Dynamic sealing structure and cell processing device

Abstract

The present invention provides a dynamic sealing structure and a cell processing device, wherein the dynamic sealing structure includes a dynamic sealing assembly connected to a guide needle for dynamic sealing, and a shielding assembly used to isolate impurities generated by friction between the guide needle and the dynamic sealing assembly outside the fluid channel of the guide needle; the shielding assembly is sleeved on the guide needle and fixedly connected to the dynamic sealing assembly; a collection space for collecting impurities isolated by the shielding assembly is formed between the shielding assembly and the dynamic sealing assembly. The present invention can isolate impurities generated by friction between the dynamic sealing assembly and the guide needle outside the fluid channel of the guide needle, prevent impurities from entering the centrifugal space of the centrifugal cup, and further ensure that the fluid in the centrifugal space is not contaminated by impurities during the cell processing process. At the same time, the collection space formed between the shielding assembly and the dynamic sealing assembly is used to collect impurities isolated by the shielding assembly, so as to prevent other blockages or interference caused by impurities.

Description

Technical Field

[0001] The invention belongs to the technical field of cell processing, and in particular relates to a dynamic sealing structure and a cell processing device. Background Art

[0002] In an automated cell processing device, the disposable cell processing consumables required mainly include a piping system, a separation cup and a liquid bag. The liquid sample in the liquid bag enters the separation cup through the piping system, and the separation cup rotates at high speed, and uses the density difference to separate the liquid into layers, and then extracts the required liquid layer. In the prior art, when the centrifugal cup rotates at high speed, the liquid needs to be sealed by a dynamic sealing structure, and the friction between the guide needle and the pan-plug in the dynamic sealing structure will produce impurities (such as insoluble particles), which will cause the generated impurities to flow into the separation cup with the liquid and contaminate the liquid sample. Summary of the invention

[0003] The present invention aims to solve the technical problems in the prior art that the dynamic sealing structure in the cell processing device is prone to friction to generate impurities and cause sample contamination, and provides a dynamic sealing structure and a cell processing device.

[0004] In view of the above technical problems, an embodiment of the present invention provides a dynamic sealing structure, comprising a dynamic sealing assembly dynamically connected to a guide needle and a shielding assembly for isolating impurities generated by friction between the guide needle and the dynamic sealing assembly outside a fluid channel of the guide needle;

[0005] The shielding assembly is sleeved on the guide needle and fixedly connected to the dynamic sealing assembly; a collecting space for collecting impurities isolated by the shielding assembly is formed between the shielding assembly and the dynamic sealing assembly.

[0006] Optionally, a blocking gap is provided between the shielding assembly and the guide needle for blocking the liquid in the fluid channel of the guide needle from entering the collection space.

[0007] Optionally, a first barrier layer is provided on the shielding component; a second barrier layer is provided on the guide needle; the barrier gap is formed by the relative spacing of the first barrier layer and the second barrier layer; the first barrier layer and / or the second barrier layer are both hydrophilic layers.

[0008] Optionally, a first barrier layer is provided on the shielding component; a second barrier layer is provided on the guide needle; the barrier gap is formed by the relative spacing of the first barrier layer and the second barrier layer; and both the first barrier layer and the second barrier layer are hydrophobic layers.

[0009] Optionally, the guide needle includes a first conduit; the fluid channel includes a first fluid channel arranged in the first conduit; the dynamic sealing assembly includes a first pan plug sleeved on the first conduit; the shielding assembly includes a first gasket sleeved on the first conduit and fixedly connected to the first pan plug; the first gasket is used to isolate impurities generated by friction between the first conduit and one end of the first pan plug close to the first gasket from outside the first fluid channel;

[0010] The collecting space comprises a first collecting space formed between the first gasket and the first pan-seal; the first collecting space is used to collect impurities isolated by the first gasket;

[0011] The blocking gap includes a first gap disposed between the first gasket and the first conduit, and the first gap is used to block the liquid in the first fluid channel from entering the first collecting space.

