Tissue dissociation assembly and device

Abstract

The application belongs to the technical field of tissue dissociation, and discloses a tissue dissociation assembly and device. The tissue dissociation assembly comprises a dissociation bottle body, a dissociation bottle cap and a rotor. The dissociation bottle body is used for containing samples. The bottom of the dissociation bottle body is provided with a first shearing part. The dissociation bottle cap is detachably connected with the dissociation bottle body. The first end of the rotor is rotationally connected with the dissociation bottle cap. The second end of the rotor extends to the bottom of the dissociation bottle body and is provided with a second shearing part. The tissue dissociation assembly provided by the application is characterized in that, when the rotor rotates forward, the first side of the first shearing part interacts with the second shearing part to cut the samples, and at the same time, the digestive enzymes in the digestive solution in the dissociation bottle body digest the samples. Similarly, when the rotor rotates reversely, the second side of the first shearing part interacts with the second shearing part to stir the samples, so that the samples and the digestive solution are fully mixed, which is beneficial to the digestion of the samples, effectively avoids cross contamination, and improves the digestion efficiency.

Description

Technical Field

[0001] This invention relates to the field of tissue dissociation technology, and more particularly to a tissue dissociation component and apparatus. Background Technology

[0002] Tissue dissociation is an indispensable and important step in cellular-level detection and a commonly used basic technique in biomedical laboratories.

[0003] In existing technologies, enzymatic tissue dissociation is the most universal dissociation method. The tissue inside the centrifuge tube is cut into small fragments using surgical scissors, then placed in a digestive solution, and the centrifuge tube is placed in a shaker for continuous shaking. The digestive solution contains digestive enzymes, which digest the tissue into a single-cell suspension at a reaction temperature of 37 degrees Celsius.

[0004] Currently, most laboratories manually complete a series of cutting and digestion steps. Cutting and digestion are carried out separately, which makes it difficult to solve the problem of cross-contamination of samples, reduces the efficiency of digestion, and increases the complexity of the experimental process. Summary of the Invention

[0005] One objective of this invention is to provide a tissue dissociation component that can simultaneously dissect and digest samples, effectively avoiding cross-contamination, improving digestion efficiency, and simplifying experimental operation.

[0006] To achieve this objective, the present invention adopts the following technical solution:

[0007] A tissue dissociation assembly is provided, the tissue dissociation assembly comprising:

[0008] A dissociation bottle body, the dissociation bottle body being used to hold samples, and a first shearing section being provided at the bottom of the dissociation bottle body;

[0009] The bottle cap is detachably connected to the detachable bottle body;

[0010] A rotor, the first end of which is rotatably connected to the detachable bottle cap, and the second end of which extends to the bottom of the detachable bottle and is provided with a second shearing section; wherein...

[0011] When the rotor rotates forward, the second shearing section intersects with the first shearing section, and the first side of the first shearing section interacts with the second shearing section to cut the sample;

[0012] When the rotor reverses, the second shearing section intersects with the first shearing section, and the second side of the first shearing section interacts with the second shearing section to stir the sample.

[0013] Optionally, the first shearing section includes an arc-shaped plate, the first side of which is a stepped blade, and the second side of which is a smooth surface;

[0014] The second shearing section is paddle-shaped, and a shearing groove is provided on the lower edge of the second shearing section; wherein,

[0015] When the rotor rotates forward, the stepped blade of the arc-shaped plate passes through the shearing groove before the smooth surface to cut the sample;

[0016] When the rotor reverses, the smooth surface of the arc-shaped plate passes through the shearing groove before the stepped blades to agitate the sample.

[0017] Optionally, multiple sets of arc-shaped plates are arranged radially along the dissociated bottle body, and the arc-shaped plates in each set are spaced apart circumferentially along the dissociated bottle body.

[0018] Optionally, the arc-shaped plates in each group are staggered along the radial direction of the dissociated bottle.

[0019] Another objective of this invention is to provide a tissue dissociation device that can automatically complete the shredding and digestion of samples, simplifying experimental procedures.

