A microbial strain extraction device and method

Abstract

This invention discloses a microbial strain extraction device, comprising: an extraction cylinder for extracting microbial strains from a petri dish; and a piston-type stretching mechanism that extends into the inner cavity of the extraction cylinder to adsorb the microbial strain solution from the petri dish into the extraction cylinder. The extraction cylinder includes a main cylinder and a secondary cylinder, both having hollow chambers. An outlet is connected to the connection between the main and secondary cylinders, and a movable sealing component is provided at the opening of the outlet. Under the action of an external driving force, the piston-type stretching mechanism reciprocates synchronously along the chambers of the main and secondary cylinders, driving the movable sealing component to slide along the inner cavity of the main cylinder to control the opening of the outlet. This allows the adsorbed microbial strain solution in the main cylinder to be introduced into the secondary cylinder through the outlet. The dual-cylinder configuration improves the extraction efficiency of the microbial strain solution, and the outlet remains closed under the control of the piston-type stretching mechanism, thus ensuring airtightness between the two chambers.

Description

Technical Field

[0001] This invention relates to the technical field of extraction devices, specifically to a microbial strain extraction device and method. Background Technology

[0002] A spawn refers to the propagation material composed of edible fungi mycelium and its growth substrate. It is a useful strain possessing certain capabilities, also known as seed preparation. Seed preparation refers to the process of cultivating a spawn under specific conditions into a pure production strain with a certain quantity and quality, ready for inoculation into petri dishes to further expand the cell volume and synthesize products. Typically, the spawn after cultivation in petri dishes needs to be extracted using an extraction device. During extraction, the airtightness of the extraction device must be ensured to prevent external air from affecting the spawn.

[0003] Currently available extraction devices mainly use single syringes for extraction. However, it is difficult to ensure the airtightness of the syringe during the extraction process, which can easily interfere with the extracted bacteria. Furthermore, the extraction efficiency of a single syringe is low, as it can only be used for one extraction. After extraction, the syringe must be removed for a second extraction. With the syringe being removed multiple times, the possibility of contamination increases. Summary of the Invention

[0004] The purpose of this invention is to provide a microbial strain extraction device to solve the technical problems of insufficient airtightness and low extraction efficiency of existing extraction devices.

[0005] To solve the above-mentioned technical problems, the present invention specifically provides the following technical solution:

[0006] A microbial strain extraction device, comprising,

[0007] Extraction tube, used to extract bacterial strains from petri dishes;

[0008] A piston-type stretching mechanism extends into the inner cavity of the extraction cylinder to adsorb the bacterial solution in the culture dish into the extraction cylinder;

[0009] The extraction cylinder includes a main cylinder and a secondary cylinder, both of which have hollow chambers. A liquid outlet is connected to the connection between the main cylinder and the secondary cylinder. A movable sealing component is provided at the opening of the liquid outlet. Under the action of an external driving force, the piston-type stretching mechanism reciprocates synchronously along the chambers in the main cylinder and the secondary cylinder, thereby driving the movable sealing component to slide along the inner cavity of the main cylinder to control the liquid outlet to close or shut down, so that the bacterial solution adsorbed in the main cylinder is introduced into the secondary cylinder through the liquid outlet.

[0010] As a preferred embodiment of the present invention, the inner cavity of the main cylinder is provided with a first chamber, and the inner cavity of the first chamber is slidably connected to the piston-type stretching mechanism so that the bacterial solution drawn into the first chamber flows into the secondary cylinder along the liquid outlet;

[0011] The inner cavity of the secondary cylinder is provided with a second chamber, which is slidably connected to the piston-type stretching mechanism to further adsorb the bacterial solution in the first chamber into the second chamber;

[0012] The auxiliary cylinder has a drain port and an air outlet connected to the side wall away from the main cylinder, and both the drain port and the air outlet are connected to the second chamber. The drain port and the air outlet are located on both sides of the drain port, and the air outlet is located on the right side of the drain port.