[0012] Optionally, a first collecting groove having a first opening is provided on the first gasket, the first opening is communicated with the first collecting space, and the first collecting groove is used to store impurities entering from the first collecting space through the first opening.

[0013] Optionally, the guide needle further includes a second conduit sleeved outside the first conduit, and the fluid channel further includes a second fluid channel arranged between the first conduit and the second conduit; the shielding assembly further includes a second gasket sleeved on the first conduit and the second conduit, and the first gasket and the second gasket are respectively arranged on opposite sides of the first pan-plug; the second gasket is used to isolate impurities generated by friction between the first conduit and an end of the first pan-plug close to the second gasket outside the second fluid channel;

[0014] The second gasket includes a first sealing portion, and the collecting space also includes a second collecting space formed between the first sealing portion and the first pan-seal; the second collecting space is used to collect impurities isolated by the second gasket;

[0015] The barrier gap further includes a second gap disposed between the first sealing portion and the first conduit, and the second gap is used to block the liquid in the second fluid channel from entering the second collecting space.

[0016] Optionally, a second collecting groove having a second opening is provided on the first sealing portion, the second opening is communicated with the second collecting space, and the second collecting groove is used to store impurities entering from the second collecting space through the second opening.

[0017] Optionally, the dynamic seal assembly further comprises a second Varistor sleeved on the second conduit; the second gasket is fixedly connected to the first Varistor and / or the second Varistor; the second gasket is further used to isolate impurities generated by friction between the second conduit and the second Varistor from outside the second fluid channel;

[0018] The second gasket further includes a second sealing portion, and the collection space further includes a third collection space formed between the second sealing portion and the second pan-seal; the third collection space is used to collect impurities isolated by the second gasket;

[0019] The barrier gap further includes a third gap disposed between the second sealing portion and the second conduit, and the third gap is used to block the liquid in the second fluid channel from entering the third collecting space.

[0020] Optionally, a third collecting groove having a third opening is provided on the second sealing portion, the third opening is communicated with the third collecting space, and the third collecting groove is used to store impurities entering from the third collecting space through the third opening.

[0021] The present invention also provides a cell processing device, comprising a guide needle, a centrifugal cup and the above-mentioned dynamic sealing structure; the guide needle is inserted into the centrifugal cup, and the fluid channel of the guide needle is connected to the centrifugal space of the centrifugal cup; the shielding assembly and the dynamic sealing assembly are both fixedly mounted on the centrifugal cup.

[0022] In the present invention, the dynamic sealing structure includes a dynamic sealing component connected to the guide needle in a dynamic seal and a shielding component used to isolate impurities generated by friction between the guide needle and the dynamic sealing component outside the fluid channel of the guide needle; the shielding component is sleeved on the guide needle and fixedly connected to the dynamic sealing component; a collection space for collecting impurities isolated by the shielding component is formed between the shielding component and the dynamic sealing component. The dynamic sealing component of the present invention is connected to the guide needle in a dynamic seal. During the cell treatment process, the centrifugal cup rotates at a high speed, and the dynamic sealing component rotates with the centrifugal cup. Impurities generated by friction between the dynamic sealing component and the guide needle (such as insoluble particles generated by friction between the dynamic sealing component and the guide needle) will be isolated by the shielding component sleeved on the guide needle outside the fluid channel of the guide needle. In this way, impurities can be prevented from entering the centrifugal space of the centrifugal cup, thereby ensuring that the fluid in the centrifugal space is not contaminated by impurities during the cell treatment process. At the same time, a collection space is formed between the shielding component and the dynamic sealing component, in which impurities isolated by the shielding component can be collected to prevent other blockages or interference caused by impurities. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0024] Figure 1 It is a structural schematic diagram of a dynamic sealing structure and a guide needle provided in one embodiment of the present invention.

