A cell centrifuge for Chinese mitten crab experiments

Abstract

The invention provides a cell centrifuge for Chinese mitten crab experiments, comprising a machine base, a sealing cover that can be flipped open is arranged on the top of the machine base, a central rotating shaft that is connected to a high-speed motor in the machine base is arranged in the center of the machine base, a centrifugal drum that is driven to rotate by the central rotating shaft is fixedly sleeved on the outer side of the central rotating shaft, a test tube rack on the centrifugal drum is hinged to the centrifugal drum, and the bottom is movably connected to the central rotating shaft by a telescopic piston rod, and the hinged movable arrangement of the test tube rack and the piston rod at the bottom can change the overall angle of the test tube rack by changing the bottom position of the test tube rack under centrifugal action, and during centrifugal movement, the test tube rack drives the internal test tube to tilt under the action of centrifugal force, so as to maximize the centrifugal action, and falls back under the action of gravity when stopping, and is vertically arranged, so as to be convenient to take, reduce the angle change during taking, and reduce the degree of re-mixing and dissolving of the separated medium, and has good development prospects.

Description

Technical Field

[0001] The invention relates to the field of cell centrifuges, in particular to a cell centrifuge used for Chinese mitten crab experiments. Background Art

[0002] A centrifuge is a machine that uses centrifugal force to separate liquids from solid particles, or to separate the components of a mixture of liquids. Centrifuges are mainly used to separate solid particles from liquids in suspensions, or to separate two immiscible liquids of different densities in emulsions. They are often used in various medical tests and biological research projects.

[0003] In order to separate different media from each other, a stronger centrifugal force needs to act on the media. The installation of test tubes in the centrifuge usually adopts the configuration of tilted test tubes, and uses the increase of the force arm brought by the length of the test tubes themselves to increase the centrifugal force at the tail end of the test tubes, thereby accelerating the separation of the media. For example, a fast centrifuge for stem cells that prevents centrifugal contamination with application number CN202011001787.5 adopts a tilted test tube solution. However, this also brings inconvenience to the taking of test tubes. Compared with vertically set test tubes, the taking of tilted test tubes must maintain a certain extraction angle, which is more likely to cause bumps and damage to the test tubes and experimental samples. The shaking caused by the angle change during the process will also cause the medium to dissolve again to a certain extent, resulting in a certain measurement error in the results, which is not conducive to the accuracy of experimental detection.

[0004] Therefore, a new type of cell centrifuge is needed to solve the above problems. Summary of the invention

[0005] The purpose of the present invention is to provide a cell centrifuge for Chinese mitten crab experiments.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A cell centrifuge for Chinese mitten crab experiments comprises a machine base, a sealing cover that can be flipped open is arranged on the top of the machine base, a central rotating shaft that is connected to a high-speed motor in the machine base is arranged in the center of the machine base, a centrifugal drum that is driven to rotate by the central rotating shaft is fixedly sleeved on the outer side of the central rotating shaft, a test tube rack is arranged on the centrifugal drum to fix experimental samples, and is characterized in that the top of the test tube rack is hinged to the centrifugal drum, and the bottom is movably connected to the central rotating shaft through a telescopic piston rod.

[0008] As a further solution of the present invention: a groove is concavely provided on the front side of the machine base, an operation panel for controlling the movement of the high-speed motor is embedded in the groove, and when the sealing cover is closed, the space above the groove is shielded.

[0009] As a further solution of the present invention: a capsule-shaped groove perpendicular to the central rotating shaft is formed on the surface of the centrifugal drum, and a test tube rack is arranged in the groove structure at one end close to the central rotating shaft.

[0010] As a further solution of the present invention: the test tube rack includes a collar, a base and a connecting arm. The collar is an annular structure, and horizontal connecting arms are symmetrically arranged on both sides for hinged connection with the centrifugal drum. The lower side of the collar is connected to a hemispherical base, and a connecting arm is vertically arranged at the bottom of the base for hinged connection with the piston rod.