[0020] To achieve this objective, the present invention adopts the following technical solution:

[0021] A tissue dissociation apparatus is provided, the tissue dissociation apparatus comprising:

[0022] The above-mentioned tissue dissociation components;

[0023] case;

[0024] The fixing component includes a first linear module disposed within the housing and a fixing seat disposed on the first linear module. The first linear module can drive the fixing seat to move along a first direction to switch the fixing seat between a disengagement position within the housing and a feeding position outside the housing. The fixing seat is detachably connected to the disengagement bottle body.

[0025] The rotating assembly includes a second linear module disposed within the housing, a rotary drive device disposed on the second linear module, and a connector connected to the rotary drive device. The second linear module can drive the rotary drive device and the connector to move toward the tissue dissociation assembly located at the dissociation position so that the connector is connected to the rotor in a transmission connection. The rotary drive device can drive the rotor to rotate by driving the connector to rotate.

[0026] Optionally, the first linear module includes:

[0027] The first guide assembly includes a first slide rail mounted on the bottom of the housing and a first slider slidably connected to the first slide rail;

[0028] The first drive assembly includes a first drive motor mounted on the bottom of the housing, a first lead screw connected to the first drive motor, and a first threaded sleeve threadedly connected to the first lead screw. The first threaded sleeve is fixedly connected to the first slider and to the fixed base.

[0029] Optionally, the second linear module includes:

[0030] The second guide assembly includes a second slide rail mounted on the top of the housing and a second slider slidably connected to the second slide rail;

[0031] The second drive assembly includes a second drive motor mounted on the top of the housing, a second lead screw connected to the second drive motor, and a second threaded sleeve threadedly connected to the second lead screw. The second threaded sleeve is fixedly connected to the second slider and to the rotary drive device.

[0032] Optionally, one of the connector and the rotor is provided with a connecting groove, and the other is provided with a connecting shaft, wherein the connecting shaft can be inserted into the connecting groove to realize the transmission connection between the connector and the rotor.

[0033] Optionally, the fixing base is provided with a positioning cylinder, and a plurality of positioning protrusions are provided at circumferential intervals on the inner side of the positioning cylinder. The periphery of the dissociated bottle is provided with positioning grooves that correspond one-to-one with the positioning protrusions. The dissociated bottle can be placed inside the positioning cylinder and the positioning protrusions are placed inside the positioning grooves.

[0034] Optionally, multiple sets of both the fixing component and the rotating component are provided in a one-to-one correspondence along the second direction.

[0035] Beneficial effects:

[0036] The tissue dissociation assembly provided by this invention features a rotor that rotates clockwise, causing the second shearing section to rotate, resulting in an alternating motion between the second and first shearing sections. The first side of the first shearing section interacts with the second shearing section to cut the sample inside the dissociation bottle, while digestive enzymes in the digestive fluid inside the dissociation bottle digest the sample. Similarly, when the rotor rotates counterclockwise, it also causes the second shearing section to rotate, again resulting in an alternating motion between the second and first shearing sections. The second side of the first shearing section interacts with the second shearing section to stir the sample inside the dissociation bottle, ensuring thorough mixing of the sample and digestive fluid, which is beneficial for sample digestion. The simultaneous cutting and digestion of the sample inside the dissociation bottle effectively avoids cross-contamination caused by separating sample cutting and digestion, and improves digestion efficiency. The experimental operation is also simple.

[0037] The tissue dissociation device provided by this invention firstly moves the fixed seat along a first direction via a first linear module, transferring the fixed seat from the dissociation position to the loading position; secondly, the dissociation bottle is fixed on the fixed seat, thereby fixing the tissue dissociation assembly; thirdly, the fixed seat is moved along the first direction via the first linear module, transferring the fixed seat from the loading position to the dissociation position; finally, the rotary drive device and the connecting piece are moved toward the tissue dissociation assembly via a second linear module, so that the connecting piece is connected to the rotor drive. The rotary drive device drives the connecting piece to drive the rotor to rotate regularly in both forward and reverse directions, automatically completing the sample cutting, stirring, and digestion, simplifying experimental operations. Attached Figure Description

[0038] Figure 1 This is a schematic diagram of the internal structure of the tissue dissociation device provided in Embodiment 1 of the present invention from a certain perspective.