[0025] Figure 2 It is a partial structural schematic diagram of a cell processing device provided in one embodiment of the present invention.

[0026] The reference numerals in the specification are as follows:

[0027] 1. Dynamic sealing structure; 10. Guide needle; 110. First conduit; 120. Second conduit; 20. Fluid channel; 210. First fluid channel; 220. Second fluid channel; 30. Dynamic sealing assembly; 310. First flood plug; 320. Second flood plug; 40. Shielding assembly; 410. First gasket; 411. First collecting tank; 420. Second gasket; 421. First sealing part; 4211. Second collecting tank; 422. Second sealing part; 4221. Third collecting tank; 423. Gasket body; 50. Collecting space; 510. First collecting space; 520. Second collecting space; 530. Third collecting space; 60. Barrier gap; 610. First gap; 620. Second gap; 630. Third gap; 2. Centrifugal cup; 21. Centrifugal space. DETAILED DESCRIPTION

[0028] In order to make the technical problems, technical solutions and beneficial effects solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0029] It should be understood that the directions or positional relationships indicated by terms such as "upper", "lower", "left", "right", "front", "back", and "middle" are based on the directions or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the components or elements referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation of the present invention.

[0030] like Figure 1 and Figure 2As shown, an embodiment of the present invention provides a dynamic sealing structure 1, comprising a dynamic sealing assembly 30 dynamically connected to a guide needle 10 and a shielding assembly 40 for isolating impurities generated by friction between the guide needle 10 and the dynamic sealing assembly 30 outside the fluid channel 20 of the guide needle 10; the shielding assembly 40 is sleeved on the guide needle 10 and fixedly connected to the dynamic sealing assembly 30; a collecting space 50 for collecting impurities isolated by the shielding assembly 40 is formed between the shielding assembly 40 and the dynamic sealing assembly 30. The guide needle 10 is inserted into the centrifugal cup 2 of the cell processing device, and the fluid channel 20 of the guide needle 10 is connected to the centrifugal space 21 of the centrifugal cup 2; and then the fluid (such as a liquid sample) is injected into the centrifugal space 21 through the above-mentioned fluid channel 20, or the fluid (including gas or liquid) in the centrifugal space 21 is extracted from the centrifugal space 21. The shielding component 40 and the dynamic sealing component 30 are both fixedly mounted on the centrifugal cup 2, and the dynamic sealing component 30 is dynamically sealedly connected to the guide needle 10, so as to ensure that the centrifugal cup 2 can rotate at a high speed relative to the guide needle 10 while preventing the liquid in the centrifugal space 21 from flowing out from the connection position between the guide needle 10 and the centrifugal cup 2; the shielding component 40 can isolate the impurities generated by the friction between the guide needle 10 and the dynamic sealing component 30 outside the fluid channel 20 of the guide needle 10, and at the same time, a collecting space 50 is formed between the shielding component 40 and the dynamic sealing component 30, in which the impurities isolated by the shielding component 40 can be collected to avoid other blockages or interference caused by the impurities.

[0031] The dynamic seal assembly 30 of the dynamic seal structure 1 of the present invention is dynamically sealed to the guide needle 10. During the cell treatment process, the centrifugal cup 2 rotates at high speed, and the dynamic seal assembly 30 rotates together with the centrifugal cup 2. The impurities generated by the friction between the dynamic seal assembly 30 and the guide needle 10 (such as insoluble particles generated by the friction between the dynamic seal assembly 30 and the guide needle 10) will be isolated by the shielding assembly 40 sleeved on the guide needle 10 outside the fluid channel 20 of the guide needle 10. In this way, impurities can be prevented from entering the centrifugal space 21 of the centrifugal cup 2, thereby ensuring that the fluid in the centrifugal space 21 is not contaminated by impurities during the cell treatment process. At the same time, a collection space 50 is formed between the shielding assembly 40 and the dynamic seal assembly 30, in which the impurities isolated by the shielding assembly 40 can be collected to prevent other blockages or interference caused by impurities.