[0011] As a further solution of the present invention: the central rotating shaft includes a shaft body and a piston rod. The shaft body is a hollow shaft body, and a accommodating chamber is arranged inside. The accommodating chamber is filled with hydraulic oil medium. An ear plate structure is circumferentially arranged on the outer side of the shaft body, and the piston rod is hinged between the ear plates. The front end of the piston rod is hinged to the bottom of the test tube rack, and the oil chamber in the piston rod is connected to the accommodating chamber in the shaft body through a guide hose.

[0012] As a further solution of the present invention: the accommodating cavity as a whole is a shuttle-shaped structure with a large diameter at the connection with the diversion hose and small diameters at the upper and lower ends.

[0013] As a further solution of the present invention: a guide block structure protruding toward the center of the shaft body is provided on the inner wall of the accommodating cavity, between adjacent guide hoses.

[0014] As a further solution of the present invention: the bottom of the accommodating chamber is connected to a vertically arranged channel, the channel is also filled with hydraulic oil medium, a pressure regulating piston is slidably arranged at the bottom of the channel, and a pressure relief hole connected to the external atmospheric environment is penetrated on the side wall of the shaft body on the lower side of the pressure regulating piston.

[0015] As a further solution of the present invention: a protruding limiting structure is arranged on the outer side of the pressure regulating piston to cooperate with the shaft structure to limit the stroke of the pressure regulating piston. Beneficial Effects

[0016] 1. The test tube rack on the centrifugal drum of the present invention is hinged to the centrifugal drum, and the bottom is movably connected to the central rotating shaft through a telescopic piston rod. By means of the hinged movable arrangement of the test tube rack and the piston rod at the bottom, the overall angle of the test tube rack can be changed by changing the bottom position of the test tube rack under the centrifugal action. During centrifugal movement, the test tube rack drives the internal test tube to tilt under the action of centrifugal force, so as to maximize the centrifugal action. When it stops, it falls back under the action of gravity. The vertical arrangement is convenient for taking, and also reduces the angle change during taking, thereby reducing the degree of re-mixing and dissolving of the separated medium.

[0017] 2. The central rotating shaft of the present invention comprises a shaft body and a piston rod. The shaft body is a hollow shaft body, and a receiving chamber is arranged inside. The receiving chamber is filled with hydraulic oil medium. An ear plate structure is arranged circumferentially on the outer side of the shaft body, and a piston rod is hinged between the ear plates. The front end of the piston rod is hinged to the bottom of the test tube rack. The oil chamber in the piston rod is connected with the receiving chamber in the shaft body through a guide hose. Through the arrangement of the receiving chamber and the piston rod outside the receiving chamber, when the central rotating shaft rotates, the test tube rack moves toward a horizontal angle under the action of centrifugal force, and the angle change of the test tube rack drives the piston rod to expand. The hydraulic oil medium in the receiving chamber flows into the piston rod under the action of negative pressure. By limiting the maximum flow rate of the guide hose, the expansion of the piston rod can be hindered, that is, the angle change speed of the test tube rack is controlled to prevent vibration caused by excessive angle change. After the centrifugation is completed, the falling speed of the test tube rack can also be controlled. Finally, the test tube rack and the test tube fall back to a vertical state under the action of gravity, which is convenient for picking up and operating.

[0018] 3. The bottom of the accommodating chamber of the present invention is connected to a vertically arranged channel, and the channel is also filled with hydraulic oil medium. A pressure regulating piston is slidably arranged at the bottom of the channel. Through the arrangement of the channel and the pressure regulating piston, when the hydraulic oil medium in the accommodating chamber enters the piston rod under the action of centrifugal force, the oil pressure in the accommodating chamber decreases, which will drive the pressure regulating piston to rise, and press the hydraulic oil medium in the channel into the accommodating chamber, thereby balancing the oil pressure in the accommodating chamber and preventing the oil pressure from causing excessive obstruction to the deployment of the piston rod.