[0039] Figure 2 This is a schematic diagram of the internal structure of the tissue dissociation device provided in Embodiment 1 of the present invention from another perspective;

[0040] Figure 3 This is an exploded view of the tissue dissociation component provided in Embodiment 1 of the present invention;

[0041] Figure 4 This is a schematic diagram of the tissue dissociation device provided in Embodiment 1 of the present invention;

[0042] Figure 5 This is a cross-sectional view of the dissociative bottle body provided in Embodiment 1 of the present invention;

[0043] Figure 6 This is a schematic diagram of the structure of the dissociative bottle provided in Embodiment 1 of the present invention;

[0044] Figure 7 This is a schematic diagram of the structure of the dissociable bottle cap and rotor provided in Embodiment 1 of the present invention;

[0045] Figure 8 This is a schematic diagram of the structure of the fixing base provided in Embodiment 1 of the present invention;

[0046] Figure 9 This is an exploded view of the tissue dissociation assembly provided in Embodiment 2 of the present invention;

[0047] Figure 10 This is a schematic diagram of the structure of the dissociated bottle cap and rotor provided in Embodiment 2 of the present invention.

[0048] In the picture:

[0049] 100. Tissue dissociation assembly; 110. Dissociation bottle body; 111. First shearing section; 1111. Arc plate; 1112. Stepped blade; 1113. Smooth surface; 112. Positioning groove; 120. Dissociation bottle cap; 121. Sleeve; 122. First annular groove; 123. Second annular protrusion; 130. Rotor; 131. Second shearing section; 1311. Shearing groove; 1312. Paddle; 1313. Transition plate; 132. First annular protrusion; 133. First annular limiting plate; 1331. Sealing groove; 134. Second annular groove; 1341. Buckle; 135. Second annular limiting plate;

[0050] 200, Housing; 210, Mounting platform; 211, First bearing housing; 212, First position sensor; 220, Mounting plate; 221, Second bearing housing; 222, Second position sensor;

[0051] 300. Fixing component; 310. First linear module; 311. First slide rail; 312. First slider; 313. First drive motor; 314. First lead screw; 315. First threaded sleeve; 3151. First sensor; 320. Fixing base; 321. Baffle; 322. Positioning cylinder; 323. Positioning protrusion;

[0052] 400. Rotating assembly; 410. Second linear module; 411. Second slide rail; 412. Second slider; 413. Second drive motor; 414. Second lead screw; 415. Second threaded sleeve; 4151. Second sensor; 420. Rotary drive device; 430. Connector;

[0053] 510. Connecting groove; 520. Connecting shaft;

[0054] 610. Controller; 620. Control panel. Detailed Implementation

[0055] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and not intended to limit it. Furthermore, it should be noted that, for ease of description, the accompanying drawings show only the parts relevant to the present invention, and not all of the structures.

[0056] In the description of this invention, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0057] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0058] In the description of this embodiment, the terms "upper," "lower," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the present invention. In addition, the terms "first" and "second" are used only for distinction in description and have no special meaning.

[0059] Example 1

[0060] Reference Figures 1 to 4 As shown, this embodiment provides a tissue dissociation device, including a tissue dissociation component 100, a housing 200, a fixing component 300, and a rotating component 400.

[0061] Specifically, continue to refer to Figure 3 As shown, the tissue dissociation assembly 100 includes a dissociation bottle 110, a dissociation cap 120, and a rotor 130. The dissociation bottle 110 is used to hold samples, and a first shearing section 111 is provided at the bottom of the dissociation bottle 110. The dissociation cap 120 is detachably connected to the dissociation bottle 110. The first end of the rotor 130 is rotatably connected to the dissociation cap 120, and the second end of the rotor 130 extends to the bottom of the dissociation bottle 110 and is provided with a second shearing section 131. When the rotor 130 rotates clockwise, the second shearing section 131 and the first shearing section 111 intersect, and the first side of the first shearing section 111 interacts with the second shearing section 131 to cut the sample. When the rotor 130 rotates counterclockwise, the second shearing section 131 and the first shearing section 111 intersect, and the second side of the first shearing section 111 interacts with the second shearing section 131 to stir the sample.