[0032] In one embodiment, if Figure 1As shown, a barrier gap 60 is provided between the shielding assembly 40 and the guide needle 10 for preventing the liquid in the fluid channel 20 of the guide needle 10 from entering the collection space 50. It can be understood that the barrier gap 60 is provided between the shielding assembly 40 and the guide needle 10 (such as the first gap 610 formed between the first gasket 410 and the guide needle 10 mentioned later, and the second gap 620 and the third gap 630 formed between the second gasket 420 and the guide needle 10). Preferably, the shielding assembly 40 is provided with a first barrier layer; the guide needle 10 is provided with a second barrier layer; and the barrier gap 60 is formed by the relative spacing of the first barrier layer and the second barrier layer.

[0033] In one aspect of this embodiment, the first barrier layer and / or the second barrier layer are both hydrophilic layers; the gap size of the barrier gap ranges from 0.1mm to 0.15mm. It is understandable that only the first barrier layer and / or the second barrier layer can be set as a hydrophilic layer made of a hydrophilic material, while the shielding component 40 and other parts of the guide needle 10 are made of other materials. The entire shielding component 40 (including the first gasket 410 and the second gasket 420 mentioned later) can also be made of a hydrophilic material. In this case, the first barrier layer corresponding to the shielding component 40 must be a hydrophilic layer; or the entire guide needle 10 can be made of a hydrophilic material. In this case, the second barrier layer corresponding to the guide needle 10 must be a hydrophilic layer; it is understandable that the first barrier layer and the second barrier layer can all be hydrophilic layers, or only one of them can be a hydrophilic layer, but when one of them is a hydrophilic layer, as long as the corresponding gap The gap size ranges from 0.1mm to 0.15mm. When the centrifugal cup rotates, the shielding component 40 and the guide needle 10 will not contact each other, and an ultra-low flow rate area of ​​the fluid will be formed in the barrier gap 60, so that there will be no liquid exchange between the fluid channel 20 of the guide needle 10 and the collection space 50. In this way, impurities generated by the friction between the dynamic sealing component 30 and the guide needle 10 will be isolated and collected in the collection space 50, and cannot flow out of the collection space 50 with the flow of the liquid, nor can they flow into the fluid channel of the guide needle 10 from the barrier gap 60.

[0034] In another aspect of the present embodiment, the first barrier layer and the second barrier layer are both hydrophobic layers; the gap size of the barrier gap ranges from 0.1 mm to 0.5 mm; that is, the inner side wall of the barrier gap 60 is covered with a hydrophobic layer. It can be understood that the hydrophobic layer is arranged on the inner side wall of the shielding component 40 opposite to the guide needle 10. Specifically, in one embodiment, a hydrophobic surface treatment can be performed on the side wall opposite to the shielding component 40 and the guide needle 10, such as coating a layer of ceramic film. In this case, the ceramic film coated on the side wall position opposite to the shielding component 40 and the guide needle 10 is the first barrier layer and the second barrier layer; in another embodiment, the entire shielding component 40 (including the first gasket 410 and the second gasket 420 mentioned later) and the guide needle 10 can also be made of hydrophobic material. In this case, the first barrier layer on the shielding component 40 and the second barrier layer on the guide needle 10 must also be hydrophobic layers. By setting the first barrier layer and the second barrier layer as hydrophobic layers, and setting the gap size of the barrier gap to be in the range of 0.1mm-0.5mm, the shielding component 40 and the guide needle 10 can directly block the liquid outside the collection space 50 through capillary action, so that there is no liquid in the collection space 50, and the impurities collected in the collection space 50 do not have the conditions to flow out of the collection space 50 to the fluid channel 20 of the guide needle 10. Further, the hydrophobic material may include but is not limited to PTFE (polytetrafluoroethylene), PE (polyethylene), PP (polypropylene) and other hydrophobic materials. It is understandable that in the present invention, only the first barrier layer and the second barrier layer on the side wall opposite to the guide needle 10 of the shielding component 40 can be set as a hydrophobic material layer (made of PTFE, PE, PP), and it is not necessary to be made of hydrophobic material as a whole, as long as the effect of blocking the liquid outside the collection space 50 can be achieved.

