Sampler and sampling method

Abstract

The present invention belongs to the field of medical device technology, and in particular, to a sampler and a sampling method. In the sampler, the first external liquid extractor is used to extract the target solution in the external cell container into the accommodating cavity through the first joint and the first interface in sequence; the first external liquid extractor is also used to push the target solution remaining in the second joint, the first joint, and the pipeline between the first joint and the second joint back into the external cell container after the pipelines between the first interface and the accommodating cavity and between the second interface and the accommodating cavity are closed. In the present invention, the waste of the target solution caused by the target solution remaining in the second joint, the first joint, the pipeline between the first joint and the first interface, and the pipeline between the second joint and the second interface is avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of medical devices, and in particular relates to a sampler and a sampling method. Background Art

[0002] With the continuous advancement of medical technology, the technology of using cell solutions to treat patients has been continuously applied by major hospitals. After the cell solution is cultured in the cell container, it needs to be transferred to other containers for processing, such as transferring to a centrifuge tube for centrifugal treatment, moving to a cryopreservation tube for frozen storage, etc. In addition, during the production process of cell-based drugs, it is necessary to regularly sample the cell solution in the cell container to monitor indicators such as cell activity, loss rate, and density. In order to ensure that the external environment does not interfere with the cell solution, sampling of the cell solution needs to be carried out in a sterile environment.

[0003] In the prior art, after sampling the cell solution, the sampling port needs to be sealed, so that the cell solution will remain in the sampling port and the pipeline near the sampling port, and the cell solution cannot be used, resulting in a waste of the cell solution. The cell solution extracted from the blood of critically ill patients is very scarce, so every drop of cell solution is precious. Therefore, the existing sampler will cause a waste of cell solution when sampling the cell solution, which does not meet the requirements of modern medicine for cell solution. Summary of the invention

[0004] The present invention aims at the technical problem that waste is caused when a sampler in the prior art samples a cell solution, and provides a sampler and a sampling method.

[0005] In view of the above technical problems, an embodiment of the present invention provides a sampler, comprising a first joint, a second joint and a sampling container, wherein the sampling container is provided with a receiving cavity, a first interface and a second interface; one end of the first joint is connected to an external cell container, and the other end of the first joint is connected to the receiving cavity and the second interface respectively through the first interface; one end of the second joint is connected to a first external liquid extractor, and the other end of the second joint is connected to the receiving cavity and the first interface respectively through the second interface;

[0006] The first external liquid extractor is used to extract the target solution in the external cell container into the containing cavity through the first joint and the first interface in sequence;

[0007] The first external liquid pump is also used to push the target solution remaining in the second joint, the first joint, and the pipeline between the first joint and the second joint back into the external cell container after the pipelines between the first interface and the accommodating chamber and between the second interface and the accommodating chamber are closed.

[0008] Optionally, the sampling container includes a three-way tube and a receiving tube, the first interface and the second interface are both arranged on the three-way tube, the three-way tube is further provided with a third interface connected to both the first interface and the second interface, the receiving chamber is arranged on the receiving tube; the receiving chamber is connected to the first interface and the second interface through the third interface;

[0009] After the pipeline between the third interface and the accommodating tube is closed, the pipelines between the first interface and the accommodating chamber and between the second interface and the accommodating chamber are all closed.

[0010] Optionally, the sampling container further comprises a first tube clamp, and the first tube clamp is used to clamp a tube between the third interface and the containing tube;

[0011] After controlling the first tube clamp to clamp the pipeline between the third interface and the containing tube and performing a heat-sealing process on the pipeline, the pipeline between the third interface and the containing tube is closed.

[0012] Optionally, the sampling container further comprises a third joint, one end of the third joint is connected to an end of the containing tube away from the third interface, and the other end of the third joint is connected to a second external liquid extractor;

[0013] The second external liquid extractor is used to extract the target solution in the containing chamber after the end of the containing tube away from the third joint is connected.

[0014] Optionally, the sampling container includes a U-shaped flexible tube and a connecting tube, the accommodating chamber is a U-shaped cavity arranged on the U-shaped flexible tube, the first interface and the second interface are respectively arranged at opposite ends of the U-shaped cavity, one end of the connecting tube is connected to the first joint through the first interface, and the other end of the connecting tube is connected to the second joint through the second interface.

[0015] Optionally, the sampling container further comprises a second tube clamp and a third tube clamp, wherein the second tube clamp is used to clamp the pipeline between the first interface and the accommodating cavity, and the third tube clamp is used to clamp the pipeline between the second interface and the accommodating cavity;

[0016] After controlling the second pipe clamp to clamp the pipe between the first interface and the accommodating cavity and heat-sealing the pipe, the pipe between the first interface and the accommodating cavity is sealed;

[0017] After the third pipe clamp is controlled to clamp the pipe between the second interface and the accommodating cavity and heat-seals it, the pipe between the second interface and the accommodating cavity is closed.

[0018] Optionally, the sampling container is provided with scale lines for marking the volume of the target solution in the containing cavity.

[0019] Optionally, the sampler further comprises a first protective cap and a second protective cap, wherein the first protective cap is detachably covered on an end of the first joint away from the sampling container, and the second protective cap is detachably covered on an end of the second joint away from the sampling container.

[0020] Optionally, the sampler further comprises a filter, wherein the filter is installed at an end of the second joint away from the sampling container, and the first external liquid extractor is connected to the second joint via the filter; the filter is used for filtering the gas entering the sampling container.