[0013] As a preferred embodiment of the present invention, both the drain port and the gas outlet are provided with one-way valves to facilitate the one-way flow of gas or liquid.

[0014] As a preferred embodiment of the present invention, the piston-type stretching mechanism includes a first piston rod, a second piston rod, and a connecting rod. The first piston rod and the second piston rod are respectively connected to both sides of the connecting rod. The connecting rod reciprocates under the action of an external driving force to synchronously drive the first piston rod and the second piston rod to move along the inner cavity of the extraction cylinder.

[0015] The first piston rod has a first sealing seat at one end that extends into the first chamber, and the second piston rod has a second sealing seat at one end that extends into the second chamber.

[0016] As a preferred embodiment of the present invention, when the second sealing seat moves to the side wall of the second chamber near the connecting rod, the second sealing seat just blocks the air outlet, and the first sealing seat pushes the movable sealing component to move to completely open the liquid outlet.

[0017] In a preferred embodiment of the present invention, the movable sealing assembly includes a movable plate slidably connected to the inner surface of the first chamber. The first chamber, located on the side of the liquid outlet, has a sliding groove that communicates with the liquid outlet. A guide rod is fixedly connected to the inner cavity of the sliding groove along its length. A guide hole, cooperating with the guide rod, is provided in the inner cavity of the movable plate. A telescopic spring, sleeved on the guide rod, connects the movable plate and the side wall of the sliding groove.

[0018] As a preferred embodiment of the present invention, the movable plate has an L-shaped structure, and the vertical end of the L-shaped mechanism is close to the side of the sliding groove, and the horizontal end of the L-shaped mechanism is provided with a protrusion, and the side wall of the liquid outlet is provided with a slot that cooperates with the protrusion.

[0019] The width of the protrusion is less than the distance between the protrusion and the end of the liquid outlet.

[0020] As a preferred embodiment of the present invention, a limiting rod is provided in the inner cavity of the slot along its own length direction, a sealing disc seat is slidably connected to the upper part of the limiting rod, and a return spring sleeved on the limiting rod is connected between the sealing disc seat and the slot.

[0021] The protruding inner cavity has a sliding hole that communicates with the guide hole, and the sliding hole is slidably engaged with the limiting rod.

[0022] In a preferred embodiment of the present invention, when the movable plate is completely disengaged from the slot, the sealing disc slides along the limiting rod under the elastic reset action of the reset spring to seal the opening of the slot.

[0023] As a preferred embodiment of the present invention, a method of using a microbial strain extraction device includes the following steps;

[0024] S100. Use a piston-type stretching mechanism to expel excess gas from the extraction tube, and then place the extraction tube into a petri dish.

[0025] S200 extracts the bacterial solution from the culture dish through the reciprocating motion of the piston-type stretching mechanism, and the piston-type stretching mechanism performs the dual processes of extraction and drainage during the reciprocating motion.

[0026] Compared with the prior art, the present invention has the following advantages:

[0027] This invention utilizes a dual-cylinder design to improve the extraction efficiency of bacterial solutions. The two dual-cylinder structures are connected by an outlet. During the extraction process of the piston-type stretching mechanism, the air pressure in the two chambers on both sides of the outlet decreases simultaneously. Before the outlet opens, the secondary cylinder is under negative pressure. Once the outlet opens, the negative-pressure secondary cylinder rapidly draws the solution from the main cylinder containing the bacterial solution, completing the rapid extraction of the bacterial strain. The bacterial solution is then discharged under the resetting action of the piston-type stretching mechanism. This device can perform single extractions, simply by removing the extraction cylinder, or it can perform cyclic extractions by keeping the extraction cylinder in the petri dish and reciprocating the piston-type stretching mechanism.