[0062] In this embodiment, the rotor 130 rotates clockwise, driving the second shearing section 131 to rotate, so that the second shearing section 131 moves alternately relative to the first shearing section 111. The first side of the first shearing section 111 interacts with the second shearing section 131 to cut the sample in the dissociation bottle 110, while the digestive enzyme in the digestive liquid in the dissociation bottle 110 digests the sample. Similarly, when the rotor 130 rotates counterclockwise, it also drives the second shearing section 131 to rotate, so that the second shearing section 131 moves alternately relative to the first shearing section 111. The second side of the first shearing section 111 interacts with the second shearing section 131 to stir the sample in the dissociation bottle 110, so that the sample and digestive liquid are fully mixed, which is beneficial to the digestion of the sample. The cutting and digestion of the sample in the dissociation bottle 110 are carried out simultaneously, which effectively avoids the cross-contamination problem caused by the separate cutting and digestion of the sample, and improves the digestion efficiency. The experimental operation is simple.

[0063] Preferably, the dissociative bottle body 110 and the dissociative bottle cap 120 are threaded or tightly fitted together.

[0064] In this embodiment, reference is made to Figures 5 to 6 As shown, the first shearing part 111 includes an arc-shaped plate 1111, the first side of the arc-shaped plate 1111 is a stepped blade 1112, and the second side of the arc-shaped plate 1111 is a smooth surface 1113; the second shearing part 131 is paddle-shaped, and a shearing groove 1311 is provided on the lower edge of the second shearing part 131. In this embodiment, when the rotor 130 rotates forward, the second shearing section 131 and the first shearing section 111 intersect, and the stepped blade 1112 of the arc plate 1111 passes through the shearing groove 1311 before the smooth surface 1113. The stepped blade 1112 is equivalent to a saw tooth and cuts the sample at the shearing groove 1311. When the rotor 130 rotates in reverse, the smooth surface 1113 of the arc plate 1111 passes through the shearing groove 1311 before the stepped blade 1112. The paddle-shaped second shearing section 131 can stir the sample well, and the smooth surface 1113 has a turbulence effect, thereby making the sample and digestion liquid fully mixed and achieving the purpose of stirring the sample.

[0065] Specifically, the second shearing section 131 includes two blades 1312 with the same direction of rotation, and the two blades 1312 are connected as one unit by a transition plate 1313.

[0066] Specifically, multiple sets of arc-shaped plates 1111 are arranged radially along the dissociation bottle body 110, and each set of arc-shaped plates 1111 is arranged circumferentially along the dissociation bottle body 110 to further accelerate the cutting of the sample and the mixing of the sample and digestion solution.

[0067] Furthermore, the dimensions of the radially outer arc plate 1111 and the shear groove 1311 are larger than those of the inner arc plate 1111 and the shear groove 1311, so as to achieve better cutting and mixing effects.

[0068] Furthermore, the arc-shaped plates 1111 are arranged in a staggered manner along the radial direction of the dissociated bottle 110 to achieve segmented cutting and turbulence, thereby achieving better cutting and stirring effects and effectively reducing the torque on the rotor 130, so that the rotor 130 can rotate stably.

[0069] It is worth mentioning that after a set of arc-shaped plates 1111 passes through the corresponding shear groove 1311, another set of arc-shaped plates 1111 enters the corresponding shear groove 1311, which further improves the stability of the rotor 130 rotation and effectively avoids collision between the first shear section 111 and the second shear section 131.

[0070] Preferably, two sets of arc-shaped plates 1111 are arranged radially along the dissociated bottle body 110.

[0071] Furthermore, each set of curved plates 1111 includes two, and the two curved plates 1111 are set at 180°.

[0072] In this embodiment, reference continues to be made to... Figures 1 to 2 As shown, the fixing component 300 includes a first linear module 310 disposed within the housing 200 and a fixing seat 320 disposed on the first linear module 310. The first linear module 310 can drive the fixing seat 320 to move along a first direction to switch the fixing seat 320 between the disassembly position within the housing 200 and the feeding position outside the housing 200. The fixing seat 320 is detachably connected to the disassembly bottle 110. The rotating component 400 includes a second linear module 410 disposed within the housing 200, a rotary drive device 420 disposed on the second linear module 410, and a connector 430 connected to the rotary drive device 420. The second linear module 410 can drive the rotary drive device 420 and the connector 430 to move toward the tissue disassembly component 100 located at the disassembly position so that the connector 430 is connected to the rotor 130 in a transmission connection. The rotary drive device 420 can drive the rotor 130 to rotate by driving the connector 430 to rotate.