[0021] In the present invention, the first external liquid extractor is used to extract the target solution in the external cell container into the accommodating chamber through the first joint and the first interface in sequence; the first external liquid extractor is also used to push the target solution remaining in the second joint, the first joint, and the pipeline between the first joint and the second joint back to the external cell container after the pipelines between the first interface and the accommodating chamber and between the second interface and the accommodating chamber are closed; thereby avoiding the waste of the target solution caused by the target solution remaining in the second joint, the first joint, the pipeline between the first joint and the first interface, and the pipeline between the second joint and the second interface; the sampler of the present invention can realize simple and fast sampling of the target solution, and can effectively ensure the aseptic sampling of the target solution, greatly reducing the probability of the target solution being contaminated; and the sampling of the sampler of the present invention is accurate, and can realize multiple, quantitative, aseptic, and disposable high-precision sampling of the target solution; at the same time, it avoids waste, reduces the connection requirements of the operator, and achieves the effect of compatible connection. In addition, the sampler has a simple structure, low manufacturing cost, and convenient operation.

[0022] Another embodiment of the present invention further provides a sampling method of the above sampler, comprising:

[0023] receiving a sampling instruction, and controlling the first external liquid extractor to extract the target solution in the external cell container into the containing cavity through the first joint and the first interface in sequence;

[0024] Controlling the closure of the pipelines between the first interface and the accommodating chamber and between the second interface and the accommodating chamber;

[0025] Controlling the first external liquid extractor to push the target solution remaining in the second joint, the first joint, and the pipeline between the first joint and the second joint back into the external cell container;

[0026] The sampling container is disassembled and separated from the first joint and the second joint, and the target solution in the containing cavity is taken out.

[0027] Optionally, the sampling container includes a three-way tube and a receiving tube, the first interface and the second interface are both arranged on the three-way tube, the three-way tube is also provided with a third interface connected to both the first interface and the second interface, the receiving cavity is arranged on the receiving tube; the receiving cavity is connected to the first interface and the second interface through the third interface; the sampling container also includes a first tube clamp, the first tube clamp is used to clamp the pipeline between the third interface and the receiving tube;

[0028] The controlling the closure of the pipelines between the first interface and the accommodating chamber and between the second interface and the accommodating chamber comprises:

[0029] When the end of the containing tube away from the third joint is closed, the first tube clamp is controlled to clamp the pipeline between the third interface and the containing tube and perform heat sealing treatment on it, and then it is confirmed that the pipeline between the first interface and the containing cavity and between the second interface and the containing cavity are closed.

[0030] Optionally, the sampling container further comprises a third joint, one end of the third joint is connected to an end of the containing tube away from the third interface, and the other end of the third joint is connected to a second external liquid extractor;

[0031] The step of disassembling and separating the sampling container from the first joint and the second joint, and taking out the target solution in the containing cavity, comprises:

[0032] Controlling the end of the accommodating tube clamped by the first tube clamp to be disassembled and separated from the third interface;

[0033] After the end of the containing tube away from the third joint is connected, the second external liquid extractor is controlled to extract the target solution in the containing chamber.

[0034] Optionally, the sampling container includes a U-shaped flexible tube and a connecting tube, the accommodating cavity is a U-shaped cavity provided on the U-shaped flexible tube, the first interface and the second interface are respectively provided at opposite ends of the U-shaped cavity, one end of the connecting tube is connected to the first joint through the first interface, and the other end of the connecting tube is connected to the second joint through the second interface;

[0035] The step of disassembling and separating the sampling container from the first joint and the second joint, and taking out the target solution in the containing cavity, comprises:

[0036] Controlling the closed opposite ends of the U-shaped flexible tube to be disassembled and separated from the first interface and the second interface respectively;

[0037] After connecting both ends or one end of the U-shaped flexible tube, the target solution in the U-shaped cavity is controlled to be squeezed out.

[0038] Optionally, the sampling container further comprises a second tube clamp and a third tube clamp, wherein the second tube clamp is used to clamp the pipeline between the first interface and the accommodating cavity, and the third tube clamp is used to clamp the pipeline between the second interface and the accommodating cavity;

[0039] The controlling the closure of the pipelines between the first interface and the accommodating chamber and between the second interface and the accommodating chamber comprises:

[0040] After controlling the second tube clamp to clamp the pipeline between the first interface and the accommodating cavity and perform heat sealing treatment on it, and controlling the third tube clamp to clamp the pipeline between the second interface and the accommodating cavity and perform heat sealing treatment on it, it is confirmed that the pipelines between the first interface and the accommodating cavity and between the second interface and the accommodating cavity are closed.

[0041] The sampling method of the present invention first controls the closure of the pipelines between the first interface and the accommodating cavity and between the second interface and the accommodating cavity; then controls the first external liquid extractor to push the target solution remaining in the pipeline between the second joint, the first joint, and the first joint and the second joint back into the external cell container; finally, the sampling container can be disassembled and separated from the first joint and the second joint, and the target solution in the accommodating cavity is taken out, and the sampling of the target solution is completed. The sampling method of the present invention can realize simple and fast sampling of the target solution, and can effectively ensure the aseptic sampling of the target solution, greatly reducing the probability of the target solution being contaminated; and the sampling method of the present invention is accurate in sampling, and can realize multiple, quantitative, aseptic, and disposable high-precision sampling of the target solution; at the same time, it avoids waste, reduces the connection requirements of the operator, and achieves the effect of compatible connection. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0043] Figure 1 is a schematic structural diagram of a sampler provided by an embodiment of the present invention;

[0044] Figure 2 is a schematic structural diagram of a sampler provided by another embodiment of the present invention;

[0045] Figure 3 It is a schematic diagram of a sampling method provided by an embodiment of the present invention.