[0028] Meanwhile, the outlet opens during the movement of the piston-type stretching mechanism, making the two cylinders connected. Once the piston-type stretching mechanism is retracted, the outlet closes, ensuring that the two chambers remain relatively independent when no bacteria are being extracted, thereby improving the sealing of the extraction cylinder. Attached Figure Description

[0029] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.

[0030] Figure 1 This is a schematic diagram of the overall structure of the device provided by the present invention;

[0031] Figure 2 This is an enlarged structural diagram of part A in the device provided by the present invention;

[0032] Figure 3 A schematic diagram of the structure of the movable plate of the device provided by the present invention.

[0033] The labels in the diagram represent the following:

[0034] 1. Extraction cylinder; 2. Piston-type stretching mechanism; 3. Main cylinder; 4. Auxiliary cylinder; 5. Liquid outlet; 6. Movable sealing assembly; 7. First chamber; 8. Second chamber; 9. Drain outlet; 10. Air outlet; 11. One-way valve;

[0035] 21. First piston rod; 22. Second piston rod; 23. Connecting rod; 24. First sealing seat; 25. Second sealing seat;

[0036] 61. Movable plate; 62. Sliding groove; 63. Guide rod; 64. Guide hole; 65. Telescopic spring; 66. Protrusion; 67. Slot; 68. Limiting rod; 69. Sealing disc seat; 610. Return spring; 611. Sliding hole. Detailed Implementation

[0037] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] like Figure 1-2 As shown, a microbial strain extraction device includes an extraction cylinder 1 and a piston-type stretching mechanism 2.

[0039] Extraction tube 1 is used to extract bacterial strains from petri dishes;

[0040] Piston-type stretching mechanism 2 extends into the inner cavity of extraction cylinder 1 to adsorb the bacterial solution in the culture dish into extraction cylinder 1;

[0041] The extraction cylinder 1 includes a main cylinder 3 and a secondary cylinder 4. Both the main cylinder 3 and the secondary cylinder 4 have hollow chambers. The connection between the main cylinder 3 and the secondary cylinder 4 is connected to a liquid outlet 5. A movable sealing component 6 is provided at the opening of the liquid outlet 5. Under the action of external driving force, the piston-type stretching mechanism 2 reciprocates synchronously along the chambers in the main cylinder 3 and the secondary cylinder 4 to drive the movable sealing component 6 to slide along the inner cavity of the main cylinder 3 to control the liquid outlet 5 to close or shut down, so that the bacterial solution adsorbed in the main cylinder 3 is introduced into the secondary cylinder 4 through the liquid outlet 5.

[0042] This device improves the extraction efficiency of bacterial solutions through a dual-cylinder design. In use, the extraction cylinder 1 is directly inserted into the petri dish. During extraction, the piston-type stretching mechanism 2 draws the bacterial solution from the petri dish. The two dual-cylinder structures are connected by an outlet 5. During extraction by the piston-type stretching mechanism 2, the air pressure in the two chambers on both sides of the outlet 5 decreases simultaneously. Before the outlet 5 opens, the secondary cylinder 4 is under negative pressure. Once the outlet is opened, the negative-pressure secondary cylinder 4 rapidly draws in the solution from the main cylinder 3, completing the rapid extraction of the bacterial solution. The bacterial solution is then discharged under the resetting action of the piston-type stretching mechanism 2. This device can perform single extractions (simply removing the extraction cylinder) or cyclic extractions (keeping the extraction cylinder in the petri dish and reciprocating the piston-type stretching mechanism 2).

[0043] Meanwhile, during the extraction process of the piston-type stretching mechanism 2, the gas in the dual chambers never comes into contact with the external gas, and when extracting the bacterial solution, excess gas in the dual chambers can also be discharged, further improving the airtightness of the device.