[0073] In this embodiment, firstly, the first linear module 310 drives the fixed seat 320 to move along the first direction, so that the fixed seat 320 is transferred from the dissociation position to the loading position; secondly, the dissociation bottle 110 is fixed on the fixed seat 320, thereby fixing the tissue dissociation assembly 100; thirdly, the first linear module 310 drives the fixed seat 320 to move along the first direction, so that the fixed seat 320 is transferred from the loading position to the dissociation position; finally, the second linear module 410 drives the rotary drive device 420 and the connector 430 to move toward the tissue dissociation assembly 100, so that the connector 430 is connected to the rotor 130 in a transmission connection. The rotary drive device 420 drives the connector 430 to drive the rotor 130 to rotate regularly in both directions, automatically completing the sample cutting, stirring and digestion, simplifying the experimental operation.

[0074] Preferably, the rotary drive device 420 is an electric motor.

[0075] It is worth mentioning that multiple sets of the fixing component 300 and the rotating component 400 are arranged in a one-to-one correspondence along the second direction, thereby enabling the simultaneous cutting, stirring, and digestion of multiple samples. Preferably, two sets of the fixing component 300 and the rotating component 400 are arranged in a one-to-one correspondence along the second direction.

[0076] In this embodiment, reference continues to be made to... Figure 2 As shown, a controller 610 is provided inside the housing 200 to control the operation of the first linear module 310, the second linear module 410 and the rotary drive device 420. The controller 610 can control the rotary drive device 420 to cut and stir the sample in a multi-stage rotation speed manner.

[0077] In this embodiment, reference continues to be made to... Figure 4 As shown, a control screen 620 is provided outside the housing 200. The control screen 620 is a touch screen. Touching the control screen 620 sends control commands to the controller 610 to control the operation of the tissue dissociation device. The connection method and control method between the control screen 620 and the controller 610 are existing technologies and will not be described in detail here.

[0078] In this embodiment, reference continues to be made to... Figure 4 As shown, the housing 200 is provided with a through hole, and the outer side of the fixing seat 320 is provided with a baffle 321. When the fixing seat 320 is in the disengaged position, the baffle 321 can seal the through hole so that the inside of the housing 200 is sealed.

[0079] In this embodiment, reference continues to be made to... Figures 1 to 2 As shown, the first linear module 310 includes a first guide assembly and a first drive assembly. The first guide assembly includes a first slide rail 311 mounted on the bottom of the housing 200 and a first slider 312 slidably connected to the first slide rail 311. The first drive assembly includes a first drive motor 313 mounted on the bottom of the housing 200, a first lead screw 314 driven by the first drive motor 313, and a first threaded sleeve 315 threadedly connected to the first lead screw 314. The first threaded sleeve 315 is fixedly connected to the first slider 312 and to the fixed seat 320. In this embodiment, the first drive motor 313 drives the first lead screw 314 to rotate, thereby causing the first threaded sleeve 315 to move along the first slide rail 311 under the guidance of the first slider 312, achieving the purpose of switching the fixed seat 320 between the disengagement position and the loading position.

[0080] Specifically, a mounting platform 210 is provided at the bottom of the housing 200, and the first slide rail 311 and the first drive motor 313 are both mounted on the mounting platform 210.

[0081] Furthermore, a first bearing seat 211 is provided on the mounting platform 210, and the first lead screw 314 is rotatably connected to the first bearing seat 211 through the bearing.

[0082] It is worth mentioning that a first sensor 3151 is provided on the first threaded sleeve 315, and a first position sensor 212 is provided on the mounting platform 210. When the fixed seat 320 is in the disengaged position, the first sensor 3151 is placed at the first position sensor 212, that is, the first position sensor 212 can detect the first sensor 3151, so that the fixed seat 320 can move accurately to the disengaged position.

[0083] Preferably, the first position sensor 212 is a slotted photoelectric switch.