[0046] The reference numerals in the specification are as follows:

[0047] 1. First joint; 2. Second joint; 3. Sampling container; 31. Accommodating chamber; 32. First interface; 33. Second interface; 34. Three-way pipe; 341. Third interface; 35. Accommodating tube; 36. First tube clamp; 37. Third joint; 301. U-shaped flexible tube; 302. Connecting tube; 303. Second tube clamp; 304. Third tube clamp; 4. First protective cap; 5. Second protective cap; 6. Filter. DETAILED DESCRIPTION

[0048] In order to make the technical problems, technical solutions and beneficial effects solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0049] It should be understood that the directions or positional relationships indicated by terms such as "upper", "lower", "left", "right", "front", "back", and "middle" are based on the directions or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation of the present invention.

[0050] like Figure 1 and Figure 2 As shown, a sampler provided by one embodiment of the present invention includes a first connector 1, a second connector 2 and a sampling container 3, wherein the sampling container 3 is provided with a accommodating cavity 31, a first interface 32 and a second interface 33; one end of the first connector 1 is connected to an external cell container, and the other end of the first connector 1 is connected to the accommodating cavity 31 and the second interface 33 respectively through the first interface 32; one end of the second connector 2 is connected to a first external liquid extractor, and the other end of the second connector 2 is connected to the accommodating cavity 31 and the first interface 32 respectively through the second interface 33; it can be understood that a pipeline is provided between the first interface 32 and the first connector 1, and a pipeline is also provided between the second interface 33 and the second connector 2; the target solution (cell solution) is stored in the external cell container, and the first external liquid extractor includes but is not limited to a syringe, etc.

[0051] The first external liquid extractor is used to extract the target solution in the external cell container into the containing chamber 31 in sequence through the first connector 1 and the first interface 32; it can be understood that after the first connector 1 is connected to the interface of the external cell container and the first external liquid extractor is connected to the second connector 2, the first external liquid extractor is used to extract the sampler into a vacuum state, and the sampler in the vacuum state can extract the cell fluid in the external cell container into the containing chamber 31 through the first connector 1.

[0052] The first external liquid extractor is also used to push the target solution remaining in the second joint 2, the first joint 1, and the pipeline between the first joint 1 and the second joint 2 back to the external cell container after the pipelines between the first interface 32 and the accommodating chamber 31 and between the second interface 33 and the accommodating chamber 31 are closed. It can be understood that the pipeline between the first interface 32 and the accommodating chamber 31 is closed by heat sealing or the like, and the pipeline between the second interface 33 and the accommodating chamber 31 is closed. At this time, the accommodating chamber 31 is closed (that is, the accommodating chamber 31 is in a closed state); the first external liquid extractor is used to fill the sampler with gas, and the gas can reflow the cell fluid remaining in the second joint 2, the first joint 1, the pipeline between the first joint 1 and the first interface 32, and the pipeline between the second joint 2 and the second interface 33 into the external cell container.

[0053] Furthermore, the pipeline between the first interface 32 and the accommodating chamber 31 is cut, and the pipeline between the second interface 33 and the accommodating chamber 31 is cut, at this time, the opposite ends of the accommodating chamber 31 are in a closed state; the sampling container 3 storing the target solution and in a closed state is taken away; then an opening is opened on the sampling container 3, and the target solution in the sampling container 3 is transported to an external container by squeezing, injection, etc., thereby completing the sampling of the target solution.

[0054] In the present invention, the first external liquid extractor is used to extract the target solution in the external cell container into the accommodating chamber 31 through the first joint 1 and the first interface 32 in sequence; the first external liquid extractor is also used to push the target solution remaining in the second joint 2, the first joint 1, and the pipeline between the first joint 1 and the second joint 2 back into the external cell container after the pipelines between the first interface 32 and the accommodating chamber 31 and between the second interface 33 and the accommodating chamber 31 are closed; thereby avoiding the waste of the target solution caused by the target solution remaining in the second joint 2, the first joint 1, the pipeline between the first joint 1 and the first interface 32, and the pipeline between the second joint 2 and the second interface 33; the sampler of the present invention can realize simple and fast sampling of the target solution, and can effectively ensure the aseptic sampling of the target solution, greatly reducing the probability of the target solution being contaminated; and the sampling of the sampler of the present invention is accurate, and can realize multiple, quantitative, aseptic, and disposable high-precision sampling of the target solution; at the same time, it avoids waste, reduces the connection requirements of the operator, and achieves the effect of compatible connection. In addition, the sampler has a simple structure, low manufacturing cost and convenient operation.