[0044] During extraction, the main cylinder 3 and the auxiliary cylinder 4 mainly serve to store the bacterial solution. During the extraction process of the piston-type stretching mechanism 2, the pressure in the two chambers of the main cylinder 3 and the auxiliary cylinder 4 is constantly changing. Once the piston-type stretching mechanism 2 moves, the pressure on both sides of the chambers in the main cylinder 3 and the auxiliary cylinder 4 will also be different. In order to better facilitate the movement of the piston-type stretching mechanism 2, it is necessary to depressurize the main cylinder 3 and the auxiliary cylinder 4.

[0045] Specifically, such as Figure 1 As shown, the inner cavity of the main cylinder 3 is provided with a first chamber 7, and the inner cavity of the first chamber 7 is slidably connected to the piston-type stretching mechanism 2 so that the bacterial solution sucked into the first chamber 7 flows into the auxiliary cylinder 4 through the liquid outlet 5.

[0046] The inner cavity of the auxiliary cylinder 4 is provided with a second chamber 8, which is slidably connected to the piston-type stretching mechanism 2 to further adsorb the bacterial solution in the first chamber 7 into the second chamber 8.

[0047] Among them, the auxiliary cylinder 4 is connected to a drain port 9 and an air outlet 10 on the side wall away from the main cylinder 3, and both the drain port 9 and the air outlet 10 are connected to the second chamber 8. The drain port 9 and the air outlet 10 are located on the two sides of the drain port 5, and the air outlet 10 is located on the right side of the drain port 9.

[0048] During the movement of the piston-type stretching mechanism 2, in the initial stage, the piston-type stretching mechanism 2 moves synchronously in the first chamber 7 and the second chamber 8. The first chamber 7 draws in the bacterial solution from the culture dish, and the vent 10 in the second chamber 8 is depressurized under the action of the piston-type stretching mechanism 2, allowing excess gas in the second chamber 8 to be discharged. The pressure on the same side of the first chamber 7 and the second chamber 8 maintains the same change. This further ensures that when the piston-type stretching mechanism 2 is not in contact with the movable sealing component 6, the movable sealing component 6 is in a state of sealing the liquid outlet 5. Once the piston-type stretching mechanism 2 contacts the movable sealing component 6 and drives the movable sealing component 6 to move, the liquid outlet 5 is gradually opened, and the bacterial solution in the first chamber 7 quickly enters the second chamber 8 under negative pressure, completing the sampling.

[0049] During the reciprocating motion of the piston-type stretching mechanism 2, the bacterial solution and excess gas in the second chamber 8 must be kept out without entering.

[0050] Specifically, such as Figure 1 As shown, both the drain port 9 and the gas outlet 10 are equipped with one-way valves to facilitate the one-way flow of gas or liquid.

[0051] After the inoculum solution enters the first chamber 7, as the piston-type stretching mechanism 2 continues to advance, the volume of the inoculum solution entering the first chamber 7 will also increase. In order to avoid gas flow between the first chamber 7 and the second chamber 8, it is necessary to ensure the sealing between the first chamber 7 and the second chamber 8 in the early stage of the piston-type stretching mechanism 2, so as to avoid interference between the gases in the two chambers, thereby ensuring the quality of the inoculum solution in the second chamber 8.

[0052] Specifically, such as Figure 1-2 As shown, the movable sealing assembly 6 includes a movable plate 61, which is slidably connected to the inner surface of the first chamber 7. The first chamber 7 located on the side of the liquid outlet 5 has a sliding groove 62, which is connected to the liquid outlet 5. A guide rod 63 is fixedly connected to the inner cavity of the sliding groove 62 along its own length direction. A guide hole 64 that cooperates with the guide rod 63 is opened in the inner cavity of the movable plate 61. A telescopic spring 65 sleeved on the guide rod 63 is connected between the movable plate 61 and the side wall of the sliding groove 62.