[0084] In this embodiment, reference continues to be made to... Figures 1 to 2 As shown, the second linear module 410 includes a second guide assembly and a second drive assembly. The second guide assembly includes a second slide rail 411 mounted on the top of the housing 200 and a second slider 412 slidably connected to the second slide rail 411. The second drive assembly includes a second drive motor 413 mounted on the top of the housing 200, a second lead screw 414 driven by the second drive motor 413, and a second threaded sleeve 415 threadedly connected to the second lead screw 414. The second threaded sleeve 415 is fixedly connected to the second slider 412 and to the rotary drive device 420. In this embodiment, the second drive motor 413 drives the second lead screw 414 to rotate, thereby causing the second threaded sleeve 415 to move along the second slide rail 411 under the guidance of the second slider 412, achieving the purpose of connecting and disconnecting the connector 430 from the rotor 130.

[0085] Specifically, the mounting platform 210 is provided with a mounting plate 220 extending in the vertical direction, and the second slide rail 411, the second drive motor 413 and the controller 610 are all mounted on the mounting plate 220.

[0086] Furthermore, a second bearing seat 221 is provided on the mounting plate 220, and the second lead screw 414 is rotatably connected to the second bearing seat 221 through a bearing.

[0087] It is worth mentioning that a second sensor 4151 is provided on the second threaded sleeve 415, and a second position sensor 222 is provided on the mounting plate 220. When the connector 430 is in the initial position, which refers to the position where the connector 430 is disengaged from the rotor 130, the second sensor 4151 is placed at the second position sensor 222, that is, the second position sensor 222 can detect the second sensor 4151, so as to facilitate the control of the movement and positioning of the connector 430.

[0088] Preferably, the second position sensor 222 is a slotted photoelectric switch.

[0089] In this embodiment, reference is made to Figure 3 and Figure 7 As shown, the connector 430 is provided with a connecting groove 510, and the rotor 130 is provided with a connecting shaft 520. The connecting shaft 520 can be inserted into the connecting groove 510 to realize the transmission connection between the connector 430 and the rotor 130.

[0090] Specifically, the connector 430 is provided with a connecting groove 510 at one end facing away from the rotating drive device 420, and the first end of the rotor 130 is provided with a connecting shaft 520 through the through hole of the disintegrating bottle cap 120. Both the connecting groove 510 and the connecting shaft 520 are cross-shaped.

[0091] Specifically, the bottle cap 120 has a perforation at its center, and a sleeve 121 extending into the bottle body 110 is provided at the perforation of the bottle cap 120. A first annular groove 122 is provided inside the sleeve 121. A first annular protrusion 132 is provided on the periphery of the first end of the rotor 130. The first end of the rotor 130 is placed inside the sleeve 121, and the first annular protrusion 132 is slidably placed in the first annular groove 122.

[0092] Furthermore, a first annular limiting plate 133 is provided at the first end of the rotor 130, and a sealing groove 1331 is provided on the periphery of the annular limiting plate. A sealing ring can be installed in the sealing groove 1331 to prevent the sample and digestive fluid from flowing into the space above the first annular limiting plate 133, which would be difficult to clean.

[0093] In this embodiment, reference is made to Figure 8 As shown, a positioning cylinder 322 is provided on the fixing base 320. Multiple positioning protrusions 323 are provided at intervals along the circumferential direction on the inner side of the positioning cylinder 322. Positioning grooves 112 corresponding to the positioning protrusions 323 are provided on the periphery of the detachable bottle body 110. The detachable bottle body 110 can be placed in the positioning cylinder 322 and the positioning protrusions 323 are placed in the positioning grooves 112, which facilitates the fixing of the detachable bottle body 110.

[0094] Preferably, there are two positioning protrusions 323, which are positioned at 180°.

[0095] In this embodiment, a temperature control system (not shown in the figure) is provided inside the housing 200 to ensure that the sample reacts at a suitable temperature. The temperature control system is existing technology and will not be described in detail here.

[0096] Note: For fixing methods not explicitly mentioned in the text, common fixing and connection methods such as threaded connection, welding or bonding can be used as needed.

[0097] Example 2

[0098] The connection method between the connector 430 and the dissociation cap 120 and the rotor 130 in the tissue dissociation device provided in this embodiment differs from that in Embodiment 1. In this embodiment, refer to... Figures 9 to 10 As shown, the connector 430 is provided with a connecting shaft 520, and the rotor 130 is provided with a connecting groove 510. The connecting shaft 520 can be inserted into the connecting groove 510 to realize the transmission connection between the connector 430 and the rotor 130.