[0055] In one embodiment, if Figure 1 As shown, the sampling container 3 includes a three-way pipe 34 and a receiving tube 35, the first interface 32 and the second interface 33 are both arranged on the three-way pipe 34, and the three-way pipe 34 is also provided with a third interface 341 connected to the first interface 32 and the second interface 33, and the receiving chamber 31 is arranged on the receiving tube 35; the receiving chamber 31 is connected to the first interface 32 and the second interface 33 through the third interface 341; it can be understood that a pipeline is provided between the third interface 341 and the receiving tube 35, and the receiving tube 35 includes but is not limited to a drip bucket, a hose, etc.; In the process of using the first external liquid pump to extract the target solution in the external cell container into the containing chamber 31, the containing tube 35 is located below the first connector 1 and the second connector 2. Therefore, in the process of the first external liquid pump extracting the target solution, the target solution will flow into the containing space of the containing tube 35 through the third interface 341 due to its own gravity, and will not flow into the second connector 2 through the second interface 33, further avoiding the waste of the target solution caused by the target solution entering the second connector 2 and the pipeline between the second interface 33 and the second connector 2.

[0056] After the pipeline between the third interface 341 and the receiving tube 35 is closed, the pipelines between the first interface 32 and the receiving chamber 31 and between the second interface 33 and the receiving chamber 31 are all closed. It can be understood that the receiving chamber 31 is connected to the first interface 32 and the second interface 33 through the third interface 341, so that by closing the pipeline between the third interface 341 and the receiving tube 35, the end of the receiving tube 35 facing the third interface 341 can be closed; illustratively, the end of the receiving tube 35 away from the third interface 341 is in a closed state, so that the receiving tube 35 storing the target solution and in a closed state can be sampled; finally, the pipeline connected to the receiving tube 35 (that is, the pipeline between the third interface 341 and the receiving tube 35) is connected, and then the target solution stored in the receiving tube 35 is input into an external vessel (test tube, centrifuge cup, culture dish) by squeezing and gas injection, thereby completing the sampling of the target solution. In this embodiment, the operational convenience of the sampler is further improved.

[0057] It should be noted that the sampling container may include a plurality of three-way tubes 34 and a plurality of the containing tubes 35 connected in series, and two adjacent three-way tubes 34 are connected via a second interface 33 and the first interface 32, and the third interface 341 of each three-way tube is connected to the containing tube 35; when the external pump extracts the target solution, the target solution will flow into the containing tubes 35 of different branches in turn, and when the target number of containing tubes 35 are all filled with the target solution, the external pump stops pumping; by removing the closed containing tubes 35 from different three-way tubes 34 respectively, the sampler can perform the sampling work of the plurality of containing tubes 35 in turn, which improves the sampling efficiency and avoids the target solution flowing through the three-way tube 34 flowing toward the direction of the filter 4 when the flow rate of the target solution is too large during the pumping process of the external pump.

[0058] In one embodiment, if Figure 1 As shown, the sampling container 3 also includes a first tube clamp 36, and the first tube clamp 36 is used to clamp the pipeline between the third interface 341 and the accommodating tube 35; it can be understood that when the first tube clamp 36 clamps the pipeline between the third interface 341 and the accommodating tube 35, the pipeline between the third interface 341 and the accommodating tube 35 is in a closed state, and the pipeline closed by the first tube clamp 36 cannot maintain its closed state for too long.

[0059] After the first tube clamp 36 is controlled to clamp the pipeline between the third interface 341 and the receiving tube 35 and perform heat sealing treatment on it, the pipeline between the third interface 341 and the receiving tube 35 is closed. It can be understood that after the first tube clamp 36 clamps and closes the pipeline between the third interface 341 and the receiving tube 35, the pipeline between the first tube clamp 36 and the third interface 341 is heat sealed by a heat sealing machine. At this time, a heat sealing section is formed on the pipeline between the first tube clamp 36 and the third interface 341, and it is disconnected from the heat sealing section (can be cut with scissors), the third interface 341 is in a closed state, and the receiving tube 35 is also in a closed state. When sampling the target solution in the receiving tube 35, first cut and conduct the pipeline clamped by the first tube clamp 36, then open the first tube clamp 36, and finally input the target solution in the receiving tube 35 into the external container. In this embodiment, the design of the first tube clamp 36 avoids the accident of leakage of the target solution during the heat-sealing operation and sampling of the sampler.

[0060] In one embodiment, if Figure 1 As shown, the sampling container 3 also includes a third joint 37, one end of the third joint 37 is connected to one end of the accommodating tube 35 away from the third interface 341, and the other end of the third joint 37 is connected to a second external liquid extractor; it can be understood that the second external liquid extractor includes but is not limited to a syringe, etc., and a pipeline can be arranged between the accommodating tube 35 and the third interface 341.

[0061] The second external liquid extractor is used to extract the target solution in the accommodating chamber 31 after the end of the accommodating tube 35 away from the third joint 37 is connected. It can be understood that the end of the accommodating tube 35 away from the second external syringe is connected to the external container, and the second external syringe is used to inject gas into the accommodating tube 35 so that the target solution stored in the accommodating tube 35 is input into the external container, thereby completing the sampling of the target solution. In this embodiment, the second external liquid extractor injects the target solution in the accommodating tube 35 into the external container through the third joint 37, thereby further improving the convenience of the sampler.