[0019] 4. The outer side of the pressure regulating piston of the present invention is provided with a protruding limiting structure which cooperates with the shaft structure to limit the stroke of the pressure regulating piston to prevent over-stroke movement. The change range of the hydraulic pressure in the accommodating chamber and the piston rod can be controlled by limiting the stroke of the pressure regulating piston, and the stroke of the piston rod can be flexibly controlled through hydraulic pressure. Compared with physically limiting the stroke of the piston rod, the recoil force when moving to the stroke limit position is reduced, thereby improving the stability of the device during operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0021] Figure 2 For the present invention Figure 1 A schematic diagram of the enlarged structure.

[0022] Figure 3 It is a schematic diagram of the top cover of the present invention in the lowered state.

[0023] Figure 4 It is a schematic diagram of the connection between the test tube rack and the central rotating shaft of the present invention.

[0024] Figure 5 It is a schematic diagram of the test tube rack structure of the present invention.

[0025] Figure 6 It is a cross-sectional view of the central shaft structure of the present invention.

[0026] Figure 7 It is a structural cross-sectional view of the central rotation axis of the present invention at another angle.

[0027] Figure 1-7 Middle: 1- base, 2- operation panel, 3- sealing cover, 4- central shaft, 401- shaft body, 402- accommodating chamber, 403- guide block, 404- pressure regulating piston, 405- pressure relief hole, 406- ear plate, 407- piston rod, 5- centrifugal drum, 6- test tube rack, 601- collar, 602- bottom support, 603- connecting arm, 7- guide hose. DETAILED DESCRIPTION

[0028] The following will be combined with the accompanying drawings in the present invention Figure 1-Figure 7 , clearly and completely describe the specific technical solutions of the present invention;

[0029] See also Figure 1-Figure 7 , Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present invention; Figure 2 For the present invention Figure 1 A schematic diagram of the structure at A; Figure 3 This is a schematic diagram of the top cover of the present invention in the down state; Figure 4 It is a schematic diagram of the connection between the test tube rack and the central rotating shaft of the present invention; Figure 5 It is a schematic diagram of the test tube rack structure of the present invention; Figure 6 It is a cross-sectional view of the central shaft structure of the present invention; Figure 7 It is a structural cross-sectional view of the central rotation axis of the present invention at another angle.

[0030] The present embodiment provides a cell centrifuge for Chinese mitten crab experiments, comprising a base 1, a sealing cover 3 that is flipped open is arranged on the top of the base 1, a central shaft 4 that is connected to the high-speed motor in the base 1 is arranged in the center of the base 1, a centrifugal drum 5 that is driven to rotate by the central shaft 4 is fixedly sleeved on the outer side of the central shaft 4, and a test tube rack 6 is arranged on the centrifugal drum 5 to fix the experimental samples, characterized in that the top of the test tube rack 6 is hinged to the centrifugal drum 5, and the bottom is movably connected to the central shaft 4 through a telescopic piston rod 407;

[0031] By means of the hinged movable setting of the test tube rack 6, the overall angle of the test tube rack 6 can be changed by changing the bottom position of the test tube 6 under the centrifugal action. During the centrifugal movement, the test tube rack 6 drives the internal test tube to tilt due to the centrifugal force, and the centrifugal effect is maximized by the increase of the force arm. When it stops, it falls back due to the gravity. The vertical setting is convenient for taking, and also reduces the angle change when taking, reducing the degree of re-mixing and dissolution of the separated medium.

[0032] Among them, a groove is concavely provided on the front of the base 1, and an operating panel 2 for controlling the movement of the high-speed motor is embedded in the groove. When the sealing cover 3 is closed, the upper space of the groove is shielded. The concave operating panel 2 can not only prevent dust and protect the operating panel 2, but also the setting of the groove makes it easy to open the sealing plate 3 by hand, which is convenient for operation.

[0033] The surface of the centrifugal drum 5 is provided with a capsule-shaped groove perpendicular to the central rotating shaft 4, and the test tube rack 6 is arranged in the groove structure at one end close to the central rotating shaft 4, and the capsule-shaped groove provides space for the test tube rack 6 to change its angle.

[0034] Among them, the test tube rack 6 includes a ring 601, a base 602 and a connecting arm 603. The ring 601 is an annular structure for embracing and fixing the test tube. Horizontal connecting arms 603 are symmetrically arranged on both sides for hinged connection with the centrifugal drum 5. The lower side of the ring 601 is connected to a hemispherical base 602 for supporting the bottom of the test tube. A connecting arm 603 is vertically arranged at the bottom of the base for hinged connection with the piston rod 407.