[0099] Specifically, the connector 430 is provided with a connecting shaft 520 at one end facing away from the rotating drive device 420, and the first end of the rotor 130 is provided with a connecting groove 510. Both the connecting shaft 520 and the connecting groove 510 are cross-shaped.

[0100] Specifically, a second annular protrusion 123 is provided inside the sleeve 121 of the bottle cap 120, and a second annular groove 134 is provided on the periphery of the first end of the rotor 130. The first end of the rotor 130 is placed inside the sleeve 121, and the second annular protrusion 123 is slidably placed in the second annular groove 134.

[0101] Furthermore, the upper sidewall of the second annular groove 134 is intermittently arranged. Specifically, multiple latches 1341 are spaced apart along the circumference of the rotor 130. The lower end face of the latches 1341 is flat, which together form the upper sidewall of the second annular groove 134. The upper end face of the latches 1341 is inclined to facilitate the disassembly and assembly of the bottle cap 120 and the rotor 130, and to facilitate cleaning.

[0102] Furthermore, a second annular limiting plate 135 is provided at the first end of the rotor 130. There is a gap between the periphery of the annular limiting plate and the inner side of the dissociation bottle cap 120, so that the sample and digestive fluid flowing into the space above the second annular limiting plate 135 can flow back into the dissociation bottle 110.

[0103] Compared with Example 1, the assembly of the dissociated bottle cap 120 and rotor 130 is more convenient.

[0104] The other structures of the tissue dissociation device provided in this embodiment are the same as those in Embodiment 1, and will not be described in detail here.

[0105] Example 3

[0106] The tissue dissociation method using the tissue dissociation apparatus described in Embodiments 1 and 2 includes the following steps:

[0107] S100. Add digestion solution and sample into dissociation bottle 110, and assemble dissociation bottle 110 and dissociation bottle cap 120.

[0108] S200, Move the fixed seat 320 to the feeding position and install the tissue dissociation component 100.

[0109] Specifically, the first drive motor 313 drives the first lead screw 314 to rotate, thereby causing the first threaded sleeve 315 to move outward along the first slide rail 311 under the guidance of the first slider 312, so that the fixed seat 320 is transferred from the disengagement position to the loading position.

[0110] Furthermore, the disassembled bottle 110 is inserted into the positioning cylinder 322 of the fixing base 320, and the positioning protrusion 323 engages with the positioning groove 112 to fix the disassembled bottle 110.

[0111] S300, Move the fixed seat 320 to the release position.

[0112] Specifically, the first drive motor 313 drives the first lead screw 314 to rotate, thereby causing the first threaded sleeve 315 to move along the first slide rail 311 into the housing 200 under the guidance of the first slider 312, so that the fixed seat 320 is transferred from the loading position to the release position.

[0113] S400, the second linear module 410 drives the rotary drive device 420 and the connector 430 to move toward the tissue dissociation assembly 100 so that the connector 430 is connected to the rotor 130 in a transmission connection.

[0114] Specifically, the second drive motor 413 drives the second lead screw 414 to rotate, thereby causing the second threaded sleeve 415 to move along the second slide rail 411 toward the disengaged bottle cap 120 under the guidance of the second slider 412, until the connecting piece 430 is connected to the rotor 130 in a transmission connection.

[0115] S500, the rotary drive device 420 drives the connecting piece 430 to drive the rotor 130 to rotate regularly in both directions, automatically completing the cutting, stirring and digestion of the sample.