[0062] In one embodiment, if Figure 2As shown, the sampling container 3 includes a U-shaped flexible tube 301 and a connecting tube 302. The accommodating chamber 31 is a U-shaped cavity arranged on the U-shaped flexible tube 301. The first interface 32 and the second interface 33 are respectively arranged at opposite ends of the U-shaped cavity. One end of the connecting tube 302 is connected to the first joint 1 through the first interface 32, and the other end of the connecting tube 302 is connected to the second joint 2 through the second interface 33. It can be understood that the connecting tube 302 spans between the first interface 32 and the second interface 33, and the U-shaped cavity is located below the first interface 32, the second interface 33 and the connecting tube 302. The shape of the U-shaped cavity can be set according to demand, such as a drip bucket shape, or a shape of continuous quantitative spheres in series, etc., which is not limited here. The first interface 32 and the second interface 33 are located at opposite ends of the connecting tube 302. In this embodiment, the sampling container 3 has a simple structure and low manufacturing cost.

[0063] In one embodiment, if Figure 2 As shown, the sampling container 3 also includes a second tube clamp 303 and a third tube clamp 304, the second tube clamp 303 is used to clamp the pipeline between the first interface 32 and the U-shaped cavity, and the third tube clamp 304 is used to clamp the pipeline between the second interface 33 and the U-shaped cavity; it can be understood that when the second tube clamp 303 clamps the U-shaped flexible tube 301 between the first interface 32 and the accommodating cavity 31, and the third tube clamp 304 clamps the U-shaped flexible tube 301 between the second interface 33 and the accommodating cavity 31, the opposite ends of the U-shaped flexible tube 301 are in a closed state, and the closed state of the U-shaped flexible tube 301 clamped by the second tube clamp 303 and the third tube clamp 304 cannot be maintained for too long.

[0064] After controlling the second tube clamp 303 to clamp the pipeline between the first interface 32 and the accommodating cavity 31 and performing heat sealing treatment on it, the pipeline between the first interface 32 and the accommodating cavity 31 is closed; it can be understood that firstly, after using the second tube clamp 303 to clamp the pipeline of the U-shaped flexible tube 301 between the first interface 32 and the accommodating cavity 31, a heat sealing machine is used to heat seal the pipeline between the second tube clamp 303 and the first interface 32, thereby completing the sealing work of the pipeline between the first interface 32 and the accommodating cavity 31.

[0065] After the third tube clamp 304 is controlled to clamp the pipeline between the second interface 33 and the accommodating cavity 31 and heat-seal the pipeline, the pipeline between the second interface 33 and the accommodating cavity 31 is sealed. It can be understood that after the third tube clamp 304 is first used to clamp the pipeline of the U-shaped flexible tube 301 between the second interface 33 and the accommodating cavity 31, the heat-sealing machine is used to heat-seal the pipeline between the third tube clamp 304 and the second interface 33, thereby completing the sealing work of the pipeline between the second interface 33 and the accommodating cavity 31.

[0066] In one embodiment, if Figure 1 As shown, the sampling container 3 is provided with scale lines for marking the volume of the target solution in the containing cavity 31. It can be understood that the scale can be engraved on the outer wall of the sampling container 3, and the user can clearly know the volume of the target solution entering the containing cavity 31 through the scale lines, so as to avoid the accident of extracting too much target solution and causing waste of target solution, and avoid the accident of extracting too little target solution and causing insufficient sample volume.

[0067] In one embodiment, if Figure 1 and Figure 2 As shown, the sampler further includes a first protective cap 4 and a second protective cap 5, wherein the first protective cap 4 is detachably covered on one end of the first joint 1 away from the sampling container 3, and the second protective cap 5 is detachably covered on one end of the second joint 2 away from the sampling container 3. It can be understood that the first joint 1 includes but is not limited to a Luer joint, and the first protective cap 4 includes but is not limited to a Luer protective cap, etc.; the second joint 2 includes but is not limited to a female Luer joint, etc.; and the first protective cap 4 covers the first joint 1, and the second protective cap 5 covers the second joint 2, thereby ensuring the closedness of the sampler; when the cell solution sampler needs to be used to sample the cell solution, the first protective cap 4 and the second protective cap 5 are removed, and the first joint 1 is connected to an external cell container, and the second joint 2 is connected to a first external liquid extractor.

[0068] In a specific embodiment, the first joint 1 is provided with a first connection thread (not shown in the figure), the first protective cap 4 is provided with a second connection thread (not shown in the figure), and the first protective cap 4 is covered on the first joint 1 through the first connection thread and the second connection thread connected by threads. It can be understood that the first connection thread can be a first external thread provided on the first joint 1, and correspondingly, the second connection thread is a first internal thread hole provided on the first protective cap 4; or, the first connection thread can be a first internal thread hole provided on the first joint 1, and correspondingly, the second connection thread is a first external thread provided on the first protective cap 4. The first external thread is connected to the first internal thread hole, so that the first protective cap 4 is covered on the first joint 1. Further, the interface between the first joint 1 and the external cell container is also connected by threads. In this embodiment, the disassembly and assembly between the first protective cap 4 and the first joint 1 is simple.

[0069] The second joint 2 is provided with a third connection thread (not shown in the figure), and the second protective cap 5 is provided with a fourth connection thread (not shown in the figure), and the second protective cap 5 is covered on the second joint 2 by the third connection thread and the fourth connection thread connected by threads. It can be understood that the third connection thread can be a second external thread provided on the second joint 2, and correspondingly, the fourth connection thread is a second internal thread hole provided on the second protective cap 5; or the third connection thread can be a second internal thread hole provided on the second joint 2, and correspondingly, the fourth connection thread is a second external thread provided on the second protective cap 5. The second external thread is connected to the second internal thread hole, so that the second protective cap 5 is covered on the second joint 2. Furthermore, the second joint 2 and the interface of the first external liquid extractor are also connected by threads. In this embodiment, the disassembly and assembly between the second protective cap 5 and the second joint 2 are simple.

