Detection apparatus and system based on multiple excitation light sources

Abstract

The application provides a detection device and system based on multiple excitation light sources, comprising an image acquisition module, a light source module, a light source and a detection table; the light source module comprises an integrating sphere shell, the integrating sphere shell is provided with an image acquisition window, a light source passing hole and an observation window, the image acquisition module is located at the image acquisition window of the integrating sphere shell, the light source is arranged close to the light source passing hole, the light beam emitted by the light source passes through the light passing hole to reach the inside of the integrating sphere shell, and the light beam is diffusely reflected on the inner wall of the integrating sphere shell, and the detection table is located at the observation window of the integrating sphere shell. The light source module solves the super-high requirement of uniform light path required by such an optical detection structure, and then more accurate identification and interpretation can be carried out, the sensitivity of detection is improved, and the defect of low interpretation sensitivity of other detection equipment is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of detection, in particular to a detection device and system based on multiple excitation light sources. BACKGROUND

[0002] The fluorescent immunochromatography technology is a new quantitative detection technology combining the immunofluorescence technology and the traditional immunochromatography technology. The technology takes a strip-shaped fiber chromatography material with a detection line (coated antibody or coated antigen) and a quality control line (anti-antibody) fixed as a stationary phase, a test solution as a mobile phase, and a fluorescently labeled antibody or antigen fixed on a connecting pad. The analyte is moved on the chromatography strip through capillary action. For macromolecular antigens (proteins, viruses, pathogenic bacteria, etc.) with multiple antigenic determinants, a "sandwich" type double-antibody sandwich immunochromatography method is usually used, that is, the analyte is combined with the fluorescently labeled antibody under the action of the mobile phase, and then combined with the coated antibody to form a "sandwich" type double-antibody sandwich when reaching the detection line. Finally, the concentration of the analyte can be quantitatively analyzed through the fluorescence intensity of the detection reaction of the quality control line and the detection line. The technology not only retains the advantages of the colloidal gold immunochromatography technology, such as simple operation, rapid detection, and strong portability, but also realizes accurate quantification of the detection results through fluorescence tracing enhancement technology. The fluorescent immunochromatography detection technology has been widely used in many fields such as clinical immunology index testing and environmental pathogenic microorganism detection.

[0003] At present, the fluorescent immunochromatography detection technology mainly detects a single target, that is, one test strip and one sample addition can only detect one item. The fluorescent immunochromatography detection technology has very high requirements for uniform light path, which has a great influence on the detection results, and therefore a detection device with uniform and high-precision light path is needed. SUMMARY

[0004] In view of the defects in the prior art, the purpose of the present application is to provide a detection device and system based on multiple excitation light sources.

[0005] The detection device based on multiple excitation light sources provided by the present application comprises an image acquisition module, a light source module, a light source, and a detection table. The light source module comprises an integrating sphere shell, and the integrating sphere shell is provided with an image acquisition window, a light source passing hole, and an observation window. The image acquisition module is located at the image acquisition window of the integrating sphere shell, and the light source is arranged close to the light source passing hole. The light beam emitted by the light source passes through the light passing hole to the inside of the integrating sphere shell, and occurs diffuse reflection on the inner wall of the integrating sphere shell. The detection table is located at the observation window of the integrating sphere shell, and the diffusely reflected light uniformly irradiates on the detection table through the observation window.

[0006] Preferably, the system further includes a filter module, which includes a filter and a filter switching module. The filter is slidably disposed between the image acquisition window and the image acquisition module, and the filter switching module drives the filter to install and remove the filter.

[0007] Preferably, the filter switching module includes a filter holder, a switching linkage, and a linkage guide seat; the filter holder is slidably disposed between the image acquisition module and the acquisition window, and the filter holder is provided with a direct light transmission hole and a filter hole respectively, and the filter is installed in the filter hole, allowing direct light transmission or filtering by the filter according to the detection requirements; the filter holder is connected to the switching linkage through a connector, and the switching linkage is slidably disposed in the linkage guide seat.

[0008] Preferably, the image acquisition module uses a photographing or image scanning mode to perform image recognition on the test object on the testing platform.

[0009] Preferably, the light source is provided in multiple sets, including white LEDs and ultraviolet LEDs. The light emission state of the light source is adjusted by controlling the current of the LED beads through software. Multiple light sources are provided through corresponding holes.

[0010] Preferably, the light source further includes a heat sink, which is installed on the back of the ultraviolet LED light-emitting panel, with the front of the ultraviolet LED light-emitting panel facing the light source through a hole.

