by the identification device indicates that the sample carried by the sample carrying device is a constant blood sample, the second sample container containing the constant blood sample is transferred to the constant blood sample mixing device, and the constant blood sample mixing device mixes the constant blood sample in the second sample container; the sample transfer device collects the constant blood sample mixed by the constant blood sample mixing device and transfers the collected constant blood sample to the blank glass slide, and the pusher device pushes the constant blood sample on the blank glass slide to form a sample slide.

17. The method of claim 16, wherein: The micro blood sample mixing device mixes the micro blood sample in the first sample container, and specifically includes the following steps: The micro blood sample mixing device drives the first sample container to move to mix the micro blood sample, and the bottom of the first sample container is kept lower than the container port of the first sample container during the movement of the first sample container.

18. The method of claim 16, wherein: The micro blood sample mixing device includes a container fixing seat and a first driving assembly, the container fixing seat has a container mounting cavity capable of placing the first sample container, and the first driving assembly is in transmission connection with the container fixing seat. When the operation mode of the sample slide making equipment is the micro blood mode, the first driving assembly drives the first sample container to alternately rotate forward and reverse around the supporting point of the container fixing seat, and the bottom of the first sample container is kept lower than the container port of the first sample container during the alternately rotating forward and reverse.

19. The method of claim 16, wherein: Further comprising: The first sample container containing the micro blood sample is transferred to the micro blood sample mixing device, and the micro blood sample mixing device mixes the micro blood sample in the first sample container, and further comprising: The container transfer device moves the first sample container from the micro blood sample mixing device to the sample carrying device.

20. The sample slide preparation method as described in claim 16, characterized in that, The sample transfer device includes a first sampling assembly and a first sampling driving assembly for driving the first sampling assembly to move, when the sample type information obtained by the identification device points to the micro blood sample, the first sampling driving assembly drives the first sampling assembly to move to the sampling position of the sample carrying device to suck the micro blood sample from the mixed first sample container, and carries the sucked micro blood sample to the sample dropping position to transfer the micro blood sample to the blank glass slide.

21. The method for preparing a sample slide as described in claim 20, characterized in that, The sample transfer device further includes a second sampling assembly and a second sampling driving assembly for driving the second sampling assembly to move, and the second sampling assembly is in communication with the first sampling assembly through a pipeline; when the sample type information obtained by the identification device points to the constant blood sample, the second sampling driving assembly drives the second sampling assembly to move to the sampling position of the sample carrying device to suck the sample from the mixed second sample container, and the sample sucked by the second sampling assembly is transported to the first sampling assembly, and then the first sampling assembly is controlled to move to the sample dropping position and transfer the sample to the blank glass slide.

22. The method of claim 21, wherein the step of applying the adhesive to the sample slide comprises applying the adhesive to the sample slide in a pattern that is substantially free of adhesive in the center of the sample slide. The first sampling driving assembly and the second sampling driving assembly are the same driving module.

23. The sample slide preparation method as described in claim 22, characterized in that, The driving module is used for driving the first sampling component and the second sampling component, and when the operation mode of the sample slide manufacturing equipment is the constant blood mode, the driving module synchronously drives the second sampling component and the first sampling component to move.

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