[0070] In one embodiment, if Figure 1 and Figure 2As shown, the sampler further includes a filter 6, which is installed at one end of the second joint 2 away from the sampling container 3, and the first external liquid extractor is connected to the second joint 2 through the filter 6; the filter 6 is used to filter the gas entering the sampling container 3. It can be understood that the filter 6 can be sealed and connected to the end of the second joint 2 away from the sampling container 3 through a dispensing process, and the first external liquid extractor is connected to the end of the filter 6 away from the second joint 2, so that when the first external liquid extractor injects gas into the sampling container 3 through the filter 6 and the second joint 2 in turn, the filter 6 prevents impurities in the gas from entering the sampling container 3, thereby preventing the gas from contaminating the target solution and ensuring the cleanliness of the target solution.

[0071] like Figure 3 As shown, another embodiment of the present invention further provides a sampling method of the above-mentioned sampler, comprising:

[0072] S100, receiving a sampling instruction, and controlling the first external liquid extractor to extract the target solution in the external cell container into the containing chamber 31 through the first connector 1 and the first interface 32 in sequence; it can be understood that the sampling instruction can be an instruction generated after the user triggers the corresponding button. Specifically, after the first connector 1 is connected to the interface of the external cell container and the first external liquid extractor is connected to the second connector 2, the first external liquid extractor is used to extract the sampler into a vacuum state, and the sampler in the vacuum state can extract the cell fluid in the external cell container into the containing chamber 31 through the first connector 1.

[0073] S200, controlling the closure of the pipelines between the first interface 32 and the accommodating chamber 31 and between the second interface 33 and the accommodating chamber 31; it can be understood that the pipelines between the first interface 32 and the accommodating chamber 31 are closed by heat sealing or the like, and the pipelines between the second interface 33 and the accommodating chamber 31 are closed, at which time the accommodating chamber 31 is closed (that is, the accommodating chamber 31 is in a closed state).

[0074] S300, control the first external liquid pump to push the target solution remaining in the second connector 2, the first connector 1, and the pipeline between the first connector 1 and the second connector 2 back into the external cell container; it can be understood that by using the first external liquid pump to fill the sampler with gas, the gas can make the cell fluid remaining in the second connector 2, the first connector 1, the pipeline between the first connector 1 and the first interface 32, and the pipeline between the second connector 2 and the second interface 33 flow back into the external cell container.

[0075] S400, disassemble and separate the sampling container 3 from the first connector 1 and the second connector 2, and take out the target solution in the accommodating chamber 31. It can be understood that the pipeline between the first interface 32 and the accommodating chamber 31 is cut off, and the pipeline between the second interface 33 and the accommodating chamber 31 is cut off, at this time, the opposite ends of the accommodating chamber 31 are in a closed state; take out the sampling container 3 that stores the target solution and is in a closed state; then open an opening on the sampling container 3, and transfer the target solution in the sampling container 3 to an external container by squeezing, injection, etc., thereby completing the sampling of the target solution.

[0076] The sampling method of the present invention can realize simple and quick sampling of the target solution, and can effectively ensure the aseptic sampling of the target solution, greatly reducing the probability of the target solution being contaminated; and the sampling method of the present invention is accurate in sampling, and can realize multiple, quantitative, aseptic, and disposable high-precision sampling of the target solution; at the same time, it avoids waste, reduces the connection requirements of the operator, and achieves the effect of compatible connection.

[0077] In one embodiment, if Figure 1 As shown, the sampling container 3 includes a three-way pipe 34 and a accommodating tube 35, the first interface 32 and the second interface 33 are both arranged on the three-way pipe 34, and the three-way pipe 34 is also provided with a third interface 341 connected to both the first interface 32 and the second interface 33, and the accommodating chamber 31 is arranged on the accommodating tube 35; the accommodating chamber 31 is connected to the first interface 32 and the second interface 33 through the third interface 341; the sampling container 3 also includes a first tube clamp 36, and the first tube clamp 36 is used to clamp the pipeline between the third interface 341 and the accommodating tube 35; it can be understood that a pipeline is provided between the third interface 341 and the accommodating tube 35, and the accommodating tube 35 includes but is not limited to a drip bucket, a hose, etc.; and when the first tube clamp 36 clamps the pipeline between the third interface 341 and the accommodating tube 35, the pipeline between the third interface 341 and the accommodating tube 35 is in a closed state, and the pipeline closed by the first tube clamp 36 cannot be maintained for too long.

[0078] The controlling of closing the pipelines between the first interface 32 and the accommodating chamber 31 and between the second interface 33 and the accommodating chamber 31 (ie, step S200 ) includes:

[0079] When the end of the receiving tube 35 away from the third joint 37 is closed, the first tube clamp 36 is controlled to clamp the pipeline between the third interface 341 and the receiving tube 35 and perform heat sealing treatment on it, and then it is confirmed that the pipeline between the first interface 32 and the receiving cavity 31 and between the second interface 33 and the receiving cavity 31 are closed. It can be understood that the end of the receiving tube 35 away from the third joint 37 can be closed by a second external liquid extractor, and after the first tube clamp 36 clamps and closes the pipeline between the third interface 341 and the receiving tube 35, the pipeline between the first tube clamp 36 and the third interface 341 is heat sealed by a heat sealing machine. At this time, a heat sealing section is formed on the pipeline between the first tube clamp 36 and the third interface 341, and it is disconnected from the heat sealing section (can be cut by scissors), the third interface 341 is in a closed state, and the receiving tube 35 is also in a closed state.