[0053] In the initial state, the movable plate 61 is tightly pressed against the side wall of the outlet 5 by the elastic compression of the telescopic spring 65, thus sealing the outlet 5. When the piston-type tensioning mechanism 2 contacts, it pushes the movable plate 61 to move along the guide rod 63. At this time, the telescopic spring 65 is compressed and generates elasticity until the outlet 5 is fully opened, and the bacterial solution flows rapidly into the second chamber 8 through the outlet 5. When the piston-type tensioning mechanism 2 resets, the compressed telescopic spring 65 also resets and pushes the movable plate 61 to move along the guide rod 63 until the outlet 5 is sealed again.

[0054] During the process of the movable plate 61 being tightly attached to the liquid outlet 5, there is a gap between the two. In order to reduce the occurrence of the gap;

[0055] Specifically, the movable plate 61 has an L-shaped structure, and the vertical end of the L-shaped mechanism is close to the side of the sliding groove 62. The horizontal end of the L-shaped mechanism is provided with a protrusion 66, and the side wall of the liquid outlet 5 is provided with a slot 67 that cooperates with the protrusion 66.

[0056] The width of protrusion 66 is less than the distance between protrusion 66 and the end of outlet 5.

[0057] This ensures that when the movable plate 61 is in contact with the liquid outlet 5, the movable plate 61 can be accurately inserted into the slot 67. The protrusion 66 and the slot 67 form a stepped structure, which is used to improve the sealing between the movable plate 61 and the liquid outlet 5.

[0058] As the movable plate 61 reciprocates in the piston-type stretching mechanism 2, the protrusion 66 and the slot 67 will also be in a disengaged state. Once the protrusion 66 moves away from the slot 67, the slot 67 will connect with the liquid outlet 5, and the bacterial solution in the liquid outlet 5 will also flow into the slot 67. This not only wastes the bacterial solution, but also causes the residual bacterial solution to interfere with the next extraction. Therefore, it is necessary to ensure that when the protrusion 66 and the slot 67 are separated, the open end of the slot 67 should be kept in a closed state.

[0059] Specifically, such as Figure 2 As shown, a limiting rod 68 is provided in the inner cavity of the slot 67 along its own length direction. A sealing disc seat 69 is slidably connected to the upper part of the limiting rod 68. A return spring 610 sleeved on the limiting rod 68 is connected between the sealing disc seat 69 and the slot 67.

[0060] The inner cavity of the protrusion 66 is provided with a sliding hole 611 that communicates with the guide hole 64. The sliding hole 611 is slidably engaged with the limiting rod 68.

[0061] In the initial state, the protrusion 66 on the movable plate 61 is inserted into the slot 67, and the protrusion 66 presses against the sealing disc seat 69. At this time, the sealing disc seat 69 presses against the return spring 610 and causes the return spring 610 to undergo elastic deformation. Since both the protrusion 66 and the sealing disc seat 69 are slidably inserted into the limiting rod 68, during the process of the piston-type tensioning mechanism 2 pushing the movable plate 61 to move, the compressed return spring 610 pushes the sealing disc seat 69 to move along the limiting rod 68 under the action of elastic deformation until the sealing disc seat 69 seals the opening of the slot 67.

[0062] When the movable plate 61 is completely disengaged from the slot 67, the sealing disc seat 69 slides along the limiting rod under the elastic reset action of the reset spring 610 to seal the opening of the slot 67.

[0063] When extracting the bacterial solution, the piston-type stretching mechanism 2 moves synchronously in the first chamber 7 and the second chamber 8, which ensures that the pressure changes in the two chambers are the same, thus facilitating the rapid extraction of the bacterial solution.

[0064] Specifically, such as Figure 1 As shown, the piston-type stretching mechanism 2 includes a first piston rod 21, a second piston rod 22 and a connecting rod 23. The first piston rod 21 and the second piston rod 22 are respectively connected to both sides of the connecting rod 23. The connecting rod 23 reciprocates under the action of an external driving force to synchronously drive the first piston rod 21 and the second piston rod 22 to move along the inner cavity of the extraction cylinder 1.