[0035] It can be seen from the above that when the barrier gap size ranges from 0.1 mm to 0.15 mm, the first barrier layer and the second barrier layer are configured as a hydrophobic layer or a hydrophilic layer, which can block the liquid outside the collection space 50 .

[0036] In one embodiment, if Figure 1As shown, the guide needle 10 includes a first conduit 110; the fluid channel 20 includes a first fluid channel 210 disposed in the first conduit 110; the dynamic seal assembly 30 includes a first pan plug 310 sleeved on the first conduit 110; the shielding assembly 40 includes a first gasket 410 sleeved on the first conduit 110 and fixedly connected to the first pan plug 310; the first gasket 410 is used to isolate impurities generated by friction between the first conduit 110 and one end of the first pan plug 310 close to the first gasket 410 outside the first fluid channel 210. The collection space 50 includes a first collection space 510 formed between the first gasket 410 and the first pan plug 310; the first collection space 510 is used to collect impurities isolated by the first gasket 410; the blocking gap 60 includes a first gap 610 disposed between the first gasket 410 and the first conduit 110, and the first gap 610 is used to block the liquid in the first fluid channel 210 from entering the first collection space. It can be understood that the first conduit 110 is in communication with the centrifugal space 21 to input the fluid in the first fluid channel 210 into the centrifugal space 21, or to extract the fluid (including gas or liquid) in the centrifugal space 21 from the centrifugal space 21. Figure 1 As shown, in this embodiment, the first collecting space 510 formed between the first gasket 410 and the first pan plug 310 is used to collect impurities (insoluble particles) generated by high-speed friction between the guide needle 10 and the bottom surface of the first pan plug 310. The first gap 610 is used to prevent liquid exchange between the first fluid channel 210 in the first conduit 110 and the first collecting space 510, thereby preventing the liquid in the first fluid channel 210 from entering the first collecting space 510.

[0037] Furthermore, if Figure 1 As shown, a first collecting groove 411 having a first opening is provided on the first gasket 410, and the first opening is communicated with the first collecting space 510, and the first collecting groove 411 is used to store impurities entering from the first collecting space 510 through the first opening. It is understandable that the first collecting groove 411 is arranged on the surface of the first gasket 410 opposite to the first flood plug 310, on the side away from the guide needle 10, so that when the shielding assembly 40 rotates at a high speed with the separation cup, the impurities generated by the high-speed friction between the guide needle 10 and the bottom surface of the first flood plug 310 appear in the first collecting space 510, and will be thrown into the first collecting groove 411 by centrifugal force to avoid the accumulation of impurities at the position of the first gap 610, thereby ensuring that the first gap 610 will not be filled with impurities and affect its function. Among them, the first collecting groove 411 is concavely formed on the surface of the first gasket 410 opposite to the first flood plug 310, and its specific shape can be set according to needs, and is not limited to Figure 1 The shapes shown in .