[0116] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. Those skilled in the art will be able to make various obvious changes, readjustments, and substitutions without departing from the scope of protection of the present invention. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A tissue dissociation component, characterized in that, include: Dissociation bottle (110), the dissociation bottle (110) is used to hold samples, and the bottom of the dissociation bottle (110) is provided with a first shearing part (111). The bottle cap (120) is detachably connected to the bottle body (110); A rotor (130) has its first end rotatably connected to the disintegrating bottle cap (120), and its second end extends to the bottom of the disintegrating bottle body (110) and is provided with a second shearing part (131); wherein, When the rotor (130) rotates forward, the second shearing section (131) intersects with the first shearing section (111), and the first side of the first shearing section (111) interacts with the second shearing section (131) to cut the sample; When the rotor (130) reverses, the second shearing section (131) intersects with the first shearing section (111), and the second side of the first shearing section (111) interacts with the second shearing section (131) to stir the sample; The first shearing part (111) includes an arc-shaped plate (1111), the first side of the arc-shaped plate (1111) is a stepped blade (1112), and the second side of the arc-shaped plate (1111) is a smooth surface (1113). The second shearing section (131) is paddle-shaped, and a shearing groove (1311) is provided on the lower edge of the second shearing section (131); wherein, When the rotor (130) rotates forward, the stepped blade (1112) of the arc plate (1111) passes through the shearing groove (1311) before the smooth surface (1113) to cut the sample; When the rotor (130) reverses, the smooth surface (1113) of the arc plate (1111) passes through the shear groove (1311) before the stepped blade (1112) to stir the sample; Multiple sets of the arc-shaped plates (1111) are arranged radially along the dissociated bottle body (110), and the arc-shaped plates (1111) in each set are spaced apart circumferentially along the dissociated bottle body (110). The arc-shaped plates (1111) in each group are arranged in an alternating pattern along the radial direction of the dissociated bottle body (110).

2. A tissue dissociation device, characterized in that, include: The tissue dissociation assembly (100) as described in claim 1; Housing (200); The fixing component (300) includes a first linear module (310) disposed within the housing (200) and a fixing seat (320) disposed on the first linear module (310). The first linear module (310) can drive the fixing seat (320) to move along a first direction so that the fixing seat (320) can switch between a disengagement position within the housing (200) and a loading position outside the housing (200). The fixing seat (320) is detachably connected to the disengagement bottle (110). The rotating assembly (400) includes a second linear module (410) disposed within the housing (200), a rotary drive device (420) disposed on the second linear module (410), and a connector (430) connected to the rotary drive device (420). The second linear module (410) can drive the rotary drive device (420) and the connector (430) to move toward the tissue dissociation assembly (100) located at the dissociation position so that the connector (430) is connected to the rotor (130) in a transmission connection. The rotary drive device (420) can drive the rotor (130) to rotate by driving the connector (430) to rotate.

3. The tissue dissociation device according to claim 2, characterized in that, The first linear module (310) includes: The first guide assembly includes a first slide rail (311) mounted on the bottom of the housing (200) and a first slider (312) slidably connected to the first slide rail (311). The first drive assembly includes a first drive motor (313) mounted on the bottom of the housing (200), a first lead screw (314) connected to the first drive motor (313) in transmission, and a first threaded sleeve (315) threadedly connected to the first lead screw (314). The first threaded sleeve (315) is fixedly connected to the first slider (312) and fixedly connected to the fixed seat (320).

4. The tissue dissociation device according to claim 2, characterized in that, The second linear module (410) includes: The second guide assembly includes a second slide rail (411) mounted on the top of the housing (200) and a second slider (412) slidably connected to the second slide rail (411). The second drive assembly includes a second drive motor (413) mounted on the top of the housing (200), a second lead screw (414) connected to the second drive motor (413) in a transmission manner, and a second threaded sleeve (415) threadedly connected to the second lead screw (414). The second threaded sleeve (415) is fixedly connected to the second slider (412) and fixedly connected to the rotary drive device (420).

5. The tissue dissociation device according to claim 2, characterized in that, One of the connector (430) and the rotor (130) is provided with a connecting groove (510), and the other is provided with a connecting shaft (520). The connecting shaft (520) can be inserted into the connecting groove (510) to realize the transmission connection between the connector (430) and the rotor (130).

6. The tissue dissociation device according to claim 2, characterized in that, The fixed base (320) is provided with a positioning cylinder (322). Multiple positioning protrusions (323) are provided circumferentially on the inner side of the positioning cylinder (322). The circumferential side of the dissociated bottle body (110) is provided with positioning grooves (112) that correspond one-to-one with the positioning protrusions (323). The dissociated bottle body (110) can be placed in the positioning cylinder (322) and the positioning protrusions (323) are placed in the positioning grooves (112).

7. The tissue dissociation device according to claim 2, characterized in that, Both the fixed component (300) and the rotating component (400) are provided in multiple sets along the second direction.

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