[0035] The central shaft 4 includes a shaft body 401 and a piston rod 407. The shaft body 401 is a hollow shaft body, and a receiving chamber 402 is arranged inside. The receiving chamber 402 is filled with hydraulic oil medium. The outer side of the shaft body 401 is circumferentially arranged with ear plates 406. The piston rod 407 is hinged between the ear plates 406. The front end of the piston rod 407 is hinged to the bottom of the test tube rack 6. The oil chamber in the piston rod 407 is connected to the receiving chamber 402 in the shaft body 401 through the guide hose 7.

[0036] By connecting the accommodating chamber 402 with the piston rod 407 outside the accommodating chamber 402, when the central shaft 4 rotates, the test tube rack 6 moves toward a horizontal angle under the action of centrifugal force, and the angle change of the test tube rack 6 drives the piston rod 407 to expand. The hydraulic oil medium in the accommodating chamber 402 flows into the piston rod 407 under the action of negative pressure. By limiting the maximum flow rate of the guide hose 7, the expansion of the piston rod 407 can be hindered, that is, the angle change speed of the test tube rack 6 is controlled to prevent the angle from changing too fast to cause shock. After the centrifugation is completed, the falling speed of the test tube rack 6 can also be controlled. Finally, the test tube rack 6 and the test tube fall back to a vertical state under the action of gravity, which is convenient for taking and operation.

[0037] Specifically, the accommodating chamber 402 as a whole is a shuttle-shaped structure with a large diameter at the connection with the guide hose 7 and small diameters at the upper and lower ends. By utilizing the guide effect of the inclined surface of the shuttle-shaped structure, under the action of centrifugal force, the hydraulic oil medium will move more through the position of the guide short tube 7, thereby facilitating the deployment of the piston rod 407.

[0038] Furthermore, a guide block 403 structure protruding toward the center of the shaft body 401 is provided on the inner wall of the accommodating chamber 402, between adjacent guide hoses 7. Through the setting of the guide block 403, the hydraulic oil medium can be better guided to the position of the guide short tube 7 and enter the piston rod 407, thereby facilitating the deployment of the piston rod 407.

[0039] Furthermore, the bottom of the accommodating chamber 402 is connected to a vertically arranged channel, which is also filled with hydraulic oil medium. A pressure regulating piston 404 is slidably arranged at the bottom of the channel, and a pressure relief hole 405 communicating with the external atmospheric environment is penetrated and arranged on the side wall of the shaft body 401 under the pressure regulating piston 404;

[0040] By setting up the channel and the pressure regulating piston 404, when the hydraulic oil medium in the accommodating chamber 402 enters the piston rod 407 under the action of centrifugal force, the oil pressure in the accommodating chamber 402 decreases, which will drive the pressure regulating piston 404 to rise, and press the hydraulic oil medium in the channel into the accommodating chamber 402, thereby balancing the oil pressure in the accommodating chamber 402 and preventing the oil pressure from causing excessive obstruction to the deployment of the piston rod 407.

[0041] Furthermore, a protruding limiting structure is provided on the outer side of the pressure regulating piston 404 to cooperate with the shaft body 401 structure to limit the stroke of the pressure regulating piston 404 to prevent over-stroke movement. The stroke of the pressure regulating piston 404 can also be limited to control the change range of the hydraulic pressure in the accommodating chamber 402 and the piston rod 407, thereby flexibly controlling the stroke of the piston rod 407 through hydraulics. Compared with physically limiting the stroke of the piston rod 407, the recoil force when moving to the stroke limit position is reduced, thereby improving the stability of the device during operation.