[0038] In one embodiment, if Figure 1 As shown, the guide needle 10 further includes a second conduit 120 sleeved outside the first conduit 110, and the fluid channel 20 further includes a second fluid channel 220 arranged between the first conduit 110 and the second conduit 120; the shielding assembly 40 further includes a second gasket 420 sleeved on the first conduit 110 and the second conduit 120, and the first gasket 410 and the second gasket 420 are respectively arranged on opposite sides of the first pan plug 310; the second gasket 420 is used to place the first conduit 110 and the first pan plug 310 close to one end of the second gasket 420 The impurities generated by the friction between the first and second fluid channels 220 are isolated outside the second fluid channel 220; the second gasket 420 includes a first sealing portion 421, and the collection space 50 also includes a second collection space 520 formed between the first sealing portion 421 and the first pan plug 310; the second collection space 520 is used to collect the impurities isolated by the second gasket 420; the blocking gap 60 also includes a second gap 620 arranged between the first sealing portion 421 and the first conduit 110, and the second gap 620 is used to prevent the liquid in the second fluid channel 220 from entering the second collection space 520. Further, the second gasket 420 also includes a gasket body 423 connected between the first sealing portion 421 and the second sealing portion 422, and the gasket body 423 is provided with a liquid outlet (not shown) communicating with the second fluid channel 220. It can be understood that the first conduit 110 is in communication with the centrifugal space 21 to input the fluid in the first fluid channel 210 into the centrifugal space 21, or to extract the fluid (including gas or liquid) in the centrifugal space 21 from the centrifugal space 21. The second conduit 120 is connected to the centrifugal space 21 through the liquid outlet provided on the gasket body 423, thereby inputting the fluid in the second fluid channel 220 into the centrifugal space 21. Figure 1 As shown, in this embodiment, the second collecting space 520 formed between the first sealing portion 421 of the second gasket 420 and the first pan plug 310 is used to collect impurities (insoluble particles) generated by high-speed friction between the guide needle 10 and the top surface of the first pan plug 310. The second gap 620 is used to prevent liquid exchange between the second fluid channel 220 and the second collecting space 520, thereby preventing the liquid in the second fluid channel 220 in the guide needle 10 from entering the second collecting space 520.

[0039] In one embodiment, if Figure 1As shown, a second collecting groove 4211 having a second opening is provided on the first sealing portion 421, and the second opening is connected to the second collecting space 520, and the second collecting groove 4211 is used to store impurities entering from the second collecting space 520 through the second opening. It is understandable that the second collecting groove 4211 is arranged on the lower surface of the first sealing portion 421 of the second gasket 420 (the surface of the first sealing portion 421 opposite to the first flood plug 310) on the side relatively away from the guide needle 10, so that when the shielding assembly 40 rotates at a high speed with the separation cup, the impurities generated by the high-speed friction between the guide needle 10 and the top surface of the first flood plug 310 appear in the second collecting space 520, and will be thrown into the second collecting groove 4211 by centrifugal force to avoid the accumulation of impurities at the position of the second gap 620, thereby ensuring that the second gap 620 will not be filled with impurities and affect its function. It is understandable that in Figure 1 In the embodiment shown, the second collecting groove 4211 is an inclined groove arranged on the first sealing portion 421 of the second gasket 420. In this way, when the shielding assembly 40 rotates at high speed with the separation cup, the impurities generated by the high-speed friction between the guide needle 10 and the top surface of the first flood plug 310 will be thrown by centrifugal force into the edge gap away from the guide needle 10 in the second collecting space 520 formed between the inclined groove and the first flood plug 310, so as to avoid the impurities from accumulating at the position of the second gap 620. In this embodiment, the form of the second collecting groove 4211 is not limited to Figure 1 As shown in , it can be set according to requirements as long as the effect of collecting impurities to prevent impurities from accumulating at the position of the second gap 620 can be achieved.

[0040] In one embodiment, if Figure 1 As shown, the dynamic seal assembly 30 further includes a second varistor 320 sleeved on the second conduit 120; the second gasket 420 is fixedly connected to the first varistor 310 and / or the second varistor 320; the second gasket 420 is also used to isolate impurities generated by friction between the second conduit 120 and the second varistor 320 outside the second fluid channel 220; the second gasket 420 further includes a second sealing portion 422, and the collection space 50 further includes a third collection space 530 formed between the second sealing portion 422 and the second varistor 320; the third collection space 530 is used to collect impurities isolated by the second gasket 420; the blocking gap 60 further includes a third gap 630 provided between the second sealing portion 422 and the second conduit 120, and the third gap 630 is used to prevent the liquid in the second fluid channel 220 from entering the third collection space 530. It can be understood that, as Figure 1As shown, in this embodiment, the third collecting space 530 formed between the second sealing portion 422 of the second gasket 420 and the second pan plug 320 is used to collect impurities (insoluble particles) generated by high-speed friction between the guide needle 10 and the bottom surface of the second pan plug 320. The third gap 630 is used to prevent liquid exchange between the second fluid channel 220 and the third collecting space 530, thereby preventing the liquid in the second fluid channel 220 in the guide needle 10 from entering the third collecting space 530.