[0080] In one embodiment, if Figure 1 As shown, the sampling container 3 also includes a third joint 37, one end of the third joint 37 is connected to one end of the accommodating tube 35 away from the third interface 341, and the other end of the third joint 37 is connected to a second external liquid extractor; it can be understood that the second external liquid extractor includes but is not limited to a syringe, etc., and a pipeline can be arranged between the accommodating tube 35 and the third interface 341.

[0081] The step of disassembling and separating the sampling container 3 from the first connector 1 and the second connector 2 and taking out the target solution in the containing cavity 31 (ie, step S400 ) includes:

[0082] The end of the containing tube 35 clamped by the first tube clamp 36 is controlled to be disassembled and separated from the third interface 341; it can be understood that the containing tube 35 is separated from the heat-sealed sealing section of the heat-sealing treatment, and the separated containing tube 35 is in a closed state, and the first interface 32 and the second interface 33 are not connected to the external environment, so that the first external liquid extractor can be filled with gas through the pipeline between the second connector 2 and the first interface 32.

[0083] After the end of the receiving tube 35 away from the third joint 37 is connected, the second external liquid extractor is controlled to extract the target solution in the receiving chamber 31. It can be understood that the heat-sealed section of the pipeline connected to the receiving tube 35 can be cut off with scissors, and the pipeline is connected to the external container, and then the first tube clamp 36 is opened. At this time, the end of the receiving tube 35 away from the third joint 37 is in a conducting state; finally, the second external liquid extractor is used to transport the target solution in the receiving tube 35 to the external container, thereby completing the sampling of the target solution.

[0084] In one embodiment, if Figure 2 As shown, the sampling container 3 includes a U-shaped flexible tube 301 and a connecting tube 302, the accommodating cavity 31 is a U-shaped cavity arranged on the U-shaped flexible tube 301, the first interface 32 and the second interface 33 are respectively arranged at opposite ends of the U-shaped cavity, one end of the connecting tube 302 is connected to the first joint 1 through the first interface 32, and the other end of the connecting tube 302 is connected to the second joint 2 through the second interface 33; it can be understood that the connecting tube 302 spans between the first interface 32 and the second interface 33, the U-shaped cavity is located below the first interface 32, the second interface 33 and the connecting tube 302, and the first interface 32 and the second interface 33 are located at opposite ends of the connecting tube 302. In this embodiment, the sampling container 3 has a simple structure and low manufacturing cost.

[0085] The step of disassembling and separating the sampling container 3 from the first connector 1 and the second connector 2 and taking out the target solution in the containing cavity 31 (ie, step S400 ) includes:

[0086] The closed opposite ends of the U-shaped flexible tube 301 are controlled to be disassembled and separated from the first joint 1 and the second joint 2 respectively; it can be understood that the opposite ends of the U-shaped flexible tube 301 can be thermally combined and sealed by a thermal composite process, and the thermal composite section of the U-shaped flexible tube 301 can be disassembled, so that the opposite ends of the U-shaped flexible tube 301 can be disassembled from the first interface 32 and the second interface 33 respectively, and the disassembled U-shaped flexible tube 301 is in a closed state.

[0087] After connecting the two ends or one end of the U-shaped flexible tube 301, the target solution in the U-shaped cavity is controlled to be squeezed out. It can be understood that the port of the U-shaped flexible tube 301 is connected to an external container, and the target solution therein is squeezed into the external container by squeezing the U-shaped flexible tube 301, thereby completing the sampling of the target solution. In this embodiment, the user can easily carry the disassembled and separated U-shaped flexible tube 301, further improving the convenience of sampling the target solution.

[0088] In one embodiment, if Figure 1As shown, the sampling container 3 also includes a second tube clamp 303 and a third tube clamp 304, the second tube clamp 303 is used to clamp the pipeline between the first interface 32 and the accommodating chamber 31, and the third tube clamp 304 is used to clamp the pipeline between the second interface 33 and the accommodating chamber 31; it can be understood that when the second tube clamp 303 clamps the U-shaped flexible tube 301 between the first interface 32 and the accommodating tube 35, and the third tube clamp 304 clamps the U-shaped flexible tube 301 between the second interface 33 and the accommodating tube 35, the opposite ends of the U-shaped flexible tube 301 are in a closed state, and the closed state of the U-shaped flexible tube 301 clamped by the second tube clamp 303 and the third tube clamp 304 cannot be maintained for too long.