[0011] The present invention provides a detection system for multiple excitation light sources, comprising one or more modular combinations of the above-mentioned detection devices based on multiple excitation light sources.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] 1. This light source module solves the ultra-high requirement of uniform optical path for this type of optical detection structure, thereby enabling more accurate identification and interpretation, improving detection sensitivity, and overcoming the shortcomings of low interpretation sensitivity in other detection devices.

[0014] 2. By replacing the LED beads and filters in this light source module, both conventional immunochromatography and fluorescence immunochromatography can be integrated into one device for detection. Furthermore, by changing the LED light source, other similar detection methods can be achieved, overcoming the shortcomings of other devices that rely on a single detection method.

[0015] 3. The modular design allows for easy addition of modules to increase detection throughput, enabling simultaneous detection of multiple channels and methods, thus overcoming the shortcomings of other detection equipment, such as low detection throughput or lack of scalability. Attached Figure Description

[0016] Other features, objects, and advantages of the application will become apparent from the following detailed description of non-limiting embodiments, when read in connection with the following accompanying drawings:

[0017] Figure 1 is a whole sectional view of the detection device of the present application;

[0018] Figure 2 is a schematic diagram of the light source module in the detection device of the present application;

[0019] Figure 3 is a sectional view of the light source module in the detection device of the present application;

[0020] Figure 4 is a schematic diagram of the whole structure of the detection device of the present application;

[0021] Figure 5 is a sectional view of the light filtering module in the detection device of the present application.

[0022] Explanation of reference signs:

[0023] image acquisition module 1 detection table 4

[0024] light source module 2 light filter 51

[0025] integrating sphere shell 21 light filter holder 52

[0026] image acquisition window 22 switching connecting rod 53

[0027] light source passing hole 23 connecting rod guide seat 54

[0028] observation window 24 direct light transmission hole 55

[0029] white light LED 31 light filtering hole 56

[0030] ultraviolet LED 32 heat dissipation fin 6 DETAILED DESCRIPTION

[0031] The present application will be described in detail below with specific embodiments. The following embodiments will help those skilled in the art to further understand the present application, but do not limit the present application in any form. It should be noted that for those skilled in the art, without departing from the concept of the present application, a number of changes and improvements can be made. These are within the scope of the present application.

[0032] The present application discloses a detection device based on multiple excitation light sources, referring to Figures 1-4 , comprising:

[0033] Image acquisition module 1: using the mode of taking pictures or image scanning to identify the C line strength on the reagent card by image recognition method for interpretation.

[0034] Light source module 2: using similar integral sphere structure form in the processing technology using diffuse reflection extinction treatment so that the light in the inner wall of multiple reflections to form a uniform illumination to obtain higher measurement accuracy.

[0035] Light source: provide the light needed for detection;

[0036] Detection platform 4: for placing the measured object.

[0037] The light source module 2 includes an integral sphere shell 21, which is provided with an image acquisition window 22, a light source passing hole 23 and an observation window 24. The image acquisition module 1 is located at the image acquisition window 22 of the integral sphere shell 21, and the light source is arranged near the light source passing hole 23. The light beam emitted by the light source passes through the light passing hole to the inside of the integral sphere shell 21, and the diffuse reflection occurs on the inner wall of the integral sphere shell 21. The detection platform 4 is located at the observation window 24 of the integral sphere shell 21, and the diffusely reflected light uniformly irradiates on the detection platform 4 through the observation window 24. The integral sphere shell 21 can reduce and remove the measurement error caused by the shape, divergence angle of the light and the response difference of the different positions on the detector.

[0038] An optical filter module is further arranged between the image acquisition window 22 and the image acquisition module 1, and a filter 51 can be automatically added when special light source interpretation is performed. The optical filter module includes a filter 51 and a filter 51 switching module. The filter 51 is slidably arranged between the image acquisition window 22 and the image acquisition module 1, and the filter 51 switching module drives the filter 51 to realize the installation and removal of the filter 51. The filter 51 switching module includes a filter holder 52, a switching connecting rod 53 and a connecting rod guide seat 54. The filter holder 52 is slidably arranged between the image acquisition module 1 and the acquisition window, and the filter holder 52 is respectively provided with a direct light transmission hole 55 and a filter hole 56. The filter 51 is installed in the filter hole 56, and direct light transmission or filtering through the filter 51 is selected according to the detection requirement. The filter holder 52 is connected with the switching connecting rod 53 through a connecting piece, which can be a bolt in this embodiment. The switching connecting rod 53 is slidably arranged in the connecting rod guide seat 54. A threaded hole is axially formed in the end of the switching connecting rod 53, and the switching connecting rod 53 can be moved by a motor, so that the electric control is realized. The manual adjustment is also possible.