[0041] In one embodiment, if Figure 1 As shown, the second sealing part 422 is provided with a third collecting groove 4221 having a third opening, and the third opening is connected to the third collecting space 530, and the third collecting groove 4221 is used to store impurities entering from the third collecting space 530 through the third opening. It can be understood that the third collecting groove 4221 is arranged on the upper surface of the second sealing part 422 of the second gasket 420 (the surface of the second sealing part 422 opposite to the second flood plug 320) on the side away from the guide needle 10, so that when the shielding assembly 40 rotates at a high speed with the separation cup, the impurities generated by the high-speed friction between the guide needle 10 and the bottom surface of the second flood plug 320 appear in the third collecting space 530, and will be thrown into the third collecting groove 4221 by centrifugal force to avoid the accumulation of impurities at the position of the third gap 630, thereby ensuring that the third gap 630 will not be filled with impurities and affect its function. Among them, the third collecting groove 4221 is concavely formed on the surface of the second sealing part 422 opposite to the second flood plug 320, and its specific shape can be set according to needs, and is not limited to Figure 1 The shapes shown in .

[0042] The present invention also provides a cell processing device, such as Figure 1 and Figure 2 The invention comprises a guide needle 10, a centrifugal cup 2 and the above-mentioned dynamic sealing structure 1; the guide needle 10 is inserted into the centrifugal cup 2, and the fluid channel 20 of the guide needle 10 is connected with the centrifugal space 21 of the centrifugal cup 2; the shielding component 40 and the dynamic sealing component 30 are both fixedly mounted on the centrifugal cup 2. That is, the shielding component 40 and the dynamic sealing component 30 are fixedly connected by being fixedly mounted on the centrifugal cup 2. When the centrifugal cup 2 rotates, the shielding component 40 and the dynamic sealing component 30 rotate relative to the guide needle 10.

[0043] In the cell processing device of the present invention, the dynamic sealing assembly 30 of the dynamic sealing structure 1 is dynamically sealed to the guide needle 10. During the cell processing process, the centrifugal cup 2 rotates at high speed, and the dynamic sealing assembly 30 rotates together with the centrifugal cup 2. The impurities generated by the friction between the dynamic sealing assembly 30 and the guide needle 10 (such as insoluble particles generated by the friction between the dynamic sealing assembly 30 and the guide needle 10) will be isolated by the shielding assembly 40 sleeved on the guide needle 10 outside the fluid channel 20 of the guide needle 10. In this way, impurities can be prevented from entering the centrifugal space 21 of the centrifugal cup 2, thereby ensuring that the fluid in the centrifugal space 21 is not contaminated by impurities during the cell processing process. At the same time, a collection space 50 is formed between the shielding assembly 40 and the dynamic sealing assembly 30, in which the impurities isolated by the shielding assembly 40 can be collected to prevent other blockages or interference caused by impurities.

[0044] For the detailed description of each component in the above cell processing device, reference may be made to the description of the dynamic sealing structure 1 in the above embodiment, which will not be repeated here.