[0089] The controlling of closing the pipelines between the first interface 32 and the accommodating chamber 31 and between the second interface 33 and the accommodating chamber 31 (ie, step S200 ) includes:

[0090] After controlling the second tube clamp 303 to clamp the pipeline between the first interface 32 and the accommodating chamber 31 and heat-seal it, and controlling the third tube clamp 304 to clamp the pipeline between the second interface 33 and the accommodating chamber 31 and heat-seal it, it is confirmed that the pipeline between the first interface 32 and the accommodating chamber 31 and between the second interface 33 and the accommodating chamber 31 are closed. It can be understood that firstly, the pipeline of the U-shaped flexible tube 301 between the first interface 32 and the accommodating chamber 31 is clamped by the second tube clamp 303, and the pipeline of the U-shaped flexible tube 301 between the second interface 33 and the accommodating chamber 31 is clamped by the third tube clamp 304, and then the pipeline between the second tube clamp 303 and the first interface 32 is heat-sealed by the heat sealing machine, and the pipeline between the third tube clamp 304 and the second interface 33 is heat-sealed by the heat sealing machine; thereby completing the sealing work of the pipeline between the first interface 32 and the accommodating chamber 31, and completing the sealing work of the pipeline between the second interface 33 and the accommodating chamber 31. In this embodiment, before heat-sealing the pipeline between the first interface 32 and the accommodating chamber 31, the second tube clamp 303 is used to clamp the pipeline between the first interface 32 and the accommodating chamber 31; and before heat-sealing the pipeline between the second interface 33 and the accommodating chamber 31, the third tube clamp 304 is used to clamp the pipeline between the second interface 33 and the accommodating chamber 31, thereby avoiding the accident of leakage of the target solution in the accommodating chamber 31 when heat-sealing the pipeline.

[0091] The above are merely embodiments of the sampler and sampling method of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A sampler, characterized in that: It comprises a first joint, a second joint and a sampling container, wherein the sampling container is provided with a receiving cavity, a first interface and a second interface; one end of the first joint is connected to an external cell container, and the other end of the first joint is connected to the receiving cavity and the second interface respectively through the first interface; one end of the second joint is connected to a first external liquid extractor, and the other end of the second joint is connected to the receiving cavity and the first interface respectively through the second interface; The first external liquid extractor is used to extract the target solution in the external cell container into the containing cavity through the first joint and the first interface in sequence; The first external liquid extractor is further used to push the target solution remaining in the second joint, the first joint, and the pipe between the first joint and the second joint back into the external cell container after the pipes between the first interface and the accommodating chamber and between the second interface and the accommodating chamber are closed; The sampling container includes a U-shaped flexible tube and a connecting tube, the accommodating chamber is a U-shaped cavity arranged on the U-shaped flexible tube, the first interface and the second interface are respectively arranged at opposite ends of the U-shaped cavity, one end of the connecting tube is connected to the first joint through the first interface, and the other end of the connecting tube is connected to the second joint through the second interface.

2. The sampler according to claim 1, characterized in that: The sampling container further includes a second tube clamp and a third tube clamp, wherein the second tube clamp is used to clamp the pipeline between the first interface and the U-shaped cavity, and the third tube clamp is used to clamp the pipeline between the second interface and the U-shaped cavity; After controlling the second pipe clamp to clamp the pipe between the first interface and the accommodating cavity and heat-sealing the pipe, the pipe between the first interface and the accommodating cavity is sealed; After the third pipe clamp is controlled to clamp the pipe between the second interface and the accommodating cavity and heat-seals it, the pipe between the second interface and the accommodating cavity is closed.

3. The sampler according to claim 1 or 2, characterized in that: The sampling container is provided with scale lines for marking the volume of the target solution in the containing cavity.

4. The sampler according to claim 1 or 2, characterized in that: The sampler further comprises a first protective cap and a second protective cap, wherein the first protective cap is detachably covered on an end of the first joint away from the sampling container, and the second protective cap is detachably covered on an end of the second joint away from the sampling container.

5. The sampler according to claim 1 or 2, characterized in that: The sampler also includes a filter, which is installed at an end of the second joint away from the sampling container, and the first external liquid extractor is connected to the second joint through the filter; the filter is used to filter the gas entering the sampling container.

6. A sampling method of the sampler as claimed in claim 1, characterized in that: include: receiving a sampling instruction, and controlling the first external liquid extractor to extract the target solution in the external cell container into the containing cavity through the first joint and the first interface in sequence; Controlling the closure of the pipelines between the first interface and the accommodating chamber and between the second interface and the accommodating chamber; Controlling the first external liquid extractor to push the target solution remaining in the second joint, the first joint, and the pipeline between the first joint and the second joint back into the external cell container; Disassembling and separating the sampling container from the first joint and the second joint, and taking out the target solution in the containing cavity; The step of disassembling and separating the sampling container from the first joint and the second joint, and taking out the target solution in the containing cavity, comprises: Controlling the closed opposite ends of the U-shaped flexible tube to be disassembled and separated from the first interface and the second interface respectively; After connecting both ends or one end of the U-shaped flexible tube, the target solution in the U-shaped cavity is controlled to be squeezed out.

7. The sampling method according to claim 6, characterized in that: The sampling container further comprises a second tube clamp and a third tube clamp, wherein the second tube clamp is used to clamp the pipeline between the first interface and the accommodating cavity, and the third tube clamp is used to clamp the pipeline between the second interface and the accommodating cavity; The controlling the closure of the pipelines between the first interface and the accommodating chamber and between the second interface and the accommodating chamber comprises: After controlling the second tube clamp to clamp the pipeline between the first interface and the accommodating cavity and perform heat sealing treatment on it, and controlling the third tube clamp to clamp the pipeline between the second interface and the accommodating cavity and perform heat sealing treatment on it, it is confirmed that the pipelines between the first interface and the accommodating cavity and between the second interface and the accommodating cavity are closed.

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