[0065] The first piston rod 21 is connected to a first sealing seat 24 at one end that extends into the first chamber 7, and the second piston rod 22 is connected to a second sealing seat 25 at one end that extends into the second chamber 8.

[0066] An external driving force drives the connecting rod 23 to move. Since the connecting rod 23 is connected to both the first piston rod 21 and the second piston rod 22, the movement trends of the first piston rod 21 and the second piston rod 22 are the same as those of the connecting rod 23. Under the drive of the external driving force, the connecting rod 23 drives the first piston rod 21 and the second piston rod 22 to move synchronously along the extraction cylinder 1, thereby achieving the effect of extracting the bacterial solution.

[0067] Furthermore, the first piston rod 21 moves along the first chamber 7, and the second piston rod 22 moves along the second chamber 8. Under the action of the first sealing seat 24 and the second sealing seat 25, the pressure in the first chamber 7 and the second chamber 8 is adjusted. Once the first sealing seat 24 contacts the movable plate 61 and drives the movable plate 61 to move, the liquid outlet 5 will open. Since the second sealing seat 25 has reduced the pressure in the second chamber 8 to a negative pressure state, the bacterial solution in the first chamber 7 flows rapidly into the second chamber through the liquid outlet 5, which improves the efficiency of bacterial solution extraction.

[0068] It is understood that the external driving force for the piston-type stretching mechanism 2 can be manually driven, hydraulically driven, or point-driven. Preferably, the device is electrically driven, i.e., an electric telescopic rod is used. One end of the telescopic rod is fixed to the extraction cylinder, and the power output end of the other end is connected to the connecting rod.

[0069] When the second sealing seat 25 moves to the side wall of the second chamber 8 near the connecting rod 23, the second sealing seat 25 just blocks the air outlet 10, and the first sealing seat 24 pushes the movable sealing assembly 6 to move so as to fully open the liquid outlet 5.

[0070] Specifically, a method of using a microbial strain extraction device includes the following steps;

[0071] S100. Use the piston-type stretching mechanism to expel the excess gas from the extraction tube, and then place the extraction tube into the petri dish.

[0072] S200 extracts the bacterial solution from the culture dish through the reciprocating motion of the piston-type stretching mechanism, and the piston-type stretching mechanism performs the dual processes of extraction and drainage during the reciprocating motion.

[0073] The above embodiments are merely exemplary embodiments of this application and are not intended to limit this application. The scope of protection of this application is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to this application within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this application.

Claims

1. A microbial strain extraction device, characterized in that, include, Extraction tube (1) is used to extract bacterial strains from petri dishes; A piston-type stretching mechanism (2) extends into the inner cavity of the extraction cylinder (1) to adsorb the bacterial solution in the culture dish into the extraction cylinder (1); The extraction cylinder (1) includes a main cylinder (3) and a secondary cylinder (4). Both the main cylinder (3) and the secondary cylinder (4) have hollow chambers. The connection between the main cylinder (3) and the secondary cylinder (4) is connected to a liquid outlet (5). A movable sealing assembly (6) is provided at the opening of the liquid outlet (5). Under the action of external driving force, the piston-type stretching mechanism (2) moves synchronously along the chambers in the main cylinder (3) and the secondary cylinder (4) to drive the movable sealing assembly (6) to slide along the inner cavity of the main cylinder (3) to control the liquid outlet (5) to close or shut down, so that the bacterial solution adsorbed in the main cylinder (3) is introduced into the secondary cylinder (4) through the liquid outlet (5). The main cylinder (3) has a first chamber (7) in its inner cavity. The inner cavity of the first chamber (7) is slidably connected to the piston-type stretching mechanism (2) so that the bacterial solution sucked into the first chamber (7) flows into the secondary cylinder (4) through the outlet (5). The inner cavity of the auxiliary cylinder (4) is provided with a second chamber (8), and the second chamber (8) is slidably connected to the piston-type stretching mechanism (2) so as to further adsorb the bacterial solution in the first chamber (7) into the second chamber (8); Among them, the auxiliary cylinder (4) is connected to a drain port (9) and an air outlet (10) on the side wall away from the main cylinder (3), and the drain port (9) and the air outlet (10) are connected to the second chamber (8). The drain port (9) and the air outlet (10) are located on both sides of the drain port (5), and the air outlet (10) is located on the right side of the drain port (9). The inner cavities of the drain port (9) and the air outlet (10) are both equipped with one-way valves (11) to facilitate the one-way flow of gas or liquid; During the extraction process of the piston-type stretching mechanism (2), the gas in the dual chambers never comes into contact with the external gas; The piston-type stretching mechanism (2) includes a first piston rod (21), a second piston rod (22) and a connecting rod (23). The first piston rod (21) and the second piston rod (22) are respectively connected to both sides of the connecting rod (23). The connecting rod (23) reciprocates under the action of an external driving force to synchronously drive the first piston rod (21) and the second piston rod (22) to move along the inner cavity of the extraction cylinder (1). The first piston rod (21) is connected to a first sealing seat (24) at one end that extends into the first chamber (7), and the second piston rod (22) is connected to a second sealing seat (25) at one end that extends into the second chamber (8).