[0045] The above are merely embodiments of the dynamic sealing structure 1 and the cell processing device of the present invention, and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A dynamic sealing structure, characterized in that: It includes a dynamic sealing assembly connected to the guide needle in a dynamic seal and a shielding assembly used to isolate impurities generated by friction between the guide needle and the dynamic sealing assembly outside the fluid channel of the guide needle; The shielding assembly is sleeved on the guide needle and fixedly connected to the dynamic sealing assembly; a collecting space for collecting impurities isolated by the shielding assembly is formed between the shielding assembly and the dynamic sealing assembly; A barrier gap is provided between the shielding assembly and the guide needle for preventing the liquid in the fluid channel of the guide needle from entering the collection space; The shielding component is provided with a first barrier layer; the guide needle is provided with a second barrier layer; the barrier gap is formed by the first barrier layer and the second barrier layer being arranged relative to each other; The guide needle includes a first catheter; the fluid channel includes a first fluid channel arranged in the first catheter; the dynamic sealing assembly includes a first pan-plug sleeved on the first catheter; the shielding assembly includes a first gasket sleeved on the first catheter and fixedly connected to the first pan-plug; the collecting space includes a first collecting space formed between the first gasket and the first pan-plug; the blocking gap includes a first gap arranged between the first gasket and the first catheter.

2. The dynamic sealing structure according to claim 1, characterized in that: The first barrier layer and / or the second barrier layer are both hydrophilic layers.

3. The dynamic sealing structure according to claim 1, characterized in that: The first barrier layer and the second barrier layer are both hydrophobic layers.

4. The dynamic sealing structure according to claim 1, characterized in that: The first gasket is provided with a first collecting groove having a first opening, the first opening is communicated with the first collecting space, and the first collecting groove is used to store impurities entering from the first collecting space through the first opening.

5. The dynamic sealing structure according to claim 1, characterized in that: The guide needle further includes a second conduit sleeved outside the first conduit, and the fluid channel further includes a second fluid channel arranged between the first conduit and the second conduit; the shielding assembly further includes a second gasket sleeved on the first conduit and the second conduit, and the first gasket and the second gasket are respectively arranged on opposite sides of the first pan-plug; the second gasket is used to isolate impurities generated by friction between the first conduit and an end of the first pan-plug close to the second gasket from the second fluid channel; The second gasket includes a first sealing portion, and the collecting space also includes a second collecting space formed between the first sealing portion and the first pan-seal; the second collecting space is used to collect impurities isolated by the second gasket; The barrier gap further includes a second gap disposed between the first sealing portion and the first conduit, and the second gap is used to block the liquid in the second fluid channel from entering the second collecting space.

6. The dynamic sealing structure according to claim 5, characterized in that: A second collecting groove having a second opening is provided on the first sealing portion. The second opening is communicated with the second collecting space. The second collecting groove is used to store impurities entering from the second collecting space through the second opening.

7. The dynamic sealing structure according to claim 5, characterized in that: The dynamic seal assembly further comprises a second variable-plug sleeved on the second conduit; the second gasket is fixedly connected to the first variable-plug and / or the second variable-plug; the second gasket is also used to isolate impurities generated by friction between the second conduit and the second variable-plug outside the second fluid channel; The second gasket further includes a second sealing portion, and the collection space further includes a third collection space formed between the second sealing portion and the second pan-seal; the third collection space is used to collect impurities isolated by the second gasket; The barrier gap further includes a third gap disposed between the second sealing portion and the second conduit, and the third gap is used to block the liquid in the second fluid channel from entering the third collecting space.

8. The dynamic sealing structure according to claim 7, characterized in that: The second sealing portion is provided with a third collecting groove having a third opening, the third opening is communicated with the third collecting space, and the third collecting groove is used to store impurities entering from the third collecting space through the third opening.

9. A cell processing device, characterized in that: It comprises a guide needle, a centrifugal cup and a dynamic sealing structure as described in any one of claims 1 to 8; the guide needle is inserted into the centrifugal cup, and the fluid channel of the guide needle is connected to the centrifugal space of the centrifugal cup; the shielding component and the dynamic sealing component are both fixedly mounted on the centrifugal cup.

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