[0042] When implementing the technical solution recorded in this embodiment, the sealing cover 3 is opened, the test tube is inserted into the test tube rack 6, and then the sealing cover 3 is closed. The operation panel 2 is operated to start the device. The central shaft 4 drives the centrifugal drum 5 and the test tube rack 6 to rotate under the drive of the motor. Through the centrifugal action, the bottom position of the test tube rack 6 changes, and the whole moves in the direction of the centrifugal force until it moves to the stroke center of the piston rod 407. The piston rod 407 is used to limit the angle, and the test tube rack 6 and the internal test tube are kept tilted to maximize the centrifugal action and centrifugal separation of the internal medium. After the centrifugation is completed, the device stops, and the test tube rack 6 slowly falls back to a vertical state under the action of gravity, which is convenient for taking and reduces the angle change during taking, thereby reducing the degree of mixing and dissolving of the separated medium again.

Claims

1. A cell centrifuge for Chinese mitten crab experiments, characterized in that: include: A machine base (1) is used to connect and fix the overall structure, and a sealing cover (3) that flips open is provided on the top of the machine base (1); A central rotating shaft (4), the base (1) is provided with a central rotating shaft (4) drivingly connected to a high-speed motor in the base (1), and a centrifugal drum (5) is fixedly sleeved on the outer side of the central rotating shaft (4) and driven to rotate by the central rotating shaft (4) to centrifugally drive the experimental sample; A test tube rack (6) is provided on the centrifugal drum (5) for fixing experimental samples. The top of the test tube rack (6) is hinged to the centrifugal drum (5), and the bottom is movably connected to the central shaft (4) through a telescopic piston rod (407) to change the angle. The central shaft (4) comprises: a shaft body (401), the shaft body (401) is a hollow shaft body, and a receiving chamber (402) is provided inside. The receiving chamber (402) is filled with hydraulic oil medium; a piston rod (407), the outer side of the shaft body (401) is circumferentially arranged with ear plates (406), the piston rod (407) is hinged between the ear plates (406), the front end of the piston rod (407) is hinged to the bottom of the test tube rack (6), and the oil chamber in the piston rod (407) is connected to the receiving chamber (402) in the shaft body (401) through a guide hose (7); The accommodating cavity (402) is generally in the form of a shuttle-shaped structure with a large diameter at the connection with the diversion hose (7) and small diameters at the upper and lower ends; The shaft body (401) further comprises: A guide block (403) is provided on the inner wall of the accommodating cavity (402) between adjacent guide hoses (7) with a guide block (403) structure protruding toward the central position of the shaft body (401); The central rotating shaft (4) also includes: The pressure regulating piston (404) is connected to a vertically arranged channel at the bottom of the accommodating chamber (402), and the channel is also filled with hydraulic oil medium. A pressure regulating piston (404) is slidably arranged at the bottom of the channel, and a pressure relief hole (405) communicating with the external atmospheric environment is penetrated and arranged on the side wall of the shaft body (401) at the lower side of the pressure regulating piston (404).

2. A cell centrifuge for Chinese mitten crab experiments according to claim 1, characterized in that: The base (1) further comprises: An operating panel (2) is provided on the front side of the machine base (1) with a groove, and an operating panel (2) for controlling the movement of the high-speed motor is embedded in the groove. When the sealing cover (3) is closed, the space above the groove is shielded.

3. The cell centrifuge for Chinese mitten crab experiment according to claim 1, characterized in that: The surface of the centrifugal drum (5) is provided with a capsule-shaped groove perpendicular to the central rotating shaft, and the test tube rack (6) is arranged in the groove structure at one end close to the central rotating shaft (4).

4. The cell centrifuge for Chinese mitten crab experiment according to claim 1, characterized in that: The test tube rack (6) comprises: The collar (601) is an annular structure and is used to surround and fix the top of the test tube. Transverse connecting arms (603) are symmetrically arranged on both sides and are used for hinged connection with the centrifugal drum (5); The bottom support (602) is connected to the lower side of the collar (601) with a hemispherical bottom support (602) for supporting the test tube. A connecting arm (603) is vertically arranged at the bottom of the bottom support (602) for hinged connection with the piston rod (407).

5. The cell centrifuge for Chinese mitten crab experiment according to claim 1, characterized in that: A protruding limiting structure is provided on the outer side of the pressure regulating piston (404) and cooperates with the shaft body (401) structure to limit the stroke of the pressure regulating piston (404).

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