2. The microbial strain extraction device according to claim 1, characterized in that, When the second sealing seat (25) moves to the side wall of the second chamber (8) near the connecting rod (23), the second sealing seat (25) just blocks the air outlet (10), and the first sealing seat (24) pushes the movable sealing assembly (6) to move to completely open the liquid outlet (5).

3. The microbial strain extraction device according to claim 2, characterized in that, The movable sealing assembly (6) includes a movable plate (61), which is slidably connected to the inner surface of the first chamber (7). The first chamber (7) located on the side of the liquid outlet (5) has a sliding groove (62) and the sliding groove (62) is connected to the liquid outlet (5). A guide rod (63) is fixedly connected to the inner cavity of the sliding groove (62) along its own length direction. A guide hole (64) that cooperates with the guide rod (63) is opened in the inner cavity of the movable plate (61). A telescopic spring (65) sleeved on the guide rod (63) is connected between the movable plate (61) and the side wall of the sliding groove (62).

4. The microbial strain extraction device according to claim 3, characterized in that, The movable plate (61) has an L-shaped structure, and the vertical end of the L-shaped structure is close to the side of the sliding groove (62). The horizontal end of the L-shaped mechanism is provided with a protrusion (66), and the side wall of the liquid outlet (5) is provided with a slot (67) that cooperates with the protrusion (66). The width of the protrusion (66) is less than the distance between the protrusion (66) and the end of the outlet (5).

5. The microbial strain extraction device according to claim 4, characterized in that, The inner cavity of the slot (67) is provided with a limiting rod (68) along its own length direction. A sealing disc seat (69) is slidably connected to the upper part of the limiting rod (68). A return spring (610) sleeved on the limiting rod (68) is connected between the sealing disc seat (69) and the slot (67). The inner cavity of the protrusion (66) is provided with a sliding hole (611) that communicates with the guide hole (64), and the sliding hole (611) is slidably engaged with the limiting rod (68).

6. The microbial strain extraction device according to claim 5, characterized in that, When the movable plate (61) is completely disengaged from the slot (67), the sealing disc seat (69) slides along the limiting rod under the elastic reset action of the reset spring (610) to seal the opening of the slot (67).

7. The method of using the microbial strain extraction device as described in any one of claims 1-6, characterized in that: Includes the following steps; S100. Use the piston-type stretching mechanism to expel the excess gas from the extraction tube, and then place the extraction tube into the petri dish. S200 extracts the bacterial solution from the culture dish through the reciprocating motion of the piston-type stretching mechanism, and the piston-type stretching mechanism performs the dual processes of extraction and drainage during the reciprocating motion.

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