[0039] The working principle of the filter module is that the switching connecting rod 53 is manually or electrically pushed to move left and right, as shown in the figure, the switching connecting rod 53 and the filter frame 52 are fixed by screws, the switching connecting rod 53 moves left and right in the connecting rod guide seat 54 to drive the filter frame 52 to move left and right, the filter 51 is placed in the filter frame 52, when moving to the rightmost side, it can be seen that there is no filter 51 below the camera, the light can be directly connected to the camera through the filter frame 52, and in this case, the white light source is convenient for the camera to directly shoot the reagent card reaction result.

[0040] When the switching connecting rod 53 is manually or electrically moved to the leftmost side, the filter 51 is directly moved to the front of the camera, so that it is suitable for the case of the ultraviolet LED 32, the excited light is captured by the camera through the filter 51, so that the reagent card reaction result can be more clearly shot.

[0041] The image acquisition module 1 adopts a photographing or image scanning mode to perform image recognition on the measured object on the detection table 4. The image acquisition module 1 in this scheme is a camera, but is not limited to a camera, and any photosensitive measuring element can be applicable to this mode.

[0042] The light source is provided with multiple groups, including a white light LED 31 and an ultraviolet LED 32, the light-emitting state of the light source is adjusted by software control of the size of the lamp bead current, the lamp bead current is adjusted in size by software, and then the brightness of the light source is adjusted, so that the stability and uniformity of the brightness of the light source are ensured. The light source is correspondingly provided with multiple light source passing holes 23. In actual measurement, the light source is adjusted according to the type of detection and the light intensity requirement. The light source also includes a heat sink 6, the heat sink 6 is installed on the back of the ultraviolet LED 32 light-emitting lamp plate, and the front of the ultraviolet LED 32 light-emitting lamp plate faces the light source passing hole 23. By replacing the types of LED lamp beads and the types of filters 51, the detection requirements under different spectral adjustments can be achieved, and different detection methods can be adapted.

[0043] The application also discloses a detection system of multiple excitation light sources Figure 5 , which comprises one or more groups of detection devices based on multiple excitation light sources.

[0044] In the description of the present application, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the purpose of facilitating the description of the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0045] The specific embodiments of the present application are described above. It should be understood that the present application is not limited to the specific embodiments described above, and various changes or modifications can be made by those skilled in the art within the scope of the claims, which do not affect the essential content of the present application. The embodiments of the present application and the features in the embodiments can be arbitrarily combined with each other in the case of no conflict.

Claims

1. A detection device based on multiple excitation light sources, characterized in that, include: The system includes an image acquisition module, a light source module, a light source, and a detection stage. The light source module comprises an integrating sphere housing, on which an image acquisition window, a light source passage hole, and an observation window are provided. The image acquisition module is located at the image acquisition window of the integrating sphere housing, and the light source is positioned near the light source passage hole. The light beam emitted by the light source passes through the light source passage hole to reach the interior of the integrating sphere housing, where it undergoes diffuse reflection on the inner wall of the integrating sphere housing. The detection stage is located at the observation window of the integrating sphere housing, and the diffusely reflected light passes through the observation window and evenly illuminates the detection stage. It also includes a filter module, which includes a filter and a filter switching module. The filter is slidably disposed between the image acquisition window and the image acquisition module. The filter switching module drives the filter to realize the installation and removal of the filter. The filter switching module includes a filter holder, a switching linkage, and a linkage guide seat. The filter holder is slidably disposed between the image acquisition module and the image acquisition window. The filter holder has direct light transmission holes and filter holes respectively. The filter is installed in the filter holes, and direct light transmission or filtering by the filter is selected according to the detection requirements. The filter holder is connected to the switching linkage through a connector, and the switching linkage is slidably disposed in the linkage guide seat. The light source is provided in multiple sets, including white LEDs and ultraviolet LEDs. The light emission state of the light source is adjusted by controlling the current of the LED beads through software. Multiple light sources are provided through corresponding holes. The light source also includes a heat sink, which is installed on the back of the ultraviolet LED light-emitting panel, with the front of the ultraviolet LED light-emitting panel facing the light source through a hole.

2. The detection device based on multiple excitation light sources according to claim 1, characterized in that: The image acquisition module uses either photography or image scanning to perform image recognition on the object being tested on the testing platform.

3. A detection system with multiple excitation sources, characterized in that: It comprises a modular combination of one or more detection devices based on multiple excitation light sources as described in any one of claims 1-2.

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